CN105409845B - A kind of short hairs schizothoracin artificial fecundation method - Google Patents

A kind of short hairs schizothoracin artificial fecundation method Download PDF

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CN105409845B
CN105409845B CN201510863420.7A CN201510863420A CN105409845B CN 105409845 B CN105409845 B CN 105409845B CN 201510863420 A CN201510863420 A CN 201510863420A CN 105409845 B CN105409845 B CN 105409845B
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fish
water
parent population
feed
cylinder
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CN105409845A (en
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潘晓赋
王晓爱
杨君兴
刘倩
杨坤凤
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Kunming Institute of Zoology of CAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Fodder In General (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The present invention relates to a kind of method of short hairs schizothoracin artificial propagation, this method includes mainly bait preparing, parent population source, parent population is raised and train, parent population select, artificial insemination, hatch fish roe and fry seven steps of raising.The beneficial effects of the present invention are:Without using ocyodinic, short hairs schizothoracin can also lay eggs smart and can get higher fertility rate and hatchability;It is nonsynchronous fundamentally to solve the problems, such as that the agensis of wild fish male and female or development under environment are supported in the pool;Reduce the damage to short hairs schizothoracin parent population and reduces aquaculture cost;More rationally, efficiently, it improves hatching rate and reduces the waste of fish-egg resource;It improves the survival rate of fry while also reducing the abnormal rate of fry.

Description

A kind of short hairs schizothoracin artificial fecundation method
Technical field
The present invention relates to a kind of methods of the parent fish rearing of short hairs schizothoracin and artificial propagation, belong to artificial freshwater-fish breeding skill Art field.
Background technology
Short hairs schizothoracin (Schizothorax wangchiachii) is under the jurisdiction of Cypriniformes Cypriniforms, Cyprinidae Cyprinidae, Schizothoracinae Schizothoracinae, Schizothorax Schizothorax, also known as remote fish, husky tripe.It dwells The valley section in the river of highlands is ceased, is food with algae, Chang Jiqun lives belong to the middle-size and small-size cold water of Jinsha River system Property economic fish.Because of its fine and tender taste, delicious flavour, full of nutrition, liked by consumer very much.
In recent years, since habitat is destroyed, various unreasonable fishing means frequently use, and existing short hairs are split Abdomen fish resource amount has greatly reduced, and makes the aobvious costliness in its market price day.High market value and stock number in short supply so that grind Study carefully and a kind of efficiently brief short hairs schizothoracin artificial propagation techniques day shows important.
Although artificial freshwater-fish breeding technology has a long history, but still unresolved wild fish from wild environment into Enter the pool and support a series of problems occurred after environment, that stands in the breach in these problems surely belongs to reproductive dysfunction, and environment is supported on the pool Lower is difficult that mutually heat chases and the phenomenon that natural spawning before observing many Species of Rare Fish from Qingdao ovipositions, therefore wild Species of Rare Fish from Qingdao exists The pool, which is supported, is difficult to realize self procreation under environment.And for many fish, it is impossible to imitate the life in fish reproduction season completely Therefore state environment need to often inject efficient inducing agent for fish, pitocin induction fish oviposition production essence.The use of ocyodinic largely solves Determined artificial propagation of fish the problem of, can make fish oviposition production essence and can normal fertilization, obtain normal offspring, but fail to solve Root problem, we still place hope on parent fish rearing so that wild fish can natural spawning production essence in the case where environment is supported on the pool.
Though referring to parent fish rearing in existing patent and document, remaining in parent fish rearing is taken for the use of ocyodinic The primary stage of business, in a sense, this parent fish rearing are unsuccessful, and successful parent fish rearing is built upon pair On the basis that fish field ecology and reproductive habit extremely understand, and with pond culture experience for many years, to realize that fish exist Energy natural spawning production essence under environment is supported on the pool, to realize that self procreation of fish lays the foundation.By literature search, there is not related report Road.
Invention content
The purpose of the present invention is to provide a kind of short hairs schizothoracin parent fish rearing and artificial fecundation methods, to realize that short hairs are split Abdomen fish natural spawning production essence in the case where environment is supported on the pool, higher rate of fertilization is still can get without using ocyodinic, and unique fish incubate Change method can more rationally, efficiently utilize fish-egg, improve hatching rate, the raising stage by stage of fry also improves the survival of fry Rate.
According to an aspect of the present invention, a kind of short hairs schizothoracin parent fish rearing and artificial fecundation method, this method are provided Include the following steps:1) parent population acquires;2) parent population is raised and train:Parent population after disinfection is put into the raising and train in pond of sterilized cleaning, and Black shading net is capped on pond;Artificial synthesized pellet is fed daily twice;3) parent population selects:It is normal, raw to choose form Long good, the male and female parent population without disease is placed in glass jar, adjusts water flow velocity 20-25m/ minutes in glass jar, water quality is transparent Spend 100-105cm, dissolved oxygen 5.9-6.8mg/L, pH 7.0-7.4, osmotic pressure 2.8-4.7mOsm/L, 10-12 DEG C of temperature;4) people Work is inseminated:Physical health is picked out from glass jar, in good condition and sexually matured parent population carries out artificial insemination, in artificial insemination In the process, with osmotic pressure 272-298mOsm/L, the physiological saline of 11-12 DEG C of temperature gently rinses smart ovum mixture, and smart ovum is mixed It closes object and is positioned in hatching cylinder and hatch;5) hatch fish roe:Fish-egg is entered and hatches glass jar, injected clear water, the water surface is above fish-egg 4-5cm, hatching cylinder is interior to be irradiated without intense light source, 8-11 DEG C of water temperature, pH 7.0-7.4, dissolved oxygen 6.4-7.5mg/L, osmotic pressure 2.5- 3.5mOsm/L;6) fry is raised:Start to feed within 9 days after fry rupture of membranes, the feed phase feeds different feeds stage by stage, uses nitre daily Change bacterium and presses 15-17g/m2Regulating water quality.
Further, the stage is raised and train in parent population, the parent population after disinfection is put into the raising and train in pond of long 8m × wide 5m × high 3m, Potassium permanganate disinfection impregnates 1 day after long brush cleaning, and then clear water cleans 3 times, each 30-40min, after the completion of cleaning, to raising and train Water filling in pond makes to raise and train water level in pond and reaches 1.5m, flow velocity 75-85m/ minutes, water quality transparency 100-105cm, dissolved oxygen 5.7-6.5mg/L, pH 7.0-7.4, osmotic pressure 2.8-4.7mOsm/L, 12-16 DEG C of temperature.
Further, in the parent population choice phase, the male and female parent population of selection is placed in long 200cm × wide 100cm × high 100cm Tempered glass cylinder in, bottom is placed with one layer of cobblestone in cylinder, and it is 30-40cm to adjust height of water level in glass jar.
Further, the method for the artificial insemination is:It wipes in fish body after excessive moisture, using light pressure abdomen method, makes Ripe sperm and fish-egg flows out in clean container naturally, and smart ovum mixture is gently mixed 10-20 seconds with soft feather, quiet After setting 60 seconds, smart ovum mixture is gently rinsed with the physiological saline, is gently stirred clockwise with feather when rinsing It mixes, with sperm extra in the smart ovum mixture of removal, then smart ovum mixture is positioned in hatching cylinder and is hatched.
Further, in the hatch fish roe stage, using the hatching glass jar of long 50cm × wide 25cm × high 25cm, cylinder It is inside hung with the transparent plastic sheet of one piece of thickness 10mm, plastic plate is away from cylinder bottom face 5cm, and away from casing wall 2cm, plate is hollow and side has always Diameter is the gas injection port of 5mm, has the smooth arc groove to distribute as net shape, a diameter of 5mm in groove top edge adjacent on plastic plate There are the aperture of an a diameter of 1mm, aperture to be communicated with the hollow inside of transparent plastic sheet between groove, it is black that hatching cylinder bottom portion is lined with one Cloth;2500-4000, fish-egg is put per cylinder.
Further, the stage is raised in fry:The of feed phase feeds pulpous state bait sw for 1-4 days, and feeds mixing for 5-8 days Bait sw1;Feeds mixed feed sw2 for 9-15 days;Feeding volume is 8-10mL/m2;After fry is open 15 days, throw daily morning and evening Feed mixed feed sw3, feeding volume 8-10g/m2;Fry is open 30-45 days, feeds wheel animalcule and 701 carp kidney cell mixed feeds, Wheel animalcule feeding volume is 1,500,000/m2, 701 carp kidney cell mixed feed feeding volumes feed by feed coefficient for 1%-2%;It opens After mouthfuls 45 days, 701 carp kidney cell mixed feed feeding volumes are fed by feed coefficient for 3%-4%.Wherein, the pulpous state bait Sw includes:Cooked yolk, vitamine D3 calcium tablet, multivitamin and minerals tablet and water, mass ratio are cooked yolk:Vitamine D3 calcium tablet:It is more The first plain piece of dimension:Water=250:10:8:750.The mixed feed sw1 includes:Cooked yolk, now mill soya bean soya-bean milk, vitamine D3 Calcium tablet, multivitamin and minerals tablet and water, mass ratio are cooked yolk:Now grind soya bean soya-bean milk:Vitamine D3 calcium tablet:Multivitamin and minerals tablet:Water =250:250:10:8:500.The mixed feed sw2 includes:Now mill soya bean soya-bean milk, the mash feed containing 45% crude protein, dimension Raw element D3 calcium tablets, multivitamin and minerals tablet and water, mass ratio are now to grind soya bean soya-bean milk:Mash feed containing 45% crude protein:Vitamin D3 calcium tablets:Multivitamin and minerals tablet:Water=250:250:10:8:500.The mixed feed sw3 includes:Photosynthetic bacteria additive and fish Powder, the two mass ratio are 1:50.
The beneficial effects of the present invention are:
1. unused ocyodinic, short hairs schizothoracin can not only lay eggs smart and can get higher fertility rate and hatchability;
2. the success of short hairs schizothoracin parent fish rearing fundamentally solves the pool and supports wild fish male and female agensis under environment Or the nonsynchronous problem of development;
3. the zero of ocyodinic also reduces aquaculture cost using the damage not only reduced to short hairs schizothoracin parent population, operate It is more simple and efficient;
4. the hatch fish roe method of the present invention is arranged, more adduction for FAQs in short hairs schizothoracin hatch fish roe It manages, efficiently, improves hatching rate and reduce the waste of fish-egg resource;
5. the raising stage by stage of fry is set according to the different growth characteristics in short hairs schizothoracin fry each stage, not only carry The survival rate of high fry also reduces the abnormal rate of fry simultaneously.
Description of the drawings
Fig. 1 is the figure for showing influence of the water temperature to short hairs schizothoracin hatch fish roe.
Fig. 2 is the figure for showing influence of the osmotic pressure to short hairs schizothoracin hatch fish roe.
Fig. 3 is the figure for the influence for showing that different bait survive to short hairs schizothoracin fry.
Specific implementation mode
In from detailed description below, aforementioned aspect of the present invention and other aspects of the present invention will be apparent.
Embodiment 1
1. bait preparing
Pulpous state bait sw:Including cooked yolk, vitamine D3 calcium tablet, multivitamin and minerals tablet and water, mass ratio is cooked yolk: Vitamine D3 calcium tablet:Multivitamin and minerals tablet:Water=250:10:8:750.
Mixed feed sw1:Including cooked yolk, now grind soya bean soya-bean milk, vitamine D3 calcium tablet, multivitamin and minerals tablet and water, matter Amount is than being cooked yolk:Now grind soya bean soya-bean milk:Vitamine D3 calcium tablet:Multivitamin and minerals tablet:Water=250:250:10:8:500.
Mixed feed sw2:Including existing mill soya bean soya-bean milk, the mash feed containing 45% crude protein, vitamine D3 calcium tablet, multidimensional First plain piece and water, mass ratio are now to grind soya bean soya-bean milk:Mash feed containing 45% crude protein:Vitamine D3 calcium tablet:Muhivitamin Formula With Minerals Piece:Water=250:250:10:8:500.
Mixed feed sw3:Including photosynthetic bacteria additive and fish meal, the two mass ratio is 1:50.
2. parent population source
Parent population acquires in June, 2007 in Jinsha River system Lijiang City drum-shaped stone blocks town, is all made of the capture of three layers of linked network, and totally 173 Tail.The average value of sampled inspection, short hairs schizothoracin body length is 258mm, Weight averages 0.503kg.Parent population is packed into length 80cm, width 50cm plastics fish fry bags take back cultivation base, every bag of fish body weight 4.5-5kg after rushing oxygen.
3. parent population is raised and train
Long 8m, wide 5m, high 3m raise and train pond and empty and be exposed to the sun 3 days in advance, then clean pool wall with long brush, potassium permanganate is used in combination It impregnates 1 day, is then cleaned 3 times with clear water, it is 30-40 minutes each.It is put into after parent population is sterilized and raises and train pond, black screening is stamped on pond Light net, pool inner water position are 1.5m with drainage channel height outside pond, and water inlet pipe flow velocity is 75-85m/ minutes, water quality transparency 100-105cm.Oxygen supply equipment, dissolved oxygen 5.7-6.5mg/L, pH 7.0-7.4, osmotic pressure 2.8-4.7mOsm/ are installed in pond L, 12-16 DEG C of temperature.Daily 10:00 and 16:00 respectively to feed artificial synthesized pellet primary.After raising and train 5 days, total death parent population 23 tails.
4. parent population selects
On 2 12nd, 2014 09:00, physical health is chosen in pond from raising and train, form is good, unscathed short hairs schizothoracin Male and female parent population temporarily support in long 200cm, width 100cm, high 100cm tempered glass cylinder in, totally 45 tail.Bottom is placed with one layer of goose in cylinder Cobble, daily 10:00 to 16:Height of water level is 30-40cm in the 00 temporary vatting of adjustment, and parent population is enable to carry out the solarization back of the body, flow rate of water flow It it is 20-25m/ minutes, conditions above is that parent population simulates good spawning ground.Under the stimulation of various conditions, parent population property can be promoted Maturation is to lay eggs.Sexually matured parent population is mainly manifested in:Female abdomen is mellow and full to be expanded, soft-touch, and gonopore is more prominent; Male abdomen is less than normal, and the star-pursuing of head front end is apparent.Culvert system is set in cylinder, makes to keep water quality clean in entire cylinder, cylinder Interior water quality transparency is 100-105cm, dissolved oxygen 5.9-6.8mg/L, pH 7.0-7.4, osmotic pressure 2.8-4.7mOsm/L, temperature 10-12 DEG C of degree.
5. artificial insemination
17 days 2 months 16:00, pick out physical health from the temporary vatting of tempered glass, in good condition and ripe sexual gland 3 groups Parent population, every group of female-male proportion are 2:5.Excessive moisture in fish body is wiped, holds fish body back on the other hand, uses thumb and index finger edge on the other hand It ovary and spermary profile touches fish body abdomen and squeezes out fish-egg and sperm.The fish-egg and sperm of extrusion, are contained in clean container In, it is gently mixed smart ovum mixture 10-20 seconds with soft feather, stands 60 seconds.Then it is 272-298mOsm/ with osmotic pressure L, temperature are that 11-12 DEG C of physiological saline gently rinses smart ovum mixture, clockwise gently with feather in flushing Fish-egg sperm mixture is stirred to remove extra sperm.Fish-egg is positioned in hatching cylinder, obtains 31500, fish-egg altogether.
6. hatch fish roe
Hatching cylinder is the glass jar of long 50cm, width 25cm, high 25cm, hatches the transparent modeling that one piece of thickness 10mm is hung in cylinder Flitch, convenient for checking the hatching situation of fish-egg at any time;For plastic plate away from cylinder bottom face 5cm, edges of boards edge is 2cm at a distance from casing wall, Plate is hollow and there is the gas injection port of an a diameter of 5mm in side.There is the smooth arc groove to distribute as net shape on plastic plate, avoids rubbing Wiping makes fish-egg egg membrane rupture and when hatching because that fry twists is in heaps.The a diameter of 5mm in groove top edge has between adjacent grooves The aperture of one a diameter of 1mm, aperture are communicated with the hollow inside of transparent plastic sheet, and when hatch fish roe, oxygen is entered by gas injection port, Aperture outflow through net distribution, avoids reducing hatching rate because of the difference of local dissolution oxygen content.Every fish-egg is not in contact with each other And an exclusive groove, demersal egg can be prevented because of the growth of flow accumulation and mould.Hatching cylinder bottom portion is lined with a black cloth, convenient for incubating Dead ovum is chosen by transparent plastic sheet during change.When hatching, the water inlet on casing wall is consistent with cylinder bottom water outlet flow velocity, in stream Water hatches state.2500-4000, the fish-egg that ovum diameter is 2.37-3.25mm is put per cylinder, waits for that fish-egg enters cylinder, clear water, the water surface is added Above the ovum at 4-5cm.Hatch in cylinder that water temperature control is at 8-11 DEG C, pH 7.0-7.4, dissolved oxygen 6.4-7.5mg/L, osmotic pressure 2.5-3.5mOsm/L.To avoid fish-egg embryo from being generated lopsided fry by high light radiation, irradiated without intense light source in hatching house.By Totally 27200, smart fish-egg, rate of fertilization 86.3%, after 147 hours, fry starts to hatch hatch fish roe, after hatching 207 hours, Fry hatching terminates, and obtains 25150 tail of fry, hatching rate 92.5% altogether.
Fig. 1 is shown in influence of the temperature to short hairs schizothoracin hatch fish roe during hatch fish roe, the results show that with temperature It increases, hatching rate gradually rises, and abnormal rate continuously decreases, and when temperature is to 10 DEG C, hatching rate reaches highest, and abnormal rate is also down to most Low, temperature increases again, and hatching rate declines and abnormal rate rises.Therefore, hatch water temperature in cylinder in hatching process and be preferably controlled in 8- 11℃。
Fig. 2 is shown in influence of the osmotic pressure to short hairs schizothoracin hatch fish roe during hatch fish roe, the results show that with hatching It is gradually risen with the hatching rate of the raising of water penetration pressure, fish-egg, abnormal rate continuously decreases, when osmotic pressure is in 2.5-3.5mOsm/L When, hatching rate is higher, and abnormal rate is relatively low, and osmotic pressure increases again, then hatching rate declines, and abnormal rate rises.Therefore, in hatching process Osmotic pressure is preferably controlled in 2.5-3.5mOsm/L in hatching cylinder.
7. fry is raised
Start to feed within 9 days after fry rupture of membranes, that feeds the phase feeds pulpous state bait sw for first 4 days, and feeds mixed feed in 5-8 days Sw1, the feeds mixed feed sw2 for 9-15 days, and it is 8-10mL/m to feed metering2.After fry is open 15 days, feed daily morning and evening mixed Close bait sw3, feeding volume 8-10g/m2, while every morning 10:00 presses 15-17g/m with nitrobacteria2Regulating water quality.Fry Opening 30-45 days, feeds wheel animalcule and 701 carp kidney cell mixed feeds, and wheel animalcule feeding volume is 1,500,000/m2, 701 carp kidney cells Mixed feed feeding volume is fed for 1%-2% to calculate by feed coefficient.Opening can only feed 701 carp kidney cells and match after 45 days Feed is closed, feeding volume is fed for 3%-4% to calculate by feed coefficient.
Fig. 3 shows the influence that different bait survive to short hairs schizothoracin fry, wherein bait 1 and bait 2 split abdomen for short hairs The common bait of fish, 1 is fairy shrimp naupiar larva, and 2 be soft-boiled eggs Huang+wheel animalcule, and bait 3 is the above-mentioned bait combination of the present invention.Knot Fruit shows, compared to common bait, above-mentioned bait of the invention combines to have obtained higher seed survival rate, oral area abnormal rate It is relatively low.
Those skilled in the art is it should be understood that although for illustrative purposes, this document describes the tools of the present invention Body embodiment, but it can be carry out various modifications without departing from the spirit and scope of the present invention.Therefore, of the invention specific Embodiments and examples should not be considered as limiting the scope of the invention.The present invention is limited only by the appended claims.This Shen Please in quote all documents be fully incorporated herein by reference.

Claims (4)

1. a kind of short hairs schizothoracin artificial fecundation method, this method comprises the following steps:
1) parent population acquires;
2) parent population is raised and train:Parent population after disinfection is put into the raising and train in pond of sterilized cleaning, and is capped black shading net on pond; Artificial synthesized pellet is fed daily twice;
3) parent population selects:It chooses normal form, well-grown, the male and female parent population without disease to be placed in glass jar, adjusts glass jar Interior water flow velocity 20-25m/ minutes, water quality transparency 100-105cm, dissolved oxygen 5.9-6.8mg/L, pH 7.0-7.4, osmotic pressure 2.8-4.7mOsm/L, 10-12 DEG C of temperature;
4) artificial insemination:Physical health is picked out from glass jar, in good condition and sexually matured parent population carries out artificial insemination, In artificial insemination procedures, smart ovum mixture is gently rinsed with the physiological saline of osmotic pressure 272-298mOsm/L, 11-12 DEG C of temperature, Smart ovum mixture is positioned in hatching cylinder and is hatched;
5) hatch fish roe:Fish-egg is entered and hatches cylinder, injected clear water, water surface 4-5cm above fish-egg, hatching cylinder is interior to be shone without intense light source It penetrates, 8-11 DEG C of water temperature, pH 7.0-7.4, dissolved oxygen 6.4-7.5mg/L, osmotic pressure 2.5-3.5mOsm/L;Using long 50cm × wide The hatching cylinder of 25cm × high 25cm is hung with the transparent plastic sheet of one piece of thickness 10mm in cylinder, and plastic plate is away from cylinder bottom face 5cm, away from cylinder Wall 2cm, plate is hollow and there is the gas injection port of an a diameter of 5mm in side, has the smooth arc groove to distribute as net shape on plastic plate, The a diameter of 5mm in groove top edge, there is the aperture of an a diameter of 1mm between adjacent grooves, aperture and transparent plastic sheet it is hollow in Portion communicates, and hatching cylinder bottom portion is lined with a black cloth;2500-4000, fish-egg is put per cylinder;
6) fry is raised:Start to feed within 9 days after fry rupture of membranes, the feed phase feeds different feeds stage by stage, uses nitrobacteria daily By 15-17g/m2Regulating water quality;The of feed phase feeds pulpous state bait sw for 1-4 days, and feeds mixed feed sw1 for 5-8 days;9- Feed mixed feed sw2 within 15 days;Feeding volume is 8-10mL/m2;After fry is open 15 days, mixed feed is fed daily morning and evening Sw3, feeding volume 8-10g/m2;Fry is open 30-45 days, feeds wheel animalcule and 701 carp kidney cell mixed feeds, wheel animalcule feeding volume For 1,500,000/m2, 701 carp kidney cell mixed feed feeding volumes feed by feed coefficient for 1%-2%;After being open 45 days, 701 carp kidney cell mixed feed feeding volumes are fed by feed coefficient for 3%-4%,
Wherein, the pulpous state bait sw includes:Cooked yolk, vitamine D3 calcium tablet, multivitamin and minerals tablet and water, mass ratio are cooked Yolk:Vitamine D3 calcium tablet:Multivitamin and minerals tablet:Water=250:10:8:750,
The mixed feed sw1 includes:Cooked yolk, now mill soya bean soya-bean milk, vitamine D3 calcium tablet, multivitamin and minerals tablet and water, matter Amount is than being cooked yolk:Now grind soya bean soya-bean milk:Vitamine D3 calcium tablet:Multivitamin and minerals tablet:Water=250:250:10:8:500,
The mixed feed sw2 includes:Now mill soya bean soya-bean milk, the mash feed containing 45% crude protein, vitamine D3 calcium tablet, multidimensional First plain piece and water, mass ratio are now to grind soya bean soya-bean milk:Mash feed containing 45% crude protein:Vitamine D3 calcium tablet:Muhivitamin Formula With Minerals Piece:Water=250:250:10:8:500,
The mixed feed sw3 includes:Photosynthetic bacteria additive and fish meal, the two mass ratio are 1:50.
2. short hairs schizothoracin artificial fecundation method as described in claim 1, wherein the stage is raised and train in parent population, after disinfection Parent population is put into the raising and train in pond of long 8m × wide 5m × high 3m, after long brush cleaning potassium permanganate disinfection impregnate 1 day, then clear water cleans 3 times, each 30-40min, after the completion of cleaning, to water filling in pond is raised and train, makes to raise and train water level in pond and reach 1.5m, flow velocity 75-85m/ Minute, water quality transparency 100-105cm, dissolved oxygen 5.7-6.5mg/L, pH 7.0-7.4, osmotic pressure 2.8-4.7mOsm/L, temperature 12-16 DEG C of degree.
3. short hairs schizothoracin artificial fecundation method as described in claim 1, wherein in the parent population choice phase, by the female of selection Male parent population is placed in the glass jar of long 200cm × wide 100cm × high 100cm, and bottom is placed with one layer of cobblestone in cylinder, adjusts glass Height of water level is 30-40cm in cylinder.
4. short hairs schizothoracin artificial fecundation method as described in claim 1, wherein the method for the artificial insemination is:It wipes In fish body after excessive moisture, using light pressure abdomen method, ripe sperm and fish-egg is set to flow out in clean container naturally, with softness Feather be gently mixed smart ovum mixture 10-20 seconds, after standing 60 seconds, essence ovum mixture is gently rinsed with the physiological saline, It is gently mixed clockwise with feather when rinsing, with sperm extra in the smart ovum mixture of removal, then by smart ovum Mixture, which is positioned in hatching cylinder, hatches.
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CN106069927B (en) * 2016-06-24 2019-07-05 西藏自治区农牧科学院水产科学研究所 A kind of domesticating method of wild Lhasa schizothoracin
CN106577374A (en) * 2016-11-09 2017-04-26 中国水产科学研究院长江水产研究所 Domesticating method for wild euchiloglanis kishinouyei
CN107593536A (en) * 2017-11-13 2018-01-19 张荣富 A kind of schizothoracin breeds cultural method
CN107821244B (en) * 2017-11-16 2019-09-24 浙江省海洋水产研究所 A kind of little yellow croaker breeding method and feedstuff feeding device that ratio of female is high
CN108283152A (en) * 2018-03-28 2018-07-17 武汉中科瑞华生态科技股份有限公司 Artificial propagation method for schizothorax grahami
CN109644902B (en) * 2018-12-26 2021-05-07 中国水产科学研究院长江水产研究所 Artificial breeding method for schizothorax lasanensis
CN110295184A (en) * 2019-08-13 2019-10-01 水利部中国科学院水工程生态研究所 A kind of short hairs schizothoracin Avicennia marina, cloning process and its application
CN110663597B (en) * 2019-10-12 2021-04-23 长江水利委员会长江科学院 Site selection and construction method for muddy artificial spawning site of schizothorax prenanti in plateau river
CN110679522A (en) * 2019-11-12 2020-01-14 昆明倘浩水产养殖有限公司 Breeding method for schizothorax grahami fry opening stage
CN112352712B (en) * 2020-12-04 2022-06-07 中国科学院昆明动物研究所 Artificial propagation method of sinocyclocheilus rhinoceros
CN112352713B (en) * 2020-12-04 2022-06-07 中国科学院昆明动物研究所 Artificial propagation method for neolabeo fasciatus
CN112400761B (en) * 2020-12-04 2022-06-07 中国科学院昆明动物研究所 Sinocyclocheilus grahami and carp hybridization method
CN112741029B (en) * 2021-02-04 2022-06-07 中国科学院昆明动物研究所 Method for hybridizing sinocyclocheilus grahami and crucian carp
CN114391491A (en) * 2021-11-30 2022-04-26 华能龙开口水电有限公司 Scale culture method for schizothorax grahami
CN114342876B (en) * 2022-01-18 2022-12-16 中国科学院昆明动物研究所 Artificial propagation method of xenopus laevis

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1746959A1 (en) * 1990-02-22 1992-07-15 Тихоокеанский океанологический институт Дальневосточного отделения АН СССР Method of artificial fertilization of roe of salmon family
CN1561704A (en) * 2004-03-31 2005-01-12 马直科 Artificial breeding method for schizothorax
CN102077787A (en) * 2010-11-17 2011-06-01 贵州省毕节地区水产技术推广站 Artificial propagation technology for schizothorax grahami
CN103891654A (en) * 2014-04-14 2014-07-02 江苏中洋集团股份有限公司 Artificial breeding method of new species of Murray cods
CN103960188A (en) * 2014-05-20 2014-08-06 中国水产科学研究院长江水产研究所 Artificial spawning induction method for rhinogobio ventralis
CN104160993A (en) * 2014-08-04 2014-11-26 宜昌市水产技术推广站 Artificial propagation method of fry of paracobitis variegatus
CN104221958A (en) * 2014-08-19 2014-12-24 中国水产科学研究院长江水产研究所 Artificial spawning method for coreius guichenoti
CN104255614A (en) * 2014-10-13 2015-01-07 丽江滇江鱼类繁育有限公司 Artificial propagation method of schizothorax gymnocypris potanini firmispinatus

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09233965A (en) * 1996-03-02 1997-09-09 Keijirou Motoie Method for artificial fertilization and breeding for sea eel

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1746959A1 (en) * 1990-02-22 1992-07-15 Тихоокеанский океанологический институт Дальневосточного отделения АН СССР Method of artificial fertilization of roe of salmon family
CN1561704A (en) * 2004-03-31 2005-01-12 马直科 Artificial breeding method for schizothorax
CN102077787A (en) * 2010-11-17 2011-06-01 贵州省毕节地区水产技术推广站 Artificial propagation technology for schizothorax grahami
CN103891654A (en) * 2014-04-14 2014-07-02 江苏中洋集团股份有限公司 Artificial breeding method of new species of Murray cods
CN103960188A (en) * 2014-05-20 2014-08-06 中国水产科学研究院长江水产研究所 Artificial spawning induction method for rhinogobio ventralis
CN104160993A (en) * 2014-08-04 2014-11-26 宜昌市水产技术推广站 Artificial propagation method of fry of paracobitis variegatus
CN104221958A (en) * 2014-08-19 2014-12-24 中国水产科学研究院长江水产研究所 Artificial spawning method for coreius guichenoti
CN104255614A (en) * 2014-10-13 2015-01-07 丽江滇江鱼类繁育有限公司 Artificial propagation method of schizothorax gymnocypris potanini firmispinatus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
短须裂腹鱼规模化人工繁育技术研究;李光华等;《现代农业科技》;20140603(第10期);第259页左栏1材料与方法-第261页3讨论 *

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