CN103081828B - Method for artificially propagating parabramis pekinensis - Google Patents
Method for artificially propagating parabramis pekinensis Download PDFInfo
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Abstract
The invention relates to a method for artificially propagating parabramis pekinensis. The method comprises the following steps of: 1) selecting the source of parent fish; 2) breeding the parent fish; 3) artificially spawning and performing insemination, namely a) selecting the parent fish, b) collecting semen, taking eggs and checking, c) matching, d) setting water temperature of spawning, e) injecting odinagogue, and f) laying eggs; and 4) performing hatching management. By the means of enhancement of parent fish nutrient enhancement cultivation, flowing water stimulation regulation and the like, the gonad development and maturation of the parent fish and discharge can be promoted, the artificial spawning and insemination processes are standardized, the technical problem in the artificial propagation of parabramis pekinensis is effectively solved, the spawning rate of artificial propagation is 89 percent, the fertility rate is 83 percent, and the hatching rate is 83.5 percent. The method is easy and convenient to operate and high in applicability and can meet the requirement on the industrial batch propagation.
Description
Technical field
The present invention relates to technical field of aquaculture, relate in particular to the method for a kind of Changchun bream artificial propagation.
Background technology
The national each Main River Systems of Changchun bream distribution, its build is slender, delicious meat, fat content is low, is a kind of medium-sized economic fish of high-quality.But Changchun bream is as a kind of freshwater fish kind of applicable cultivation development, and research obviously lags behind, so far there are no the report that closes Changchun bream artificial fecundation method.The present invention mainly solves the method for carrying out Changchun bream breeding under manually operated condition, reaches the object of steady production Changchun bream seed, establishes seedling basis for promoting cultivation Changchun bream.
Summary of the invention
The object of this invention is to provide the method for a kind of Changchun bream artificial propagation, by strengthening the means such as parent population fortification cultivation, stimulation by running water regulation and control, promote parent population gonadal maturation and discharge, specification artificial induced spawning, insemination technique, break through a Changchun bream artificial propagation techniques difficult problem effectively; There is easy and simple to handle, spawning rate and incubation rate high, and can meet industrialization and breed in batches requirement.
The present invention is achieved in that the method for Changchun bream artificial propagation, it is characterized in that: comprise the following steps: 1). parent population source: breeding parent population is fill with the Changchun bream that Jiang Namiao raises and train or adopt the wild Changchun bream catching; 2). parent population seed selection: a, early stage, hydrostatic was cultivated: select physique stalwartness, vigor is good, fin squama is complete, without the anosis reserve parent fish of wound, put into the still-water pond of area more than 4 mu, breeding density is 400-500 tail/mu; Within under reserve parent fish behind pond 3 days, start the biological feed of throwing something and feeding; B, later stage flowing water reinforced cultivating: to the 2nd year winter, cultivated in the flowing water pond that reserve parent fish is proceeded to more than 6 mu from stilling basin, and breeding density is 300-350 tail/mu; The February in next year, flowing water pond bath per week 2～3 times, each 5-6 hour; Through reinforced cultivating half a year, male, dam's piscinity gland maturity; 3). artificial induced spawning, insemination: a, parent population are selected: by ripe raun fish body upwards, slightly dew face, ovary profile is obviously seen in both sides, presses belly, soft high resilience with have gentle hands, milter " pearl star " is obvious, and having very strong harsh feeling, fish body belly upwards, is gently pressed belly both sides, have milky dense thick seminal fluid to flow out, it is best meeting the water person that do not scatter rapidly; B, semen collection, dig ovum inspection: after drawing male parent population seminal fluid and activate with suction pipe, examine under a microscope sperm viability situation, ripe sperm has the rapid movement time of 30～50 seconds; Take out dam's fish-egg sample with digging egg apparatus, ovum sample is put into culture dish, add transparent immersion not have ovum sample, observe the form of ovum after 2～3 minutes, ripe ovum grain size is neat, full, whole or most egg nucleus off normals; C, combo: be 1 for female, the male parent population property ratio of breeding: 1.0-1: 1.2; D, the water temperature of hastening parturition: artificial induced spawning operates suitable water temperature at 20～26 DEG C; E, ocyodinic injection: adopt luteotropin releasing hormone d-ala analog LHRH-A
2, two kinds of ocyodinic compatibilities of human chorionic gonadtropin HCG hasten parturition to parent population; Every tail raun injected dose is LHRH-A
28 micrograms+HCG800IU, injection, is LHRH-A for the first time at twice
22 micrograms are LHRH-A for the second time
26 micrograms+HCG800IU, the double injection time interval is 8-11 hours; Milter is not injected for the first time, is a half-value dose for raun for the second time, and injection site is pectoral fin base portion; F, lay eggs: after oxytocic hormone injection, parent population is put into depth of water 1.2m, area is 20m
2spawning pond, the flow velocity that keeps water in spawning pond is 0.08 meter per second-0.12 meter per second, to stimulate parent population gonad development, at 20 DEG C-26 DEG C of water temperatures, Changchun bream was at 10-16 hour natural spawning; 4). hatching management: hatching is carried out in circular hatching channal, keeps the dissolved oxygen of water higher than 4 mg/litre between the incubation period, fry membrane 5-7 days, mouthpart, intestinal tube form, and fry culturing is carried out in the pool under fry.
The present invention is by strengthening the means such as parent population fortification cultivation, stimulation by running water regulation and control, promote parent population gonadal maturation and discharge, specification artificial induced spawning, insemination technique, effectively break through a Changchun bream artificial propagation techniques difficult problem, the spawning rate of artificial propagation is 89%, fertilization rate is 83%, and incubation rate is 83.5%.There is easy and simple to handle, applicability wide and can meet industrialization and breed in batches requirement.
Brief description of the drawings
Fig. 1 is the present invention's record sheet of hastening parturition;
Embodiment
The method of Changchun bream artificial propagation, is characterized in that: comprise the following steps: 1). parent population source: breeding parent population is fill with the Changchun bream that Jiang Namiao raises and train or adopt the wild Changchun bream catching; 2). parent population seed selection: a, early stage, hydrostatic was cultivated: select physique stalwartness, vigor is good, fin squama is complete, without the anosis reserve parent fish of wound, put into the still-water pond of area more than 4 mu, breeding density is 400-500 tail/mu; Within under reserve parent fish behind pond 3 days, start the biological feed of throwing something and feeding; B, later stage flowing water reinforced cultivating: to the 2nd year winter, cultivated in the flowing water pond that reserve parent fish is proceeded to more than 6 mu from stilling basin, and breeding density is 300-350 tail/mu; The February in next year, flowing water pond bath per week 2～3 times, each 5-6 hour; Through reinforced cultivating half a year, male, dam's piscinity gland maturity; 3). artificial induced spawning, insemination: a, parent population are selected: by ripe raun fish body upwards, slightly dew face, ovary profile is obviously seen in both sides, presses belly, soft high resilience with have gentle hands, milter " pearl star " is obvious, and having very strong harsh feeling, fish body belly upwards, is gently pressed belly both sides, have milky dense thick seminal fluid to flow out, it is best meeting the water person that do not scatter rapidly; B, semen collection, dig ovum inspection: after drawing male parent population seminal fluid and activate with suction pipe, examine under a microscope sperm viability situation, ripe sperm has the rapid movement time of 30～50 seconds; Take out dam's fish-egg sample with digging egg apparatus, ovum sample is put into culture dish, add transparent immersion not have ovum sample, observe the form of ovum after 2～3 minutes, ripe ovum grain size is neat, full, whole or most egg nucleus off normals; C, combo: be 1 for female, the male parent population property ratio of breeding: 1.0-1: 1.2; D, the water temperature of hastening parturition: artificial induced spawning operates suitable water temperature at 20～26 DEG C; E, ocyodinic injection: adopt luteotropin releasing hormone d-ala analog LHRH-A
2, two kinds of ocyodinic compatibilities of human chorionic gonadtropin HCG hasten parturition to parent population; Every tail raun injected dose is LHRH-A
28 micrograms+HCG800IU, injection, is LHRH-A for the first time at twice
22 micrograms are LHRH-A for the second time
26 micrograms+HCG800IU, the double injection time interval is 8-11 hours; Milter is not injected for the first time, is a half-value dose for raun for the second time, and injection site is pectoral fin base portion; F, lay eggs: after oxytocic hormone injection, parent population is put into depth of water 1.2m, area is 20m
2spawning pond, the flow velocity that keeps water in spawning pond is 0.08 meter per second-0.12 meter per second, to stimulate parent population gonad development, at 20 DEG C-26 DEG C of water temperatures, Changchun bream was at 10-16 hour natural spawning; 4). hatching management: hatching is carried out in circular hatching channal, keeps the dissolved oxygen of water higher than 4 mg/litre between the incubation period, fry membrane 5-7 days, mouthpart, intestinal tube form, and fry culturing is carried out in the pool under fry.When concrete enforcement, 1). parent population source: the quality of parent population quality is the key of artificial propagation success or failure, for guaranteeing that in artificial propagation, parent population can reach gonadal maturation, fish for from the Changjiang river autumn physique stalwartness, body surface without wound, the wild Changchun bream of specification 0.6 kilogram or more in support parent to carry out pond foster temporarily, for breeding next year.2). the cultivation of parent population: a adopts hydrostatic to cultivate autumn: select physique stalwartness, vigor is good, fin squama is complete, without the anosis reserve parent fish of wound, puts into the still-water pond of area more than 4 mu, breeding density is 400-500 tail/mu; Within under reserve parent fish behind pond 3 days, start the biological feed of throwing something and feeding.B flowing water in spring reinforced cultivating: to winter, cultivate in the flowing water pond that reserve parent fish is proceeded to more than 6 mu from stilling basin, and breeding density is 300-350 tail/mu; The February in next year, flowing water pond bath per week 2～3 times, each 5-6 hour; Stop bath the last week when approaching to hasten parturition, prevent that current from stimulating and miscarrying.The proteinaceous feed of the sufficient richness of throwing something and feeding during reinforced cultivating, ensures nutrition supply.Through reinforced cultivating half a year, Changchun bream parent population gonad development all can reach the IV end of term.3). the male and female identification of parent population: early or mid May, when water temperature reaches 22 DEG C of left and right, parent population substantially all reaches maturity, and starts to carry out artificial induced spawning.The good milter of gonad development almost whole body all has " star-pursuing " of white granular, on the fin ray with pectoral fin, anal fin, that head, kiss end, the gill cover etc. are located " star-pursuing " is many and close; First fin ray of pectoral fin obviously thickens and slightly bending; Fish body has harsh feeling, gently pushes belly adularescent seminal fluid flow out with have gentle hands.Raun head, pectoral fin etc. locate to have a small amount of " star-pursuing ", and pectoral fin the first fin ray is compared with thin and straight; Belly expands soft and full of elasticity, and cloacal aperture is micro-prominent and slightly red, and the raun of gonad maturity is gently pressed belly. the visible ovum grain in gonopore place.4). manual injection's ocyodinic: adopt luteotropin releasing hormone d-ala analog LHRH-A
2, two kinds of ocyodinic compatibilities of human chorionic gonadtropin HCG hasten parturition to parent population.Every tail raun injected dose is LHRH-A
28 micrograms+HCG800IU(international unit), injection, is LHRH-A for the first time at twice
22 micrograms are LHRH-A for the second time
26 micrograms+HCG800IU, the double injection time interval is 8-11 hours; Milter is not injected for the first time, is a half-value dose for raun for the second time.Injection site is pectoral fin base portion.5). lay eggs: after ocyodinic injection, the proportioning by female, male parent population property than 1: 1.1, puts into depth of water 1.2m, area is 20m
2spawning pond, the flow velocity that keeps water in spawning pond is 0.08 meter per second-0.12 meter per second, to stimulate parent population gonad development, realizes parent population egg mother cell and is transformed rapidly by IV phase to the V phase.24 DEG C-26 DEG C of water temperatures, there is excited phenomenon of oestrusing in Changchun bream at 10-12 hour, after acquiring a certain degree with regard to natural spawning.6). hatching: Changchun bream ovum belongs to noncohesive floating ovum, hatches and can obtain good hatching effect at Lotic hatching circuit.In hatching process, require dissolved oxygen more than 4 mg/litre, water temperature is advisable with 25 DEG C of left and right.
According to the method described above in the time of 20～26 DEG C of water temperatures, 60 groups of the parent populations of hastening parturition in two batches, 4,700,000, common property ovum, the mean spawning rate 89%, average fertilization rate reached 83%, incubation rate reaches 83.5%, hatches altogether fry 3,360,000 tails, hastens parturition and the results are shown in Figure 1.
Claims (1)
1. the method for Changchun bream artificial propagation, is characterized in that: comprise the following steps:
1) parent population source: breeding parent population is fill with the Changchun bream that Jiang Namiao raises and train or adopt the wild Changchun bream catching;
2) parent population seed selection: a, early stage, hydrostatic was cultivated: select physique stalwartness, vigor is good, fin squama is complete, without the anosis reserve parent fish of wound, put into the still-water pond of area more than 4 mu, breeding density is 400-500 tail/mu; Within under reserve parent fish behind pond 3 days, start the biological feed of throwing something and feeding; B, later stage flowing water reinforced cultivating: to the 2nd year winter, cultivated in the flowing water pond that reserve parent fish is proceeded to more than 6 mu from stilling basin, and breeding density is 300-350 tail/mu; The February in next year, flowing water pond bath per week 2～3 times, each 5-6 hour; Through reinforced cultivating half a year, male, dam's piscinity gland maturity;
3) artificial induced spawning, insemination: a, parent population are selected: by ripe raun fish body upwards, slightly dew face, ovary profile is obviously seen in both sides, presses belly, soft high resilience with have gentle hands, milter " pearl star " is obvious, and having very strong harsh feeling, fish body belly upwards, is gently pressed belly both sides, have milky dense thick seminal fluid to flow out, it is best meeting the water person that do not scatter rapidly;
B, semen collection, dig ovum inspection: after drawing male parent population seminal fluid and activate with suction pipe, examine under a microscope sperm viability situation, ripe sperm has the rapid movement time of 30～50 seconds; Take out dam's fish-egg sample with digging egg apparatus, ovum sample is put into culture dish, add transparent immersion not have ovum sample, observe the form of ovum after 2～3 minutes, ripe ovum grain size is neat, full, whole or most egg nucleus off normals;
C, combo: be 1 for female, the male parent population property ratio of breeding: 1.0-1: 1.2; D, the water temperature of hastening parturition: artificial induced spawning operates suitable water temperature at 20～26 DEG C;
Dosage and the method for e, injection ocyodinic: every tail raun injected dose is LHRH-A
28 micrograms+HCG800IU, injection, is LHRH-A for the first time at twice
22 micrograms are LHRH-A for the second time
26 micrograms+HCG800IU, the double injection time interval is 8-11 hours; Milter is not injected for the first time, is a half-value dose for raun for the second time, and injection site is pectoral fin base portion;
F, lay eggs: after oxytocic hormone injection parent population to put into 1.2 meters of the depth of waters, area be the spawning pond of 20 square metres, the flow velocity that keeps water in spawning pond is 0.08 meter per second-0.12 meter per second, to stimulate parent population gonad development, at 20 DEG C-26 DEG C of water temperatures, Changchun bream was at 10-16 hour natural spawning;
4) hatching management: hatching is carried out in circular hatching channal, keeps the dissolved oxygen of water higher than 4 mg/litre between the incubation period, fry membrane 5-7 days, mouthpart, intestinal tube form, and fry culturing is carried out in the pool under fry.
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| CN103404469B (en) * | 2013-08-26 | 2015-08-05 | 华能澜沧江水电有限公司 | The artificial fecundation method of a kind of Ju Po |
| CN107410113A (en) * | 2017-08-30 | 2017-12-01 | 安徽鸿运生态农业有限公司 | A kind of cultural method for improving Changchun bream growth rate |
| CN111053053A (en) * | 2019-12-11 | 2020-04-24 | 上海市水产研究所（上海市水产技术推广站） | Artificial breeding method of bream |
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| CN102124977A (en) * | 2011-04-15 | 2011-07-20 | 苏州市水产研究所有限公司 | Matching method for bream fish fries serving as sinipera chuatsi fries prophase bait |
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| CN102124977A (en) * | 2011-04-15 | 2011-07-20 | 苏州市水产研究所有限公司 | Matching method for bream fish fries serving as sinipera chuatsi fries prophase bait |
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