CN115349468A - Total artificial propagation method of gobiocyparidae - Google Patents
Total artificial propagation method of gobiocyparidae Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a total artificial propagation method of gobiocypris gobiocephalus, which belongs to the field of fish propagation, and is characterized in that wild fingerlings are captured from a natural water area, domestication and intensive cultivation is carried out at least 3-4 months in a cement pond, wild fingerlings with good sexual maturity are selected, and next-generation juvenile fishes are obtained by adopting links of exogenous hormone artificial spawning, semidry artificial insemination, debonding, artificial incubation, fry cultivation and the like; the first filial generation juvenile gobio is intercropped in the cultivating soil pond of the opsariichthys bidentis, the 1-year-old juvenile gobio can reach sexual maturity, the maturity rate is 100 percent, and the full artificial propagation is realized by utilizing the first filial generation parent fish to propagate the second filial generation offspring seeds. The invention breaks through the bottleneck of difficult source of mature wild rhinogobio ventralis parents, establishes the parent population of the total artificial propagation of the rhinogobio ventralis, and can provide seedling material basis and technical support for the large-scale culture of the rhinogobio ventralis.
Description
Technical Field
The invention belongs to the technical field of fish reproduction, and particularly relates to a full-artificial reproduction method of gobiocyparidae.
Background
Rhinogobio (Pseudogobio vaillanti) Belonging to Cypriniformes, cyprinidae, gobiobasiidae, and gobiogobioPseudogobio) The small-sized fish is a special demersal small fish in fresh water areas in the east Asia continental province, is slender and cylindrical, has small muscle spines and high meat content, is commonly called as 'spongia fish' and 'cutworm' and the like, has higher economic value and nutritional value, is deeply favored by people, has a quite important position in fresh water fish in rivers in China, and is one of the species with higher fishery value in mountainous areas in China.
In recent years, the natural resources of the gobio ventralis suddenly decline due to factors such as damage to the environment of over-fishing and habitat thereof, the natural resources of the gobio ventralis in partial waters such as Taihu lake water areas, huaihe river midstream and sand Tanodo river are nearly extinct, and the continuation of the natural resources faces serious threats. Therefore, the artificial propagation of the gobiocypris is broken through, the problem of fry supply is solved, the artificial culture and proliferation of the gobiocypris are developed, and the method has very important significance for promoting the fishery ecological environment maintenance and fishery sustainable development of the gobiocypris abortus.
The artificial propagation and fry breeding technology of the gobiocypris is concerned by more and more aquatic workers, is benthic brook fish, has unique biological characteristics, has strong stress response of the newly captured wild gobiocypris gobiocarpus, is not artificially domesticated and stabilized, and often fails in fry breeding modes of artificial induced spawning and artificial insemination by directly capturing the wild gobiocypris mature fry.
Disclosure of Invention
The invention aims to overcome the defects and provides the total artificial propagation method of the gobiocypris, the method is suitable for mass propagation of gobiocypris fry for resource proliferation or artificial culture, and a good foundation is laid for the protection of the gobiocypris natural resources and the development of artificial culture.
In order to achieve the purpose of the invention, the technical scheme provided by the invention is as follows:
a total artificial propagation method of gobiocyparidae comprises the following steps:
(1) Domesticating and breeding of wild gobiocypris: wild gobiocypris dabryanus captured from natural water area is bred in cement pond with breeding density of 1 kg/m 2 When in stocking, 3 per thousand of salt and 3-5 mg/L of Chinese gall are adopted for soaking and disinfection in a full pool, the Chinese gall is continuously soaked for 5-7 days and then is taken out of water for normal domestication, mixed granulated feed with the grain diameter of 1.5 mm, the crude protein of 41 percent, the crude fat of 6.5 percent, the ash content of 15.7 percent and the energy of 16.5 MJ/kg is fed, the feeding amount of the mixed granulated feed is gradually increased to 3 percent from 1 percent of the weight of the fish grown in the pond, and the domestication cultivation time lasts for 3-4 months;
(2) Artificial hastening parturition: selecting parents with good gonad development from wild gobiocyparidae fingerlings after domestication and cultivation when the water temperature is stabilized at 22-25 ℃, wherein the male-female ratio is 1; the exogenous hormone injection dosage and the injection mode are as follows: 800 to 1000 IU chorionic gonadotropin (HCG)/kg fish body weight and 3 to 5 mu g luteinizing hormone releasing hormone analogue (LRH-A) 2 ) The weight of each kg of fish is plus 3 to 5 mg of diospyrone maleate (DOM)/kg of fish weight, the injection is carried out at the base of the pectoral fin at one time, and the injection dosage of the male fish is halved; after injection, the fish is put into a net cage in a spawning pond for hydroentanglement for 14 to 16 hours, and the water temperature is 23.0 to 25.5 ℃;
(3) Artificial insemination and debonding: artificial insemination is carried out by adopting a semi-dry insemination mode, fertilized eggs are debonded by using 600 mg/L yellow mud water, and the specific operation steps are as follows: wiping off water on the abdomen of the female fish after artificial spawning induction, and slightly pressing the abdomen of the female fish to extrude eggs into a clean and dry container; meanwhile, extruding the sperms of the male fishes, flushing the sperms onto fish eggs by using 0.65% of normal saline, and carrying out artificial insemination by gently stirring for 20-30 s; washing crushed loess with 8-10 mg/L potassium permanganate aqueous solution to form slurry, filtering with a 80-mesh screen to remove residues, filtering with a cloth bag to form yellow mud for later use, preparing the yellow mud with 3 mg/L potassium permanganate aqueous solution into 800 g/L yellow mud water for debonding fertilized eggs, pouring the yellow mud together with the fertilized eggs into a 60-mesh small net cage, rinsing with clear water, counting, and placing into an incubation bucket for running water incubation;
(4) Artificial incubation: putting the fertilized eggs after the debonding into an incubation bucket for running water incubation, wherein the incubation density is 70 to 100 ten thousand grains/m 3 Adjusting the water inflow of the hatching barrel to be 25 to 30L/min so that all fish eggs can be suspended and billowed slowly in the water body of the hatching barrel;
(5) And (3) seedling cultivation: when the incubated fry has complete ability of smooth swimming, putting the fry into a culture pond for culture, and ecologically culturing the fries by adopting a mode of synchronously culturing rotifers, cladocera natural baits and compound feed in a soil pond; when the total biomass of rotifer and cladocera natural baits in the water body of the culture pond reaches 10 to 15 per ml, the gobiocypobius gobiocephalus fry can be put into the culture pond, and the stocking density of the gobiocobius gobiocephalus fry is 150 to 180 thousand feet per hm 2 (ii) a When the total biomass of the rotifer and cladocera natural baits in the water body of the cultivation pond is reduced to 2 to 3/ml, the crushed materials of the compound granular feed with 41 percent of crude protein need to be fed, and the feeding amount is gradually increased to 8 percent from 3 percent of the weight of the fry; after 50 to 60 days of cultivation, obtaining the gobiocypario fries with the body length specification of 3-5 cm;
(6) Breeding the first filial generation parent fish: and (3) intercropping the gobiocyparidae fries with the body length of 3 to 5 cm obtained in the step (5) in a culture pond of the opsariichthys bidens, wherein the intercropping density is 3750 tails per hm 2 The feed for the gobiocyprisio ventralis does not need to be fed independently;
(7) The realization of the full artificial propagation: and (5) obtaining a first generation parent of the gobiocyprisrarus with mature gonad development by using the operation method in the step (6), and repeating the steps (2) to (6) to obtain second generation offspring seeds so as to realize whole-body propagation.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a method for artificially domesticating wild gobiocypobius fingerlings, which eliminates the stress response of the wild gobiocobius fingerlings and solves the difficult problems that the fry breeding is difficult to be carried out by directly capturing the mature fingerlings of the wild gobiocobius to carry out artificial induced spawning and artificial insemination; provides an ecological intercropping mode of the gobio guigobio first-generation parent fish which is bred and cultured artificially and maturely in the gulfweed pond; the stable gobiocyparidae total artificial propagation technology is formed, gobiocyparidae total artificial propagation is realized for the first time at home and abroad, technical support and seed guarantee are provided for gobiocaridae proliferation and releasing and artificial breeding, and a solid foundation is laid for realizing large-scale artificial breeding and breeding industrialization development of gobiocaridae.
Detailed Description
The invention is further illustrated by the following examples, without restricting its scope.
The compound pellet feed comprises the following components in parts by weight: 43% of fish meal, 30% of bean pulp, 10% of peanut meal, 12% of wheat middling, 4% of yeast powder, 0.7% of monocalcium phosphate, 0.2% of fishing multivitamin and 0.1% of fishing multiore. The raw materials are crushed and granulated into granulated feed with the grain diameter phi of 1.5 mm, and the nutritional indexes are as follows: 41% of crude protein, 6.5% of crude fat, 15.7% of ash and 16.5 MJ/kg of energy.
Example one
In 12 months to 2020 months in 2019, 118.6 kg of wild gobiocypobilus guichenoti fingerling fish with average specification of 24.6 g/tail and total amount of 4820 tails are collected from Yangtze river water area in Fujian province, and wild gobiocobius guichenoti domestication cultivation and artificial breeding work are carried out by applying the method disclosed by the invention on a fish spawning site under Shuichang county in Fujian province. The mature wild gobiocyprisrarus 4168 tail is domesticated and cultured together, the average body mass is 24.9 g/tail, the average full length is 14.2 cm, and the average domesticated and cultured survival rate is 86.47%. The age of the sexual mature fish is 1 by sampling detection + The female fish sexual maturity coefficient is (14.38 +/-3.48)%, and the relative fecundity is (207.0 +/-50.2) grains/g. In 4-7 months in 2020, 4 batches are induced to spawn totally, 735 groups of wild parent fishes are induced to spawn successfully, 645 tails of female fishes are induced to spawn successfully, the average induction rate reaches 87.76%, 216.18 ten thousand gobiocyprisraria eggs are obtained, the average fertilization rate is 83.8%, 181.16 ten thousand fertilized eggs are obtained, 125 ten thousand tails of gobiocyprisraria fries are hatched, and the average hatching rate reaches 69.0%. Culturing for 50 to 60 days to co-culture gobiocyprisraria fries 23.5 thousands of tails with the specification of 3 to 5 cmThe average survival rate of the culture reaches 18.8 percent. The method comprises the following specific steps:
(1) Domesticating and breeding of wild gobiocypris species: respectively stocking the collected wild gobiocypris guichenoti in cement ponds for domestication and cultivation in 12 months to 2020 months in 2019, wherein the stocking density is 1 kg/m 2 When in stocking, 3 per thousand of salt and 3-5 mg/L of Chinese gall are adopted for soaking and disinfection in a full pool, the water temperature is controlled to be 16-19 ℃, water is drained after continuous soaking for 5-7 days for normal domestication, the water temperature is controlled to be 16-21 ℃, the pH value is controlled to be 6.8-7.9, dissolved oxygen is maintained to be 5.0-6.5 mg/L, the mixed granulated feed with the grain diameter of 1.5 mm, crude protein of 41%, crude fat of 6.5%, ash content of 15.7% and energy of 16.5 MJ/kg is fed, the feeding amount of the mixed granulated feed is gradually increased to 3% from 1% of the weight of the cultured fish in the pond, the domestication and the breeding are carried out to 2020 and the mature wild gobo-like fish are domesticated and cultured.
(2) Artificial hastening parturition: after 4 months in 2020, selecting parents with good gonad development from wild gobiocypobios guichenoti after domestication and cultivation when the water temperature is stabilized at 22-25 ℃, wherein the male-female ratio is 1 2 ) Carrying out artificial induced spawning on the exogenous oxytocin with the weight of each kg of fish plus 3 mg of diutanone maleate (DOM)/kg of fish, injecting at the base part of the pectoral fin at one time, and reducing the injection dosage of the male fish by half; after injection, putting the parent fish into a net cage in a spawning pond for running water stimulation for 14 to 16 hours, and controlling the water temperature to be 23.0 to 25.5 ℃.
(3) Artificial insemination and debonding: wiping water on the abdomen of the female fish subjected to artificial spawning induction, and slightly pressing the abdomen of the female fish to extrude eggs into a clean and dry container; meanwhile, extruding the male fish semen, flushing the male fish semen onto fish eggs by using 0.65% physiological saline, and carrying out artificial insemination in a semi-dry method mode by gently stirring for 20-30 s; washing crushed loess with 8-10 mg/L potassium permanganate solution to form slurry, filtering with a 80-mesh screen to remove residues, filtering with a cloth bag to form yellow mud for later use, preparing the yellow mud into 800 g/L yellow mud water with 3 mg/L potassium permanganate solution during debonding, pouring fertilized egg granules into the yellow mud slightly to perform debonding of fertilized eggs, pouring the yellow mud and the fertilized eggs into a 60-mesh small net cage, rinsing with clear water, counting, and placing into an incubation bucket for running water incubation.
(4) Artificial incubation: putting the debonded fertilized eggs into an incubation bucket for incubation in running water, wherein the incubation density is 70 to 100 ten thousand eggs/m 3 Adjusting the water inflow rate of an incubation bucket to be 30L/min so that all fish eggs can be suspended and boiled slowly in a water body of the incubation bucket, controlling the water temperature to be 22-24 ℃, controlling the pH value to be 7.2-7.5, and maintaining the dissolved oxygen to be 5-6 mg/L;
(5) And (3) seedling cultivation: when the hatched fry has the full ability of swimming, putting the fry into a culture pond for culture, and performing ecological seedling culture in a mode of synchronously culturing rotifers and cladocera natural baits and matching with granulated feeds by adopting a soil pond; when the total biomass of the natural feed for rotifers and cladocerans in the water body of the culture pond reaches 10 to 15 per ml, the gobiocypris gobiocephalus can be placed in the culture pond, and the stocking density is 150 to 180 ten thousand tails per hm 2 (ii) a When the total biomass of the rotifer and cladocera natural baits in the water body of the cultivation pond is reduced to 2 to 3/ml, crushed materials of mixed particle feed with 41 percent of crude protein need to be fed, and the feeding amount is gradually increased to 8 percent from 3 percent of the weight of the fry. After 50 to 60 days of cultivation, the gobiocyparidae fries with the body length specification of 3 to 5 cm can be obtained.
Example two
In the fish breeding field under the Achillea Changcheng county in Fujian province from 6 months to 2021 months in 2020, the first generation of gobiocypobio ventralis parents are successfully cultivated by utilizing the artificially bred wild first generation gobiocobio ventralis offspring seeds in a soil pond ecological intercropping mode by using the method disclosed by the invention, the mature 1580 tail of the first generation of gobiocobio ventralis parents is co-cultivated, the average body mass is 28.5 g/tail, the average total length is 14.3 cm, and the average cultivation survival rate is 63.2%. Through sampling detection, the female fish sexual maturation coefficient is (13.18 +/-1.14)%, and the relative egg carrying amount is (182.59 +/-15.84) grains/g.
The method comprises the following specific steps: artificially cultured wild gobiocypiocypris with body length of 3-5 cm the first generation of offspring seeds are intercropped in a breeding soil pond of the opsariichthys bidens, the intercropping density is 3750 tail/hm 2 The feed for the gobiocypario does not need to be fed independently. Because the life habits of the gobiocypris gobio and the opsariichthys bidens are completely different, the opsariichthys bidens is small-sized carnivorous fish in the brook, inhabit the upper layer of a water area, and can eat food under the condition of artificial cultureThe feed is combined, the gobiocypris gobio perches at the bottom layer of the water body, foraging in silt, developed rhynchophorus is provided, the ingestion mode is sucking type, algae and benthos are eaten on the gravel by scraping, the food is relatively wide, ecological complementary advantages are provided in the artificial breeding pond of the gobiocypris, although the gobiocypris is carnivorous fish, the size of the mudiocyparidae is not different from that of the gobiocyparidae, the gobiocyparicus cannot be threatened in the pond breeding, the algae and the benthos on the bottom layer of the artificial breeding pond of the gobiocyparidae provide rich natural bait for the gobiocyparidae, the nutritional requirements of the growth and development of the gobiocypobius are met, and the gobiocypobius feed does not need to be fed again.
EXAMPLE III
The mature parent of the gobiocypris guichenensis cultivated by the gobiocypris guichenensis offspring seed artificially bred in the year 2020 is used for artificially hastening spawning, artificial insemination and debonding by the method of the invention, and four batches of the gobiocypris guichenensis offspring parent 406 groups are artificially hastened spawning altogether, the average oxytocic rate reaches 83.74%, 100 ten thousand gobio-like second-generation roes and 88.3 ten thousand fertilized eggs are obtained, the average fertility rate reaches 88.3%, 55 ten thousand gobio-like second-generation water blossoms are obtained after hatching, the average hatching rate is 62.29%, and the total artificial propagation of the gobio-like gobio is realized. The specific reproduction steps are as follows:
(1) Artificial hastening parturition: the first generation parent of gobiocyparis gobiocidalis with mature gonad is cultivated in an ecological intercropping mode in a gulf fish culture pond, the male-female ratio is 1 2 ) Carrying out artificial induced spawning on the exogenous oxytocin with the weight of each kg of fish plus 5 mg of diutanone maleate (DOM)/kg of fish, injecting at the base part of the pectoral fin at one time, and reducing the injection dosage of the male fish by half; after injection, putting the parent fish into a net cage in a spawning pond for running water stimulation for 14 to 16 hours, and controlling the water temperature to be 23.0 to 25.0 ℃.
(2) Artificial insemination and debonding: wiping water on the abdomen of the female fish subjected to artificial spawning induction, and slightly pressing the abdomen of the female fish to extrude eggs into a clean and dry container; meanwhile, extruding the sperms of the male fish, flushing the sperms onto fish eggs by using 0.65% physiological saline, and slightly stirring for 20-30 s to carry out artificial insemination in a semi-dry method mode; washing crushed loess with 8-10 mg/L potassium permanganate solution to form slurry, filtering with a 80-mesh screen to remove residues, filtering with a cloth bag to form yellow mud for later use, preparing the yellow mud into 800 g/L yellow mud water with 3 mg/L potassium permanganate solution during debonding, pouring fertilized egg granules into the yellow mud slightly to debond fertilized eggs, pouring the yellow mud and the fertilized eggs into a 60-mesh small net cage, rinsing with clear water, counting, and placing into an incubation bucket for running water incubation.
(3) Artificial incubation: putting the debonded fertilized eggs into an incubation bucket for incubation in running water, wherein the incubation density is 70 to 100 ten thousand eggs/m 3 Adjusting the water inflow rate of an incubation bucket to be 28L/min so that all fish eggs can be suspended and boiled slowly in the water body of the incubation bucket, controlling the water temperature to be 23.0-25.0 ℃, controlling the pH value to be 6.9-7.6, and maintaining the dissolved oxygen to be 5-6 mg/L;
(4) And (3) seedling cultivation: when the hatched fry has the full ability of swimming, putting the fry into a culture pond for culture, and performing ecological seedling culture in a mode of synchronously culturing rotifers and cladocera natural baits and matching with compound feed by adopting a soil pond; when the total biomass of rotifer and cladocera natural baits in the water body of the culture pond reaches 10 to 15 per ml, the gobiocypiocypris gobio can be put into the culture pond, and the stocking density is 150 to 180 ten thousand fish per hm 2 (ii) a When the total biomass of the rotifer and cladocera natural baits in the water body of the culture pond is reduced to 2 to 3/ml, crushed materials of mixed particle feed with 41 percent of crude protein need to be fed, and the feeding amount is gradually increased to 8 percent from 3 percent of the weight of the fry. Through 50-60 days of cultivation, the second generation of gobiocypobio offspring seed with the body length specification of 3-5 cm can be obtained, and the full artificial reproduction of gobiocobio is realized.
The above description is only a preferred embodiment of the present invention, and all the equivalent changes and modifications made according to the claims of the present invention should be covered by the present invention.
Claims (3)
1. A total artificial propagation method of gobiocyparidae is characterized by comprising the following steps:
(1) Domesticating and breeding of wild gobiocypris species: wild gobiocypris dabryanus captured from natural water area is bred in cement pond with breeding density of 1 kg/m 2 3 per mill is adopted during stockingSalt and gallnut of 3 mg/L-5 mg/L are soaked in the whole pool for disinfection, the water is removed for normal domestication after continuous soaking for 5-7 days, the mixed granulated feed with the grain diameter phi of 1.5 mm, the crude protein of 41 percent, the crude fat of 6.5 percent, the ash content of 15.7 percent and the energy of 16.5 MJ/kg is fed during the domestication period, the feeding amount of the mixed granulated feed is gradually increased to 3 percent from 1 percent of the weight of the fish planted in the pond, and the domestication cultivation time is 3-4 months;
(2) Artificial hastening parturition: when the water temperature is stabilized to be above 22 ℃, selecting parents with good gonad development from the wild gobiocypobius gobiocypris bred and cultivated, wherein the male-female ratio is 1; the compatibility and the injection dosage of the exogenous hormone are as follows: 800-1000 IU chorionic gonadotropin/kg fish body weight + 3-5 mug luteinizing hormone releasing hormone analogue/kg fish body weight + 3-5 mg diospyrone maleate/kg fish body weight, one-time chest fin basal part injection is carried out, and the injection dosage of the male fish is halved; after spawning is induced, putting parent fishes into a net cage in a spawning pond, and carrying out water jet excitation for 14-16 h, wherein the water temperature is controlled at 23.0-25.5 ℃;
(3) Artificial insemination and debonding: artificial insemination is carried out by adopting a semi-dry insemination method, and fertilized eggs are debonded by yellow mud water;
(4) Artificial incubation: putting the debonded fertilized eggs into an incubation barrel for incubation in running water, wherein the incubation density is 70-100 ten thousand eggs/m 3 Adjusting the water inlet flow of the hatching barrel to enable all fish eggs to slowly suspend and tumble in the water body of the hatching barrel;
(5) And (3) seedling cultivation: when the incubated fry has complete ability of smooth swimming, putting the fry into a cultivating pool for cultivating, and adopting a mode of synchronously cultivating rotifers and cladocera natural baits and compound feed in a soil pool to perform ecological seedling cultivation; when the total biomass of the natural feed for rotifers and cladocerans in the water body of the culture pond reaches 10 to 15 per ml, the gobiocypris gobiocephalus can be placed in the culture pond, and the stocking density is 150 to 180 ten thousand tails per hm 2 (ii) a When the total biomass of rotifer and cladocera natural baits in the water body of the culture pond is reduced to 2 to 3/ml, feeding broken materials of mixed particle feed with 41 percent of crude protein, wherein the feeding amount is gradually increased to 8 percent from 3 percent of the weight of the fry; obtaining the gobiocyparidae fries with the body length of 3-5 cm after 50-60 days of cultivation;
(6) Breeding the first filial generation parent fish: culturing 3-5 cm long gobio ventralis fries cultured in the step (5) in a maroufish culture soil pond in a sleeving manner, wherein the sleeving culture density is 3750 tails per hm 2 The feed for the gobiocypario does not need to be fed independently;
(7) And (3) realizing full artificial propagation: and (5) obtaining a first generation parent of the gobiocyprisrarus with mature gonad development by using the operation method in the step (6), and repeating the steps (2) to (6) to obtain second generation offspring seeds so as to realize whole-body propagation.
2. The total artificial propagation method of gobiocypiocypris as claimed in claim 1, wherein the specific operation steps of the semidry insemination mode in step (3) are as follows: wiping water on the abdomen of the female fish subjected to artificial spawning induction, and slightly pressing the abdomen of the female fish to extrude eggs into a clean and dry container; meanwhile, the sperms of the male fish are extruded out, the sperms are washed on the fish eggs by using 0.65 percent of normal saline, and the mixture is gently stirred for 20 to 30 seconds for artificial insemination.
3. The total artificial propagation method of gobiocypiocypris as claimed in claim 1, wherein the specific operation steps of step (3) to unstick the fertilized ovum are: selecting crushed loess, elutriating the crushed loess into slurry by using 8-10 mg/L potassium permanganate aqueous solution, filtering the slurry by using a 80-mesh screen, removing residues, filtering the slurry by using a cloth bag to obtain yellow mud, and preparing 800 g/L yellow mud water by using 3 mg/L potassium permanganate aqueous solution during debonding to perform fertilized egg debonding.
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