CN109548713A - A kind of artificial fecundation method of stick flower fish - Google Patents

A kind of artificial fecundation method of stick flower fish Download PDF

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Publication number
CN109548713A
CN109548713A CN201811382518.0A CN201811382518A CN109548713A CN 109548713 A CN109548713 A CN 109548713A CN 201811382518 A CN201811382518 A CN 201811382518A CN 109548713 A CN109548713 A CN 109548713A
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CN
China
Prior art keywords
fish
parent population
water
stick flower
stick
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811382518.0A
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Chinese (zh)
Inventor
李正荣
李静
卢文轩
梁阳阳
崔凯
方婷
金家红
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fisheries Research Institute of Anhui AAS
Original Assignee
Fisheries Research Institute of Anhui AAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fisheries Research Institute of Anhui AAS filed Critical Fisheries Research Institute of Anhui AAS
Priority to CN201811382518.0A priority Critical patent/CN109548713A/en
Publication of CN109548713A publication Critical patent/CN109548713A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00Medicinal preparations containing organic active ingredients
    • A61K31/33Heterocyclic compounds
    • A61K31/395Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445Non condensed piperidines, e.g. piperocaine
    • A61K31/4523Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
    • A61K31/454Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. pimozide, domperidone
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/04Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/08Peptides having 5 to 11 amino acids
    • A61K38/09Luteinising hormone-releasing hormone [LHRH], i.e. Gonadotropin-releasing hormone [GnRH]; Related peptides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00Medicinal preparations characterised by special physical form
    • A61K9/0012Galenical forms characterised by the site of application
    • A61K9/0019Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The present invention relates to fish culture technical fields, in particular a kind of artificial fecundation method of stick flower fish, in the present invention, utilize the life habit of stick flower fish, to the breeding environment in stick flower making fish simulation field, facilitates in stick flower fish reproductive process that parent population pairing is rapidly completed, accelerate the hatching of fish-egg after stick flower fish-egg is collected by the way of artificial incubation device, the hatching rate of stick flower fish can be effectively improved, the nursing after hatching can accelerate juvenile fish to grow up.

Description

A kind of artificial fecundation method of stick flower fish
Technical field
The present invention relates to fish culture technical field, specially a kind of artificial fecundation method of stick flower fish.
Background technique
Stick flower fish is in reproductive process, due to the influence of environment and other factors, so that the stick flower fish oviposition of natural propagation Quantity is low, and hatching rate is low, therefore, proposes a kind of artificial fecundation method of stick flower fish regarding to the issue above.
Summary of the invention
The purpose of the present invention is to provide a kind of artificial fecundation methods of stick flower fish to be given using the life habit of stick flower fish The breeding environment in stick flower making fish simulation field facilitates in stick flower fish reproductive process that parent population pairing is rapidly completed, by stick flower fish The hatching for accelerating fish-egg after ovum collecting by the way of artificial incubation device can effectively improve the hatching rate of stick flower fish, after hatching Nursing can accelerate juvenile fish to grow up, to solve the problems mentioned in the above background technology.
To achieve the above object, the invention provides the following technical scheme:
A kind of artificial fecundation method of stick flower fish, specifically comprises the following steps:
1, aquaculture pond makes, and makes one large-scale fish jar, long 5-8m, wide 4-6m, high 1.2-1.5m, production using glass Pedestal, base top are laid with ceramic electrical backing, which connects temperature relay, which connects power supply, Fish jar is placed on pedestal by the setting temperature of the temperature relay at 20-23 DEG C, and one layer of goose soft rock, thickness are filled in fish jar In 15-20cm, be laid with one layer of fine sand on goose soft rock, thickness pours into water in 25-30cm in fish jar, the pH value of water 6-7 it Between, water plant is planted on fine sand;
2, apparatus of oxygen supply is set, by the gas supply end insertion fish jar of apparatus of oxygen supply, water oxygen level is maintained into 4-7 always Between milligram;
3, prepare feed, prepare enough life foods and fed, be with water flea, red worm and other living body bait artemias It is main;
4, female fish is selected, selection 1 year or more stick flower fish puts into aquaculture pond, and periodically feeds, and feeding frequency is one day Twice;
5, after parent population is selected, parent population is put into the Phenoxyethanol anesthesia liquid that concentration is 2%, is pulled out after parent population anesthesia, Oxytocic drug is injected, which is made of luteinizing hormone releasing hormone LRH-A3 and dioxone DOM, wherein female parent population Oxytocic drug proportion be luteinizing hormone releasing hormone LRH-A3 and the allotment ratio of dioxone DOM is 1-1.5:200-250, often Injection volume is 0.3ml, and the oxytocic drug proportion of male parent population is the tune of luteinizing hormone releasing hormone LRH-A3 and dioxone DOM Proportion is 0.5-0.7:100-150, and every injection volume is 0.3ml.
6, fish-egg is collected, and after the completion of parent population pairing, stick flower fish produces fish-egg in the cave that water plant or public fish dig, by milter It pulls out, collects fish-egg and be placed on incubator;
7, between 6-7, water temperature controls between 25-28 DEG C the pH value of water in incubator, after fry hatching, periodically throws Living body bait artemia is fed, frequency is 3-4 times on the one, until 25 to 30 days, start to feed other feeds.
Preferably, in the selection parent population, male and female accounting is 1:2.
Compared with prior art, the beneficial effects of the present invention are: this method gives stick flower using the life habit of stick flower fish Making fish simulates the breeding environment in field, facilitates in stick flower fish reproductive process that parent population pairing is rapidly completed, stick flower fish-egg is received The hatching for accelerating fish-egg after collection by the way of artificial incubation device can effectively improve the hatching rate of stick flower fish, the nursing after hatching Juvenile fish can be accelerated to grow up.
Specific embodiment
Below in conjunction with summary of the invention of the invention, technical solution in the embodiment of the present invention is carried out clearly and completely Description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on this hair Embodiment in bright, every other implementation obtained by those of ordinary skill in the art without making creative efforts Example, shall fall within the protection scope of the present invention.
The present invention provides a kind of technical solution:
A kind of artificial fecundation method of stick flower fish, specifically comprises the following steps:
1, electric heating piece, the ceramic electrical backing connect temperature relay, which connects power supply, the temperature relay Setting temperature at 20-23 DEG C, fish jar is placed on pedestal, fills one layer of goose soft rock in the fish jar, thickness in 15-20cm, One layer of fine sand is laid on goose soft rock, thickness pours into water, the pH value of water is between 6-7, on fine sand in 25-30cm in fish jar Plant water plant;
2, apparatus of oxygen supply is set, by the gas supply end insertion fish jar of apparatus of oxygen supply, water oxygen level is maintained into 4-7 always Between milligram;
3, prepare feed, prepare enough life foods and fed, be with water flea, red worm and other living body bait artemias It is main;
4, female fish is selected, selection 1 year or more stick flower fish puts into aquaculture pond, and periodically feeds, and feeding frequency is one day Twice;
5, after parent population is selected, parent population is put into the Phenoxyethanol anesthesia liquid that concentration is 2%, is pulled out after parent population anesthesia, Oxytocic drug is injected, which is made of luteinizing hormone releasing hormone LRH-A3 and dioxone DOM, wherein female parent population Oxytocic drug proportion be luteinizing hormone releasing hormone LRH-A3 and the allotment ratio of dioxone DOM is 1:230, every injection volume For 0.3ml, the oxytocic drug proportion of male parent population is luteinizing hormone releasing hormone LRH-A3 and the allotment of dioxone DOM is compared and is 0.6:120, every injection volume are 0.3ml.
6, fish-egg is collected, and after the completion of parent population pairing, stick flower fish produces fish-egg in the cave that water plant or public fish dig, by milter It pulls out, collects fish-egg and be placed on incubator;
7, between 6-7, water temperature controls between 25-28 DEG C the pH value of water in incubator, after fry hatching, periodically throws Living body bait artemia is fed, frequency is 3-4 times on the one, until 25 to 30 days, start to feed other feeds.
Further, it selects in parent population, male and female accounting is 1:2.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding And modification, the scope of the present invention is defined by the appended.

Claims (2)

1. a kind of artificial fecundation method of stick flower fish, specifically comprises the following steps:
1), aquaculture pond makes, and makes one large-scale fish jar, long 5-8m, wide 4-6m using glass, and high 1.2-1.5m makes pedestal, Base top is laid with ceramic electrical backing, which connects temperature relay, which connects power supply, the temperature Fish jar is placed on pedestal by the setting temperature of relay at 20-23 DEG C, one layer of goose soft rock is filled in fish jar, thickness is in 15- 20cm is laid with one layer of fine sand on goose soft rock, and thickness pours into water in 25-30cm in fish jar, the pH value of water between 6-7, Water plant is planted on fine sand;
2), apparatus of oxygen supply is set, by the gas supply end insertion fish jar of apparatus of oxygen supply, water oxygen level is maintained into 4-7 milli always Between grams per liter;
3), prepare feed, prepare enough life foods and fed, based on water flea, red worm and other living body bait artemias;
4), Selection parent, selection 1 year or more stick flower fish puts into aquaculture pond, and periodically feeds, and feeding frequency is one day two It is secondary;
5) after, parent population is selected, parent population is put into the Phenoxyethanol anesthesia liquid that concentration is 2%, pulls out, injects after parent population anesthesia Oxytocic drug, the oxytocic drug are made of luteinizing hormone releasing hormone LRH-A3 and dioxone DOM, wherein female parent population is urged Production drug ratio is luteinizing hormone releasing hormone LRH-A3 and the allotment ratio of dioxone DOM is 1-1.5:200-250, every injection Amount is 0.3ml, and the oxytocic drug proportion of male parent population is luteinizing hormone releasing hormone LRH-A3 and the allotment of dioxone DOM is compared and is 0.5-0.7:100-150, every injection volume are 0.3ml;
6), fish-egg is collected, and after the completion of parent population pairing, stick flower fish produces fish-egg in the cave that water plant or public fish dig, by milter It pulls out, collects fish-egg and be placed on incubator;
7), between 6-7, water temperature controls between 25-28 DEG C the pH value of water in incubator, after fry hatching, periodically feeds Living body bait artemia, frequency are 3-4 times on the one, until 25 to 30 days, start to feed other feeds.
2. the artificial fecundation method of stick flower fish according to claim 1, it is characterised in that: in the selection parent population, male and female Accounting is 1:2.
CN201811382518.0A 2018-11-20 2018-11-20 A kind of artificial fecundation method of stick flower fish Pending CN109548713A (en)

Priority Applications (1)

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CN201811382518.0A CN109548713A (en) 2018-11-20 2018-11-20 A kind of artificial fecundation method of stick flower fish

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Publications (1)

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CN109548713A true CN109548713A (en) 2019-04-02

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109937925A (en) * 2019-04-25 2019-06-28 中国水产科学研究院淡水渔业研究中心 A kind of artificial fecundation method of stick flower fish

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN204335582U (en) * 2014-11-27 2015-05-20 巫溪县菊红水产养殖专业合作社 Giant salamander Ecology breeding facility
CN104705232A (en) * 2015-04-10 2015-06-17 宁国市金东坊农业开发有限公司 Ecology-simulated artificial tor (foliter) brevifilis brevifilis breeding method by virtue of earthen pond
CN105432513A (en) * 2015-10-26 2016-03-30 华中农业大学 Method for artificial propagation of longnose gudgeon
KR20170069444A (en) * 2015-12-11 2017-06-21 김예준 incubation partition
CN206744308U (en) * 2017-05-17 2017-12-15 南安市科联专利应用服务有限公司 A kind of fish culture case for improving survival rate of fish fry
CN107683797A (en) * 2017-09-26 2018-02-13 张家港大禹农业科技有限公司 The artificial fecundation method of small shaped fish mouse fish

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN204335582U (en) * 2014-11-27 2015-05-20 巫溪县菊红水产养殖专业合作社 Giant salamander Ecology breeding facility
CN104705232A (en) * 2015-04-10 2015-06-17 宁国市金东坊农业开发有限公司 Ecology-simulated artificial tor (foliter) brevifilis brevifilis breeding method by virtue of earthen pond
CN105432513A (en) * 2015-10-26 2016-03-30 华中农业大学 Method for artificial propagation of longnose gudgeon
KR20170069444A (en) * 2015-12-11 2017-06-21 김예준 incubation partition
CN206744308U (en) * 2017-05-17 2017-12-15 南安市科联专利应用服务有限公司 A kind of fish culture case for improving survival rate of fish fry
CN107683797A (en) * 2017-09-26 2018-02-13 张家港大禹农业科技有限公司 The artificial fecundation method of small shaped fish mouse fish

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109937925A (en) * 2019-04-25 2019-06-28 中国水产科学研究院淡水渔业研究中心 A kind of artificial fecundation method of stick flower fish

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Application publication date: 20190402

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