CN107439431A - One kind induction Pu'an crucian carp allogynogenesis first filial generation method and application - Google Patents
One kind induction Pu'an crucian carp allogynogenesis first filial generation method and application Download PDFInfo
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- CN107439431A CN107439431A CN201710871986.3A CN201710871986A CN107439431A CN 107439431 A CN107439431 A CN 107439431A CN 201710871986 A CN201710871986 A CN 201710871986A CN 107439431 A CN107439431 A CN 107439431A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
- A61K38/09—Luteinising hormone-releasing hormone [LHRH], i.e. Gonadotropin-releasing hormone [GnRH]; Related peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Endocrinology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Environmental Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Reproductive Health (AREA)
- Immunology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Marine Sciences & Fisheries (AREA)
- Dermatology (AREA)
- Fodder In General (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides one kind induction Pu'an crucian carp allogynogenesis first filial generation method and application, it is related to cultural method field.One kind induction Pu'an crucian carp allogynogenesis first filial generation method, including：Artificial induced spawning, artificial insemination, hatch fish roe and fry rearing；Artificial induced spawning includes：Choose the ripe female parent population of Pu'an pond crucian carp, No. 2 injection fish human chorionic gonadtropin, ovulation induction element LRH A2With the hybrid medicine of amino acid injection, the Singuo Red Carp hero parent population of maturation is chosen, injects fish human chorionic gonadtropin, the female parent population of Pu'an pond crucian carp and Singuo Red Carp hero parent population after injection are put into spawning pond and wait to produce；During artificial insemination, the fish-egg of the female parent population of Pu'an pond crucian carp and the seminal fluid of Singuo Red Carp hero parent population are squeezed into container simultaneously.The fingerling obtained by the hybridization has that the speed of growth is fast, meat flavor is good, meat nutrition component content is high, premunition and the characteristics of strong adaptability, improves economic well-being of workers and staff.
Description
Method field
The present invention relates to cultural method field, and more particularly to a kind of induction Pu'an crucian carp allogynogenesis first filial generation method
And application.
Background method
With the progress of aquatic science method, artificial propagation of fish method is improving constantly, according to statistics, the cultivation of fish hybrid
Annual production, account for the 16% of global aquaculture gross annual output amount.Since the success of silver carp bighead artificial propagation first, the whole nation is each in China
Ground has done many research work in terms of economic freshwater fish hybridization breeding, obtains and larger enters in the cross combination of Nian classes etc.
Open up simultaneously application.Although China obtains certain achievement in the crossbreeding research of freshwater fish, but still in place of Shortcomings,
Therefore effective fish hybridization breeding method is studied, enriches the experience vacancy of fish hybridization breeding, obtain hybridizing breeding to aquatic products
Cultivation has important value.
The content of the invention
It is an object of the invention to provide one kind to induce Pu'an crucian carp allogynogenesis first filial generation method, is hybridized by this
To fingerling have that the speed of growth is fast, meat flavor is good, meat nutrition component content is high, premunition and the characteristics of strong adaptability,
Improve economic well-being of workers and staff.
Another object of the present invention is to provide a kind of application for inducing Pu'an crucian carp allogynogenesis first filial generation method.
The present invention solves the problems, such as its method using following methods scheme to realize.
The present invention proposes a kind of induction Pu'an crucian carp allogynogenesis first filial generation method, including：Artificial induced spawning, manually by
Essence, hatch fish roe and fry rearing；
Artificial induced spawning includes：Choose the ripe female parent population of Pu'an pond crucian carp, injection fish human chorionic gonadtropin, ovulation induction
No. 2 LRH-A of element2With the hybrid medicine of amino acid injection, the Singuo Red Carp hero parent population of maturation is chosen, fish is injected and is promoted with chorion
Gonadal hormone, the female parent population of Pu'an pond crucian carp and Singuo Red Carp hero parent population after injection are put into spawning pond and wait to produce；
During artificial insemination, the fish-egg of the female parent population of Pu'an pond crucian carp and the seminal fluid of Singuo Red Carp hero parent population are squeezed into container simultaneously
In.
The present invention proposes application of the above-mentioned induction Pu'an crucian carp allogynogenesis first filial generation method in terms of aquaculture.
A kind of induction Pu'an crucian carp allogynogenesis first filial generation method of the embodiment of the present invention and the beneficial effect of application are：
A kind of induction Pu'an crucian carp allogynogenesis first filial generation method provided by the invention, with the female parent population of Pu'an pond crucian carp with it is emerging
The red carp hero parent population of state injects fish human chorionic gonadtropin, ovulation induction element 2 as maternal and male parent to the female parent population of Pu'an pond crucian carp
Number LRH-A2With the hybrid medicine of amino acid injection, HCG and No. 2 LRH-A of ovulation induction element2Collective effect is hastened parturition to be increased with collaboration
The effect of effect, amino acid injection contain several amino acids and are favorably improved protein level in fish body, reduce HCG side effect,
Strengthen the health of the female parent population of Pu'an pond crucian carp, ensure the quality of fish-egg.Developed by the female parent population of Pu'an pond crucian carp and Singuo Red Carp hero parent population
Filial generation, the characteristics of not only keeping the meat flavour of crucian delicious, nutritious, while also have that omnivorousness, disease resistance is strong, survival rate
The advantages that high.
Embodiment
, below will be in the embodiment of the present invention to make the purpose of the embodiment of the present invention, method scheme and advantage clearer
Method scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, it is the conventional production that can be obtained by commercially available purchase
Product.
Have below to a kind of induction Pu'an crucian carp allogynogenesis first filial generation method of the embodiment of the present invention and application
Body explanation.
A kind of induction Pu'an crucian carp allogynogenesis first filial generation method provided in an embodiment of the present invention, including：
1. parent population, fishpond prepare
Choose Singuo Red Carp hero parent population and the female parent population of Pu'an pond crucian carp physical strong, disease-free and injury-free, up to age at sexual maturity.It is general
It is apomictic one kind to pacify pond crucian carp Natural Gynogenesiss, and Pu'an pond crucian carp ovum develops into new individual by the nucleus of itself.
Carp sperm, which enters in ovum, only to be play a part of to stimulate egg development, is not formed masculonucleus and is provided inhereditary material, its filial generation
Inhereditary material is entirely from thelykaryon.Singuo Red Carp male parent induces the filial generation of Pu'an pond crucian carp female parent allogynogenesis, not only keeps
The characteristics of meat flavour of crucian is delicious, nutritious, while also have the advantages that omnivorousness, disease resistance are strong, survival rate is high.
Parent population puts preceding 7~8d in a suitable place to breed, with quick lime 0.2kg/m2Concentration carry out parent fish rearing, spawning pond, hatchery and fish
Seedling cultivates sterilization.10d 0.2~0.3/m of quick lime before fry pond-inputting2Fry rearing pond is thoroughly cleaned up the pond, 7~8d after cleaning up the pond
It is deep that 40~50cm of water is irrigated afterwards, while applies rotted pig manure 0.3/m2Foster and apply fertilizer water quality.
Parent population enters carries out fish body soaking disinfection before pond with 10~20mg/L potassium permanganate.Male and female point pond is put in a suitable place to breed, sex ration
1:1~2.
2. parent population feeds
The feedstuff of parent population feeding is included：Fish meal 15%, dregs of beans 25%, wheat bran 20%, wheat-middlings 10%, rape cake
10%, cottonseed cake 10%, corn flour 7%, the mixture 3% of vitamin, inorganic salts and amino acid.Contain in raw material：Crude protein
>=30%, crude fibre≤10%, coarse ash≤15%, moisture≤12.5%, calcium 0.40~1.60%, total phosphorus >=1.2%, rely ammonia
Acid >=1.5%, salt 0.30%~1.20%.The feed nutrition enriches, and meets the nutritional need of parent population, is favorably improved fish-egg
And the quality of milt liquid.
3. artificial induced spawning
The parent population that maturation is chosen from parent fish pond is hastened parturition, and the Sex judging method of parent population is：Singuo Red Carp hero parent population
Body is long and narrow, larger, and breeding season chest, abdomeinal fin and the gill cover have star-pursuing, and mature brood fish belly is narrow and small and slightly hard, and gonopore is smaller,
Cave in, it is light to press belly to have seminal fluid outflow.Slightly higher, body is wide, small to carry on the back for the bodily form of the female parent population of Pu'an pond crucian carp, reproduction season mature brood fish
Belly more expands and soft, and ovary profile is obvious, and outward appearance is full, and gonopore is larger, slightly red and swollen, and evagination is light to press belly often
There is the outflow of ovum grain.
Using the method for disposable intrathecal, the female parent population injection fish human chorionic gonadtropin of per kilogram Pu'an pond crucian carp
[Gonadotrophi Chorionici (abbreviation HCG)] 700~900IU, 4~6 micrograms No. 2 LRH-A of ovulation induction element2With 5~
The hybrid medicine of the amino acid injection of 10 micrograms, per kilogram Singuo Red Carp hero parent population inject 700~900IU fish chorion
Promoting sexual gland hormone.
Human chorionic gonadtropin (HCG) is a kind of oxytocic drug, and the folliculus film maturing to promotion egg mother cell is big.
HCG is widely used in fish and hastened parturition, and the collaboration of other oxytocic drugs can also be coordinated to hasten parturition.No. 2 LRH-A of ovulation induction element2It can promote dynamic
Thing hypophysis Chiba discharges luteotropin and follicle-stimulating hormone, and the scope of application is big, and dosage range is larger, and side effect is smaller.In the present invention
In, using HCG and No. 2 LRH-A of ovulation induction element2Collective effect is hastened parturition, and has synergistic function, has effect of preferably hastening parturition
Fruit.Because HCG is using can produce side effect during excess so that adverse reaction occurs in fish body, in the present invention, while gives Pu'an silver
The female parent population injection amino acid injection of crucian carp.Amino acid injection contains several amino acids, such as proline, serine, alanine,
Protein level in fish body is favorably improved, with HCG and No. 2 LRH-A of ovulation induction element2Collective effect promotes to hasten parturition, while ensures general
Pacify the health of the female parent population of pond crucian carp, ensure the quality of fish-egg.
Before injection, medicine is formulated as to 0.5~1.5mL of per kilogram parent population liquid medicine injection dosage, the parent population after injection is put
Enter and wait to produce in spawning pond, keep spawning pond depth of water 0.5m, and pay attention to observing the dynamic of parent population, check the ripe situation of parent population.
4. artificial insemination
During waiting to produce, because spawning Effect time changes with the change of water temperature.When spawning pond water temperature remains 23
At~26 DEG C, the female parent population of Pu'an pond crucian carp after artificial induced spawning and Singuo Red Carp hero parent population treat 13~14h of production carry out manually by
Essence.When the temperature of spawning pond is maintained at 27~30 DEG C, the female parent population of Pu'an pond crucian carp and Singuo Red Carp after artificial induced spawning are male
Parent population treats that 10~12h of production carries out artificial insemination.The water temperature of spawning pond and time to be produced are controlled, maximizing obtains fish-egg and fish
Seminal fluid, the mistake of time is avoided to cause to lose part fish-egg and milt liquid.
When to when the time is produced, procuratorial work Pu'an pond crucian carp parent population, gently pressing its belly to have the outflow of ovum grain, that is, start artificial insemination,
Nominal kinship's fish body to be wiped during insemination, makees to contain ovum container with a diameter of 15cm clean anhydrous white magnetic bowl, coordinated, action is brisk,
While squeezing ovum, milter seminal fluid (1~2 drop seminal fluid of every ten thousand ovum) is squeezed into rapidly on one side, and be gently mixed 1~2 minute with feather,
Then physiological saline is added slowly, then is gently mixed 1~2 minute.
5. hatch fish roe
In order to provide a suitable environment of hatching to fish-egg, Chinese alpine rush is dried after washing, beam is bundled into and is banded in directly
Footpath is 3cm, is made fish nest (8~10 beam fish nests/bar) on a length of 2m bamboo bar, during use with 1 to 10 ten thousand liquor potassic permanganate
20min~30min is soaked, is then placed into being used as fish-egg attachment in the cement pit that the depth of water is 45~55cm；When adhering to fish-egg,
First water body is impacted with palm and fish-egg attachment produces spray (imitative fish produce the spray when laying eggs), it is rear slowly to obtain artificial insemination
The embryonated egg obtained, which is uniformly spilt, to be adhered on Chinese alpine rush.
The fish nest for being attached with fish-egg taking-up spawning pond is positioned on the support above hatchery, and timing is drenched to fish nest
Water, keep fish nest moistening and surrounding ventilation, check the embryonic development situation of fish-egg at any time, when visually see fish-egg occur two it is black
After the eyespot of color, i.e., fish nest is positioned in the water body of hatchery and hatched, keep the deep 45~55cm of water, water quality is pure and fresh, dissolved oxygen
Abundance, after prelarva whole membrane, fish nest is removed in good time, prevents water quality deterioration.
6. fry rearing
2~3kg/667m was pressed to the fries of 3~4 days after membrane2Scale of feeding feed 4~6wt% soya bean slurry.Per natural gift
Fed for 2 times, the time 8:00~9:00、15:00~16:00.Treat that fry rearing to 1.5~2cm specification, is gradually tamed and dociled
Eat man-made feeds.When fry specification reaches 4~5cm, you can raise in a point pond.
7. daily management
Strengthen daily pond inspection management, noted in good time according to pond water quality, fish movement situation, water temperature and climate change etc.
Enter new water, make water water quality is pure and fresh and the high and suitable water level of dissolved oxygen content, in favor of fish growth；At interval of 15~
20d measure pond fishes average weight 1 time, with this determination daily ration, feeding quantity；Strengthen fish disease prevention and cure, periodically used at interval of 15d around food field
2~3 baskets are hung in bleaching powder hanging basket 1 time, each field of eating, 0.2~0.3kg of dress bleaching powder per basket；Prepare Enrofloxacin HCL simultaneously
It is uniform with agricultural atomizer after medicine is dissolved by every 100kg pond fishes 3~5g of pure matter Enrofloxacin HCL with bait feeding
Ground is sprayed into pellet, is now fed with existing, daily each 1 time of morning and evening, and it is 1 course for the treatment of even to feed 3~d, it is found that fish disease diagnoses in time
Treatment.
Induction Pu'an crucian carp allogynogenesis first filial generation method provided by the invention, which has, promotes fish metabolism, enhancing
The characteristics of fish immunity ability and resistance against diseases, in terms of can be applied to aquaculture.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
A kind of induction Pu'an crucian carp allogynogenesis first filial generation method is present embodiments provided, including：
1. artificial induced spawning
The parent population that maturation is chosen from parent fish pond is hastened parturition, and medicine is formulated as to per kilogram parent population 0.5mL injection medicine
Liquor amount.Using the method for disposable intrathecal, the female parent population injection fish human chorionic gonadtropin of per kilogram Pu'an pond crucian carp
900IU, per kilogram Singuo Red Carp hero parent population inject 900IU fish human chorionic gonadtropin.Parent population after injection is put into production
Wait to produce in ovum pond.
2. artificial insemination
It is 24 DEG C to keep spawning pond water temperature, and the female parent population of Pu'an pond crucian carp after artificial induced spawning is treated with Singuo Red Carp hero parent population
13h is produced, the fish-egg of the female parent population of Pu'an pond crucian carp and the seminal fluid of Singuo Red Carp hero parent population are squeezed into container simultaneously, and is stirred with feather
1 minute, physiological saline is then added, is stirred for 1 minute.
3. hatch fish roe
The ovum of mixing is positioned on the Chinese alpine rush that the depth of water is 45cm with seminal fluid and hatched, Chinese alpine rush is before use through 1:
1×105Liquor potassic permanganate immersion 20min.
4. fry rearing
2kg/667m was pressed to the fries of 3~4 days after membrane2Scale of feeding feed 4wt% soya bean slurry, treat fry rearing extremely
1.5cm specification, food man-made feeds are gradually tamed and dociled, when fry specification reaches 4cm, you can raise in a point pond.
Embodiment 2
A kind of induction Pu'an crucian carp allogynogenesis first filial generation method is present embodiments provided, including：
1. artificial induced spawning
The parent population that maturation is chosen from parent fish pond is hastened parturition, and medicine is formulated as to per kilogram parent population 1.5mL injection medicine
Liquor amount.Using the method for disposable intrathecal, the female parent population injection fish human chorionic gonadtropin of per kilogram Pu'an pond crucian carp
No. 2 LRH-A of 700IU and the ovulation induction of 4 micrograms element2Hybrid medicine, per kilogram Singuo Red Carp hero parent population injection 700IU fish use
Human chorionic gonadtropin.Parent population after injection, which is put into spawning pond, waits to produce.
2. artificial insemination
When the temperature of spawning pond is maintained at 27 DEG C, the female parent population of Pu'an pond crucian carp and the male parent of Singuo Red Carp after artificial induced spawning
Fish 10h to be produced carries out artificial insemination, and the fish-egg of the female parent population of Pu'an pond crucian carp and the seminal fluid of Singuo Red Carp hero parent population are squeezed into appearance simultaneously
In device, and stirred 1~2 minute with feather, then add physiological saline, be stirred for 1~2 minute.
3. hatch fish roe
The ovum of mixing is positioned on the Chinese alpine rush that the depth of water is 55cm with seminal fluid and hatched, Chinese alpine rush is before use through 1:
1×105Liquor potassic permanganate immersion 30min.
4. fry rearing
3kg/667m was pressed to the fries of 3 days after membrane2Scale of feeding feed 6wt% soya bean slurry, treat fry rearing to 2cm
Specification, gradually tame and docile food man-made feeds, when fry specification reaches 4~5cm, you can raise in a point pond.
Embodiment 3
A kind of induction Pu'an crucian carp allogynogenesis first filial generation method is present embodiments provided, including：
1. artificial induced spawning
The parent population that maturation is chosen from parent fish pond is hastened parturition, and medicine is formulated as to per kilogram parent population 1mL liquid medicine injection
Dosage.Using the method for disposable intrathecal, the female parent population injection fish human chorionic gonadtropin of per kilogram Pu'an pond crucian carp
800IU, 5 micrograms No. 2 LRH-A of ovulation induction element2With the hybrid medicine of the amino acid injection of 5 micrograms, per kilogram Singuo Red Carp
Male parent population injection 800IU fish human chorionic gonadtropin.Parent population after injection, which is put into spawning pond, waits to produce.
2. artificial insemination
It is 26 DEG C to keep spawning pond water temperature, and the female parent population of Pu'an pond crucian carp after artificial induced spawning is treated with Singuo Red Carp hero parent population
12h is produced, the fish-egg of the female parent population of Pu'an pond crucian carp and the seminal fluid of Singuo Red Carp hero parent population are squeezed into container simultaneously, and is stirred with feather
1~2 minute, physiological saline is then added, is stirred for 1~2 minute.
3. hatch fish roe
The ovum of mixing is positioned on the Chinese alpine rush that the depth of water is 50cm with seminal fluid and hatched, Chinese alpine rush is before use through 1:
1×105Liquor potassic permanganate immersion 30min.
4. fry rearing
2kg/667m was pressed to the fries of 3 days after membrane2Scale of feeding feed 5wt% soya bean slurry, treat fry rearing to 1.5
~2cm specification, food man-made feeds are gradually tamed and dociled, when fry specification reaches 4~5cm, you can raise in a point pond.
The fry obtained to the induction Pu'an crucian carp allogynogenesis first filial generation method provided by embodiment 1~3 is carried out
Raising, after fry grows to maturation, cultured output is determined, it is as a result as follows：
Table 1 cultivates result
| Survival rate | Yield | Quality | |
| Embodiment 1 | 80% | 15.7kg | It is good |
| Embodiment 2 | 85% | 19.3kg | It is good |
| Embodiment 3 | 90% | 22.6kg | It is excellent |
As shown in Table 1, the fry that the induction Pu'an crucian carp allogynogenesis first filial generation method provided by embodiment 4 obtains
Yield, survival rate and quality after raising is big is higher, illustrates that the induced development methodological science that embodiment 3 provides is reasonable.
Choose the fry that the induction Pu'an crucian carp allogynogenesis first filial generation method that embodiment 1 and embodiment 3 provide obtains
Raised.Specifically, specification up to the allogynogenesis filial generation fingerling of the embodiment 3 of 2.3g/ tails, by 1216 tails/100m2
Breeding density be put into the experiment pool, it is total to launch the tail of fingerling 4500, weight 10.5kg.The embodiment 1 that 2.7g/ tails are launched on the control pool provides
Fingerling, it is total to launch the tail of fingerling 4600, weight 12.5kg.The potassium permanganate decoction dipping bath fish that it is 10mg/L with concentration that fingerling, which enters before pond,
5~10min of body.Feeding cultivation is carried out to the fingerling in the experiment pool and the control pool.The cultivation result calculated during harvest is：
Table 2 cultivates result
As shown in Table 2, the induction Pu'an crucian carp allogynogenesis first filial generation method cultivation provided by embodiment 3 survives
Rate is high, and weightening is big.Compared to embodiment 1, the medicine in the method for embodiment 3 to the female parent population injection of Pu'an pond crucian carp is fish fine hair
The mixture of film promoting sexual gland hormone, ovulation induction element 2 and amino acid, HCG and No. 2 LRH-A of ovulation induction element2Collective effect is hastened parturition,
With synergistic function, there is preferable spawning effect, and amino acid injection contains several amino acids, is favorably improved fish
Vivo protein is horizontal, reduces HCG side effect, strengthens the health of the female parent population of Pu'an pond crucian carp, ensures the quality of fish-egg, obtained fish
Seedling body health, fast growing, survival rate is high, while maintaining the merit of female parent, obtains the growth vigor of male parent.
Embodiments described above is part of the embodiment of the present invention, rather than whole embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiment in the present invention, this area commonsense method personnel are obtained under the premise of creative work is not made
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. one kind induction Pu'an crucian carp allogynogenesis first filial generation method, it is characterised in that including：Artificial induced spawning, artificial insemination,
Hatch fish roe and fry rearing；
The artificial induced spawning includes：Choose the ripe female parent population of Pu'an pond crucian carp, injection fish human chorionic gonadtropin, ovulation induction
No. 2 LRH-A of element2With the hybrid medicine of amino acid injection, the Singuo Red Carp hero parent population of maturation is chosen, fish is injected and is promoted with chorion
Gonadal hormone, the female parent population of the Pu'an pond crucian carp and the Singuo Red Carp hero parent population after injection are put into spawning pond and wait to produce；
During artificial insemination, the fish-egg of the female parent population of Pu'an pond crucian carp and the seminal fluid of the Singuo Red Carp hero parent population are squeezed into appearance simultaneously
In device.
2. induction Pu'an crucian carp allogynogenesis first filial generation method according to claim 1, it is characterised in that per kilogram institute
State the fish human chorionic gonadtropin, the ovulation induction of 4~6 micrograms that the female parent population of Pu'an pond crucian carp injects 700~900IU
No. 2 LRH-A of element2With the amino acid injection of 5~10 micrograms.
3. induction Pu'an crucian carp allogynogenesis first filial generation method according to claim 1, it is characterised in that per kilogram institute
State the fish human chorionic gonadtropin that Singuo Red Carp hero parent population injects 700~900IU.
4. the induction Pu'an crucian carp allogynogenesis first filial generation method according to Claims 2 or 3, it is characterised in that to institute
Before stating the female parent population of Pu'an pond crucian carp and Singuo Red Carp hero parent population injection medicine, the medicine is formulated as per kilogram parent population 0.5
~1.5mL liquid medicine injection dosage.
5. induction Pu'an crucian carp allogynogenesis first filial generation method according to claim 1, it is characterised in that the spawning
When the temperature in pond is maintained at 23~26 DEG C, the female parent population of Pu'an pond crucian carp and the male parent of the Singuo Red Carp after artificial induced spawning
Fish treats that 13~14h of production carries out artificial insemination.
6. induction Pu'an crucian carp allogynogenesis first filial generation method according to claim 1, it is characterised in that the spawning
When the temperature in pond is maintained at 27~30 DEG C, the female parent population of Pu'an pond crucian carp and the male parent of the Singuo Red Carp after artificial induced spawning
Fish treats that 10~12h of production carries out artificial insemination.
7. induction Pu'an crucian carp allogynogenesis first filial generation method according to claim 1, it is characterised in that also include：
When the fish-egg and the seminal fluid are squeezed into the container, stir 1~2 minute, 1~2 point is stirred for after adding physiological saline
Clock.
8. induction Pu'an crucian carp allogynogenesis first filial generation method according to claim 1, it is characterised in that the fish-egg
Hatching includes：The ovum of mixing is positioned on the Chinese alpine rush that the depth of water is 45~55cm with the seminal fluid and hatched, it is described
Chinese alpine rush is before use through 1:1×105Liquor potassic permanganate soak 20~30min.
9. induction Pu'an crucian carp allogynogenesis first filial generation method according to claim 8, it is characterised in that the fry
Cultivation includes：2~3kg/667m was pressed to the fries of 3~4 days after membrane2Scale of feeding feed 4~6wt% soya bean slurry.
10. the induction Pu'an crucian carp allogynogenesis first filial generation method as described in any one of claim 1 to 9 is in aquaculture side
The application in face.
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| CN114698575A (en) * | 2022-05-10 | 2022-07-05 | 湖南文理学院 | Breeding method for variegated carp heterogenous gynogenesis |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105432531A (en) * | 2015-12-30 | 2016-03-30 | 贵州大学 | Efficient Pu'an silver prussian carp propagation and hatching method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105432531A (en) * | 2015-12-30 | 2016-03-30 | 贵州大学 | Efficient Pu'an silver prussian carp propagation and hatching method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114698575A (en) * | 2022-05-10 | 2022-07-05 | 湖南文理学院 | Breeding method for variegated carp heterogenous gynogenesis |
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Application publication date: 20171208 |