CN101263793B - Artificial propagation method for advanced fry of yellow catfish - Google Patents
Artificial propagation method for advanced fry of yellow catfish Download PDFInfo
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- CN101263793B CN101263793B CN2008100111013A CN200810011101A CN101263793B CN 101263793 B CN101263793 B CN 101263793B CN 2008100111013 A CN2008100111013 A CN 2008100111013A CN 200810011101 A CN200810011101 A CN 200810011101A CN 101263793 B CN101263793 B CN 101263793B
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- hatching
- yellow catfish
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 241000376029 Tachysurus fulvidraco Species 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 42
- 241000251468 Actinopterygii Species 0.000 claims abstract description 35
- 230000012447 hatching Effects 0.000 claims abstract description 28
- 239000004568 cement Substances 0.000 claims abstract description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000005556 hormone Substances 0.000 claims abstract description 11
- 229940088597 hormone Drugs 0.000 claims abstract description 11
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 11
- 239000001301 oxygen Substances 0.000 claims abstract description 11
- 239000004576 sand Substances 0.000 claims abstract description 11
- 229920003023 plastic Polymers 0.000 claims abstract description 7
- 239000004033 plastic Substances 0.000 claims abstract description 7
- 238000002347 injection Methods 0.000 claims description 23
- 239000007924 injection Substances 0.000 claims description 23
- 230000037396 body weight Effects 0.000 claims description 9
- 210000004246 corpus luteum Anatomy 0.000 claims description 8
- 239000003488 releasing hormone Substances 0.000 claims description 8
- 210000004681 ovum Anatomy 0.000 claims description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 4
- 229910000831 Steel Inorganic materials 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 239000010959 steel Substances 0.000 claims description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 238000012856 packing Methods 0.000 claims description 2
- 239000002504 physiological saline solution Substances 0.000 claims description 2
- 230000001568 sexual effect Effects 0.000 claims description 2
- 238000007873 sieving Methods 0.000 claims description 2
- 235000014347 soups Nutrition 0.000 claims description 2
- 238000006213 oxygenation reaction Methods 0.000 claims 2
- 238000010790 dilution Methods 0.000 claims 1
- 239000012895 dilution Substances 0.000 claims 1
- 235000013601 eggs Nutrition 0.000 abstract description 5
- 230000016087 ovulation Effects 0.000 abstract 1
- 230000000384 rearing effect Effects 0.000 abstract 1
- DPWSRXJWCYEGIV-CWMZTGDLSA-N acetic acid;n-[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2r)-1-[[(2s)-1-[[(2s)-5-(diaminomethylideneamino)-1-[(2s)-2-(ethylcarbamoyl)pyrrolidin-1-yl]-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxoprop Chemical compound CC(O)=O.CC(O)=O.CCNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](C)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)C1NC(=O)CC1)CC1=CC=C(O)C=C1 DPWSRXJWCYEGIV-CWMZTGDLSA-N 0.000 description 4
- 230000002196 ecbolic effect Effects 0.000 description 3
- 238000001914 filtration Methods 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 238000011533 pre-incubation Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to an artificial propagation method of yellow catfish fry, which is characterized in that: sexually mature yellow catfish over three years are chosen as the parent fishes; the female and male ratio is 3:1; a plurality of barrels are arranged in a spawning pond as fish nests; parent fish density in the spawning pond is 1 to 2 groups/m2; each group of parent fishes uses a barrel; the sand with a thickness ranging form 1cm to 5cm is arranged at the bottom of the barrel for the debonding of fish eggs; in spawning season, exogenous hormone is injected to the basal behind the dorsal fins of the parent fishes to induce ovulation and spermiation; an inversed conical container with smooth inner wall is used as the hatching barrel; fertilized eggs are arranged in the hatching barrel for hatching in flowing water; the dissolved oxygen in the water of the hatching barrel is larger than 5.5 mg/L; when hatching is over, fry is arranged in a cement pool for temporary rearing for 40 to 70 hours, then the fry is packaged in plastic bags with water and oxygen. The artificial propagation method of yellow catfish fry has the advantages that, well developed yellow catfish fry can be acquired; the operation is simple; the utilization rate of parent fishes is high; scale production of yellow catfish fry can be realized, and the invention has significant meaning for the intensive culture of yellow catfish.
Description
Technical field
The present invention relates to the fresh-water fishes artificial propagation, a kind of large-scale artificial propagation method of advanced fry of yellow catfish.
Background technology
Yellow cartfish distributes extensively in China, receives the culturist and the consumer welcomes because of its adaptability is strong, Fresh & Tender in Texture, economic worth is high.Because wild fry supply is difficulty, waterman author has done more research to the artificial propagation yellow cartfish in recent years.Existing yellow cartfish artificial propagation techniques is selected, is provided with fish nest, hatching m and supports temporarily and done more detailed research parent population, provide a kind of easier yellow cartfish extensive breeding method.Shortcomings such as but this method exists the parent population utilization ratio not high, and the injectable drug consumption is bigger than normal, and cost is higher, and impurity is more in the fry of hatching, and the hatching survival rate is low are difficult to adapt to the demand that mass artificial is bred.
Summary of the invention
To the shortcoming of existing advanced fry of yellow catfish artificial propagation techniques, the present invention provides a kind of method of artificial propagation advanced fry of yellow catfish.
Main flow process of the present invention is: parent population selects, is provided with that fish nest, injection of hormone are hastened parturition, fish-egg cleans, hatch in hatching pail that fish-egg, fry are foster temporarily, fry catches.Its technical scheme is: (1) parent population is selected: when water temperature reaches 22～26 ℃, carry out; Parent population is by raun: milter=3: 1 combos requires 3 ages above sexual maturity yellow cartfishs as parent population, more than raun cabrage 60 restrains; Outward appearance reaches ripeness standard, more than milter cabrage 125 grams; (2) the fish nest is set: as the fish nest, every group of parent population be with a bucket with drum, bucket end tiling sand, and thickness is 1～5 centimetre, is used for the fish-egg unsticking; (3) injection hormone: raun is injected at twice, 11 hours at interval: corpus luteum releasing hormone A is urged in per kilogram fish body weight injection for the first time
2(LHRH-A
2) 2 micrograms, human chorionic gonadtropin (HCG) 200IU, the short corpus luteum releasing hormone A of per kilogram fish body weight injection for the second time
2(LHRH-A
2) 10 micrograms, 8.0 milligrams of Malaysia DOMs (DOM), human chorionic gonadtropin (HCG) 2000IU; Milter is injected once, and ID is 1/2 of the raun ID second time; (4) fish-egg cleans: fish-egg is taken out from the fish nest, eluriate with clear water, remove unfertilized egg, sand and other foreign material of white; (5) hatch fish roe: with the incubation vessel of inverted conical inner wall smooth as hatching pail; Throw in wherein handling clean ovum, feed through the hatching of the water the pond of filtering from the bucket bottom, oxygen in water is greater than 5.5 mg/litre; Water temperature is 22～28 ℃, hatches 60～90 hours; (6) fry is supported temporarily: the fry behind the demoulding is put into cement pit, and water depth is 0.15～0.5 meter in the cement pit, and water temperature is 22～28 ℃, supports 40～70 hours temporarily; (7) catch fry and packing: the employing diameter is that 1～2 cun steel wire strengthens corrugated hose, with the drop siphonage fry is sucked from cement pit in the net cage, puts into the plastic sack that adds water then, and seal package fills oxygen.
The present invention can obtain well-developed advanced fry of yellow catfish, and easy and simple to handle, adopts raun: the ratio of milter=3: 1, and the parent population availability is high; Fish nest method to set up is simple, and fish-egg can fall on the sand, is beneficial to unsticking; Especially flowing water hatching strengthens fry physique, has obviously improved survival rate, to the large-scale production of advanced fry of yellow catfish a kind of more efficient methods is provided.
Embodiment
When water temperature reaches 22～26 ℃, in the yellow cartfish of cultivating, select parent population, selection criteria is: sexually matured yellow cartfish more than 3 ages, and the raun cabrage is more than 60 grams, and outward appearance is that belly expands, and the ovary profile was obvious when head was upwards holded up; The milter cabrage is 3: 1 combos by female-male proportion more than 125 grams.
In water depth is 0.5～1.2 meter spawning pond, the fish nest is set, set-up mode is: at drum bottom tiling sand, thickness is 1～5 centimetre; Wherein drum is plastic cylinder or iron drum, and diameter is 20～50 centimetres, 20～60 centimetres of height, and sand needs through 20～40 mesh sievings.With the parent population injection oxytocic hormone that chooses, put into spawning pond then, putting fish density is every square metre of 1～2 group of parent population, and every group of parent population placed a fish nest.The injection consumption of oxytocic hormone is: raun is injected at twice, for the first time the short corpus luteum releasing hormone A of per kilogram fish body weight injection
2(LHRH-A
2) 2 micrograms and human chorionic gonadtropin (HCG) 200IU; Carry out the injection second time after 11 hours, for the second time the short corpus luteum releasing hormone A of per kilogram fish body weight injection
2(LHRH-A
2) 10 micrograms, 8.0 milligrams of Malaysia DOMs (DOM) and human chorionic gonadtropin (HCG) 2000IU; Inject to milter when injecting for raun for the second time, dosage is 1/2 of the raun ID second time.Oxytocic hormone dilutes with 0.9% physiological saline, and the consumption of liquid medicine injection is 1 milliliter of soup of per 100 gram fish body weight injections.
Injecting method is: the injection site is base portion behind the parent population dorsal fin, and needle forward and fish body are 45, and depth of needle is 1 centimetre.
When water temperature was 22～28 ℃, 10～20 hours parent populations in injection back began to lay eggs in the fish nest, and every batch of fish laid eggs 15～30 hours, and every barrel of egg laying amount is 8000～15000.Keep miniflow water in the spawning pond and fill oxygen, discharge is at 10～30 cubic metres/hour, and oxygen in water is greater than 5.5 mg/litre.Keep egg-laying environment quiet, lucifuge.
Lay eggs after the end, fish-egg is taken out from the fish nest, put into clear water and clean, remove the unfertilized egg of white, clean sand and other foreign material, put into hatching pail and hatch.Hatching pail is the inverted conical container of being made by galvanized sheet, and water inlet is established in its bottom, and its back cut diameter is 0.5～1.5 meter, and height is 1.0～2.0 meters, and the top is a delivery port, encloses tightly with 60 mesh sieve thin,tough silk, and hatching preincubation bucket will clean sterilization; It is 50～2,000,000/cubic metre water that fish-egg is put into density, feeds through the water the pond of filtering from the hatching pail bottom, and the water dissolved oxygen is greater than 5.5 mg/litre in the hatching pail; Fish-egg is suspended in the circulating water; Water temperature is 22～28 ℃, and hatching is accomplished after 60～90 hours, average incubation rate 82%.
After treating 90% above fry demoulding; Fry is put into temporary curing cement pond support temporarily, temporary curing cement pond inner wall smooth, 0.15～0.5 meter of the depth of water; Fry throw in density be 4～100,000 tails/square metre; With miniflow water and fill oxygen, the oxygen in water that makes cement pit is greater than 5.5 mg/litre in temporary curing cement pond, and maintenance water quality is pure and fresh.
Support temporarily after 40～70 hours, cut-off directly is that 1～2 cun steel wire strengthens corrugated hose and with the drop siphonage fry sucked in the net cage, and net cage is made by 60 mesh sieve thin,tough silk, tie up on framework, float on waterborne.Then fry is put into the plastic sack of dress water, plastic sack is wide to be 40～60 centimetres, and height is 80～100 centimetres, every packed advanced fry of yellow catfish 5～100,000 tails, and seal package fills oxygen.
Claims (4)
1. artificial propagation method for advanced fry of yellow catfish; Comprise that parent population is selected, injects hormone, the fish nest is set, fish-egg cleans, support, catch the fry step temporarily with hatching pail hatching, fry; It is characterized by: (1) parent population is selected: selecting above sexual maturity parent population in 3 ages, is 3: 1 combos by female-male proportion; (2) injection hormone: will urge corpus luteum releasing hormone A at spawning season
2, Malaysia DOM and human chorionic gonadtropin process injection with the dilution of 0.9% physiological saline, base portion injection behind its dorsal fin; (3) the fish nest is set: do the fish nest with drum, sand is put in the bucket bottom; (4) fish-egg cleans: eluriate with clear water, remove unfertilized egg, sand and other foreign material of white; (5) hatch with hatching pail: as hatching pail, ovum is thrown in wherein through-flow water hatching with the incubation vessel of inverted conical inner wall smooth; (6) fry is supported temporarily: behind the fry demoulding, with supporting temporarily in its input cement pit, the depth of water is 0.15～0.5 meter in the cement pit; (7) catch fry: strengthen corrugated hose with steel wire, fry is sucked in the net cage, pack then; Described be provided with the fish nest be with plastics or iron drum as the fish nest, 20～50 centimetres of diameters, 20～60 centimetres of height, at drum bottom tiling sand, thickness is 1～5 centimetre, sand needs through 20～40 mesh sievings; Water inlet is established in described hatching pail bottom, and its back cut diameter is 0.5～1.5 meter, and height is 1.0～2.0 meters, and the top is a delivery port, and delivery port encloses tightly with 60 mesh sieve thin,tough silk; Describedly catch fry and be to use 1～2 cun steel wire to strengthen corrugated hose, utilize the drop siphonage, fry is sucked in the net cage, net cage is made by 60 mesh sieve thin,tough silk, ties up on framework, to float on waterbornely, is incorporated with oxygenation packing in the plastic sack of water then.
2. a kind of artificial propagation method for advanced fry of yellow catfish according to claim 1 is characterized in that injecting hormone: (1) injection of hormone dosage: the drug dose for per kilogram fish body weight injection is: short corpus luteum releasing hormone A
212 micrograms, 8.0 milligrams of Malaysia DOMs and human chorionic gonadtropin 2200IU; (2) injection of hormone method: raun is injected at twice, 11 hours at interval: corpus luteum releasing hormone A is urged in per kilogram fish body weight injection for the first time
22 micrograms, human chorionic gonadtropin 200IU, the short corpus luteum releasing hormone A of per kilogram fish body weight injection for the second time
210 micrograms, 8.0 milligrams of Malaysia DOMs, human chorionic gonadtropin 2000IU; Milter is injected once, and ID is 1/2 of the raun ID second time; The consumption of liquid medicine injection is 1 milliliter of soup of per 100 gram fish body weight injections.
3. a kind of artificial propagation method for advanced fry of yellow catfish according to claim 1; It is characterized in that described is that ovum is rendered in the hatching pail with the hatching pail hatching; It is 50～2,000,000/cubic metre water that fish-egg is put into density, and the use water temperature is 22～28 ℃ a flowing water, by bucket bottom water flowing in hatching pail; Fish-egg is suspended in the flowing water, hatched 60～90 hours.
4. a kind of artificial propagation method for advanced fry of yellow catfish according to claim 1; It is characterized in that fry supports temporarily: after treating 90% above fry demoulding, fry is put into cement pit supports temporarily, fry throw in density press 4～100,000 tails/square metre; In cement pit with the oxygenation of miniflow water; Oxygen in water is greater than 5.5 mg/litre, and water temperature is 22～28 ℃, supports 40～70 hours temporarily.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100111013A CN101263793B (en) | 2008-04-18 | 2008-04-18 | Artificial propagation method for advanced fry of yellow catfish |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100111013A CN101263793B (en) | 2008-04-18 | 2008-04-18 | Artificial propagation method for advanced fry of yellow catfish |
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| Publication Number | Publication Date |
|---|---|
| CN101263793A CN101263793A (en) | 2008-09-17 |
| CN101263793B true CN101263793B (en) | 2012-08-01 |
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| CN2008100111013A Expired - Fee Related CN101263793B (en) | 2008-04-18 | 2008-04-18 | Artificial propagation method for advanced fry of yellow catfish |
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| CN101720700B (en) * | 2009-12-10 | 2012-09-12 | 天津农学院 | Method for incubating pelteobagrus fulvidraco fry efficiently in small water body |
| CN101822231B (en) * | 2010-05-21 | 2011-11-16 | 云南省水产技术推广站 | Hatching method for Sinocyclocheilus grahami fertilized egg |
| CN103039387A (en) * | 2012-12-19 | 2013-04-17 | 神农架林区楚源渔业有限公司 | Breeding method for largescale shoveljaw fish fries |
| CN103583431B (en) * | 2013-11-10 | 2015-01-07 | 华中农业大学 | Method for mainly culturing yellow catfishes and carrying out polyculture on other feeding fishes in pond |
| CN104904637A (en) * | 2015-06-11 | 2015-09-16 | 刘有文 | Method of cultivating fry of Pelteobagrus fulvidraco |
| CN106804477A (en) * | 2015-11-30 | 2017-06-09 | 宁陕县绿康农业生态科技有限公司 | A kind of artificial fecundation method of Pelteobagrus fulvidraco |
| CN105706982B (en) * | 2016-03-07 | 2019-02-26 | 山东景明海洋科技有限公司 | A kind of nest soil that yellow forehead egg hatching rate can be improved |
| CN105766711B (en) * | 2016-03-07 | 2019-03-26 | 怀远县渔业科技发展有限责任公司 | A kind of preparation method for the nest soil that yellow forehead egg hatching rate can be improved |
| CN106879502B (en) * | 2016-11-28 | 2018-01-19 | 眉山市人和渔业专业合作社 | Hybridizing method, the composite feed additive of female Pelteobagrus fulvidraco/pseudobagrus vachelli and male Pelteobagrus vachelli |
| CN106489801B (en) * | 2016-12-12 | 2019-05-24 | 福建省淡水水产研究所 | A kind of half pierces thick lip fish artificial seedling rearing's method |
| CN106614138B (en) * | 2016-12-21 | 2020-01-10 | 湖州市水产技术推广站 | Method for removing deformed fry of hybrid offspring of female pelteobagrus fulvidraco and male river pelteobagrus fulvidraco |
| CN107278981A (en) * | 2017-07-07 | 2017-10-24 | 佛山市蓝科养殖科技有限公司 | A kind of mating system for hybridizing yellow bone fish |
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| CN1425281A (en) * | 2001-12-14 | 2003-06-25 | 辽宁省淡水水产研究所 | Large-scale artificial breeding technology for yellow cartfish |
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