CN101779605A - Artificial impregnation, incubation and domestication method of Sinocyclocheilus tingi - Google Patents
Artificial impregnation, incubation and domestication method of Sinocyclocheilus tingi Download PDFInfo
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- CN101779605A CN101779605A CN201010114389A CN201010114389A CN101779605A CN 101779605 A CN101779605 A CN 101779605A CN 201010114389 A CN201010114389 A CN 201010114389A CN 201010114389 A CN201010114389 A CN 201010114389A CN 101779605 A CN101779605 A CN 101779605A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to an artificial impregnation, incubation and domestication method of Sinocyclocheilus tingi, belonging to the technical field of fish product cultivation. The method adopts wet process impregnation and comprises the following steps: placing a palm sheet to which germ cells are clung in 50ppm Saprolegnia Ling aqueous solution for sterilizing for 20min, taking out and suspending the palm sheet in an incubation pool with the area of 20m<2> for incubation; performing oxygenation by an oxygen pump, wherein the water temperature is 18-20 DEG C, the pH value is 7.5-8.0m, and the dissolved oxygen concentration in water is 6.5-8.0mg/L; sterilizing once every 24h by the same method, continuing to sterilize for 3 days, and finishing incubation by spending 144h; and retaining the domestication water level to be 1.0-1.2m, the water temperature to be 16-21 DEG C and the dissolved oxygen concentration in water to be 6.5-8.0mg/L, covering 2/3 of a pool surface by a shading mesh for shading, using a 40-mesh screen window mesh to intercept and prevent escaping, feeding aquatic insects and Tubificidae in the first 7 days, feeding powdered fodder which is doped with 10% of fish meal and has higher protein content for 15 days in a food conversion period, and feeding particle bait in which the crude protein content is 34% in a formative period. The invention has the advantages of low cost, simple and convenient operation, favorable growth of the Sinocyclocheilus tingi, high survival rate more than 95%, and capability of being used in large-scaled production.
Description
Technical field:
The present invention relates to a kind of artificial insemination, hatching and domesticating method of comforting celestial gold thread Barb, belong to fishery-ies product cultural technique field.
Background technology:
Comfort celestial gold thread Barb (Sinocyclocheilus tingi Fang) and have another name called red coat, pineapple fish.Only being distributed in the solution cavity of Fuxian Lake and periphery thereof, is the peculiar fish of Fuxian Lake.Its fine and tender taste, delicious flavour is the good merchantable brand of inconnu.Twentieth century is comforted celestial gold thread Barb and also occupy certain proportion the seventies in the catches of Fuxian Lake.Then, comfort the danger that celestial gold thread Barb faces resource exhaustion, only can also adopt the part fingerling in the dragon's pool protecting individually preferably because the intrusion and the people of denizen is overfishing.For celestial this precious resources of gold thread Barb is comforted in protection and development and use, the Yunnan Province development and the reform committee have set up rare indigenous fish child care study base in 2,000,000 yuans of project verification in 2004 and specific loans by Kunming Institute of Zoology, Chinese Academy of Sciences.By system research has been carried out in the biological property of comforting celestial gold thread Barb original seed, pond artificial domestication, artificial propagation etc.Achievement in research is not only to protection with to recover this precious resources significant, and makes the industrialization production of comforting celestial gold thread Barb become possibility.
By literature search, do not see and identical report of the present invention.
Summary of the invention:
The object of the invention is to recovering the peculiar rare economic fish of Yunnan Fuxian Lake---comfort celestial this precious resources of gold thread Barb, and make it become another new varieties that the pond is propagated artificially, produce for the industrialization, the intensification that realize comforting celestial gold thread Barb, a kind of artificial insemination, hatching and domesticating method of comforting celestial gold thread Barb is provided.
Artificial insemination method of comforting celestial gold thread Barb of the present invention adopts the wet method fertilization, and concrete steps are as follows:
A. select for use carp brain pendant (PG) and human chorionic gonadtropin (HCG) as ocyodinic, the ocyodinic consumption is per kilogram of body weight injection PG 2mg+HCG 200IU, raun is divided biphasic injection, 14 hours blanking times, injection volume is 20% of an accumulated dose for the first time, will remain the disposable injection of dosage for the second time; When carrying out injecting the female second time, carry out disposable injection by 1/2 of raun injection total amount to male;
B. injected back 16 hours for the second time, earlier raun is pushed belly gently, ovum is clamp-oned in the container, put into clear water and covered ovum, in the ratio of male and female the milter sperm is clamp-oned wherein again than 2: 1, speed is cleaned 3 times with clear water after stirring 20 seconds with chicken feather is light, the fertilized egg after cleaning evenly is sprinkled upon on the brown sheet waits to hatch.
The artificial incubation method of comforting celestial gold thread Barb of the present invention is: the brown sheet that will adhere to fertilized egg is put into the water mold Lingshui Spring solution sterilization 20 minutes of 50ppm, takes out and hangs over 20m
2Hatch in the hatchery; Water temperature 18-20 ℃, pH 7.5-8.0 carries out oxygenation with the oxygen pump, dissolved oxygen in water 6.5-8.0mg/L; Every 24 hours with the sterilization of same method once, continuous sterilization 3 days was finished hatching in 144 hours.
The concrete steps of artificial domestication method of comforting celestial gold thread Barb of the present invention are as follows:
The pond is raised and train in a, selection, and water level remains on 1.0-1.2m, and water temperature 16-21 ℃, dissolved oxygen in water＞6mg/L; Hide pond face 2/3 shading with shading net, and escape with the interception of 40 order screen window nets is anti-at delivery port;
B, feed are selected and the mode of throwing something and feeding:
Laundering period: in the laundering period at initial stage of being transplanted to the pond artificial domestication, aquatic insect of throwing something and feeding (from other rich water pond, fishing for) and Limnodrilus hoffmeisteri (commercially available); Feeding volume is 2.5% of a fish body gross weight, and the time of throwing something and feeding is 7 days;
Change the food phase: according to comforting the carnivorous partially feeding habits characteristics of celestial gold thread Barb, select for use the higher mash feed of protein content to mix 10% fish meal as bait, proportioning is 9: 1, and feeding volume is 2.5% of a fish body gross weight, and the time of throwing something and feeding is 15 days;
The typing phase: select 111 series of Tongwei Co., Ltd. for use, diameter is 1.0-2.0mm, the granular bait for fish of crude protein content 34%; One bu is early, middle and late throws something and feeds 3 times, and the time is 9:00,17:00,21:00, and dinner suitably more feeds, and a day feeding volume is 3% of a body weight.
The present invention has that cost is low, easy and simple to handle, and the incubation rate height is comforted celestial gold thread Barb well-grown, and survival rate reaches the advantage more than 95%, can be used for large-scale production.
Embodiment:
Embodiment 1:
Artificial insemination method of comforting celestial gold thread Barb of the present invention adopts the wet method fertilization, and concrete steps are as follows:
A.3-4 the moon, select the ovary profile obvious, the loose expansion of gonopore, the parent population that slightly takes on a red color.Anatomic observation: ripe good parent population, ovum grain size is neat, is yellow, transparent glossy, is the good parent population of maturation.
B. select for use carp brain pendant (PG) and human chorionic gonadtropin (HCG) as ocyodinic, the ocyodinic consumption is per kilogram of body weight injection PG 2mg+HCG 200IU, raun is divided biphasic injection, 14 hours blanking times, injection volume is 20% of an accumulated dose for the first time, will remain the disposable injection of dosage for the second time; When carrying out injecting the female second time, carry out disposable injection by 1/2 of raun injection total amount to male.
B. injected back 16 hours for the second time, earlier raun is pushed belly gently, ovum is clamp-oned in the container, put into clear water and covered ovum, in the ratio of male and female the milter sperm is clamp-oned wherein again than 2: 1, speed is cleaned 3 times with clear water after stirring 20 seconds with chicken feather is light, the fertilized egg after cleaning evenly is sprinkled upon on the brown sheet waits to hatch.
Embodiment 2:
The artificial incubation method of comforting celestial gold thread Barb of the present invention is:
The brown sheet that is adhering to fertilized egg is put into the water mold Lingshui Spring solution of 50ppm and sterilized 20 minutes, take out and hang over 20m
2Hatch in the hatchery.Water temperature 18-20 ℃, pH 7.5-8.0 carries out oxygenation with the oxygen pump, dissolved oxygen in water 6.5-8.0mg/L; Every 24 hours with the sterilization of above-mentioned same method once, continuous sterilization 3 days was finished hatching in 144 hours.
Practical application shows: artificial insemination of the present invention and hatching m not only cost are low, easy and simple to handle, and gold thread Barb fertilization rate and incubation rate height, can be used for large-scale production.
Embodiment 3:
In June, 2008, (apart from 2 kilometers of Fuxian Lakes) catches to such an extent that comfort celestial gold thread Barb 300 tails from siron pool, Dengjiang County, (body long 91.8 ± 21.3) mm, body weight (15.7 ± 13.2) g, transplant in Kunming Institute of Zoology, Chinese Academy of Sciences rare indigenous fish child care study base and raise and train 20m
21 mouthful of rectangle cement pit, water level remains on 1.0-1.2m, and water temperature 16-21 ℃, dissolved oxygen in water 6.5-8.0mg/L.Hide pond face 2/3 shading with shading net, can prevent also that simultaneously fish jumps out, and escape with the interception of 40 order screen window nets is anti-at delivery port.
Feed is selected and the mode of throwing something and feeding:
Laundering period: in the laundering period at initial stage of being transplanted to the pond artificial domestication, aquatic insect of throwing something and feeding (from other rich water pond, fishing for) and Limnodrilus hoffmeisteri (commercially available); Feeding volume is 2.5% of a fish body gross weight, and the time of throwing something and feeding is 7 days;
Change the food phase: according to comforting the carnivorous partially feeding habits characteristics of celestial gold thread Barb, select for use the higher mash feed of protein content to mix 10% fish meal as bait, proportioning is 9: 1, and feeding volume is 2.5% of a fish body gross weight, and the time of throwing something and feeding is 15 days;
The typing phase: select 111 series of Tongwei Co., Ltd. for use, diameter is 1.0-2.0mm, the granular bait for fish of crude protein content 34%; One bu is early, middle and late throws something and feeds 3 times, and the time is 9:00,17:00,21:00, and dinner suitably more feeds, and a day feeding volume is 3% of a body weight.
Practical application shows: this domesticating method not only cost is low, easy and simple to handle, and comforts celestial gold thread Barb well-grown, and survival rate reaches more than 95%, can be used for large-scale production.
Claims (3)
1. an artificial insemination method of comforting celestial gold thread Barb is characterized in that comforting celestial gold thread Barb artificial insemination method and adopts the wet method fertilization, and concrete steps are as follows:
A. select for use carp brain pendant and human chorionic gonadtropin as ocyodinic, the ocyodinic consumption is per kilogram of body weight injection PG 2mg+HCG 200IU, and raun is divided biphasic injection, 14 hours blanking times, injection volume is 20% of an accumulated dose for the first time, will remain the disposable injection of dosage for the second time; When carrying out injecting the female second time, carry out disposable injection by 1/2 of raun injection total amount to male;
B. injected back 16 hours for the second time, earlier raun is pushed belly gently, ovum is clamp-oned in the container, put into clear water and covered ovum, in the ratio of male and female the milter sperm is clamp-oned wherein again than 2: 1, speed is cleaned 3 times with clear water after stirring 20 seconds with chicken feather is light, the fertilized egg after cleaning evenly is sprinkled upon on the brown sheet waits to hatch.
2. artificial incubation method of comforting celestial gold thread Barb is characterized in that comforting celestial gold thread Barb artificial incubation method is: the brown sheet that will adhere to fertilized egg is put into the water mold Lingshui Spring solution sterilization 20 minutes of 50ppm, takes out and hangs over 20m
2Hatch in the hatchery; Water temperature 18-20 ℃, pH 7.5-8.0 carries out oxygenation with the oxygen pump, dissolved oxygen in water 6.5-8.0mg/L; Every 24 hours with the sterilization of same method once, continuous sterilization 3 days was finished hatching in 144 hours.
3. artificial domestication method of comforting celestial gold thread Barb, the concrete steps of artificial domestication method that it is characterized in that comforting celestial gold thread Barb are as follows:
The pond is raised and train in a, selection, and water level remains on 1.0-1.2m, and water temperature 16-21 ℃, dissolved oxygen in water 6.5-8.0mg/L; Hide pond face 2/3 shading with shading net, and escape with the interception of 40 order screen window nets is anti-at delivery port;
B, feed are selected and the mode of throwing something and feeding:
Laundering period: in the laundering period at initial stage of being transplanted to the pond artificial domestication, throw something and feed aquatic insect and Limnodrilus hoffmeisteri; Feeding volume is 2.5% of a fish body gross weight, and the time of throwing something and feeding is 7 days;
Change the food phase: select for use the higher mash feed of protein content to mix 10% fish meal as bait, proportioning is 9: 1, and feeding volume is 2.5% of a fish body gross weight, and the time of throwing something and feeding is 15 days;
The typing phase: selecting diameter for use is 1.0-2.0mm, the granular bait for fish of crude protein content 34%; Threw something and fed in 1st 3 times, the time is 9:00,17:00 and 21:00, and a day feeding volume is 3% of a body weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010101143894A CN101779605B (en) | 2010-02-26 | 2010-02-26 | Artificial insemination method of Sinocyclocheilus tingi |
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| CN2010101143894A CN101779605B (en) | 2010-02-26 | 2010-02-26 | Artificial insemination method of Sinocyclocheilus tingi |
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| CN101779605A true CN101779605A (en) | 2010-07-21 |
| CN101779605B CN101779605B (en) | 2012-05-30 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102893935A (en) * | 2012-11-02 | 2013-01-30 | 毕节市水产技术推广站 | Method for domesticating and raising percocypris pingi pingi |
| CN103651221A (en) * | 2013-12-30 | 2014-03-26 | 江川县水产技术推广站 | Domestication management technology of barbodes fuxianhuensis |
| CN103651220A (en) * | 2013-12-30 | 2014-03-26 | 玉溪市水产工作站（玉溪市水生动物防疫检疫站云南省渔业船舶检验局玉溪检验处） | Barbodes fuxianensis artificial propagation technology |
| CN108575939A (en) * | 2018-02-25 | 2018-09-28 | 宁波市成大机械研究所 | A kind of small medicine for the black carp that is specifically used to fish |
| CN110226539A (en) * | 2019-07-17 | 2019-09-13 | 会泽县水产工作站 | A kind of chartering cost technology of Huize gold thread Barb |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101554144A (en) * | 2009-05-22 | 2009-10-14 | 中国科学院昆明动物研究所 | Artificial fertilization and incubation method of sinocyclocheilus grahami |
-
2010
- 2010-02-26 CN CN2010101143894A patent/CN101779605B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101554144A (en) * | 2009-05-22 | 2009-10-14 | 中国科学院昆明动物研究所 | Artificial fertilization and incubation method of sinocyclocheilus grahami |
Non-Patent Citations (5)
| Title |
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| 《云南农业科技》 20091231 李维贤等 金线鱼繁殖方法 55-57 1 , 第1期 2 * |
| 《动物学研究》 20070630 杨君兴等 云南滇池濒危特有种滇池金线鲃人工繁殖初报 329-331 1 第28卷, 第3期 2 * |
| 《动物学研究》 20090831 潘晓赋等 抚仙金线鲃人工繁殖与鱼苗培育技术 463-467 1 第30卷, 第4期 2 * |
| 《水生态学杂志》 20090731 严晖等 尖头金线鲃人工繁殖初步研究 143-145 1 第2卷, 第4期 2 * |
| 《水生态学杂志》 20100131 严晖等 滇池金线鲃全人工繁殖研究 138-141 1 第3卷, 第1期 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102893935A (en) * | 2012-11-02 | 2013-01-30 | 毕节市水产技术推广站 | Method for domesticating and raising percocypris pingi pingi |
| CN103651221A (en) * | 2013-12-30 | 2014-03-26 | 江川县水产技术推广站 | Domestication management technology of barbodes fuxianhuensis |
| CN103651220A (en) * | 2013-12-30 | 2014-03-26 | 玉溪市水产工作站（玉溪市水生动物防疫检疫站云南省渔业船舶检验局玉溪检验处） | Barbodes fuxianensis artificial propagation technology |
| CN108575939A (en) * | 2018-02-25 | 2018-09-28 | 宁波市成大机械研究所 | A kind of small medicine for the black carp that is specifically used to fish |
| CN110226539A (en) * | 2019-07-17 | 2019-09-13 | 会泽县水产工作站 | A kind of chartering cost technology of Huize gold thread Barb |
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| Publication number | Publication date |
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| CN101779605B (en) | 2012-05-30 |
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