CN103478042A - Artificial propagation method of Gymnocypriseckloni - Google Patents
Artificial propagation method of Gymnocypriseckloni Download PDFInfo
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- CN103478042A CN103478042A CN201310449804.5A CN201310449804A CN103478042A CN 103478042 A CN103478042 A CN 103478042A CN 201310449804 A CN201310449804 A CN 201310449804A CN 103478042 A CN103478042 A CN 103478042A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to an artificial propagation method of Gymnocypriseckloni. The method includes the steps of firstly, selecting parent fish and inducing spawning; secondly, using net cage hatching or flowing water hatching. The artificial propagation method is high in fertility rate, hatchability and survival rate, the fertility rate, the hatchability and the survival rate can reach above 80%, and Gymnocypriseckloni resources in the upstream of the Yellow River can be recovered effectively.
Description
technical field:
Of the present invention relevant with the Gymnocypris eckloni (Herzenstein) artificial fecundation method.
background technology:
Gymnocypris eckloni (Herzenstein) (
gymnocypris eckloniherzensten) belong to Cypriniformes, Cyprinidae, Gymnocypris.Be commonly called as: large face fish, large mouth flower fish, large mouth fish.Gymnocypris eckloni (Herzenstein) is mainly perched among broad valley river course, plateau.Individuality is larger.Be distributed in the how Qihe water system in the upper reaches of the Yellow River and the Caidamu Basin, the contiguous water system in Sichuan, Gansu, Qinghai and the Yellow River has distribution, is Important Economic fish in source on the Yellow River.Belong to ominivore-fish, the feeding habits scope is wide, inhabits the middle level of water, 2～3 kilograms of body weight maximums, even 5～6 kilograms.Poor growth, meat is better, is a kind of tuna fisheries that there is DEVELOPMENT PROSPECT in Qinghai.Its meat greasiness, taste is fresh and tender, being rich in nutrition, and roe is poisonous, can not eat.The flesh of fish, also can be drying, stew in soy sauce, smoke and make canned food except eating raw, is convenient to hide for a long time and find a good sale in.Because cruel fishing is excessively captured and the destruction of ecotope, result causes resource significantly to descend, and is put into " Chinese species Red List ", and grade in imminent danger be easily endanger (V).In order to save this resource, be not exterminated, taking artificial propagation is a kind of effective way, but, up to now still without any the message to the Gymnocypris eckloni (Herzenstein) successful reproduction, also without any document, shows to have the people once to carry out similarly work.
summary of the invention:
The objective of the invention is for a kind of fertilization rate, incubation rate, artificial propagation Gymnocypris eckloni (Herzenstein) method that survival rate is high are provided.
The object of the present invention is achieved like this:
(1) selection of parent population with hasten parturition:
A. the selection of parent population: with the physique stalwartness, anosis without wound, the sexually matured parent population of 0.5～1.5kg is main, and the belly of raun expands softness, and the ovary profile is obvious, and gonopore swelling is slightly micro-red; Milter presses belly to have seminal fluid to flow out with have gentle hands, after water breakthrough, scatters immediately, is creamy white;
B. the selection of oxytocic drug and dosage: the good raun for maturity, water temperature can be hastened parturition in the time of 7～12 ℃, and oxytocic drug and consumption are: LRH-A
2(short Luteinizing hormone releasing hormone analog) every kg body weight is 3～6ug; The HCG(human chorionic gonadtropin) every kg body weight is 1000～1500IU, minute 2 injections, and injection is for to inject remaining all dosage for the 1/8～1/6,2nd pin of accumulated dose for the first time, and milter adopts disposable injection, and dosage is 1/2 of raun accumulated dose;
C. dry method of artificial fertilization: by after ripe raun body surface water pole, squeeze the ovum engagement, clamp-on seminal fluid simultaneously, stir 2～3 min, sperm is fully contacted with ovum, now add again at least 500ml of the clear water identical with Spawning water, activate sperm and ovum, after continuing to stir at least 1min, add the clean unnecessary seminal fluid of at least 500ml clear water rinsing and foreign material, pour the incubator box hatching into;
(2) hatching: adopt net cage hatching or Lotic hatching:
A. adopt the net cage hatching, net cage is placed sleep, and Spawning water is Natural Water, dissolved oxygen >=5mg/L, and 7～15 ℃ of hatching water temperatures, the pH value is 7.2～8.0, places 1 layer of ovum in case;
B. adopt Lotic hatching, should select in time bad ovum in hatching, the ovum of not inseminating, dead ovum, incubator box will be avoided illumination;
C. maintain aforementioned hatching process until the absorption of prelarva yolk sac is complete, the yolk water that yolk sac should be thrown something and fed after disappearing after filtering is until put down trip, 15～20 ℃ of the cultivation water temperatures of growing seedlings.
The artificial propagation method fertilization rate of the present invention, incubation rate, survival rate are high, and fertilization rate can reach more than 80%, and incubation rate and survival rate can reach more than 80%, and efficient recovery the upper reaches of the Yellow River Gymnocypris eckloni (Herzenstein) resource can be arranged.
embodiment:
The present embodiment Gymnocypris eckloni (Herzenstein) artificial fecundation method comprises the following steps.
(1) selection of parent population with hasten parturition:
A. the selection of parent population: with the physique stalwartness, anosis without wound, the sexually matured parent population of 0.5～1.5kg is main.The belly of raun expands softness, and the ovary profile is obvious, and gonopore swelling is slightly micro-red; Milter presses belly to have seminal fluid to flow out with have gentle hands, after water breakthrough, scatters immediately, is creamy white;
B. the selection of oxytocic drug and dosage: the good raun for maturity, water temperature can be hastened parturition in the time of 7～12 ℃, and oxytocic drug and consumption are: LRH-A
2every kg body weight is 4.5ug; The every kg body weight of HCG is 1250IU, mixes use, minute 2 injections, and injection is for to inject remaining all dosage for 1/7, the 2 pin of accumulated dose for the first time, and milter adopts disposable injection, and dosage is 1/2 of raun accumulated dose;
C. dry method of artificial fertilization: by after ripe raun body surface water pole, squeeze the ovum engagement, clamp-on seminal fluid simultaneously, stir 2～3 min with feather, sperm is fully contacted with ovum, now add again the clear water 500mL identical with Spawning water, activate sperm and ovum, after continuing to stir 1min, add the clean unnecessary seminal fluid of 500mL clear water rinsing and foreign material, pour the incubator box hatching into.
(2) hatching:
A. adopt the net cage hatching, net cage is placed sleep, and Spawning water is Natural Water, dissolved oxygen >=5mg/L, and 9～13 ℃ of hatching water temperatures, the pH value is 7.5, places one deck ovum in case;
B. adopt Lotic hatching, should select in time bad ovum in hatching, the ovum of not inseminating, dead ovum, incubator box will be avoided illumination;
C. maintain aforementioned hatching process until the absorption of prelarva yolk sac is complete, the yolk water that yolk sac should be thrown something and fed after disappearing after filtering is until put down trip, the cultivation 18 ℃ of left and right of water temperature of growing seedlings.
The various embodiments described above are that foregoing of the present invention is further described, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to above-described embodiment.All technology realized based on foregoing all belong to scope of the present invention.
Claims (1)
1. Gymnocypris eckloni (Herzenstein) artificial fecundation method, the method comprises the following steps:
(1) selection of parent population with hasten parturition:
A. the selection of parent population: with the physique stalwartness, anosis without wound, the sexually matured parent population of 0.5～1.5kg is main, and the belly of raun expands softness, and the ovary profile is obvious, and gonopore swelling is slightly micro-red; Milter presses belly to have seminal fluid to flow out with have gentle hands, after water breakthrough, scatters immediately, is creamy white;
B. the selection of oxytocic drug and dosage: the good raun for maturity, water temperature can be hastened parturition in the time of 7～12 ℃, and oxytocic drug and consumption are: LRH-A
2every kg body weight is 3～6ug; The every kg body weight of HCG is 1000～1500IU, minute 2 injections, and injection is for to inject remaining all dosage for the 1/8～1/6,2nd pin of accumulated dose for the first time, and milter adopts disposable injection, and dosage is 1/2 of raun accumulated dose;
C. dry method of artificial fertilization: after ripe raun body surface water is dried, squeeze the ovum engagement, clamp-on seminal fluid simultaneously, stir 2～3min, sperm is fully contacted with ovum, now add again at least 500ml of the clear water identical with Spawning water, activate sperm and ovum, after continuing to stir at least 1min, add the clean unnecessary seminal fluid of at least 500mL clear water rinsing and foreign material, pour the incubator box hatching into;
(2) hatching: adopt net cage hatching or Lotic hatching:
A. adopt the net cage hatching, net cage is placed sleep, and Spawning water is Natural Water, dissolved oxygen >=5mg/L, and 7～15 ℃ of hatching water temperatures, the pH value is 7.2～8.0, places 1 layer of ovum in case;
B. adopt Lotic hatching, should select in time bad ovum in hatching, the ovum of not inseminating, dead ovum, incubator box will be avoided illumination;
C. maintain aforementioned hatching process until the absorption of prelarva yolk sac is complete, the yolk water that yolk sac should be thrown something and fed after disappearing after filtering is until put down trip, 15～20 ℃ of the cultivation water temperatures of growing seedlings.
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| CN201310449804.5A CN103478042B (en) | 2013-09-29 | 2013-09-29 | Artificial propagation method of Gymnocypriseckloni |
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| CN201310449804.5A CN103478042B (en) | 2013-09-29 | 2013-09-29 | Artificial propagation method of Gymnocypriseckloni |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012435A (en) * | 2014-06-13 | 2014-09-03 | 临沂大学 | Efficient breeding method for Rhodeus sinensis |
| CN104396813A (en) * | 2014-10-31 | 2015-03-11 | 井陉县鱼泉冷水鱼开发有限公司 | Gymnocypris przewalskii full artificial breeding method |
| CN104585094A (en) * | 2015-01-16 | 2015-05-06 | 苏州市水产研究所有限公司 | Method for breeding Xenocypris argentea Gunther artificially |
| CN104719218A (en) * | 2015-04-10 | 2015-06-24 | 宁国市金东坊农业开发有限公司 | Tor-brevifilis full-artificial early propagation method |
| CN109511579A (en) * | 2018-10-17 | 2019-03-26 | 华能西藏雅鲁藏布江水电开发投资有限公司藏木水电厂 | The artificial propagation techniques of sharp naked carp |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1085686A (en) * | 1965-08-20 | 1967-10-04 | Olav Clausen Vik | Hatching and breeding of fish |
-
2013
- 2013-09-29 CN CN201310449804.5A patent/CN103478042B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1085686A (en) * | 1965-08-20 | 1967-10-04 | Olav Clausen Vik | Hatching and breeding of fish |
Non-Patent Citations (4)
| Title |
|---|
| 史建全等: "青海湖裸鲤人工繁殖及鱼苗培育技术的研究", 《淡水渔业》 * |
| 曾海红: "青海湖裸鲤繁养技术", 《河北渔业》 * |
| 谢德钦: "《高效益池塘养鱼技术》", 31 July 1998, 广西科学技术出版社 * |
| 陈大庆等: "《青海湖裸鲤研究与保护》", 31 May 2011, 科学出版社 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012435A (en) * | 2014-06-13 | 2014-09-03 | 临沂大学 | Efficient breeding method for Rhodeus sinensis |
| CN104012435B (en) * | 2014-06-13 | 2015-12-30 | 临沂大学 | China Bitterling Welfare (Rhodeus sinensis) efficient propagation method |
| CN104396813A (en) * | 2014-10-31 | 2015-03-11 | 井陉县鱼泉冷水鱼开发有限公司 | Gymnocypris przewalskii full artificial breeding method |
| CN104585094A (en) * | 2015-01-16 | 2015-05-06 | 苏州市水产研究所有限公司 | Method for breeding Xenocypris argentea Gunther artificially |
| CN104719218A (en) * | 2015-04-10 | 2015-06-24 | 宁国市金东坊农业开发有限公司 | Tor-brevifilis full-artificial early propagation method |
| CN109511579A (en) * | 2018-10-17 | 2019-03-26 | 华能西藏雅鲁藏布江水电开发投资有限公司藏木水电厂 | The artificial propagation techniques of sharp naked carp |
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