CN104719218A - Tor-brevifilis full-artificial early propagation method - Google Patents
Tor-brevifilis full-artificial early propagation method Download PDFInfo
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- CN104719218A CN104719218A CN201510168899.2A CN201510168899A CN104719218A CN 104719218 A CN104719218 A CN 104719218A CN 201510168899 A CN201510168899 A CN 201510168899A CN 104719218 A CN104719218 A CN 104719218A
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 241000703814 Folifer brevifilis Species 0.000 title abstract 17
- 241000251468 Actinopterygii Species 0.000 claims abstract description 119
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 69
- 238000002347 injection Methods 0.000 claims abstract description 37
- 239000007924 injection Substances 0.000 claims abstract description 37
- 238000009395 breeding Methods 0.000 claims abstract description 21
- 230000001488 breeding effect Effects 0.000 claims abstract description 21
- 230000009027 insemination Effects 0.000 claims abstract description 16
- 210000000582 semen Anatomy 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 14
- 230000016087 ovulation Effects 0.000 claims description 17
- 230000006698 induction Effects 0.000 claims description 16
- 241000252233 Cyprinus carpio Species 0.000 claims description 11
- 241000404975 Synchiropus splendidus Species 0.000 claims description 11
- 230000037396 body weight Effects 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 10
- 210000004681 ovum Anatomy 0.000 claims description 7
- 230000012447 hatching Effects 0.000 claims description 6
- 206010013786 Dry skin Diseases 0.000 claims description 5
- 244000144987 brood Species 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000010802 sludge Substances 0.000 claims description 5
- 102000002322 Egg Proteins Human genes 0.000 claims description 4
- 108010000912 Egg Proteins Proteins 0.000 claims description 4
- 239000000203 mixture Substances 0.000 abstract description 3
- 230000017448 oviposition Effects 0.000 abstract description 3
- 235000013601 eggs Nutrition 0.000 abstract 2
- 230000035558 fertility Effects 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 210000001550 testis Anatomy 0.000 abstract 1
- 230000004720 fertilization Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 210000004246 corpus luteum Anatomy 0.000 description 1
- 230000001076 estrogenic effect Effects 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 239000003163 gonadal steroid hormone Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 210000002394 ovarian follicle Anatomy 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a tor-brevifilis full-artificial early propagation method. The tor-brevifilis full-artificial early propagation method includes the following steps: A, selecting a parent tor brevifilis pond; B, selecting parent tor brevifilises; C, breeding the parent tor brevifilises; D, adjusting the temperature of water, wherein the temperature of the water in the parent tor brevifilis pond rises through a hot water source from February and rises to 25 DEG C in mid-March, and then the parent tor brevifilis pond is washed through cold water once every five days; E, promoting egg laying, wherein in the beginning of April, the female tor brevifilises are injected with LRH-A2 for the first time and injected with LRH-A2 and HCG for the second time after six hours, the male tor brevifilises are injected with fish LRH-A2 and HCG, and after injection is completed, the female tor brevifilises and the male tor brevifilises are paired to be placed into an egg laying pond; F, carrying out artificial insemination, wherein after the mature parent tor brevifilises are caught and the surfaces are dried, female tor brevifilis ova are extruded into a dry utensil; meanwhile, seminal fluid of the male tor brevifilises is extruded into the utensil, stirring is carried out, testis tissues and dirt are sucked away, then fertilized eggs are injected into yellow mud water, continuous stirring is carried out, the mixture is poured into a dense net, and the fertilized eggs are filtered out, rinsed in water and placed into an incubation pond to be incubated. By means of the tor-brevifilis full-artificial early propagation method, the breeding period of the tor brevifilises is early, and the fertility rate can be 85%.
Description
Technical field
The present invention relates to fish culture technical field, particularly relate to a kind of lobe knot fish total man work and do sth. in advance propagation method.
Background technology
Lobe knot fish is a kind of medium-sized economic freshwater fish, and be distributed on the south ChangJiang River, Pearl River system, be the economic fish that producing region is common, it is Fresh & Tender in Texture, be people institute eating, and fat content is high, and growth is very fast, has higher Fishery value.General 3 age in winter fish begin to reach sexual maturity, but the repoductive time of this fish is comparatively slow, and the egg-laying period, between the 6-7 month, impact vegetative period then, extends the culture-cycle of forming listing, and fertilization rate in natural breeding process is not high.
Summary of the invention
Based on the technical problem that background technology exists, the present invention proposes a kind of lobe knot fish total man work and do sth. in advance propagation method, take the mode of hot water temperature raising and regular cold water stimulating in early days, make lobe tie fish and done sth. in advance 1 month breeding period, adopt artificial dry method to inseminate, its fertilization rate can reach 85% simultaneously.
A kind of lobe knot fish total man work that the present invention proposes does sth. in advance propagation method, comprises the steps:
A, selection parent fish pond: parent fish pond four limit is straight, and the area of parent fish pond is 2668-3335m
2, smooth non sludge at the bottom of pond, depth of water 1.5-2.0m;
B, selection parent population: choose the body surface anosis knot of the lobe without wound fish as parent population, wherein the milter age is 2-3 age, and male fish length is more than 22cm, and milter body weight is more than 240g, and the raun age is 3-4 age, and female fish length is more than 30cm, and raun body weight is more than 680g;
C, put parent population in a suitable place to breed: lobe being tied fish parent fish by sex ration is that 1:1-2 carries out pairing and puts in a suitable place to breed in parent fish pond, and the breeding density of lobe knot fish parent fish is 20-25g/m
2, then put mandarin fish and variegated carp in a suitable place to breed, wherein the breeding density of mandarin fish is 0.011-0.015 tail/m
2, the breeding density of variegated carp is 0.07-0.08 tail/m
2;
D, adjustment water temperature: utilize thermal water source from February, raise the water temperature of parent fish pond, be increased to 25 DEG C to water temperature during mid-March, then rushed pond once every 5 days with cold water, each bath 2-3 hour;
E, to promote production: carry out injection at the beginning of 4 months and promote production, wherein raun divides 2 injections, first time injection fish ovulation induction element No. 2 10 μ g/kg, carry out second time injection after 6 hours, injection volume is fish ovulation induction element No. 2 15 μ g/kg and human chorionic gonadtropin 300-500IU/kg; Milter injection fish ovulation induction element No. 2 12.5 μ g/kg and human chorionic gonadtropin 150-250IU/kg; After injection by sex ration be 1:1-1.5 pairing put into spawning pond;
F, artificial insemination: when finding that in spawning pond, milter starts to chase raun, can artificial insemination be carried out; Mature brood fish has been caught put on the skin dry after, female fish egg is clamp-oned in dry vessel, clamp-on the seminal fluid of milter simultaneously, then stir 2-3min, sucking-off spermary tissue and dirt, then fertilized egg is injected yellow mud water, then 8-12min is stirred clockwise or counterclockwise, pour in close net and leach fertilized egg, in water after rinsing 1-2 time, put into hatchery hatching.
Parent fish pond should be had abundant water resources, and water quality is pure and fresh, pollution-free, and arrange, pour water conveniently, near spawning pond, soil property water conservation, fertilizer-preserving ability are strong, and physical features is smooth, and illumination condition is good.
Put in a suitable place to breed in process, add mandarin fish and variegated carp and can control growing of wild fishes and macrozooplankton.
In artificial insemination procedures, when female fish egg and milter seminal fluid mix and blend, dry feather is adopted to stir gently; Need stir to a direction when fertilized egg is stirred after injecting yellow mud water, ensure that fertilized egg does not pile up agglomerating, caking in mud; Fertilized egg stirs 8-12min after injecting yellow mud water should make the viscosity of fertilized egg take off completely.
Preferably, regulate in water temperature process, utilize thermal water source from February, raise the water temperature of parent fish pond by the average heating rate of 0.5 DEG C/d, avoid lobe knot fish parent fish and produce stress reaction.
Preferably, regulate in water temperature process, cold water to be temperature the be water of 5-10 DEG C.
Preferably, in artificial insemination procedures, every 2-5 ten thousand ovum instillation 2-3 drip seminal fluid.
Preferably, in artificial insemination procedures, the aperture of close net is 0.45-0.55mm.
The present invention, by adopting the mode of hot water temperature raising and regular cold water stimulating, impels lobe to tie fish gonad development, improves the concentration of sex hormone in lobe knot fish body; Adopt injection fish ovulation induction plain No. 2 and human chorionic gonadtropin again, impel the generation of milter sperm and maturation further, impel raun ovarian follicle to secrete oestrogenic hormone, follicle maturity, ovulation and corpus luteum are generated, thus greatly impel lobe knot fish to shift to an earlier date breeding period; Artificial dry method is adopted to inseminate, ovum and seminal fluid are directly mixed contact, again by controlling the ratio of ovum and seminal fluid, substantially increase the fertilization rate of lobe knot fish, stir by fertilized egg being injected yellow mud water again, the viscosity of fertilized egg is taken off completely, in hatching process, avoids the situation that fertilized egg sticking to each other causes local density excessive.
Embodiment
Below, by specific embodiment, technical scheme of the present invention is described in detail.
Embodiment 1
A kind of lobe knot fish total man work that the present invention proposes does sth. in advance propagation method, comprises the steps:
A, selection parent fish pond: parent fish pond four limit is straight, and the area of parent fish pond is 2668m
2, smooth non sludge at the bottom of pond, depth of water 2.0m;
B, selection parent population: choose the body surface anosis knot of the lobe without wound fish as parent population, wherein the milter age is 2-3 age, and male fish length is more than 22cm, and milter body weight is more than 240g, and the raun age is 3-4 age, and female fish length is more than 30cm, and raun body weight is more than 680g;
C, put parent population in a suitable place to breed: lobe being tied fish parent fish by sex ration is that 1:1 carries out pairing and puts in a suitable place to breed in parent fish pond, and the breeding density of lobe knot fish parent fish is 25g/m
2, then put mandarin fish and variegated carp in a suitable place to breed, wherein the breeding density of mandarin fish is 0.011 tail/m
2, the breeding density of variegated carp is 0.08 tail/m
2;
D, adjustment water temperature: utilize thermal water source from February, raise the water temperature of parent fish pond by the average heating rate of 0.5 DEG C/d, be increased to 25 DEG C to water temperature during mid-March, then rushed pond once every 5 days with the cold water that temperature is 5 DEG C, each bath 2 hours;
E, to promote production: carry out injection at the beginning of 4 months and promote production, wherein raun divides 2 injections, first time injection fish ovulation induction element No. 2 10 μ g/kg, carry out second time injection after 6 hours, injection volume is fish ovulation induction element No. 2 15 μ g/kg and human chorionic gonadtropin 500IU/kg; Milter injection fish ovulation induction element No. 2 12.5 μ g/kg and human chorionic gonadtropin 150IU/kg; After injection by sex ration be 1:1.5 pairing put into spawning pond;
F, artificial insemination: when finding that in spawning pond, milter starts to chase raun, can artificial insemination be carried out; Mature brood fish has been caught put on the skin dry after, female fish egg is clamp-oned in dry vessel, clamp-on the seminal fluid of milter, every 20,000 ovums instill 2 seminal fluid, then stir 2min simultaneously, sucking-off spermary tissue and dirt, then fertilized egg is injected yellow mud water, then stir 12min clockwise or counterclockwise, pouring aperture into is leach fertilized egg in the close net of 0.45-0.55mm, in water after rinsing 1 time, put into hatchery hatching.
Embodiment 2
A kind of lobe knot fish total man work that the present invention proposes does sth. in advance propagation method, comprises the steps:
A, selection parent fish pond: parent fish pond four limit is straight, and the area of parent fish pond is 2668-3335m
2, smooth non sludge at the bottom of pond, depth of water 1.5-2.0m;
B, selection parent population: choose the body surface anosis knot of the lobe without wound fish as parent population, wherein the milter age is 2-3 age, and male fish length is more than 22cm, and milter body weight is more than 240g, and the raun age is 3-4 age, and female fish length is more than 30cm, and raun body weight is more than 680g;
C, put parent population in a suitable place to breed: lobe being tied fish parent fish by sex ration is that 1:1-2 carries out pairing and puts in a suitable place to breed in parent fish pond, and the breeding density of lobe knot fish parent fish is 20-25g/m
2, then put mandarin fish and variegated carp in a suitable place to breed, wherein the breeding density of mandarin fish is 0.011-0.015 tail/m
2, the breeding density of variegated carp is 0.07-0.08 tail/m
2;
D, adjustment water temperature: utilize thermal water source from February, raise the water temperature of parent fish pond by the average heating rate of 0.5 DEG C/d, be increased to 25 DEG C to water temperature during mid-March, then rushed pond once every 5 days with the cold water that temperature is 8 DEG C, each bath 2-3 hour;
E, to promote production: carry out injection at the beginning of 4 months and promote production, wherein raun divides 2 injections, first time injection fish ovulation induction element No. 2 10 μ g/kg, carry out second time injection after 6 hours, injection volume is fish ovulation induction element No. 2 15 μ g/kg and human chorionic gonadtropin 300-500IU/kg; Milter injection fish ovulation induction element No. 2 12.5 μ g/kg and human chorionic gonadtropin 150-250IU/kg; After injection by sex ration be 1:1-1.5 pairing put into spawning pond;
F, artificial insemination: when finding that in spawning pond, milter starts to chase raun, can artificial insemination be carried out; Mature brood fish has been caught put on the skin dry after, female fish egg is clamp-oned in dry vessel, clamp-on the seminal fluid of milter, every 30,000 ovums instill 2.5 seminal fluid, then stir 2-3min simultaneously, sucking-off spermary tissue and dirt, then fertilized egg is injected yellow mud water, then stir 8-12min clockwise or counterclockwise, pouring aperture into is leach fertilized egg in the close net of 0.45-0.55mm, in water after rinsing 1-2 time, put into hatchery hatching.
Embodiment 3
A kind of lobe knot fish total man work that the present invention proposes does sth. in advance propagation method, comprises the steps:
A, selection parent fish pond: parent fish pond four limit is straight, and the area of parent fish pond is 3335m
2, smooth non sludge at the bottom of pond, depth of water 1.5m;
B, selection parent population: choose the body surface anosis knot of the lobe without wound fish as parent population, wherein the milter age is 2-3 age, and male fish length is more than 22cm, and milter body weight is more than 240g, and the raun age is 3-4 age, and female fish length is more than 30cm, and raun body weight is more than 680g;
C, put parent population in a suitable place to breed: lobe being tied fish parent fish by sex ration is that 1:2 carries out pairing and puts in a suitable place to breed in parent fish pond, and the breeding density of lobe knot fish parent fish is 20g/m
2, then put mandarin fish and variegated carp in a suitable place to breed, wherein the breeding density of mandarin fish is 0.015 tail/m
2, the breeding density of variegated carp is 0.07 tail/m
2;
D, adjustment water temperature: utilize thermal water source from February, raise the water temperature of parent fish pond by the average heating rate of 0.5 DEG C/d, be increased to 25 DEG C to water temperature during mid-March, then rushed pond once every 5 days with the cold water that temperature is 10 DEG C, each bath 3 hours;
E, to promote production: carry out injection at the beginning of 4 months and promote production, wherein raun divides 2 injections, first time injection fish ovulation induction element No. 2 10 μ g/kg, carry out second time injection after 6 hours, injection volume is fish ovulation induction element No. 2 15 μ g/kg and human chorionic gonadtropin 300IU/kg; Milter injection fish ovulation induction element No. 2 12.5 μ g/kg and human chorionic gonadtropin 250IU/kg; After injection by sex ration be 1:1 pairing put into spawning pond;
F, artificial insemination: when finding that in spawning pond, milter starts to chase raun, can artificial insemination be carried out; Mature brood fish has been caught put on the skin dry after, female fish egg is clamp-oned in dry vessel, clamp-on the seminal fluid of milter, every 50,000 ovums instill 3 seminal fluid, then stir 3min simultaneously, sucking-off spermary tissue and dirt, then fertilized egg is injected yellow mud water, then stir 8min clockwise or counterclockwise, pouring aperture into is leach fertilized egg in the close net of 0.45-0.55mm, in water after rinsing 2 times, put into hatchery hatching.
Adopt the method for embodiment 2, make lobe tie fish and done sth. in advance 1 month breeding period, and the fertilization rate reached 85% of lobe knot fish.
The above; be only the present invention's preferably embodiment; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.
Claims (5)
1. lobe knot fish total man work does sth. in advance a propagation method, it is characterized in that, comprises the steps:
A, selection parent fish pond: parent fish pond four limit is straight, and the area of parent fish pond is 2668-3335m
2, smooth non sludge at the bottom of pond, depth of water 1.5-2.0m;
B, selection parent population: choose the body surface anosis knot of the lobe without wound fish as parent population, wherein the milter age is 2-3 age, and male fish length is more than 22cm, and milter body weight is more than 240g, and the raun age is 3-4 age, and female fish length is more than 30cm, and raun body weight is more than 680g;
C, put parent population in a suitable place to breed: lobe being tied fish parent fish by sex ration is that 1:1-2 carries out pairing and puts in a suitable place to breed in parent fish pond, and the breeding density of lobe knot fish parent fish is 20-25g/m
2, then put mandarin fish and variegated carp in a suitable place to breed, wherein the breeding density of mandarin fish is 0.011-0.015 tail/m
2, the breeding density of variegated carp is 0.07-0.08 tail/m
2;
D, adjustment water temperature: utilize thermal water source from February, raise the water temperature of parent fish pond, be increased to 25 DEG C to water temperature during mid-March, then rushed pond once every 5 days with cold water, each bath 2-3 hour;
E, to promote production: carry out injection at the beginning of 4 months and promote production, wherein raun divides 2 injections, first time injection fish ovulation induction element No. 2 10 μ g/kg, carry out second time injection after 6 hours, injection volume is fish ovulation induction element No. 2 15 μ g/kg and human chorionic gonadtropin 300-500IU/kg; Milter injection fish ovulation induction element No. 2 12.5 μ g/kg and human chorionic gonadtropin 150-250IU/kg; After injection by sex ration be 1:1-1.5 pairing put into spawning pond;
F, artificial insemination: when finding that in spawning pond, milter starts to chase raun, can artificial insemination be carried out; Mature brood fish has been caught put on the skin dry after, female fish egg is clamp-oned in dry vessel, clamp-on the seminal fluid of milter simultaneously, then stir 2-3min, sucking-off spermary tissue and dirt, then fertilized egg is injected yellow mud water, then 8-12min is stirred clockwise or counterclockwise, pour in close net and leach fertilized egg, in water after rinsing 1-2 time, put into hatchery hatching.
2. lobe knot fish total man work does sth. in advance propagation method according to claim 1, it is characterized in that, regulates in water temperature process, utilizes thermal water source from February, raise the water temperature of parent fish pond by the average heating rate of 0.5 DEG C/d.
3. according to claim 1 or 2, lobe knot fish total man work does sth. in advance propagation method, it is characterized in that, regulates in water temperature process, cold water to be temperature the be water of 5-10 DEG C.
4. according to any one of claim 1-3, lobe knot fish total man work does sth. in advance propagation method, it is characterized in that, in artificial insemination procedures, every 2-5 ten thousand ovum instillation 2-3 drip seminal fluid.
5. according to any one of claim 1-4, lobe knot fish total man work does sth. in advance propagation method, and it is characterized in that, in artificial insemination procedures, the aperture of close net is 0.45-0.55mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510168899.2A CN104719218B (en) | 2015-04-10 | 2015-04-10 | A kind of lobe knot fish manually does sth. in advance propagation method entirely |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510168899.2A CN104719218B (en) | 2015-04-10 | 2015-04-10 | A kind of lobe knot fish manually does sth. in advance propagation method entirely |
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| CN104719218B CN104719218B (en) | 2017-03-15 |
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| CN103461254A (en) * | 2013-09-29 | 2013-12-25 | 四川省农业科学院水产研究所 | Scale artificial propagation method for procypris rabaudi |
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2015
- 2015-04-10 CN CN201510168899.2A patent/CN104719218B/en not_active Expired - Fee Related
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| CN103461254A (en) * | 2013-09-29 | 2013-12-25 | 四川省农业科学院水产研究所 | Scale artificial propagation method for procypris rabaudi |
| CN103478042A (en) * | 2013-09-29 | 2014-01-01 | 四川省农业科学院水产研究所 | Artificial propagation method of Gymnocypriseckloni |
| CN104161000A (en) * | 2014-08-28 | 2014-11-26 | 沈阳华泰渔业有限公司 | Hybrid carp and pengze crucian carp hybrid breeding method |
| CN104304103A (en) * | 2014-09-17 | 2015-01-28 | 丹东市水产技术推广总站 | Seedling breeding method for early breeding and cultivating large-size hemibarbus labeo |
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