WO2023184693A1

WO2023184693A1 - Maturation promotion method for cultivating mactra antiquata parent shellfishes by using shrimp pond algae

Info

Publication number: WO2023184693A1
Application number: PCT/CN2022/095873
Authority: WO
Inventors: 罗杰; 王中铎; 郭昱嵩
Original assignee: 广东海洋大学
Priority date: 2022-03-31
Filing date: 2022-05-30
Publication date: 2023-10-05
Also published as: LU502637B1; CN114600806B; CN114600806A

Abstract

A maturation promotion method for cultivating Mactra antiquata parent shellfishes by using shrimp pond algae. The method comprises the following steps: putting Mactra antiquata parent shellfishes in a culture pond for maturation promotion cultivation, and adding shrimp pond water into the culture pond to the height of a water discharge pipe; and performing daily management, specifically comprising: every morning, changing half of the water and then adding the shrimp pond water up to the original water depth; every afternoon, extracting the shrimp pond water, adding same to the culture pond and enabling convection of the pond water for a certain period of time; and regularly cleaning a surface sand layer.

Description

A maturation-promoting method for cultivating Xishi tongue oysters using shrimp pond algae

Technical field

The present invention relates to the technical field of Xi Shi tongue cultivation, and in particular to a maturation-promoting method for cultivating Xi Shi tongue oysters using shrimp pond algae.

Background technique

Xishi tongue is a large bivalve shell that lives in shallow seas. It feeds on single-cell algae and organic debris in the water. It is commonly known as royal snail. Xishi tongue is large in size, has a high meat content, is tender and delicious, and is rich in nutrients. It is a popular and valuable edible shellfish. In recent years, the demand for Shih Tzu Tongue has been increasing, and various predatory harvesting methods (such as electric trawling, rake nets, etc.) have led to a sharp decrease in production and serious damage to the resources. Therefore, it is extremely important to protect the resources of Shih Tzu. Research on artificial breeding and breeding of Shih Tzu is an effective way to solve the current resource shortage. Obtaining mature Shih Tzu oysters and inducing spawning to release sperm eggs is the key to artificial breeding. The traditional methods of promoting ripening of Xishi tongue oysters mainly include: 1) promoting ripening by feeding artificially cultivated monocystis algae; 2) promoting ripening by regulating water temperature. However, artificially cultivating Monocystis requires a lot of manpower and material resources, and the growth of algae is often greatly affected by the weather, so the supply of algae cannot be guaranteed. In addition, the artificially cultivated Monocystis is fed with a single species, and the Shih Tzu tongue after ingestion Incomplete nutrition is not conducive to the maturation of Shih Tzu tongue gonads; and regulating water temperature requires a large amount of energy, which is not in line with the production concept of low-carbon emission reduction.

Contents of the invention

Based on the above discussion, the present invention provides a maturation-promoting method for cultivating Xishi tongue oysters using shrimp pond algae. Under natural conditions, without regulating the water temperature, the shrimp pond algae and organic debris in the shrimp pond are used as bait for Xishi tongue. Realize the ripening effect of Xi Shi tongue oysters.

In order to achieve the above objects, the present invention provides the following solutions:

The present invention provides a ripening promotion method for cultivating Xishi tongue oysters using shrimp pond algae, which includes the following steps:

Put the Xishi tongue broodstock into a culture pond for maturation promotion, and add shrimp pond water to the culture pond to the height of the drainage pipe;

Daily management is as follows: change 1/2 of the water every morning, use a water pump (2-inch water pump, flow rate: 15m ³ /hour) to add shrimp pond water to the original water depth; in the afternoon, directly extract shrimp pond water and add it to the culture pond, and Allow the pool water to continue convection; clean the sand layer on the surface regularly.

Furthermore, the stocking density of Xishi tongue oysters is 15-18 individuals/m ² .

Further, the convection time is 12-18 minutes.

Further, clean the surface sand layer every 5 days.

Further, the shrimp pond water is water in a breeding pond for raising shrimps.

Further, the shrimp pond water contains green algae, diatoms, golden algae and organic debris.

The green algae, diatoms, golden algae and organic debris are all nutrients that are beneficial to the Shih Tzu tongue's feeding.

The shrimp breeding pond is specifically: a special film for breeding is laid on the bottom of the shrimp pond, and a waterwheel-type aerator is placed at the opposite corner of the pond; the shrimp pond is cleaned and disinfected, seawater filtered by coarse sand is added, and then inorganic algae cultivation is applied Nutrient salt, the dosage is compound fertilizer (total nutrients ≥ 45%, NP ₂ O ₅ -K ₂ O = 14-16-15) 12-18g/m ³ , sodium silicate (Na ₂ SiO ₃ ) 1-1.5 g/m ³ , start the aerator to fully mix the pond water with nutrient salts; cultivate for 7 to 8 days to allow the algae in the shrimp pond to grow. When the pond water reaches a certain transparency, add white shrimp seedlings. Shrimp pond management is carried out in the following way: 10-15 days after the shrimp seedlings are released, nutrients for algae growth are added. The amount of nutrients added is compound fertilizer (total nutrients ≥ 45%, NP ₂ O ₅ -K ₂ O = 14 -16-15) 4-6g/m ³ , sodium silicate 0.3-0.5g/m ³ ; in the future, due to the increase in the residual bait remaining from feeding shrimp feed and the amount of feces excreted with the growth of individual shrimp, the residual bait and The excrement of shrimp can be used as nutrients for algae; the aerator is turned on for 2 hours every morning and afternoon.

The invention discloses the following technical effects:

(1) The single-celled algae growing in shrimp ponds include green algae, diatoms and golden algae. There are many types, as well as organic debris. These are high-quality biological feeds for Xishi tongue. At the same time, the growth of algae is less affected by the weather. . Therefore, when cultivating Xishi tongue broodstock, we mainly feed shrimp pond algae and organic debris, which provide comprehensive nutrition, which can promote Xishi tongue to mature quickly and release sperm eggs, and increase the amount of sperm and eggs discharged. And after a short period of intensive cultivation after the first release of sperm and eggs, the gonads of the broodstock can mature again and release sperm and eggs.

(2) The method of the present invention for cultivating Xishi tongue oysters is simple to operate, the technology is easy to master, can save manpower and material resources, and at the same time reduces production costs.

Description of drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some of the drawings of the present invention. Embodiments, for those of ordinary skill in the art, other drawings can also be obtained based on these drawings without exerting creative efforts.

Figure 1 is a photo of a plastic frame used for cultivating Xishi tongue oysters according to the present invention;

Figure 2 is a photo of a culture tank for cultivating Xishi tongue oysters according to the present invention;

Figure 3 is a rendering of a plastic frame covering the bottom of a culture tank for cultivating Xishi tongue oysters according to the present invention.

Detailed ways

Various exemplary embodiments of the invention will now be described in detail. This detailed description should not be construed as limitations of the invention, but rather as a more detailed description of certain aspects, features and embodiments of the invention.

It should be understood that the terms used in the present invention are only used to describe particular embodiments and are not intended to limit the present invention. In addition, for numerical ranges in the present invention, it should be understood that every intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or value intermediate within a stated range and any other stated value or value intermediate within a stated range is also included within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded from the range.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only the preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and/or materials in connection with which the documents relate. In the event of conflict with any incorporated document, the contents of this specification shall prevail.

It will be apparent to those skilled in the art that various modifications and changes can be made to the specific embodiments described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to the skilled person from the description of the invention. The specification and examples of the present invention are exemplary only.

The words "includes", "includes", "has", "contains", etc. used in this article are all open terms, which mean including but not limited to.

The cultivation location of Xishi tongue of the present invention is Houhong Village, Qindou Town, Zhanjiang City, Guangdong Province.

The shrimp pond described in the embodiment of the present invention is specifically a shrimp breeding pond: the shrimp pond area is about 2000m ² , the depth of the pond is 1.5-1.7m, the bottom of the pond is covered with a special film for breeding, and a 0.75kv power generator is placed at the diagonal corner of the pond. Water wheel aerator. The shrimp pond is thoroughly cleaned and disinfected, and seawater filtered by coarse sand is added to a depth of 1.4m, and then inorganic nutrients for cultivating algae are added. The dosage is compound fertilizer 15g/m ³ and sodium silicate (Na ₂ SiO ₃ ) 1.3g/ m ³ , start the aerator to fully mix the pool water and nutrient salts. The algae in the pond will grow after 7 to 8 days of cultivation. When the pond water reaches a certain level of transparency, 90,000 white shrimp larvae with a body length of about 1cm will be released.

Shrimp pond management: 12 days after placing shrimp seedlings, add nutrients for algae growth. The additional nutrient salt dosage is 1/3 of the initial amount. In the future, due to the increase in the residual food left over from feeding the shrimp feed and the amount of feces excreted as the individual shrimp grows, both the residual food and the shrimp's excrement can be used as nutrients for algae. The aerator is turned on for 2 hours at 7:00 am and 18:00 pm every day.

In the embodiment of the present invention, the culture tank for cultivating Xishi tongue oysters is specifically: an indoor cement tank (as shown in Figure 2), with a bottom area of 20m ² and a depth of 1.0m. The auxiliary storage specifications at the bottom of the tank are 65cm long × 45cm wide × high. A 12cm plastic frame (the plastic frame is shown in Figure 1, and the rendering of the plastic basket is shown in Figure 2), so that there is a 12cm high space between the plastic frame and the bottom of the pool, which is conducive to draining the dirt in the sand. Spread 100-mesh mesh cloth on the plastic frame, and then spread 25cm thick fine sand on the mesh cloth. The diameter of the sand grains is ф600~800μm. Place 8 inflatable stones on the sand surface. A vertical drainage pipe is placed around the outer water outlet. The pipe height is 0.8m. When changing the water, the pool water flows out from the vertical drainage pipe.

In the embodiment of the present invention, the specific selection of Xishi tongue oysters is as follows: select Xishi tongue oysters with a shell length of more than 8.0cm (2 years old), complete shells, and strong shell closing as the oysters. After the oysters are rinsed, they are placed in a cement pool for ripening. Strengthen cultivation.

In the embodiment of the present invention, the water used in the breeding process of Xishi tongue broodstock is the shrimp pond water in the above-mentioned white shrimp breeding pond.

Example 1

During the breeding process of Xishi tongue broodstock, the stocking density is 16/m ² , add water (shrimp pond water) to the culture tank to the height of the drainage pipe 0.8m, change 1/2 of the water at 10:00 am every day, and use a water pump (2-inch water pump , flow rate is 15m ³ / hour) add shrimp pond water to the original water depth, pump the shrimp pond water directly with a pump at 6:00 pm and add it to the Xishi tongue broodstock culture pond, and allow the pond water to continue convection for 15 minutes to maintain a certain degree of transparency in the pond water. ; Clean the surface sand layer every 5 days, use a pipe to siphon away the dirty water, and then perform convection treatment (use a pump to extract shrimp pond water and add it to the broodstock culture pond, and allow the pond water to continue convection for 15 minutes to keep the pond water at a certain level. transparency). If dead clams are found during the breeding process, they should be picked out in time.

Example 2

During the breeding process of Xishi tongue broodstock, the stocking density is 18 individuals/m ² . Water is added to the culture tank (shrimp pond water) to the height of the drainage pipe 0.8m. 1/2 of the water is changed at 9:00 am every day, and a water pump (2-inch water pump) is used. , flow rate is 15m ³ / hour) add shrimp pond water to the original water depth; at 5:00 pm, directly pump the shrimp pond water and add it to the Xishi tongue broodstock culture pond, and let the pond water continue to convection for 15 minutes to maintain a certain degree of transparency in the pond water ; Clean the surface sand layer every 5 days, use a pipe to siphon away the dirty water, and then perform convection treatment (use a pump to extract shrimp pond water and add it to the broodstock culture pond, and allow the pond water to continue convection for 15 minutes to keep the pond water at a certain level. transparency). If dead clams are found during the breeding process, they should be picked out in time.

Example 3

During the breeding process of Xishi tongue broodstock, the stocking density is 15 individuals/m ² . Water is added to the culture tank (shrimp pond water) to the height of the drainage pipe 0.8m. 1/2 of the water is changed at 10:00 am every day, and a water pump (2-inch water pump) is used. , flow rate is 15m ³ / hour) add shrimp pond water to the original water depth; at 6:00 pm, directly pump the shrimp pond water and add it to the Xishi tongue broodstock culture pond, and let the pond water continue to convection for 15 minutes to maintain a certain degree of transparency in the pond water ; Clean the surface sand layer every 5 days, use a pipe to siphon away the dirty water, and then perform convection treatment (use a pump to extract shrimp pond water and add it to the broodstock culture pond, and allow the pond water to continue convection for 15 minutes to keep the pond water at a certain level. transparency). If dead clams are found during the breeding process, they should be picked out in time.

Example 4

During the breeding process of Xishi tongue broodstock, the stocking density is 17 individuals/m ² . Water is added to the culture tank (shrimp pond water) to the height of the drainage pipe 0.8m. 1/2 of the water is changed at 10:00 am every day, and a water pump (2-inch water pump) is used. , flow rate is 15m ³ / hour) add shrimp pond water to the original water depth; at 6:00 pm, pump the shrimp pond water and add it to the Xishi tongue broodstock culture pond, and let the pond water continue to convection for 15 minutes to maintain a certain degree of transparency in the pond water; Clean the surface sand layer every 5 days, use a pipe to siphon away the dirty water, and then perform convection treatment (use a pump to pump the shrimp pond water and add it to the broodstock culture pond, and let the pond water continue to convection for 15 minutes to keep the pond water at a certain level. transparency). If dead clams are found during the breeding process, they should be picked out in time.

Comparative example 1

The only difference from Example 1 is that the cultivation water is filtered seawater, the convection water is also filtered seawater every afternoon, and artificially cultivated monocystis algae (Zhanjiang et al.) are fed at 10:00 am and 6:00 pm every day Trichophyton, Chaetoceros mouleri, Platyphyllum subcordiformis, Chlorella).

Comparative example 2

The only difference from Example 1 is that the pool water is allowed to continue convection for 15 minutes every afternoon to adjust the water change to 1/2.

For Examples 1-4 and Comparative Examples 1-2, the time from the introduction of Xishi tongue brood oysters to their sexual maturity (4th age), the number of eggs laid by an individual for the first time, the survival rate of the first cultivation, the time from the first egg laying The time required to lay eggs for the second time, the number of eggs laid by individuals for the second time, and the survival rate of the second breeding were statistically calculated. The results are shown in Table 1.

Table 1

The present invention uses shrimp pond water as the culture water for Xishi tongue oysters. There is no need to feed additional feed during the cultivation process. The green algae, diatoms, golden algae and organic debris in the shrimp pond water can be used as feed for Xishi tongue oysters, which is nutritious. Comprehensive, it can meet the nutritional needs of Xishi tongue oysters and improve their disease resistance. At the same time, combined with reasonable water exchange and convection processes, the ripening speed of Xishi tongue oysters can be accelerated and their ovulation volume can be increased.

The above-described embodiments only describe the preferred modes of the present invention and do not limit the scope of the present invention. Without departing from the design spirit of the present invention, those of ordinary skill in the art can make various modifications to the technical solutions of the present invention. All deformations and improvements shall fall within the protection scope determined by the claims of the present invention.

Claims

A maturation-promoting method for cultivating Xishi tongue oysters using shrimp pond algae, which is characterized by including the following steps:

Put the Xishi tongue broodstock into a culture pond for maturation promotion, and add shrimp pond water to the culture pond to the height of the drainage pipe;

Daily management is as follows: change 1/2 of the water every morning, add shrimp pond water to the original water depth, directly extract shrimp pond water and add it to the culture pond in the afternoon, and allow the pond water to continue convection; regularly clean the sand layer on the surface.
A maturation-promoting method for cultivating Xishi tongue oysters using shrimp pond algae according to claim 1, characterized in that the stocking density of Xishi tongue oysters is 15-18 individuals/m 2 .
A maturation-promoting method for cultivating Xishi tongue oysters using shrimp pond algae according to claim 1, characterized in that the convection time is 12-18 minutes.
A maturation-promoting method for cultivating Xishi tongue oysters using shrimp pond algae according to claim 1, characterized in that the surface sand layer is cleaned every 5 days.
A maturation-promoting method for cultivating Xishi tongue oysters using shrimp pond algae according to claim 1, characterized in that the shrimp pond water is a breeding pond water for raising prawns.
A maturation-promoting method for cultivating Xishi tongue oysters using shrimp pond algae according to claim 1, characterized in that the shrimp pond water contains green algae, diatoms, golden algae and organic debris.