CN114600806B - Ripening promoting method for cultivating Xishi lingual parent shellfish by using pond algae - Google Patents

Ripening promoting method for cultivating Xishi lingual parent shellfish by using pond algae Download PDF

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CN114600806B
CN114600806B CN202210328927.2A CN202210328927A CN114600806B CN 114600806 B CN114600806 B CN 114600806B CN 202210328927 A CN202210328927 A CN 202210328927A CN 114600806 B CN114600806 B CN 114600806B
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pond
water
xishi
tongue
shellfish
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CN114600806A (en
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罗杰
王中铎
郭昱嵩
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Guangdong Ocean University
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Guangdong Ocean University
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Priority to PCT/CN2022/095873 priority patent/WO2023184693A1/en
Priority to LU502637A priority patent/LU502637B1/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a maturation promoting method for culturing Xishi tongue parent shellfish by using pond algae, and relates to the technical field of Xishi tongue culture. The method comprises the following steps: putting the parent Spanish tongue shellfish into a culture pond for ripening cultivation, and adding shrimp pond water into the culture pond to reach the height of a drain pipe; the daily management specifically comprises the following steps: changing 1/2 of water every morning, adding the shrimp pond water to the original water depth, extracting the shrimp pond water in the afternoon, adding the shrimp pond water into the culture pond, and continuously convecting the pond water for a certain time; the surface was cleaned periodically for a layer of sand. The method for culturing the Xishi tongue parent shellfish is simple and convenient to operate, the technology is easy to master, manpower and material resources can be saved, and the production cost is reduced.

Description

Ripening promoting method for cultivating Xishi lingual parent shellfish by using pond algae
Technical Field
The invention relates to the technical field of Xishi tongue culture, in particular to a maturation promoting method for culturing Xishi tongue parent shellfish by using pond algae.
Background
The western tongue is large-scale bivalve shellfish buried in shallow sea, and is used for eating unicellular algae and organic debris in water, commonly called as high-ranked imperial concubine snail. The Xishi tongue is large, high in meat content, tender and delicious in meat quality and rich in nutrition, and is a popular and rare edible shellfish. In recent years, the demand of the tongue of western province is increasing, and various predatory fishing (such as electric trawling, harrowing and other operation modes) lead to sharp reduction of yield and serious damage to resources. Therefore, the resource protection of the tongue of the western-style application is extremely important, the artificial seedling and breeding research of the tongue of the western-style application is an effective way for solving the current resource shortage, and the key of the artificial seedling is to obtain the mature parent shellfish of the tongue of the western-style application and hasten the parturition and discharge the sperm and egg. The traditional method for promoting the maturation of the tongue parent shell in Xishi mainly comprises the following steps: 1) Ripening is promoted by feeding artificially cultured unicellular algae; 2) Meanwhile, the water temperature is adjusted to promote the ripening. However, more manpower and material resources are needed for artificially culturing the unicellular algae, the growth of the algae is often greatly influenced by weather, the supply of the algae cannot be ensured, in addition, the artificially cultured unicellular algae is fed in a single variety, the nutrition is not comprehensive after the tongue is fed by the western medicine, and the maturation of the gonad of the tongue is not facilitated; and a large amount of energy is consumed for regulating and controlling the water temperature, and the production concept of low carbon and emission reduction is not met.
Disclosure of Invention
Based on the above discussion, the invention provides a maturation promoting method for culturing Xishi tongue parent shellfish by using shrimp pond algae, which realizes the maturation promoting effect of the Xishi tongue parent shellfish by using the shrimp pond algae and organic debris in a shrimp pond as bait of the Xishi tongue without regulating and controlling water temperature under natural conditions.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a ripening method for cultivating Xishi lingual parent shellfish by using pond algae, which comprises the following steps:
putting the Xishi tongue parent shellfish into a culture pond for ripening cultivation, and adding shrimp pond water into the culture pond to reach the height of a drain pipe;
the daily management specifically comprises the following steps: changing water 1/2 every morning, using water pump (2 inch water pump, flow rate is 15 m) 3 Per hour) pumping the shrimp pond water to the original water depth; directly extracting the shrimp pond water in the afternoon, adding the shrimp pond water into the culture pond, and continuously convecting the pond water; the surface was cleaned periodically for a layer of sand.
Further, the stocking density of the parent shell of the Xishi tongue is 15-18 per meter 2
Further, the convection time is 12-18 minutes.
Further, the surface sand layer was washed every 5 days.
Further, the shrimp pond water is water in an aquaculture pond for feeding prawns.
Further, the shrimp pond water contains green algae, diatoms and chrysophytes and organic debris.
The green algae, diatoms and chrysophytes and organic debris are all nutrients that facilitate ingestion by the tongue in western applications.
The prawn culture pond specifically comprises: laying a special film for cultivation at the bottom of the shrimp pond, and respectively placing waterwheel type automatic aerators on opposite corners of the pond; cleaning and sterilizing shrimp pond, adding seawater filtered by coarse sand, and applying inorganic nutritive salt for culturing algae, wherein the application amount is compound fertilizer (total nutrient is more than or equal to 45%, N-P) 2 O 5 -K 2 O=14-16-15)12-18g/m 3 Sodium silicate (Na) 2 SiO 3 )1-1.5g/m 3 Starting an aerator to fully mix the pond water with the nutritive salt; culturing for 7-8 days to make the algae in the shrimp pond grow up, and putting the Penaeus vannamei Boone fry when the pond water reaches a certain transparency. The shrimp pond management is carried out according to the following modes: adding nutrient salt for algae growth 10-15 days after shrimp larvae release, wherein the adding amount of the nutrient salt is compound fertilizer (total nutrient is more than or equal to 45%, N-P 2 O 5 -K 2 O=14-16-15)4-6g/m 3 0.3-0.5g/m of sodium silicate 3 (ii) a The residual bait left by feeding the shrimp feed and the excrement excreted along with the growth of the prawn individuals increase later, and the residual bait and the excrement of the prawns can be used as nutrient components of the algae; the aerator is started for 2 hours in the morning and afternoon respectively every day.
The invention discloses the following technical effects:
(1) The unicellular algae growing in the shrimp pond comprise green algae, diatom and chrysophyceae, are various in variety, and also comprise organic debris, which are high-quality biological baits for Xishi tongue, and meanwhile, the growth of the algae is slightly influenced by weather. Therefore, when the parent spat of the Xishi tongue is cultured, the shrimp pond algae and the organic debris are mainly fed, the nutrition is comprehensive, the Xishi tongue can be promoted to quickly mature, the sperm and egg can be discharged, and the sperm and egg discharge amount can be increased. And after the parent shellfish discharges the sperms and the eggs for the first time, the gonads of the parent shellfish can mature again to discharge the sperms and the eggs after a short period of intensive culture.
(2) The method for culturing the Xishi tongue parent shellfish is simple and convenient to operate, the technology is easy to master, manpower and material resources can be saved, and the production cost is reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required in the embodiments will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a photograph of a plastic frame used for cultivating the tongue-parent shell of Xishi according to the present invention;
FIG. 2 is a photograph of a culture pond for culturing Xishi tongue parent shellfish according to the present invention;
FIG. 3 is an effect diagram of the plastic frame paved at the bottom of the culture pond for culturing Xishi tongue parent shellfish according to the present invention.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but rather as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated herein by reference to disclose and describe the methods and/or materials in connection with which the documents are cited. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. It is intended that the specification and examples be considered as exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
The breeding site of the Xishu tongue is in the Zhen fighting Zhenzhouhong village in Zhanjiang province in Guangdong.
The shrimp pond in the embodiment of the invention is a prawn culture pond: the area of the shrimp pond is about 2000m 2 The depth of the pond is 1.5-1.7 m, a special film for cultivation is paved at the bottom of the pond, and a waterwheel type aerator with the power of 0.75kv is respectively arranged at the opposite corners of the pond. Shrimp pond intakeThoroughly cleaning and sterilizing, adding seawater filtered by coarse sand to a depth of 1.4m, and adding inorganic nutritive salt for culturing algae at a dosage of compound fertilizer 15g/m 3 Sodium silicate (Na) 2 SiO 3 )1.3g/m 3 And starting the aerator to fully mix the pond water with the nutritive salt. The algae in the pond can grow up after being cultured for 7 to 8 days, and when the pond water reaches certain transparency, the south America white prawn seedlings with the body length of about 1cm are thrown, and the number of the larvae is 9 ten thousand.
And (3) shrimp pond management: adding nutritive salt for algae growth 12 days after the shrimp larvae are released, wherein the adding amount of the nutritive salt is 1/3 of the initial adding amount. The residual bait left by feeding the shrimp feed and the excrement excreted along with the growth of the individual prawns are increased, so that the residual bait and the excrement of the prawns can be used as nutrient components of the algae. The aerator is started for 2 hours at 7 am and 18 pm each day.
The culture pond for culturing the Xishi tongue parent shellfish in the embodiment of the invention specifically comprises the following steps: indoor cement pool (as figure 2), pool bottom area 20m 2 The depth of the pool is 1.0m, and a plastic frame (the plastic frame is shown in figure 1, and the effect picture of laying the plastic basket is shown in figure 2) with the specification of 65cm long, 45cm wide and 12cm high is additionally arranged at the bottom of the pool, so that a space with the height of 12cm is formed between the plastic frame and the bottom of the pool, and the dirt in sand can be discharged conveniently. 100 meshes of mesh cloth is paved on the plastic frame, fine sand with the thickness of 25cm is paved on the mesh cloth, the diameter of the sand is 600-800 mu m, and 8 inflatable stones are placed on the sand surface. The vertical drain pipe is sleeved at the water outlet outside, the pipe height is 0.8m, and pool water flows out from the vertical drain pipe when water is changed.
The selection of the Xishi tongue parent shellfish in the embodiment of the invention specifically comprises the following steps: selecting Xishi tongue with shell length of more than 8.0cm (age of 2), complete shell and strong shell closure as parent shell, washing parent shell, and placing parent shell in cement pool to make accelerated growth.
In the embodiment of the invention, all the water used in the culture process of the Xishi tongue parent shellfish is the shrimp pond water in the penaeus vannamei culture pond.
Example 1
In the process of culturing the parent shellfish of the Xishi tongue, the stocking density is 16/m 2 And adding water (shrimp pond water) into the culture pond to the height of a water discharge pipe of 0.8m, changing water 1/2 at 10 am every day, and using a water pump (2-inch water pump)At a flow rate of 15m 3 Hour) adding the shrimp pond water to the original water depth, directly pumping the shrimp pond water by using a water pump to be added into a Xishi tongue parent shellfish culture pond at 6 pm, and allowing the pond water to continuously convect for 15 minutes to keep certain transparency of the pond water; the surface sand layer was washed every 5 days, and the dirty water was siphoned off by a tube, followed by convection treatment (shrimp pond water was pumped by a water pump to the parent shellfish culture pond and the pond water was allowed to flow for 15 minutes, keeping the pond water transparent to some extent). Dead shellfish can be found in the feeding process and picked out in time.
Example 2
During the cultivation process of the Xishi tongue parent shellfish, the stocking density is 18/m 2 Adding water (shrimp pond water) into the culture pond until the height of a water discharge pipe is 0.8m, changing water for 1/2 at 9 am every day, and using a water pump (2-inch water pump, the flow is 15 m) 3 Hour) adding the shrimp pond water to the original water depth; pumping the shrimp pond water directly by using a water pump to be added into a Xishi tongue parent shellfish culture pond at the afternoon of 5 hours, and continuously convecting the pond water for 15 minutes to keep certain transparency of the pond water; the surface sand layer was washed every 5 days, and the dirty water was siphoned off by a tube, followed by convection treatment (shrimp pond water was pumped by a water pump to the parent shellfish culture pond and the pond water was allowed to flow for 15 minutes, keeping the pond water transparent to some extent). Dead shellfish can be found in the feeding process and picked out in time.
Example 3
During the cultivation process of the Xishi tongue parent shellfish, the stocking density is 15/m 2 And adding water (shrimp pond water) into the culture pond until the height of a water discharge pipe is 0.8m, changing water 1/2 at 10 am every day, and using a water pump (2 inch water pump, the flow rate is 15 m) 3 Hour) adding the shrimp pond water to the original water depth; pumping the shrimp pond water directly by using a water pump to be added into a Xishi tongue parent shellfish culture pond at 6 pm, and continuously convecting the pond water for 15 minutes to keep certain transparency of the pond water; the surface sand layer was washed every 5 days, and the dirty water was siphoned off by a tube, followed by convection treatment (shrimp pond water was pumped by a water pump to the parent shellfish culture pond and the pond water was allowed to flow for 15 minutes, keeping the pond water transparent to some extent). Dead shellfish can be found in the feeding process and picked out in time.
Example 4
During the cultivation process of the parent shell of the Xishi tongue, the parent shell is bred denselyDegree is 17/m 2 And adding water (shrimp pond water) into the culture pond until the height of a water discharge pipe is 0.8m, changing water 1/2 at 10 am every day, and using a water pump (2 inch water pump, the flow rate is 15 m) 3 Hour) adding the shrimp pond water to the original water depth; pumping the shrimp pond water into a Xishi tongue parent shellfish culture pond by using a water pump at 6:00 pm, and continuously convecting the pond water for 15 minutes to keep the pond water to have certain transparency; the surface sand layer was washed every 5 days, and the dirty water was siphoned off by a tube, followed by convection treatment (shrimp pond water was pumped by a water pump to the parent shellfish culture pond and the pond water was allowed to flow for 15 minutes, keeping the pond water transparent to some extent). Dead shellfish can be found in the feeding process and picked out in time.
Comparative example 1
The difference from example 1 is only that the culture water is filtered seawater, the convection water is also filtered seawater every afternoon, and artificially cultured unicellular algae (flagellates such as flagellates, chaetoceros muelleri, oblate tenella, chlorella) are fed at 10 am and 6 am.
Comparative example 2
The difference from example 1 was only that the continuous convection of the pool water for 15 minutes each afternoon was adjusted to 1/2 of the water change.
The time taken from introduction of the lingual parent mussels of Xishi in examples 1-4 and comparative examples 1-2 to their sexual maturity (age 4), the first individual egg laying amount, the first breeding survival rate, the time required from the first egg laying to the second egg laying, the second individual egg laying amount and the second breeding survival rate were counted, and the results are shown in Table 1.
TABLE 1
Figure BDA0003574512380000071
Figure BDA0003574512380000081
The culture water of the Xishi tongue parent shellfish is shrimp pond water, no bait is required to be fed in the culture process, green algae, diatom, golden algae and organic debris in the shrimp pond water can be used as the bait of the Xishi tongue parent shellfish, the nutrition is comprehensive, the nutritional requirements of the Xishi tongue parent shellfish can be met, and the disease resistance of the Xishi tongue parent shellfish can be improved. Meanwhile, reasonable water changing and convection processes are matched, so that the maturing speed of the parent shell of the Xishi tongue can be increased, and the egg discharging amount of the parent shell of the Xishi tongue can be increased.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims (5)

1. A ripening method for cultivating Xishi lingual parent shellfish by utilizing pond algae is characterized by comprising the following steps:
putting the Xishi tongue parent shellfish into a culture pond for ripening cultivation, and adding shrimp pond water into the culture pond to reach the height of a drain pipe;
the daily management specifically comprises the following steps: changing 1/2 of the water in the morning every day, adding the shrimp pond water to the original water depth, directly extracting the shrimp pond water in the afternoon and adding the shrimp pond water into the culture pond, and continuously convecting the pond water; cleaning the sand layer on the surface periodically;
the convection time is 12-18 minutes.
2. The ripening method of using pond algae for cultivating the lingual shellfish of Xishi as claimed in claim 1, wherein the stocking density of the lingual shellfish of Xishi is 15-18 per m 2
3. The method for promoting maturation of Limnophilus sempervirens using Aphanothece crambe according to claim 1, wherein the sand layer on the surface is washed every 5 days.
4. The method for promoting maturation of the Xishi lingual shellfish using pond algae as claimed in claim 1, wherein the pond water is pond water for raising prawns.
5. The method as claimed in claim 1, wherein the pond water contains chlorella, diatom, and chrysophyceae and organic debris.
CN202210328927.2A 2022-03-31 2022-03-31 Ripening promoting method for cultivating Xishi lingual parent shellfish by using pond algae Active CN114600806B (en)

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CN202210328927.2A CN114600806B (en) 2022-03-31 2022-03-31 Ripening promoting method for cultivating Xishi lingual parent shellfish by using pond algae
PCT/CN2022/095873 WO2023184693A1 (en) 2022-03-31 2022-05-30 Maturation promotion method for cultivating mactra antiquata parent shellfishes by using shrimp pond algae
LU502637A LU502637B1 (en) 2022-03-31 2022-05-30 Method for promoting maturation of coelomactra antiquata parent shellfish cultured by shrimp pond algae

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