EA201800291A1 - BIOCHIP FOR MULTIPLEX ANALYSIS AND METHOD OF STUDYING CELLS FOR DIAGNOSTICS OF ONCOLOGICAL DISEASES - Google Patents
BIOCHIP FOR MULTIPLEX ANALYSIS AND METHOD OF STUDYING CELLS FOR DIAGNOSTICS OF ONCOLOGICAL DISEASESInfo
- Publication number
- EA201800291A1 EA201800291A1 EA201800291A EA201800291A EA201800291A1 EA 201800291 A1 EA201800291 A1 EA 201800291A1 EA 201800291 A EA201800291 A EA 201800291A EA 201800291 A EA201800291 A EA 201800291A EA 201800291 A1 EA201800291 A1 EA 201800291A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- biochip
- cells
- minutes
- nutrient medium
- multiplex analysis
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
Предлагаемая группа изобретений относится к области медицины, а именно к диагностике, и может быть использована для диагностики онкологических заболеваний. Биочип для мультиплексного анализа, содержит прозрачную подложку из стекла, на которую нанесено покрытие, создающее положительный заряд на поверхности стекла, в тестовые ячейки внесены антитела, конъюгированные с флуоресцентными метками Cy-3, и реагент, препятствующий высыханию. Рабочая поверхность биочипа покрыта воздухонепроницаемой стерильной пленкой. Предварительная подготовка клеток из серозной жидкости, клеток из геморрагической жидкости или клеток мочи с осадком включает центрифугирование, удаление надосадочной жидкости и ресуспензирование осадка с питательной средой, при исследовании взвеси клеток, полученных из материала тонкоигольной аспирационной биопсии и клеток, полученных из соскоба с резецированной опухоли до ее помещения в формалин, пунктат помещают в пробирку Эппендорфа объемом 0,8 мл с питательной средой, проводят центрифугирование в течение 5 мин при скорости 1500 об/мин и внесение осадка в тестовые ячейки биочипа, после этого биочип с внесенными клетками различного биоматериала помещают в шейкер на 2-3 мин, затем в термостат на 25 мин при температуре 37°C. После чего ополаскивают биочип в буферном растворе, высушивают на воздухе и проводят флуоресцентный анализ. Предлагаемая группа изобретений позволяет расширить возможности диагностики за счет возможности анализа большого перечня клеточного материала.The proposed group of inventions relates to the field of medicine, namely to diagnosis, and can be used to diagnose cancer. Biochip for multiplex analysis, contains a transparent substrate of glass, which is coated with a positive charge on the glass surface, antibodies conjugated with Cy-3 fluorescent labels and a reagent that prevents drying are introduced into the test cells. The working surface of the biochip is covered with an airtight sterile film. Preliminary preparation of cells from serous fluid, cells from hemorrhagic fluid, or urine cells with sediment includes centrifuging, removing the supernatant and re-suspending the pellet with the nutrient medium, when examining the suspension of cells obtained from fine-needle aspiration biopsy material and cells obtained from scraping resected tumors to putting it in formalin, punctate is placed in a 0.8 ml Eppendorf tube with a nutrient medium, centrifuged for 5 minutes at a speed of 1500 rev / yn and making the precipitate into test cell biochip, the biochip thereafter amended various cells biomaterial placed on a shaker for 2-3 minutes, then in a thermostat for 25 min at 37 ° C. Then rinse the biochip in a buffer solution, air dry and conduct a fluorescent analysis. The proposed group of inventions allows you to extend the capabilities of the diagnosis due to the possibility of analyzing a large list of cellular material.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/RU2016/000317 WO2017204674A1 (en) | 2016-05-26 | 2016-05-26 | A biochip for multiplex analysis and a method of studying cells in the diagnosis of oncological diseases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EA201800291A1 true EA201800291A1 (en) | 2018-09-28 |
Family
ID=60412797
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201800291A EA201800291A1 (en) | 2016-05-26 | 2016-05-26 | BIOCHIP FOR MULTIPLEX ANALYSIS AND METHOD OF STUDYING CELLS FOR DIAGNOSTICS OF ONCOLOGICAL DISEASES |
Country Status (2)
| Country | Link |
|---|---|
| EA (1) | EA201800291A1 (en) |
| WO (1) | WO2017204674A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2712080C1 (en) * | 2019-05-14 | 2020-01-24 | Федеральное государственное автономное образовательное учреждение высшего образования "Дальневосточный федеральный университет" (ДВФУ) | Method for cytologic examination in differential diagnosis of nodular thyroid formations |
| CN114323878A (en) * | 2021-12-29 | 2022-04-12 | 上海益诺思生物技术股份有限公司 | Formalin fixation method for liver micronucleus test |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6656697B1 (en) * | 1998-09-28 | 2003-12-02 | Lifescan, Inc. | Diagnostics based on tetrazolium compounds |
| RU2292551C1 (en) * | 2005-09-26 | 2007-01-27 | Государственное учреждение Дальневосточный научный центр физиологии и патологии дыхания Сибирского отделения Российской академии медицинских наук | Early stage diagnosis method for determining prenatal infection in newborns |
| RU137188U1 (en) * | 2013-01-31 | 2014-02-10 | Государственное бюджетное образовательное учреждение высшего профессионального образования "Нижегородская государственная медицинская академия" Министерства здравоохранения Российской Федерации (ГБОУ ВПО НижГМА МЗ России) | BIOCHIP FOR DIAGNOSTICS IN THE FIELD OF MEDICINE |
| RU142470U1 (en) * | 2014-02-07 | 2014-06-27 | Святослав Владимирович Зиновьев | BIOCHIP FOR MULTIPLEX ANALYSIS |
-
2016
- 2016-05-26 EA EA201800291A patent/EA201800291A1/en unknown
- 2016-05-26 WO PCT/RU2016/000317 patent/WO2017204674A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017204674A1 (en) | 2017-11-30 |
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