CN214385784U

CN214385784U - Cell freezing tube

Info

Publication number: CN214385784U
Application number: CN202120437545.4U
Authority: CN
Inventors: 吴道贫; 莫乾强; 谢秀凤; 邹洁宜; 练劲
Original assignee: Guangzhou Zhunyou Biological Technology Co ltd
Current assignee: Guangzhou Zhunyou Biological Technology Co ltd
Priority date: 2021-03-01
Filing date: 2021-03-01
Publication date: 2021-10-15
Anticipated expiration: 2031-03-01

Abstract

The utility model relates to a cell cryopreserving pipe, including body, pipe cap, locating lever and support flat board, the one end of body has the mouth of pipe, the pipe cap can dismantle connect in thereby the one end of body is sealed the mouth of pipe, the locating lever connect in the pipe cap is kept away from the one end of body, support flat board a side connect in the locating lever is kept away from the one end of pipe cap. The utility model discloses a cell cryopreserving pipe is when using, can clip the locating lever through pressing from both sides the device and get and put the operation, and the support flat board of being connected with the locating lever then can prevent that the cell cryopreserving pipe from getting the time landing of getting, opens the cell cryopreserving pipe in addition and when operating, and the pipe cap can invert on the desktop through supporting the flat board, prevents that the pipe cap from rolling at will, has avoided the pipe cap to lose or be contaminated, has further improved cell cryopreserving or cell resuscitation's efficiency and quality.

Description

Cell freezing tube

Technical Field

The utility model relates to the technical field of medical equipment, especially, relate to a cell cryopreserving pipe.

Background

Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the cells to survive, grow and reproduce and maintain main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched. If the tissue block is cultured, the tissue block is directly inoculated to the bottom of a culture vessel, the tissue block can be firmly attached to the bottom after several hours, and then the culture medium is added. For cell culture, cell counting is generally performed before the culture vessel is inoculated, and the culture vessel is inoculated with a certain amount (expressed by cells per milliliter) and directly added with culture medium according to requirements. After the cells enter the culture vessel, the cells are immediately placed into an incubator, so that the cells enter a growth state as early as possible. The cells being cultured should be observed at regular intervals, including whether the cells are growing well, whether the morphology is normal, whether there is contamination, whether the pH of the medium is too acidic or too basic (indicated by phenol red indicator), and also for the temperature of the culture and the CO₂The concentration was also checked periodically. In general, after the primary culture enters the culture, a period of incubation period (from hours to tens of days) exists, cells generally do not divide in the incubation period, but can adhere to the wall and swim away, and after the incubation period, the cells enter a vigorous division growth period. After the cells grow to the bottom of the bottle, subculturing is carried out, the cells in one bottle are digested and suspended, and then are divided into two to three bottles for continuous culture, and each subculturing is called as 'one generation'. Diploid cells generally can only pass through dozens of generations, and then turnThe chemolines or cell lines can be passaged indefinitely. Transformed cells may have malignant properties or may be immortal only and not malignant. Culturing growing cells is a good material for performing various biomedical experiments.

In order to preserve cells, particularly mutant cells or cell lines, which are not readily available, the cells are cryopreserved. Cell cryopreservation is a method frequently used in cell culture processes, and long-term preservation of cells is basically accomplished by filling a cryopreservation tube into liquid nitrogen. Because the boiling point of liquid nitrogen is low, and the stability and the safety are good, the liquid nitrogen can inhibit the biochemical activity in cells and reduce the metabolism at the temperature. The temperature of cryopreservation is generally the temperature of liquid nitrogen, namely-196 ℃, cells are collected into a cryopreservation tube, a culture medium containing a protective agent (generally dimethyl sulfoxide or glycerol) is added into the tube, and the tube is frozen at a certain cooling speed and finally stored in the liquid nitrogen. At very low temperatures, the time for which the cells are stored is almost unlimited. The recovery generally adopts a fast melting method, namely, after taking out the freezing tube from liquid nitrogen, immediately putting the freezing tube into water with the temperature of 37 ℃ to rapidly melt the freezing tube within one minute, and then transferring the cells into a culture vessel for culture. The selection of protective agents, the cell density, the cooling speed, the temperature during recovery, the melting speed and the like in the freezing storage process influence the cell activity. The freezing storage tube is a common freezing storage container used in cell transfer, so that the freezing storage tube has higher requirements. In biological experiments, the cryopreservation tube is not used independently often, but is used together with a matched cryopreservation box, and the cryopreservation box mainly plays a role in fixing and primarily classifying.

However, the conventional cryopreservation tube has the disadvantage of long time for taking and placing in the cryopreservation and taking and placing processes, and the long-time operation can reduce the biological activity of cells in the cryopreservation tube, and simultaneously influences the temperature of other cryopreservation tubes in the whole cryopreservation box, thereby indirectly damaging the cryopreservation effect of a large number of cell samples.

SUMMERY OF THE UTILITY MODEL

Therefore, there is a need for a cell cryopreservation tube with more convenient operation.

The utility model provides a cell cryopreserving pipe, includes body, pipe cap, locating lever and supports the flat board, the one end of body has the mouth of pipe, thereby the pipe cap can dismantle connect in thereby the one end of body seals the mouth of pipe, the locating lever connect in the pipe cap is kept away from the one end of body, support dull and stereotyped a side connect in the locating lever is kept away from the one end of pipe cap.

Because the cell cryopreserved pipe is arranged together very closely usually in cryopreserved box, get the cell cryopreserved pipe when adjacent cell cryopreserved pipe space is too little, be difficult to get fast accurately when taking gloves and put, and frost can appear on the surface of cell cryopreserved pipe, even press from both sides with tweezers etc. and get the device and also slide easily, be difficult to steadily get fast and put, this just leads to getting out or putting back the cell cryopreserved pipe from cryopreserved box the degree of difficulty great, the spent time is longer. The utility model discloses a cell cryopreserving pipe is when using, can clip the locating lever through pressing from both sides the device and get and put the operation, and the support flat board of being connected with the locating lever then can prevent that the cell cryopreserving pipe from getting the time landing of getting, opens the cell cryopreserving pipe in addition and when operating, and the pipe cap can invert on the desktop through supporting the flat board, prevents that the pipe cap from rolling at will, has avoided the pipe cap to lose or be contaminated, has further improved cell cryopreserving or cell resuscitation's efficiency and quality. Therefore, the utility model discloses a cell cryopreserving pipe can be convenient take out or put back from cryopreserving box, has improved work efficiency in the use, has reduced the operation degree of difficulty, has guaranteed the biological activity of cell.

In one embodiment, a side of the support plate away from the positioning rod is provided with a plurality of first support legs.

In one embodiment, the first support legs are uniformly spaced along the periphery of the support plate.

In one embodiment, one end of the first support legs, which is far away from the support flat plate, extends towards the outer side of the support flat plate in an inclined mode.

In one embodiment, the number of the first legs is at least three.

In one embodiment, the supporting flat plate is circular, and the positioning rod is connected with the center of the supporting flat plate and is perpendicular to the supporting flat plate.

In one embodiment, the pipe body is cylindrical, an external thread is arranged at one end, away from the positioning rod, of the pipe cap, and an internal thread matched with the external thread is arranged at one end, close to the pipe orifice, of the pipe body.

In one embodiment, the device further comprises a sealing rubber ring, and the sealing rubber ring is sleeved at one end, far away from the positioning rod, of the tube cap.

In one embodiment, a plurality of second support legs are arranged at one end of the tube body away from the nozzle.

In one embodiment, the outer side wall of the pipe cap is also provided with concave-convex textures.

Drawings

FIG. 1 is a schematic structural diagram of a cell cryopreservation tube according to an embodiment;

FIG. 2 is an exploded view of the cell cryopreservation tube shown in FIG. 1.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise. In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art. In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature. It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

As shown in fig. 1-2, the cell cryopreservation tube 100 of an embodiment of the present invention includes a tube body 10, a tube cap 20, a positioning rod 30 and a supporting plate 40, wherein one end of the tube body 10 has a tube opening, the tube cap 20 can be detachably connected to one end of the tube body 10 to seal the tube opening, the positioning rod 30 is connected to one end of the tube cap 20 far away from the tube body 10, and a side surface of the supporting plate 40 is connected to one end of the positioning rod 30 far away from the tube cap 20.

Because the cell cryopreserved pipe is arranged together very closely usually in cryopreserved box, get the cell cryopreserved pipe when adjacent cell cryopreserved pipe space is too little, be difficult to get fast accurately when taking gloves and put, and frost can appear on the surface of cell cryopreserved pipe, even press from both sides with tweezers etc. and get the device and also slide easily, be difficult to steadily get fast and put, this just leads to getting out or putting back the cell cryopreserved pipe from cryopreserved box the degree of difficulty great, the spent time is longer. The utility model discloses a cell cryopreserving pipe 100 is when using, can clip locating lever 30 through pressing from both sides the device and get and put the operation, the support flat plate 40 of being connected with locating lever 30 then can prevent that cell cryopreserving pipe 100 from pressing from both sides the landing when getting, opening cell cryopreserving pipe 100 and when operating in addition, cap 20 can invert on the desktop through support flat plate 40, prevent cap 20 from rolling at will, avoided cap 20 to lose or be contaminated, further improved cell cryopreserving or cell resuscitative efficiency and quality. Therefore, the utility model discloses a cell cryopreserving pipe 100 can be convenient take out or put back from cryopreserving box, has improved work efficiency in the use, has reduced the operation degree of difficulty, has guaranteed the biological activity of cell.

In one specific example, a side of the support plate 40 away from the positioning rod 30 is provided with a plurality of first legs 41. In this way, when the cell cryopreservation tube 100 is opened for operation, the cap 20 can be inverted on a table top through the support plate 40, and the placement of the cap 20 can be more stable by using the plurality of first support legs 41, and meanwhile, the contact surface between the cap 20 and the table top is reduced, and the risk of being polluted by the table top is reduced.

In a specific example, the plurality of first legs 41 are uniformly spaced along the circumference of the support plate 40, so that the stability of the cap 20 in the inverted state can be improved.

In a specific example, one end of the plurality of first legs 41 away from the support plate 40 extends obliquely to the outer side of the support plate 40. In this way, each first leg 41 extends obliquely to the outer side of the support plate 40, and is less prone to toppling when the cap 20 is placed upside down, so that stability is further increased.

In one particular example, the number of first legs 41 is at least three. It is understood that the number of the first legs 41 is not limited thereto, and may be adjusted as needed.

In one specific example, the support plate 40 is circular, and the positioning rod 30 is connected to a center of the support plate 40 and perpendicular to the support plate 40. Thus, the tube cap 20 is less prone to toppling when being placed upside down, and stability of the tube cap 20 when being placed upside down is improved.

In a specific example, the tube body 10 is cylindrical, an external thread is arranged at one end of the tube cap 20 far away from the positioning rod 30, an internal thread matched with the external thread is arranged at one end of the tube body 10 close to the tube opening, and the tube body 10 and the tube cap 20 can be detachably connected through thread matching. It is understood that the shape of the tube body 10 is not limited thereto, and the detachable connection manner of the cap 20 and the tube body 10 is not limited thereto and may be adjusted as needed.

Optionally, the cell freezing tube 100 further includes a sealing rubber ring 50, and the sealing rubber ring 50 is sleeved on one end of the tube cap 20 away from the positioning rod 30. Thus, when the cap 20 is connected to the tube 10, the sealing performance of the cell cryopreservation tube 100 can be effectively improved, and the entry of contaminants and the outflow of cell samples can be prevented.

Optionally, the one end that the body 10 kept away from the mouth of pipe is equipped with a plurality of second stabilizer blades 11, so body 10 can stand upright on the desktop, helps reducing the operation degree of difficulty, strengthens the practicality greatly. Optionally, the bottom wall of the tube body 10 is arc-shaped, and the second leg 11 is formed by extending the sidewall of the tube body 10 in a direction away from the nozzle until protruding out of the bottom wall of the tube body 10. Optionally, the number of second legs 11 is at least three. It will be appreciated that the number of second legs 11 is not limited thereto and may be adjusted as desired.

In one embodiment, the outer sidewall of the cap 20 is also textured to increase friction for rotational operation. Specifically, the asperities are formed by a plurality of longitudinally extending stripe-shaped projections arranged in parallel at intervals. In one embodiment, the side walls of the positioning rod 30 are textured to increase friction for gripping.

Alternatively, the positioning rod 30 is cylindrical and has a smaller diameter than the cap 20 and the body 10. Optionally, the height of the positioning rod 30 is 0.5cm to 1.5cm, and the diameter is 0.2cm to 0.5 cm. Optionally, the positioning rod 30 and the supporting plate 40 are made of polypropylene or high-density polyethylene, and have the advantages of no odor, no toxicity, excellent low-temperature resistance, good chemical stability, resistance to corrosion of most acids and alkalis, insolubility in common solvents at normal temperature, small water absorption and excellent electrical insulation.

In one embodiment, the outer wall of the tube 10 is provided with graduations and the tube 10 is transparent so that the volume of liquid in the tube can be easily observed.

In one embodiment, the outer walls of the tube body 10 and the tube cap 20 are provided with an insulating layer, so that the inner and outer environments of the cell cryopreservation tube 100 can be relatively isolated, and the influence of the outer environment on the cell sample in the tube can be reduced.

The utility model discloses a cell cryopreserving pipe 100 is when using, can clip locating lever 30 through pressing from both sides the device and get and put the operation, the support flat plate 40 of being connected with locating lever 30 then can prevent that cell cryopreserving pipe 100 from pressing from both sides the landing when getting, opening cell cryopreserving pipe 100 and when operating in addition, cap 20 can invert on the desktop through support flat plate 40, prevent cap 20 from rolling at will, avoided cap 20 to lose or be contaminated, further improved cell cryopreserving or cell resuscitative efficiency and quality. In addition, the cell freezing tube 100 can be erected on a table, which helps to reduce the difficulty of work and greatly enhance the practicability. Therefore, the utility model discloses a cell cryopreserving pipe 100 can be convenient take out or put back from cryopreserving box, improved work efficiency in the use, reduced the operation degree of difficulty, improved the cell cryopreserving and the quality of cell recovery, guaranteed the biological activity of cell.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. The utility model provides a cell cryopreserving pipe, its characterized in that, includes body, pipe cap, locating lever and supports the flat board, the one end of body has the mouth of pipe, thereby the pipe cap can dismantle connect in thereby the one end of body seals the mouth of pipe, the locating lever connect in the pipe cap is kept away from the one end of body, support dull and stereotyped a side connect in the locating lever is kept away from the one end of pipe cap.

2. The cell cryopreservation tube of claim 1, wherein a side of the support plate away from the positioning rod is provided with a plurality of first support legs.

3. The cell cryopreservation tube of claim 2, wherein the first legs are uniformly spaced along the periphery of the support plate.

4. The cell cryopreservation tube of claim 3, wherein one end of the first legs, which is far away from the support plate, extends obliquely to the outer side of the support plate.

5. The cell cryopreservation tube of claim 3, wherein the number of the first legs is at least three.

6. The cell cryopreservation tube of any one of claims 1 to 5, wherein the support plate is circular, and the positioning rod is connected with the center of the support plate and is perpendicular to the support plate.

7. The cell freezing tube according to any one of claims 1 to 5, wherein the tube body is cylindrical, an external thread is provided at one end of the tube cap away from the positioning rod, and an internal thread for matching with the external thread is provided at one end of the tube body close to the tube opening.

8. The cell cryopreservation tube of any one of claims 1 to 5, further comprising a sealing rubber ring, wherein the sealing rubber ring is sleeved at one end of the tube cap far away from the positioning rod.

9. The cell freezing tube according to any one of claims 1 to 5, wherein a plurality of second legs are provided at an end of the tube body away from the tube opening.

10. The cell cryopreservation tube of any one of claims 1 to 5, wherein the outer side wall of the tube cap is further provided with a concave-convex texture.