CN208159973U - A kind of novel closed-type glass freezing load bar - Google Patents

A kind of novel closed-type glass freezing load bar Download PDF

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Publication number
CN208159973U
CN208159973U CN201820296817.1U CN201820296817U CN208159973U CN 208159973 U CN208159973 U CN 208159973U CN 201820296817 U CN201820296817 U CN 201820296817U CN 208159973 U CN208159973 U CN 208159973U
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China
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embryo
pipe
places
metal column
type glass
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CN201820296817.1U
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Chinese (zh)
Inventor
郝桂敏
赵志明
曹金凤
杜元杰
周亮
朱序理
王跃
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Second Hospital of Hebei Medical University
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Second Hospital of Hebei Medical University
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Abstract

The utility model relates to a kind of novel embryo vitrifying freezes to carry bar, belong to field of biomedicine.It includes outer tube, embryo placement pipe, isolated tube, metal column that the novel closed-type glass freezing, which carries bar,;The metal column is placed in the outer tube bottom;The isolated tube is placed in metal column upper layer;The embryo places pipe and is placed in isolated tube upper layer, and embryo places tube top end away from outer tube nozzle 1.3cm, and nozzle is sealed.Novel closed-type glass freezing described in the utility model carries bar, can avoid in liquid nitrogen that there may be the cross contaminations between the pollution of embryo caused by microbial contamination or embryo, and the utility model manufacture craft is simple, low in cost.It in Embryo freezing preservation, may make embryo's vertical immersion in liquid nitrogen, as liquid nitrogen fluid change can occur to sink accordingly or rise, embryo will not be caused to damage since liquid nitrogen is reduced.

Description

A kind of novel closed-type glass freezing load bar
Technical field
The utility model relates to a kind of devices more particularly to a kind of novel embryo vitrifying freeze to carry bar, belong to biological doctor Field.
Background technique
Embryo cryopreservation is that Issues of Human Assisted Reproductive Technologies is one of the important technical that patient saves embryo, it can effectively be mentioned High each accumulation pregnancy rate for taking the ovum period.Vitrification can effectively reduce cell freezing damage, improve embryo or Survival rate and Pregnancy Success rate after egg freezing recovery.It is a kind of current assisted reproductive technology field commonly fast freezing embryo The method of tire or ovum.But in the laboratory of different Reproductive Medicine Centers, the Freezing-thawing effect of embryo is not quite similar, It is related bar may to be carried to the freezing that different laboratories uses.Existing most domestic reproductive center mainly carries bar, half using open Three kinds of glass freezings of closed and closed commercialization carry bar.It is semiclosed be first intrusion liquid nitrogen it is re-closed, open loads bar with Semi-enclosed load bar contact embryo directly with liquid nitrogen, it is thus possible to so that embryo is there is safety hidden Suffer from.For human reproduction center, the safety of embryo is extremely important, it is understood that there may be the cross contamination between embryo, or by In liquid nitrogen, there are embryos caused by microbial contamination to pollute, and therefore, closed freezing carrier uses particularly necessary.
However existing closed carrier structure multiplicity, complicated for operation, price is significantly higher than semi-enclosed load bar and opening Carry bar.Therefore, easy to operate, cheap vitrified frozen vector is the product that embryo experiments room is badly in need of.
Utility model content
Technical problem to be solved in the utility model is that the defect of the prior art is overcome to provide a kind of novel closed-type glass Glassization freezing carries bar.The novel closed-type glass freezing carries survival of the bar after guaranteeing embryo's safety, embryo cryopreservation recovery While rate and Pregnancy Success rate, cost can be reduced, simplifies operation.Feeding device, the feeding device have the at low cost, service life High, manufacture is easy, is conducive to the advantages that various materials alternating is filling.
Technical problem described in the utility model is realized by the following technical solutions.
A kind of novel closed-type glass freezing described in the utility model carries bar, including outer tube, embryo place pipe, isolation Pipe, metal column;The metal column is placed in the outer tube bottom;The isolated tube is placed in metal column upper layer;The embryo is put It sets pipe and is placed in isolated tube upper layer, embryo places tube top end away from outer tube nozzle 1.3cm, and nozzle is sealed.
Preferably, a kind of above-mentioned novel closed-type glass freezing carries bar, and the outer tube is open type glassization freezing Bar is carried, the length is 13.2cm, diameter 0.3cm;The metal column is stainless steel metal column, and the length is 1.5cm, diameter is 0.2cm;The embryo place pipe diameter be 0.2cm, length 6.6cm, it is that embryo places end, the embryo that embryo, which places pipe one end, Placing end is that embryo places groove;The embryo places groove and places the angle that pipe one end one length of beveling is 2cm by embryo It is formed;The isolated tube is intermediate closed pipe, and the length is 2cm, intermediate confining bed length is 1.4cm.
Further, above-mentioned novel closed-type glass freezing carries bar, and the embryo places groove for placing embryo.Embryo Tire places pipe and is greater than 180 ° in the angle that embryo places the nozzle cross section at end, that is to say, that the part chamfer is put in embryo The angle shown on the nozzle cross section at end is set less than 180 °, if embryo can occur after angle is excessive places end insertion isolated tube It is interior, cause embryo damage.The metal column carries bar lower end gravity for increasing novel closed-type glass freezing, can make novel sealing Enclosed glass freezing carries bar and is immersed in liquid nitrogen perpendicular to embryo's long-time in liquid nitrogen, is conducive to, and is conducive to embryo for a long time It saves.
Novel closed-type glass freezing described in the utility model carries bar, can avoid in liquid nitrogen that there may be microorganism dirts Cross contamination between the pollution of embryo caused by contaminating or embryo, and this is simple using novel manufacture craft, it is low in cost.In embryo When tire freezen protective, may make embryo's vertical immersion in liquid nitrogen, with liquid nitrogen fluid change can occur accordingly sink or on It rises, embryo will not be caused to damage since liquid nitrogen is reduced.
Detailed description of the invention
Fig. 1 novel closed-type glass freezing described in the utility model carries the schematic diagram of bar
Fig. 2 novel closed-type glass freezing described in the utility model carries the cross that embryo in bar places embryo's placement end of pipe Schematic cross-section.
Specific embodiment
Utility model will be further described in detail below with reference to the attached drawings and specific embodiments.
As shown in Figure 1 and Figure 2, respectively marked as the freezing closing of 1. new-type of vetrifying carries bar in figure;2. outer tube;3. embryo is put Set pipe;4. isolated tube;5. metal column;3-1. embryo places groove.
A kind of novel closed-type glass freezing described in the utility model carries bar 1, including outer tube 2, embryo place pipe 3, Isolated tube 4, metal column 5;The metal column 5 is placed in 2 bottom of outer tube;The isolated tube 4 is placed in 5 upper layer of metal column; The embryo places pipe 3 and is placed in 4 upper layer of isolated tube, and embryo places 3 top of pipe away from 2 nozzle 1.3cm of outer tube, nozzle sealing Machine is sealed.
Above-mentioned novel closed-type glass freezing carries bar 1, and the outer tube 2 is that open type glassization freezing carries bar, length Degree is 13.2cm, diameter 0.3cm;The metal column 5 is stainless steel metal column, and the length is 1.5cm, diameter 0.2cm;Institute State embryo place 3 diameter of pipe be 0.2cm, length 6.6cm, embryo place 3 one end of pipe be embryo place end, the embryo place end Groove 3-1 is placed for embryo;The embryo places groove 3-1 and places the angle that 3 one end of pipe, one length of beveling is 2cm by embryo It is formed;The isolated tube 4 is intermediate closed pipe, and the length is 2cm, intermediate confining bed length is 1.4cm.
Above-mentioned novel closed-type glass freezing carries bar 1, and the embryo places the nozzle cross section that pipe 3 places end in embryo Angle be greater than 180 °, that is to say, that the part chamfer, the angle that shows is less than on the nozzle cross section that embryo places end 180°。
Use process:Metal column 5 is placed on 2 bottom of outer tube, places into isolated tube 4, embryo is taken out and places pipe 3, Glass freezing liquid is got out, frozen embryo is put into embryo under the microscope and put by operator according to patient requests' frozen embryo It sets in geosynclinal concave slot 3-1, after operator completes the placement of embryo, the novel embryo vitrifying freeze for holding the utility model carries bar embryo Tire places 3 upper end of pipe, and embryo's placement groove 3-1 is placed in downward in outer tube 2, is sealed with sealing machine, finally by novel sealing Enclosed glass freezing carries bar 1 and is placed in progress Embryo freezing preservation in liquid nitrogen storage pipe.
The above embodiments are only used to help understand the utility model, keeps professional and technical personnel in the field real Now or the utility model is used, is not intended to limit the utility model, within the spirit and principle of the utility model, is made Any modification, equivalent substitution, improvement and etc., should be included within the scope of protection of this utility model.

Claims (3)

1. a kind of novel closed-type glass freezing carries bar, which is characterized in that including outer tube (2), embryo place pipe (3), every From pipe (4), metal column (5);The metal column (5) is placed in outer tube (2) bottom;The isolated tube (4) is placed in metal Column (5) upper layer;The embryo places pipe (3) and is placed in isolated tube (4) upper layer, and embryo places pipe (3) top and manages away from outer tube (2) Mouth 1.3cm, nozzle are sealed.
2. novel closed-type glass freezing according to claim 1 carries bar, which is characterized in that the outer tube (2) is Open type glassization freezing carries bar, and the length is 13.2cm, diameter 0.3cm;The metal column (5) is stainless steel metal column, The length is 1.5cm, diameter 0.2cm;The embryo place pipe (3) diameter be 0.2cm, length 6.6cm, embryo places pipe (3) one end is that embryo places end, and it is that embryo places groove (3-1) which, which places end,;The embryo places groove (3-1) by embryo Tire is placed the angle that beveling one length in pipe (3) one end is 2cm and is formed;The isolated tube (4) is intermediate closed pipe, length For 2cm, intermediate confining bed length is 1.4cm.
3. novel closed-type glass freezing according to claim 2 carries bar, which is characterized in that the embryo places pipe (3) angle that the nozzle cross section at end is placed in embryo is greater than 180 °.
CN201820296817.1U 2018-03-05 2018-03-05 A kind of novel closed-type glass freezing load bar Active CN208159973U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201820296817.1U CN208159973U (en) 2018-03-05 2018-03-05 A kind of novel closed-type glass freezing load bar

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201820296817.1U CN208159973U (en) 2018-03-05 2018-03-05 A kind of novel closed-type glass freezing load bar

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CN208159973U true CN208159973U (en) 2018-11-30

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109362706A (en) * 2018-10-17 2019-02-22 北京大学深圳医院 Embryo cryopreservation carries lever apparatus
WO2020147604A1 (en) * 2019-01-18 2020-07-23 上海廪典实业有限公司 Ultralow-temperature freezing and sealing type preservation method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109362706A (en) * 2018-10-17 2019-02-22 北京大学深圳医院 Embryo cryopreservation carries lever apparatus
WO2020147604A1 (en) * 2019-01-18 2020-07-23 上海廪典实业有限公司 Ultralow-temperature freezing and sealing type preservation method

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