CN206902163U - CIK cultivate reagent boxes - Google Patents
CIK cultivate reagent boxes Download PDFInfo
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- CN206902163U CN206902163U CN201720712252.6U CN201720712252U CN206902163U CN 206902163 U CN206902163 U CN 206902163U CN 201720712252 U CN201720712252 U CN 201720712252U CN 206902163 U CN206902163 U CN 206902163U
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- freezing
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- tube seat
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Abstract
It the utility model is related to a kind of cultivate reagent box, especially CIK cultivate reagents box, including hinged box body and lid, also include elastic heat preserving courage, the elastic heat preserving courage is arranged in box body, the center of elastic heat preserving courage is provided with freezing tank, and the surrounding of freezing tank is respectively equipped with culture foundation trench, separation liquid tank, cleans liquid bath and freeze tube seat；The freezing tank places biological ice bag, and the culture foundation trench places medium bottle, and the separation liquid tank places separating liquid bottle, and the cleaning liquid bath places cleaning fluid bottle, and the tube seat that freezes places cell factor cryopreservation tube.CIK cultivate reagents box provided by the utility model is rationally distributed, space utilization is abundant, it is compact-sized, take conveniently, cooling is fast, the cryopreservation tube Cord blood time is long and stably.
Description
Technical field
It the utility model is related to a kind of cultivate reagent box, especially CIK cultivate reagents box.
Background technology
At present, the method for inducing and cultivating of CIK cell is more ripe, due to the reagent that is used in its incubation and auxiliary
Help the factor more, it is necessary to according to unified standard, these reagents accurately to be dispensed according to each plant demand, reagent is made
Box, the solution of complete set is provided for CIK cell culture.Existing CIK kits inner packing design is unreasonable, it is impossible to ensures
Cell factor maintains low temperature in long-distance transport, and this just likely results in factors inactive, has a strong impact on inducing effect；Or do not press
Ratio dispenses each factor, does not accomplish to calculate by everyone every part, causes to waste.
Utility model content
To solve the above problems, the utility model provide one kind can keep maintaining inside cultivate reagent box low-temperature condition,
Rationally distributed CIK cultivate reagent boxes, concrete technical scheme are：
CIK cultivate reagent boxes, including hinged box body and lid, in addition to elastic heat preserving courage, the elasticity are protected
Warm courage is arranged in box body, and the center of elastic heat preserving courage is provided with freezing tank, and the surrounding of freezing tank is respectively equipped with culture foundation trench, separation
Liquid bath, clean liquid bath and freeze tube seat；The freezing tank places biological ice bag, and the culture foundation trench places medium bottle, described
Separation liquid tank places separating liquid bottle, and the cleaning liquid bath places cleaning fluid bottle, and the tube seat that freezes places cell factor cryopreservation tube.
Preferably, the freezing tank is rectangular recess, and the top of freezing tank is covered equipped with freezing, the separation liquid tank and cleaning
Liquid bath is square groove, and the tube seat that freezes is circular groove, and the culture foundation trench is square groove；In the culture foundation trench
500ml medium bottles are placed, the separation liquid tank places 100ml separating liquid bottles, and the cleaning liquid bath places 250ml cleaning fluids
Bottle.
Wherein, the culture foundation trench is provided with two, is respectively positioned at the both ends of freezing tank positioned at the left and right sides of box body；Institute
State and freeze tube seat no less than three, freeze tube seat and be arranged side by side, freeze tube seat positioned at the centre of two culture foundation trenches, and freezing
The side of groove；The separation liquid tank and cleaning liquid bath are arranged side by side, and are respectively positioned on the centre of two culture foundation trenches, and in freezing tank
Opposite side.
Further, the freezing tank and freeze and be equipped with through hole between tube seat.
Wherein, the freezing tank and the through hole frozen between tube seat are no less than two, and the bottom of the freezing tank is with freezing
The bottom of tube seat is connected by through hole.
Preferably, the freezing tank and freeze conduction cooling plate be housed between tube seat, conduction cooling plate respectively with freezing tank and cryopreservation tube
Groove connects.
Preferably, connect band is housed, lid passes through snap lock with box body between the lid and box body.
Preferably, the top of the lid is equipped with handle and release, and the inner side of the lid is equipped with information kit.
The proportioning of the reagents such as culture medium, cleaning fluid and separating liquid has been subjected to optimization, and great convenience is brought to operator.This
Outside, kit makes full use of space using rational layout so that and it is compact-sized, take conveniently, take into account low-temperature transport.
CIK cell culture comprises the following steps：
1. the hematology aliquot of acquisition is subjected to just point removal blood plasma in centrifuge tube；
2. add appropriate cleaning fluid in the erythrocyte sedimentation rate for removing blood plasma forms sediment to be diluted；
3. appropriate cell separating liquid is added in empty centrifuge tube；
It is slowly added to 4. the erythrocyte sedimentation rate of dilution forms sediment according to set ratio in the centrifuge tube equipped with cell separating liquid；
5. the centrifuge tube added by more than is centrifuged, cellular layer is drawn in new centrifuge tube；
6. centrifuge washing is carried out for several times to the cell of acquisition with cleaning fluid；
Cultivated 7. the cell washed is resuspended to add in blake bottle with culture medium；
8. in cell cultivation process, propagation and maturation that the cell culture factor promotes cell are sequentially added.
The utility model has the advantages that compared with prior art：
CIK cultivate reagents box provided by the utility model is rationally distributed, space utilization is abundant, it is compact-sized, take conveniently,
Cooling is fast, the cryopreservation tube Cord blood time is long and stably.
Brief description of the drawings
Fig. 1 is the structural representation of the utility model open mode；
Fig. 2 is the structural representation of the utility model closed mode.
Embodiment
In conjunction with accompanying drawing, the utility model is described in further detail.
Embodiment 1
As depicted in figs. 1 and 2, CIK cultivate reagents box, including hinged box body and lid, lid and box body it
Between connect band 7 is housed, lid passes through snap lock with box body.The top of lid is equipped with handle 8 and release 9, the inner side of lid
Equipped with information kit 1.
Also include elastic heat preserving courage, the elastic heat preserving courage is arranged in box body, and the center of elastic heat preserving courage is provided with freezing
Groove 6, the surrounding of freezing tank 6 are respectively equipped with culture foundation trench 5, separation liquid tank 2, cleaning liquid bath 3 and freeze tube seat 4.
Freezing tank 6 is rectangular recess, and the top of freezing tank 6 is covered equipped with freezing, and freezing tank 6 places biological ice bag.
Cultivate foundation trench 5 and be provided with two, be direction groove, be respectively positioned at the two of freezing tank 6 positioned at the left and right sides of box body
End, culture foundation trench 5 place 500ml medium bottles.
Separation liquid tank 2 is square groove, places 100ml separating liquid bottles, cleaning liquid bath 3 is also direction groove, places 250ml
Cleaning fluid bottle.Separation liquid tank 2 and cleaning liquid bath 3 are arranged side by side, and are respectively positioned on the centre of two culture foundation trenches 5, and in freezing tank 6
Opposite side.
It is circular groove to freeze tube seat 4, provided with three, freezes tube seat 4 and is arranged side by side, and freezes tube seat 4 positioned at two cultures
The centre of foundation trench 5, and in the side of freezing tank 6, placement cell factor cryopreservation tube.
Freezing tank 6 and freeze and be equipped with through hole between tube seat 4.Freezing tank 6 and the through hole frozen between tube seat 4 are no less than two
Individual, the bottom of the freezing tank 6 is connected with freezing the bottom of tube seat 4 by through hole.
It is sorting placement, rationally distributed, easy to use.The proportioning of various reagents has been subjected to optimization, and once culture uses enough,
Reduce and waste, it is cost-effective.Cultivate and 500ml medium bottles are placed in foundation trench 5,500ml culture mediums are housed in medium bottle.Separation
Liquid bath 2 places 100ml separating liquid bottles, and 80ml separating liquids are housed in liquid bottle of classifying.Clean liquid bath 3 and place 250ml cleaning fluid bottles, clearly
200ml cleaning fluids are housed in bottle for handling liquid toilet or cosmetic substance.
Freezing lid makes the high insulating effect of freezing tank 6, extends the usage time of biological ice bag, and guarantee freezes tube seat 4 in length
2-8 DEG C is maintained in the transportation of way.
Freezing tank 6 and the through hole frozen between tube seat 4 facilitate cold air to keep cryopreservation tube to be in low into freezing in tube seat 4
Temperature state.The bottom of freezing tank 6 is connected with freezing the bottom of tube seat 4 by through hole, and the temperature that guarantee is frozen in tube seat 4 is uniform.
Connect band 7 controls the angle that lid is opened.
Release 9 is used for the situation and origin and place of acceptance for filling in cell factor.
Embodiment 2
On the basis of above-described embodiment, freezing tank 6 and freeze conduction cooling plate be housed between tube seat 4, conduction cooling plate respectively with it is cold
Freeze groove 6 to connect with tube seat 4 is frozen.
Conduction cooling plate is made using aluminium or copper, and freezing tank 6 is connected with tube seat 4 is frozen, convenient to freezing freezing in tube seat 4
Pipe carries out fast cooling, while cryopreservation tube is effectively ensured and maintains low-temperature condition, makes to keep low temperature during long-distance transport, prevents thin
Intracellular cytokine inactivates.
Claims (8)
1.CIK cultivate reagent boxes, including hinged box body and lid, it is characterised in that also including elastic heat preserving courage, institute
Elastic heat preserving courage is stated in box body, the center of elastic heat preserving courage is provided with freezing tank（6）, freezing tank（6）Surrounding set respectively
There is culture foundation trench（5）, separation liquid tank（2）, cleaning liquid bath（3）With freeze tube seat（4）；The freezing tank（6）Biological ice bag is placed,
The culture foundation trench（5）Place medium bottle, the separation liquid tank（2）Place separating liquid bottle, the cleaning liquid bath（3）Place clear
Bottle for handling liquid toilet or cosmetic substance, it is described to freeze tube seat（4）Place cell factor cryopreservation tube.
2. CIK cultivate reagents box according to claim 1, it is characterised in that the freezing tank（6）It is cold for rectangular recess
Freeze groove（6）Top equipped with freezing cover, the separation liquid tank（2）With cleaning liquid bath（3）It is square groove, it is described to freeze tube seat
（4）For circular groove, the culture foundation trench（5）For square groove；The culture foundation trench（5）Interior placement 500ml medium bottles, institute
State separation liquid tank（2）Place 100ml separating liquid bottles, the cleaning liquid bath（3）Place 250ml cleaning fluid bottles.
3. CIK cultivate reagents box according to claim 2, it is characterised in that the culture foundation trench（5）Provided with two, point
Not Wei Yu box body the left and right sides i.e. be located at freezing tank（6）Both ends；It is described to freeze tube seat（4）No less than three, tube seat is frozen
（4）It is arranged side by side, freezes tube seat（4）Positioned at two culture foundation trenches（5）Centre, and in freezing tank（6）Side；The separation
Liquid bath（2）With cleaning liquid bath（3）It is arranged side by side, is respectively positioned on two culture foundation trenches（5）Centre, and in freezing tank（6）It is another
Side.
4. CIK cultivate reagents box according to claim 3, it is characterised in that the freezing tank（6）With freezing tube seat（4）
Between be equipped with through hole.
5. CIK cultivate reagents box according to claim 4, it is characterised in that the freezing tank（6）With freezing tube seat（4）
Between through hole be no less than two, the freezing tank（6）Bottom and freeze tube seat（4）Bottom connected by through hole.
6. CIK cultivate reagents box according to claim 3, it is characterised in that the freezing tank（6）With freezing tube seat（4）
Between conduction cooling plate is housed, conduction cooling plate respectively with freezing tank（6）With freeze tube seat（4）Connection.
7. CIK cultivate reagents box according to claim 1, it is characterised in that equipped with connection between the lid and box body
Band（7）, lid and box body pass through snap lock.
8. CIK cultivate reagents box according to claim 1, it is characterised in that the top of the lid is equipped with handle（8）With
Release（9）, the inner side of the lid is equipped with information kit（1）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720712252.6U CN206902163U (en) | 2017-06-19 | 2017-06-19 | CIK cultivate reagent boxes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720712252.6U CN206902163U (en) | 2017-06-19 | 2017-06-19 | CIK cultivate reagent boxes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206902163U true CN206902163U (en) | 2018-01-19 |
Family
ID=61285272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720712252.6U Active CN206902163U (en) | 2017-06-19 | 2017-06-19 | CIK cultivate reagent boxes |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206902163U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111547354A (en) * | 2020-05-11 | 2020-08-18 | 南京翼飞雪生物科技有限公司 | RNA extraction kit and extraction method |
-
2017
- 2017-06-19 CN CN201720712252.6U patent/CN206902163U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111547354A (en) * | 2020-05-11 | 2020-08-18 | 南京翼飞雪生物科技有限公司 | RNA extraction kit and extraction method |
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