CN218594852U - Simple and practical cell cryopreservation box - Google Patents

Abstract

The utility model relates to a cell freezes deposits technical field, and discloses a simple and convenient practical cell freezes deposits box, including lid, box body, cell freezes deposits the pipe and the cell freezes deposits the pipe holds the chamber, the lid block is in the top of box body, the cell freezes deposits the pipe and holds the chamber and set up to a plurality ofly, the cell freezes deposits the pipe and pegs graft and holds the intracavity at cell freezes deposits the pipe, this utility model discloses a cell freezes deposits the pipe and holds chamber bottom to the lid internal surface highly be equal to the cell freezes deposits the pipe height, consequently when putting into cell freezes deposits the pipe, can jack-up the cylinder piece, cylinder piece block on the lid is connected simultaneously, convenient dismantlement is changed, has avoided depositing and has frozen the box and have not had the method visual display on its quantity and position, especially different generations or when different kind of cell put into together and freezes deposits the box, very easily obscure, bring inconvenient problem for subsequent operation, also avoided the cell to freeze and deposit the box in the refrigerator after, its rotary cover is hardly opened, uses inconveniently, is unfavorable for the problem of the transfer of cell.

Description

Simple and practical cell cryopreservation box

Technical Field

The utility model belongs to the technical field of the cell cryopreserving, specifically be a simple and convenient practical cell cryopreserving box.

Background

With the development of life science and technology, stem cell therapy as an emerging technology has made remarkable progress and is increasingly accepted by people. The primary step of stem cell therapy is to separate and obtain cells from tissues such as umbilical cord, placenta and fat, etc., and the cells are used for treating diseases after being cultured and expanded in vitro. After the stem cells are expanded and cultured, the stem cells need to be stored, in the process of storage, the activity and the biological activity of the cells determine the clinical effect of the cells in treating diseases, the stem cells are one of the core links in the processes of production and storage of the cells, the freezing storage of the cells needs to follow the principle of slow freezing, and the aim is to prevent the cells from being damaged due to the formation of ice crystals in the cells in the freezing storage process. The traditional operation is that the cells are sequentially placed at 4 ℃ and-20 ℃ for different times, and finally placed at-80 ℃ for 24 hours, and then the frozen cells are transferred to a liquid nitrogen tank for long-term storage. In contrast, devices that lower the temperature gradually, such as programmed cell cryopreservation cassettes, are now commonly used, and such freezing vessels can achieve a cooling rate of-1 ℃/minute, and are suitable for low temperature cryopreservation of cells. However, programmed cell cryopreservation boxes need to use isopropanol as a carrier for heat storage and dissipation, the isopropanol belongs to a toxic and harmful reagent and is easy to volatilize, and the isopropanol needs to be replaced after being used for a certain number of times, so that the programmed cell cryopreservation boxes not only can pollute the environment, but also can have a certain toxic effect on experiment operators after being used for a long time.

Present deposit freeze box does not have visual display in its quantity and position, especially different generations or different kind cells put into together and freeze when depositing the box, very easily obscure, bring inconvenience for follow-up operation, in addition, the outside structure design that programmed cell freezes deposits the box is general revolving cap design, directly freezes in low temperature refrigerator when the gradient box that cools down freezes the back of depositing, its revolving cap is difficult to open, and it is inconvenient to use, is unfavorable for the transfer of cell, consequently, proposes a simple and convenient practical cell here and deposits and freezes the box.

SUMMERY OF THE UTILITY MODEL

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a simple and convenient practical cell cryopreserved box, freezes including lid, box body, cell and deposits the pipe and the cell cryopreserved pipe holds the chamber, the lid block is in the top of box body, the cell cryopreserved pipe holds the chamber and sets up to a plurality ofly, and even distribution is in the bottom of box body, the cell cryopreserved pipe is pegged graft and is being frozen at the cell and deposit the pipe and hold the intracavity, and the cell cryopreserved pipe holds highly being less than the cell height of depositing the pipe in chamber, still be provided with the cylinder that can reciprocate on the box cover.

Further, a plurality of through-holes have been seted up on the lid, and the position of through-hole and cell deposit freeze the position that holds the chamber and correspond, the draw-in groove has been seted up at the both ends at through-hole top, the both ends fixed connection fixture block at cylinder top, and the fixture block is in the draw-in groove.

Further, a limiting groove is formed in the tops of the two sides of the box body, limiting blocks are fixedly connected to the bottoms of the two ends of the box cover, and the limiting blocks are clamped in the limiting groove.

Further, the height from the bottom of the cell freezing storage tube accommodating cavity to the bottom of the box cover is equal to the height of the cell freezing storage tube.

Further, the height of cylinder is greater than the height of lid, works as when the top of cell cryopreserving pipe supports the bottom at the cylinder, the cylinder runs through the through-hole and extends to outside the lid.

Furthermore, the box body and the box cover are both made of EPS foam or EPP foam.

Compared with the prior art, the beneficial effects of the utility model are that: it stores the pipe height to be equated with the cell cryopreserving to highly being equal to of chamber bottom to lid internal surface through the cell cryopreserving pipe, consequently when putting into the cell cryopreserving pipe, can be with cylinder piece jack-up, cylinder piece block on the lid is connected simultaneously, the change is conveniently dismantled, avoided depositing and freeze the box and fail visual display in its quantity and position, especially different generations or when different kind cells put into together and freeze the box, very easily obscure, bring inconvenient problem for subsequent operation, also avoided the cell cryopreserving box freeze the back of depositing in the refrigerator, its swivel cap is hardly opened, and is inconvenient to use, be unfavorable for the problem of the transfer of cell.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:

FIG. 1 is a schematic view of the main sectional structure of the present invention;

FIG. 2 is a schematic view of the cylinder engaging structure of the present invention;

fig. 3 is a schematic top view of the lid of the present invention;

FIG. 4 is a top view of the box body with schematic structure of the present invention

In the figure: 1. a box cover; 2. a box body; 3. a cylinder; 4. freezing and storing the cells; 5. the cell freezing tube accommodating cavity; 6. a limiting groove; 7. a limiting block; 8. a through hole; 9. a card slot; 10. and (6) clamping blocks.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments; based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

As shown in fig. 1-4, the utility model discloses a lid 1, box body 2, cell cryopreserving pipe 4 and cell cryopreserving pipe hold chamber 5, 1 block of lid is in the top of box body 2, and cell cryopreserving pipe holds chamber 5 and sets up to a plurality ofly, and even distribution is in the bottom of box body 2, and cell cryopreserving pipe 4 pegs graft in cell cryopreserving pipe holds chamber 5, and cell cryopreserving pipe holds chamber 5 highly be less than cell cryopreserving pipe 4's height, still be provided with cylinder 3 that can reciprocate on the lid 1.

As shown in fig. 1-2, a plurality of through holes 8 are formed in the box cover 1, the positions of the through holes 8 correspond to the positions of the cell freezing accommodating cavities 5, clamping grooves 9 are formed in the two ends of the top of each through hole 8, clamping blocks 10 are fixedly connected to the two ends of the top of the cylinder 3, and the clamping blocks 10 are clamped in the clamping grooves 9, so that the cylinder 3 can move up and down in the through holes and can not fall off when the top of the cell freezing accommodating tube 4 is not supported.

As shown in figure 1, the top of the both sides of box body 2 has been seted up spacing groove 6, and the bottom fixedly connected with stopper 7 at 1 both ends of lid, and stopper 7 block is in spacing groove 6 to make the cell cryopreserving box conveniently open the lid after freezing.

As shown in figure 1, the height from the bottom of the cell freezing tube accommodating cavity 5 to the bottom of the box cover 1 is equal to the height of the cell freezing tube 4, so that the top of the cell freezing tube 4 is conveniently abutted against the bottom of the cylinder 3.

As shown in figure 1, the height that highly is greater than lid 1 of cylinder 3, when the top of cell cryopreserving pipe 4 supported the bottom at cylinder 3, cylinder 3 run through hole 8 and extend to outside lid 1, the cylinder piece on the lid of being convenient for can dismantle the change, also can change different colours as required, when different generations or kind cell cryopreserved, available different colours distinguish, avoid obscuring, the follow-up operation of being convenient for.

As shown in figure 1, the box body 2 and the box cover 1 are both made of EPS foam or EPP foam, so that the freezing storage box is provided with good heat insulation performance and is safe to use.

Example 1

The umbilical cord mesenchymal stem cells are cryopreserved by using two different cell cryopreservation boxes (namely an isopropanol cryopreservation box and the EPS material cryopreservation box), and the method comprises the following specific operation steps:

1. cell passage

1.1 taking out the frozen stock solution, PBS, pancreatin and stem cell culture solution from the refrigerator. The pancreatin and the stem cell culture solution are put in a water bath kettle for pre-warming.

1.2 taking out the T175 culture flask for culturing the umbilical cord mesenchymal stem cells in the culture box, observing the cell confluence rate to about 90% under a microscope, discarding the waste liquid in the culture flask, adding PBS solution according to the amount of 10mL per flask, cleaning the cells, and discarding the PBS waste liquid.

1.3 adding pancreatin in an amount of 5mL per bottle, gently shaking to infiltrate cells into the pancreatin, and placing the cells into an incubator for 1-2 min.

1.4 gently shake the flask to see if all cells have fallen off, and add 3ml of culture medium to stop digestion.

1.5 taking a 50mL centrifuge tube, transferring the cell suspension into the centrifuge tube, taking 100uL of cell suspension for counting, centrifuging the rest cell suspension at 2000rpm for 5min.

1.6 according to the cell counting results, the cell culture medium was added to adjust the cell concentration to 5X 106 cells/mL. 1mL of each tube was filled into a cell cryopreservation tube. The cell freezing tubes were placed in different cell freezing boxes and placed in a-80 ℃ freezer.

And 1.7 taking out the frozen cells every other day, recovering, counting the cells and calculating the cell survival rate.

Cells were cryopreserved in 6 groups for each type as shown in Table 1, and cells were cryopreserved in 5X 106 groups for each group, and finally subjected to statistical analysis. As shown in table 1:

it is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. The utility model provides a simple and convenient practical cell cryopreserving box, includes lid (1), box body (2), cell cryopreserving pipe (4) and cell cryopreserving pipe and holds chamber (5), its characterized in that: lid (1) block is in the top of box body (2), the cell cryopreserved pipe holds chamber (5) and sets up to a plurality ofly, and even distribution is in the bottom of box body (2), the cell cryopreserved pipe (4) are pegged graft in cell cryopreserved pipe holds chamber (5), and the cell cryopreserved pipe holds the height that highly is less than cell cryopreserved pipe (4) in chamber (5), still be provided with cylinder (3) that can reciprocate on lid (1).

2. The practical and easy cell freezing kit according to claim 1, wherein: a plurality of through holes (8) are formed in the box cover (1), the positions of the through holes (8) correspond to the positions of the cell freezing and storing pipe accommodating cavities (5), clamping grooves (9) are formed in the two ends of the top of each through hole (8), clamping blocks (10) are fixedly connected to the two ends of the top of the cylinder (3), and the clamping blocks (10) are clamped in the clamping grooves (9).

3. The practical and easy cell freezing kit according to claim 1, wherein: spacing groove (6) have been seted up at the top of the both sides of box body (2), the bottom fixedly connected with stopper (7) at lid (1) both ends, and stopper (7) block is in spacing groove (6).

4. The practical and easy cell freezing kit according to claim 1, wherein: the height from the bottom of the cell freezing and storing tube accommodating cavity (5) to the bottom of the box cover (1) is equal to that of the cell freezing and storing tube (4).

5. The practical and easy cell freezing kit according to claim 1, wherein: the height of cylinder (3) is greater than the height of lid (1), works as when the top of cell cryopreserving pipe (4) supports the bottom at cylinder (3), and cylinder (3) run through-hole (8) and extend to outside lid (1).

6. The practical and easy cell freezing kit according to claim 1, wherein: the box body (2) and the box cover (1) are both made of EPS foam or EPP foam.

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