CN209995212U - device for cryopreservation of large-volume biological samples - Google Patents
device for cryopreservation of large-volume biological samples Download PDFInfo
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- CN209995212U CN209995212U CN201920535459.XU CN201920535459U CN209995212U CN 209995212 U CN209995212 U CN 209995212U CN 201920535459 U CN201920535459 U CN 201920535459U CN 209995212 U CN209995212 U CN 209995212U
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Abstract
The utility model belongs to the biomedical field discloses kind of bulky biological sample cryo-preservation's device, the device includes support piece and centers on the polytetrafluoroethylene pipe outside the support piece, biological sample adds protective agent and then pours into after the preliminary treatment when bulky biological sample cryo-preservation in the polytetrafluoroethylene pipe in the device, with the device cryopreservation, the water bath rewarming when treating needs, the successful realization of cryo-preservation device the bulky biological sample cryopreservation under the low concentration protective agent effect is kind long-term safety, reliable and stable, simple effectual device of preserving biological sample, can obtain the biological sample that the micro-structure is complete, cell vitality is strong, biological function is excellent through the device.
Description
Technical Field
The utility model belongs to the biomedical field, concretely relates to device that kind bulky biological sample cryogenics were preserved.
Background
The low-temperature preservation of the biological material means that the biological material is cooled to low temperature by adopting a specific method and is preserved for a long time; if desired, the biomaterial can be rewarmed to physiological temperature in a particular manner and still retain sufficient activity. With the development of modern biomedicine, the function of low-temperature preservation is increasingly prominent, and the method has great significance for the cryopreservation of large-volume biological samples such as tissues, organs and the like.
Typical cryopreservation mainly comprises five steps: adding protective agent, cooling, long-term storage, rewarming, and removing protective agent. During cryopreservation, biological materials are inevitably subjected to various damages, and the main initiating factors include: change of osmotic pressure, growth of ice crystals, temperature gradient inside the sample in the processes of temperature reduction and rewarming, devitrification and recrystallization in the process of rewarming and the like. Therefore, low solution concentration and rapid and uniform cooling and rewarming process are important conditions for successful preservation of biological samples.
The current solution for cryopreservation of large-sized biological samples, such as blood vessels, is to process the samples and place them in cryovial tubes, which will curl and bend after freezing, which will cause damage to the structure, mechanical and biological properties of the biological samples. In addition, the existing preservation technology mostly adopts a method of gradually adding a protective agent, uses a programmed cooling process, adopts a mode of shaking and passively rewarming in a constant-temperature water bath, lasts for a long time, is complex to operate, and seriously influences the preservation effect of the large-volume biological sample.
Due to the macroscopic size of the low-temperature freezing storage tube and the conduction mode of heat from outside to inside, the heat transmission speed is limited, the temperature difference of each position inside the biological material sample in the temperature reduction and rewarming process is large, the temperature gradient inside and outside the sample is large, large thermal stress is generated, and fracture and microcrack are easily caused. The fast rate of rewarming can cause serious devitrification and recrystallization, thereby seriously affecting the preservation effect and survival rate; at the same time, high concentrations of cryoprotectants may lead to uncontrolled osmotic damage and biochemical toxicity, which is fatal to the biological material.
SUMMERY OF THE UTILITY MODEL
In view of the above, the present invention aims to provide kinds of devices for cryopreservation of large-volume biological samples, which are suitable for long-term and efficient storage of large-volume biological samples such as cells, cell hydrogel constructs, and micro-tissues.
For realizing the purpose of the utility model, the utility model adopts the following technical scheme:
A device for cryopreservation of large-volume biological sample comprises th support member and PTFE tube surrounding th support member.
The device that the low temperature was preserved is different from freezing containers such as the conventional low temperature cryopreserving pipe, cryopreserved bag, chooses polytetrafluoroethylene pipe for use as cryopreserved container for use, and its good pliability of make full use of, characteristics such as ductility and biocompatibility have carried out "little metaplasia" to it under the prerequisite that does not change biological sample characteristic dimension, when guaranteeing heat transfer effect, has enlarged the cryopreserved volume of sample to reach the target of cryopreserving bulky biological sample.
The utility model discloses can select the polytetrafluoroethylene pipe of suitable size to freeze the biological sample and deposit according to the concrete kind of biological sample. When the biological sample is an adipose-derived stem cell, a stem cell hydrogel construct or a stem cell hydrogel construct with a core-shell structure, the polytetrafluoroethylene tube specification is 1.2mm inner diameter x 1.6mm outer diameter; when the biological sample is stem cell hydrogel fiber with a core-shell structure, the specification of the polytetrafluoroethylene tube is 2mm inner diameter multiplied by 2.4mm outer diameter; when the biological sample is a blood vessel tissue, the polytetrafluoroethylene tube specification is 3mm inner diameter multiplied by 3.4mm outer diameter.
Preferably, the supporting member is a rectangular supporting plate.
Preferably, the device of the present invention further comprises a second supporting member for fixing the th supporting member.
Preferably, the second strutting piece is a rectangular support frame with a plurality of grooves arranged in pairs from top to bottom, and the th strutting piece is fixed on the second strutting piece through the grooves arranged in pairs from top to bottom, namely, every on the second strutting piece can fix th strutting pieces to the grooves correspondingly arranged from top to bottom.
As can be understood by those skilled in the art, the second supporting member upper groove of the present invention can also be disposed on both sides of the rectangular supporting frame, as long as the th supporting member can be fixed.
As can be understood by those skilled in the art, the th supporting member of the present invention may also have the same structure as the second supporting member, and a plurality of pairs of rectangular supporting frames are arranged in pairs for the upper and lower sides or both sides, so long as it can ensure that the polytetrafluoroethylene tube surrounds the outer surface of the polytetrafluoroethylene tube.
As can be understood by those skilled in the art, those skilled in the art can set the size of the rectangular support frame and the number of pairs of grooves on the rectangular support frame according to the volume and type of the biological sample to be stored at low temperature. Such as two, three, four, five, six, etc.
The utility model discloses in support piece with the second support piece can be the same material, also can be different materials.
Preferably, the th support piece and the second support piece are made of organic glass materials.
Preferably, the device of the utility model also comprises a water bath rewarming container which can be used for rewarming the water bath after freezing.
Preferably, the water bath rewarming container is a glass beaker.
Preferably, the device of the utility model also comprises an electromagnetic coil surrounding the water bath rewarming container, when the biological sample is frozen, a proper amount of magnetic nanoparticles are added into the biological sample (suspension) for balancing, when rewarming is needed, an electromagnetic induction technology is adopted, the biological sample is heated in a whole volume and in multiple dimensions, and then the constant temperature water bath is combined, so that the large-volume biological sample is rapidly and uniformly rewarmed.
The type and concentration of the magnetic nanoparticles may vary from biological sample to biological sample. Preferably, the magnetic nanoparticles are Fe3O4And (3) nanoparticles. Fe3O4The nano-particles have the advantages of good biocompatibility, remarkable magnetocaloric effect, FDA certification and the like. Fe3O4The electromagnetic rewarming can be better realized by adding the nano particles.
When will realizing different kinds of biological sample rewarming simultaneously, combine the foretell add the magnetism nanoparticle of different kinds and concentration in to different middle-class biological samples, then pour into different kinds of biological samples respectively the polytetrafluoroethylene tube of the aforesaid different units of device in, can realize in the different space heating models under with electromagnetic field to realize different kinds of biological sample rewarming simultaneously.
Preferably, in the method for low-temperature storage according to the present invention, the current in the electromagnetic coil is 15A.
According to the above technical scheme, the utility model provides a device that kind bulky biological sample cryo-preservation, the device includes support piece and centers on the polytetrafluoroethylene pipe outside support piece, when bulky biological sample cryo-preservation, biological sample adds protective agent after the preliminary treatment then pours into in the polytetrafluoroethylene pipe among the device, with the device cryopreservation, water bath rewarming when waiting to need, the utility model discloses the successful bulky biological sample cryopreservation that has realized under the low concentration protective agent effect of cryo-preservation device is kinds of long-term safety, reliable and stable, simple effectual device of preserving biological sample, can obtain the biological sample that the microstructure is complete, the cell vitality is strong, biological function is excellent through the device, compare with the device of current cryo-cryopreservation, the utility model discloses the device has of following advantage at least:
1. the utility model discloses a polytetrafluoroethylene pipe freezes as biological sample and deposits the container, has enlarged the sample volume but does not harm the heat transfer to the freezing of bulky biological sample has been realized and has been deposited.
2. The utility model discloses the water bath rewarming container periphery is around solenoid, adopts electromagnetism rewarming, has the space heating effect to biological sample, and the constant temperature water bath that recombines can realize quick even rewarming to avoid rewarming process to produce devitrification and recrystallization, and can effectively reduce the mechanical damage that factors such as thermal stress that the rewarming process causes led to cause, improved the survival rate that biological sample freezes to be deposited.
3. Under the combined action of the advantages, the devitrification and recrystallization in the cooling and rewarming processes can be effectively inhibited, so that the use concentration of the low-temperature protective agent is reduced, the osmotic damage and the biochemical toxicity are avoided, and the survival rate of the frozen biological sample is improved.
4. Different types and concentrations of biological samples can be injected into the polytetrafluoroethylene tube units outside different th supporting pieces, and by combining the magnetic nanoparticles with different types and concentrations, different types of biological samples can be frozen and rewarming simultaneously under the same electromagnetic field, so that automatic control and integration are easy to realize.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below.
FIG. 1 is a schematic view of a cryopreservation apparatus according to embodiment of the present invention;
FIG. 2: the second embodiment of the present invention provides a schematic view of a low temperature storage device;
FIG. 3: the third embodiment of the present invention provides a schematic view of a cryopreservation apparatus;
in each figure, 1 is an th supporting piece (a rectangular supporting plate), 2 is a polytetrafluoroethylene tube, 3 is a second supporting piece (a rectangular supporting frame), 4 is a water bath rewarming container (a glass beaker), 5 is an electromagnetic coil, and 6 is a groove.
Detailed Description
The technical solution in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only partial embodiments of , but not all embodiments.
Example 1
As shown in FIG. 1, the utility model provides a device that kind bulky biological sample cryogenics were preserved, wherein, 1 is the rectangle backup pad, 2 is the polytetrafluoroethylene pipe 2 centers on outside the rectangle backup pad, can follow rectangle backup pad bottom to the top in proper order around, also can follow rectangle backup pad top to the bottom in proper order around.
Example 2
As shown in FIG. 2, the utility model provides a device that kind bulky biological sample cryogenics were preserved, wherein, 1 is the rectangle backup pad, 2 is the polytetrafluoroethylene pipe, 3 is the rectangle carriage that sets up four pairs of recesses from top to bottom in pairs, polytetrafluoroethylene pipe 2 surrounds and forms units around 1 appearance of rectangle backup pad, four units can assemble and form containers wholly, every can fix winding polytetrafluoroethylene pipe rectangle backup pads to the recess that corresponds the setting from top to bottom on the rectangle carriage promptly, four rectangle backup pads can be fixed to four pairs of recesses on the rectangle carriage, four rectangle backup pads and rectangle carriage assembly form containers wholly, rectangle backup pad and rectangle carriage are the organic glass material.
When the biological sample is stored at low temperature, the biological sample is pretreated, then a protective agent is added, then the biological sample is injected into a polytetrafluoroethylene tube surrounding the rectangular support plate and placed in liquid nitrogen for freezing and storage, and when needed, the biological sample is taken out of the liquid nitrogen and rewarming in a water bath.
In this embodiment, biological samples can be injected into the PTFE tubes surrounded by the four rectangular support plates on the rectangular support frame to ensure the low temperature preservation of large-volume biological samples, or four different biological samples can be injected to ensure the simultaneous freezing and rewarming of different types of biological samples.
Example 3
As shown in FIG. 3, the utility model provides a device that kind bulky biological sample cryo-preservation, wherein, 1 is the rectangle backup pad, 2 is the polytetrafluoroethylene pipe, 3 is the rectangle carriage that sets up four pairs of recesses from top to bottom in pairs, 4 are glass beaker, 5 are solenoid coil, polytetrafluoroethylene pipe 2 centers on and forms units around 1 outer appearance of rectangle backup pad, four pairs of recesses on the rectangle carriage 3 can fix four rectangle backup pads (four units), four rectangle backup pads and rectangle carriage assembly form containers wholly, can place inside glass beaker 4, solenoid coil 5 centers on outside glass beaker 4.
When the biological sample is stored at low temperature, the biological sample is pretreated, then protective agent is added, then magnetic nanoparticles are added, then the biological sample is injected into a polytetrafluoroethylene tube surrounding the rectangular support plate and placed in liquid nitrogen for freezing storage, and when required, the biological sample is taken out from the liquid nitrogen and placed in a glass beaker surrounding an electromagnetic coil in a water bath, and simultaneously the electromagnetic coil is introduced with current for rewarming.
In this embodiment, four different biological samples can be injected into the teflon tubes surrounding the four rectangular support plates on the rectangular support frame, so as to ensure that different types of biological samples are frozen and rewarming at the same time.
Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention.
Claims (7)
- The device for cryopreservation of large-volume biological samples is characterized by comprising a -th support member (1), a polytetrafluoroethylene tube (2) surrounding the -th support member, and a second support member (3) for fixing the -th support member (1).
- 2. The device according to claim 1, characterized in that it further comprises a rewarming container (4) for the water bath.
- 3. The device according to claim 2, further comprising an electromagnetic coil (5) surrounding the rewarming reservoir (4) of the water bath.
- 4. The device according to claim 1, wherein the polytetrafluoroethylene tube (2) gauge is 1.2mm inner diameter x 1.6mm outer diameter, 2mm inner diameter x 2.4mm outer diameter, or 3mm inner diameter x 3.4mm outer diameter.
- 5. The device according to claim 1, characterized in that said th support member (1) is a rectangular support plate.
- 6. The device according to claim 1, wherein the second supporting member (3) is a rectangular supporting frame with a plurality of pairs of grooves (6) arranged in pairs, and the th supporting member (1) is fixed on the second supporting member (3) through the pairs of grooves (6).
- 7. The device according to claim 2, characterized in that the rewarming container (4) is a glass beaker.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920535459.XU CN209995212U (en) | 2019-04-17 | 2019-04-17 | device for cryopreservation of large-volume biological samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920535459.XU CN209995212U (en) | 2019-04-17 | 2019-04-17 | device for cryopreservation of large-volume biological samples |
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| CN209995212U true CN209995212U (en) | 2020-01-31 |
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| CN201920535459.XU Active CN209995212U (en) | 2019-04-17 | 2019-04-17 | device for cryopreservation of large-volume biological samples |
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