CN212589916U - Trace sperm cryopreservation carrier - Google Patents
Trace sperm cryopreservation carrier Download PDFInfo
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- CN212589916U CN212589916U CN202020913835.7U CN202020913835U CN212589916U CN 212589916 U CN212589916 U CN 212589916U CN 202020913835 U CN202020913835 U CN 202020913835U CN 212589916 U CN212589916 U CN 212589916U
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Abstract
The utility model relates to a cell cryopreservation carrier especially relates to a micro sperm cryopreservation carrier. The utility model discloses a carrier is preserved in freezing of trace sperm, including the sperm straw, the sperm straw includes handle and thin slice, the front end of thin slice is opened has the through-hole that is used for forming the sample film. The utility model discloses a carrier is preserved in freezing of trace sperm is easy operation not only, can improve the refrigeration effect of sample moreover.
Description
Technical Field
The utility model relates to a sperm cryopreservation carrier especially relates to a micro sperm cryopreservation carrier.
Background
The establishment of the human sperm bank is now an important component of the human assisted reproductive technology, and the mature sperm freezing technology is the basis for the establishment of the sperm bank. The traditional sperm preservation method is a slow freezing method taking glycerol as a protective agent in the 40 s, and is improved and perfected later, so far. The slow freezing procedure is not suitable for cryopreservation of small or trace sperm samples (severe oligosperm samples or transtesticular, epididymal and sub-dermal epididymal sperm samples) due to the toxicity of the cryoprotectant and the formation of ice crystals during freezing. In order to store precious sperm samples, at present, experts and scholars freeze a small amount of sperm samples by adopting micro sperm freezing carriers such as a zona pellucida, a cryloyop ring, a copper ring and an ICSI injection needle, but when the samples are frozen, the conventional micro sperm freezing carriers firstly use liquid nitrogen steam to fumigate the samples and then put the samples into the liquid nitrogen, so that the problems of low temperature reduction rate, complex operation and the like exist.
Disclosure of Invention
In order to solve the problems, the utility model aims to provide a carrier is preserved in freezing of trace sperm, easy operation not only can improve the refrigeration effect of sample moreover.
In order to achieve the above object, the utility model relates to a carrier is preserved in freezing of trace sperm, including the sperm straw, the sperm straw includes handle and thin slice, open the front end of thin slice has the through-hole that is used for forming the sample film.
As the preferred scheme, the thickness of thin slice is 0.3 ~ 0.8mm, the through-hole is total two, two the through-hole is arranged along the length direction of thin slice, and two through-holes are circular, and the diameter is 2 ~ 4 mm. The sample film is easily formed in the through-hole by optimizing the thickness of the sheet, and a spare sample film is provided by adding two through-holes.
As a preferred scheme, the micro sperm cryopreservation carrier further comprises a sleeve sleeved outside the sperm straws, one end of the sleeve is provided with an opening for the sperm straws to extend into, the other end of the sleeve is a closed end, and the closed end is provided with a hole for liquid nitrogen to flow into the sleeve. The sperm straws are protected by the sleeve pipe from being impacted by the outside so as to break the sample film, and in addition, the end part is provided with a hole so that the whole sleeve pipe is filled with liquid nitrogen.
More preferably, the sperm straw further comprises a connecting rod, the top end of the connecting rod is connected with the middle of the bottom surface of the handle, and the bottom end of the connecting rod is connected with the thin sheet. The handle and the slice are connected through the connecting rod, so that the product is more attractive and convenient to produce and process.
As a more preferable scheme, the diameter of the top end of the connecting rod is the same as the diameter of the inner wall of the sleeve, the diameter of the bottom of the handle is larger than the maximum diameter of the connecting rod, the top end face of the connecting rod is fixed in the middle of the bottom surface of the handle, the connecting rod is in a truncated cone shape with the diameter gradually decreasing from top to bottom, and the connecting rod and the bottom end of the handle form a T-shaped sealing plug structure for sealing the opening of the sleeve. The sperm straw is tightly connected with the sleeve by designing the connecting rod, and the connecting rod is used for sealing and fixing the straw and protecting a sample.
The invention has the advantages that: compare with current sperm cryopreservation carrier, the utility model discloses a trace sperm cryopreservation carrier opens at the front end of the thin slice of sperm straw and is used for forming the through-hole of sample film, scribbles the through-hole department of thin slice with trace sperm sample when using, forms one deck sample film on the through-hole promptly, and the sample film hangs in through-hole department, then arranges the sperm straw in the liquid nitrogen, and the upper and lower surface of sample film all contacts with the liquid nitrogen completely, realizes the rapid cooling of sample film, improves the freezing effect of sample.
Drawings
FIG. 1 is a schematic structural view of a micro sperm cryopreservation carrier according to the present invention;
FIG. 2 is an exploded view of FIG. 1;
the components in the figures are numbered as follows: sperm straw 10, handle 11, sheet 12, connecting rod 13, through hole 14, sleeve 20, opening 21, closed end 22.
Detailed Description
For a better understanding of the present invention, reference will now be made in detail to the present invention as illustrated in the accompanying drawings and specific examples.
Referring to fig. 1, the micro sperm cryopreservation carrier of this embodiment comprises a sperm straw 10 and a sleeve 20 sleeved outside the sperm straw 10.
The sleeve 20 is hollow tube, one end of the sleeve 20 is provided with an opening 21 for the sperm straw 10 to extend into, the other end is a closed end 22, and the middle part of the closed end 22 is provided with a hole for liquid nitrogen to flow into the sleeve 10, and the hole is a round hole with the diameter of 1 mm.
The sperm straw 10 comprises a handle 11, a sheet 12 and a connecting rod 13 for connecting the sheet 12 and the handle 11, wherein the front end of the sheet 12 is provided with two through holes 14 for forming a sample film, the thickness of the sheet is 0.5mm, the length of the sheet is 2.5mm, the width of the sheet is 5mm, the two through holes 14 are arranged along the length direction of the sheet 12, the two through holes are both circular, and the diameter of the two through holes is 3 mm; the connecting rod 13 is in a truncated cone shape, the diameter of the top end of the connecting rod 13 is larger than that of the bottom end of the connecting rod, the top end of the connecting rod 13 is connected with the handle 11, the bottom end of the connecting rod 13 is connected with the sheet 12, the diameter of the top end of the connecting rod 13 is the same as that of the inner wall of the sleeve 20, the diameter of the bottom surface of the handle 11 is larger than that of the top end of the connecting rod 13, the top end face of the connecting rod 13 is fixed in the middle of the bottom surface of the handle 11, as shown in fig. 2, the connecting rod 13 and the handle 11 form a sealing plug structure for sealing the opening end of the sleeve 20, in the embodiment, the diameter of the top end of the connecting rod 13 is 7 mm; wherein the top of connecting rod 13 is fixed at the middle part of handle 11 bottom surface, therefore connecting rod 13 and handle 11 constitute "T" shape sealing plug structure, have the opening 21 of sealed sleeve pipe 20 and the function of fixed sperm straw 10, fix sperm straw 10 on sleeve pipe 20 and prevent that sperm straw 10 from rocking at will in sleeve pipe 20 and damaging the sample film, design connecting rod 13 into the round platform shape in addition and be convenient for stretch into sleeve pipe 20 with thin slice 12 fast.
The application method of the micro sperm cryopreservation carrier comprises the steps of firstly placing a plurality of sleeves 20 in an aluminum bracket in a liquid nitrogen tank, and filling the sleeves 20 with liquid nitrogen through holes at the bottoms of the sleeves 20; after the sperm sample is prepared into a micro-droplet in the culture dish, under a microscope and with the help of a pipette or a pasteur tube, an operator holds a handle 11 of the sperm straw 10 by one hand, label paper marked with information such as sample source, freezing and related storage positions is pasted on the handle, the sperm sample in the culture dish is coated on two through holes 14 of a sheet 12 by the other hand, a layer of sample film is formed on each through hole 14 under the action of the liquid surface tension, each sample film has the liquid amount of about 3 microliter and is rapidly placed in liquid nitrogen, then the sample film extends into a sleeve 20 and is stored in a liquid nitrogen tank, and the whole operation process is completed in the liquid nitrogen.
During recovery, the 1006 dish is balanced and preheated in advance in the incubator, the preheated recovery liquid is made into a long strip shape, the surface of the recovery liquid is covered with mineral oil, the recovery liquid is balanced in the incubator for 30min, after the recovery liquid is prepared, the micro sperm cryopreservation carrier is taken out from the liquid nitrogen, the sleeve 20 is removed, the slice 12 is rapidly placed in the micromanipulation dish prepared in advance for unfreezing, the sample membrane falls into the micromanipulation dish after the unfreezing, and then the single sperm operation is carried out under a microscope.
The above-mentioned embodiments only represent one embodiment of the present invention, and the description thereof is specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (5)
1. The micro sperm cryopreservation carrier comprises a sperm straw (10), wherein the sperm straw (10) comprises a handle (11) and a thin sheet (12), and is characterized in that the front end of the thin sheet (12) is provided with a through hole (14) for forming a sample thin film.
2. The cryopreservation vehicle for micro sperm as claimed in claim 1, wherein the thickness of the sheet (12) is 0.3-0.8 mm, the number of the through holes (14) is two, the two through holes (14) are arranged along the length direction of the sheet (12), and the two through holes (14) are both circular and have a diameter of 2-4 mm.
3. The micro sperm cryopreservation carrier of claim 1 or 2, further comprising a sleeve (20) sleeved outside the sperm straw (10), wherein one end of the sleeve (20) is provided with an opening (21) for the sperm straw (10) to extend into, the other end is a closed end (22), and the closed end (22) is provided with a hole for nitrogen to flow into the sleeve.
4. The cryopreservation vehicle for micro sperm according to claim 3, wherein the sperm straw (10) further comprises a connecting rod (13), the top end of the connecting rod (13) is connected with the middle part of the bottom surface of the handle (11), and the bottom end of the connecting rod (13) is connected with the sheet (12).
5. The micro sperm cryopreservation carrier of claim 4, wherein the connecting rod (13) is in a truncated cone shape with a diameter gradually decreasing from top to bottom, the diameter of the top end of the connecting rod (13) is the same as that of the inner wall of the sleeve (20), the diameter of the bottom surface of the handle (11) is larger than the maximum diameter of the connecting rod (13), the top end surface of the connecting rod (13) is fixed in the middle of the bottom surface of the handle, and the bottom ends of the connecting rod (13) and the handle (11) form a T-shaped sealing plug structure for sealing the opening (21) of the sleeve (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020913835.7U CN212589916U (en) | 2020-05-26 | 2020-05-26 | Trace sperm cryopreservation carrier |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020913835.7U CN212589916U (en) | 2020-05-26 | 2020-05-26 | Trace sperm cryopreservation carrier |
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| CN212589916U true CN212589916U (en) | 2021-02-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202020913835.7U Active CN212589916U (en) | 2020-05-26 | 2020-05-26 | Trace sperm cryopreservation carrier |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113712025A (en) * | 2021-09-14 | 2021-11-30 | 荆州市中心医院 | Cryopreservation method of trace sperms and preparation method of sperms directly used for ICSI |
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2020
- 2020-05-26 CN CN202020913835.7U patent/CN212589916U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113712025A (en) * | 2021-09-14 | 2021-11-30 | 荆州市中心医院 | Cryopreservation method of trace sperms and preparation method of sperms directly used for ICSI |
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