CN203219847U

CN203219847U - Open type vitrification storage tube for bovine embryos and oocytes

Info

Publication number: CN203219847U
Application number: CN 201320250800
Authority: CN
Inventors: 徐惊涛; 孙永刚; 才让东智
Original assignee: Qinghai Academy of Animal Science and Veterinary Medicine
Current assignee: Qinghai Academy of Animal Science and Veterinary Medicine
Priority date: 2013-04-25
Filing date: 2013-04-25
Publication date: 2013-10-02
Anticipated expiration: 2023-04-25

Abstract

The utility model discloses an open type vitrification storage tube for bovine embryos and oocytes. The open type vitrification storage tube for bovine embryos and oocytes comprises an embryo refrigeration tube, wherein a sleeve which is matched with the refrigeration tube in a drawing-pulling manner is arranged on the outer surface of the refrigeration tube; a tube holding handle is fixed at one end of the embryo refrigeration tube; an embryo storage strip is fixed at the other end of the embryo refrigeration tube; a groove which is formed by once stamping is arranged in one side of the embryo refrigeration tube in a lateral mirrored manner; concave points are stamped at the two ends of the groove; a convex strip which is matched with the groove is laterally stamped on one side of the sleeve; convex points which are matched with the concave points are stamped at the two ends of the convex strip; a connecting strip is fixed on the sleeve; the other end of the connecting strip is fixedly connected with a sleeve cap; a cotton bolt is fixed on the top of the sleeve cap. The open type vitrification storage tube for bovine embryos and oocytes is simple in structure, delicate in conception, capable of effectively improving the vitrification success rate and efficiency, capable of simplifying the operation and convenient for storage.

Description

The open glass freezing of ox embryo and egg mother cell is preserved pipe

Technical field

The utility model belongs to the ox propagation technique to be promoted and application, and what be specifically related to is that the open glass freezing of ox embryo and egg mother cell is preserved pipe.

Background technology

Embryo biotechnology is to develop newer rapidly research field in the last few years, significant with application to livestock reproduction mechanism and breeding Research on New, the freezing preservation of livestock embryo and egg mother cell is exactly important techniques wherein, use this technology and be convenient to the storage and transport of embryo and egg mother cell, accelerate the improvement process that expansion is numerous and poultry is planted of domestic animal improved seeds, can promote embryo transfer technology application and popularization aborning, being one of key technology of embryo transfer technology industrialization, also is that the animal guarantor plants, set up the indispensable necessary means of gene pool; Superfreeze is preserved the two kinds of methods that mainly contain at present: the one, and the program freezing namely by embryo's sequencing frigorimeter, adopts the cryoprotector of low concentration, and little to the embryotoxicity infringement, the post-thaw survival rate is higher.But because of its complex operation, cooling rate is slow, causes required time long, needs the higher embryo cryopreservation instrument of cost, can not use this method to carry out embryo cryopreservation in the place that does not possess experiment condition.The 2nd, vitrifying freeze process; it is a newer freezing preservation technology; be that embryo or egg mother cell are put into the anti-frozen solution that contains high concentration; in temperature more than 0 ℃ directly drops into-196 ℃ liquid nitrogen; do not produce the ice crystal that destroys embryo or egg mother cell in the cooling procedure rapidly at this, thereby reach the purpose of long preservation.Compare with the program freezing, the advantage of vitrifying freeze process mainly is: egg mother cell or embryo directly contact with liquid nitrogen, and freezing rate obviously increases, easy and simple to handle, shortened the freezing required time greatly, do not needed expensive programmed cooling instrument, can operate in general laboratory.In Niu Shangyou research and application, have important theory and using value at aspects such as Livestock Production, local improved seeds and the conservations of wildlife at present.

The method that glass freezing is commonly used has: open elongation plastic straw OPS (open pulled straw) method, plastic straw method, electron microscopic speculum metal screen cloth (EM) vitrifying freeze process, liquid-transfering gun head (GL-tip), freezing cap (cryotop), freezing ring (cryoloop), little drop, nylon sieve method etc.At present the freezing and speed of thawing of OPS method is all greater than 20000 ℃/min; be 10 times of tubule method; and the time compole that embryo, egg mother cell contact with the high concentration freezeproof protectant is short; (more than 180 ℃ less than 30s); greatly reduced freezing injury; compare with other method that the OPS method is freezing, thaw routine is simplified greatly, can raise the efficiency.Therefore, design a kind of simple, conveniently, refrigerating effect is good, and the OPS method glass freezing apparatus of being convenient to preserve carries out embryo's freezing preservation, has important practical value.

The utility model content

The utility model purpose provides a kind of success rate and efficient that can improve glass freezing, simplifies the operation, and is convenient to preservation, provides a kind of simple, convenient, practical ox embryo and the open glass freezing preservation of egg mother cell to manage for studying and using.

In order to solve existing problem in the background technology, it comprises the embryo cryopreservation pipe, the outside of this cryovial is provided with the sleeve pipe that cooperates with its pull, described embryo cryopreservation Guan Yiduan fixedly holds the pipe handle, its other end fixedly has the embryo to store bar, one side side mirror of described embryo cryopreservation pipe looks like to be provided with the one time punching molded groove that goes out, the two ends punching press of this groove has concave point, one side side blow of described sleeve pipe is pressed with the raised line that matches with groove, the two ends punching press of this raised line has the salient point that matches with concave point, connection strap is fixedly arranged on the described sleeve pipe, and an other end of this connection strap is fixedlyed connected with cap bag, and tampon is fixedly arranged on the crown of described cap bag.

Described cap bag integral body is conical structure.

Owing to adopted above technical scheme, the utlity model has following beneficial effect: simple in structure, be skillfully constructed, can effectively improve success rate and the efficient of glass freezing, simplify the operation, be convenient to preserve.

Description of drawings

In order to be illustrated more clearly in the utility model, below in conjunction with accompanying drawing embodiment is done simple introduction.

Fig. 1 is structural representation of the present utility model;

Fig. 2 is the structural representation of cryovial in the utility model.

Embodiment

For technological means, creation characteristic that the utility model is realized, reach purpose and effect is easy to understand, the accompanying drawing below in conjunction with among the utility model embodiment is clearly and completely described the technical scheme among the utility model embodiment.

Referring to Fig. 1-2, this embodiment is to be achieved by the following technical solutions, it comprises embryo cryopreservation pipe 1, the outside of this cryovial 1 is provided with the sleeve pipe 2 that cooperates with its pull, described embryo cryopreservation pipe 1 one ends are fixedly held pipe handle 3, its other end fixedly has the embryo to store bar 4, one side side mirror of described embryo cryopreservation pipe 1 looks like to be provided with the one time punching molded groove 11 that goes out, the two ends punching press of this groove 11 has concave point 12, one side side blow of described sleeve pipe 2 is pressed with the raised line 21 that matches with groove 11, the two ends punching press of this raised line 21 has the salient point 22 that matches with concave point 12, on the described sleeve pipe 2 connection strap 5 is arranged fixedly, an other end of this connection strap 5 is fixedlyed connected with cap bag 6, and tampon 7 is fixedly arranged on the crown of described cap bag 6.

Described cap bag 6 integral body are conical structure.

Owing to adopted above technical scheme, this embodiment has following beneficial effect: simple in structure, be skillfully constructed, and can effectively improve success rate and the efficient of glass freezing, simplify the operation, be convenient to preserve.

During preservation; it is standby earlier embryo cryopreservation pipe 1 and the sleeve pipe 2 that is attached thereto to be carried out behind the soaking disinfection under ultraviolet lamp the illumination-based disinfection airing in alcohol; being prepared in advance before carrying out glass freezing will embryos frozen or egg mother cell; prepare corresponding glass freezing liquid; embryo or egg mother cell move into 2-3min in the freezing basal liquid; move into 3-4min in the freezing equilibrium liquid again; then embryo cryopreservation pipe 1 is taken out; holding pipe handle 3 draws sleeve pipe 2 outside buttons; making the embryo store bar 4 exposes; after observing the form of embryo and egg mother cell then; move in the freezing liquid; in microscopically 30s, transfer to embryo or egg mother cell on embryo's storage bar 4 of cryovial with the glass tubule; and in rapid the embryo's storage bar 4 and cap bag 6 immersion liquid nitrogen with front end; in liquid nitrogen, sleeve pipe 2 is outwards pushed; making the embryo store bar 4 is protected within the sleeve pipe 2; and then adopt tweezers in liquid nitrogen, to go into cap bag 6 lids to sleeve pipe 2 and hold out against, at last the embryo cryopreservation pipe 1 that is seated is put into all that liquid nitrogen container is medium-term and long-term to be preserved.

When thawing, earlier from liquid nitrogen, take out embryo cryopreservation pipe 1 in the heat-insulated container of being convenient to operate of putting into liquid nitrogen, with first the holding on the liquid level that pipe handle 3 is taken to liquid nitrogen and get with hand-held embryo cryopreservation pipe 1 of tweezers, its front end still is dipped in the liquid nitrogen, take off the cap bag 6 of front end then with tweezers, sleeve pipe 2 is drawn toward interior button, and embryo's storage bar 4 is exposed, rapidly the embryo cryopreservation pipe is shifted out from liquid nitrogen then, to embryo or egg mother cell be housed, storage bar 4 immerses and shifts to an earlier date in the ready 37 ℃ thawing solution, and the microscopically flushing is embryo or egg mother cell down, and embryo or egg mother cell are cultivated or transplant through namely can be used for after the cleaning again.

It should be noted that at last: above embodiment only in order to the technical solution of the utility model to be described, is not intended to limit; Although with reference to previous embodiment the utility model is had been described in detail, those of ordinary skill in the art is to be understood that, it still can be made amendment to the technical scheme that aforementioned each embodiment puts down in writing, and perhaps part technical characterictic wherein is equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution break away from the spirit and scope of each embodiment technical scheme of the utility model.

Claims

1. the open glass freezing of ox embryo and egg mother cell is preserved pipe, it is characterized in that it comprises the embryo cryopreservation pipe, the outside of this cryovial is provided with the sleeve pipe that cooperates with its pull, described embryo cryopreservation Guan Yiduan fixedly holds the pipe handle, its other end fixedly has the embryo to store bar, one side side mirror of described embryo cryopreservation pipe looks like to be provided with the one time punching molded groove that goes out, the two ends punching press of this groove has concave point, one side side blow of described sleeve pipe is pressed with the raised line that matches with groove, the two ends punching press of this raised line has the salient point that matches with concave point, connection strap is fixedly arranged on the described sleeve pipe, and an other end of this connection strap is fixedlyed connected with cap bag, and tampon is fixedly arranged on the crown of described cap bag.

2. the open glass freezing of ox embryo according to claim 1 and egg mother cell is preserved pipe, it is characterized in that described cap bag integral body is conical structure.