CN202588147U - Vitrified freezing carrier for embryos and oocytes of mammals - Google Patents
Vitrified freezing carrier for embryos and oocytes of mammals Download PDFInfo
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- CN202588147U CN202588147U CN 201220249115 CN201220249115U CN202588147U CN 202588147 U CN202588147 U CN 202588147U CN 201220249115 CN201220249115 CN 201220249115 CN 201220249115 U CN201220249115 U CN 201220249115U CN 202588147 U CN202588147 U CN 202588147U
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- metal weights
- tubule
- freezing tubule
- weights body
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Abstract
The utility model discloses a vitrified freezing carrier for embryos and oocytes of mammals. The vitrified freezing carrier comprises a thin freezing tube and a cone-shaped sucker which are matched and interlocked with each other, wherein a metal balance weight body is arranged at the rear end of the thin freezing tube; and a sealing interlayer is arranged between the metal balance weight body and an inner storage cavity of the thin freezing tube. The vitrified freezing carrier for the embryos and oocytes of the mammals disclosed by the utility model is low in cost and simple to operate, is made from common consumable materials, and can accurately control the amount of refrigerating liquid and freeze a plurality of oocytes or embryos for one time; by virtue of the metal balance weight body, specimens cannot float on the surface of liquid nitrogen and cannot be lost; the freezing speed and efficiency of the carrier are high, and the freezing injuries of the specimens are reduced; the carrier can be recycled, prevent the specimens from being broken or lost and save cost; and the thin tube can be marked, thus facitiating the long-time storage of the specimens.
Description
Technical field
The utility model relates to cell glass freezing technical field, particularly a kind of mammal embryo and egg mother cell vitrified frozen vector.
Background technology
Mammal ovocyte and embryo cryopreservation are the important component parts of human and animal's biology of reproduction.The particularly innovation and creation of glass freezing, can make freezing need be by the freezing instrument of routine, directly drop into liquid nitrogen after refrigeration material put into freezing liquid, simplified program greatly.The glass freezing technology is the freezing carrier that utilizes volume small; In the process of 2000 ℃ of coolings (> that is exceedingly fast/min); Make the frozen solution of high concentration become the solid-state of the very strong glass-like appearance of viscosity; Avoid the formation of ice crystal in the cell, reach the purpose of cryoprotection, thereby significantly improve cell, organize viability and developmental potency after freezing.
Vitrification method commonly used has: nylon ring vitrifying method (Cryoloop), open trombone slide method (OPS, Open Pulled Straw), fine glass tube method (GMP), micro drop method (Microdrops), copper mesh method, Cryoloop, Cryotop etc.
The selection of freezing carrier is extremely important in the glass freezing process, directly affects freezing efficiency.Reduce the freezing liquid of load when the function of freezing carrier is to carry sample as far as possible, reach and make sample directly contact liquid nitrogen in the refrigerating process, fast cooling reaches the vitrifying state, can remove the purpose of freezing liquid simultaneously in the course of defrosting again rapidly.Open trombone slide method is owing in light weightly be prone to float on the liquid nitrogen surface when freezing, and the tubule tube wall is thinner, in liquid nitrogen, very easily breaks at into sample and loses.Fine glass tube method easy fracture in refrigerating process is prone to cause sample to lose; Diameter and the volume of GMP are very little, have limited once freezing sample size, and this method siphon effect is remarkable, has increased operation easier.Micro drop method can't identify in storage process, is unfavorable for the long term storage of sample.Copper mesh method, Cryoloop and Cryotop exist sample to lose phenomenon in the freeze-thaw process.
The utility model content
To problem and defective that cell freezing in the present prior art exists, the utility model provides a kind of mammal embryo and egg mother cell vitrified frozen vector.
Technical scheme: a kind of mammal embryo and egg mother cell vitrified frozen vector; Comprise freezing tubule and taper suction nozzle; Both mate the make-up installation; The rear end of wherein said freezing tubule is equipped with the metal weights body, and depositing of this metal weights body and freezing tubule is provided with sealed compartment between the inner chamber.
The front end of said freezing tubule and taper suction nozzle junction are provided with at least one sheared edge longitudinally.
Said metal weights body is sealed in the rear end of freezing tubule fully, and perhaps, the freezing tubule sidewall of metal weights body contact is provided with the hole.
The front end of said metal weights body is provided with forked muscle, and said forked muscle passes to stretch into behind the interlayer and deposits in the inner chamber, and fits and be fixed on the madial wall of freezing tubule.
Said metal weights is external to be exposed to outside the freezing tubule; The metal weights body only has part to fix with freezing tubule suit; The front end of metal weights body is provided with forked muscle, and said forked muscle passes to stretch into behind the interlayer and deposits in the inner chamber, and fits and be fixed on the madial wall of freezing tubule.
The bore scope of the most advanced and sophisticated incision of said taper suction nozzle is 100 μ m～200 μ m.
Beneficial effect: 1. the utility model cost is low, and the consumptive material that manufacturing materials can be used always is simple to operate.2. can accurately control the amount of freezing liquid, and once can freezing many pieces of egg mother cells or embryo.3. adopt the metal weights body, sample can not float in the liquid nitrogen surface, can not lose sample.4. adopt the metal weights body to have heat conductivility preferably, make that chilling rate is fast, efficient is high, reduced the freezing injury of sample.Especially adopted to extend to the forked muscle of depositing in the inner chamber, thereby not only played the effect of supporting cryovial protection frozen cell, and be connected the effect with quickening cooling with the metal weights body, cooling-down effect is remarkable.5. carrier can reuse, and can not rupture and lose sample, practices thrift cost.On tubule, can make marks, be convenient to long preservation.
Description of drawings
Fig. 1 is one of freezing tubule cross-sectional view of the utility model vitrified frozen vector;
Fig. 2 be with Fig. 1 in freezing tubule cooperate the taper sucker structure sketch map of installing;
Fig. 3 be the utility model vitrified frozen vector freezing tubule cross-sectional view two;
Fig. 4 is the A portion structure for amplifying sketch map of Fig. 3;
Fig. 5 is the B-B cross-sectional view of Fig. 3;
Fig. 6 be the utility model vitrified frozen vector freezing tubule cross-sectional view three.
Embodiment
Embodiment one: referring to Fig. 1 and Fig. 2, this mammal embryo and egg mother cell vitrified frozen vector comprise freezing tubule 1 and taper suction nozzle 7, and both mate the make-up installation.
Among Fig. 1, the front end of freezing tubule 1 and taper suction nozzle 7 junctions are provided with at least one sheared edge 5 longitudinally, are convenient to and taper suction nozzle coupling suit, and have tightness.Among Fig. 2, the tip of taper suction nozzle 7 is provided with otch 71, and the other end is an installing port 72, and the bore scope at otch 71 places is 100 μ m～200 μ m.With Eppendorf 20 μ l pipettors taper suction nozzle 7 is installed, this carrier arrangement is together put into liquid nitrogen, suction is had the taper suction nozzle 7 of freezing sample insert freezing tubule 1 from sheared edge 5 with tweezers.After the freezing completion of whole samples, all freezing tubules 1 are put in the grape of liquid nitrogen container, put into liquid nitrogen container and can preserve for a long time.
The rear end of wherein said freezing tubule is equipped with metal weights body 2, and depositing of this metal weights body 2 and freezing tubule is provided with sealed compartment 3 between the inner chamber.Said metal weights body 2 is sealed in the rear end of freezing tubule 1 fully, and being convenient to freezing tubule can sink.In addition, metal weights body 2 can also quicken the effect that heat is transmitted, and improves freezing efficiency, has reduced the freezing injury of sample.
Embodiment two: referring to Fig. 3, Fig. 4 and Fig. 5, content and embodiment one are basic identical, and something in common does not repeat, and different is: the freezing tubule sidewall of metal weights body 2 contacts is provided with hole 6.And; The front end of said metal weights body 2 is provided with forked muscle 21; Said forked muscle 21 passes to stretch into behind the interlayer and deposits in the inner chamber 11, and fits and be fixed on the madial wall of freezing tubule 1, and forked muscle 21 not only has and strengthens and the support effect supporting freezing tubule; And be connected with the metal weights body and have the effect of accelerating cooling, cooling-down effect is remarkable.
Embodiment three: referring to Fig. 6; Content and embodiment two are basic identical, and something in common does not repeat, and different is: said metal weights body 2 exposes to outside the freezing tubule; Metal weights body 2 only has part to fix with freezing tubule 1 suit; The front end of metal weights body 2 is provided with forked muscle 21, and said forked muscle 21 passes to stretch into behind the interlayer 3 and deposits in the inner chamber 11, and fits and be fixed on the madial wall of freezing tubule 1.The metal weights body 2 that leaks outside is more conducive to conductive force.
Claims (6)
1. mammal embryo and egg mother cell vitrified frozen vector; It is characterized in that: comprise freezing tubule and taper suction nozzle; Both mate the make-up installation; The rear end of wherein said freezing tubule is equipped with the metal weights body, and depositing of this metal weights body and freezing tubule is provided with sealed compartment between the inner chamber.
2. mammal embryo according to claim 1 and egg mother cell vitrified frozen vector is characterized in that: the front end of said freezing tubule and taper suction nozzle junction are provided with at least one sheared edge longitudinally.
3. mammal embryo according to claim 1 and egg mother cell vitrified frozen vector is characterized in that: said metal weights body is sealed in the rear end of freezing tubule fully, and perhaps, the freezing tubule sidewall of metal weights body contact is provided with the hole.
4. mammal embryo according to claim 1 and egg mother cell vitrified frozen vector; It is characterized in that: the front end of said metal weights body is provided with forked muscle; Said forked muscle passes to stretch into behind the interlayer and deposits in the inner chamber, and fits and be fixed on the madial wall of freezing tubule.
5. mammal embryo according to claim 1 and egg mother cell vitrified frozen vector; It is characterized in that: said metal weights is external to be exposed to outside the freezing tubule; The metal weights body only has part to fix with freezing tubule suit; The front end of metal weights body is provided with forked muscle, and said forked muscle passes to stretch into behind the interlayer and deposits in the inner chamber, and fits and be fixed on the madial wall of freezing tubule.
6. according to each described mammal embryo of claim 1-5 and egg mother cell vitrified frozen vector, it is characterized in that: the bore scope of the most advanced and sophisticated incision of said taper suction nozzle is 100 μ m～200 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220249115 CN202588147U (en) | 2012-05-30 | 2012-05-30 | Vitrified freezing carrier for embryos and oocytes of mammals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220249115 CN202588147U (en) | 2012-05-30 | 2012-05-30 | Vitrified freezing carrier for embryos and oocytes of mammals |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202588147U true CN202588147U (en) | 2012-12-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220249115 Expired - Fee Related CN202588147U (en) | 2012-05-30 | 2012-05-30 | Vitrified freezing carrier for embryos and oocytes of mammals |
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| Country | Link |
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| CN (1) | CN202588147U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494316A (en) * | 2016-01-22 | 2016-04-20 | 四川大学华西第二医院 | Human division stage embryo and blastula closed frozen carrier and low-temperature preservation method |
-
2012
- 2012-05-30 CN CN 201220249115 patent/CN202588147U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494316A (en) * | 2016-01-22 | 2016-04-20 | 四川大学华西第二医院 | Human division stage embryo and blastula closed frozen carrier and low-temperature preservation method |
| CN105494316B (en) * | 2016-01-22 | 2018-09-07 | 四川大学华西第二医院 | People's division stage embryo and the closed freezing carrier of blastaea and Cryopreservation |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121212 Termination date: 20130530 |