CN202603479U - Vitrified freezing device for embryos and oocytes of mammal - Google Patents
Vitrified freezing device for embryos and oocytes of mammal Download PDFInfo
- Publication number
- CN202603479U CN202603479U CN 201220278780 CN201220278780U CN202603479U CN 202603479 U CN202603479 U CN 202603479U CN 201220278780 CN201220278780 CN 201220278780 CN 201220278780 U CN201220278780 U CN 201220278780U CN 202603479 U CN202603479 U CN 202603479U
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- freezing
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- sample
- freezing device
- vitrified
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Abstract
The utility model discloses a vitrified freezing device for embryos and oocytes of mammal. The vitrified freezing device comprises a freezing carrier and a latex tubing; the freezing carrier is of a laminated structure, wherein one end of the freezing carrier is wider than the other end of the freezing carrier, a marking area is arranged at the narrower end, and a frozen cell sample is loaded in the marking area; and the narrower end of the laminated freezing carrier can be inserted into the latex tubing. The verified freezing device is low in cost because manufacturing materials are common consumable, simple to operate, large in freezing area because the width can reach 1cm and multiple oocytes and embryos can be frozen once, incapable of losing the sample because the sample cannot float on the liquid nitrogen surface, rapid in freezing speed, high in efficiency and reduced in freezing damage of the sample. The freezing efficiency of the vitrified freezing device has no remarkable difference compared with that of other methods; and the packaged carrier is subjected to gas sterilization and can be recycled, and the sample cannot be broken or lose, therefore the cost is saved. The latex tubing can be marked and is convenient to store for a long time.
Description
Technical field
The utility model relates to cell glass freezing technical field, particularly a kind of mammal embryo and egg mother cell glass freezing device.
Background technology
Mammal ovocyte and embryo cryopreservation are the important component parts of human and animal's biology of reproduction.The particularly invention of glass freezing, can make freezing need be by the freezing instrument of routine, directly drop into liquid nitrogen after refrigeration material put into freezing liquid, simplified program greatly.The glass freezing technology is the freezing carrier that utilizes volume small; In the process of 2000 ℃ of coolings (> that is exceedingly fast/min); Make the frozen solution of high concentration become the solid-state of the very strong glass-like appearance of viscosity; Avoid the formation of ice crystal in the cell, reach the purpose of cryoprotection, thereby significantly improve cell, organize viability and developmental potency after freezing.
Vitrification method commonly used has: nylon ring vitrifying method (Cryoloop), open trombone slide method (OPS, Open Pulled Straw), fine glass tube method (GMP), micro drop method (Microdrops), copper mesh method, Cryoloop, Cryotop etc.
The selection of freezing carrier is extremely important in the glass freezing process, directly affects freezing efficiency.Reduce the freezing liquid of load when the function of freezing carrier is to carry sample as far as possible, reach and make sample directly contact liquid nitrogen in the refrigerating process, fast cooling reaches the vitrifying state, can remove the purpose of freezing liquid simultaneously in the course of defrosting again rapidly.Open trombone slide method is owing in light weightly be prone to float on the liquid nitrogen surface when freezing, and the tubule tube wall is thinner, in liquid nitrogen, very easily breaks at into sample and loses.Fine glass tube method easy fracture in refrigerating process is prone to cause sample to lose; Diameter and the volume of GMP are very little, have limited once freezing sample size, and this method siphon effect is remarkable, has increased operation easier.Micro drop method can't identify in storage process, is unfavorable for the long term storage of sample.Copper mesh method, Cryoloop exist sample to lose phenomenon in the freeze-thaw process.The existing ripe commodity listing of Cryotop method; The shortcoming of this method is that to deposit embryo's Cryotop sheet very narrow, and about 1mm is very skilled in the time of operation; Can only freeze one piece of egg mother cell or embryo because of receiving condition to limit each only to install, and cost an arm and a leg.
The utility model content
To problem and defective that cell freezing in the present prior art exists, the utility model provides a kind of mammal embryo and egg mother cell glass freezing device.
Technical scheme: a kind of mammal embryo and egg mother cell glass freezing device; Comprise freezing carrier and emulsion tube, this freezing carrier is a laminated structure, and one of which end width is greater than other end width; End narrower is provided with marked region, loads the frozen cell sample in the marked region; The narrow end of the freezing carrier of sheet can insert in the emulsion tube.
The freezing carrier of said sheet is an isosceles trapezoidal structure, and the width of an end of its broad is 1.1 ~ 1.4cm, and the width of the end that it is narrower is 0.9 ~ 1.2cm, the about 4 ~ 5cm of the height of isosceles trapezoidal structure, and marked region is 0.9 ~ 1.2cm apart from the length of narrow end.
Diameter 0.8 ~ the 1.1cm of said emulsion tube, length 3.5 ~ 4.5cm.
Be provided with adhesive tape or label that different colours is used for mark at the middle part of emulsion tube.
Beneficial effect: 1. the utility model cost is low, and manufacturing materials is consumptive material commonly used, and is simple to operate.2. freezing area is big, and width reaches 1cm, once can freezing many pieces of egg mother cells or embryo, and Crytop once can only freezing one piece of sample.3. sample can not float in the liquid nitrogen surface, can not lose sample.4. speed of cooling is fast, efficient is high, has reduced the freezing injury of sample.Freezing efficiency and additive method difference are not remarkable.5. gaseous sterilization can reuse behind the carrier package, can not rupture and lose sample, practices thrift cost.On emulsion tube, can make marks, be convenient to long preservation.
Description of drawings
Fig. 1 is the utility model emulsion tube structural representation;
Fig. 2 cooperates the sheet freezing carrier structural representation of installing with Fig. 1 emulsion tube;
Fig. 3 is the cross-sectional view after Fig. 1 and Fig. 2 assembling.
Embodiment
Embodiment one: referring to Fig. 1 and Fig. 2, this mammal embryo and egg mother cell glass freezing device comprise the freezing carrier 2 and emulsion tube 1 of sheet.Wherein an end width of sheet freezing carrier 2 is greater than the isosceles trapezoidal structure of other end width, and the width of thicker end 22 is 1.2cm, and the width of narrow end 21 is 1cm, the about 4 ~ 5cm of the height of isosceles trapezoidal structure.End narrower is provided with marked region 4, and marked region 4 is 1cm apart from the length of narrow end.Load the frozen cell sample in the marked region; The narrow end of the freezing carrier of sheet can insert in the emulsion tube.Diameter 0.9 ~ the 1cm of said emulsion tube, length is about 4cm.Be provided with the adhesive tape or the label 5 of different colours at the middle part of emulsion tube.
Claims (4)
1. mammal embryo and egg mother cell glass freezing device; It is characterized in that: comprise freezing carrier and emulsion tube, this freezing carrier is a laminated structure, and one of which end width is greater than other end width; End narrower is provided with marked region, loads the frozen cell sample in the marked region; The narrow end of the freezing carrier of sheet can insert in the emulsion tube.
2. mammal embryo according to claim 1 and egg mother cell glass freezing device; It is characterized in that: the freezing carrier of said sheet is an isosceles trapezoidal structure; The width of one end of its broad is 1.1 ~ 1.4cm; The width of the end that it is narrower is 0.9 ~ 1.2cm, the about 4 ~ 5cm of the height of isosceles trapezoidal structure, and marked region is 0.9 ~ 1.2cm apart from the length of narrow end.
3. mammal embryo according to claim 1 and egg mother cell glass freezing device is characterized in that: the diameter 0.8 ~ 1.1cm of said emulsion tube, length 3.5 ~ 4.5cm.
4. mammal embryo according to claim 1 and egg mother cell glass freezing device is characterized in that: be provided with adhesive tape or the label that is used for mark at the middle part of emulsion tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220278780 CN202603479U (en) | 2012-06-14 | 2012-06-14 | Vitrified freezing device for embryos and oocytes of mammal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220278780 CN202603479U (en) | 2012-06-14 | 2012-06-14 | Vitrified freezing device for embryos and oocytes of mammal |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202603479U true CN202603479U (en) | 2012-12-19 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220278780 Expired - Fee Related CN202603479U (en) | 2012-06-14 | 2012-06-14 | Vitrified freezing device for embryos and oocytes of mammal |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202603479U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108293980A (en) * | 2018-03-07 | 2018-07-20 | 山东国源人类遗传资源库管理有限公司 | A kind of neural molecular biology glass frozen preservation/method for resuscitation |
| CN110214776A (en) * | 2019-07-01 | 2019-09-10 | 安徽农业大学 | It is a kind of based on straw freezing Tip carrier pig blastocyst freeze and defreezing method |
-
2012
- 2012-06-14 CN CN 201220278780 patent/CN202603479U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108293980A (en) * | 2018-03-07 | 2018-07-20 | 山东国源人类遗传资源库管理有限公司 | A kind of neural molecular biology glass frozen preservation/method for resuscitation |
| CN108293980B (en) * | 2018-03-07 | 2020-10-30 | 国源生命科学集团有限公司 | Vitrification cryopreservation/recovery method for neural stem cell spheres |
| CN110214776A (en) * | 2019-07-01 | 2019-09-10 | 安徽农业大学 | It is a kind of based on straw freezing Tip carrier pig blastocyst freeze and defreezing method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121219 Termination date: 20140614 |
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| EXPY | Termination of patent right or utility model |