CN204796589U - Red blood cell liquid nitrogen drips form save set - Google Patents
Red blood cell liquid nitrogen drips form save set Download PDFInfo
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- CN204796589U CN204796589U CN201520094802.3U CN201520094802U CN204796589U CN 204796589 U CN204796589 U CN 204796589U CN 201520094802 U CN201520094802 U CN 201520094802U CN 204796589 U CN204796589 U CN 204796589U
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- liquid nitrogen
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- blood cell
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Abstract
The patent of the utility model relates to a device belongs to medical appliances technical field that is used for a red blood cell liquid nitrogen form to preserve . the utility model discloses an outer box, interior box and grid. Outer box is stainless steel for the design of stainless steel dewar bottle, interior box and grid. The utility model discloses box in the characterized in that grid can be arranged in, outer box can be arranged in to interior box, interior box bottom portion is the stainless steel screen cloth. The utility model discloses a little check of a plurality of squares are cut apart into with interior box to the grid, can instil into in the different little check (1 / check) with red cytomixis thing in operation process to prevent that red cell freezing tear drop is agglutinate each other in the liquid nitrogen, simultaneously, arrange to outer box through can pass through the stainless steel screen cloth with liquid nitrogen in the interior box fast from box in mentioning in the outer box, and red cell freezing tear drop since the diameter is great can't stay through the stainless steel screen cloth in the box, the collection of the red cell freezing tear drop of being convenient for to save operating time, improve work efficiency.
Description
Technical field
The utility model relates to a kind of device preserved for red blood cell liquid nitrogen drop-wise, belongs to technical field of medical instrument.
Background technology
The various reagent red blood cell of modern blood group serology need of work, comprises the red blood cell of screening cell, spectrum cell and various special or rare blood type.In blood group antigen research and Identification of the antibodies, these red blood cells are very important reagent, and some cell is very rare in crowd, compare and are difficult to obtain.And in routine duties, likely finding some valuable or rare red blood cells, after discovery, permanently effective preservation immediately just seems particularly important.
It is Liquid nitrogen storage that red blood cell preserves optimal mode for a long time.The temperature of liquid nitrogen is-196 ° of C, at this temperature erythrocyte metabolism be tending towards stagnate, and recovery after erythrocytic structure and immunologic function intact.Red cell preservation mode conventional is at present red blood cell liquid nitrogen drop-wise Techniques of preserving.In red blood cell liquid nitrogen drop-wise is preserved, red blood cell is preserved with the form of spherical bead trace, and every pearl, can at any time according to blood group serology requirements of one's work about containing 25 to 50 microlitre red blood cell homomixtures, take out one or more globule and melt recovery, facilitate normal experiment work to use.First by centrifugal for anticoagulation cirumferential blood removing blood plasma during concrete operations, use brine red blood cell 1 time, the conserving liquid of the packed red cells of 1 volume and 1 volume is mixed, equilibrium at room temperature 10 minutes, bring protective gloves, get certain volume container and load appropriate liquid nitrogen, draw above-mentioned red blood cell homomixture with pasteur pipet and drop by drop instill in liquid nitrogen.Red blood cell homomixture quick freeze in liquid nitrogen becomes red globules (the freezing tear drop of red blood cell), then to be collected in by freezing for red blood cell tear drop in cryopreservation tube and to be placed in the medium-term and long-term preservation of liquid nitrogen container.
The freezing tear drop of red blood cell is formed because red blood cell homomixture needs certain hour (about 10 seconds) to cool after instillation liquid nitrogen, at the freezing tear drop of red blood cell will do not cooled completely in red blood cell homomixture instillation liquid nitrogen process, easily aggegation is agglomerating mutually, therefore must carry out preventing the mutual aggegation of the freezing tear drop of red blood cell agglomerating with every 10 seconds speed of 1 by during red blood cell homomixture instillation liquid nitrogen, cause the whole frozen process operation time longer like this, and consume more liquid nitrogen.In addition, the cryopreservation tube that freezing for red blood cell tear drop is collected into mark is good need be preserved by operator for a long time, due to red blood cell freezing tear drop volume gently little, in spherical and fly in liquid nitrogen, the operator with protective gloves is difficult to from liquid nitrogen, takes out the freezing tear drop of red blood cell and put into cryopreservation tube.
Summary of the invention
In the market temporarily without the relevant product being specifically designed to red blood cell liquid nitrogen drop-wise and preserving.The utility model proposes a kind of device preserved for red blood cell liquid nitrogen drop-wise, solve the freezing tear drop of the red blood cell problem that easily aggegation is agglomerating and the collection of red blood cell freezing tear drop is difficult mutually in liquid nitrogen, save the operating time, decrease the liquid nitrogen consumption in operating process, increase work efficiency, be adapted at promoting the use of in association area.
The utility model comprises outer box, inner box and grid; Outer box adopts stainless steel dewars designs, and inner box and grid adopt stainless steel.
Described outer box and inner box are the rectangular structure of hollow, uncovered, grid is little lattice design (length of side is 1 centimetre) of 4 × 6=24 square, the length, width and height of grid are all less than the length, width and height of inner box, the length and width of inner box are all less than the length and width of outer box, grid can be placed in inner box easily, thus inner box being separated into some little lattice, the described inner box containing grid can be placed in outer box easily.
Described inner box both sides have a pair inner box handle, and inner box can take out easily from outer box; Described grid both sides have a pair grid handle, and grid can take out easily from inner box.
Be 18 order stainless steel mesh bottom described inner box, when being mentioned from outer box by inner box, in inner box, liquid nitrogen drains into outer box by 18 order stainless steel mesh, and the freezing tear drop of red blood cell cannot be stayed in inner box by 18 order stainless steel mesh more greatly due to diameter.
The utility model mainly has following beneficial effect:
1, the utility model proposes a kind of device preserved for red blood cell liquid nitrogen drop-wise, by putting grid into inner box thus inner box being separated into some little lattice, first in inner box, appropriate liquid nitrogen is injected during operation, by red blood cell homomixture instillation liquid nitrogen time without the need to carrying out with the every 10 seconds speed of 1, instilled in different little lattice by red blood cell homomixture serially, each aperture drips, and easily aggegation is agglomerating mutually can to prevent the freezing tear drop of red blood cell, can save time again, and raise the efficiency;
2, the utility model proposes a kind of device preserved for red blood cell liquid nitrogen drop-wise, the inner box design of 18 order stainless steel mesh is provided with by outer box and bottom, can after red blood cell homomixture instillation liquid nitrogen end of operation, first grid is proposed from inner box, again inner box is mentioned from outer box, liquid nitrogen 18 order stainless steel mesh bottom inner box flow to outer box, and the freezing tear drop of red blood cell cannot be stayed in inner box by 18 order stainless steel mesh more greatly due to diameter, thus realize being separated of the freezing tear drop of red blood cell and liquid nitrogen, handled easily person carries out the collection work of the freezing tear drop of red blood cell.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail:
Fig. 1 is the structural representation that the utility model proposes.
As shown in the figure, in figure, various piece is represented by following Arabic numerals:
1, outer box, 2, inner box, 3, grid, 4,18 order stainless steel mesh, 5, inner box handle, 6, grid handle.
Embodiment
The utility model proposes a kind of device preserved for red blood cell liquid nitrogen drop-wise, as shown in Figure 1, comprise outer box 1, inner box 2, grid 3,18 order stainless steel mesh 4, inner box handle 5 and grid handle 6.
Described outer box is the rectangular structure of hollow, and as shown in Figure 1, opening upwards, without lid.
Described inner box is the rectangular structure of hollow, is placed in outer box, and as shown in the figure, the length and width of inner box are all less than the length and width of outer box, conveniently inner box is placed in outer box.
The bottom of described inner box is 18 order stainless steel mesh, and handled easily person carries out the collection work of the freezing tear drop of red blood cell.
Described grid is the little lattice design of 4 × 6=24, and the length, width and height of grid are all less than the length, width and height of inner box, are conveniently placed in inner box by grid.The both sides of grid have a pair handle, are conveniently proposed from inner box by grid.
Apply red blood cell liquid nitrogen drop-wise save set provided by the utility model carry out red blood cell frozen time, first experimenter first will prepare alserver's solution, again by centrifugal for anticoagulation cirumferential blood removing blood plasma, use brine red blood cell 1 time, the conserving liquid of 1 volume packed red cells and 1 volume is mixed, equilibrium at room temperature 10 minutes.Operating personnel should bring protective gloves, and grid is placed in inner box, then the inner box that grid is housed is placed in outer box.Appropriate liquid nitrogen is slowly injected in inner box, liquid nitrogen slowly penetrates in outer box by 18 order stainless steel mesh bottom inner box, when the liquid nitrogen liquid level height of inner box and outer box is equal, draw in red blood cell homomixture instillation grid aperture with pasteur pipet, each aperture drips, red blood cell homomixture drop is the freezing tear drop of quick freeze erythroblast in liquid nitrogen, to intercept and red blood cell homomixture is only dripped in every hole because grid aperture surrounding has, effectively can avoid the mutual conglomerate of the freezing tear drop of red blood cell.After red blood cell homomixture all drips off, first grid is slowly mentioned from inner box, again inner box is slowly mentioned from outer box, liquid nitrogen now in inner box slowly flows in outer box by bottom 18 order stainless steel mesh, and the freezing tear drop of red blood cell cannot be stayed in inner box by 18 order stainless steel mesh more greatly due to diameter, after in inner box, liquid nitrogen drains, freezing for red blood cell in inner box tear drop is collected in the good cryopreservation tube of mark, and be transferred in liquid nitrogen container and preserve, thus complete red blood cell liquid nitrogen drop-wise and preserve operation.
In a word; those skilled in the art although the utility model lists above-mentioned preferred embodiment, should illustrate, although can carry out various change and remodeling; unless such change and remodeling deviate from scope of the present invention, otherwise all should be included in protection domain of the present utility model.
Claims (7)
1. red blood cell liquid nitrogen drop-wise save set, comprise outer box (1), inner box (2), grid (3), it is characterized in that: described outer box (1) and inner box (2) are the rectangular structure of hollow, uncovered, the length and width of inner box (2) are all less than the length and width of outer box (1), and the bottom of inner box (2) is 18 order stainless steel mesh (4); Described grid (3) is the grid design that 4 row 6 arrange, and the length, width and height of grid (3) are all less than the length, width and height of inner box (2).
2. red blood cell liquid nitrogen drop-wise save set according to claim 1, is characterized in that: described outer box (1) adopts stainless steel dewars designs.
3. red blood cell liquid nitrogen drop-wise save set according to claim 1, is characterized in that: described inner box (2) and grid (3) all adopt stainless steel.
4. red blood cell liquid nitrogen drop-wise save set according to claim 3, is characterized in that: described grid (3) can be placed in inner box (2) easily, thus inner box is separated into some little lattice; The described inner box (2) containing grid (3) can be placed in outer box (1) easily.
5. red blood cell liquid nitrogen drop-wise save set according to claim 1, is characterized in that: be 18 order stainless steel mesh (4) bottom described inner box.
6. red blood cell liquid nitrogen drop-wise save set according to claim 1, is characterized in that: described grid (3) both sides have a pair grid handle (6).
7. red blood cell liquid nitrogen drop-wise save set according to claim 1, is characterized in that: described inner box (2) both sides have a pair inner box handle (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520094802.3U CN204796589U (en) | 2015-02-11 | 2015-02-11 | Red blood cell liquid nitrogen drips form save set |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520094802.3U CN204796589U (en) | 2015-02-11 | 2015-02-11 | Red blood cell liquid nitrogen drips form save set |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204796589U true CN204796589U (en) | 2015-11-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520094802.3U Expired - Fee Related CN204796589U (en) | 2015-02-11 | 2015-02-11 | Red blood cell liquid nitrogen drips form save set |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204796589U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107751184A (en) * | 2017-10-18 | 2018-03-06 | 南方医科大学珠江医院 | A kind of glass frozen preservation box of operation readiness |
| CN108651440A (en) * | 2018-04-18 | 2018-10-16 | 中南大学 | A kind of supper-fast freezen protective system of human seminal fluid |
-
2015
- 2015-02-11 CN CN201520094802.3U patent/CN204796589U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107751184A (en) * | 2017-10-18 | 2018-03-06 | 南方医科大学珠江医院 | A kind of glass frozen preservation box of operation readiness |
| CN108651440A (en) * | 2018-04-18 | 2018-10-16 | 中南大学 | A kind of supper-fast freezen protective system of human seminal fluid |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151125 Termination date: 20160211 |