CN102669088B - Mammal embryo and oocyte vitrification freezing carrier - Google Patents
Mammal embryo and oocyte vitrification freezing carrier Download PDFInfo
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- CN102669088B CN102669088B CN 201210172951 CN201210172951A CN102669088B CN 102669088 B CN102669088 B CN 102669088B CN 201210172951 CN201210172951 CN 201210172951 CN 201210172951 A CN201210172951 A CN 201210172951A CN 102669088 B CN102669088 B CN 102669088B
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Abstract
The invention discloses a mammal embryo and oocyte vitrification freezing carrier. The mammal embryo and oocyte vitrification freezing carrier comprises a freezing tubule and a tapered sucking head both of which are mounted in a matching and butting manner, wherein a metal weight body is arranged at the back end of the freezing tubule; and a sealing interlayer is arranged between the metal weight body and an inner storage cavity of the freezing tubule. The mammal embryo and oocyte vitrification freezing carrier is low in cost as the manufacturing materials are the common materials, is easy to operate and is capable of controlling amount of a freezing liquid precisely and freezing multiple oocytes and embryos for once. As the metal weight body is adopted, a sample cannot float on a liquid nitrogen surface and the sample cannot be lost; freezing damage of the sample is reduced due to the fast freezing speed and high freezing efficiency; and the carrier can be utilized repeatedly and cannot break to cause lost of the sample, so that cost is saved. Marks can be made on the tubule, so that convenience can be brought about for long-term preservation.
Description
Technical field
The present invention relates to cell glass freezing technical field, particularly a kind of mammal embryo and egg mother cell vitrified frozen vector.
Background technology
Mammal ovocyte and embryo cryopreservation are the important component parts of human and animal's biology of reproduction.The particularly invention of glass freezing, can make freezing need be by the freezing instrument of routine, directly drop into liquid nitrogen after refrigeration material put into freezing liquid, simplified program greatly.The glass freezing technology is the freezing carrier that utilizes volume small; in the process of 2000 ℃ of coolings (〉 that is exceedingly fast/min); make the frozen solution of high concentration become very strong similar hyaloid solid-state of viscosity; avoid the formation of ice crystal in the cell; reach the purpose of cryoprotection, thereby significantly improve cell, organize viability and developmental potency after freezing.
Vitrification method commonly used has: nylon ring vitrifying method (Cryoloop), open trombone slide method (OPS, Open Pulled Straw), fine glass tube method (GMP), micro drop method (Microdrops), copper mesh method, Cryoloop, Cryotop etc.
The selection of freezing carrier is extremely important in the glass freezing process, directly affects freezing efficiency.Reduce the freezing liquid of load when the function of freezing carrier is to carry sample as far as possible, reach and make sample directly contact liquid nitrogen in the refrigerating process, fast cooling reaches the vitrifying state, can remove the purpose of freezing liquid simultaneously in the course of defrosting again rapidly.Open trombone slide method is owing in light weightly easily float on the liquid nitrogen surface when freezing, and the tubule tube wall is thinner, very easily breaks at into sample and lose in liquid nitrogen.Fine glass tube method easy fracture in refrigerating process easily causes sample to lose; Diameter and the volume of GMP are very little, limited once freezing sample size, and this method siphon effect are remarkable, increased operation easier.Micro drop method can't identify in storage process, is unfavorable for the long term storage of sample.Copper mesh method, Cryoloop and Cryotop exist sample to lose phenomenon in the freeze-thaw process.
Summary of the invention
Problem and defective at cell freezing in the present prior art exists the invention provides a kind of mammal embryo and egg mother cell vitrified frozen vector.
Technical scheme: a kind of mammal embryo and egg mother cell vitrified frozen vector, comprise freezing tubule and taper suction nozzle, both mate the make-up installation, the rear end of wherein said freezing tubule is equipped with the metal weights body, and depositing of this metal weights body and freezing tubule is provided with sealed compartment between the inner chamber.
The front end of described freezing tubule and taper suction nozzle junction are provided with at least one sheared edge longitudinally.
Described metal weights body is sealed in the rear end of freezing tubule fully, and perhaps, the freezing tubule sidewall of metal weights body contact is provided with the hole.
The front end of described metal weights body is provided with forked muscle, and described forked muscle passes to stretch into behind the interlayer and deposits in the inner chamber, and fits and be fixed on the madial wall of freezing tubule.
Described metal weights is external to be exposed to outside the freezing tubule, the metal weights body only has part to fix with freezing tubule suit, the front end of metal weights body is provided with forked muscle, and described forked muscle passes to stretch into behind the interlayer and deposits in the inner chamber, and fits and be fixed on the madial wall of freezing tubule.
The bore scope of the most advanced and sophisticated incision of described taper suction nozzle is 100 μ m～200 μ m.
Beneficial effect: 1. cost of the present invention is low, makes the consumptive material that material can be used always, and is simple to operate.2. can accurately control the amount of freezing liquid, and once can freezing many pieces of egg mother cells or embryo.3. adopt the metal weights body, sample can not float in the liquid nitrogen surface, can not lose sample.4. adopt the metal weights body to have heat conductivility preferably, make that chilling rate is fast, efficient is high, reduced the freezing injury of sample.Especially adopted to extend to the forked muscle of depositing in the inner chamber, thereby not only played the effect of supporting cryovial protection frozen cell, and be connected the effect with quickening cooling with the metal weights body, cooling-down effect is remarkable.5. carrier can reuse, and can not rupture and lose sample, saves cost.On tubule, can make marks, be convenient to long preservation.
Description of drawings
Fig. 1 is one of freezing tubule cross-sectional view of vitrified frozen vector of the present invention;
Fig. 2 cooperates the taper sucker structure schematic diagram of installing with freezing tubule among Fig. 1;
Fig. 3 be vitrified frozen vector of the present invention freezing tubule cross-sectional view two;
Fig. 4 is the A portion structure for amplifying schematic diagram of Fig. 3;
Fig. 5 is the B-B cross-sectional view of Fig. 3;
Fig. 6 be vitrified frozen vector of the present invention freezing tubule cross-sectional view three.
Number in the figure 1 is freezing tubule, 11 inner chambers of depositing for freezing tubule, and 2 is the metal weights body, and 21 is forked muscle, and 3 is interlayer, and 4 for sealing, and 5 is sheared edge, and 6 is the hole, and 7 are the taper suction nozzle, and 71 is otch, and 72 is installing port.
Embodiment
Embodiment one: referring to Fig. 1 and Fig. 2, this mammal embryo and egg mother cell vitrified frozen vector comprise freezing tubule 1 and taper suction nozzle 7, and both mate the make-up installation.
Among Fig. 1, the front end of freezing tubule 1 and taper suction nozzle 7 junctions are provided with at least one sheared edge 5 longitudinally, are convenient to and taper suction nozzle coupling suit, and have tightness.Among Fig. 2, the tip of taper suction nozzle 7 arranges otch 71, and the other end is installing port 72, and the bore scope at otch 71 places is 100 μ m～200 μ m.With Eppendorf 20 μ l pipettors taper suction nozzle 7 is installed, this carrier arrangement is together put into liquid nitrogen, suction is had the taper suction nozzle 7 of freezing sample insert freezing tubule 1 from sheared edge 5 with tweezers.After finishing etc. whole samples are freezing, all freezing tubules 1 are put in the grape of liquid nitrogen container, put into liquid nitrogen container and can preserve for a long time.
The rear end of wherein said freezing tubule is equipped with metal weights body 2, and depositing of this metal weights body 2 and freezing tubule is provided with sealed compartment 3 between the inner chamber.Described metal weights body 2 is sealed in the rear end of freezing tubule 1 fully, and being convenient to freezing tubule can sink.In addition, metal weights body 2 can also accelerate the effect that heat is transmitted, and improves freezing efficiency, has reduced the freezing injury of sample.
Embodiment two: referring to Fig. 3, Fig. 4 and Fig. 5, content and embodiment one are basic identical, and something in common does not repeat, and different is: the freezing tubule sidewall of metal weights body 2 contacts is provided with hole 6.And, the front end of described metal weights body 2 is provided with forked muscle 21, described forked muscle 21 passes to stretch into behind the interlayer and deposits in the inner chamber 11, and applying is fixed on the madial wall of freezing tubule 1, forked muscle 21 not only has reinforcement and support effect to supporting freezing tubule, and be connected with the metal weights body and have the effect of accelerating cooling, cooling-down effect is remarkable.
Embodiment three: referring to Fig. 6, content and embodiment two are basic identical, something in common does not repeat, different is: described metal weights body 2 exposes to outside the freezing tubule, metal weights body 2 only has part to fix with freezing tubule 1 suit, the front end of metal weights body 2 is provided with forked muscle 21, and described forked muscle 21 passes to stretch into behind the interlayer 3 and deposits in the inner chamber 11, and fits and be fixed on the madial wall of freezing tubule 1.The metal weights body 2 that leaks outside is more conducive to conductive force.
Claims (3)
1. a mammal embryo and egg mother cell vitrified frozen vector, it is characterized in that: comprise freezing tubule and taper suction nozzle, both mate the make-up installation, the rear end of wherein said freezing tubule is equipped with the metal weights body, and depositing of this metal weights body and freezing tubule is provided with sealed compartment between the inner chamber; The front end of described freezing tubule and taper suction nozzle junction are provided with at least one sheared edge longitudinally; Described metal weights is external to be exposed to outside the freezing tubule, the metal weights body only has part to fix with freezing tubule suit, the front end of metal weights body is provided with forked muscle, and described forked muscle passes to stretch into behind the interlayer and deposits in the inner chamber, and fits and be fixed on the madial wall of freezing tubule.
2. mammal embryo according to claim 1 and egg mother cell vitrified frozen vector, it is characterized in that: described metal weights body is sealed in the rear end of freezing tubule fully, and perhaps, the freezing tubule sidewall of metal weights body contact is provided with the hole.
3. mammal embryo according to claim 1 and 2 and egg mother cell vitrified frozen vector, it is characterized in that: the bore scope of the most advanced and sophisticated incision of described taper suction nozzle is 100 μ m～200 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210172951 CN102669088B (en) | 2012-05-30 | 2012-05-30 | Mammal embryo and oocyte vitrification freezing carrier |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210172951 CN102669088B (en) | 2012-05-30 | 2012-05-30 | Mammal embryo and oocyte vitrification freezing carrier |
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| Publication Number | Publication Date |
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| CN102669088A CN102669088A (en) | 2012-09-19 |
| CN102669088B true CN102669088B (en) | 2013-08-14 |
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| CN 201210172951 Expired - Fee Related CN102669088B (en) | 2012-05-30 | 2012-05-30 | Mammal embryo and oocyte vitrification freezing carrier |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014201694A1 (en) * | 2013-06-21 | 2014-12-24 | Xu Xiaoyang | Automatic operation method and system for rapid vitrification freezing of living cells |
| CN104642299A (en) * | 2013-11-21 | 2015-05-27 | 浙江星博生物科技有限公司 | Vitrification freezing carrier system |
| CN104642298A (en) * | 2013-11-21 | 2015-05-27 | 浙江星博生物科技有限公司 | Miniature loading system for cryopreservation of biological materials |
| CN104782615A (en) * | 2015-04-17 | 2015-07-22 | 万超 | Closed super-fast vitrification refrigerating carrier, carrying rod and bevel refrigerating inserting component |
| CN107836446A (en) * | 2017-10-31 | 2018-03-27 | 江西易通医疗器械有限公司 | Cryopreservation tube |
| CN110214776A (en) * | 2019-07-01 | 2019-09-10 | 安徽农业大学 | It is a kind of based on straw freezing Tip carrier pig blastocyst freeze and defreezing method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1774501A (en) * | 2003-04-15 | 2006-05-17 | 株式会社北里萨普利 | Implement and method for freezing and storing egg |
| CN101200706A (en) * | 2007-12-21 | 2008-06-18 | 安徽医科大学 | Biological sample glass freezing and conserving appliance |
| CN101589707A (en) * | 2009-04-23 | 2009-12-02 | 北京微创介入医疗装备有限公司 | Embryo and ovum freezing carrier device for vitrifying in the auxiliary procreation technology |
| EP2353383A2 (en) * | 2010-02-09 | 2011-08-10 | Enrique Criado Scholz | Closed ultra-rapid cell vitrification device and sealing procedure of the device |
| CN102405901A (en) * | 2010-09-21 | 2012-04-11 | 北京微创介入医疗装备有限公司 | Suction type glass vitrification freezing carrier device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4373025B2 (en) * | 2001-04-18 | 2009-11-25 | 株式会社北里サプライ | Egg cryopreservation tool and cylindrical member holding device |
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2012
- 2012-05-30 CN CN 201210172951 patent/CN102669088B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1774501A (en) * | 2003-04-15 | 2006-05-17 | 株式会社北里萨普利 | Implement and method for freezing and storing egg |
| CN101200706A (en) * | 2007-12-21 | 2008-06-18 | 安徽医科大学 | Biological sample glass freezing and conserving appliance |
| CN101589707A (en) * | 2009-04-23 | 2009-12-02 | 北京微创介入医疗装备有限公司 | Embryo and ovum freezing carrier device for vitrifying in the auxiliary procreation technology |
| EP2353383A2 (en) * | 2010-02-09 | 2011-08-10 | Enrique Criado Scholz | Closed ultra-rapid cell vitrification device and sealing procedure of the device |
| CN102405901A (en) * | 2010-09-21 | 2012-04-11 | 北京微创介入医疗装备有限公司 | Suction type glass vitrification freezing carrier device |
Non-Patent Citations (1)
| Title |
|---|
| JP特开2002-315573A 2002.10.29 |
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| CN102669088A (en) | 2012-09-19 |
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