CN201990684U - Biological material vitrification cryopreservation load-bearing tool - Google Patents
Biological material vitrification cryopreservation load-bearing tool Download PDFInfo
- Publication number
- CN201990684U CN201990684U CN2011201100348U CN201120110034U CN201990684U CN 201990684 U CN201990684 U CN 201990684U CN 2011201100348 U CN2011201100348 U CN 2011201100348U CN 201120110034 U CN201120110034 U CN 201120110034U CN 201990684 U CN201990684 U CN 201990684U
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- sheath
- biological material
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- 239000012620 biological material Substances 0.000 title claims abstract description 20
- 238000005138 cryopreservation Methods 0.000 title abstract description 8
- 238000004017 vitrification Methods 0.000 title abstract 5
- 238000007789 sealing Methods 0.000 claims abstract description 12
- 230000008602 contraction Effects 0.000 claims description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 38
- 239000007788 liquid Substances 0.000 abstract description 20
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 19
- 239000012472 biological sample Substances 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 description 21
- 230000008014 freezing Effects 0.000 description 21
- 210000001161 mammalian embryo Anatomy 0.000 description 13
- 239000010902 straw Substances 0.000 description 10
- 239000011521 glass Substances 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 8
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 230000009977 dual effect Effects 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 238000001816 cooling Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 239000002577 cryoprotective agent Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 102000029749 Microtubule Human genes 0.000 description 1
- 108091022875 Microtubule Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 210000004688 microtubule Anatomy 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
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Abstract
The utility model relates to a biological material vitrification cryopreservation load-bearing tool, which comprises an inserting rod body and a sheath. The insert-rod body is an integral straight rod structure which consists of a handle, an inserting-connecting body and a carrier, an inserting groove is arranged at the position of a step of joint between the inserting-connecting body and the rear handle, and an annular protrusion is arranged at the end portion of the inserting-connecting body circumferentially. The sheath is sleeved on the inserting-connecting body and the carrier, the front end of the sheath is closed, the opening end of the rear portion of the sheath is inserted into the inserting groove tightly and integrally, and the inner wall of the sheath is closely matched with the annular protrusion of the inserting-connecting body. The inserting rod body is in sealed connection with the sheath by using inside and outside squeeze, and the flaky carrier bearing biological materials is reliably sealed inside the sheath. The biological material vitrification cryopreservation load-bearing tool is simple in structure, reliable in sealing, capable of isolating liquid nitrogen, completely avoiding potential biological pollution hazard and realizing vitrification cryopreservation of the biological materials, high in cryopreservation efficiency and survival rate of biological samples, good in using effects, and is an ideal biological material vitrification cryopreservation load-bearing tool.
Description
Technical field
The utility model belongs to biomedical sector, and specifically a kind of biological material vitrified frozen is preserved carrying tool.
Background technology
The application of glass freezing technology in human oocyte and embryo's freezing preservation in recent years increases gradually.The glass freezing technology is the freezing carrying tool that utilizes volume small; in the process of extremely fast cooling (〉 2000 ℃/min); the frozen solution that makes high density changes very high similar hyaloid solid-state of viscosity into by liquid state; avoid the formation of ice crystal in the cell; reach the effect of cryoprotection, thereby significantly improve viability and the developmental potency of cell/tissue after freezing.This technology is the important component part of embryo biotechnologies such as in vitro fertilization, microinjection, transgenosis and clone.In addition, the embryo cryopreservation technology can also be protected endangered animal, can make the high kind of utility value avoid suffering potential such as natural disaster, genetic drift, transmissible disease to threaten.The glass freezing technology has a lot of outstanding advantages, and substep adds antifreezing agent and freezes the loaded down with trivial details step that the back substep removes antifreezing agent before need not to freeze, and has simplified refrigerating process.Its operation steps mainly comprises: the inside and outside solution of cryoprotectant displacement cell of high density, fast cooling makes the solution stickiness extremely improve and solidify, and places liquid nitrogen and preserves.The supper-fast Refrigeration Technique of vitrifying is that it is the most succinct, the technology of embryo cryopreservation preservation the most fast, and refrigerating process is no more than 1 min, does not need expensive program control freezing instrument, is one of gordian technique that promotes the embryo transfer industrialization.The factor that influences the glass freezing effect mainly comprises: cryoprotectant, temperature lowering speed, embryo's carrier.To the requirement of embryo's carrier is that freezing preservatives volume around the embryo should be as far as possible little, few as far as possible at interval with liquid nitrogen, easy and simple to handle and be difficult for losing the embryo.
Present existing vitrified frozen vector, CN10386814A discloses a kind of biological material vitrified frozen vector and preparation method thereof as the Chinese patent publication number, and it comprises injection needles (1) and oatmeal (2); Described injection needles (1) is made up of syringe needle (1-1) and needle holder (1-2); Described oatmeal (2) is opening drawing-down straw, and one end (2-1) is sheathed in the inner chamber of described syringe needle (1-1); The straw wall part disappearance of another free end (2-2), the cross section of remaining straw wall is the 1/5-1/3 of circumference.Its weak point is: injection needles (1) directly is exposed in the liquid nitrogen with opening drawing-down straw, has potential biological pollution dangerous problem.
And for example Chinese patent publication number CN101200706A discloses biological sample glass freezing and conserving appliances such as ovum and embryo, comprises straw and protecting pipe, and protecting pipe is set on the straw; The straw middle part is very thin thin-walled tube, and very thin thin-walled tube length is 20-30MM, and diameter is Φ 0.3-0.35MM; Protecting pipe middle part zygomorphy is provided with the indent window.This straw instrument is used for the glass freezing of ovum or embryo and other cells to be preserved, and can make biological sample directly not be exposed to liquid nitrogen to prevent potential biological pollution danger.Yet above-mentioned freezing in use will in time the straw two ends being used with conserving appliance heated or the sealing member plug for seal, carry out glass freezing again, certainly will not reach the purpose of the fast cooling of expection; Cut off the straw two ends after thawing again, then biological sample could be released.Frozen efficient and result of use have been had a strong impact on.
Summary of the invention
Technical problem to be solved in the utility model is to overcome above-mentioned the deficiencies in the prior art, provide a kind of rational in infrastructure, make up easy, sealing is reliable, can isolate liquid nitrogen fully, avoid potential biological pollution danger, realize that the biological sample rapid glassization is freezing, biological material vitrified frozen easy for operation is preserved carrying tool.
The technical scheme in the invention for solving the above technical problem is: a kind of biological material vitrified frozen is preserved carrying tool, it is characterized in that: it comprises inserted link main body and sheath, the integrated straight-bar structure that described inserted link main body is made up of handle, grafting body, carrier, grafting body and back handle engage the step place and are provided with slot, grafting body root is circumferentially with annular protrusion, described sheath is enclosed within grafting body and carrier outside, its front end sealing, rear open end integral body closely is plugged in the slot, inwall and grafting body annular protrusion wringing fit.
The said slot of the utility model has the contraction tapering, and wherein the handle front inner wall is provided with enlarging forward.The inserted link main body cooperates with sheath when pegging graft, and is the inside and outside dual extruding between inserted link main body and the sheath, connects sealing.
The said carrier of the utility model is a sheet, also is provided with a through hole in the middle of the chip carrier.Be convenient to load biological samples such as ovum and embryo.
The said sheath front end of the utility model also is equipped with counterweight in the inside.Be in upright plumbness so that it puts into the liquid nitrogen operating case, be convenient to the inserted link main body and insert in the sheath.
The utility model is owing to adopt the inside and outside dual extruding between inserted link main body and the sheath, connect sealing, the chip carrier of carrying biomaterial is sealed in the sheath reliably, complete and liquid nitrogen is isolated, avoided biological sample by the virus and bacteria in the liquid nitrogen and other pathogen contamination, can make biological sample realize that rapid glassization is freezing again.Against existing technologies, the utility model is rational in infrastructure, makes up easyly, and sealing is reliable, can isolate liquid nitrogen fully, avoid potential biological pollution danger, realize that the biological sample rapid glassization is freezing, it is easy for operation, good airproof performance, frozen efficient height, result of use is good, the surviving rate height of biological sample.Be that a kind of ideal biological material vitrified frozen is preserved carrying tool.
Description of drawings
Below in conjunction with accompanying drawing the utility model is described further.
Fig. 1 is a structural representation of the present utility model.
Fig. 2 is the structural representation of the utility model inserted link main body.
Fig. 3 is the A portion enlarged diagram of Fig. 1.
1. inserted link main bodys among the figure, 2. sheath, 3. counterweight, 11. handles, 12. grafting bodies, 13. carriers, 121. slots, 122. annular protrusions, 131. through holes.
Embodiment
As can be seen, a kind of biological material vitrified frozen is preserved carrying tool from Fig. 1, Fig. 2, Fig. 3, and it comprises inserted link main body 1 and sheath 2, the integrated straight-bar structure that described inserted link main body 1 is made up of handle 11, grafting body 12, carrier 13.Handle 11 is positioned at that rear portion, grafting body 12 are positioned at the middle part, carrier 13 is positioned at the front portion, is the straight-bar stepped profile.Grafting body 12 and back handle 11 engage the step place and are provided with slot 121, grafting body 12 roots are circumferentially with annular protrusion 122, described sheath 2 is enclosed within grafting body 12 and carrier 13 outsides, its front end sealing, rear open end integral body closely is plugged in the slot 121, inwall and grafting body annular protrusion 122 wringing fits.
The said slot 121 of the utility model has the contraction tapering, and wherein back handle 11 front inner wall are provided with enlarging forward.As shown in Figure 3.Inserted link main body 1 cooperates with sheath 2 when pegging graft, and sheath 2 rear open end and grafting body 12 annular protrusions 122 wringing fits expand outwardly; Simultaneously, by the sheath 2 rear open end outside of the outer support of annular protrusion 122 again with slot 121 wringing fits of band tapering, inwardly shrink extruding, like this, be the inside and outside dual extruding between inserted link main body 1 and the sheath 2, connect and seal.Its grafting is easy, connection, airtight reliable.
The said carrier 13 of the utility model is a sheet, also is provided with a through hole 131 in the middle of the chip carrier 13.Be convenient to load biological samples such as ovum and embryo.
The said sheath of the utility model also is equipped with counterweight 3 in the 2 front end inside.Be in upright plumbness so that it puts into the liquid nitrogen operating case, be convenient to inserted link main body 1 and insert in the sheath 2.
Adopt the inside and outside dual extruding between the utility model inserted link main body and the sheath, connect sealing, the chip carrier of carrying biomaterial is sealed in the sheath reliably, complete and liquid nitrogen is isolated, avoided biological sample by the virus and bacteria in the liquid nitrogen and other pathogen contamination, can make biological sample realize that rapid glassization is freezing again.
The concrete freezing preservation use of the utility model is: handle 11 marker number and patient information in inserted link main body 1 are affixed on handle 11 positions with label.The through hole 131 of the chip carrier 13 of inserted link main body 1 is moved in the field of microscope, and by microtubule ovum or blastaea etc. being injected through hole 131 is on the carrier 13 at center.Before glass freezing begins, place refrigeration operation box liquid nitrogen more than at least 30 seconds sheath 2, and be fixed on the anchor of operating case.Then fast with in the inserted link main body 1 vertical sheath 2 that inserts in the liquid nitrogen box, inserted link main body 1 cooperates grafting, sealing with sheath 2, and the envelope of maintaining secrecy is intact, carries out glass freezing fast and preserves, and biological sample directly is not exposed in the liquid nitrogen.Take off sheath 2 and inserted link main body 1 on the anchor that is fixed on then, and they are being moved in liquid nitrogen in the existing freeze pipe, so that in the medium-term and long-term preservation of liquid nitrogen container.
The utility model is rational in infrastructure, makes up easyly, and sealing is reliable, can isolate liquid nitrogen fully, avoid potential biological pollution danger, realize that the biological sample rapid glassization is freezing, it is easy for operation, good airproof performance, frozen efficient height, result of use is good, the surviving rate height of biological sample.Be that a kind of ideal biological material vitrified frozen is preserved carrying tool.
Claims (4)
1. a biological material vitrified frozen is preserved carrying tool, it is characterized in that: it comprises inserted link main body and sheath, the integrated straight-bar structure that described inserted link main body is made up of handle, grafting body, carrier, grafting body and back handle engage the step place and are provided with slot, grafting body root is circumferentially with annular protrusion, and described sheath is enclosed within grafting body and carrier outside, its front end sealing, rear open end integral body closely is plugged in the slot, inwall and grafting body annular protrusion wringing fit.
2. preserve carrying tool according to the described biological material vitrified frozen of claim 1, it is characterized in that said slot has the contraction tapering, wherein the handle front inner wall is provided with enlarging forward.
3. preserve carrying tool according to the described biological material vitrified frozen of claim 1, it is characterized in that said carrier is a sheet, also be provided with a through hole in the middle of the chip carrier.
4. preserve carrying tool according to the described biological material vitrified frozen of claim 1, it is characterized in that in the said sheath front end inside counterweight being installed also.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201100348U CN201990684U (en) | 2011-04-15 | 2011-04-15 | Biological material vitrification cryopreservation load-bearing tool |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201100348U CN201990684U (en) | 2011-04-15 | 2011-04-15 | Biological material vitrification cryopreservation load-bearing tool |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201990684U true CN201990684U (en) | 2011-09-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011201100348U Expired - Lifetime CN201990684U (en) | 2011-04-15 | 2011-04-15 | Biological material vitrification cryopreservation load-bearing tool |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201990684U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104412961A (en) * | 2013-08-22 | 2015-03-18 | 薛松果 | Biological sample vitrification freezing and preserving apparatus |
| CN104642297A (en) * | 2013-11-21 | 2015-05-27 | 浙江星博生物科技有限公司 | Liquid nitrogen filter unit for sealed cryopreservation of biological materials |
| CN105230605A (en) * | 2015-09-21 | 2016-01-13 | 南宁市第二人民医院 | Vitrification frozen carrier |
| CN105705626A (en) * | 2013-10-29 | 2016-06-22 | 学校法人北里研究所 | Tool for vitrifying cryopreservation of cells or tissue |
-
2011
- 2011-04-15 CN CN2011201100348U patent/CN201990684U/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104412961A (en) * | 2013-08-22 | 2015-03-18 | 薛松果 | Biological sample vitrification freezing and preserving apparatus |
| CN105705626A (en) * | 2013-10-29 | 2016-06-22 | 学校法人北里研究所 | Tool for vitrifying cryopreservation of cells or tissue |
| CN105705626B (en) * | 2013-10-29 | 2018-11-16 | 学校法人北里研究所 | The vitrificated cryopreserration tool of cell or tissue |
| CN104642297A (en) * | 2013-11-21 | 2015-05-27 | 浙江星博生物科技有限公司 | Liquid nitrogen filter unit for sealed cryopreservation of biological materials |
| CN105230605A (en) * | 2015-09-21 | 2016-01-13 | 南宁市第二人民医院 | Vitrification frozen carrier |
| CN105230605B (en) * | 2015-09-21 | 2017-11-10 | 南宁市第二人民医院 | Vitrified frozen vector |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20110928 |
|
| CX01 | Expiry of patent term |