CN105230605B - Vitrified frozen vector - Google Patents
Vitrified frozen vector Download PDFInfo
- Publication number
- CN105230605B CN105230605B CN201510601902.5A CN201510601902A CN105230605B CN 105230605 B CN105230605 B CN 105230605B CN 201510601902 A CN201510601902 A CN 201510601902A CN 105230605 B CN105230605 B CN 105230605B
- Authority
- CN
- China
- Prior art keywords
- carrier
- straw
- inner sleeve
- plug
- outer tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Medical Preparation Storing Or Oral Administration Devices (AREA)
Abstract
The present invention discloses a kind of vitrified frozen vector, including carrier, inner sleeve and outer tube, the carrier includes straw, carrier pipe and hand holding handle, straw is in medical needle-like, its front end is provided with Baltimore groove, and one section described of carrier pipe is openend, and the other end is closed end, the straw Baltimore groove is connected with the carrier pipe openend, and the carrier pipe closed end is connected with hand holding handle；Described inner sleeve includes plug and interior tube body, and described interior tube body one end is horn mouth, and the other end is umbrella shape, and centre is tubing string, and the plug includes syringe needle and handle face；Outer tube one end closing other end is opened, and the carrier is arranged in tubing string, and plug is connected with horn mouth, and inner sleeve is arranged in outer tube.Freezing carrier of the present invention reduces cost, and making material is conventional consumptive material, easily obtains, and simple to operate, according to the soakage of straw, the accurate amount for controlling freezing liquid.
Description
Technical field
The present invention relates to technical field of medical instruments, and in particular to a kind of vitrified frozen vector.
Background technology
Application of the vitrification in the freezen protective of human oocyte and embryo in recent years gradually increases.Vitrifying
Refrigeration Technique is using the small freezing carrying tool of volume, is extremely fast being cooled（>2000 ℃/min）During, make highly concentrated
The frozen solution of degree is changed into the very high similar hyaloid solid-state of viscosity by liquid, avoids the formation of intracellular ice crystal, reaches
To the effect of cryoprotection, so as to significantly improve viability and developmental potency after cell/tissue freezing.The technology be in vitro by
The important component of the embryo biotechnologies such as essence, microinjection, transgenosis and clone.In addition, Embryo Cryopreservation Technology can be with
Endangered animal is protected, it is latent that the kind that value can be made high protects against natural calamity, genetic drift, infectious disease etc.
Threat.Vitrification has the advantages of many prominent, without being taken off step by step after adding antifreeze and jelly before jelly step by step
Except the tedious steps of antifreeze, refrigerating process is simplified.Its operating procedure mainly includes：The cryoprotector displacement of high concentration is thin
Solution outside intracellular, fast cooling, solution stickiness is extremely improved and is solidified, place Liquid nitrogen storage.The ultrafast quickly cooling of vitrifying
Freeze techniques are its most succinct, most quick Embryo freezing preservation technologies, and refrigerating process is no more than 1 min, it is not necessary to expensive journey
Control freezing instrument, is one of key technology for promoting embryo transfer industrialization.The factor for influenceing glass freezing effect is mainly wrapped
Include：Cryoprotector, temperature drop rate, embryo's carrier.Requirement to embryo's carrier is the Cryoprotectant body around embryo
Product should be as far as possible small, as far as possible few with liquid nitrogen interval, and the present Refrigeration Technique of embryo easy to operate and not easy to be lost can be used in freezing
The embryo of sperm, ovum and different times, especially vitrification (Vitrification) are preserved, it has not only been obtained
Medical field is obtained widely to approve, and the slow art freezing of substitution tradition gradually, and as the optimal selection of Refrigeration Technique, even by this
Technology application is in highly difficult ovum, ovary tissue and the freezing of hESC.
In the selection of vitrified frozen vector, due to considering the factor of freezing rate, open freezing is commonly used, makes to contain
The vitrification solution of embryo directly contacts with liquid nitrogen, accelerates freezing rate.But it can not be ignored in refrigerating process and potential disease be present
Pathogenic microorganism, virus pollution.Load to solve existing vitrified frozen vector and the tubules of aluminium brackets is must be placed in after embryo
It is interior, it can just be stored in for a long time in the pail of liquid nitrogen container, liquid nitrogen container waste of storage space when causing aculea flyback cycle embryo cryopreservation
Problem, the utilization ratio for improving the effective memory space of liquid nitrogen container is urgent problem to be solved.
The content of the invention
The present invention is solves potential pathogenic microorganism, virus pollution during glass freezing, and freezing carrier is in liquid nitrogen
Middle floating, can not be intact preservation liquid the problems such as.To realize, technical scheme is used in the object of the invention：
A kind of vitrified frozen vector, including carrier, inner sleeve and outer tube, the carrier include straw, carrier pipe and
Hand holding handle, straw are in medical needle-like, and its front end is provided with Baltimore groove, and one section described of carrier pipe is openend, and the other end is closure
End, the straw Baltimore groove are connected with the carrier pipe openend, and the carrier pipe closed end is connected with hand holding handle；Described is interior
Sleeve pipe includes plug and interior tube body, and described interior tube body one end is horn mouth, and the other end is umbrella shape, and centre is tubing string, described stifled
Head includes syringe needle and handle face；Outer tube one end closing other end is opened, and the carrier is arranged in tubing string, plug and loudspeaker
Mouth connection, inner sleeve are arranged in outer tube.
Described handle face is rectangle, and handle face is used for label information.
Described straw is Baltimore groove, and spill slot length is 15-25mm.
Metal ball is set in described tubing string, and metal ball is connected with umbrella shape.
Described carrier lengths are 100-250mm, a diameter of 1-5mm.
Described carrier pipe sets 2-5 semicircle necks.
Beneficial effect of the present invention：Cost is reduced, making material is conventional consumptive material, is easily obtained, and simple to operate, according to wheat
The soakage of pipe, the accurate amount for controlling freezing liquid, using metal ball, sample sinks to liquid nitrogen, and metal ball also has good lead
Hot property, make sample snap frozen, reduce the freezing injury of sample.Made marks on handle face, be easy to preserve sample for a long time and obtain
Know relevant information.Totally-enclosed biological sample, prevents sample from directly being contacted with liquid nitrogen, avoids biological sample micro- by potential cause of disease
Biology, virus pollution.
Brief description of the drawings
Fig. 1 is vitrified frozen vector schematic diagram of the present invention；
Fig. 2 is inner sleeve schematic side view of the present invention；
Fig. 3 is interior tube body schematic diagram of the present invention；
Fig. 4 is carrier schematic diagram of the present invention；
Fig. 5 is carrier pipe schematic diagram of the present invention；
Fig. 6 is plug schematic diagram of the present invention；
Parts shown in figure and sequence number：
1st, outer tube, 2, interior tube body, 3, plug, 4, horn mouth, 5, tubing string, 6, umbrella shape, 7 straws, 8, carrier pipe, 9, hand-held
Handle, 10, syringe needle, 11, handle face.
Embodiment
A kind of vitrified frozen vector, carrier lengths 100-250mm, a diameter of 1-5mm, including carrier, inner sleeve and
Outer tube, the carrier include straw 7, carrier pipe 8 and hand holding handle 9, and straw 7 is in medical needle-like, and its front end is provided with Baltimore groove, recessed
Shape slot length is 15-25mm；8 one sections described of carrier pipe is openend, and the other end is closed end, the Baltimore groove of straw 7 with
The openend of carrier pipe 8 connection, the closed end of carrier pipe 8 are connected with hand holding handle 9, and carrier pipe 8 sets 2-5 semicircle card
Groove；Described inner sleeve includes plug 3 and interior tube body 2, and described one end of interior tube body 2 is horn mouth 4, and the other end is umbrella shape 6, in
Between to set metal ball in tubing string 5, metal ball is connected with umbrella shape 6；The plug 3 includes syringe needle 10 and rectangular shank face 11, handle face
11 are used for label information；Outer tube one end closing other end is opened, and the carrier is arranged in tubing string 5, plug 3 and loudspeaker
Mouth 4 is connected, and inner sleeve is arranged in outer tube.
Claims (5)
1. a kind of vitrified frozen vector, including carrier, inner sleeve and outer tube（1）, it is characterised in that the carrier includes wheat
Pipe（7）, carrier pipe（8）And hand holding handle（9）, straw（7）In medical needle-like, its front end is provided with Baltimore groove, described carrier pipe（8）
One section is openend, and the other end is closed end, the straw（7）Baltimore groove and the carrier pipe（8）Openend connects, described to hold
Carry pipe（8）Closed end and hand holding handle（9）Connection；Described inner sleeve includes plug（3）And interior tube body（2）, described interior tube body
（2）One end is horn mouth（4）, the other end is umbrella shape（6）, centre is tubing string（5）, the plug（3）Including syringe needle（10）And handle
Face（11）；Outer tube one end closing other end opens, and the carrier is arranged on tubing string（5）It is interior, plug（3）With horn mouth
（4）Connection, inner sleeve are arranged on outer tube（1）It is interior；Described tubing string（5）Interior setting metal ball, metal ball and umbrella shape（6）Even
Connect.
2. vitrified frozen vector according to claim 1, it is characterised in that described handle face（11）For rectangle, handle face
（11）For label information.
3. vitrified frozen vector according to claim 1, it is characterised in that described straw（7）It is Baltimore groove, spill
Slot length is 15-25mm.
4. vitrified frozen vector according to claim 1, it is characterised in that described carrier lengths are 100-250mm,
A diameter of 1-5mm.
5. vitrified frozen vector according to claim 1, it is characterised in that described carrier pipe（8）Set 2-5 half
Circular neck.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510601902.5A CN105230605B (en) | 2015-09-21 | 2015-09-21 | Vitrified frozen vector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510601902.5A CN105230605B (en) | 2015-09-21 | 2015-09-21 | Vitrified frozen vector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105230605A CN105230605A (en) | 2016-01-13 |
| CN105230605B true CN105230605B (en) | 2017-11-10 |
Family
ID=55028675
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510601902.5A Active CN105230605B (en) | 2015-09-21 | 2015-09-21 | Vitrified frozen vector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105230605B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106342789B (en) * | 2016-10-13 | 2022-07-15 | 深圳韦拓生物科技有限公司 | Cell vitrification freezing treatment equipment and treatment method thereof |
| CN107535482A (en) * | 2017-10-11 | 2018-01-05 | 山东大学齐鲁医院 | A kind of bulk Vitrification of Ovarian Tissue carrier and its application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201284339Y (en) * | 2008-10-21 | 2009-08-05 | 北京大学第三医院 | Biological material vitrification freezing vector |
| CN201990684U (en) * | 2011-04-15 | 2011-09-28 | 山东威高新生医疗器械有限公司 | Biological material vitrification cryopreservation load-bearing tool |
| CN202514485U (en) * | 2011-12-29 | 2012-11-07 | 深圳市易瑞生物技术有限公司 | Carrying tool for vitrified cryopreservation of biological materials |
| CN205040512U (en) * | 2015-09-21 | 2016-02-24 | 南宁市第二人民医院 | Vitrification carrier |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100546721C (en) * | 2004-12-21 | 2009-10-07 | 麦吉尔大学 | The tool and method of operation developmental cells sample in process of cryopreservation |
-
2015
- 2015-09-21 CN CN201510601902.5A patent/CN105230605B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201284339Y (en) * | 2008-10-21 | 2009-08-05 | 北京大学第三医院 | Biological material vitrification freezing vector |
| CN201990684U (en) * | 2011-04-15 | 2011-09-28 | 山东威高新生医疗器械有限公司 | Biological material vitrification cryopreservation load-bearing tool |
| CN202514485U (en) * | 2011-12-29 | 2012-11-07 | 深圳市易瑞生物技术有限公司 | Carrying tool for vitrified cryopreservation of biological materials |
| CN205040512U (en) * | 2015-09-21 | 2016-02-24 | 南宁市第二人民医院 | Vitrification carrier |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105230605A (en) | 2016-01-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| He et al. | Vitrification by ultra-fast cooling at a low concentration of cryoprotectants in a quartz micro-capillary: a study using murine embryonic stem cells | |
| Vanderzwalmen et al. | Aseptic vitrification of blastocysts from infertile patients, egg donors and after IVM | |
| JP6475493B2 (en) | Germ cell preservation tool and germ cell preservation method | |
| CN103190393B (en) | Biological sample vitrification carrier and application thereof | |
| US20100196873A1 (en) | Systems and Methods for Cryopreservation of Cells | |
| US20080003561A1 (en) | Systems and Methods for Cryopreservation of Cells | |
| CN105230605B (en) | Vitrified frozen vector | |
| CN101386814B (en) | Biological material vitrified frozen vector and preparation method thereof | |
| Somfai et al. | Vitrification of porcine oocytes and zygotes in microdrops on a solid metal surface or liquid nitrogen | |
| Punyatanasakchai et al. | Comparison of cryopreserved human sperm in vapor and liquid phases of liquid nitrogen: effect on motility parameters, morphology, and sperm function | |
| CN203898495U (en) | Embryo transfer device for rat and mouse embryo transfer | |
| CN205040512U (en) | Vitrification carrier | |
| CN102669088A (en) | Mammal embryo and oocyte vitrification freezing carrier | |
| CN202514485U (en) | Carrying tool for vitrified cryopreservation of biological materials | |
| JP4431754B2 (en) | Vitrification preservation tool of embryo and vitrification preservation method of embryo using the same | |
| CN101589707A (en) | Embryo and ovum freezing carrier device for vitrifying in the auxiliary procreation technology | |
| CN203340879U (en) | Bearing tool for vitrified cryopreservation of biological material | |
| CN206949363U (en) | It is a kind of to be applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum | |
| CN201990684U (en) | Biological material vitrification cryopreservation load-bearing tool | |
| Nagy et al. | Cryopreservation of eggs | |
| Do et al. | Requirements for Cryopreservation of | |
| Fuller et al. | Cryopreservation of mammalian embryos | |
| CN206481877U (en) | A kind of hydrodynamics vitrified frozen vector | |
| CN202773904U (en) | Biological material vitrification freezing carrier | |
| CN105660605B (en) | A kind of efficient gamete/freeze-thaw method for vitrification of embryo |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |