CN107535482A - A kind of bulk Vitrification of Ovarian Tissue carrier and its application - Google Patents
A kind of bulk Vitrification of Ovarian Tissue carrier and its application Download PDFInfo
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- CN107535482A CN107535482A CN201710942650.1A CN201710942650A CN107535482A CN 107535482 A CN107535482 A CN 107535482A CN 201710942650 A CN201710942650 A CN 201710942650A CN 107535482 A CN107535482 A CN 107535482A
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Abstract
The invention discloses a kind of bulk Vitrification of Ovarian Tissue carrier is provided, the carrier is made up of carrier inner core and outer tube, and the carrier inner core is threadedly coupled with outer tube, has both kept good sealing, prevents the cross-infection of ovary tissue；User's operation is also convenient for simultaneously；The carrier inner core is from top to bottom made up of Handheld Division, cap portion, support sector and supporting part successively, and the supporting part is gripped by support sector；In use, bulk ovary tissue is disposed away from an end of the supporting part of support sector, the end is bulk ovary tissue loading area；Experiments verify that using carrier of the present invention, refrigerant osmotic efficiency, and bulk storage that is simple to operate, cheap, being easy to bulk ovary tissue are not only improved, suitable for popularization and application.
Description
Technical field
The invention belongs to medical instruments field, and in particular to a kind of bulk Vitrification of Ovarian Tissue carrier and its should
With.
Background technology
Ovary tissue freezen protective is most fast and most effective preservation fertility for the adult female for treating chemicotherapy treatment
Method, for prepuberal women, ovary tissue freezing is to preserve the unique channel of fecundity.Traditional ovary group
Knitting Refrigeration Technique takes sequencing to freeze, and has helped several women to obtain the child of oneself by the technology at present.But program
Change Refrigeration Technique because the shortcomings of instrument price is expensive, cooling time is long, cumbersome limits the clinical expansion of ovarian freezing technology.
Vitrification is the trend of following cell/tissue cryogenic freezing, possesses that simple to operate, cost is cheap, freezing efficiency is high
Etc. advantage, sequencing freezing substantially completely instead of in human oocyte and Embryo freezing preservation field at present.Vitrifying
Refrigeration Technique is to utilize cell/tissue being put into special freezing carrier, direct plunge into Liquid Nitrogen, makes cryoprotector moment by liquid
It is changed into hyaloid solid-state, avoids forming ice crystal into the cell.
Although glass freezing gradually starts to be applied to ovary tissue freezing region, freezing tissue volume maintains mostly
In 1mm × (1-5) mm × (1-5) mm, its main cause is that tissue block crosses conference and causes the cryoprotector short time not to be impregnated with
Middle section, and the exposure of long-time refrigerant can cause serious cytotoxic damage.Fritter ovary tissue (≤50mm3) due to
Only contain a small amount of ovarian follicle in transplanting tissue, endocrine function is held time shorter (1-3) after transplanting.And bulk ovary cortex
(≥80mm3) because containing abundant primordial follicle stock, hormone can be made to hold time longer, preserve and lead in female fertility
Domain has broad application prospects.Therefore, ovary middle section is protected during how realizing bulk Vitrification of Ovarian Tissue
The abundant infiltration for protecting agent is successful key.
Freezing carrier is the device of vitrifying carrying cell/tissue, is one of successful key factor of glass freezing.Mesh
The vitrified frozen vector that preceding ovarian freezing uses mainly includes freezing straw, freezing ring, cell screen clothes, aculeus type carrier etc.,
Wherein freeze straw and freeze the ovary tissue that ring is only capable of loading smaller size smaller, aculeus type carrier can cause ovary tissue irreversible
Mechanical damage, occupancy volume is excessive when cell screen clothes are put into liquid nitrogen container storage, is not easy bulk storage.Therefore, above-mentioned carrier is in ovum
The clinical practice of nest tissue freezen protective faces many difficulties in promoting, and is not yet widely accepted.Current ovary tissue on the market
The Ova Cryo Device Type M carriers of the rarely seen KITAZATO companies of carrier, not only expensive (about 300 yuan /), and
It is easy to fall off and draw point and cortex contact area are small in ovary tissue loading process, it is difficult to popularize in clinical and research work.
The content of the invention
For the deficiency of the design of the above-mentioned Vitrification of Ovarian Tissue carrier of bulk in the prior art, inventor is through long-term
Technology and practical exploration, there is provided a kind of simple efficient, cheap bulk study on vitrification of ovary carrier.Through testing
Card, using carrier of the present invention, not only improve refrigerant osmotic efficiency, and it is simple to operate, cheap, be easy to bulk ovum
The bulk storage of nest tissue, suitable for popularization and application.
Specifically, the present invention relates to following technical scheme：
The first aspect of the invention, there is provided a kind of bulk Vitrification of Ovarian Tissue carrier, the carrier is by carrying
Internal core and outer tube composition, the carrier inner core are threadedly coupled with outer tube, have both kept good sealing, prevented ovary group
The cross-infection knitted；User's operation is also convenient for simultaneously；
The carrier inner core is from top to bottom made up of Handheld Division, cap portion, support sector and supporting part successively, the supporting part by
Support sector grips；In use, bulk ovary tissue is disposed away from an end of the supporting part of support sector, the end is
Bulk ovary tissue loading area；
Preferably, the supporting part is metallic sieve, it is further preferred that the metallic sieve be 80-120 mesh 304 not
Become rusty steel metal screen cloth, and the present invention especially selects the stainless steel metallic sieve of specific mesh number on the one hand can as supporting part
Meet infiltration demand of the cryoprotector to ovary tissue, while take into account the soft durometer of ovarian freezing carrier, facilitate ovary tissue
Cryopreservation operates, while it also has the advantage such as cheap, non-corrosive, sturdy and durable；
Preferably, the carrier inner core is provided with sealing ring with outer tube junction, so as to strengthen sealing function, avoids ovum
The cross-infection of nest tissue；
Preferably, the Handheld Division diameter is less than cap portion diameter, and convenient use person takes, it is further preferred that the hand
The portion of holding is provided with multiple anti-skid bulges, and the user that is more convenient for takes；
Preferably, support sector's external diameter is less than the internal diameter of outer tube, and carrier inner core in support sector by setting
External screw thread is threadedly coupled with the internal thread set on outer tube with outer tube；
Preferably, the outer sleeve bottom is provided with one or more steady rest, carrier inclined when preventing preservation.
The second aspect of the invention, the carrier is disclosed in Vitrification of Ovarian Tissue and vitrifying recovery
Using.
Specifically, the ovary tissue is bulk ovary tissue, it is preferred that the bulk ovary tissue volume be 1mm ×
(0.5-1)cm×(0.8-1.5)cm；The present invention is by well-designed, so as to improve Vitrification of Ovarian Tissue protective agent
Permeability, glass freezing efficiency is improved, so as to realize the glass freezing to bulk ovary tissue.
It is described application specific method be：
Glass freezing：The bulk ovary tissue first equilibrium at room temperature in equilibrium liquid is subsequently placed in glass freezing liquid
Freezing processing is for a period of time；After ovary tissue surface liquid is blotted, hold Handheld Division and bulk ovary tissue is laid in supporting part
Loading area is organized, carrier cap portion is placed in liquid nitrogen with lower portion rapidly after loading and stirred, then carrier inner core is put into and filled
Have in the outer tube in liquid nitrogen, and tighten, be put into freezing storing box and preserve for a long time；
Vitrifying is recovered：Carrier is taken out from freeze box, outwards winding carrier inner core on liquid nitrogen surface, holding Handheld Division will tile
Ovary tissue move into warmed-up resuscitation fluid I and balance, then ovary tissue is moved into resuscitation fluid II and carries out equilibrium at room temperature, most
After move in G-mops treatment fluids balance and complete vitrifying recovery, hormone determination after can then carrying out morphological analysis and cultivating.
Wherein, equilibrium liquid component is：The ethylene glycol of the dimethyl sulfoxide (DMSO) of basal liquid+7.5%+7.5%；
Glass freezing liquid component is：Ethylene glycol+0.5M the sucrose of the dimethyl sulfoxide (DMSO) of basal liquid+20%+20%；
Resuscitation fluid I components are：Basal liquid+1M sucrose；
Resuscitation fluid II components are：Basal liquid+0.5M sucrose；
Above-mentioned basal liquid component is the human serum albumins of G-mops treatment fluids+5% (HSA)；
Compared with prior art, the present invention has the beneficial effect that：
(1) cost is low, simple to operate, bulk Vitrification of Ovarian Tissue carrier the selection of material source prepared by the present invention
Extensively, price is low；Operating process can operate at room temperature, avoid in traditional freezing using Slow-rate freezing instrument high cost and
Complicated procedures；
(2) carrier outer tube is mounted with liquid nitrogen in the present invention so that liquid nitrogen and the ovary tissue being fixed on metallic sieve
Directly contact, and metallic sieve has good heat conductivility, so as to accelerate chilling rate, extends cooling time, and reduce sample
The cooling time of product；Simultaneously, it is necessary to illustrate, liquid nitrogen is placed in outer tube in the present invention, when carrier inner core leads to outer tube
After crossing threaded connection closure, i.e., form autonomous closure with ovary tissue and contact, avoid ovary tissue by other potential cause of diseases
Microorganism, virus pollution, and during closure progress freezen protective, the metallic sieve after screwing maintains stationary state, prevents ovary
Tissue sample is because rocking disengaging wire netting, so as to more facilitate ovary tissue sample access；
(3) carrier operation of the present invention is used, the cryoprotector volume of ovary tissue surface attachment can be down to most
It is low, so as to effectively improve rate of temperature fall and reduce the adverse effect of glass freezing liquid residual, simultaneously as glass freezing drops
Warm speed is significantly improved, therefore the glass freezing liquid of low concentration can be used to carry out dewater treatment, therefore further drop
Reduced TG freezing liquid risk of toxicity；
(4) carrier of the present invention and application method are used, can well preserve the form and vigor of ovarian follicle, while this hair
The carrier of bright design can be with the relatively large ovary tissue of directly freezed, so as to further increase ovary tissue survival rate；
(5) design vector of the present invention reduces ovary tissue freezing on the basis of not influenceing or even increasing ease of use
Shared space, floor space needed for stored frozen is greatly saved, is easy to bulk storage, suitable for building for ovary tissue storehouse
Vertical and clinical application.
Brief description of the drawings
Fig. 1 is freezing carrier model core arrangement schematic diagram in the present invention, and wherein Fig. 1 (a) is front view, and Fig. 1 (b) is side view
Figure；
Fig. 2 is freezing carrier inner core upward view in the present invention；
Fig. 3 is freezing carrier outer tube profile in the present invention；
Fig. 4 is freezing carrier outer tube upward view in the present invention；
Fig. 5 is using morphologic observation figure after present invention progress bulk Vitrification of Ovarian Tissue recovery；
Fig. 6 is that hormonal readiness determines after carrying out bulk Vitrification of Ovarian Tissue recovery using the present invention.
Wherein, 1- Handheld Divisions, 2- caps portion, 3- sealing rings, 4- support sectors, 5- external screw threads, 6- supporting parts, 7- ovary tissues dress
Carry area, 8- internal threads, 9- outer tubes, 10- steady rests.
Embodiment
It is noted that described further below is all exemplary, it is intended to provides further instruction to the application.It is unless another
Indicate, all technologies used herein and scientific terminology are with usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singulative
It is also intended to include plural form, additionally, it should be understood that, when in this manual using term "comprising" and/or " bag
Include " when, it indicates existing characteristics, step, operation, device, component and/or combinations thereof.
In the present invention, term as " on ", " under ", "left", "right", "front", "rear", " vertical ", " level ", " side ",
The orientation or position relationship of instructions such as " bottoms " are based on orientation shown in the drawings or position relationship, only to facilitate describing this hair
Bright each part or component structure relation and the relative determined, not refer in particular to either component or element in the present invention, it is impossible to understand
For limitation of the present invention.
As background technology is introduced, the vitrified frozen vector used in the prior art ovarian freezing mainly includes
Straw, freezing ring, cell screen clothes, aculeus type carrier are freezed, but generally existing freezing ovary tissue is too small, and refrigerating effect is bad,
There is damage to ovary tissue, the various problems such as price is high；
In view of this, in an exemplary embodiment of the invention, there is provided a kind of bulk Vitrification of Ovarian Tissue carries
Body, the carrier are made up of carrier inner core and outer tube (9), and the carrier inner core is threadedly coupled with outer tube (9)；
The carrier inner core is from top to bottom made up of Handheld Division (1), cap portion (2), support sector (4) and supporting part (6) successively,
The supporting part (6) is gripped by support sector (4)；In use, bulk ovary tissue is disposed away from the supporting part of support sector (4)
(6) a end, the end are bulk ovary tissue loading area (7)；
Wherein, the supporting part (6) is metallic sieve, it is further preferred that the metallic sieve be 80-120 mesh 304 not
Become rusty steel metal screen cloth；
The carrier inner core is provided with sealing ring (3) with outer tube (9) junction, so as to strengthen sealing function, avoids ovum
The cross-infection of nest tissue；
Handheld Division (1) diameter is less than cap portion (2) diameter, and the Handheld Division (1) is provided with multiple anti-skid bulges, more just
Taken in user；
Support sector (4) external diameter is less than the internal diameter of outer tube (9), and carrier inner core is by being set in support sector (4)
External screw thread (5) be threadedly coupled with the internal thread (8) set on outer tube (9) with outer tube；
The outer sleeve bottom is provided with one or more steady rest (10), carrier inclined when preventing preservation.
In the still another embodiment of the present invention, the carrier is disclosed in Vitrification of Ovarian Tissue and vitrifying
Application in recovery.
Specifically, the ovary tissue is bulk ovary tissue, the bulk ovary tissue volume is 1mm × (0.5-1)
cm×(0.8-1.5)cm；
It is described application specific method be：
Glass freezing：Ovary tissue is cut into 1mm × (0.5-1) cm × (0.8-1.5) cm size tissue blocks, in equilibrium liquid
Middle equilibrium at room temperature 25min, the 15min in glass freezing liquid；After ovary tissue surface liquid is blotted, holding Handheld Division (1) will
Bulk ovary tissue is laid in supporting part (6) ovary tissue loading area (7), rapidly by carrier cap portion (1) with lower portion after loading
It is placed in liquid nitrogen and stirs, be then put into carrier inner core in the outer tube (9) having been loaded with liquid nitrogen, and tighten, is put into freezing storing box
Medium-term and long-term preservation；
Vitrifying is recovered：Carrier is taken out from freeze box, outwards winding carrier inner core on liquid nitrogen surface, holding Handheld Division (1) will
The ovary tissue of tiling is moved into 37 DEG C of warmed-up resuscitation fluid I and balanced 1 minute, then ovary tissue is moved into resuscitation fluid II
Row equilibrium at room temperature 5 minutes, finally move in G-mops treatment fluids to balance 10 minutes and complete vitrifying recovery, can then carry out shape
Hormone determination after state is analyzed and cultivated.
Wherein, equilibrium liquid component is：The ethylene glycol of the dimethyl sulfoxide (DMSO) of basal liquid+7.5%+7.5%；
Glass freezing liquid component is：Ethylene glycol+0.5M the sucrose of the dimethyl sulfoxide (DMSO) of basal liquid+20%+20%；
Resuscitation fluid I components are：Basal liquid+1M sucrose；
Resuscitation fluid II components are：Basal liquid+0.5M sucrose；
Above-mentioned basal liquid component is the human serum albumins of G-mops treatment fluids+5% (HSA)；
Explanation is further explained to the present invention by the following examples, but is not construed as limiting the invention.
Embodiment
Referring to Fig. 1-4, the present invention provides a kind of bulk Vitrification of Ovarian Tissue carrier, including carrier inner core and
Outer tube.Carrier inner core is made up of the support sector (4) of Handheld Division (1), cap portion (2) and clamping metallic sieve.Inner core is provided with outer
Screw thread (5), outer tube are provided with internal thread (8), good sealing effect, avoid the cross-infection of ovary tissue.Wherein carrier inner core is each
Part is integrally formed, and bulk ovary tissue is located at the ovary tissue loading area (7) of metallic sieve contained by carrier inner core.
Metallic sieve of the present invention selects the stainless steel metal screen cloth of 80-120 mesh 304, has both met cryoprotector to tissue
Infiltration demand, take into account the soft durometer of freezing carrier again, facilitate ovary tissue Cryopreservation operate, also with it is cheap, do not give birth to
The advantages such as rust.Ovary tissue blots ovary tissue surface liquid, so before input liquid nitrogen carries out glass freezing with sterile gauze
Ovary tissue is laid on metallic sieve afterwards, being capable of fast cooling after ensureing into liquid nitrogen.
The present invention replaces traditional invaginating freezing pipe cap using Handheld Division, and the length of Handheld Division (1) is 1-2cm, facilitates ovum
Nest tissue loading operation, avoid tweezers from accommodating the drawbacks of unstable, will easily can directly be carried above liquid nitrogen surface by holding
Internal core is put into outer tube and tightened.
The present invention also provides a kind of method of bulk ovary tissue freezen protective, this method use above freezing carrier, obtains
Obtain part of test results and see Fig. 5 and Fig. 6.Experimenter is understood that the bulk ovary tissue glass that the invention provides by the example
The using effect of glass freezing carrier.
Glass freezing reagent：
Basal liquid：G-mops+5%HSA (G-mops is purchased from Vitrolife companies)
Equilibrium liquid：The ethylene glycol of the dimethyl sulfoxide (DMSO) of basal liquid+7.5%+7.5%
Glass freezing liquid：Ethylene glycol+0.5M the sucrose of the dimethyl sulfoxide (DMSO) of basal liquid+20%+20%
Resuscitation fluid I：Basal liquid+1M sucrose
Resuscitation fluid II：Basal liquid+0.5M sucrose
Operating procedure：
Glass freezing：Ovary tissue is cut into 1mm × (0.5-1) cm × (0.8-1.5) cm size tissue blocks, in equilibrium liquid
Middle equilibrium at room temperature 25min, the 15min in glass freezing liquid.After blotting ovary tissue surface liquid, (1) is held by ovary group
Knit and be laid in tissue loading area (7), cap portion (2) are put into liquid nitrogen with lower portion rapidly after loading and stirred, carrier inner core is filled
Enter in the outer tube (9) in liquid nitrogen, and tighten, be put into freezing storing box and preserve for a long time.
Vitrifying is recovered：Clamped with tweezers cryovial, outwarded winding carrier inner core with hand on liquid nitrogen surface, hold (1) by ovary
Tissue, which is moved into 37 DEG C of resuscitation fluid I of preheating, balances 1min, then ovary tissue is moved into resuscitation fluid II, equilibrium at room temperature 5min,
Move in G-mops after balancing 10min, carry out hormone determination after morphological analysis and culture.
Fig. 5 is using morphologic observation figure, wherein control group after present invention progress bulk Vitrification of Ovarian Tissue recovery
(Control) it is fresh ovarian tissues, experimental group (Vitrification) is ovary tissue after glass freezing recovery, it is seen that
Ovary tissue primordial follicle basement membrane after Cryopreservation is complete, and egg mother cell nuclear membrane is clear, and granular cell is uniform, and form is just
Often, there was no significant difference with fresh ovarian tissues；
Fig. 6 is that hormonal readiness determines after carrying out bulk Vitrification of Ovarian Tissue recovery using the present invention, wherein compareing
Group (Control) is fresh ovarian tissues hormone secretion level, and experimental group (Vitrification) is after glass freezing is recovered
Ovary tissue hormone secretion level, there was no significant difference with fresh ovarian tissues for its estradiol (E2) and progesterone (P) secretion level.
The preferred embodiment of the application is the foregoing is only, is not limited to the application, for the skill of this area
For art personnel, the application can have various modifications and variations.It is all within spirit herein and principle, made any repair
Change, equivalent substitution, improvement etc., should be included within the protection domain of the application.
Claims (10)
1. a kind of bulk Vitrification of Ovarian Tissue carrier, it is characterised in that the carrier is by carrier inner core and outer tube group
Into the carrier inner core is threadedly coupled with outer tube；
The carrier inner core is from top to bottom made up of Handheld Division, cap portion, support sector and supporting part successively, and the supporting part is by supporting
Portion grips；In use, bulk ovary tissue is disposed away from an end of the supporting part of support sector, the end is bulk
Ovary tissue loading area.
2. a kind of carrier as claimed in claim 1, it is characterised in that the supporting part is metallic sieve, it is preferred that the gold
Category screen cloth is the stainless steel metal screen cloth of 80-120 mesh 304.
3. a kind of carrier as claimed in claim 1, it is characterised in that the carrier inner core is provided with close with outer tube junction
Seal.
4. a kind of carrier as claimed in claim 1, it is characterised in that the Handheld Division diameter is less than cap portion diameter, it is preferred that
The Handheld Division is provided with multiple anti-skid bulges.
A kind of 5. carrier as claimed in claim 1, it is characterised in that support sector's external diameter is less than the internal diameter of outer tube, and
Carrier inner core is threadedly coupled by the external screw thread set in support sector with the internal thread set on outer tube with outer tube.
6. a kind of carrier as claimed in claim 1, it is characterised in that it is stable that the outer sleeve bottom is provided with one or more
Frame.
7. application of any one of the claim 1-6 carriers in Vitrification of Ovarian Tissue and vitrifying recovery.
8. application as claimed in claim 7, it is characterised in that the ovary tissue is bulk ovary tissue, it is preferred that described big
Block ovary tissue volume is 1mm × (0.5-1) cm × (0.8-1.5) cm.
9. as described in claim 7 or 8 application, it is characterised in that it is described application specific method be：
Glass freezing concrete application method is：By the first equilibrium at room temperature in equilibrium liquid of bulk ovary tissue, glass is subsequently placed in
Change freezing liquid in freezing processing for a period of time；After ovary tissue surface liquid is blotted, hold Handheld Division and put down bulk ovary tissue
Supporting part tissue loading area is layered on, carrier cap portion is placed in liquid nitrogen with lower portion rapidly after loading and stirred, then by carrier
Core is put into the outer tube having been loaded with liquid nitrogen, and is tightened, and is put into freezing storing box and is preserved for a long time；
Vitrifying recovery concrete application method is：Carrier is taken out from freeze box, carrier inner core is outwarded winding on liquid nitrogen surface, holds hand
Holding portion will be balanced in the warmed-up resuscitation fluid I of the ovary tissue immigration of tiling, then ovary tissue is moved into resuscitation fluid II and carried out
Equilibrium at room temperature, finally move to balance in G-mops treatment fluids and complete vitrifying recovery, can then carry out morphological analysis and culture
Hormone determination afterwards.
10. application as claimed in claim 9, it is characterised in that
The equilibrium liquid component is：The ethylene glycol of the dimethyl sulfoxide (DMSO) of basal liquid+7.5%+7.5%；
The glass freezing liquid component is：Ethylene glycol+0.5M the sucrose of the dimethyl sulfoxide (DMSO) of basal liquid+20%+20%；
The resuscitation fluid I components are：Basal liquid+1M sucrose；
The resuscitation fluid II components are：Basal liquid+0.5M sucrose；
The basal liquid component is the human serum albumins of G-mops treatment fluids+5%.
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| CN108641999A (en) * | 2018-03-26 | 2018-10-12 | 阮祥燕 | A kind of ovary tissue freeze and method for resuscitation |
| CN111700061A (en) * | 2020-06-12 | 2020-09-25 | 山东大学齐鲁医院 | Closed vitrification carrier and application thereof |
| CN111770682A (en) * | 2018-02-05 | 2020-10-13 | 尼特尔利泽尔有限责任公司 | Device for freezing adipose tissue |
| CN111838131A (en) * | 2020-07-16 | 2020-10-30 | 浙江大学 | Method for improving vitrification freezing efficiency of ovarian tissue |
| CN113016776A (en) * | 2019-12-24 | 2021-06-25 | 上海明悦医疗科技有限公司 | Carrier, vacuumizing device and tissue cryopreservation system |
| CN114403126A (en) * | 2021-12-06 | 2022-04-29 | 起源细胞技术(滁州)有限公司 | Closed embryo freezing tube and using method thereof |
| CN114514924A (en) * | 2021-03-23 | 2022-05-20 | 上海慧存医疗科技有限公司 | Ovarian tissue preservation method |
| CN114521550A (en) * | 2021-03-23 | 2022-05-24 | 上海慧存医疗科技有限公司 | Biological preservation solution and application thereof in oocyte and ovary tissue preservation |
| CN115399311A (en) * | 2021-05-26 | 2022-11-29 | 北京大学深圳医院 | Vitrification refrigerating fluid set and preparation method and application thereof |
| CN115843781A (en) * | 2022-12-16 | 2023-03-28 | 江西省妇幼保健院 | Carrier for ovarian tissue slice vitrification |
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