CN207305900U - A kind of bulk Vitrification of Ovarian Tissue carrier - Google Patents
A kind of bulk Vitrification of Ovarian Tissue carrier Download PDFInfo
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- CN207305900U CN207305900U CN201721307147.0U CN201721307147U CN207305900U CN 207305900 U CN207305900 U CN 207305900U CN 201721307147 U CN201721307147 U CN 201721307147U CN 207305900 U CN207305900 U CN 207305900U
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Abstract
The utility model discloses a kind of bulk Vitrification of Ovarian Tissue carrier is provided, the carrier is made of carrier inner core and outer tube, and the carrier inner core is threadedly coupled with outer tube, has both kept good leakproofness, prevents the cross-infection of ovary tissue；User's operation is also convenient at the same time；The carrier inner core is from top to bottom made of Handheld Division, cap portion, support sector and supporting part successively, and the supporting part is gripped by support sector；In use, bulk ovary tissue is disposed away from an end of the supporting part of support sector, the end, that is, bulk ovary tissue loading area；Experiments verify that using carrier described in the utility model, refrigerant osmotic efficiency, but also bulk storage easy to operate, cheap, easy to bulk ovary tissue are not only improved, suitable for promoting and applying.
Description
Technical field
The utility model belongs to medical instruments field, and in particular to a kind of bulk Vitrification of Ovarian Tissue carrier.
Background technology
Ovary tissue freezen protective is most fast and most effective preservation fertility for the adult female for treating chemicotherapy treatment
Method, for prepuberal women, ovary tissue freezing is to preserve the unique channel of fecundity.Traditional ovary group
Knitting Refrigeration Technique takes sequencing to freeze, and has helped several women to obtain the child of oneself by the technology at present.But program
Change Refrigeration Technique because the shortcomings of instrument price is expensive, cooling time is long, cumbersome limits the clinical expansion of ovarian freezing technology.
Vitrification is the trend of following cell/tissue cryogenic freezing, possesses that easy to operate, of low cost, freezing efficiency is high
Etc. advantage, sequencing freezing substantially completely instead of in human oocyte and Embryo freezing preservation field at present.Vitrifying
Refrigeration Technique is to utilize cell/tissue being put into special freezing carrier, and direct plunge into Liquid Nitrogen, makes cryoprotector moment by liquid
It is changed into hyaloid solid-state, avoids forming ice crystal into the cell.
Although glass freezing gradually starts to be applied to ovary tissue freezing region, freezing tissue volume maintains mostly
In 1mm × (1-5) mm × (1-5) mm, its main cause is that tissue block crosses conference and causes the cryoprotector short time not to be impregnated with
Middle section, and the exposure of long-time refrigerant can cause serious cytotoxic damage.Fritter ovary tissue (≤50mm3) due to
Only contain a small amount of ovarian follicle in transplanting tissue, endocrine function is held time shorter (1-3) after transplanting.And bulk ovary cortex
(≥80mm3) because containing abundant primordial follicle stock, hormone can be made to hold time longer, preserve and lead in female fertility
Domain has broad application prospects.Therefore, ovary middle section is protected during how realizing bulk Vitrification of Ovarian Tissue
The fully penetrated for protecting agent is successful key.
Freezing carrier is the device of vitrifying carrying cell/tissue, is one of successful key factor of glass freezing.Mesh
The vitrified frozen vector that preceding ovarian freezing uses mainly includes freezing straw, freezing ring, cell screen clothes, aculeus type carrier etc.,
Wherein freeze straw and freeze the ovary tissue that ring is only capable of loading smaller size smaller, aculeus type carrier can cause ovary tissue irreversible
Mechanical damage, occupancy volume is excessive when cell screen clothes are put into liquid nitrogen container storage, is not easy bulk storage.Therefore, above-mentioned carrier is in ovum
The clinical practice of nest tissue freezen protective faces many difficulties in promoting, and is not yet widely accepted.Current ovary tissue on the market
The Ova Cryo Device Type M carriers of the rarely seen KITAZATO companies of carrier, not only expensive (about 300 yuan /), and
It is easy to fall off and draw point and cortex contact area are small in ovary tissue loading process, it is difficult to popularize in clinical and research work.
Utility model content
For the deficiency of the design of the above-mentioned Vitrification of Ovarian Tissue carrier of bulk in the prior art, utility model people warp
Long-term technology and practical exploration, there is provided a kind of simple efficient, cheap bulk study on vitrification of ovary carrier.Through experiment
Verification, using carrier described in the utility model, not only improves refrigerant osmotic efficiency, and it is easy to operate, cheap, be easy to
The bulk storage of bulk ovary tissue, suitable for promoting and applying.
Specifically, it the utility model is related to following technical scheme：
The first aspect of the utility model, there is provided a kind of bulk Vitrification of Ovarian Tissue carrier, the carrier
It is made of carrier inner core and outer tube, the carrier inner core is threadedly coupled with outer tube, has both been kept good leakproofness, has been prevented ovum
The cross-infection of nest tissue；User's operation is also convenient at the same time；
The carrier inner core is from top to bottom made of Handheld Division, cap portion, support sector and supporting part successively, the supporting part by
Support sector grips；In use, bulk ovary tissue is disposed away from an end of the supporting part of support sector, the end is
Bulk ovary tissue loading area；
Preferably, the supporting part is metallic sieve, it is further preferred that the metallic sieve for 80-120 mesh 304 not
Become rusty steel metal sieve, and the utility model especially selects the stainless steel metallic sieve of specific mesh number as supporting part, one side
It disclosure satisfy that infiltration demand of the cryoprotector to ovary tissue, while take into account the hardness of ovarian freezing carrier, facilitate ovary
Cryopreservation operation is organized, while it also has the advantage such as cheap, non-corrosive, sturdy and durable；
Preferably, the carrier inner core is provided with sealing ring with outer tube junction, so as to strengthen sealing function, avoids ovum
The cross-infection of nest tissue；
Preferably, the Handheld Division diameter is less than cap portion diameter, convenient for users to taking, it is further preferred that the hand
The portion of holding is provided with multiple anti-skid bulges, and the user that is more convenient for takes；
Preferably, support sector's outside diameter is less than the internal diameter of outer tube, and carrier inner core in support sector by setting
External screw thread is threadedly coupled with the internal thread set on outer tube with outer tube；
Preferably, the outer sleeve bottom is provided with one or more steady rest, carrier inclined when preventing preservation.
Second aspect of the utility model, discloses the carrier and recovers in Vitrification of Ovarian Tissue and vitrifying
In application.
Specifically, the ovary tissue is bulk ovary tissue, it is preferred that the bulk ovary tissue volume for 1mm ×
(0.5-1)cm×(0.8-1.5)cm；The utility model is by well-designed, so as to improve Vitrification of Ovarian Tissue guarantor
Agent permeability is protected, glass freezing efficiency is improved, so as to fulfill the glass freezing to bulk ovary tissue.
It is described application specific method be：
Glass freezing：The bulk ovary tissue first equilibrium at room temperature in equilibrium liquid is subsequently placed in glass freezing liquid
Freezing processing is for a period of time；After ovary tissue surface liquid is blotted, hold Handheld Division and bulk ovary tissue is laid in supporting part
Loading area is organized, carrier cap portion is placed in liquid nitrogen with lower portion rapidly after loading and is stirred, then carrier inner core is put into and has been filled
Have in the outer tube in liquid nitrogen, and tighten, be put into freezing storing box and preserve for a long time；
Vitrifying is recovered：Carrier is taken out from freeze box, unscrews carrier inner core on liquid nitrogen surface, holding Handheld Division will tile
Ovary tissue move into warmed-up resuscitation fluid I and balance, then ovary tissue is moved into resuscitation fluid II and carries out equilibrium at room temperature, most
After move in G-mops treatment fluids balance and complete vitrifying recovery, hormone determination after can then carrying out morphological analysis and cultivating.
Wherein, equilibrium liquid component is：+ 7.5% ethylene glycol of the dimethyl sulfoxide (DMSO) of basal liquid+7.5%；
Glass freezing liquid component is：+ 20% ethylene glycol+0.5M sucrose of the dimethyl sulfoxide (DMSO) of basal liquid+20%；
Resuscitation fluid I components are：Basal liquid+1M sucrose；
Resuscitation fluid II components are：Basal liquid+0.5M sucrose；
Above-mentioned basal liquid component is+5% human serum albumins of G-mops treatment fluids (HSA)；
Compared with prior art, the utility model has the beneficial effect that：
(1) cost is low, easy to operate, bulk Vitrification of Ovarian Tissue carrier the selection of material prepared by the utility model
Source is wide, price is low；Operating process can operate at room temperature, avoid the great number expense that Slow-rate freezing instrument is used in traditional freezing
With and complicated procedures；
(2) carrier outer tube is mounted with liquid nitrogen in the utility model so that liquid nitrogen and the ovary being fixed on metallic sieve
Tissue directly contacts, and metallic sieve has good heat conductivility, so as to accelerate chilling rate, extends cooling time, and subtract
The cooling time of few sample；At the same time, it is necessary to which explanation, liquid nitrogen is placed in outer tube in the utility model, when carrier inner core with
After outer tube is connected through a screw thread closure, i.e., forms autonomous closure with ovary tissue and contact, avoid ovary tissue from being subject to potential
Other pathogenic microorganisms, virus pollution, and during closure progress freezen protective, the metallic sieve after screwing maintains stationary state,
Ovary tissue sample is prevented because rocking disengaging metal mesh, so that more convenient ovary tissue sample access；
(3) carrier operation described in the utility model is used, the cryoprotector volume of ovary tissue surface attachment can be dropped
It is extremely minimum, so as to effectively improve rate of temperature fall and reduce the remaining adverse effect of glass freezing liquid, simultaneously as vitrifying is cold
Freeze rate of temperature fall to significantly improve, therefore the glass freezing liquid of low concentration can be used to be carried out dehydrating, therefore into one
Step reduces glass freezing liquid risk of toxicity；
(4) carrier described in the utility model and application method are used, can well preserve the form and vigor of ovarian follicle, at the same time
The carrier of the utility model design can be with the relatively large ovary tissue of directly freezed, so as to further increase ovary tissue survival
Rate；
(5) the utility model design vector reduces ovary tissue on the basis of not influencing or even increasing ease of use
The shared space of freezing, is greatly saved floor space needed for stored frozen, easy to bulk storage, suitable for ovary tissue storehouse
Foundation and clinical application.
Brief description of the drawings
Fig. 1 is freezing carrier model core arrangement schematic diagram in the utility model, and wherein Fig. 1 (a) is front view, and Fig. 1 (b) is side
View；
Fig. 2 is freezing carrier inner core bottom view in the utility model；
Fig. 3 is freezing carrier outer tube profile in the utility model；
Fig. 4 is freezing carrier outer tube bottom view in the utility model；
Fig. 5 is using morphologic observation figure after the utility model progress bulk Vitrification of Ovarian Tissue recovery；
Fig. 6 is that hormonal readiness measures after carrying out bulk Vitrification of Ovarian Tissue recovery using the utility model.
Wherein, 1- Handheld Divisions, 2- caps portion, 3- sealing rings, 4- support sectors, 5- external screw threads, 6- supporting parts, 7- ovary tissues dress
Carry area, 8- internal threads, 9- outer tubes, 10- steady rests.
Embodiment
It is noted that described further below is all illustrative, it is intended to provides further instruction to the application.It is unless another
Indicate, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singulative
It is also intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " bag
Include " when, it indicates existing characteristics, step, operation, device, component and/or combinations thereof.
In the utility model, term as " on ", " under ", "left", "right", "front", "rear", " vertical ", " level ",
The orientation or position relationship of the instructions such as " side ", " bottom " are based on orientation shown in the drawings or position relationship, only to facilitate chatting
State each component of the utility model or component structure relation and definite relative, not refer in particular in the utility model either component or
Element, it is impossible to be interpreted as the limitation to the utility model.
As background technology is introduced, the vitrified frozen vector used in the prior art ovarian freezing mainly includes
Straw, freezing ring, cell screen clothes, aculeus type carrier are freezed, but generally existing freezing ovary tissue is too small, and refrigerating effect is bad,
There is damage to ovary tissue, the various problems such as price is high；
In view of this, in an exemplary embodiment of the utility model, there is provided a kind of bulk ovary tissue vitrifying is cold
Freeze carrier, the carrier is made of carrier inner core and outer tube (9), and the carrier inner core is threadedly coupled with outer tube (9)；
The carrier inner core is from top to bottom made of Handheld Division (1), cap portion (2), support sector (4) and supporting part (6) successively,
The supporting part (6) is gripped by support sector (4)；In use, bulk ovary tissue is disposed away from the supporting part of support sector (4)
(6) a end, the end, that is, bulk ovary tissue loading area (7)；
Wherein, the supporting part (6) is metallic sieve, it is further preferred that the metallic sieve for 80-120 mesh 304 not
Become rusty steel metal sieve；
The carrier inner core is provided with sealing ring (3) with outer tube (9) junction, so as to strengthen sealing function, avoids ovum
The cross-infection of nest tissue；
Handheld Division (1) diameter is less than cap portion (2) diameter, and the Handheld Division (1) is provided with multiple anti-skid bulges, more just
Taken in user；
Support sector (4) outside diameter is less than the internal diameter of outer tube (9), and carrier inner core is by being set in support sector (4)
External screw thread (5) be threadedly coupled with the internal thread (8) set on outer tube (9) with outer tube；
The outer sleeve bottom is provided with one or more steady rest (10), carrier inclined when preventing preservation.
In the still another embodiment of the utility model, the carrier is disclosed in Vitrification of Ovarian Tissue and glass
Application in glassization recovery.
Specifically, the ovary tissue is bulk ovary tissue, the bulk ovary tissue volume is 1mm × (0.5-1)
cm×(0.8-1.5)cm；
It is described application specific method be：
Glass freezing：Ovary tissue is cut into 1mm × (0.5-1) cm × (0.8-1.5) cm size tissue blocks, in equilibrium liquid
Middle equilibrium at room temperature 25min, the 15min in glass freezing liquid；After ovary tissue surface liquid is blotted, holding Handheld Division (1) will
Bulk ovary tissue is laid in supporting part (6) ovary tissue loading area (7), rapidly by carrier cap portion (1) with lower portion after loading
It is placed in liquid nitrogen and stirs, be then put into carrier inner core in the outer tube (9) having been loaded with liquid nitrogen, and tighten, is put into freezing storing box
Medium-term and long-term preservation；
Vitrifying is recovered：Carrier is taken out from freeze box, unscrews carrier inner core on liquid nitrogen surface, holding Handheld Division (1) will
The ovary tissue of tiling is moved into 37 DEG C of warmed-up resuscitation fluid I and balance 1 minute, then by ovary tissue immigration resuscitation fluid II into
Row equilibrium at room temperature 5 minutes, finally moves in G-mops treatment fluids to balance 10 minutes and completes vitrifying recovery, can then carry out shape
Hormone determination after state is analyzed and cultivated.
Wherein, equilibrium liquid component is：+ 7.5% ethylene glycol of the dimethyl sulfoxide (DMSO) of basal liquid+7.5%；
Glass freezing liquid component is：+ 20% ethylene glycol+0.5M sucrose of the dimethyl sulfoxide (DMSO) of basal liquid+20%；
Resuscitation fluid I components are：Basal liquid+1M sucrose；
Resuscitation fluid II components are：Basal liquid+0.5M sucrose；
Above-mentioned basal liquid component is+5% human serum albumins of G-mops treatment fluids (HSA)；
Explanation is further explained to the utility model by the following examples, but does not form the limit to the utility model
System.
Embodiment
Referring to Fig. 1-4, the utility model provides a kind of bulk Vitrification of Ovarian Tissue carrier, including in carrier
Core and outer tube.Carrier inner core is made of the support sector (4) of Handheld Division (1), cap portion (2) and clamping metallic sieve.Inner core is set
There is external screw thread (5), outer tube is equipped with internal thread (8), and sealing effect is good, avoids the cross-infection of ovary tissue.Wherein in carrier
Each component of core is integrally formed, and bulk ovary tissue is located at the ovary tissue loading area (7) of metallic sieve contained by carrier inner core.
The utility model metallic sieve selects 304 stainless steel metal sieve of 80-120 mesh, has both met cryoprotector to group
The infiltration demand knitted, and take into account the hardness of freezing carrier, facilitates ovary tissue Cryopreservation to operate, also with it is cheap,
The advantages such as non-corrosive.Ovary tissue blots ovary tissue surface liquid before input liquid nitrogen carries out glass freezing with sterile gauze
Ovary tissue, is then laid on metallic sieve by body, being capable of fast cooling after ensureing into liquid nitrogen.
The utility model replaces traditional invaginating to freeze pipe cap using Handheld Division, and the length of Handheld Division (1) is 1-2cm, side
Just ovary tissue loading operation, avoids tweezers from accommodating the drawbacks of unstable, can be easily above liquid nitrogen surface directly by holding
Carrier inner core is put into outer tube and is tightened.
The utility model also provides a kind of method of bulk ovary tissue freezen protective, more than this method use freezes and carries
Body, obtains part of test results and sees Fig. 5 and Fig. 6.Experimenter is understood that the bulk ovum that the utility model provides by the example
The using effect of nest tissue vitrified frozen vector.
Glass freezing reagent：
Basal liquid：G-mops+5%HSA (G-mops is purchased from Vitrolife companies)
Equilibrium liquid：+ 7.5% ethylene glycol of the dimethyl sulfoxide (DMSO) of basal liquid+7.5%
Glass freezing liquid：+ 20% ethylene glycol+0.5M sucrose of the dimethyl sulfoxide (DMSO) of basal liquid+20%
Resuscitation fluid I：Basal liquid+1M sucrose
Resuscitation fluid II：Basal liquid+0.5M sucrose
Operating procedure：
Glass freezing：Ovary tissue is cut into 1mm × (0.5-1) cm × (0.8-1.5) cm size tissue blocks, in equilibrium liquid
Middle equilibrium at room temperature 25min, the 15min in glass freezing liquid.After blotting ovary tissue surface liquid, (1) is held by ovary group
Knit and be laid in tissue loading area (7), cap portion (2) are put into liquid nitrogen with lower portion rapidly after loading and are stirred, carrier inner core is filled
Enter in the outer tube (9) in liquid nitrogen, and tighten, be put into freezing storing box and preserve for a long time.
Vitrifying is recovered：Clamped with tweezers cryovial, unscrewed carrier inner core with hand on liquid nitrogen surface, hold (1) by ovary
Tissue, which is moved into 37 DEG C of resuscitation fluid I of preheating, balances 1min, then ovary tissue is moved into resuscitation fluid II, equilibrium at room temperature 5min,
Move in G-mops after balancing 10min, carry out hormone determination after morphological analysis and culture.
Fig. 5 is using morphologic observation figure after the utility model progress bulk Vitrification of Ovarian Tissue recovery, wherein right
It is fresh ovarian tissues according to group (Control), experimental group (Vitrification) is ovary tissue after glass freezing recovery,
It can be seen that the ovary tissue primordial follicle basement membrane after Cryopreservation is complete, egg mother cell nuclear membrane is clear, and granular cell is uniform, shape
State is normal, and there was no significant difference with fresh ovarian tissues；
Fig. 6 is that hormonal readiness measures after carrying out bulk Vitrification of Ovarian Tissue recovery using the utility model, wherein
Control group (Control) is fresh ovarian tissues hormone secretion level, and experimental group (Vitrification) is answered for glass freezing
Ovary tissue hormone secretion level after Soviet Union, its estradiol (E2) and progesterone (P) secretion level are with fresh ovarian tissues without conspicuousness
Difference.
The foregoing is merely the preferred embodiment of the application, the application is not limited to, for the skill of this area
For art personnel, the application can have various modifications and variations.It is all within spirit herein and principle, made any repair
Change, equivalent substitution, improvement etc., should be included within the protection domain of the application.
Claims (10)
1. a kind of bulk Vitrification of Ovarian Tissue carrier, it is characterised in that the carrier is by carrier inner core and outer tube group
Into the carrier inner core is threadedly coupled with outer tube；
The carrier inner core is from top to bottom made of Handheld Division, cap portion, support sector and supporting part successively, and the supporting part is by supporting
Portion grips.
2. a kind of bulk Vitrification of Ovarian Tissue carrier as claimed in claim 1, it is characterised in that the supporting part is remote
One end end from support sector is bulk ovary tissue loading area.
3. a kind of bulk Vitrification of Ovarian Tissue carrier as claimed in claim 1, it is characterised in that the supporting part is
Metallic sieve.
A kind of 4. bulk Vitrification of Ovarian Tissue carrier as claimed in claim 3, it is characterised in that the metallic sieve
For 304 stainless steel metal sieve of 80-120 mesh.
A kind of 5. bulk Vitrification of Ovarian Tissue carrier as claimed in claim 1, it is characterised in that the carrier inner core
Outer tube junction is provided with sealing ring.
6. a kind of bulk Vitrification of Ovarian Tissue carrier as claimed in claim 1, it is characterised in that the Handheld Division is straight
Footpath is less than cap portion diameter.
7. bulk Vitrification of Ovarian Tissue carrier as claimed in claim 1, it is characterised in that the Handheld Division is provided with
Multiple anti-skid bulges.
8. bulk Vitrification of Ovarian Tissue carrier as claimed in claim 1, it is characterised in that support sector's outside diameter is small
In the internal diameter of outer tube.
9. bulk Vitrification of Ovarian Tissue carrier as claimed in claim 1, it is characterised in that the carrier inner core passes through
The external screw thread set in support sector is threadedly coupled with the internal thread set on outer tube with outer tube.
10. bulk Vitrification of Ovarian Tissue carrier as claimed in claim 1, it is characterised in that the outer sleeve bottom
It is provided with one or more steady rest.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721307147.0U CN207305900U (en) | 2017-10-11 | 2017-10-11 | A kind of bulk Vitrification of Ovarian Tissue carrier |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721307147.0U CN207305900U (en) | 2017-10-11 | 2017-10-11 | A kind of bulk Vitrification of Ovarian Tissue carrier |
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| Publication Number | Publication Date |
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| CN207305900U true CN207305900U (en) | 2018-05-04 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110476952A (en) * | 2019-09-06 | 2019-11-22 | 苏州贝康医疗器械有限公司 | Vitrified frozen vector |
-
2017
- 2017-10-11 CN CN201721307147.0U patent/CN207305900U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110476952A (en) * | 2019-09-06 | 2019-11-22 | 苏州贝康医疗器械有限公司 | Vitrified frozen vector |
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