CN212325242U - Cell freezing auxiliary device - Google Patents
Cell freezing auxiliary device Download PDFInfo
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- CN212325242U CN212325242U CN202021464614.2U CN202021464614U CN212325242U CN 212325242 U CN212325242 U CN 212325242U CN 202021464614 U CN202021464614 U CN 202021464614U CN 212325242 U CN212325242 U CN 212325242U
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Abstract
The utility model belongs to the technical field of the biomaterial freezes, a cell freezing auxiliary device is disclosed. The utility model comprises a container vessel which is attached to the upper end of a carrying rod, wherein the middle part of the container vessel is provided with a through hole, and the lower end of the through hole corresponds to a biological material loading part of the carrying rod; the upper end of the container vessel is provided with a liquid outlet groove, a liquid discharge cavity is arranged inside the container vessel, and the liquid discharge cavity is respectively communicated with the liquid outlet groove and the through hole. The utility model is convenient for processing cells before freezing, is convenient for quickly and accurately placing the cells into the through hole, and is also convenient for the cells to accurately fall into the carrying rod in the later period, so that the freezing liquid wrapping the biological material has small volume and is stable, the operation is more convenient and faster, the cells do not need to be moved, and the operation steps are saved; the liquid outlet groove is connected with the through hole through the liquid outlet cavity, so that various vitrification solutions can be sucked away conveniently, the used vitrification solutions can be treated more easily and conveniently, the efficiency is higher, and the stability, timeliness and safety of operation are integrally guaranteed.
Description
Technical Field
The utility model belongs to the technical field of the biomaterial freezes, concretely relates to cell freezing auxiliary device.
Background
The art of vitrification cryopreservation of human and animal embryos is currently a relatively mature art, where "freezing" is liquid to solid cooling, which may include crystallization, and "vitrification" is liquid to solid cooling, but not crystallization. The vitrifying cryopreservation of human and animal embryos consists in the steps of collection and retrieval of the oocytes, their in vitro fertilization and subsequent storage of such fertilized eggs and the embryos and/or the resulting later blastocysts in an ultra-low temperature environment after treatment in a cryoprotective solution. The factors that influence the freezing process include osmotic shock and toxic shock of the freezing solution, knowledge and skill of the operator, and performance of the operating tools, such as a tool for freezing and loading cells, also known as a loading rod. The good carrier rod can ensure that the cells can obtain higher freezing and thawing speed and operability.
Carrier rods are available on the market as hollow tubes, or loop/hook type devices, such as Cryylogic, sold under the trade name fibreplug or Cryyloop, as defined in WO 00/21365. More typically other tools "Cryotop" as disclosed in international application WO 02/085110. Cryotop is an elastic strip of plastic joined to a grip. Wherein the cells were placed on a strip and then directly plunged into liquid nitrogen. At present, Cryotop is a mainstream technology with high survival rate and relative easy operability.
There are also many brand cryoprotectants on the market, with the principle being largely the same and different, in order to inhibit the formation of ice crystals within the cells and to minimize cell damage during the freezing process. These cryoprotectants are classified as osmotic and non-osmotic solutions. Examples of permeability are Ethylene Glycol (EG), dimethyl sulfoxide (DMSO), and glycerol. The osmotic cryoprotectant is a small molecule that readily penetrates the biofilm, forming hydrogen bonds with water molecules of the biomaterial, preventing its ice crystallization. Impermeable cryoprotectants, such as disaccharides, trehalose, and sucrose, function by extracting free water from within the biological material and dehydrating the intracellular space. The resulting dehydration allows it to complement the osmotic cryoprotectant to increase the intracellular relative concentration of the cryoprotectant, and to prevent the formation of ice crystals within the cell. However, the toxicity of these high concentrations of cryoprotectants can be considerable and the cells need to be rapidly plunged into liquid nitrogen after cryoprotectant pretreatment to achieve freezing. The very fast cooling rate minimizes the negative impact of the cryoprotectant on the biological material and also minimizes ice crystal formation due to imparting the desired vitrification.
The vitrification process involves exposing the biological material to at least three vitrification solutions. The vitrification solution is typically added to successive wells of a multi-well culture dish, wherein the culture dish and solution are warmed to a predetermined temperature, which is determined according to the requirements of the biological material under investigation.
In a typical protocol, the biological material is physically transferred to a first solution (e.g., ES equilibrator) in a first well, and then washed by physically moving the biological material or cells through the solution of interest using a cell pipetting device. The washing process is repeated in a second solution (e.g., VS vitrified cryo fluid), third and fourth wells in a second well for a predetermined period of time until the biological material or cells are considered ready for cryopreservation. The biological material is then physically aspirated with a predetermined amount of vitrification solution using a pipette or other manipulation device. The droplets containing the biological material or cells to be vitrified are then pipetted onto a vitrification device, such as a slide bar Cryotop. The vitrification device with the attached droplets and biological material is then physically transferred and directly plunged into liquid nitrogen. Once the biological material and carrier fluid are vitrified, the vitrification device is inserted into a pre-cooled protective sleeve or other storage device for subsequent transfer to liquid nitrogen or liquid nitrogen vapor for long-term cold storage.
Other tools disclosed in the prior art, "cryotops", are resilient strips that connect a piece of plastic. Wherein the sample is placed on a strip and then directly plunged into liquid nitrogen. The protocol is manually operated, and is a benchmark for germ cell vitrification freezing technology at present with mature technology and higher cell survival rate.
The cell freezing process of my prior art is basically performed manually. When people manually work, problems of mental stress, hand trembling, visual illusion, fatigue and the like can occur, and operation errors such as embryo or cell loss can occur, so that the stability, timeliness, safety and the like of operation cannot be guaranteed.
SUMMERY OF THE UTILITY MODEL
In order to solve the above problems existing in the prior art, the present invention is directed to a cell freezing assisting device.
The utility model discloses the technical scheme who adopts does:
a cell freezing auxiliary device comprises a carrying rod and a container vessel attached to the upper end of the carrying rod, wherein a through hole is formed in the middle of the container vessel, and the lower end of the through hole corresponds to a biological material loading part of the carrying rod; the upper end of the container is provided with a liquid outlet groove, the interior of the container is provided with a liquid outlet cavity, and the liquid outlet cavity is respectively communicated with the liquid outlet groove and the through hole.
Preferably, one end of the liquid discharge cavity is communicated with the lower part of the liquid outlet groove, the other end of the liquid discharge cavity surrounds the periphery of the through hole, a plurality of liquid discharge channels are uniformly distributed between the through hole and the liquid discharge cavity, and each liquid discharge channel is communicated with the through hole and the liquid discharge cavity.
It is further preferred that each drainage channel is tangential to the through-going hole.
Still further preferably, the vessel includes a transparent observation layer at the upper end of the liquid discharge cavity, and the through hole and the liquid discharge groove both penetrate through the transparent observation layer.
It is further preferable that the upper portion of the through hole corresponding to the transparent observation layer is a funnel-shaped structure with an opening diameter gradually decreasing from top to bottom.
More preferably, the container further comprises a solution treatment layer positioned at the lower end of the transparent observation layer and a release layer positioned at the lower end of the solution treatment layer, and the upper end of the carrying rod is attached to the lower end of the release layer; the liquid discharge cavity and all the liquid discharge channels are grooves arranged at the upper end of the solution treatment layer; the release layer is made of an elastic material.
It is further preferable that the solution treatment layer has a funnel-shaped structure in which the opening diameter of the through hole gradually decreases from top to bottom.
Still further preferably, the container is provided with a plurality of air holes, and the lower end of each air hole is correspondingly arranged on the carrying rod.
It is further preferred that the distance between each air hole and the central axis of the through-hole is gradually smaller from top to bottom.
It is further preferable that the lower end of the release layer is provided with an annular gas groove located outside the through hole, and the lower end of each gas hole is communicated with the annular gas groove.
The utility model has the advantages that:
the utility model is convenient for processing cells before freezing, increasing the container, arranging the through hole on the container, which can properly enlarge the size of the through hole, conveniently and rapidly and accurately placing the cells into the through hole, and also facilitating the cells to accurately fall onto the carrying rod in the later period, so that the operation is more convenient and rapid, the cells are not required to be moved, and the operation steps are saved; the liquid outlet groove is connected with the through hole through the liquid outlet cavity, so that various vitrification solutions can be sucked away conveniently, the used vitrification solutions can be treated more easily and conveniently, the efficiency is higher, and the stability, timeliness and safety of operation are integrally guaranteed.
Drawings
FIG. 1 is a schematic view of an explosion structure of the container of the present invention;
FIG. 2 is a schematic view of the three-dimensional structure of the container according to the present invention;
FIG. 3 is a cross-sectional view of the container of the present invention;
fig. 4 is a front view of the present invention.
In the figure: 1-a carrier bar; 2-container vessel; 201-transparent viewing layer; 202-solution treatment layer; 203-a release layer; 3-a through hole; 4-a liquid outlet groove; 5-a liquid discharge cavity; 6-a liquid drainage channel; 7-pores; 8-annular gas groove.
Detailed Description
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the present invention will be briefly described below with reference to the accompanying drawings and the description of the embodiments or the prior art, and it is obvious that the following description of the structure of the accompanying drawings is only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without any inventive work.
The technical solution provided by the present invention will be described in detail by way of embodiments with reference to the accompanying drawings. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto.
In some instances, some embodiments are not described or not in detail, as they are conventional or customary in the art.
Furthermore, the technical features described herein, or the steps of all methods or processes disclosed, may be combined in any suitable manner in one or more embodiments, in addition to the mutually exclusive features and/or steps. It will be readily appreciated by those of skill in the art that the order of the steps or operations of the methods associated with the embodiments provided herein may be varied. Any order in the drawings and examples is for illustrative purposes only and does not imply that a certain order is required unless explicitly stated to be required.
The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The terms "connected" and "coupled" when used in this application, encompass both direct and indirect connections (and couplings) where appropriate and where not necessary contradictory.
An object of the utility model is to provide a can be used for micromanipulation of biomaterial and container ware of storage, the lid carries the pole on CryoTop, carries to form the micro-container on the pole is carried to CryoTop, and the cell solution is handled the back, removes the container ware to save the step that the cell removed, including but not limited to the cultivation and the cryopreservation of these materials. Another object of the present invention is to facilitate the use of automated control of washing protocol times and reduce embryo handling, thereby achieving full automation. The biological material of the utility model can be fertilized ovum or oocyte.
The first embodiment is as follows:
as shown in fig. 1-4, the embodiment provides a cell freezing assisting device, which includes a carrying rod 1, and further includes a container 2 attached to an upper end of the carrying rod 1, wherein a through hole 3 is formed in a middle portion of the container 2, so that a cell and a vitrification solution, such as a culture medium, e.g., an ES solution, a VS solution, a cryopreservation medium, a thawing medium, a vitrification medium, a fertilization medium, or a buffer solution, can be put through the through hole, the through hole 3 has a lower end corresponding to a biomaterial loading portion of the carrying rod 1, and a lower portion of the through hole 3 is mainly used for passing through the cell, so that a lower dimension of the through hole 3 can be designed according to a size of the cell, and dimensions of an upper portion and a middle portion of the through hole 3 are not specifically limited, so as to facilitate passing; hold the upper end of household utensils 2 and be provided with out liquid tank 4, imbibition device, for example imbibition pump, can be connected to the upper end of out liquid tank 4, and the inside of container household utensils 2 is provided with flowing back chamber 5, and flowing back chamber 5 communicates out the lower extreme of liquid tank 4 and the middle part of through hole 3 respectively. It should be noted that, in the using process, a vitrification solution or a vitrification solution with cells is added from the through hole, when the vitrification solution needs to be replaced, the vitrification solution in the through hole 3 is sucked by the liquid outlet tank 4 through the liquid outlet cavity 5, and then other vitrification solutions are replaced, after the cell culture is completed, the cells fall onto the carrying rod 1 from the through hole, and the container dish 2 is separated from the carrying rod, so that the carrying rod with cells can conveniently perform the cell freezing operation. It is further noted that the vessel 2 preferably comprises a cap or lid adapted to seal the opening, either as a separate lid or an integrally attached lid. It should be noted that, in the actual operation process, in order to enhance the sealing effect between the carrying rod and the container dish, the article such as tweezers may be used to enhance the adhesion between the carrying rod and the container dish, or other articles may be used to compress the carrying rod and the container dish, so as to avoid a gap between the carrying rod and the container dish, so that the cells are left on the carrying rod, and the carrying rod is conveniently thrown into liquid nitrogen in the subsequent process.
The utility model discloses be convenient for to the processing of cell before the cell freezes, increase and hold household utensils 2, set up through hole 3 on the container article, can suitably enlarge the size of through hole like this, be convenient for put into the through hole fast accurately, also be convenient for later stage cell accurate fall into on carrying the pole, make the operation convenient and fast more, also need not to remove the cell again, saved the operating procedure; the liquid outlet groove 4 is connected with the through hole 3 through the liquid outlet cavity 5, so that various vitrification solutions can be sucked away conveniently, the used vitrification solutions can be treated more easily and conveniently, the step of cell movement is omitted, the efficiency is higher, and the stability, timeliness and safety of operation are integrally guaranteed.
Example two:
the present embodiment is a further improvement on the basis of the first embodiment, and specific differences between the present embodiment and the first embodiment are as follows:
it should be further explained in this embodiment that one end of the liquid discharge cavity 5 is communicated with the lower part of the liquid outlet tank 4, so that liquid can flow between the liquid discharge cavity 5 and the liquid outlet tank 4, and later-stage liquid discharge is facilitated, the other end of the liquid discharge cavity 5 surrounds the through hole 3, a plurality of liquid discharge channels 6 are uniformly distributed between the through hole 3 and the liquid discharge cavity 5, and each liquid discharge channel 6 is communicated with the through hole 3 and the liquid discharge cavity 5. In practice, the aperture of the drainage channel 6 is small, so that cells can be prevented from passing through the drainage channel 6, the cells can be limited in the through hole, and the stability of operation is ensured.
It should be further noted that each drainage channel 6 is tangent to the through hole 3, so as to form a similar vortex-like structure, which can properly accelerate the liquid circulation and improve the working efficiency.
Example three:
the present embodiment is a further improvement on the basis of the second embodiment, and specific differences between the present embodiment and the second embodiment are:
in this embodiment, it should be further noted that the container 2 includes a transparent observation layer 201 at the upper end of the liquid discharge cavity 5, and both the through hole 3 and the liquid outlet groove 4 penetrate through the transparent observation layer 201. It should be noted that the transparent viewing layer 201 can be formed of, but not limited to, a transparent medical material, such as PDMS or PMMA, for facilitating microscopic viewing.
Example four:
the present embodiment is a further improvement on the basis of the third embodiment, and specific differences between the present embodiment and the third embodiment are as follows:
it should be further described that the upper portion of the through hole 3 corresponding to the transparent observation layer 201 is a funnel-shaped structure with an opening diameter gradually decreasing from top to bottom, and the opening is a little larger, so that cells and vitrification solution can be conveniently and rapidly placed into the through hole 3, and the operation is easier and more convenient.
Example five:
the present embodiment is a further improvement on the basis of the fourth embodiment, and specific differences between the present embodiment and the fourth embodiment are:
in this embodiment, it should be further described that the container 2 further includes a solution treatment layer 202 located at the lower end of the transparent observation layer 201 and a release layer 203 located at the lower end of the solution treatment layer 202, the upper end of the carrying rod 1 is attached to the lower end of the release layer 203, specifically, the biomaterial loading portion of the carrying rod 1 corresponds to the through hole corresponding to the release layer 203, so as to ensure that the cells can accurately fall on the carrying rod; the liquid discharge cavity 5 and all the liquid discharge channels 6 are grooves arranged at the upper end of the solution treatment layer 202, and then a cavity is formed by matching and sealing the transparent observation layer 201, so that the conditions of the liquid discharge cavity 5 and all the liquid discharge channels 6 can be observed through the transparent observation layer 201, and the observation is clearer; the release layer 203 is made of an elastic material, such as a PDMS material or a PMMA material, is not particularly limited, has a certain elasticity, can enhance the tightness between the lower end of the container and the carrier rod, and has a sealing effect similar to an O-ring.
Example six:
the present embodiment is a further improvement made on the basis of the fifth embodiment, and the specific differences between the present embodiment and the fifth embodiment are:
it should be further explained in this embodiment that the middle of the through hole 3 corresponding to the solution processing layer 202 is a funnel-shaped structure with an opening diameter gradually decreasing from top to bottom. It should be noted that the aperture of the through hole 3 corresponding to the solution treatment layer 202 is relatively small, which facilitates the cells to fall into it, and meanwhile, it is not too large, which prevents too much vitrification solution from existing inside, and ensures high efficiency of liquid outlet through the liquid outlet tank 4.
Example seven:
the present embodiment is a further improvement made on the basis of any one of the first to fifth embodiments, and the specific differences between the present embodiment and the fifth embodiment are:
it should be further explained in this embodiment that the container 2 is provided with a plurality of air holes 7, the lower end of each air hole 7 is correspondingly arranged on the carrying rod 1, so that the container 2 is required to be separated from the carrying rod, but a certain suction force exists between the two, so that the two are not well separated, and the container 2 can be easily separated from the carrying rod by inflating the air holes 7. It should be noted that the arrangement form of the air holes is not specifically limited, and may be a vertical hole, an inclined hole, or a stepped hole with a gradually changing central axis position. Further, when the vessel 2 includes a three-layer structure of the transparent observation layer 201, the solution treatment layer 202, and the release layer 203, which are arranged in this order from the top to the bottom, each of the air holes is formed through the three-layer structure.
Example eight:
the present embodiment is a further improvement on the basis of the seventh embodiment, and specific differences between the present embodiment and the seventh embodiment are:
what should be further described in the embodiment is that the distance between each air hole 7 and the central axis of the through hole 3 is gradually reduced from top to bottom, so that the outlet of each air hole can be maximally ensured to be arranged on the carrying rod, the condition that the outlet of each air hole is positioned outside the carrying rod can be avoided, and the air holes can be ensured to play a role when needed. It should be further detailed that each air hole can be divided into at least two sections of vertical air holes, and no two adjacent sections of vertical air holes can be connected by a strip hole, and the two adjacent vertical air holes are respectively communicated with two ends of the strip hole, so that the distance between the air hole 7 and the central axis of the through hole 3 is gradually reduced from top to bottom.
Example nine:
the present embodiment is a further improvement made on the basis of the seventh embodiment or the eighth embodiment, and the specific differences between the present embodiment and the seventh embodiment or the eighth embodiment are:
it should be further described in the embodiment that the lower end of the release layer 203 is provided with the annular air groove 8 located outside the through hole 3, and the lower end of each air hole 7 is communicated with the annular air groove 8, so that the outlet of the air hole 7 can be enlarged, and as long as the through hole is correspondingly arranged on the carrying rod, it can be ensured that at least one section of the annular air groove 8 is located on the carrying rod, and further, the air hole can blow air, so that the container 2 can achieve the effect of releasing from the carrying rod.
The vessel material is preferably selected from materials that can be sterilized and are biologically inert so as not to contaminate the biological material.
The utility model discloses can be used to manual operation, also can be used to the automation mechanized operation. In another aspect, the present invention provides an apparatus, which is an automated cryopreservation device, comprising a configuration for one or more of the vessels described previously, wherein the apparatus further comprises an element for washing captured biological material with vitrification and/or other processing solutions and an element for vitrifying captured biological material.
In another aspect, the present invention provides an automated microchannel cover useful for a semi-or automated process, wherein the apparatus comprises an element for washing captured biological material with a pre-vitrification or vitrification process solution and a jet to prepare the captured biological material for vitrification.
In another aspect, the present invention provides a method for cryopreservation of biological material, comprising the steps of:
placing the container vessel above the Cryotop, and compacting to enable the Cryotop and the container vessel to form a small container area;
introducing the biological material into a small container region of said container and flushing the biological material with a series of introductions and discharges from the vitrification solution;
the vessel is peeled away because the cell area is small enough to dispense a minimum of final vitrification solution to achieve vitrification of the container and captured biological material.
The series of vitrification solutions preferably includes increasing amounts of non-penetrating and penetrating cryoprotectants selected from the group consisting of Ethylene Glycol (EG), dimethyl sulfoxide (DMSO), glycerol, disaccharides, trehalose, and sucrose to enhance the settling of the biological material to the bottom of the sub-reservoir region to maximize drainage and minimize wash solution retention prior to vitrification or freezing.
The present invention is not limited to the above-mentioned optional embodiments, and any other products in various forms can be obtained by anyone under the teaching of the present invention, and any changes in the shape or structure thereof, all the technical solutions falling within the scope of the present invention, are within the protection scope of the present invention.
Claims (10)
1. A cell freezing assisting device comprises a carrying rod (1), and is characterized in that: the biological material loading device is characterized by further comprising a container vessel (2) attached to the upper end of the carrying rod (1), wherein a through hole (3) is formed in the middle of the container vessel (2), and the lower end of the through hole (3) corresponds to the biological material loading part of the carrying rod (1); the upper end of the container vessel (2) is provided with a liquid outlet groove (4), a liquid outlet cavity (5) is arranged inside the container vessel (2), and the liquid outlet groove (4) and the through hole (3) are respectively communicated with the liquid outlet cavity (5).
2. A cell freezing aid as claimed in claim 1 wherein: one end of the liquid discharge cavity (5) is communicated with the lower part of the liquid discharge groove (4), the other end of the liquid discharge cavity (5) surrounds the periphery of the through hole (3), a plurality of liquid discharge channels (6) are uniformly distributed between the through hole (3) and the liquid discharge cavity (5), and each liquid discharge channel (6) is communicated with the through hole (3) and the liquid discharge cavity (5).
3. A cell freezing aid as claimed in claim 2, wherein: each liquid drainage channel (6) is tangent to the through hole (3).
4. A cell freezing aid as claimed in claim 2 or 3 wherein: the container dish (2) comprises a transparent observation layer (201) positioned at the upper end of the liquid discharge cavity (5), and the through hole (3) and the liquid outlet groove (4) penetrate through the transparent observation layer (201).
5. A cell freezing aid according to claim 4 wherein: the upper part of the through hole (3) corresponding to the transparent observation layer (201) is of a funnel-shaped structure with the opening caliber gradually reduced from top to bottom.
6. A cell freezing aid according to claim 4 wherein: the container vessel (2) also comprises a solution treatment layer (202) positioned at the lower end of the transparent observation layer (201) and a release layer (203) positioned at the lower end of the solution treatment layer (202), and the upper end of the carrying rod (1) is attached to the lower end of the release layer (203); the liquid discharge cavity (5) and all the liquid discharge channels (6) are grooves arranged at the upper end of the solution treatment layer (202); the release layer (203) is made of an elastic material.
7. A cell freezing aid according to claim 6 wherein: the middle part of the through hole (3) corresponding to the solution processing layer (202) is of a funnel-shaped structure with the opening caliber gradually reduced from top to bottom.
8. A cell freezing aid according to claim 6 or 7 wherein: the container dish (2) is provided with a plurality of air holes (7), and the lower end of each air hole (7) is correspondingly arranged on the carrying rod (1).
9. A cell freezing aid as claimed in claim 8 wherein: the distance between each air hole (7) and the central axis of the through hole (3) is gradually reduced from top to bottom.
10. A cell freezing aid as claimed in claim 8 wherein: the lower end of the separation layer (203) is provided with an annular gas groove (8) positioned outside the through hole (3), and the lower end of each gas hole (7) is communicated with the annular gas groove (8).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021464614.2U CN212325242U (en) | 2020-07-22 | 2020-07-22 | Cell freezing auxiliary device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021464614.2U CN212325242U (en) | 2020-07-22 | 2020-07-22 | Cell freezing auxiliary device |
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| CN212325242U true CN212325242U (en) | 2021-01-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111700064A (en) * | 2020-07-22 | 2020-09-25 | 明日加加科技有限公司 | Cell freezing auxiliary device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111700064A (en) * | 2020-07-22 | 2020-09-25 | 明日加加科技有限公司 | Cell freezing auxiliary device |
| CN111700064B (en) * | 2020-07-22 | 2024-03-08 | 明日加科技(深圳)有限公司 | Cell freezing auxiliary device |
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Effective date of registration: 20230419 Address after: 905-102, Block A, Phase I, Tian'an Innovation Technology Plaza, No. 29 Tairan Fourth Road, Tian'an Community, Shatou Street, Futian District, Shenzhen City, Guangdong Province, 518000 Patentee after: Mingrijia Technology (Shenzhen) Co.,Ltd. Address before: 2c, block 16, Yan Yue, phase II, Tin Yan Bay, No. 8, Ko Road, Pak Shek Kok, Tai Po, New Territories, Hong Kong, China Patentee before: Tomorrow Jiajia Technology Co.,Ltd. |