CN220810272U - Tissue culture substance storage device - Google Patents
Tissue culture substance storage device Download PDFInfo
- Publication number
- CN220810272U CN220810272U CN202321876074.2U CN202321876074U CN220810272U CN 220810272 U CN220810272 U CN 220810272U CN 202321876074 U CN202321876074 U CN 202321876074U CN 220810272 U CN220810272 U CN 220810272U
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- China
- Prior art keywords
- tissue culture
- fixedly connected
- inner box
- storage device
- substance storage
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- 239000000126 substance Substances 0.000 title claims abstract description 26
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 46
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 23
- 230000009467 reduction Effects 0.000 claims description 9
- 210000005056 cell body Anatomy 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 3
- 230000007246 mechanism Effects 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 6
- 210000001519 tissue Anatomy 0.000 description 20
- 239000000463 material Substances 0.000 description 6
- 238000004321 preservation Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 4
- 230000007547 defect Effects 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- 206010064571 Gene mutation Diseases 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000032341 cell morphogenesis Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 239000002577 cryoprotective agent Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of tissue culture substance storage, and discloses a tissue culture substance storage device, which comprises a liquid nitrogen tank, wherein a pipeline is fixedly connected to the outside of the liquid nitrogen tank, an outer box is fixedly connected to the tail end of the pipeline, a support is arranged at the bottom of the outer box, an inner box is arranged in the outer box, a fixing frame is fixedly connected to the inside of the inner box, a clamping mechanism is arranged in the fixing frame, a culture bottle is arranged in the fixing frame, a fixing ring is fixedly connected to the outside of the culture bottle, the tissue culture substance storage device has the advantages of simple and stable structure, convenient storage and the like, the culture bottle is stored by using the structures such as the inner box and the outer box, the storage stability of the culture bottle is improved by using the clamping mechanism, shaking of the culture bottle in the storage process is avoided, and the problem of inconvenient storage and the conventional tissue culture substance storage process is solved.
Description
Technical Field
The utility model relates to the technical field of tissue culture substance storage, in particular to a tissue culture substance storage device.
Background
The ultralow temperature preservation tissue culture technology is one of effective ways for preserving germplasm, and can be used for rapidly establishing plant asexual propagation lines, and has the defects that cytologic change is required to occur in the long-term subculture process, so that chromosome variation and gene mutation are caused, and genetic instability is increased. With the development of science, scientists have combined ultra-low temperature cryopreservation technology and tissue culture technology, which have become the best way to preserve plant germplasm. Under ultra-low temperature conditions (typically liquid nitrogen low temperature, -196 ℃) almost all cellular metabolic activity and growth processes cease to proceed, but the potential for cell viability and morphogenesis is preserved, and thus the genetic stability of the cell culture is preserved. Plant materials are stored in a culture container in a tissue culture mode for transportation, and international or inter-region germplasm exchange is carried out, so that time and space can be saved, transportation cost can be reduced, and harmful organisms carried in the field seed collection or used asexual propagation materials can be reduced.
The ultralow temperature preservation is to preserve germplasm resources in an ultralow temperature environment below-80 ℃ and is a set of current biological preservation technology developed in the 70 s. Liquid nitrogen is often used as a cold source, so that ultralow temperature preservation is also called liquid nitrogen preservation. Under the condition of liquid nitrogen (-196 ℃), the addition of cryoprotectant can reduce the metabolism level of tissue culture, and is favorable for long-term preservation of cells, embryoid, test tube plantlet and callus. It has been reported that cryopreserved carrot embryoids have the ability to regenerate plants after 1 year of storage.
In actual operation, tissue culture substances are required to be placed through a containing device, then the tissue culture substances are slowly placed in a liquid nitrogen tank through a connecting rod of the containing device, and the tissue culture substances are directly refrigerated through the liquid nitrogen tank, but the process of directly storing the tissue culture substances in the liquid nitrogen tank is inconvenient, so that the tissue culture substance storage device is provided.
Disclosure of utility model
Aiming at the defect of inconvenient access in the tissue culture substance storage process in the prior art, the utility model provides a tissue culture substance storage device which has the advantages of simple and stable structure and convenient access.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a tissue culture substance storage device, includes the liquid nitrogen jar, the outside fixedly connected with pipeline of liquid nitrogen jar, the tail end fixedly connected with outer case of pipeline, the bottom of outer case is provided with the support, the inside of outer case is provided with the inner box, the inside fixedly connected with mount of inner box, the inside of mount is provided with fixture, the internally mounted of mount has the blake bottle, the outside fixedly connected with solid fixed ring of blake bottle.
Preferably, the top of the outer box is provided with a closing cap, and the distance from the bottom wall of the inner part of the outer box to the top of the box body is equal to the height of the inner box.
Preferably, the top of inner box is provided with the sign, the bottom UNICOM groove has been seted up to the bottom of inner box, the cell body has been seted up at the top of inner box, the fixed slot has been seted up at the top of inner box, the inside joint of fixed slot has the fixed stopper, can use the fixed stopper to seal the inner box simple, reaches the fixed purpose to the blake bottle simultaneously.
Preferably, the fixture includes the pivot, the pivot rotates the inside of being connected in the mount, the outside fixedly connected with gear of pivot, the outside meshing of gear is connected with the pinion rack, the back fixedly connected with movable block of pinion rack, the tail end of pivot is provided with the gear reduction box, the tail end of gear reduction box is provided with the dwang, the outside fixedly connected with connecting rod of dwang, the tail end fixedly connected with grip block of connecting rod, fixture can carry out simple centre gripping to the blake, avoids the blake to rock.
Preferably, the identification is provided with a plurality of identification, is distributed in a linear array, and can be used for indicating the species in the culture flask.
Preferably, a groove body corresponding to the culture flask is formed in the fixing frame, and the culture flask is provided with a plurality of grooves corresponding to the marks.
Preferably, the connecting rod is arranged to be of a bent rod-shaped structure, and an arc-shaped curved surface is arranged at the bottom of the clamping block.
Compared with the prior art, the utility model has the beneficial effects that:
1. This tissue culture material storage device, through the pressure valve that starts the liquid nitrogen jar, to inside the letting in liquid nitrogen of inner box, liquid nitrogen will flow in bottom UNICOM groove and cell body, and the inside material of blake bottle of placing in the mount is refrigerated, opens the closing cap when taking out, then pulls out the fixed plug, dresses protective glove or use corresponding anchor clamps take out the blake bottle can, simple structure, the access is convenient.
2. This tissue culture material storage device makes the blake bottle drive solid fixed ring extrusion movable block through the downwardly moving blake bottle, drives the pinion rack and removes to drive gear rotation through the pinion rack, the pivot rotation speed of rotation reduces through gear reduction box, the dwang rotates, the dwang drives the connecting rod and rotates, the connecting rod drives the grip block extrusion blake bottle, when the blake bottle reached interior bottom of the case, the grip block realizes the fixation to the blake bottle, when taking out, directly stimulate the blake bottle upwards move can.
Drawings
FIG. 1 is a schematic diagram of the structure of the present utility model;
FIG. 2 is a schematic view of the structure of the inner box of the utility model;
FIG. 3 is a schematic view of a retaining plug according to the present utility model;
FIG. 4 is a schematic cross-sectional view of the present utility model;
Fig. 5 is a schematic structural view of a clamping mechanism according to the present utility model.
In the figure: 1. a liquid nitrogen tank; 2. a pipe; 3. an outer case; 4. a closing cap; 5. a bracket; 6. an inner box; 7. identification; 8. a bottom communicating groove; 9. a fixing frame; 10. a tank body; 11. a fixing groove; 12. a fixed plug; 13. a culture bottle; 14. a fixing ring; 15. a clamping mechanism; 16. a rotating shaft; 17. a gear; 18. a toothed plate; 19. a moving block; 20. a gear reduction box; 21. a rotating lever; 22. a connecting rod; 23. and a clamping block.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Example 1
Referring to fig. 1-5, a tissue culture substance storage device comprises a liquid nitrogen tank 1, a pipeline 2 is fixedly connected to the outside of the liquid nitrogen tank 1, an outer box 3 is fixedly connected to the tail end of the pipeline 2, a support 5 is arranged at the bottom of the outer box 3, an inner box 6 is arranged in the outer box 3, a fixing frame 9 is fixedly connected to the inner box 6, a clamping mechanism 15 is arranged in the fixing frame 9, a culture bottle 13 is arranged in the fixing frame 9, and a fixing ring 14 is fixedly connected to the outside of the culture bottle 13.
Wherein, the top of outer tank 3 is provided with closing cap 4, and the distance of the inside diapire of outer tank 3 to box top equals the height of inner tank 6.
Wherein, the top of inner box 6 is provided with sign 7, and bottom UNICOM groove 8 has been seted up to the bottom of inner box 6, and cell body 10 has been seted up at the top of inner box 6, and fixed slot 11 has been seted up at the top of inner box 6, and the inside joint of fixed slot 11 has fixed stopper 12, can use fixed stopper 12 to seal inner box 6 simple and reach the fixed purpose to blake bottle 13 simultaneously.
Wherein, the mark 7 is provided with a plurality of, is linear array distribution, can note the inside material type of blake bottle 13 through mark 7.
Wherein, the inside of mount 9 has seted up the cell body that corresponds with blake bottle 13, and blake bottle 13 is provided with a plurality of that corresponds with sign 7.
Working principle: when the tissue culture substance storage device is used, liquid nitrogen can be introduced into the inner box 6 by starting a pressure valve of the liquid nitrogen tank 1, and the liquid nitrogen flows in the bottom communicating groove 8 and the groove body 10 to refrigerate substances in the culture bottle 13 placed in the fixing frame 9; when the culture flask is required to be taken out, the closing cap 4 is opened, then the fixing plug 12 is pulled out, protective gloves are worn, or the culture flask 13 is taken out by using a corresponding clamp, so that the structure is simple, and the storage and the taking are convenient.
Example two
Referring to fig. 1-5, further on the basis of the first embodiment, the clamping mechanism 15 includes a rotating shaft 16, the rotating shaft 16 is rotatably connected to the inside of the fixing frame 9, a gear 17 is fixedly connected to the outside of the rotating shaft 16, a toothed plate 18 is connected to the outside of the gear 17 in a meshed manner, a moving block 19 is fixedly connected to the back of the toothed plate 18, a gear reduction box 20 is arranged at the tail end of the rotating shaft 16, a rotating rod 21 is arranged at the tail end of the gear reduction box 20, a connecting rod 22 is fixedly connected to the outside of the rotating rod 21, a clamping block 23 is fixedly connected to the tail end of the connecting rod 22, the connecting rod 22 is in a curved rod structure, an arc-shaped curved surface is arranged at the bottom of the clamping block 23, and the clamping mechanism 15 can simply clamp the culture bottle 13 to avoid shaking of the culture bottle 13.
Working principle: when the tissue culture substance storage device is used, the culture bottle 13 can be enabled to drive the fixed ring 14 to extrude the moving block 19 through downwards moving the culture bottle 13, the toothed plate 18 is driven to move, the toothed plate 18 is used for driving the gear 17 to rotate, the gear 17 is used for driving the rotating shaft 16 to rotate, the rotating speed of the rotating shaft 16 is reduced through the gear reduction box 20, the rotating rod 21 is rotated, the rotating rod 21 is used for driving the connecting rod 22 to rotate, the connecting rod 22 is used for driving the clamping block 23 to extrude the culture bottle 13, when the culture bottle 13 reaches the bottom of the inner box 6, the clamping block 23 is used for fixing the culture bottle 13, and when the culture bottle 13 is taken out, the culture bottle 13 is directly pulled to upwards move, so that the simple fixing of the culture bottle 13 is completed, and shaking is avoided.
Claims (6)
1. A tissue culture substance storage device, comprising a liquid nitrogen tank (1), characterized in that: the utility model provides a liquid nitrogen container, outside fixedly connected with pipeline (2) of liquid nitrogen container (1), the tail end fixedly connected with outer case (3) of pipeline (2), the bottom of outer case (3) is provided with support (5), the inside of outer case (3) is provided with inner box (6), the inside fixedly connected with mount (9) of inner box (6), the inside of mount (9) is provided with fixture (15), the internally mounted of mount (9) has blake bottle (13), the outside fixedly connected with solid fixed ring (14) of blake bottle (13), fixture (15) include pivot (16), pivot (16) swivelling joint is in the inside of mount (9), the outside fixedly connected with gear (17) of pivot (16), the outside meshing of gear (17) is connected with pinion rack (18), the back fixedly connected with movable block (19) of pinion rack (18), the tail end of pivot (16) is provided with gear reduction box (20), the tail end of gear reduction box (20) is provided with dead lever (21), the outside of connecting rod (22) is connected with connecting rod (22).
2. A tissue culture substance storage device according to claim 1, wherein: the top of outer case (3) is provided with closing cap (4), the inside diapire of outer case (3) is equal to the height of inner box (6) to the distance at box top.
3. A tissue culture substance storage device according to claim 1, wherein: the top of inner box (6) is provided with sign (7), bottom UNICOM groove (8) have been seted up to the bottom of inner box (6), cell body (10) have been seted up at the top of inner box (6), fixed slot (11) have been seted up at the top of inner box (6), the inside joint of fixed slot (11) has fixed stopper (12).
4. A tissue culture substance storage device according to claim 3, wherein: the marks (7) are distributed in a linear array.
5. A tissue culture substance storage device according to claim 1, wherein: the inside of mount (9) is seted up the cell body that corresponds with blake bottle (13), blake bottle (13) are provided with a plurality of that corresponds with sign (7).
6. A tissue culture substance storage device according to claim 1, wherein: the connecting rod (22) is of a bent rod-shaped structure, and an arc-shaped curved surface is arranged at the bottom of the clamping block (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321876074.2U CN220810272U (en) | 2023-07-17 | 2023-07-17 | Tissue culture substance storage device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321876074.2U CN220810272U (en) | 2023-07-17 | 2023-07-17 | Tissue culture substance storage device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220810272U true CN220810272U (en) | 2024-04-19 |
Family
ID=90699164
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321876074.2U Active CN220810272U (en) | 2023-07-17 | 2023-07-17 | Tissue culture substance storage device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220810272U (en) |
-
2023
- 2023-07-17 CN CN202321876074.2U patent/CN220810272U/en active Active
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