CN208791609U - Deep-Sea Microorganisms water body in-situ culture apparatus - Google Patents

Abstract

The utility model relates to marine microorganism cultivation technology field more particularly to a kind of Deep-Sea Microorganisms water body in-situ culture apparatus, including upper top cover, shell, fixed part, bracket component and multiple culture units；Shell has an inner cavity, and the pick-and-place mouth being connected to inner cavity, upper top cover and cage connection are formed on shell and picks and places mouth for being closed；Several recirculation holes being connected to inner cavity are offered on shell；Fixed part setting is on the shell and for being hung on abysmal area for shell is attached；Multiple culture units are respectively used to contain microbiological culture media and can make microbiological culture media and extraneous progress mass exchange；Bracket component is used to multiple culture units being fixed on inner cavity；Rigid protective structure is formed by setting fixed part, shell and upper top cover, the water flow of underwater complex or big biology is avoided to damage the culture apparatus inside and outside；By the way that bracket component, effective protection culture unit, so that the culture apparatus can carry out the long-term cultivation of microorganism in deep-marine-environment is arranged.

Description

Deep-Sea Microorganisms water body in-situ culture apparatus

Technical field

The utility model relates to marine microorganism cultivation technology fields, specifically, being to be related to one kind attached for a long time to hang over Microorganism water body in-situ culture apparatus in the water body environment of deep-sea.

Background technique

Abysmal area refers to that the depth of water is low temperature (0~4 DEG C), high pressure (more than 100 atmosphere in the ocean of 1000m or more Pressure) and low dissolved oxygen extreme environment, carry out Deep-Sea Microorganisms resource research be develop ocean, utilize ocean genetic resources Important component.In recent years, due to the development of deep-sea detecting technology, so that its Mysterious Veil has gradually been opened in the deep-sea world. Deep-Sea Microorganisms are formed in the evolutionary process for adapting to complex environment (generally high pressure, low temperature, dark, with high salt, oligotrophic etc.) Unique hereditary metabolic mechanism and chemical defense mechanisms；Currently, only extremely least a portion of deep-sea microorganisms in water is in laboratory It is separated or enriched out in culture, the main reason is that laboratory environment can not simulate home state completely, lacks certain slightly The necessary factor of biological growth, and most microorganism growths are extremely slow, and the fast fast-growing in part is less competitive than in laboratory environment Long bacterium, some microorganisms then need to coexist with other microorganisms and could grow.In soil (and deposit) research, largely Culture in situ technology is applied very well, but due to the mobility of water body, has more changing factors compared with soil, Therefore water body in-situ culture technique faces bigger challenge, so correlative study development is less, especially for deep layer original position seawater Long-term cultivation experiment is almost without correlative study also can be only applied to the experiment of sea surface Short-term Culture.

It follows that existing culture technique is mostly that laboratory is separately cultured, but its limitation is very big, exist very big random Property, and most objective microbes are difficult to cultivate；In recent years, Culture in situ technology obtains large development, though it can be to a certain extent Solve the problems, such as unknown microculture limitation, but the device of attachable in-situ enrichment long-term into marine environment lacks it at present Again few, also only limitation is applied to that the long-term cultivation of deep-marine-environment can not be adapted to compared with shallow water area mostly.

In addition, in the correlative study of edaphon enrichment culturing in situ, because of Bio-(one kind is for original by beads The porous beads of position culture Atrazine bacterium colony, please refer to " In-situ enrichment and analysis of atrazine-degrading microbial communities using atrazine-containing porous Beads " etc. academic articles and opinions) it is internal have relatively to be independent microenvironment and be the several months stablize release nutriment and wide General application.But in water body in-situ culture, using porous beads (abbreviation beads) carry out correlative study almost without.

In short, developing a kind of anti-interference corrosion resistant water body in-situ enrichment dress when carrying out Culture in situ experiment in water body It is particularly necessary for setting.

Utility model content

The purpose of this utility model is to provide a kind of Deep-Sea Microorganisms water body in-situ culture apparatus, it is intended to solve existing skill The technical issues of water body in-situ culture apparatus in art can not carry out microorganism long-term cultivation in deep-marine-environment.

For this purpose, the technical solution adopted in the utility model is: providing a kind of Deep-Sea Microorganisms water body in-situ culture Device, including upper top cover；Shell has an inner cavity, is formed with pick-and-place mouth in communication with the inner cavity on the shell, described Upper top cover and the cage connection simultaneously are used to be closed the pick-and-place mouth；It is offered on the shell in communication with the inner cavity several Recirculation hole；Fixed part, setting is on the housing and for being hung on abysmal area for the shell is attached；Multiple culture units, point Microbiological culture media Yong Yu not contained and the microbiological culture media and extraneous progress mass exchange can be made；And bracket group Part, for multiple culture units to be fixed on the inner cavity.

Further, the bracket component includes the culture bracket being arranged in the inner cavity and is embedded in culture branch Multiple containing tubes on frame, each containing tube all have an inner space, multiple culture units and multiple receipts Pipe of receiving corresponds respectively, and multiple culture units are separately positioned in the inner space of the corresponding containing tube；It is described The outer wall of containing tube is formed with several mesh.

Further, the culture bracket includes loading plate, isolation board and is connected to the loading plate and is isolated with described Supporting element between plate, the loading plate and the isolation board interval are arranged；The carrying is formed through on the loading plate Multiple fixing grooves of plate, multiple containing tubes correspond respectively with multiple fixing grooves, the week of multiple containing tubes It is abutted against respectively with the cell wall of the corresponding fixing groove to outer wall.

Further, the loading plate forms the central channel through the loading plate at the center of the loading plate, Central channel periphery equidistant arranged for interval of the multiple fixing grooves of the loading plate respectively at the loading plate；The center The notch shape of the notch shape of slot and multiple fixing grooves is circle, and the containing tube is cylindrical.

Further, the loading plate and the isolation board are stainless steel plate, and the supporting element is stainless steel cylinder branch Support member.

Further, the shell is cylindrical, and one end of the shell is arranged in the upper top cover；The loading plate and The isolation board is in the form of annular discs, and the cross sectional radius size of the loading plate and the cross sectional radius size of the isolation board are equal Less than the cross sectional radius size of the shell.

Further, the cross sectional radius size of the isolation board is not less than the cross sectional radius size of the loading plate.

Further, the culture bracket is multiple, and multiple culture brackets are via the pick-and-place mouth in the inner cavity In successively arrange；It is each it is described culture bracket supporting element be it is multiple, it is each it is described culture bracket multiple supporting elements pass through Wear the loading plate of the culture bracket and the equidistant arranged for interval in central channel periphery respectively at the culture rack bearing plate.

Further, the shell is stainless steel casing.

Further, the opening being connected with the inner space is formed on the containing tube, the containing tube has The end cap of the opening is connect and is used to be closed with the containing tube；The containing tube is stainless steel mesh pipe.

The beneficial effect of Deep-Sea Microorganisms water body in-situ culture apparatus provided by the utility model is: with the prior art pair Than, the utility model proposes a kind of Deep-Sea Microorganisms water body in-situ culture apparatus, including upper top cover, shell, fixed part, bracket Component and multiple culture units, wherein multiple culture units are multiple beads cultivating systems mentioned above, pass through setting Fixed part, shell and upper top cover make whole device have rigid protective structure, avoid the water flow or big raw of underwater complex Object damages the culture apparatus inside and outside；By the way that bracket component is arranged, it is effectively protected beads cultivating system, in turn So that water body in-situ culture apparatus can carry out the long-term cultivation of microorganism in deep-marine-environment.

Detailed description of the invention

It, below will be to embodiment or the prior art in order to illustrate more clearly of the technical scheme in the embodiment of the utility model Attached drawing needed in description is briefly described, it should be apparent that, the accompanying drawings in the following description is only that this is practical new Some embodiments of type for those of ordinary skill in the art without any creative labor, can be with It obtains other drawings based on these drawings.

Fig. 1 is the stereoscopic schematic diagram of shell provided by the embodiment of the utility model, upper top cover and fixed part；

Fig. 2 is the stereoscopic schematic diagram of culture bracket provided by the embodiment of the utility model；

Fig. 3 is the schematic top plan view of culture bracket provided by the embodiment of the utility model；

Fig. 4 is the stereoscopic schematic diagram of containing tube provided by the embodiment of the utility model.

Wherein, each appended drawing reference in figure:

1: upper top cover

2: shell 210: inner cavity

220: picking and placing mouth 230: recirculation hole

3: fixed part 31: upper fixed part

32: lower fixed part

41: culture bracket 42: containing tube

421: inner space 422: mesh

411: loading plate 412: isolation board

413: supporting element 414: fixing groove

410: central channel 420: opening

423: end cap

Specific embodiment

The embodiments of the present invention are described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning Same or similar element or element with the same or similar functions are indicated to same or similar label eventually.Below by ginseng The embodiment for examining attached drawing description is exemplary, it is intended to for explaining the utility model, and should not be understood as to the utility model Limitation.

In addition, term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or Implicitly include one or more of the features.

It is to be appreciated that term " length ", " width ", "upper", "lower", "front", "rear", "left", "right", "vertical", The orientation or positional relationship of the instructions such as "horizontal", "top", "bottom", "inner", "outside" is that orientation based on the figure or position are closed System, is merely for convenience of describing the present invention and simplifying the description, rather than the device or element of indication or suggestion meaning are necessary It with specific orientation, is constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.

In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " Gu It is fixed " etc. terms shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integral；It can be Mechanical connection, is also possible to be electrically connected；It can be directly connected, two can also be can be indirectly connected through an intermediary The interaction relationship of connection or two elements inside element.It for the ordinary skill in the art, can basis Concrete condition understands the concrete meaning of above-mentioned term in the present invention.

It as shown in Figures 1 to 4, is a preferred embodiment provided by the utility model.

A kind of Deep-Sea Microorganisms water body in-situ culture apparatus (abbreviation water body in-situ culture apparatus) provided in this embodiment, packet Upper top cover 1, shell 2, fixed part 3, bracket component and multiple culture units are included, specifically, shell 2 has an inner cavity 210, outside The pick-and-place mouth 220 being connected to inner cavity 210 is formed on shell 2, upper top cover 1 connect with shell 2 and picks and places mouth 220 for being closed, with Realize that the water body in-situ culture apparatus has a more closed protection structure, specifically, referring to Fig. 1, in the present embodiment, Shell 2 and upper top cover 1 are one end that shell 2 is arranged in stainless steel material and upper top cover 1, more specifically, the two can be by 316 Anticorrosion stainless steel is cast, and 2 shape of shell is cylindric in one, and thickness is about 2~3mm, upper and lower basal diameter (i.e. D1 in Fig. 1) About 110mm, 2 height (i.e. H1 in Fig. 1) of shell may be configured as 250~270mm, preferably 260mm；Upper top cover 1 and shell 2 Be fixedly connected and can be realized by anti-skidding bolt, can also be realized certainly by modes such as engaging, buckle, threaded connections, herein not Make unique limit；In addition, several recirculation holes being connected to inner cavity 210 can be offered on shell 2, to guarantee that seawater in situ can It circulates inside and outside the water body in-situ culture apparatus, recirculation hole may extend over entire shell 2 and be arranged, or even extend to upper top cover 1 Place, in the present embodiment, circulation bore dia be but be not limited to 5mm, and the quantity and arrangement mode of specific recirculation hole are herein not Make unique limit；Fixation portions 3 is arranged on shell 2 and for being hung on abysmal area for shell 2 is attached, in the present embodiment, Gu Fixed part 31 and once fixed part 32, upper fixed part 31 can be divided on one be arranged on the same bus of cylinder-like shell 2 by determining portion 3 Be arranged close to upper top cover 1, lower fixed part 32 is arranged close to bottom, both for but the polyethylene material that is not limited to after polishing Cable holding part, non-skid property is good and can still make it when water body in-situ culture apparatus is hit by water impact or big biology Stablize and attached is hung on abysmal area；Multiple culture units are respectively used to contain microbiological culture media and can make microbiological culture media and outer Boundary carries out mass exchange, and in the present embodiment, culture unit is porous beads formula cultivating system (i.e. beads cultivating system), should Culture unit can provide stable Culture in situ environment for deep-sea quasi-microorganism, so that microorganism keeps relatively in environment in situ The accuracy of independent growth, Culture in situ experiment is higher；Certainly, culture unit is also chosen as mini dialysis pocket type according to demand Or the small device of other types Culture in situ, it does not limit uniquely herein；In addition, bracket component can be used for consolidating multiple culture units Due to inner cavity 210；Make whole device that there is rigid protective structure by setting fixed part 3, shell 2 and upper top cover 1, keeps away The water flow or big biology for having exempted from underwater complex damage the culture apparatus inside and outside；By the way that bracket component is arranged, effectively Beads cultivating system and the integrally-built stability of above-mentioned water body in-situ culture apparatus, anti-interference and corrosion resistance are protected, So that water body in-situ culture apparatus can carry out the long-term cultivation of microorganism in deep-marine-environment, on the one hand deepen for deep The long-term cultivation of layer seawater in situ tests project, has on the other hand then filled up and has carried out water body in-situ training using beads cultivating system Support the blank of research field.

Further, referring to Figure 2 together to Fig. 4, bracket component include the culture bracket 41 being arranged in inner cavity 210 and The multiple containing tubes 42 being embedded on culture bracket 41, each containing tube 42 all have an inner space 421, multiple culture units It is corresponded respectively with multiple containing tubes 42, multiple culture units are separately positioned in the inner space 421 of corresponding containing tube 42； The outer wall of containing tube 42 is formed with several mesh 422；Further, referring to Fig. 4, being formed on containing tube 42 and inner space 421 openings 420 being connected, containing tube 42 have the end cap 423 connecting with containing tube 42 and for being closed opening 420；Storage Pipe 42 can be enclosed by stainless (steel) wires more than 200 mesh and form cylinder-like structure, and the connection type of end cap 423 and containing tube 42 is herein It does not limit uniquely；Specifically, the basal diameter (i.e. D2 in Fig. 4) of containing tube 42 can be 25mm, and 42 height of containing tube (is schemed H4 in 4) it can be 59mm or so, which is not only effectively protected the beads in pipe, also it is effectively prevent therefrom to fall Out, meanwhile, which interact the beads cultivating system in containing tube 42 sufficiently with surrounding seawater, ensure that experimental data Accuracy.

Further, please refer to figs. 2 and 3, and culture bracket 41 includes that loading plate 411, isolation board 412 and being connected to is held Supporting element 413 between support plate 411 and isolation board 412, loading plate 411 and the interval of isolation board 412 are arranged, specifically, in this reality It applies in example, the two is parallel and arranged for interval；Multiple fixing grooves 414 of loading plate 411 are formed through on loading plate 411, it is multiple Containing tube 42 corresponds respectively with multiple fixing grooves 414, the peripheral outer wall of multiple containing tubes 42 respectively with corresponding fixing groove 414 Cell wall abut against；In the present embodiment, loading plate 411, isolation board 412 are 316 stainless steel disk formula plates, the two uniform thickness Degree and thickness are about 2~3mm, and supporting element 413 is 316 stainless steel cylindrical supports, cross sectional radius (i.e. Fig. 3 of supporting element 413 In R2) be about 5mm, height (i.e. H2 in Fig. 2 and H3 and summation) is about 60mm；Further, please continue to refer to Fig. 2 and figure 3, loading plate 411 forms the central channel 410 through loading plate 411, specifically, central channel 410 at the center of loading plate 411 Notch radius (i.e. R1 in Fig. 3) be about 19mm, multiple fixing grooves 414 of loading plate 411 are respectively at the center of loading plate 411 The equidistant arranged for interval in 410 periphery of slot；The notch shape of the notch shape of central channel 410 and multiple fixing grooves 414 is circle, Each 414 notch radius of fixing groove (i.e. R3 in Fig. 3) is 12.5~13.5mm, is chosen as 13mm, and preferably, a culture There are six identical fixing grooves 414 for the tool of bracket 41；It, can by the way that the size and matching relationship of fixing groove 414 and containing tube 42 is arranged Guarantee being bonded for culture bracket 41 and containing tube 42, avoid containing tube 42 when crust of the device 2 is by external interference because Culture bracket 41 fixing groove 414 in up and down play and be damaged, i.e., the structure effectively increase culture bracket 41 and storage The structural stability and service life of pipe 42；By be arranged central channel 410 so that containing tube 42 preferably with the sea of lateral flow Water carries out mass exchange, to preferably carry out enrichment culturing in situ experimental study to microorganism.

It should be noted that the cross sectional radius size of loading plate 411 and the cross sectional radius size of isolation board 412 are small In the cross sectional radius size of shell 2, consequently facilitating loading plate 411, isolation board 412 are mounted in the inner cavity 210 of shell 2； And preferably, the cross sectional radius size of isolation board 412 is not less than the cross sectional radius size of loading plate 411, in this way, isolation board 412 can bear most impacts perpendicular to longitudinal water flow of loading plate 411 to loading plate 411 and containing tube 42, Jin Ergeng The containing tube 42 on loading plate 411 is protected well, in the present embodiment, for the ease of loading plate 411, the batch of isolation board 412 Processing and manufacturing, loading plate 411, the cross sectional radius size (i.e. R4 in Fig. 3) of isolation board 412 are identical and be 50mm or so.

Further, culture bracket 41 is multiple, and multiple culture brackets 41 are layer-by-layer in inner cavity 210 via mouth 220 is picked and placed Arrangement, it is preferable that multiple culture brackets 41 are specially four；The supporting element 413 of each culture bracket 41 is multiple, Mei Yipei The multiple supporting elements 413 for supporting bracket 41 are carried through the loading plate 411 of the culture bracket 41 and respectively at the culture bracket 41 The equidistant arranged for interval in 410 periphery of central channel of plate 411, it is preferable that there are six the branch of identical size for each culture bracket 41 tool Support member 413.In specific arrangement, each loading plate 411 for cultivating bracket 41 and isolation board 412 can be handed over four culture brackets 41 by sequence The arranged direction of mistake arrangement, i.e., each culture bracket 41 need to be consistent；For example, if the four cultures branch successively arranged from top to bottom Frame 41 is respectively labeled as the first culture bracket (not shown), the second culture bracket (not shown), third culture bracket (not shown) Bracket (not shown) is cultivated with the 4th, at this point, the support plate of four culture brackets 41 can be located on itself isolation board 412 (can also be respectively positioned under itself isolation board 412 for the support plate of four culture brackets 41 certainly), therefore at this point, the second culture bracket One end of each supporting element 413 support in the second culture bracket isolation board 412 towards the second culture rack bearing plate 411 On surface, the other end supports the isolation board 412 in the first culture bracket towards on the surface of second support loading plate 411, the One culture bracket is located at the second culture rack bearing plate 411 away from the side of the second culture bracket isolation board 412；Similarly, It supports in third culture bracket isolation board 412 towards third culture bracket one end of each supporting element 413 of three culture brackets On the surface of loading plate 411, the other end supports the isolation board 412 in the second culture bracket towards third rack bearing plate 411 Surface on, second culture bracket is located at third culture rack bearing plate 411 away from third culture bracket isolation board 412 one Side；Similarly, one end of each supporting element 413 of the 4th culture bracket, which is supported, cultivates 412 direction of bracket isolation board the 4th On the surface of 4th culture rack bearing plate 411, the other end supports the isolation board 412 in third culture bracket towards the 4th On the surface of frame loading plate 411, third culture bracket is located at the 4th culture rack bearing plate 411 away from the 4th culture bracket isolation The side of plate 412；It should be noted that the distance between loading plate 411 and isolation board 412 of each culture bracket 41 (i.e. Fig. 2 In H3) may be configured as 35mm, two neighboring culture bracket 41, such as isolation board 412 and the second culture of the first culture bracket The distance between loading plate 411 of bracket (i.e. H2 in Fig. 2) is about 25mm；By successively arranging multiple trainings in inner cavity 210 Support bracket 41, on the one hand, multiple culture brackets 41, which mutually support support, ensure that the stability of each culture 41 structure of bracket, another Aspect increases cultivating system quantity in the water body in-situ culture apparatus, is conducive to screening experiment result not only with guarantee test knot Fruit accuracy can also meet the diversity requirement of microorganism water body in-situ culture；Meanwhile the structure may make each culture bracket Each containing tube 42 on 41 can come into full contact with the seawater of lateral flow, and isolation board 412 has then preferably completely cut off different interlayers The cross-infection of cultivating system ensure that the accuracy of experimental result.

Utility model aims to solve the enrichment culturing in situ problems of microorganism in the water body environment of deep-sea；It is combining On the basis of beads culture technique, this water body in-situ culture apparatus not only can guarantee stable Culture in situ environment, but also can effectively protect Destruction of the culture medium from big biology for protecting beads and its inside makes microorganism keep relatively independent growth in environment in situ； Water body in-situ culture apparatus can beads cultivating system inside effective protection, and package unit is rigid protective structure, is avoided The water flow of underwater complex and big biology and the destruction to culture apparatus, device have small in volume, can be flexible according to depth Carry out the culture studies of different water levels, and tests its development and recycling using the Culture in situ that the water body in-situ culture apparatus carries out Process is relatively simple and convenient, and package unit can also carry out high-temperature sterilization and then realize to repeatedly use, utilization ratio of device Height, experimental cost are low.It should be noted that this water body in-situ culture apparatus is attempted to cultivate in the mouth of the Zhujiang River island Gui Shan waters, and Its visible 2 portion body device of shell and 2 interior brace assemblies part of shell are intact after device recycling after 1 year, furtherly The structural stability and exploitativeness of this water body in-situ culture apparatus is illustrated.

Obviously, the above embodiments of the present invention is used for the purpose of clearly illustrating examples for clearly illustrating the present invention, and It is not limitations of the embodiments of the present invention.For those of ordinary skill in the art, in above description On the basis of can also make other variations or changes in different ways.There is no need and unable to give all embodiments Exhaustion.Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention etc., should be included in Within the protection scope of the utility model claims.

Claims (10)

1. a kind of Deep-Sea Microorganisms water body in-situ culture apparatus characterized by comprising

Upper top cover；

Shell, has an inner cavity, is formed with pick-and-place mouth in communication with the inner cavity on the shell, the upper top cover with it is described Cage connection simultaneously is used to be closed the pick-and-place mouth；Several recirculation holes in communication with the inner cavity are offered on the shell；

Fixed part, setting is on the housing and for being hung on abysmal area for the shell is attached；

Multiple culture units are respectively used to contain microbiological culture media and can make the microbiological culture media and extraneous progress object Mass transter；And

Bracket component, for multiple culture units to be fixed on the inner cavity.

2. Deep-Sea Microorganisms water body in-situ culture apparatus according to claim 1, which is characterized in that the bracket component packet The multiple containing tubes for including the culture bracket being arranged in the inner cavity and being embedded on the culture bracket, each containing tube An inner space is all had, multiple culture units correspond respectively with multiple containing tubes, and multiple cultures are single Member is separately positioned in the inner space of the corresponding containing tube；The outer wall of the containing tube is formed with several mesh.

3. Deep-Sea Microorganisms water body in-situ culture apparatus according to claim 2, which is characterized in that the culture bracket packet Include loading plate, isolation board and the supporting element being connected between the loading plate and the isolation board, the loading plate with it is described The setting of isolation board interval；Be formed through multiple fixing grooves of the loading plate on the loading plate, multiple containing tubes with Multiple fixing grooves correspond respectively, the peripheral outer wall of multiple containing tubes respectively with the slot of the corresponding fixing groove Wall abuts against.

4. Deep-Sea Microorganisms water body in-situ culture apparatus according to claim 3, which is characterized in that the loading plate in Form the central channel for running through the loading plate at the center of the loading plate, multiple fixing grooves of the loading plate respectively at The equidistant arranged for interval in central channel periphery of the loading plate；The slot of the notch shape of the central channel and multiple fixing grooves Mouth-shaped is circle, and the containing tube is cylindrical.

5. Deep-Sea Microorganisms water body in-situ culture apparatus according to claim 3, which is characterized in that the loading plate and institute Stating isolation board is stainless steel plate, and the supporting element is stainless steel cylindrical support.

6. Deep-Sea Microorganisms water body in-situ culture apparatus according to claim 3, which is characterized in that the shell is in cylinder One end of the shell is arranged in shape, the upper top cover；The loading plate and the isolation board are in the form of annular discs, the loading plate Cross sectional radius size and the cross sectional radius size of the isolation board be respectively less than the cross sectional radius size of the shell.

7. Deep-Sea Microorganisms water body in-situ culture apparatus according to claim 6, which is characterized in that the cross of the isolation board Section radius size is not less than the cross sectional radius size of the loading plate.

8. according to the described in any item Deep-Sea Microorganisms water body in-situ culture apparatus of claim 3~7, which is characterized in that described Cultivate bracket be it is multiple, multiple culture brackets are successively arranged in the inner cavity via the pick-and-place mouth；Each training Support bracket supporting element be it is multiple, it is each it is described culture bracket multiple supporting elements run through the culture bracket loading plate and Respectively at the equidistant arranged for interval in central channel periphery of the culture rack bearing plate.

9. Deep-Sea Microorganisms water body in-situ culture apparatus according to claim 8, which is characterized in that the shell is stainless Outer steel shell.

10. Deep-Sea Microorganisms water body in-situ culture apparatus according to claim 8, which is characterized in that on the containing tube It is formed with the opening being connected with the inner space, the containing tube is described with connecting with the containing tube and being used to be closed The end cap of opening；The containing tube is stainless steel mesh pipe.