CN101928059A - Device for rapidly culturing biological films in natural water body - Google Patents
Device for rapidly culturing biological films in natural water body Download PDFInfo
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- CN101928059A CN101928059A CN2010100011826A CN201010001182A CN101928059A CN 101928059 A CN101928059 A CN 101928059A CN 2010100011826 A CN2010100011826 A CN 2010100011826A CN 201010001182 A CN201010001182 A CN 201010001182A CN 101928059 A CN101928059 A CN 101928059A
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- carbon fiber
- activated carbon
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- water body
- microbial film
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Abstract
The invention relates to a device for rapidly culturing biological films in natural water body, which consists of a disc carrier and an active carbon fiber fixing disc, wherein the disc carrier comprises discs (1), a fixed column (2), a rope penetrating hole (3) and a connecting hole (4); an active carbon fiber fixing device comprises a ring-shaped gasket (5), a fixed hole (6) and active carbon fiber (7); the active carbon fiber fixing disc is fixed on the disc carrier by a fixing rope (8) through the rope penetrating hole (3); and the discs are connected with each other by the fixing rope (8) through a connecting hole (4). The device is mainly used for rapidly culturing the biological films in natural water body in batches. The device can be used for culturing the biological films which are even and close to the natural condition, have high quality, and are used for ecological monitoring, can shortens the cultivation time to be more than 20 days, and can culture the biological films at the different depths in the water body at the same time.
Description
Technical field
The present invention relates to environmental pollution monitoring and evaluation and ecological recovery field, be used for ecological monitoring, can realize fast biomembranous cultivation and collection in the natural water body.
Background technology
Microbial film can be grown on any interface in the water, as stone, settling, various waterplant etc., mainly is made up of algae, bacterium, fungi and miniature organism and mucopolysaccharide matrix.Microbial film is owing to distribute extensively, and volume is little, and life cycle is short, to various artificial interference sensitivities, becomes the important means of water quality biological monitoring and evaluation in recent years gradually.Though and the microbial film that is grown on the natural substrates is easy to obtain, but because the difference that the difference of matrix often causes biofilm structure to form, error in causing water quality monitoring (WQM) easily and estimating, expose cultivation so be applied to ecological monitoring research at present based on artificial substratum, the matrix that is most widely used is simple glass plate, synthetic glass or nylon wire, rubble etc.But adopt the foster microbial film required time of these substrate cultures just can collect enough biomasss and can be used to test definite water quality and ecosystem health state than length.Findlay and Sinsabaugh (2006) adopt rubble to expose in the river as matrix and cultivate the variation that 5-6 week collection of biological film is estimated biological metabolism; Duong etc. (2006) adopt sheet glass to expose 4 all collection of biological films in urban river and are used for estimating the influence of municipal pollution to diatom group; Fellows etc. (2006) adopt nylon wire to expose the end that 4 all collection of biological films are used for determining the river metabolic evaluation ecosystem health of dwelling.People's publication numbers such as Dong Deming are the microbial film culture apparatus in batches that discloses a kind of glass carrier in the patent of CN2461930.But with glass, nylon or rubble is that matrix cultivate to be collected and to be had following two aspect inferior positions when microbial film in the natural water body is used for water quality assessment: it is longer that (1) needs expose incubation time, is unfavorable for reflecting fast the water environment pollution variation; (2) because conditions such as biofilm development and illumination, temperature are closely related, therefore, different depths biofilm structure, changes of function are significant in the research water body, and the prior biological film is cultivated the microbial film that gathering device can not be gathered different depths in the water body simultaneously in batches.
Purpose of the present invention is exactly in order to overcome above-mentioned deficiency, makes up one and overlaps fast the natural water body microbial film and cultivate gathering device in batches.
Summary of the invention
The purpose of this invention is to provide a kind of can the fast culture natural water body in biomembranous device.This device can solve in the natural water body microbial film and cultivate length consuming time, and same device can not be gathered the biomembranous problem of different depths water body.
For achieving the above object, the present invention has adopted following technical scheme:
Biomembranous culture apparatus is made of disc carrier and gac stationary installation two portions in the natural water body fast.Disc carrier is made by polymethylmethacrylate, comprise disk (1) and fixed leg (2) composition, a plurality of fixed legs (2) stick in disk (1) periphery uniformly, on fixed leg (2), get out lacing hole (3), the height of lacing hole (3) omits height a bit than annular gasket (5) thickness, so that fixing activated carbon fiber shaft collar, four connecting holes of sidetracking (4) outside disk (1), a plurality of culture apparatuses are used for connecting.The activated carbon fiber shaft collar is made up of annular gasket (5) and activated carbon fiber (7), and annular gasket (5) is made by polymethylmethacrylate, evenly makes a call to 4 fixed orificess (6) on ring segment, in order to activated carbon fiber to be installed.Activated carbon fiber (7) is filled in the fixed orifices (6), the annular gasket (5) that will fix activated carbon fiber (7) then is enclosed within on the fixed leg (2), static line (8) is fixed on the gac shaft collar on the disc carrier by lacing hole (3) successively, with cotton rope a plurality of disks are together in series by connecting hole again, can be used for cultivating the microbial film of different depths in the natural water body.
Effect of the present invention and benefit are that because activated carbon fiber (7) has porousness, the characteristics that specific surface area is big can be adsorbed the settled organism in the natural water body fast, have solved the long difficult problem of microbial film incubation time in the natural water body.Owing to the transparent material that is that adopts, light can freely see through, and more near state of nature, is beneficial to biofilm development.A plurality of disks can be together in series among the present invention, and can regulate the needed degree of depth as required and carry out the microbial film cultivation.
The present invention is simple in structure, practical, compares with other functionally similar devices to have characteristics such as with low cost, convenient and swift.The microbial film that can be widely used in various natural water bodies is cultivated, and is applicable to environmental science and ecological monitoring field.
Description of drawings
Provided setting drawing of the present invention among Fig. 1, Fig. 2 is the vertical view of disc carrier, and Fig. 3 is the annular gasket vertical view.
Wherein, 1 is that disk 2 is connecting hole for fixed leg 3 for lacing hole 4
5 is that annular gasket 6 is static line for fixed orifices 7 for activated carbon fiber 8
Embodiment
Be described in detail principle of the present invention and embodiment based on accompanying drawing below.
The present invention is made up of disk (1), fixed leg (2), lacing hole (3), connecting hole (4), annular gasket (5), fixed orifices (6), activated carbon fiber (7) and static line (8).Fig. 1 is the two groups of culture apparatuses of microbial film fast that can adorn 20 2cm * 10cm activated carbon fiber.Disk among Fig. 2 (1) is made by polymethylmethacrylate, diameter 30cm, the fixed leg (2) that 5 synthetic glass of adhesion are made on it, and at position brill 4 connecting holes (4) near disk border 1cm, can be used for disk being joined to one another or coupling together with kickboard etc., fixed leg (2) bottom surface diameter 1cm, high 2cm highly locates to bore lacing hole (3) at fixed leg (2) apart from disk 0.6cm, and annular gasket among Fig. 3 (5) is made by synthetic glass, external diameter 6cm, the about 1.2cm of internal diameter, thickness 0.5cm can be enclosed within on the fixed leg (2), go up apart from even 4 fixed orificess (6) that bore in the position of outer rim 0.5cm at annular gasket (5), bore dia is 0.5cm.Activated carbon fiber (7) specific surface area is 1500m
2/ g is cut into little of the rectangle of the wide 2cm of long 10cm, the one end is tucked in 4 fixed orificess (6) on the annular gasket (5), again it is enclosed within on the fixed leg (2), also tie a knot by lacing hole (3) successively with static line (8), be fixed on the disc carrier, allow activated carbon fiber dangle naturally, a plurality of disks are coupled together by connecting hole (4), and can the present invention be fixed on buoy or other fixtures by static line (8), this device is suspended in the natural water body.
Embodiment is in bar river, Beijing, utilize the present invention as the microbial film culture apparatus, compare with prior biological film artificial substratum cultural method, monitoring time is that August is to September, test period temperature is 26-34 ℃, because the river is more shallow, only hangs one deck disk, and each microbial film culture apparatus is hung on 20cm place, underwater.Its microbial film culture effect is as shown in Table 1 and Table 2:
Table 1 different substrates attached biological film biomass (ash dry weight) is (g/m relatively
2)
The average growth velocity of microbial film is 1.04mg (AFDW)/m on 30 days activated carbon fiber
2D
-1, be 3.5 times of biofilm development speed on glass of smearing glass cement, be 1.7 times of synthetic glass matrix.The ash dry weight of the biomass after activated carbon fiber was cultivated in 7 days has exceeded the microbial film on glass of smearing glass cement, incubation time can be shortened greatly.
Relatively (ten thousand/cm of the algae density of table 2 different substrates attached biological film
2)
Activated carbon fiber enrichment algae ability will be higher than glass far away, even the quantity of enrichment algae is exactly more than 2 times of 30 days enrichment numbers of gluing sheet glass in the time of first day, adopt the activated carbon fiber algae in the enrichment natural water body rapidly, and be used for water quality monitoring (WQM).
The algae structure distribution of table 3 different substrates attached biological film is (%) relatively
| ? | Green alga | Diatom | Blue-green algae and other | The advantage algae |
| Glass+glass cement was cultivated 30 days | 74.1? | 9.0? | 16.9? | The water silk floss |
| Synthetic glass was cultivated 30 days | 75.2? | 14.1? | 10.7? | The water silk floss |
| Activated carbon fiber was cultivated 7 days | 68.8? | 11.7? | 19.5? | The water silk floss |
By algae structure distribution in the different substrates attached biological film in the table 3 as can be seen, though it is rapid that activated carbon fiber is used for the ecological monitoring enrichment as the foster microbial film of substrate culture, incubation time is short, but does not change the structure of periphtic algae, and the advantage algae is still based on the water silk floss.Use SPSS13.0 that algae structure distribution on activated carbon fiber and gluing glass and the synthetic glass is carried out the significant difference analysis, obtain p=0.135 and p=0.223, p＞0.1 illustrates that activated carbon fiber and gluing glass, synthetic glass algae structure distribution do not have significant difference.
Claims (6)
1. microbial film culture apparatus in the natural water body fast, it is characterized in that in various natural water bodies, make up the biomembranous fast culture device of a kind of natural water body, the activated carbon fiber shaft collar is set on disc carrier, activated carbon fiber is fixed on the disc carrier by annular gasket, utilize the activated carbon fiber specific surface area big, the characteristics of high adsorption capacity make algae in the natural water body, bacterium, protobiont fast attached on the activated carbon fiber.
2. the microbial film culture apparatus is by disk (1) in the quick natural water body according to claim 1, fixed leg (2), and lacing hole (3), connecting hole (4), annular gasket (5), fixed orifices (6), activated carbon fiber (7) and static line (8) constitute.The disk that described disc carrier is made by polymethylmethacrylate (1) and evenly stick in 5 fixed legs (2) on the disk (1) and be evenly distributed on 4 connecting holes (4) in the disk outside and the lacing hole (3) on the fixed leg (2) and static line (8) are formed; The gac shaft collar is made up of annular gasket (5) and activated carbon fiber (7), comprises 4 carbon fiber fixed orificess (6) on the annular gasket (5).Activated carbon fiber (7) is suspended on the annular gasket by fixed orifices (6), the annular gasket (6) of having fixed activated carbon fiber is placed on the fixed leg (2), with static line (8) successively by the lacing hole (3) on the fixed leg (2), the annular gasket that has hung activated carbon fiber is fixed on the disk, again a plurality of disks (1) are joined to one another by connecting hole (4) with static line (8), then can be used for cultivating simultaneously the microbial film in the different depths water body.
3. according to claim 1 and 2, natural water body microbial film fast culture process is characterized in that adopting activated carbon fiber to support microbial film as substrate culture.
4. according to claim 1 and 2, activated carbon fiber is fixed on the disc carrier by annular gasket, can cultivate many group microbial films in batches, and maintains a certain distance (about 4 to 4.5 centimetres) between activated carbon fiber each other, help biomembranous growth, make microbial film look more even.
5. according to claim 1 and 2, owing to adopt transparent material to make, light can freely see through, and more near state of nature, is beneficial to biofilm development.
6. according to claim 1 and 2, disk can be together in series by connecting hole, cultivates the microbial film of the different depth of waters, and it is long to regulate rope as required, so that obtain the microbial film of the required degree of depth.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539635A (en) * | 2012-01-05 | 2012-07-04 | 中国农业大学 | Simulating and monitoring device for health status of wetland water ecological system |
| CN102849845A (en) * | 2012-08-27 | 2013-01-02 | 北京化工大学 | Preparation method and application of biological carbon fiber water treatment filler |
| CN103071399A (en) * | 2013-01-09 | 2013-05-01 | 河海大学 | Composite flat membrane with regeneration capacity and preparation method thereof |
| CN103304034A (en) * | 2013-06-04 | 2013-09-18 | 浙江大学 | Method and device for purifying water body with heavy-metal pollution |
| CN104229981A (en) * | 2014-09-03 | 2014-12-24 | 嘉兴安立得建设工程有限公司 | Carbon fiber water repair ecological system |
| CN108102880A (en) * | 2018-02-02 | 2018-06-01 | 云南大学 | A kind of biological film culture device and its application method |
| CN108165457A (en) * | 2017-12-29 | 2018-06-15 | 北京师范大学 | Biological habitat insitu analog device in a kind of water body |
| CN108751388A (en) * | 2018-06-20 | 2018-11-06 | 东北师范大学 | It is a kind of to be used in environment water using micro- plastics as the biological film culture device of matrix |
| CN111548920A (en) * | 2020-04-03 | 2020-08-18 | 河海大学 | Biological membrane culture experimental device capable of automatically controlling working conditions and culture method thereof |
| CN112062281A (en) * | 2020-07-30 | 2020-12-11 | 浙江工业大学 | Method for repairing arsenic pollution of saline-alkali water body by using blue algae-biomembrane complex |
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2010
- 2010-01-15 CN CN2010100011826A patent/CN101928059A/en active Pending
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539635B (en) * | 2012-01-05 | 2014-09-17 | 中国农业大学 | Simulating and monitoring device for health status of wetland water ecological system |
| CN102539635A (en) * | 2012-01-05 | 2012-07-04 | 中国农业大学 | Simulating and monitoring device for health status of wetland water ecological system |
| CN102849845A (en) * | 2012-08-27 | 2013-01-02 | 北京化工大学 | Preparation method and application of biological carbon fiber water treatment filler |
| CN103071399A (en) * | 2013-01-09 | 2013-05-01 | 河海大学 | Composite flat membrane with regeneration capacity and preparation method thereof |
| CN103071399B (en) * | 2013-01-09 | 2015-01-14 | 河海大学 | Composite flat membrane with regeneration capacity and preparation method thereof |
| CN103304034A (en) * | 2013-06-04 | 2013-09-18 | 浙江大学 | Method and device for purifying water body with heavy-metal pollution |
| CN104229981A (en) * | 2014-09-03 | 2014-12-24 | 嘉兴安立得建设工程有限公司 | Carbon fiber water repair ecological system |
| CN104229981B (en) * | 2014-09-03 | 2015-11-25 | 嘉兴安立得建设工程有限公司 | Carbon fiber water repairs the ecosystem |
| CN108165457B (en) * | 2017-12-29 | 2021-03-26 | 北京师范大学 | In-situ simulation device for biological habitat in water body |
| CN108165457A (en) * | 2017-12-29 | 2018-06-15 | 北京师范大学 | Biological habitat insitu analog device in a kind of water body |
| CN108102880A (en) * | 2018-02-02 | 2018-06-01 | 云南大学 | A kind of biological film culture device and its application method |
| CN108102880B (en) * | 2018-02-02 | 2023-11-24 | 云南大学 | Biological film culture device and application method thereof |
| CN108751388A (en) * | 2018-06-20 | 2018-11-06 | 东北师范大学 | It is a kind of to be used in environment water using micro- plastics as the biological film culture device of matrix |
| CN111548920A (en) * | 2020-04-03 | 2020-08-18 | 河海大学 | Biological membrane culture experimental device capable of automatically controlling working conditions and culture method thereof |
| CN111548920B (en) * | 2020-04-03 | 2022-11-08 | 河海大学 | Biological membrane culture experimental device capable of automatically controlling working conditions and culture method thereof |
| CN112062281A (en) * | 2020-07-30 | 2020-12-11 | 浙江工业大学 | Method for repairing arsenic pollution of saline-alkali water body by using blue algae-biomembrane complex |
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Application publication date: 20101229 |