CN108102880B - Biological film culture device and application method thereof - Google Patents
Biological film culture device and application method thereof Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
The application discloses a biological film culture device and a use method thereof, wherein the biological film culture device comprises two hanging rods which are oppositely arranged, and a closed control switch, a closing device and a biological film culture device which are sequentially arranged between the two hanging rods from top to bottom along the hanging rods, the closing device comprises a first closing device and a second closing device, the first closing device and the second closing device are hollow cavity structures with first openings, one ends of the first closing device and the second closing device with the first openings are sleeved on a rotating shaft and are connected in a rotating mode, and when the first closing device and the second closing device are in a closing state, the biological film culture device is sealed in the first closing device and the second closing device. The biological film culture device has the technical effects that when the biological film culture device is taken out of water, the biological film can be prevented from being damaged by water flow when the biological film culture device moves upwards, and the accuracy of data is prevented from being influenced.
Description
Technical Field
The application belongs to the technical field of environmental science, and particularly relates to a biological film culture device and a using method thereof.
Background
In recent years, with the continuous and deep research on microorganisms in different fields, the survival period, community structure and corresponding succession rules of the microorganisms have become the most effective and direct means for detecting and evaluating the health condition of the aquatic ecosystem. Compared with detection methods such as physical chemistry, the biological detection is more sensitive and accurate in evaluating the influence of different environmental factors and different pollutants on the aquatic ecosystem.
More than 90% of microorganisms in the water body are attached to the surface of the living or inanimate solid matrix in the form of biological films. Biofilms are composed of microbial populations (including bacteria, protozoa, fungi, etc.), inorganic minerals, and organic polymer matrices (extracellular polymers and humus). The biological film has higher surface reactivity and larger pollutant adsorption area. The agglomeration, adsorption and degradation processes of contaminants in biological membranes reflect a combination of physical, chemical and biological purification, which plays an important role in the self-cleaning process of water. The structure and composition of the biofilm reflects the long-term evolution of the community, structure and function of aquatic microorganisms. It may be the most effective strategy for monitoring and assessing the health of aquatic ecosystems. Thus, biofilms adhering to the surfaces of various substrates in rivers and lakes are one of the indicators describing the health of aquatic ecosystems. However, researches on biological membranes are focused on sewage treatment, water supply and drainage and other engineering fields, and researches on natural water biological membranes such as lakes, rivers and the like are less. In addition, due to the limitations of experimental equipment and methods, the research on the biological film of the natural water body is mostly limited to the surfaces of natural matrixes such as lakes, rivers, wetlands and the like with shallow water depths, which are easy for the biological film to adhere to, grow and collect, and the research on the biological film of the synchronous microorganism in the deep water depth area and underwater multiple depths is freshly reported.
At present, bacterial biofilms in natural water bodies are mainly collected destructively in shallow water areas of the natural water bodies by means of cell scrapers, knives or brushes and the like, and original morphological structure characteristics and functions of the biofilms are difficult to maintain. On the other hand, considering that the depth of a part of deep water lakes and reservoirs can reach 200-300 meters, how to culture and collect biological membranes with different water depths and complete morphological structures is a difficult point which is mainly needed to be overcome when the biological membranes are used for researching the morphological structures and functions of the biological membranes.
Disclosure of Invention
It is an object of the present application to provide a biofilm culturing device.
According to one aspect of the application, a biological membrane culturing device is provided, which comprises two hanging rods oppositely arranged, and a sealing control switch, a sealing device and a biological membrane culturing device which are sequentially arranged along the hanging rods from top to bottom between the two hanging rods, wherein the sealing device comprises a first sealing device and a second sealing device, the first sealing device and the second sealing device are hollow cavity structures with first openings, one ends of the first sealing device and the second sealing device with the first openings are sleeved on a rotating shaft to be rotationally connected, the biological membrane culturing device is arranged on the rotating shaft, two ends of the rotating shaft are respectively fixed on the two hanging rods, an opening state and a closing state are arranged between the first sealing device and the second sealing device, when the biological membrane culturing device is in the opening state, the first sealing device and the second sealing device are in sealing communication through the two first openings, the biological membrane culturing device is sealed in the first sealing device and the second sealing device, the control switch is configured to enable the first sealing device and the second sealing device to be in the opening state, and the second sealing device is released from the opening state and the second sealing device when the first sealing device is in the opening state, and the second sealing device is in the opening state, and the opening state is released from the opening state.
Optionally, a collection box is located below the biofilm incubator and has an upper opening larger than the biofilm incubator, the collection box being connected to the first or/and the second closure, the collection box being configured to move upwardly to cover the biofilm incubator when rotated from an open state to a closed state.
Optionally, the collection box further has a lower opening provided with a cross support structure.
Optionally, the biological membrane culturing device further comprises a lower baffle plate, wherein the lower baffle plate is fixed at the lower ends of the two hanging rods, and the cross section of the lower baffle plate in the horizontal direction is larger than that of the biological membrane culturing device.
Optionally, a hanging ring is arranged below the biofilm culturing device, and the hanging ring is configured for hanging a balancing weight.
Optionally, the first sealer or/and the second sealer has a quarter-circle sector structure in a cross section perpendicular to a rotational axis, the rotational axis passing through a circle of the sector.
Optionally, a cushion layer is disposed at the first opening.
Optionally, the closed control switch includes the connecting rod, the both ends of connecting rod are fixed respectively on two peg, be provided with lifting hammer, lift spring, prop claw and two dead levers on the connecting rod, the lifting hammer cover is established on the connecting rod and can reciprocate for the connecting rod, be provided with lift spring drive lifting hammer between lifting hammer and the connecting rod and upwards keep away from the connecting rod, the one end of two dead levers respectively with first closure ware and second closure ware fixed connection, when first closure ware and second closure ware are fixed to be in the open state, the other end of two dead levers is blocked respectively in propping claw locking, the lifting hammer is configured to be in for when the connecting rod moves down, can release the propping claw to the locking of dead lever.
Optionally, the biofilm culturing device is provided with an upper plate and a lower plate which are opposite, and a frame structure with transparent periphery, wherein a plurality of pairs of opposite parallel grooves are arranged on the upper plate and the lower plate, and two glass slides for biofilm growth are clamped in each pair of grooves.
According to another aspect of the present application, there is also provided a method for using the above-mentioned biofilm culturing device, comprising the steps of:
when the biomembrane culture device is applied to water, the first sealer and the second sealer are opened;
after the biomembrane is cultured, the locking of the first sealer and the second sealer is released by controlling the sealing control switch;
the first sealer and the second sealer reach a closing state, and the biological film incubator is sealed;
the biofilm culturing device is taken out of the water upwards.
The biological film culture device has the technical effects that when the biological film culture device is taken out of water, the biological film can be prevented from being damaged by water flow when the biological film culture device moves upwards, and the accuracy of data is prevented from being influenced.
Drawings
The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application and do not constitute a limitation on the application. In the drawings:
FIG. 1 is a schematic elevational view of one embodiment of the present application;
FIG. 2 is a schematic side view of one embodiment of the present application;
FIG. 3 is a schematic side view of a closed control switch according to one embodiment of the present application;
FIG. 4 is a schematic elevational view of a closed control switch according to one embodiment of the present application;
FIG. 5 is a schematic elevational view of a closed control switch according to another embodiment of the present application;
FIG. 6 is a schematic side view of a biofilm incubator in accordance with an embodiment of the present application;
FIG. 7 is a schematic diagram showing the front view of a biofilm incubator in accordance with an embodiment of the present application.
FIG. 8 is a schematic perspective view of a biofilm incubator in accordance with an embodiment of the present application.
In the figure: the biological membrane culturing device comprises a lifting hammer 1, a fixed rod 2, a sealing device 3, a sealing device 31, a sealing device 32, a biological membrane culturing device 4, a collecting box 5, a hanging rod 6, a supporting claw 7, a fixed pulley 8, a lifting spring 9, a hook 10, a lifting ring 11, a lower baffle 12, a connecting wire 13, a glass slide 14, a screw 15, a groove 16, a rotating shaft 17, a connecting rod 18, a steel rope 19, a torsion spring 20 and a fixed iron block 21.
Detailed Description
The following will describe embodiments of the present application in detail by referring to examples, so that the implementation process of how to apply the technical means to solve the technical problems and achieve the technical effects of the present application can be fully understood and implemented.
The biological film culture device provided by the application can prevent the biological film from being damaged by water flow when the biological film culture device is taken out from water and the accuracy of data is influenced.
The application provides a biological film culture device, which is shown in figures 1-2, and comprises two hanging rods 6 which are oppositely arranged, and a closed control switch, a closed device and a biological film culture device 4 which are sequentially arranged between the two hanging rods 6 from top to bottom along the hanging rods 6.
The closure device comprises a closure 3. The closure 3 comprises a first closure 31 and a second closure 32. The first and second sealers 31, 32 are hollow cavity structures each having a first opening. One end of the first sealer 31 and one end of the second sealer 32, which are provided with the first openings, are sleeved on the rotating shaft 17 to be connected in a rotating way, so that the first sealer 31 and the second sealer 32 can rotate relatively, namely, the two first openings can be close to or far away from each other, and when the two first openings are close to each other, if the two first openings correspond to each other, the two first openings can rotate to be completely opposite to each other, and the first sealer 31 and the second sealer 32 are sealed at the same time, so that a sealed accommodating cavity is formed together. The two ends of the rotating shaft 17 can be respectively fixed on the two hanging rods 6, so that the whole structure of the device is more stable, and the load is born by the hanging rods 6.
The biofilm incubator 4 is disposed on the rotating shaft 17 for culturing biofilm at a proper depth. The rotation shaft 17 may be perpendicular to the hanging rod 6, and the biofilm culturing device 4 may be hung on the rotation shaft 17 through a steel rope 19 arranged at the center line position of the biofilm culturing device 4, and the biofilm culturing device 4 may be horizontally fixed on the rotation shaft 17 by means of self-gravity, or the application is not limited thereto.
The first and second sealers 31 and 32 have an opened state and a closed state therebetween, and as shown in fig. 1-2, the first and second sealers 31 and 32 are in the opened state, and the biofilm culturing device 4 is exposed to the outside. When the first and second sealers 31 and 32 are in the closed state, that is, as the first and second sealers 31 and 32 are rotated relative to each other, the two first openings are moved closer to each other until they overlap, and the first and second sealers 31 and 32 are in sealed communication with each other through the two first openings, so that the biofilm culturing device 4 is sealed in the first and second sealers 31 and 32. When the device is used, the first sealer 31 and the second sealer 32 are firstly arranged in an open state, the biofilm culturing device is placed into water through the rope and located at a preset depth, for example, the length of the rope is preset, after the biofilm culturing is completed, the first sealer 31 and the second sealer 32 are in a closed state, the sealing protection of the biofilm culturing device 4 is completed, and when the biofilm culturing device is taken out from the water slowly and upwards, the biofilm is prevented from being damaged by water flow when the biofilm culturing device moves upwards, and the accuracy of data is affected. The integrity of the biological film can be ensured, the sampling accuracy is facilitated, and the morphological structure of the collected biological film is kept complete.
The closing control switch is used for locking the first closing device 31 and the second closing device 32 to be in an open state, and simultaneously, when the first closing device 31 and the second closing device 32 are subjected to proper and enough external force, the locking can be released, so that the first closing device 31 and the second closing device 32 can rotate from the open state to the closed state, and the biological film culture device can move upwards and be taken out of water without affecting the integrity of the morphological structure of the biological film.
Optionally, as shown in fig. 1-2, a collection box 5 is also included. The collecting box 5 is located below the biofilm culturing device 4 and has an upper opening larger than the biofilm culturing device 4, and can be used for accommodating the biofilm culturing device 4 when the biofilm culturing device 4 is matched, and protecting the periphery of the biofilm culturing device 4, so as to prevent water flow impact on the biofilm in the biofilm culturing device 4 when the first sealing device 31 and the second sealing device 32 are closed. The collection cassette 5 is connected to the first sealer 31 and/or the second sealer 32, and the collection cassette 5 moves upward to cover the biofilm incubator 4 when rotated from an open state to a closed state. For example, as shown in fig. 1-2, the hook 10 is provided on the first closure 31 or/and the second closure 32, and the fixed pulley 8 fixed between the two hanging rods 6 is connected with the bottom end of the collecting box 5 through the connecting wire 13 (which may be various traction wires such as steel wires, ropes, etc.), so that the collecting box 5 can be driven to move upwards when the first closure 31 and the second closure 32 are rotated from the opened state to the closed state. The fixed pulley 8 is fixed above the rotating shaft 17. The hanger 10 may be fixed to the first sealer 31 or/and the end of the second sealer 32 having the first opening. The collecting box 5 is not connected to the hanger bar 6.
Optionally, as shown in fig. 5, the collecting box 5 also has a lower opening provided with a cross support structure, i.e. a hollow structure in the lower part thereof, for reducing the resistance to and the disturbance to the water flow that is experienced when moving upwards.
Optionally, as shown in fig. 1-2, a lower baffle 12 is also included. The lower baffle 12 is fixed at the lower ends of the two hanging rods 6, and the cross section of the lower baffle in the horizontal direction is larger than that of the biological film incubator 4, so as to prevent the impact on the biological film incubator 4 and the floating of the collection box 5 caused by the upward buoyancy of the water body from influencing the culture effect of the biological film incubator 4 in the placing process of the biological film collection device and the culture process in water. The collecting box 5 may be pre-glued to the lower baffle 12, but with a smaller adhesion force than the pulling force of the first closure 31 and/or the second closure 32 on the collecting box 5.
Optionally, as shown in fig. 1-2, a lifting ring 11 is provided below the biofilm culturing device, the lifting ring 11 being used to suspend a counterweight, in cooperation with a rope, so as to ensure that the whole biofilm culturing device is at a desired water depth during biofilm growth. The balancing weight can be a lead fish, etc.
Alternatively, the biofilm culturing device is completely exposed to the outside when the first and second sealers 31 and 32 are in an opened state.
Optionally, when the first and second sealers 31, 32 are in the opened state, the first openings are both directed downward.
Alternatively, as shown in fig. 1-2, the first closure 31 and/or the second closure 32 have a quarter-circle sector structure in a cross section perpendicular to the rotation axis 17, the rotation axis 17 passing through the sector's circle.
Optionally, a cushion layer is arranged at the first opening, so that the impact force of the sealer when the sealer is closed can be reduced, and the sealer can be tightly closed and sealed. .
Alternatively, as shown in fig. 1-5, the closed control switch includes a connecting rod 18, two ends of the connecting rod 18 are respectively fixed on the two hanging rods 6, a lifting hammer 1, a lifting spring 9, a supporting claw 7 and two fixing rods 2 are arranged on the connecting rod 18, the lifting hammer 1 is sleeved on the connecting rod 18 and can move up and down relative to the connecting rod 18, the lifting hammer 1 is driven by the lifting spring to move up and down relative to the connecting rod 18, one ends of the two fixing rods 2 are respectively fixedly connected with a first sealer 31 and a second sealer 32, when the first sealer 31 and the second sealer 32 are fixed in an open state, the other ends of the two fixing rods 2 are respectively clamped in the supporting claw 7 to be locked, and when the lifting hammer 1 is subjected to an external force and moves down relative to the connecting rod 18, the locking of the supporting claw 7 to the fixing rods 2 can be released. The connecting rod 18 is parallel to the rotation shaft 17. The connecting rod 18 is provided with a rope which passes through the lifting hammer 1 and extends to be fixed on the water surface. The biofilm culturing device is fixedly connected with the upper part of the water surface through a rope on the connecting rod 18, so that the biofilm culturing device is convenient to find and control. The closed control switch may be a locking hook switch as shown in fig. 4-5, the two supporting claws 7 are in a splayed structure, the torsion springs 20 respectively arranged at opposite ends of the two supporting claws 7 drive the two supporting claws 7 to rotate in a direction of making an angle between the two supporting claws larger, the lifting hammer 1 is provided with fixed iron blocks 21 at positions corresponding to the two supporting claws 7, a clamping groove is formed between the fixed iron blocks 21, the width of the clamping groove is determined, when the lifting hammer 1 moves downwards relative to the connecting rod 18, the clamping groove drives the two supporting claws 7 to rotate in a direction of making an angle between the two supporting claws smaller, so that the fixed rod 2 is separated from the supporting claws 7, and the rotation mode is just like the aforementioned sealer, and is not repeated herein. It is also possible to provide a locking hook switch as shown in fig. 5, and the supporting claw 7 moves downward along with the lifting hammer 1, so that the supporting claw 7 can not catch the fixing rod 2 any more, and further, one skilled in the art can design other locking switch mechanisms, which is not limited in this respect.
Alternatively, as shown in FIGS. 6 to 8, the biofilm culturing vessel 4 is a frame structure having opposite upper and lower plates and having a periphery transparent so that water can flow in the biofilm culturing vessel 4 to culture to obtain a desired biofilm structure. And the upper plate and the lower plate are provided with a plurality of pairs of parallel grooves 16 which are opposite, and two glass slides 14 for biofilm growth or other materials and structures for biofilm growth are clamped in each pair of grooves 16.
Alternatively, as shown in fig. 6 to 8, a metal mesh is provided around the biofilm culturing member 4, and a metal mesh opposite to the extending end of the groove 16 is detachably provided on the biofilm culturing member 4. For example, the biological film incubator 4 has a cuboid structure, the front and back metal meshes of the biological film incubator 4 are respectively fixed on the biological film incubator 4 through four detachable screws 15 so as to take and place the glass slide, and the two side metal meshes are directly fixed on the biological film incubator 4. For preventing damage to the slide 14 in the biofilm incubator 4 by large impurities or fish or the like.
The method of use of one embodiment shown in fig. 1-8, comprises the steps of:
when the biomembrane culture device is applied to water, the sealer 3 needs to be in an open state, namely the fixing rod 2 is arranged in the supporting claw 7 and is locked. The collection box 5 is now under the biofilm grower 4, which is fully exposed to the body of water.
The balancing weight at the bottom of the biological film culture device can ensure that the whole device is kept vertical to water flow in the water body and is submerged to a target position. The whole biofilm culturing device can be hung on a buoy or placed in a region with larger depth in the center of a water body preferably through a steel cable fixed between supports on two sides.
After a period of incubation (10-15 days), the plumb is put down along a rope (which is pre-fixed on the water surface or can be fixed on the rope in a plurality of blocks in a combined way) penetrating through a small hole at the top of the lifting hammer 1 and connected with the whole sampling device, the rope needs to be smooth and knotless so as to ensure that the plumb can strike the lifting hammer 1 smoothly, and the lifting hammer 1 is driven to move downwards relative to the connecting rod 18.
The lock of the closure 3 is released, and the closure 3 is rotated from the open state to the closed state.
After the closure 3 is completely closed, the biofilm incubator 4 is enclosed within the sealed cavity formed by the closure 3.
Slowly lifting the biofilm culturing device out of the water surface, removing the glass slide 14 in the biofilm culturing device 4, and completing the collection or observation of the biofilm.
While the foregoing description illustrates and describes several preferred embodiments of the application, it is to be understood that the application is not limited to the forms disclosed herein, but is not to be construed as limited to other embodiments, and is capable of use in various other combinations, modifications and environments and is capable of changes or modifications within the spirit of the application described herein, either as a result of the foregoing teachings or as a result of the knowledge or skill of the relevant art. And that modifications and variations which do not depart from the spirit and scope of the application are intended to be within the scope of the appended claims.
Claims (1)
1. A method of using a biofilm culturing device, comprising the steps of:
when the biomembrane culture device is applied to water, the first sealer and the second sealer are opened;
after the biomembrane is cultured, the locking of the first sealer and the second sealer is released by controlling the sealing control switch;
the first sealer and the second sealer reach a closing state, and the biological film incubator is sealed;
taking out the biological film culture device from the water upwards;
the biological film culture device comprises two hanging rods which are oppositely arranged, and a closed control switch, a closing device and a biological film culture device which are sequentially arranged between the two hanging rods from top to bottom along the hanging rods, wherein the closed device comprises a first closed device and a second closed device, the first closed device and the second closed device are hollow cavity structures with first openings, one ends of the first closed device and the second closed device with the first openings are sleeved on a rotating shaft to be rotationally connected, the biological film culture device is arranged on the rotating shaft, two ends of the rotating shaft are respectively fixed on the two hanging rods, an opening state and a closing state are arranged between the first closed device and the second closed device, when the biological film culture device is in the opening state, the biological film culture device is exposed to the outside, when the biological film culture device is in the closing state, the first closed device and the second closed device are sealed in the first closed device through the two first openings, the closed control switch is configured to lock the first closed device and the second closed device, and when the first closed device is locked, the first closed device is unlocked, and when the second closed device is unlocked, and the first closed device is opened and closed, and the second closed state is opened and closed, and the open state is released;
the closed control switch comprises a connecting rod, two ends of the connecting rod are respectively fixed on the two hanging rods, a lifting hammer, a lifting spring, a supporting claw and two fixing rods are arranged on the connecting rod, the lifting hammer is sleeved on the connecting rod and can move up and down relative to the connecting rod, the lifting spring is arranged between the lifting hammer and the connecting rod to drive the lifting hammer to move upwards and away from the connecting rod, one ends of the two fixing rods are respectively fixedly connected with a first sealer and a second sealer, when the first sealer and the second sealer are fixed in an open state, the other ends of the two fixing rods are respectively clamped in the supporting claw and are locked by the supporting claw, and when the lifting hammer moves downwards relative to the connecting rod, the locking of the supporting claw on the fixing rods can be released;
further comprising a collection box located below the biofilm incubator and having an upper opening larger than the biofilm incubator, the collection box being connected to the first or/and the second closure, the collection box being configured to move upwardly to cover the biofilm incubator when rotated from an open state to a closed state;
the collection box also has a lower opening provided with a cross support structure;
the biological membrane culturing device also comprises a lower baffle plate, wherein the lower baffle plate is fixed at the lower ends of the two hanging rods, and the cross section of the lower baffle plate in the horizontal direction is larger than that of the biological membrane culturing device;
a hanging ring is arranged below the biological film culture device and is configured to suspend a balancing weight;
the first sealer or/and the second sealer are in a sector structure of a quarter circle in a cross section perpendicular to a rotating shaft, and the rotating shaft passes through the circle of the sector;
a buffer cushion layer is arranged at the first opening;
the biological film incubator is provided with an upper plate and a lower plate which are opposite, and a frame structure with four transparent sides, wherein a plurality of pairs of opposite parallel grooves are arranged on the upper plate and the lower plate, and two glass slides for biological film growth are clamped in each pair of grooves.
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| CN111548920B (en) * | 2020-04-03 | 2022-11-08 | 河海大学 | Biological membrane culture experimental device capable of automatically controlling working conditions and culture method thereof |
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