CN218298173U - Biofouling dynamic simulation experiment device - Google Patents
Biofouling dynamic simulation experiment device Download PDFInfo
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- CN218298173U CN218298173U CN202221658934.0U CN202221658934U CN218298173U CN 218298173 U CN218298173 U CN 218298173U CN 202221658934 U CN202221658934 U CN 202221658934U CN 218298173 U CN218298173 U CN 218298173U
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Abstract
The utility model belongs to the technical field of the biological stained research, concretely relates to biological stained dynamic simulation experimental apparatus, experimental apparatus specifically include circulating filtration system, lighting system, biological culture system, adhere to test evaluation system. The utility model provides a can accomplish the cultivation of common aquatic organism in ocean and the fresh water and to the device that different materials's developments anti biological stained adhesion ability simulation and survey research under the different water environment carries out, the preparation method is simple, low price, and convenient operation, need not professional power equipment, be applicable to indoor simulation experiment, and can cultivate multiple different common aquatic organism simultaneously, it is difficult to the biological stained situation that reflects natural environment and the defect that indoor set is difficult to carry out dynamic simulation to have compensatied current indoor experiment, the efficiency of indoor simulation experiment has been improved.
Description
Technical Field
The utility model belongs to the technical field of the biological stained research, concretely relates to biological stained dynamic simulation experiment device.
Background
Biofouling refers to the accumulation of living organisms on submerged surfaces, is most common in seawater environments, such as large-area attachment of marine organisms such as seaweed and mussels to the bottoms of ships, and also relates to attachment pollution of equipment probes such as water quality monitoring (such as dissolved oxygen monitoring, pH value monitoring, temperature monitoring) by fresh water algae such as short-staple algae and gossypium hirsutum. Biofouling has great harm to marine and fresh water equipment, can directly reduce the ship speed of marine ships and accelerate fuel consumption, and attached biosecretion substances can also directly accelerate corrosion of ship bottom metal, so that the service safety of the ships is threatened, and the biofouling of measuring equipment in fresh water culture can directly cause the distortion of measured data, thereby influencing the decision of culture personnel and the delivery of nutrients, and needing to consume a large amount of manpower and financial resources every year to remove dirt on a sensor.
With the continuous development of water quality detection and water traffic fields, more and more attention is paid to water area state monitoring (such as dissolved oxygen monitoring, pH monitoring, temperature monitoring, etc.). Meanwhile, equipment such as a water quality automatic on-line analyzer and a water quality detection probe are widely applied, water quality detection is known to be an important basis for guaranteeing water quality health, and thousands of sets of water quality detection equipment are sold every year, but the water quality detection probe is often attached to various microorganisms (including brevetia algae, gossypium algae and the like) when being soaked in ocean and fresh water for a long time, so that data measured by a sensor is distorted, and a large amount of manpower and financial resources are consumed every year to remove dirt on the sensor.
The actual field test can truly reflect the anti-biofouling effect of the developed material, but the test cost is high, the risk is high, the period is long, and more importantly, the effect on certain fouling organisms cannot be accurately determined, so that the development of the biofouling evaluation of the simulated environment in the laboratory is very important. In a traditional laboratory biofouling simulation, indexes such as a hydrophobic angle and the like are adopted to indirectly reflect the antifouling capacity of a material, or a culture solution containing certain specific organisms (such as algae, bacteria and the like) is adopted to research the antifouling capacity of the material, so that a real biofouling environment is difficult to simulate, the antifouling capacity of the prepared material cannot be evaluated in real time, more importantly, the material with biofouling is usually applied to dynamic environments, such as sailing ships, flowing ocean currents and the like, and an indoor device is difficult to simulate the biofouling condition of the material under the actual dynamic application environments.
SUMMERY OF THE UTILITY MODEL
To the problem that exists among the prior art, the utility model provides a simple structure, convenient operation, running cost are low, can reflect the experimental apparatus of the stained condition of living beings under the true environment, and this experimental apparatus can place multiple material and can accomplish the cultivation of multiple aquatic organism simultaneously and the test of stained living beings in the surperficial dynamic adhesion condition of different materials.
Specifically, the utility model discloses a biofouling evaluation experimental apparatus includes circulating filtration system, illumination system, biological culture system, adheres to test evaluation system.
The biological culture system comprises a cylindrical container, an oxygen supply air pump, a temperature controller and a thermometer, wherein the cylindrical container can be made of polytetrafluoroethylene and other materials. Oxygen suppliment air pump, thermostat, thermometer set up inside the cylinder container, through the temperature of the water temperature in the thermostat control system to the temperature that is most suitable aquatic organism to grow, through thermometer monitoring water temperature, provide oxygen for the biology in the biological culture system through the oxygen suppliment air pump.
The cylindrical container is internally provided with a water sample of a water body to be researched, which can be a fresh water sample or a seawater sample, and the water tank also comprises an introduction substrate (attached with a substrate of aquatic organisms capable of causing biological fouling, such as algae, stones, stock solution and the like) and common aquatic organisms (referring to organisms which can survive in the water body environment to be researched and provide nutrient substances for an ecosystem), so that a simple ecosystem is formed, and long-time attachment test and observation can be carried out.
Preferably, the heating device of the temperature controller is a heating rod made of explosion-proof quartz glass, so that the safety of the heating rod can be improved.
The circulating filtration system comprises a water inlet pipe, a filter vat, a water pump and a water outlet pipe, wherein filter cotton or/and biochemical filter materials are arranged in the filter vat, physical or/and biochemical filtration can be performed on water entering the filter vat, the water inlet pipe and the water outlet pipe are communicated with the interior of the filter vat and a cylindrical container, and the water pump is arranged on the water inlet pipe. When the circulating filtration system operates, the water pump pumps out water in the cylindrical container, and the water is filtered physically or/and biochemically in the filter vat and then returns to the inside of the cylindrical container through the water outlet pipe.
One construction of filter vat that may be employed is as follows: including pipe, biochemical filter media baffle, a plurality of check, bung, discharge valve of intaking, biochemical filter media baffle sets up the lower extreme in the filter vat, a plurality of check that intake set up biochemical filter media baffle upper portion in the filter vat, the check of intaking are separated with the grid each other, and the check of intaking sets up the filter pulp inside.
Set up discharge valve on the filter vat bung, discharge valve air inlet and the inside intercommunication of filter vat, the gas outlet intercommunication filter vat is outside.
One end of the water inlet guide pipe is communicated with the water inlet pipe, the other end of the water inlet guide pipe is communicated with the bottom of the biochemical filter material partition plate, the water entering the filter barrel is introduced into the bottom of the biochemical partition plate through the water inlet guide pipe, and the water entering the filter barrel is filtered by the biochemical filter material partition plate and the filter cotton on the plurality of water inlet grids from bottom to top and returns to the cylindrical container through the water outlet pipe communicated with the top.
When the filter vat is used, the water inlet pipe is firstly inserted into water, the exhaust valve on the vat cover is opened, the filter vat is vacuumized through the exhaust valve by means of air pumps and the like, water enters the filter vat and fills the filter vat through the siphon principle, and then the water pump is opened, so that circulating filtration can be started.
The filter barrel can simultaneously realize biochemical filtration of biochemical filter materials and physical filtration of filter cotton, realizes one barrel with multiple purposes, does not damage the integral structure of the cylindrical container, can be integrally arranged outside the cylindrical container, and is flexible in configuration. The mode that can also adopt many barrels to establish ties, the outlet pipe of last filter vat communicates the inlet tube of next filter vat promptly, inside last filter vat outlet pipe intercommunication cylindrical container, can infinitely increase filtration space in theory, filtration efficiency is high, low cost.
The filter vat can set up waterproof ultraviolet lamp in, can open as required for eliminate partial plankton in aqueous, the filter vat outer wall adopts the shading material to carry out the light-resistant processing simultaneously, can not lead to the fact the influence to the periphery after opening.
The adhesion test evaluation system comprises a bracket, a motor and a rotating shaft, wherein the bracket is arranged above the cylindrical container, and the motor is arranged on the bracket. The rotation axis sets up at cylinder container the central axis, and the cooperation columniform container can guarantee that the scour state that receives of every sample is identical completely. One end of the rotating shaft is connected with the motor, and the other end of the rotating shaft extends into the cylindrical container.
One of the following settings is used to simulate the dynamic environment and provide sample placement locations:
(1) Install on the rotation axis along with axle pivoted wheel hub, set up the sample cell on the wheel hub side (the utility model provides a wheel hub side indicates cylindrical wheel hub's circumference side). When the device is used, the samples are clamped in the sample grooves on the side faces of the hub and rotate along with the hub, and a plurality of samples, such as 20-30 samples, can be placed at the same time, so that a high-throughput simulation experiment is realized.
The method can simulate the dynamic environment of the ship body material when the ship sails, and further simulate the biofouling condition of the material in the dynamic environment.
(2) The rotating shaft is provided with a hub rotating along with the shaft, and a sample groove is arranged in the side surface of the cylindrical container. When in use, the sample is clamped in the sample groove in a manner similar to a 'cake sticking', water in the container flows along with the rotation of the hub to wash the standing sample, and a plurality of samples can be placed simultaneously.
This way, the dynamic environment of the offshore drilling platform structure material can be simulated.
(3) And a plurality of sample positioning structures are axially arranged along the rotating shaft and used for fixing disc-shaped samples. The sample positioning structure can be a plurality of modes such as a clamping groove and a bolt, and can realize that a plurality of disc-shaped samples are fixed by penetrating into a string by the rotating shaft and rotate along with the rotating shaft. This allows the disc samples to be painted with paint in a manner that simulates biofouling of paint as it is washed at different line speeds.
The illumination system is a full-spectrum LED lamp with adjustable brightness, is arranged above the cylindrical container, and can adapt to illumination requirements of growth of different aquatic organisms or simulate illumination of actual sunrise and sunset through adjustment of wavelength and brightness.
The utility model discloses an experimental apparatus specifically is applied to the cultivation to common easy attached aquatic organism and carries out the simulation aspect of material anti-biofouling performance under the dynamic environment, and the method of adopting above-mentioned experimental apparatus to carry out the experiment includes following step:
s1: collecting water sample and seeding matrix (such as algae stone) attached with aquatic organisms according to the water body environment to be researched, putting the seeding matrix into a cylindrical container, and simultaneously injecting the water sample into a tank; if the overflow hole is arranged, the water level line is required to be kept lower than the overflow opening so as to prevent the water sample from flowing out.
S2: and (3) opening a circulating filtration system, starting a temperature controller and an oxygen supply air pump, and putting a plurality of common aquatic organisms into the cylindrical container after the environment (including water flow velocity, temperature, oxygen content and the like) of the water sample in the cylindrical container is stable. Common aquatic organisms are organisms that survive in the aqueous environment under study and provide nutrients to the ecosystem. For example, if a freshwater body is to be studied, the common aquatic organisms can be grass goldfish, about 1 to 10 pieces of grass goldfish are put in the grass goldfish, and the length of each piece of grass goldfish can be about 3 to 5 cm.
Preferably, the temperature controller controls the environment temperature in the cylindrical container to be 0-35 ℃, and covers most of the water body temperature. In winter, the heating mode can be started to prevent organisms from dying due to low temperature; the cooling mode can be started in summer to prevent the death of organisms due to overhigh temperature. Meanwhile, the user can set the temperature controller to be at the designated temperature, the constant temperature in the cylinder is kept, and the influence of the temperature difference of the external environment is avoided.
S3: the wavelength and the brightness of an illumination system are adjusted to provide illumination for the growth of aquatic organisms, the cylindrical container is placed under the illumination to culture the aquatic organisms, the excrement and the like of common aquatic organisms provide nutrition for an ecological system, and the culture of the aquatic organisms and the introduction of common microorganisms are completed through introduction matrixes such as algae and stones collected in a specific water body environment.
By adjusting the color temperature, the wave band, the illumination intensity, the illumination time and the like of the illumination system lamps, sunrise and sunset can be simulated, for example, 8/16h illumination/dark time is set, or the illumination which is most suitable for the growth of the required organisms can be adjusted to realize the effect of accelerating the cultivation.
S4: the material template to be tested is mounted in a sample tank or on a positioning structure on a rotating shaft (disc-shaped sample), and the required rotating speed is adjusted by a motor according to the three setting modes.
Preferably, the motor can adjust the rotating speed range to be 0-9000rpm, and can simulate different states of ship sailing.
S5: according to the biological adhesion conditions such as the biological adhesion type on the surface of the material sample plate, the adhered area size and the like, the biological fouling prevention capability of the material under the dynamic environment is simulated and evaluated. In addition, the growth condition of the fouling organisms can be judged according to the biological condition attached to the cylinder wall of the cylindrical container and the color of the water body.
The utility model discloses following beneficial effect has:
the utility model provides a can accomplish the cultivation of common aquatic organism in ocean and the fresh water and simulate and survey the device of research with and to the developments antipollution organism adhesive force of different materials under the different water environment, the preparation method is simple, low price, and convenient operation, need not professional power equipment, be applicable to indoor simulation experiment, and can cultivate multiple different common aquatic organism simultaneously, compensate the current indoor experiment and be difficult to reflect natural environment's the stained situation of living beings and the defect that the indoor device is difficult to carry out dynamic simulation, the efficiency of indoor simulation experiment has been improved.
Drawings
Fig. 1 is a perspective schematic view of an apparatus according to an embodiment of the present invention.
Fig. 2 shows a sample arrangement in an apparatus according to an embodiment of the present invention.
Wherein: 1-cylindrical container, 2-oxygen supply air pump, 3-temperature controller, 4-bracket, 5-motor, 6-motor controller, 7-rotating shaft, 8-hub, 9-lamp, 10-water inlet pipe, 11-filtering barrel, 12-water outlet pipe, 13-quick-connection elbow, 14-biochemical filter material clapboard, 15-water inlet grid, 16-grid, 17-water inlet conduit, 18-exhaust valve, 19-seeding matrix, 20-goldfish, 21-sample, 22-thermometer.
Detailed Description
The present invention will be further explained with reference to the drawings and examples. The materials and equipment used in the examples of the present invention are conventional in the art, unless otherwise specified.
As shown in fig. 1, the present embodiment provides a simulation experiment apparatus for simultaneously completing the cultivation of common aquatic organisms and the biological adhesion on the surfaces of different materials in a dynamic environment, which includes a circulating filtration system, an illumination system, a biological cultivation system and an adhesion test evaluation system.
The biological culture system comprises a cylindrical container 1, an oxygen supply air pump 2, a temperature controller 3 and a thermometer 22, wherein the cylindrical container is made of polytetrafluoroethylene materials and can be used for respectively culturing common plankton and periphyton.
Oxygen supply air pump, thermostat, thermometer set up inside the cylinder container, through the temperature of the water temperature in the thermostat control system to the temperature that is most suitable aquatic organism to grow, through thermometer monitoring water temperature, provide oxygen for the biology in the biological culture system through small-size oxygen supply air pump.
The outer wall of the temperature controller and the heating rod are made of high-strength explosion-proof quartz glass, the inner portion of the temperature controller is made of a nickel-chromium alloy resistance wire and an R22 refrigerant, and the temperature controller is internally provided with an HIC constant temperature chip which can control the temperature of the water body more accurately.
The adhesion test evaluation system comprises a bracket 4, a motor 5 and a rotating shaft 7, wherein the bracket is two stainless steel metal long plates arranged above the cylindrical container and is lapped above the cylindrical container through a clamping groove, the motor is placed on the bracket, and a transmission shaft of the motor extends out of a gap between the two stainless steel long plates and is connected with the rotating shaft arranged at the axis position in the cylindrical container. The motor of this embodiment adopts the motor of low friction low vibration, and the complete machine produces the noise and is less than 40dB, and the operation in-process hardly causes harm to people's hearing.
A motor controller 6 is also provided on the carriage, near the motor, for adjusting the rotational speed of the motor.
In FIG. 1, a hub 8 rotating with the shaft is attached to the rotating shaft, and 20 to 30 sample wells are provided on the side surface of the hub. When in use, the sample 21 is clamped in the sample groove on the side surface of the hub and rotates along with the hub, so that the dynamic environment of the ship body material is simulated when the ship sails.
The sample well and sample may also be located inside the side of the cylindrical container, similar to a "pie" format. Under the condition, water in the container flows along with the rotation of the hub during the experiment, the static sample is washed, and the dynamic environment of structures such as an offshore drilling platform is simulated.
The specimen 21 may also be arranged in the manner shown in fig. 2, with a plurality of specimen positioning structures arranged axially along the axis of rotation for holding disc-shaped specimens. The sample positioning structure can be a clamping groove, a bolt and other modes. When a simulation experiment is carried out, a plurality of disc-shaped samples are fixed in a string by penetrating through the rotating shaft and rotate along with the rotating shaft. This allows the disc samples to be painted with paint in a manner that simulates biofouling of paint as it is washed at different line speeds.
The illumination system is a group of full-spectrum LED lamps 9, and can provide 28 kinds of light with different wavelengths and 0-100% of brightness to adapt to illumination requirements of different aquatic organisms for growth. The built-in controller of lamps and lanterns can realize fresh water, biological full coverage formula cultivation of sea water through functions such as cell-phone APP control its illumination intensity, illumination spectrum wave band and time switch, can provide different illumination for different living beings, perhaps the sunrise sunset under indoor simulation true environment.
The circulating filtration system comprises a water inlet pipe 10, a filter barrel 11, a water pump and a water outlet pipe 12, wherein the filter barrel is a cylinder with a cover and a radius of 15cm and a height of 40cm, the top of the filter barrel is provided with a water outlet with a diameter of 2cm, the water outlet is communicated with the water outlet pipe through a quick-connection elbow 13, the bottom of the filter barrel is provided with a water inlet with a diameter of 2cm, and the installation positions of the water inlet and the water outlet are staggered. The water pump is not shown in the figures, but it will be understood by those skilled in the art that the water pump may be placed on the water inlet pipe to effect that water in the cylindrical vessel is drawn into the water inlet pipe and flows back into the cylindrical vessel through the filter basket and the water outlet pipe to effect the circulation filtration.
The lower end of the interior of the filter barrel is provided with a biochemical filter material baffle plate 14, a plurality of water inlet grids 15 are arranged on the biochemical filter material baffle plate, the water inlet grids are separated by a grid 16, and filter cotton is arranged in the water inlet grids. One end of the water inlet conduit 17 is communicated with the water inlet pipe, and the other end of the water inlet conduit enters the bottom of the filter barrel through the water inlet and leads to the bottom of the biochemical filter material partition plate. The water that enters the filter vat is led to biochemical baffle bottom by the water inlet pipe, and the water that enters the filter vat is from supreme down through biochemical filter media baffle and the filtration of the filter pulp on a plurality of water grids that intakes, via the outlet pipe, gets back to in the cylinder container.
When the filter vat and the circulating system are applied, the water inlet pipe is inserted into water, the exhaust valve 18 on the vat cover is opened, the filter vat is vacuumized through the exhaust valve by means of an air pump and the like, water enters the filter vat and fills the filter vat through the siphon principle, and then the water pump is opened, and circulating filtration can be started.
The operation steps of the device are as follows:
s1, firstly, collecting a water sample and stones with periphyton as an introduction substrate 19, putting the stones into a cylindrical container, and injecting the water sample.
In the embodiment, the introduction substrate is a stone to which the aquatic organisms to be evaluated are attached to the water area to be tested, and other introduction substrates from a test coast or a river bank, such as algae stones, stock solution and the like, can also be adopted by the skilled person.
S2, opening the circulating and filtering system, starting a temperature controller and an oxygen supply air pump, after the circulating and filtering system operates for 3-4 hours and the environment of the water sample in the cylindrical container is stable, throwing a plurality of common aquatic organisms which can survive in the water body environment to be researched, namely a grass carp 20 (the grass carp cannot be too small to be sucked by the filtering system) with the length of 3-5cm into the water;
and S3, setting parameters such as switching time, illumination intensity and illumination wave band of the full-spectrum LED lamp according to experimental simulation requirements, providing illumination for growth of aquatic organisms, and placing the cylindrical container under the illumination to culture the aquatic organisms.
And S4, installing the material sample plate to be tested in the sample tank or on a positioning structure on the rotating shaft, wherein the material sample plate is specifically determined according to an arrangement mode. The motor is adjusted to the required rotation speed.
And S5, evaluating the anti-biofouling capacity of the sample plate according to the biological adhesion condition on the surface of the sample plate. In addition, the growth condition of the microorganisms can be judged according to the biological condition attached to the wall of the cylindrical container and the color of the water body.
The utility model discloses compare with traditional indoor experiments, the wave band and the water environment state simulation of different light are adjusted to the accessible, the different waters of reduction, like ocean and the original rivers of river, temperature and illumination decay environment, can also simulate dynamic environment such as steamer navigation simultaneously, realize dynamic simulation effect with higher speed, make the data that obtain more accurate, and can accomplish the cultivation of common plankton and epiphytic simultaneously, simulate the biological stained condition under the natural environment better.
Claims (5)
1. A biofouling dynamic simulation experiment device is characterized by comprising a circulating filtration system, an illumination system, a biological culture system and an adhesion test evaluation system;
the biological culture system comprises a cylindrical container, an oxygen supply air pump, a temperature controller and a thermometer, wherein the oxygen supply air pump, the temperature controller and the thermometer are arranged in the cylindrical container; fresh water or seawater samples are filled in the cylindrical container;
the circulating filtration system comprises a water inlet pipe, a filter barrel, a water pump and a water outlet pipe, wherein filter cotton or/and biochemical filter materials are arranged in the filter barrel, so that water entering the filter barrel can be physically or/and biochemically filtered, the water inlet pipe and the water outlet pipe are communicated with the interior of the filter barrel and the interior of the cylindrical container, and the water pump is arranged on the water inlet pipe;
the adhesion test evaluation system comprises a bracket, a motor and a rotating shaft, wherein the bracket is arranged above the cylindrical container, the motor is arranged on the bracket, the rotating shaft is arranged on the central axis of the cylindrical container, one end of the rotating shaft is connected with the motor, and the other end of the rotating shaft extends into the cylindrical container;
the illumination system is a full-spectrum LED lamp with adjustable brightness and is arranged above the cylindrical container;
the biofouling dynamic simulation experiment apparatus further comprises one of the following three characteristics:
(1) A hub rotating along with the shaft is arranged on the rotating shaft, and a sample groove is arranged on the side surface of the hub;
(2) A hub rotating along with the shaft is arranged on the rotating shaft, and a sample groove is arranged in the side surface of the cylindrical container;
(3) And a plurality of sample positioning structures are arranged along the axial direction of the rotating shaft and used for fixing disc-shaped samples.
2. The biofouling dynamic simulation experiment device of claim 1, wherein the filter drum comprises a water inlet conduit, a biochemical filter material partition plate, a plurality of water inlet cells, a drum cover and an exhaust valve, the biochemical filter material partition plate is arranged at the lower end of the filter drum, one end of the water inlet conduit is communicated with a water inlet pipe, and the other end of the water inlet conduit is communicated with the bottom of the biochemical filter material partition plate;
the plurality of water inlet grids are arranged above the biochemical filter material partition plate in the filter barrel, the water inlet grids are mutually separated by grids, and filter cotton is arranged in the water inlet grids;
set up discharge valve on the bung, discharge valve air inlet and the inside intercommunication of filter vat, the gas outlet intercommunication filter vat is outside.
3. The biofouling dynamic simulation experiment apparatus according to claim 1, wherein the heating device of the temperature controller is a heating rod made of explosion-proof quartz glass.
4. The biofouling dynamic simulation experiment device of claim 1, wherein a waterproof ultraviolet lamp is arranged in the filter tank, and the outer wall of the filter tank is made of a light-shielding material.
5. The biofouling dynamic simulation experiment device of claim 1, wherein the cylindrical vessel contains an seeding matrix and common aquatic organisms.
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| CN202221658934.0U CN218298173U (en) | 2022-06-30 | 2022-06-30 | Biofouling dynamic simulation experiment device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116242966A (en) * | 2023-02-01 | 2023-06-09 | 南通市飞宇石油科技开发有限公司 | Gas drives high pressure fluid phase state analysis appearance |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116242966A (en) * | 2023-02-01 | 2023-06-09 | 南通市飞宇石油科技开发有限公司 | Gas drives high pressure fluid phase state analysis appearance |
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