CN102101725B - Multifunctional lake ecology restoration simulation device and use method as well as application - Google Patents
Multifunctional lake ecology restoration simulation device and use method as well as application Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Hydroponics (AREA)
Abstract
The invention belongs to the technical field of ecological engineering, particularly relating to a multifunctional lake ecology restoration simulation device and use method as well as application. The device comprises a multifunctional ecology restoration simulation test cylinder, a light source control system and a water temperature control system, wherein the light source control system and the water temperature control system are positioned outside the top of the test cylinder; the outer surface of a reaction cylinder is provided with a top cover, a culture liquid inlet and a circulating water outlet; the middle of the reaction cylinder is provided with a water sampling port, and the lower part of the reaction cylinder is provided with the circulating water inlet, a substrate sludge pore water sampling port and a bottom plug; and the test cylinder internally comprises a nitrogen cycle bacteria carrier, submerged plants and settings. By using the method and device, the comprehensive regulation and control as well as optimization of parameters such as settings, submerged plants, denitrification microorganism, water temperature, light and water quality can be carried out, lake and inflowing riverway nutrient salt can be simulated and controlled, and a lake ecology restoration mechanism can be studied. The device disclosed by the invention is simple in structure, is strong in mobility, is diversified in restoration simulation function, is simple in manufacturing, is low in cost and is easy to operate, and can be used indoor and outdoor.
Description
Technical field
The invention belongs to the ecological engineering technology field, be specifically related to method and apparatus and method of application and application that a kind of multi-functional simulation lake ecological is repaired.
Background technology
Quickening along with China's rapid economy development and town and country construction; Along the river the discharging of pollutants such as area, lake nitrogen, phosphorus, organism increases year by year; The pollution becoming serious of lake and stream entering lake water environment; Show as mainly that water area falls sharply, obliteration of lake is serious, lake and river channel ecology system damage, lake eutrophication aggravation, its reason mainly contains: regional industry sewage along the lake, sanitary sewage, agricultural area source discharging are to the pollution of water body in lake; The lake seining cultivation has destroyed lake aquatic attitude system, and the bait of input quickens the eutrophication in lake; Ring lake basin atmospheric pollution dried wet deposition causes the lake Effects of water environment.
Body eutrophication is meant that water body such as lake admits nutritional substances such as excessive nitrogen, phosphorus; Algae and other hydrobionts are bred unusually; Water transparency and dissolved oxygen DO change; Cause the lake water quality deterioration, quicken the lake and wear out, thus the phenomenon that makes lake ecosystem and water function hindered and destroy.Lake eutrophication has become one of limiting factor of restriction social economy sustainable development.
At present, the focus of the research of the eutrophication control of water body in lake mainly concentrates on the control technology to nutritive salt, and for example aquatic higher plant nutritive salt absorbs reparation, immobilized microorganism technique etc.Aquatic higher plant recovery technique adopts hydrophyte to repair more; Comprise (number of patent applications 03139848.0 such as emergent aquactic plant, pleuston, submerged plant; A kind of plant restoration method of eutrophication water); Immobilized microorganism technique utilizes the removal of microorganisms water pollutant (number of patent application 200510038832.3, efficient nitrogen-cycle bacteria artificial screening and the application in Ecological Recovery of Water and water treatment) of enrichment on the carrier etc.
Main pleuston, emergent aquactic plant, and the local endemic species such as submerged plant of adopting in aquatic higher plant recovery technique; Through the nutriment in the water bodys such as a large amount of absorbed nitrogens of the growth metabolism of plant self, phosphorus, absorption suspended particulate substance, inhibition algal grown, enriching heavy metal (number of patent application 200410066162.1, a kind of method of treatig waste water containing chromium with aquatic plant) etc.Adopt the ecological bed technology of submergence type (number of patent application 200910034673.8; The ecological outstanding bed of a kind of submergence type of purifying eutrophication water body) can carry out depth of water adjustment according to transparency and water quality parameter, utilize submerged plant-aquatic microorganisms system synergy in eutrophication water, to remove water nutrition salt.
Now, the lake scientist thinks that the restriction factor that causes body eutrophication mainly is a nitrogen phosphorus, and one of main target of lake ecological reparation is exactly to control lake nutritive salt level, keeps lake clear water stable state.Though many scholars have carried out big quantity research to various lake ecological reparations; But present research major side overweights lake treatment effect aspect; Less relatively for the research of restoration of the ecosystem mechanism; How the optimal combination different ecological is repaired means just can make lake denitrogenation dephosphorizing maximized, still indeterminate at present.On the other hand, the method and apparatus that can simulate the restoration of the ecosystem of different lake environment condition, the different lake ecologicals reparation of contrast means treatment effect, discussion restoration of the ecosystem mechanism still remains to be developed, and has hindered the progress of lake ecological repair mechanisms.
Actually rare for the method and apparatus of lake ecological reparation simulation at present.There is the scholar to propose a kind of Wetland micro-ecological simulation method; Be intended to the wetland bed mud sample of field acquisition is placed on formation wetland post in the PVC hydrostatic column respectively; Realize the simulation wetlands ecosystems are carried out temperature range control and detected (number of patent application 200910098001.3, a kind of Wetland micro-ecological simulation method) through temperature control device then; Also there is the scholar to research and develop equipment simulating lake sediment-water and soil interface environments, artificially controls interfacial water energetic disturbance, interface illumination, interface temperature and overlying water chemical characteristic, research water and soil interface biochemical process mechanism.(number of patent application 200710133406.7, a kind of indoor simulated system that is applied to lake sediment-water interface process research).
Though Chinese scholars has been carried out certain research to water body in lake eutrophication restoration of the ecosystem simulation; And a series of technology have been researched and developed; But up to the present, can factors such as original position lake sediment, overlying water, submerged plant, nitrogen cycle bacterium be incorporated into the multifunctional ecological reparation analogue technique method of studying in the system does not also appear in the newspapers.Adopt manual simulation's means of different lake ecological repair process; Comprise comprehensive regulation and optimizations such as sediment, submerged plant, denitrification microorganism, water temperature, illumination and water quality parameter, the multi-functional analogue means of control lake nutritive salt, research lake ecological repair mechanisms does not have exploitation as yet.Therefore, improvement has positive effect to nutrition-enriched water of lake with device to study this type of technology.
Summary of the invention
The technical issues that need to address of the present invention are to be difficult to simulate different lake ecologicals reparation means to serious further lake eutrophication problem and existing apparatus; The present invention provides a kind of method and apparatus that different lake ecologicals are repaired means of simulating; Can effectively simulate different lake ecologicals and repair means, thereby can promote the research of original position lake ecological repair mechanisms.Use can the manual simulation different lake ecological repairing environments of this device; Regulation and control physics, chemistry, biodyne; Study of the influence of each factor, promote various combination restoration of the ecosystem means control lake nutritive salt, improve the research of water body in lake ecological environmental condition water body in lake restoration of the ecosystem, denitrogenation dephosphorizing.
It is a kind of cylindrical transparent unit that multi-functional lake ecological according to the invention is repaired analogue means; Every separated certain distance is provided with sample tap; Repair the requirement of means gathers the original position bed mud, adds denitrification microorganism, hydrophyte, a series of environmental impact factors of regulation and control water body: water nutrition salinity, temperature, light intensity according to different lake ecologicals; The lake or the river bottom mud sediment of this analogue means bottom filling in-situ acquisition; Add the restoration of the ecosystem of different aquatic plant simulation lacustrine plant; Add the restoration of the ecosystem of different proportioning hydrophyte simulation lacustrine plant; Utilize the microbe carrier of pore mesh bag parcel in cylinder, to add the reparation of denitrification microorganism simulation microbial ecological; Add plant and denitrification microorganism simulating plant-microorganism coupling restoration of the ecosystem; Also can be through by adding hot water storgae, built-in temperature temp probe and outreaching the temperature control system regulation and control water temperature that temperature controller forms; Through being furnished with the halogen light source regulating system intensity of illumination of light intensity control, simulate different water body in lake environmental baselines; At this device differing heights the sample tap sampling is set, through measuring water body and pore water nutritive salt, pH, ORP, DO, N
2, bed mud denitrifying bacterium quantity physical and chemical index, research water nutrition salt is removed ability and denitrogenation speed, thus effectively simulation and the different lake ecologicals of comparative study are repaired means.The device accompanying drawing is seen Figure 16.
Technical scheme of the present invention:
A kind of device of simulating the lake ecological reparation; Light source outside comprising testing column and being positioned at the testing column top, with testing column the hot water storgae that adds that water pipe is connected is arranged, the testing column top is a transparency cover, and the testing column cartridge outer surface down sets gradually nutrient solution import, circulating water outlet from top; The centre is provided with the water quality sample tap; The bottom is provided with the import of recirculated water, sediment pore water sample tap, and the bottom is provided with movable bottom plug.Alternative successively from top to bottom immobilization nitrogen cycle bacterium, submerged plant, planktonic organism, the bed mud placed in testing column inside.
Said top cover and bottom seals plug are all detachable.Add temp probe is housed in the hot water storgae, temp probe is associated with temperature controller outward.Light source is equipped with light intensity control, can regulate the control light intensity.The outer nutrient solution storage tank of reaction column connects with reaction column through the nutrient solution import.
Intensity of illumination control: can select natural lighting or artificial lighting dual mode, when the testing column device adopts halogen light source 8 irradiations,, adopt light intensity control 7 to regulate light source control intensity of illumination, light intensity regulating scope 0Lux ~ 10000Lux with light intensity instrumentation amount; The original position intensity of illumination is logical can cross automatic weather station monitoring acquisition experimental data.
Temperature control:, testing column adopts constant temperature jacket to control temperature by temperature controller 3, and the control culture-liquid temp is at 10～45 ± 1 ℃, and in-situ temperature can obtain data through the automatic weather station monitoring.
Nutritive salt control: according to original position aquatic monitoring water quality parameter; The nutrient solution of configuration simulation lake, river water quality; Selectable through 121 ℃ of high-temperature sterilization 30min of pressure cooker, pass through supercharge pump according to certain flow by the nutrient solution storage tank, through nutrient solution inlet injection test post.
Immobilized microorganism is thrown in: discharge four kinds of indigenous nitrogen cycle bacterium (amonifying bacteria, nitrite bacteria, nitrobacteria, and denitrifying bacteria) through fixed microorganism carrier; With pore mesh bag coated carrier; Carrier weight in wet base 50g/ bag; Be fixed in top cover for one section through fine rule, carrier is hung on testing column top water body, the microbe carrier replacing time is a week.
A kind of method of application of multi-functional simulation lake ecological prosthetic device:
(1) nutrient solution and testing column sterilization: the nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of UV radiation method;
(2) adopt columnar sampler to gather original position bed mud sediment; The bottom mud in lake that to take from original position is packed into the column bottom from the testing column bottom, and thickness reaches 20cm, is adjusted to proper height; The water and soil interface is flushed with first pore water sample tap, and the outer parcel aluminium-foil paper of surveying makes the bed mud side be the shading state;
(3) select the healthy and strong submerged plant of certain biomass growing way, the about 10cm of every strain submerged plant length evenly is planted on the bed mud;
(4) with sterilization back nutrient solution in 1, inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level through pump;
(5) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and sediment with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(6) under restoration of the ecosystem means of setting and environmental baseline, each factor in the lake ecological reparation simulation post is carried out observation analysis, regularly pH, ORP, DO, TP, TN, the NH in the test simulator water body
4 +, NO
3 -, NO
2 -, dissolubility N
2Deng, analyzing water body nutritive salt removal effect and restoration of the ecosystem effect;
In the step (3) submerged plant can according to different lakes submerged vegetation feature selecting typical case submerged plant waterweed (
Elodea nuttallii), black algae (
Hydrilla verticilla), eel grass (
Vallisneria natans), little tooth bog pondweed (
P.maackianus).
The invention has the beneficial effects as follows provides a kind of method and apparatus that the lake different ecological is repaired means of simulating, and combines reparation means such as bed mud sediment denitrification, submerged plant absorption, the effect of denitrogenation circulation bacterium.Apparatus of the present invention can be simulated different lake ecologicals and repair means; Like means such as plant, microorganism, plant-microorganism couplings; Control multiple physics, chemistry, biodyne, like temperature, intensity of illumination, concentration of nitrogen and phosphorus, submerged plant, nitrogen cycle bacteria biomass etc., the experiment contrast effect is obvious; Can effectively simulate of the influence of various environmental factors, thereby theoretical foundation and technical support are provided for the lake ecological reparation the lake ecological repair mechanisms.
Different ecological reparation means that apparatus of the present invention are integrated; Both can investigate clean-up effect and the mechanism of single method to water body in lake; Also can contrast between the distinct methods; Perhaps the treatment effeciency during the above synergy of two kinds of methods is studied each factor removes ability to lake ecological reparation, nitrogen phosphorus influence size.
Apparatus of the present invention are simple in structure, and cost is low, and are easy to operate, easy to make, and diverse in function can be carried out the simulation comparative study that different ecological is repaired means easily.Therefore, this invention is the efficient apparatus of simulation eutrophic lake restoration of the ecosystem means, the ecological repair mechanisms of research.
Description of drawings
Fig. 1, Fig. 2, Fig. 3, Fig. 4. embodiment 1 lab scale test nitrogen nutrition salt is changing trend diagram in time;-■-ammonia nitrogen concentration (mg/L);-●-nitrate nitrogen concentration (mg/L) ,-▲-nitrite nitrogen concentration (mg/L) ,-▼-total nitrogen concentration (mg/L);
Fig. 5. embodiment 2 lab scales test denitrifying bacterium quantity is variation tendency in time ,-■-sediment post (* 10
5Cells/g) ,-●-sediment+waterweed post (* 10
5Cells/g) ,-▲-sediment+waterweed+microorganism (* 10
5Cells/g);
Fig. 6. embodiment 3 field test condition of different temperatures influence rule, 1#:10 ℃, 2#:18 ℃, 3#:25 ℃, 4#:35 ℃ to plant-microorganism coupling restoration of the ecosystem denitrogenation speed;
Fig. 7. embodiment 4 field test different ecologicals are repaired water body dissolved oxygen DO variation tendency under the means condition ,-■-sediment post (umol/L) ,-●-sediment+black algae post (umol/L) ,-▲-sediment+black algae+nitrogen cycle bacterial spawn (umol/L);
Fig. 8. embodiment 4 field test different ecologicals are repaired water body pH variation tendency under the means condition ,-■-sediment post ,-●-sediment+black algae post ,-▲-sediment+black algae+nitrogen cycle bacterial spawn;
Fig. 9. four kinds of restoration of the ecosystem submerged plants of embodiment 5 shop experiments TN clearance ,-■-eel grass (mg/L) ,-●-waterweed (mg/L) ,-▲-little tooth bog pondweed (mg/L) ,-▼-black algae (mg/L);
Simulation test plant-microorganism coupling nutritive salt variation tendency is in time repaired in Figure 10: embodiment 6 river courses ,-■-total nitrogen concentration (mg/L) ,-●-total phosphorus concentration (mg/L) ,-▲-ammonia nitrogen concentration (mg/L) ,-▼-COD (mg/L)
Waterweed post water body dissolved oxygen concentration under Figure 11: the embodiment 7 shop experiment different illumination conditions ,-■-2000Lux (umol/L) ,-●-3000Lux (umol/L) ,-▲-4000Lux (umol/L) ,-▼-6000Lux (umol/L);
Figure 12. the different lake region sediment nitrogen cycle bacterium propagation of embodiment 8 field tests trend ,-■-Mei Liang gulf dredged area (* 10
5Cells/g) ,-●-tribute arm of lake (* 10
5Cells/g) ,-▲-river course (* 10
5Cells/g) ,-▼-eastern Taihu Lake (* 10
5Cells/g);
Figure 13. embodiment 9 shop experiment submerged plants are repaired mesoporosity water TN and change ,-■-black algae post (mg/g dry weight) ,-●-control samples (mg/g dry weight);
Figure 14. embodiment 10 field test plant-microorganism couplings are to different quality ammonia nitrogen removal effect ,-■-1# (mg/L) ,-●-2# (mg/L) ,-▲-3# (mg/L) ,-▼-4# (mg/L);
Figure 15. embodiment 10 field test plant-microorganism couplings are to different quality nitre nitrogen removal effect ,-■-1# (mg/L) ,-●-2# (mg/L) ,-▲-3# (mg/L) ,-▼-4# (mg/L);
Figure 16. multifunctional ecological is repaired the analogue means structural representation, adds hot water storgae 1, temp probe 2, temperature controller 3, supercharge pump 4; Circulating water intake 5, circulating water outlet 6, light intensity control 7, halogen light source 8, nutrient solution import 9; Denitrification microorganism carrier 10, testing column 11, water body sample tap 12, submerged plant (waterweed) 13, bed mud pore water sample tap 14; Sediment 15, top cover 16 seals film 17, nutrient solution storage tank 18, bottom plug 19.
Embodiment
Below in conjunction with accompanying drawing and embodiment the present invention is further specified:
Indoor lab scale (investigate sediment, plant, microorganism in lake ecological is repaired to the nutritive salt removal effect)
The device of simulated ecological reparation comprises supercharge pump 4, nutrient solution import 9, and denitrification microorganism carrier 10, testing column 11, water body sample tap 12, submerged plant (waterweed) 13, sediment 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Because this lab scale uses natural lighting and only need satisfy the condition of different tests post as room temperature, add hot water storgae 1, temp probe 2, temperature controller 3, circulating water intake 5, circulating water outlet 6, light intensity control 7, halogen light source 8 and be not provided with at this embodiment.Testing column 11 cartridge outer surface sample taps 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Denitrification microorganism carrier weight in wet base 50g, it is inner from liquid level 10cm place to be fixed on testing column with the pore mesh bag.
The nutrient solution preparation adds with ammonium sulfate and sodium nitrate, makes that ammonia nitrogen concentration and the nitrate nitrogen concentration in the nutrient solution reaches respectively about 2.0mg/L and 1.0mg/L, and in nutrient solution, replenishes proper amount of carbon source, simulation eutrophic pollution water quality.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of UV radiation method;
(3) will take from the bottom mud in lake of original position, be packed into the column bottom by original appearance, be adjusted to proper height, the water and soil interface is flushed with first pore water sample tap from the testing column bottom; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(4) choose the healthy and strong waterweed of certain biomass growing way, the about 10cm of every strain submerged plant length evenly is planted on the bed mud;
(5) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level through pump;
(6) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and sediment with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(7) each factor in the lake ecological reparation simulation post is carried out observation analysis, regularly pH, ORP, DO, the NH in the test simulator water body
4 +, NO
3 -, NO
2 -Deng water quality parameter, analyzing water body nutritive salt removal effect and restoration of the ecosystem effect;
1# testing column (only adding sediment) step: (2), (3), (5), (7)
2# testing column (sediment+waterweed) step: (2), (3), (4), (5), (7)
3# testing column (sediment+microorganism) step: (1), (2), (3), (5), (6), (7)
4# testing column (sediment+microorganism+waterweed) step: (1), (2), (3), (4), (5), (6), (7)
In advance incubation time is one month, and microbe carrier is changed with the nutrient solution replacement cycle and is a week, and viewing test post implants growing state treats that root system of plant grows up to, after microorganism fully is diffused in water body and the sediment, cultivation end in advance.More renew nutrient solution then, in the nutrient solution storage tank from the water inlet every day water sampling, detect wherein TN, NH
4 +-N, NO
2 --N, NO
3 -Water-quality guideline such as-N, experimental result such as Fig. 1, Fig. 2, Fig. 3, shown in Figure 4.Behind the 8d, four kinds of different ecological reparation means (corresponding 1#, 2#, 3#, 4# restoration of the ecosystem testing column) are respectively the clearance of ammonia nitrogen, nitrate nitrogen, total nitrogen:
1# testing column (only adding sediment): 27.4%, 41.8%, 32.2%.
2# testing column (sediment+waterweed): 89.5%, 79.6%, 87.1%.
3# testing column (sediment+nitrogen cycle bacterium): 72.3%, 79.1 % 83.3%.
4# testing column (sediment+microorganism+waterweed): 93.2%, 81.4%, 89.1%.
Can find out that by experimental result when adopting submerged plant and the coupling of nitrogen cycle bacterium to repair means, water nutrition salt is removed most effective.Contrast only exists the testing column of lake sediment not good to the removal efficient of water nutrition salt.
Four kinds of nitrogen cycle bacterium of table 1. enrichment culture based formulas
| Chemical constitution (analyzing pure) | Four kinds of bacterium combinations |
| C 6H 12O 6 | 1g |
| (NH4) 2SO 4 | 0.44g |
| KNO 3 | 0.5g |
| NaNO 2 | 0.2g |
| KH 2PO 4 | 0.25g |
| MgSO 4 | 0.02g |
| NaCl | 0.02g |
| FeCl 3 | 0.02g |
| CoCl 2 | 0.002g |
| H 2O | 1L |
Indoor lab scale (investigate different lake ecologicals and repair the influence of means) to sediment denitrifying bacterium quantity
The device of simulated ecological reparation comprises and adds hot water storgae 1, temp probe 2, temperature controller 3, supercharge pump 4; Circulating water intake 5, circulating water outlet 6, light intensity control 7, halogen light source 8; Nutrient solution import 9, denitrification microorganism carrier 10, testing column 11, water body sample tap 12; Submerged plant (waterweed) 13, sediment 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface sample taps 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Denitrification microorganism carrier weight in wet base 50g, it is inner from liquid level 10cm place to be fixed on testing column with the pore mesh bag.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of UV radiation method;
(3) will take from the bottom mud in lake of original position, be packed into the column bottom by original appearance, be adjusted to proper height, the water and soil interface is flushed with first pore water sample tap from the testing column bottom; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(4) choose the healthy and strong waterweed of certain biomass growing way, the about 10cm of every strain submerged plant length is planted on the bed mud uniformly;
(5) with sterilization back nutrient solution in 1, inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level through pump;
(6) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and sediment with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(7) restoration of the ecosystem simulation test column device is put under the indoor environmental condition that sets, light source adopts Halogen lamp LED 8, photoperiod 12h/12h; Intensity of illumination 4000Lux (by light intensity control 7 controls); 20 ± 1 ℃ of temperature (the chuck water bath with thermostatic control is by temperature controller 3 controls).Testing column was carried out for three weeks to be cultivated in advance.
(8) after cultivation finishes in advance, get sediment once in a week, adopt the MPN method to measure the quantity of denitrifying bacterium in the sediment, MPN method denitrifying bacterium nutrient culture media adopts the Giltay nutrient culture media, sees table 2.
1# testing column (only adding sediment) step: (2), (3), (5), (7), (8)
2# testing column (sediment+waterweed) step: (2), (3), (4), (5), (7), (8)
3# testing column (sediment+microorganism+waterweed) step: (1), (2), (3), (4), (5), (6), (7), (8)
This off-test of back around the sampling, experimental result is seen Fig. 5, along with the release of nitrogen cycle bacterium in carrying out of cultivating and the carrier, and compares in the bed mud that does not add the nitrogen cycle bacterium, denitrifying bacterium quantity increases obviously in the bed mud after the interpolation.
Field test (investigate temperature factor lake ecological is repaired the influence of denitrogenation speed)
The device of simulated ecological reparation comprises and adds hot water storgae 1, temp probe 2, temperature controller 3, supercharge pump 4; Circulating water intake 5, circulating water outlet 6, nutrient solution import 9, denitrification microorganism carrier 10; Testing column 11, water body sample tap 12, submerged plant (eel grass) 13; Sediment 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface sample taps 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Natural lighting is adopted in the Yin Ben experiment, so light intensity control 7, halogen light source 8 is not provided with.
The experimental technique step:
(1) nutrient solution and testing column sterilization: the Mei Liangwan in-situ acquisition capacity lake water in Taihu Lake, as restoration of the ecosystem simulation test post nutrient solution, inject the nutrient solution storage tank;
(2) will take from the bottom mud in lake of original position, and be packed into the column bottom from the testing column bottom by original appearance, bed mud thickness is 20cm, is adjusted to proper height, and the water and soil interface is flushed with first pore water sample tap; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(3) choose the healthy and strong eel grass of certain biomass growing way, the about 15cm of every strain submerged plant length is planted on the bed mud uniformly;
(4) with nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level through pump;
(5) restoration of the ecosystem simulation test column device is put under the outdoor environment natural lighting condition that sets, outdoor natural light is about 3900Lux-4100Lux, about 10h/14h of photoperiod according to intensity; Temperature is divided into 15 ℃, 35 ℃ of two gradients (the chuck water bath with thermostatic control is by temperature controller 3 controls), and the outdoor temperature average is 25 ℃.Testing column was carried out for three weeks to be cultivated in advance.
(6) each factor in the lake ecological reparation simulation post is carried out observation analysis, cultivate in advance and finish back DO, the NH in the test simulator water body regularly
4 +, NO
3 -, NO
2 -, the TN water quality parameter, analyzing water body denitrification rate and restoration of the ecosystem effect;
1#, 2#, 3#, 4# testing column temperature are set to 10 ℃, 18 ℃, 25 ℃, 35 ℃ respectively.
Water sampling detected DO, NH in the water body in continuous five days
4 +, NO
3 -, NO
2 -, water quality parameter such as TN, utilize the nitrogen balancing method to calculate water and soil interface denitrification rate in the testing column, experimental result such as Fig. 6 at identical illumination condition, under the condition of different temperatures, can find out in the time of 25 ℃, system's denitrification rate is the highest.
Embodiment 4
Field test (investigating the variation tendency of water quality parameter pH, ORP etc. under the different lake ecological repairing conditions)
The device of simulated ecological reparation comprises supercharge pump 4, circulating water intake 5, circulating water outlet 6, nutrient solution import 9; Denitrification microorganism carrier 10, testing column 11, water body sample tap 12, submerged plant (black algae) 13; Sediment 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface sample taps 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Natural lighting is adopted in the Yin Ben experiment, and not to temperature adjusting, so add hot water storgae 1, temp probe 2, temperature controller 3, light intensity control 7, halogen light source 8 is not provided with.Denitrification microorganism carrier weight in wet base 50g, it is inner from liquid level 10cm place to be fixed on testing column with the pore mesh bag.
The nutrient solution preparation adds with ammonium sulfate and sodium nitrate, makes that ammonia nitrogen concentration and the nitrate nitrogen concentration in the nutrient solution reaches respectively about 2.0mg/L and 1.0mg/L, and in nutrient solution, replenishes proper amount of carbon source, simulation eutrophic pollution water quality.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of UV radiation method;
(3) will take from the bottom mud in lake of original position, and be packed into the column bottom from the testing column bottom by original appearance, thickness is 20cm, is adjusted to proper height, and the water and soil interface is flushed with first pore water sample tap; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(4) choose the healthy and strong black algae of certain biomass growing way in position, the about 10cm of every strain submerged plant length is planted on the bed mud uniformly;
(5) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level through pump;
(6) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and sediment with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(7) each factor in the lake ecological reparation simulation post is carried out observation analysis, regularly the pH in the test simulator water body, ORP, DO,
-Deng water quality parameter, analyze different ecological and repair the influence of means the water quality parameter;
1# testing column (only adding sediment) step: (2), (3), (5), (7)
2# testing column (sediment+waterweed) step: (2), (3), (4), (5), (7)
3# testing column (sediment+microorganism+waterweed) step: (1), (2), (3), (4), (5), (6), (7)
In advance incubation time is one month, and microbe carrier is changed with the nutrient solution replacement cycle and is a week, and viewing test post implants growing state treats that root system of plant grows up to, after microorganism fully is diffused in water body and the sediment, cultivation end in advance.More renew nutrient solution then, in the nutrient solution storage tank, from water inlet, per hour get water sample one time in 10 hours, detect water quality parameters such as wherein DO, pH.Experimental result such as Fig. 7, shown in Figure 8.Can find out that when utilizing submerged plant to carry out the lake ecological reparation, because light of submerged plant and effect release of oxygen, DO concentration will be apparently higher than the water body that does not adopt submerged plant to repair in the water.When using phytoremediation separately, water body pH raises very fast, and pH drops to about 9 after continuing to add microorganism.
Shop experiment (investigate in the different submerged plant lake ecologicals reparations nutritive salt is removed ability)
The device of simulated ecological reparation comprises, supercharge pump 4, circulating water intake 5, circulating water outlet 6; Light intensity control 7, halogen light source 8, nutrient solution import 9, denitrification microorganism carrier 10; Testing column 11, water body sample tap 12, submerged plant (eel grass) 13; Sediment 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface sample taps 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Incubated at room temperature is adopted in the Yin Ben experiment, thus add hot water storgae 1, temp probe 2, temperature controller 3 is not provided with.
The nutrient solution preparation adds with ammonium sulfate and sodium nitrate, makes that ammonia nitrogen concentration and the nitrate nitrogen concentration in the nutrient solution reaches respectively about 2.0mg/L and 1.0mg/L, and in nutrient solution, replenishes proper amount of carbon source, simulation eutrophic pollution water quality.
The experimental technique step:
(1) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of UV radiation method;
(2) will take from the bottom mud in lake of original position, and be packed into the column bottom from the testing column bottom by original appearance, bed mud thickness is 20cm, is adjusted to proper height, and the water and soil interface is flushed with first pore water sample tap, and outer the survey wrapped up aluminium-foil paper and made the bed mud side be the shading state;
(3) choose the healthy and strong eel grass of certain biomass growing way, waterweed, little tooth bog pondweed, black algae, the about 10cm of every strain submerged plant length is planted in different submerged plants on some cultivation post bed muds respectively uniformly;
(4) with nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level through pump;
(5) restoration of the ecosystem simulation test column device is put under the indoor conditions that sets light intensity 4000Lux, about 12h/12h of photoperiod; Temperature is a room temperature.Testing column is carried out cultivating in advance in one month.
(6) each factor in the lake ecological reparation simulation post is carried out observation analysis, cultivate in advance and finish back DO, the NH in the test simulator water body regularly
4 +, NO
3 -, NO
2 -, the TN water quality parameter, analyzing water body denitrification rate and restoration of the ecosystem effect;
Cultivate in advance and more renew nutrient solution after finishing, in the nutrient solution storage tank from the water inlet every day water sampling, detect wherein TN, NH
4 +-N, NO
2 --N, NO
3 -Water-quality guideline such as-N, experimental result is as shown in Figure 9.Behind the 8d, four kinds of submerged plants (corresponding 1#, 2#, 3#, 4# restoration of the ecosystem testing column) are respectively the clearance of total nitrogen:
1# testing column (eel grass): 52.5%
2# testing column (waterweed): 88.7%
3# testing column (little tooth bog pondweed): 66.3 %.
4# testing column (black algae): 82.9%.
Simulation test (investigating plant, the reparation of microorganism coupling river channel ecology to nutritive salt removal efficient) is repaired in the river course
The device of simulated ecological reparation comprises supercharge pump 4, nutrient solution import 9, and denitrification microorganism carrier 10, testing column 11, water body sample tap 12, submerged plant (waterweed) 13, sediment 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Because this lab scale uses natural lighting and only need satisfy the condition of different tests post as room temperature, add hot water storgae 1, temp probe 2, temperature controller 3, circulating water intake 5, circulating water outlet 6, light intensity control 7, halogen light source 8 and be not provided with at this embodiment.Testing column 11 cartridge outer surface sample taps 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Denitrification microorganism carrier weight in wet base 50g, it is inner from liquid level 10cm place to be fixed on testing column with the pore mesh bag.
The nutrient solution preparation adds with ammonium sulfate and sodium nitrate, makes that ammonia nitrogen concentration and the nitrate nitrogen concentration in the nutrient solution reaches respectively about 4mg/L and 2mg/L, simulation heavily contaminated river course eutrophic pollution water quality.In nutrient solution, add glucose simultaneously to replenish proper amount of carbon source.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of UV radiation method;
(3) will take from the heavily contaminated river bottom mud of original position, and be packed into the column bottom from the testing column bottom by original appearance, and make thickness reach 20cm, the adjustment proper height makes the water and soil interface flush with first pore water sample tap; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(4) choose the healthy and strong waterweed of certain biomass growing way in the river course, the about 10cm of every strain submerged plant length is planted on the bed mud uniformly;
(5) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level through pump;
(6) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and sediment with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(7) each factor in the river channel ecology reparation simulation post is carried out observation analysis, regularly TN, TP, DO, the NH in the test simulator water body
4 +, NO
3 -, water quality parameter such as COD, analyzing water body nutritive salt removal effect and restoration of the ecosystem effect;
In advance incubation time is one month, and microbe carrier is changed with the nutrient solution replacement cycle and is a week, and viewing test post implants growing state treats that root system of plant grows up to, after microorganism fully is diffused in water body and the sediment, cultivation end in advance.More renew nutrient solution then, in the nutrient solution storage tank from the water inlet every day water sampling, detect wherein TN, NH
4 +-N, NO
3 -Water-quality guideline such as N, TP, COD.Behind the 10d, plant, microorganism coupling restoration of the ecosystem means are respectively the clearance of the total nitrogen of simulation river course waste water, total phosphorus, ammonia nitrogen, COD: 71.8%, 82.5%, 70.3%, 76.6%.Experimental result is shown in figure 10.
Embodiment 7
Shop experiment (investigating the variation of different light intensity condition cascading water plant oxygen release ability)
The device of simulated ecological reparation comprises, supercharge pump 4, circulating water intake 5, circulating water outlet 6, light intensity control 7; Halogen light source 8, nutrient solution import 9, testing column 11, water body sample tap 12; Submerged plant (eel grass) 13, sediment 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface sample taps 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Incubated at room temperature is adopted in the Yin Ben experiment, and does not add microorganism, thus add hot water storgae 1, temp probe 2, temperature controller 3, denitrification microorganism carrier 10 is not provided with.
The experimental technique step:
(1) nutrient solution and testing column sterilization: the in-situ acquisition capacity lake water in Taihu Lake, as restoration of the ecosystem simulation test post nutrient solution, inject the nutrient solution storage tank;
(2) will take from the bottom mud in lake of original position, and be packed into the column bottom from the testing column bottom by original appearance, bed mud thickness is 20cm, is adjusted to proper height, and the water and soil interface is flushed with first pore water sample tap; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(3) choose the healthy and strong waterweed of certain biomass growing way, the about 10cm of every strain submerged plant length is planted in different submerged plants on some cultivation post bed muds respectively uniformly;
(4) with nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level through pump;
(5) restoration of the ecosystem simulation test column device is put under the indoor conditions that sets, the light intensity gradient is 2000Lux, 3000Lux, 4000Lux, 6000Lux, about 12h/12h of photoperiod; Temperature is a room temperature.Testing column is carried out a week to be cultivated in advance.
(6) each factor in the lake ecological reparation simulation post is carried out observation analysis, cultivate in advance and finish the back, the DO in per two hours test simulator water bodys, 10 off-tests as a child in 5 beginnings in morning;
Experimental result is shown in figure 11.Can find out that in the range of light intensity of 2000 ~ 6000Lux the oxygen release ability of waterweed in restoration of the ecosystem strengthens gradually.
Field test (investigate different lake regions and stream entering lake sediment and throw in nitrogen cycle bacterium cultivation effect)
The device of simulated ecological reparation comprises supercharge pump 4, circulating water intake 5, and circulating water outlet 6, nutrient solution import 9, denitrification microorganism carrier 10, testing column 11, water body sample tap 12,, sediment 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface sample taps 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Natural lighting is adopted in the Yin Ben experiment, and not to temperature adjusting, does not adopt submerged plant, thus add hot water storgae 1, temp probe 2, temperature controller 3, light intensity control 7, halogen light source 8, submerged plant 13 is not provided with.Denitrification microorganism carrier weight in wet base 50g, it is inner from liquid level 10cm place to be fixed on testing column with the pore mesh bag.
The nutrient solution preparation adds with ammonium sulfate and sodium nitrate, makes that ammonia nitrogen concentration and the nitrate nitrogen concentration in the nutrient solution reaches respectively about 2.0mg/L and 1.0mg/L, and in nutrient solution, replenishes proper amount of carbon source, simulation eutrophic pollution water quality.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of UV radiation method;
(3) will take from Mei Liang gulf and the bed mud in river course after tribute arm of lake, eastern Taihu Lake, the dredging; Remove root system of plant, be packed into the column bottom from the testing column bottom by original appearance, thickness is 20cm; Be adjusted to proper height, the water and soil interface is flushed with first pore water sample tap; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(5) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level through pump;
(6) fixation support with four kinds of nitrogen cycle bacterium of enrichment wraps up with the pore mesh bag, and the form of fine rule suspension is thrown in to water body liberating nitrogen circulation bacterium in water body and sediment.The carrier replacement cycle is a week;
(7) each factor in the lake ecological reparation simulation post is observed, regularly the denitrifying bacterium number change in the test simulator bed mud;
The 1# testing column adds tribute arm of lake sediment,
The 2# testing column adds eastern Taihu Lake Sediment
The 3# testing column adds dredging back plum beam bay sediment
The 4# testing column adds river drift
Incubation time is one month, and microbe carrier is changed and the nutrient solution replacement cycle is a week, and jede Woche adopts MPN to measure.Experimental result is shown in figure 12.Can find out that from experimental result behind the input nitrogen cycle bacterium, denitrifying bacterium quantity propagation is the fastest in the eastern Taihu Lake Sediment, and Mei Liang gulf, dredging back bed mud propagation is the slowest.
Shop experiment (investigating in the submerged plant restoration of the ecosystem influence) to pore water nutritive salt
The device of simulated ecological reparation comprises, supercharge pump 4, circulating water intake 5, circulating water outlet 6, light intensity control 7; Halogen light source 8, nutrient solution import 9,, testing column 11; Submerged plant (black algae) 13, pore water sample tap 14 sediments 15 seal film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface sample taps 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Incubated at room temperature is adopted in the Yin Ben experiment, thus add hot water storgae 1, temp probe 2, temperature controller 3, denitrification microorganism carrier 10 is not provided with.
The experimental technique step:
(1) the in-situ acquisition capacity lake water in Taihu Lake as restoration of the ecosystem simulation test post nutrient solution, injects the nutrient solution storage tank;
(2) will take from the bottom mud in lake of original position, and be packed into the column bottom from the testing column bottom by original appearance, bed mud thickness is 20cm, is adjusted to proper height, and the water and soil interface is flushed with first pore water sample tap, and outer the survey wrapped up aluminium-foil paper and made the bed mud side be the shading state;
(3) choose the healthy and strong black algae of certain biomass growing way, the about 10cm of every strain submerged plant length is planted in different submerged plants on the bed mud uniformly;
(4) with nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level through pump;
(5) restoration of the ecosystem simulation test column device is put under the indoor conditions that sets light intensity 4000Lux, about 12h/12h of photoperiod; Temperature is a room temperature.Testing column is carried out cultivating in advance in one month.
(6) each factor in the lake ecological reparation simulation post is carried out observation analysis, cultivate the TN, the NH that finish in the test simulator sediment of back in advance
4 +, NO
3 -, NO
2 -Deng water quality parameter;
The 1# testing column adds black algae, and the 2# testing column is as control samples, and experimental result is shown in figure 13.Can find out that by experimental result behind the interpolation plant, the pore water TN in the shallow-layer bed mud has certain minimizing, and the following bed mud pore water of 5cm total nitrogen there is not significant change.
Field test (investigate plant, microorganism coupling and under the condition of water quality of different lakes, nutritive salt is removed efficient)
The device of simulated ecological reparation comprises supercharge pump 4, light intensity control 7, halogen light source 8, nutrient solution import 9, denitrification microorganism carrier 10; Testing column 11, water body sample tap 12, submerged plant (waterweed) 13; Sediment 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Because this lab scale uses natural lighting, adds hot water storgae 1, temp probe 2, temperature controller 3, circulating water intake 5, circulating water outlet 6, is not provided with at this embodiment.Testing column 11 cartridge outer surface sample taps 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Denitrification microorganism carrier weight in wet base 50g, it is inner from liquid level 10cm place to be fixed on testing column with the pore mesh bag.
The nutrient solution preparation adds with ammonium sulfate and sodium nitrate, makes that ammonia nitrogen concentration and the nitrate nitrogen concentration in the nutrient solution reaches respectively:
About 1# 1mg/L and 0.5mg/L,
About 2# 2mg/L and 1mg/L
About 3# 4mg/L and 2mg/L
About 4# 10mg/L and 5mg/L
In nutrient solution, replenish proper amount of carbon source simultaneously, simulation eutrophic pollution water quality.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out high-temperature sterilization about 30 minutes at 120 ℃, be cooled to room temperature, testing column adopts the sterilization of UV radiation method;
(3) will take from the heavily contaminated river bottom mud of original position, and be packed into the column bottom from the testing column bottom by original appearance, and make thickness reach 20cm, the adjustment proper height makes the water and soil interface flush with first pore water sample tap, and outer the survey wrapped up aluminium-foil paper and made the bed mud side be the shading state;
(4) choose the healthy and strong waterweed of certain biomass growing way in position, the about 10cm of every strain submerged plant length is planted in submerged plant on the bed mud uniformly;
(5) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level through pump;
(6) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and sediment with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(7) each factor in the restoration of the ecosystem simulation post is carried out observation analysis, regularly COD, the NH in the test simulator water body
4 +, NO
3 -Deng water quality parameter, analyzing water body nutritive salt removal effect and restoration of the ecosystem effect;
In advance incubation time is one month, and microbe carrier is changed with the nutrient solution replacement cycle and is a week, and viewing test post implants growing state treats that root system of plant grows up to, after microorganism fully is diffused in water body and the sediment, cultivation end in advance.More renew nutrient solution then, in the nutrient solution storage tank from the water inlet every day water sampling, detect wherein COD, NH
4 +, NO
3 -Or the like water-quality guideline, experiment finishes behind the 8d.
Behind the 8d, 1#, 2#, 3#, 4# ammonia nitrogen removal frank are respectively: 87.6%, 83.3%, 89.9%, 86.7%.Experimental result is shown in figure 14.
1#, 2#, 3#, 4# nitre nitrogen removal efficiency are respectively: 74%, 81.4%, 88.5%, 77.9%.Experimental result is shown in figure 15.
Claims (8)
1. a multi-functional lake ecological is repaired analogue means; It is characterized in that a kind of cylindrical transparent unit; Every separated certain distance is provided with sample tap; Repair the requirement of means gathers the original position bed mud, adds denitrification microorganism, hydrophyte, a series of environmental impact factors of regulation and control water body: water nutrition salinity, temperature, light intensity according to different lake ecologicals; The lake or the river bottom mud sediment of this analogue means bottom filling in-situ acquisition; Add the restoration of the ecosystem of different aquatic plant simulation lacustrine plant; Add the restoration of the ecosystem of different proportioning hydrophyte simulation lacustrine plant; Utilize the microbe carrier of pore mesh bag parcel in cylinder, to add the reparation of denitrification microorganism simulation microbial ecological; Add plant and denitrification microorganism simulating plant-microorganism coupling restoration of the ecosystem; Or through regulating and control water temperature by adding hot water storgae, built-in temperature probe and outreaching the temperature control system that temperature controller forms; Through being furnished with the halogen light source regulating system intensity of illumination of light intensity control, simulate different water body in lake environmental baselines; At this device differing heights the sample tap sampling is set, through measuring water body and pore water nutritive salt, pH, ORP, DO, N
2, bed mud denitrifying bacterium quantity physical and chemical index, research water nutrition salt is removed ability and denitrogenation speed, thus effectively simulation and the different lake ecologicals of comparative study are repaired means.
2. multi-functional lake ecological according to claim 1 is repaired analogue means, it is characterized in that Halogen lamp LED intensity of illumination range of control 0～10000Lux, 10～45 ± 1 ℃ of culture-liquid temp.
3. multi-functional lake ecological according to claim 1 is repaired analogue means, it is characterized in that being positioned at the outer nutrient solution storage tank of testing column and is connected with testing column top with the nutrient solution import through supercharge pump.
4. multi-functional lake ecological according to claim 1 is repaired the method for application of analogue means, it is characterized in that being made up of following steps:
(1) nutrient solution and testing column sterilization: the nutrient solution that configures is put into high-pressure sterilizing pot, carried out high-temperature sterilization 30 minutes at 121 ℃, testing column adopts the sterilization of UV radiation method;
(2) adopt columnar sampler to gather original position bed mud sediment, lake or river bottom mud are packed into the column bottom from the testing column bottom by original appearance, the outer parcel aluminium-foil paper of surveying makes the shading of bed mud side;
(3) select submerged plant not of the same race, every strain submerged plant length 10cm is planted in submerged plant on the bed mud uniformly;
(4) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance through pump;
(5) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and sediment with the form of pore mesh bag parcel;
(6) under restoration of the ecosystem means of setting and environmental baseline, each factor in lake or the river channel ecology reparation simulation post is carried out observation analysis, regular sampling analysis.
5. multi-functional lake ecological according to claim 4 is repaired the method for application of analogue means; The adjustability that it is characterized in that nutrient solution chemical factor concentration: ammonia nitrogen concentration 0.1～10mg/L, nitre nitrogen concentration 0.1～5mg/L, N/P ratio 1～50, COD5～40mg/L, or freely select artificial distribution or natural water as nutrient solution.
6. multi-functional lake ecological according to claim 4 is repaired the method for application of analogue means, it is characterized in that the typical submerged plant that step (3) restoration of the ecosystem is selected for use is waterweed Elodea nuttallii, black algae Hydrilla verticilla, eel grass Vallisneria natans, little tooth bog pondweed P.maackianus.
7. multi-functional lake ecological according to claim 4 is repaired the method for application of analogue means, it is characterized in that the nitrogen cycle bacterium is ammonia oxidizing bacteria, nitrobacteria, nitrite bacteria, denitrifying bacteria.
8. multi-functional lake ecological according to claim 4 is repaired the method for application of analogue means, the application in the restoration of the ecosystem of lake or river drift or submerged plant or nitrogen cycle bacterium in position.
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| CN114946560B (en) * | 2022-06-13 | 2023-05-23 | 云南大学 | Method for evaluating adaptability of submerged plant to water exchange uniformity and application |
| CN116338131B (en) * | 2023-05-23 | 2023-08-22 | 中交第一航务工程局有限公司 | Test device and method for simulating microbial water quality restoration under different hydrodynamic conditions |
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| CN201110849Y (en) * | 2007-10-19 | 2008-09-03 | 中国科学院南京地理与湖泊研究所 | Indoor simulating device used for lake sediment-water interface process research |
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