CN102101725A - Multifunctional lake ecology restoration simulation device and use method as well as application - Google Patents
Multifunctional lake ecology restoration simulation device and use method as well as application Download PDFInfo
- Publication number
- CN102101725A CN102101725A CN2011100045051A CN201110004505A CN102101725A CN 102101725 A CN102101725 A CN 102101725A CN 2011100045051 A CN2011100045051 A CN 2011100045051A CN 201110004505 A CN201110004505 A CN 201110004505A CN 102101725 A CN102101725 A CN 102101725A
- Authority
- CN
- China
- Prior art keywords
- lake
- water
- nutrient solution
- simulation
- ecological
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention belongs to the technical field of ecological engineering, particularly relating to a multifunctional lake ecology restoration simulation device and use method as well as application. The device comprises a multifunctional ecology restoration simulation test cylinder, a light source control system and a water temperature control system, wherein the light source control system and the water temperature control system are positioned outside the top of the test cylinder; the outer surface of a reaction cylinder is provided with a top cover, a culture liquid inlet and a circulating water outlet; the middle of the reaction cylinder is provided with a water sampling port, and the lower part of the reaction cylinder is provided with the circulating water inlet, a substrate sludge pore water sampling port and a bottom plug; and the test cylinder internally comprises a nitrogen cycle bacteria carrier, submerged plants and settings. By using the method and device, the comprehensive regulation and control as well as optimization of parameters such as settings, submerged plants, denitrification microorganism, water temperature, light and water quality can be carried out, lake and inflowing riverway nutrient salt can be simulated and controlled, and a lake ecology restoration mechanism can be studied. The device disclosed by the invention is simple in structure, is strong in mobility, is diversified in restoration simulation function, is simple in manufacturing, is low in cost and is easy to operate, and can be used indoor and outdoor.
Description
Technical field
The invention belongs to the ecological engineering technology field, be specifically related to method and apparatus and using method and application that a kind of multi-functional simulation lake ecological is repaired.
Background technology
Quickening along with China's rapid economy development and town and country construction, along the river the discharging of pollutents such as area, lake nitrogen, phosphorus, organism increases year by year, the pollution becoming serious of lake and stream entering lake water surrounding, show as mainly that water area falls sharply, obliteration of lake is serious, lake and river channel ecology system damage, lake eutrophication aggravation, its reason mainly contains: regional industry sewage along the lake, sanitary sewage, agricultural area source discharging are to the pollution of water body in lake; The lake seining cultivation has destroyed lake aquatic attitude system, and the bait of input quickens the eutrophication in lake; Ring lake basin topsoil dried wet deposition causes the lake Effects of water environment.
Body eutrophication is meant that water body such as lake admits nutritional substances such as excessive nitrogen, phosphorus, algae and other hydrobionts are bred unusually, water transparency and dissolved oxygen change, cause the lake water quality deterioration, the acceleration lake is aging, thereby makes lake ecosystem and water function be subjected to hindering and the destructive phenomenon.Lake eutrophication has become one of limiting factor of restriction social economy Sustainable development.
At present, the focus of the research of the eutrophication control of water body in lake mainly concentrates on the control techniques to nutritive salt, and for example aquatic higher plant nutritive salt absorbs reparation, immobilized microorganism technique etc.Aquatic higher plant recovery technique adopts waterplant to repair more, comprise (number of patent applications 03139848.0 such as emergent, pleuston, submerged plant, a kind of plant restoration method of eutrophication water), immobilized microorganism technique utilizes the removal of microorganisms water pollutant (number of patent application 200510038832.3, efficient nitrogen-cycle bacteria artificial screening and the application in Ecological Recovery of Water and water treatment) of enrichment on the carrier etc.
Main pleuston, emergent, and the local endemic species such as submerged plant of adopting in aquatic higher plant recovery technique, by the nutritive substance in the water bodys such as a large amount of absorbed nitrogens of the growth metabolism of plant self, phosphorus, absorption suspended particulate, inhibition algal grown, enriching heavy metal (number of patent application 200410066162.1, a kind of method of treatig waste water containing chromium with aquatic plant) etc.Adopt the ecological bed technology of submergence type (number of patent application 200910034673.8, the ecological outstanding bed of a kind of submergence type of purifying eutrophication water body) can carry out depth of water adjustment according to transparency and water quality parameter, utilize submerged plant-water microorganisms system combined action in eutrophication water, to remove water nutrition salt.
Now, the lake scientist thinks that the limiting factor that causes body eutrophication mainly is a nitrogen phosphorus, and one of major objective of lake ecological reparation is exactly to control lake nutritive salt level, keeps lake clear water stable state.Though many scholars have carried out big quantity research to various lake ecological reparations, but present research mainly lays particular emphasis on lake treatment effect aspect, less relatively for the research of restoration of the ecosystem mechanism, how the optimum combination different ecological is repaired means just can make lake denitrogenation dephosphorizing maximized, still indeterminate at present.On the other hand, the method and apparatus that can simulate the restoration of the ecosystem of different lake environment condition, the different lake ecologicals reparation of contrast means treatment effect, discussion restoration of the ecosystem mechanism still remains to be developed, and has hindered the progress of lake ecological repair mechanisms.
It is actually rare to repair the mimic method and apparatus for lake ecological at present.There is the scholar to propose a kind of Wetland micro-ecological simulation method, be intended to the wetland bed mud sample of field acquisition is placed on formation wetland post in the PVC cylindrical vessel respectively, realize the simulation Wetlands ecosystems are carried out temperature range control and detected (number of patent application 200910098001.3, a kind of Wetland micro-ecological simulation method) by temperature control device then; Also there is the scholar to research and develop equipment simulating lake sediment-water and soil interface environments, artificially controls interfacial water energetic disturbance, interface illumination, interface temperature and overlying water chemical property, research water and soil interface biological process mechanism.(number of patent application 200710133406.7, a kind of indoor simulated system that is applied to lake sediment-water interface process research).
Though Chinese scholars has been carried out certain research to water body in lake eutrophication restoration of the ecosystem simulation, and a series of technology have been researched and developed, but up to the present, factors such as original position lake sediment, overlying water, submerged plant, nitrogen cycle bacterium can be incorporated into the multifunctional ecological reparation simulation technique method of studying in the system and yet there are no report.Adopt manual simulation's means of different lake ecological repair process, comprise comprehensive regulation and optimizations such as settling, submerged plant, denitrification microorganism, water temperature, illumination and water quality parameter, the multi-functional simulator of control lake nutritive salt, research lake ecological repair mechanisms does not have exploitation as yet.Therefore, improvement has positive effect to nutrition-enriched water of lake to study this type of technology and device.
Summary of the invention
The technical issues that need to address of the present invention are to be difficult to simulate different lake ecologicals reparation means at serious further lake eutrophication problem and existing apparatus, the invention provides a kind of method and apparatus that different lake ecologicals are repaired means of simulating, can effectively simulate different lake ecologicals and repair means, thereby can promote the research of original position lake ecological repair mechanisms.Use can the manual simulation different lake ecological repairing environments of this device, regulation and control physics, chemistry, biotic factor, study of the influence of each factor, promote various combination restoration of the ecosystem means control lake nutritive salt, improve the research of water body in lake ecological environmental condition water body in lake restoration of the ecosystem, denitrogenation dephosphorizing.
It is a kind of cylindrical transparent unit that multi-functional lake ecological of the present invention is repaired simulator, every certain distance thief hole is set, repair the requirement of means gathers the original position bed mud, adds denitrification microorganism, waterplant, a series of environmental influence factors of regulation and control water body: water nutrition salt concn, temperature, light intensity according to different lake ecologicals; The lake or the river bottom mud settling of this simulator bottom filling in-situ acquisition; Add the restoration of the ecosystem of different waterplant simulation lacustrine plant; Add the restoration of the ecosystem of different proportioning waterplant simulation lacustrine plant; Utilize the microbe carrier of pore mesh bag parcel in cylinder, to add the reparation of denitrification microorganism simulation microbial ecological; Add plant and denitrification microorganism simulating plant-microorganism coupling restoration of the ecosystem; Also can be by by adding hot water tank, built-in temperature temp probe and outreaching the temperature controlling system regulation and control water temperature that temperature regulator forms; By being furnished with the halogen light source regulation system intensity of illumination of light intensity control, simulate different water body in lake envrionment conditionss; At this device different heights the thief hole sampling is set, by measuring water body and pore water nutritive salt, pH, ORP, DO, N
2, bed mud denitrifying bacteria quantity physical and chemical index, research water nutrition salt is removed ability and denitrogenation speed, thus effectively simulation and the different lake ecologicals of comparative study are repaired means.The device accompanying drawing is seen Figure 16.
Technical solution of the present invention:
A kind of device of simulating the lake ecological reparation, light source outside comprising testing column and being positioned at the testing column top, the hot water tank that adds that water pipe is connected is arranged with testing column, the testing column top is a transparency cover, the testing column cartridge outer surface down sets gradually nutrient solution import, circulating water outlet from top, the centre is provided with the water quality thief hole, the bottom is provided with the import of recirculated water, settling pore water thief hole, and the bottom is provided with movable bottom plug.Alternative successively from top to bottom immobilization nitrogen cycle bacterium, submerged plant, planktonic organism, the bed mud placed in testing column inside.
Described top cover and bottom sealing plug are all detachable.Add temp probe is housed in the hot water tank, temp probe is associated with temperature regulator outward.Light source is equipped with light intensity control, can regulate the control light intensity.The outer nutrient solution storage tank of reaction column connects with reaction column by the nutrient solution import.
Intensity of illumination control: can select natural lighting or artificial lighting dual mode, when the testing column device adopts halogen light source 8 irradiations,, adopt light intensity control 7 to regulate light source control intensity of illumination, light intensity regulating scope 0Lux ~ 10000Lux with light intensity instrumentation amount; The original position intensity of illumination is logical can cross automatic weather station monitoring acquisition experimental data.
Temperature control:, testing column adopts constant temperature jacket to carry out controlled temperature by temperature regulator 3, and the control culture-liquid temp is at 10～45 ± 1 ℃, and in-situ temperature can obtain data by the automatic weather station monitoring.
Nutritive salt control: according to original position aquatic monitoring water quality parameter, the nutrient solution of configuration simulation lake, river water quality, selectable through pressure kettle 121 ℃ of high-temperature sterilization 30min, by the nutrient solution storage tank by topping-up pump according to certain flow, through nutrient solution inlet injection test post.
Immobilized microorganism is thrown in: discharge four kinds of indigenous nitrogen cycle bacterium (Ammonifying bacteria, nitrite bacteria, nitrobacteria, and denitrifying bacterium) by fixed microorganism carrier, with pore mesh bag coated carrier, carrier weight in wet base 50g/ bag, be fixed in top cover for one section by fine rule, carrier is hung on testing column top water body, and the microbe carrier replacing time is a week.
A kind of using method of multi-functional simulation lake ecological prosthetic device:
(1) nutrient solution and testing column sterilization: the nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of uv-radiation method;
(2) adopt columnar sampler to gather original position bed mud settling, the bottom mud in lake that to take from original position is packed into the column bottom from the testing column bottom, and thickness reaches 20cm, is adjusted to proper height, the water and soil interface is flushed with first pore water thief hole, and the outer parcel aluminium-foil paper of surveying makes the bed mud side be the shading state;
(3) select the healthy and strong submerged plant of certain biomass growing way, the about 10cm of every strain submerged plant length evenly is planted on the bed mud;
(4) with sterilization back nutrient solution in 1, inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level by pump;
(5) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and settling with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(6) under restoration of the ecosystem means of setting and envrionment conditions, each factor in the lake ecological reparation simulation post is carried out observation analysis, regularly pH, ORP, DO, TP, TN, the NH in the test simulator water body
4 +, NO
3 -, NO
2 -, solvability N
2Deng, analyzing water body nutritive salt removal effect and restoration of the ecosystem effect;
In the step (3) submerged plant can according to different lakes submerged vegetation feature selection typical case submerged plant waterweed (
Elodea nuttallii), black algae (
Hydrilla verticilla), eel grass (
Vallisneria natans), little tooth Herba potamogetonis distincti (
P.maackianus).
The invention has the beneficial effects as follows provides a kind of method and apparatus that the lake different ecological is repaired means of simulating, and combines reparation means such as bed mud settling denitrification, submerged plant absorption, the effect of denitrogenation circulation bacterium.Apparatus of the present invention can be simulated different lake ecologicals and repair means, as means such as plant, microorganism, plant-microorganism couplings, control multiple physics, chemistry, biotic factor, as temperature, intensity of illumination, concentration of nitrogen and phosphorus, submerged plant, nitrogen cycle bacteria biomass etc., the experiment contrast effect is obvious, of the influence of various environmental factorss be can effectively simulate, thereby theoretical foundation and technical support provided for the lake ecological reparation the lake ecological repair mechanisms.
Different ecological reparation means that apparatus of the present invention are integrated, both can investigate decontamination effect improving and the mechanism of single method to water body in lake, also can contrast between the different methods, perhaps the processing efficiency during the above combined action of two kinds of methods is studied each factor removes ability to lake ecological reparation, nitrogen phosphorus the size that influences.
Apparatus of the present invention are simple in structure, and cost is low, and are easy to operate, easy to make, and diverse in function can be carried out the simulation comparative study that different ecological is repaired means easily.Therefore, this invention is the efficient apparatus of simulation eutrophic lake restoration of the ecosystem means, the ecological repair mechanisms of research.
Description of drawings
Fig. 1, Fig. 2, Fig. 3, Fig. 4. embodiment 1 lab scale test nitrogen nutrition salt is changing trend diagram in time,-■-ammonia nitrogen concentration (mg/L),-●-nitric nitrogen concentration (mg/L) ,-▲-nitrite nitrogen concentration (mg/L) ,-▼-total nitrogen concentration (mg/L);
Fig. 5. embodiment 2 lab scales test denitrifying bacteria quantity is variation tendency in time ,-■-settling post (* 10
5Cells/g) ,-●-settling+waterweed post (* 10
5Cells/g) ,-▲-settling+waterweed+microorganism (* 10
5Cells/g);
Fig. 6. embodiment 3 field trial condition of different temperatures influence rule, 1#:10 ℃, 2#:18 ℃, 3#:25 ℃, 4#:35 ℃ to plant-microorganism coupling restoration of the ecosystem denitrogenation speed;
Fig. 7. embodiment 4 field trial different ecologicals are repaired water body dissolved oxygen variation tendency under the means condition ,-■-settling post (umol/L) ,-●-settling+black algae post (umol/L) ,-▲-settling+black algae+nitrogen cycle bacterial spawn (umol/L);
Fig. 8. embodiment 4 field trial different ecologicals are repaired water body pH variation tendency under the means condition ,-■-settling post ,-●-settling+black algae post ,-▲-settling+black algae+nitrogen cycle bacterial spawn;
Fig. 9. four kinds of restoration of the ecosystem submerged plants of embodiment 5 shop experiments TN clearance ,-■-eel grass (mg/L) ,-●-waterweed (mg/L) ,-▲-little tooth Herba potamogetonis distincti (mg/L) ,-▼-black algae (mg/L);
Simulation test plant-microorganism coupling nutritive salt variation tendency is in time repaired in Figure 10: embodiment 6 river courses ,-■-total nitrogen concentration (mg/L) ,-●-total phosphorus concentration (mg/L) ,-▲-ammonia nitrogen concentration (mg/L) ,-▼-COD(mg/L)
Waterweed post water body dissolved oxygen concentration under Figure 11: the embodiment 7 shop experiment different illumination conditions ,-■-2000Lux(umol/L) ,-●-3000Lux(umol/L) ,-▲-4000Lux(umol/L) ,-▼-6000Lux(umol/L);
Figure 12. the different lake region settling nitrogen cycle bacterium propagation of embodiment 8 field trials trend ,-■-Mei Liang gulf dredging area (* 10
5Cells/g) ,-●-tribute arm of lake (* 10
5Cells/g) ,-▲-river course (* 10
5Cells/g) ,-▼-eastern Taihu Lake (* 10
5Cells/g);
Figure 13. embodiment 9 shop experiment submerged plants are repaired mesoporosity water TN and change ,-■-black algae post (mg/g dry weight) ,-●-to (mg/g dry weight) in the same old way;
Figure 14. embodiment 10 field trial plant-microorganism couplings are to different quality ammonia nitrogen removal effect ,-■-1# (mg/L) ,-●-2#(mg/L) ,-▲-3#(mg/L) ,-▼-4#(mg/L);
Figure 15. embodiment 10 field trial plant-microorganism couplings are to different quality nitre nitrogen removal effect ,-■-1# (mg/L) ,-●-2#(mg/L) ,-▲-3#(mg/L) ,-▼-4#(mg/L);
Figure 16. multifunctional ecological is repaired the simulator structural representation, adds hot water tank 1, temp probe 2, temperature regulator 3, topping-up pump 4, circulating water intake 5, circulating water outlet 6, light intensity control 7, halogen light source 8, nutrient solution import 9, denitrification microorganism carrier 10, testing column 11, water body thief hole 12, submerged plant (waterweed) 13, bed mud pore water thief hole 14, settling 15, top cover 16, seal film 17, nutrient solution storage tank 18, bottom plug 19.
Embodiment
The present invention will be further described below in conjunction with drawings and Examples:
Indoor lab scale (investigate settling, plant, microorganism in lake ecological is repaired to the nutritive salt removal effect)
The device of simulated ecological reparation comprises topping-up pump 4, nutrient solution import 9, and denitrification microorganism carrier 10, testing column 11, water body thief hole 12, submerged plant (waterweed) 13, settling 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Because this lab scale uses natural lighting and only need satisfy the condition of different tests post as room temperature, add hot water tank 1, temp probe 2, temperature regulator 3, circulating water intake 5, circulating water outlet 6, light intensity control 7, halogen light source 8 and be not provided with at this embodiment.Testing column 11 cartridge outer surface thief holes 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Denitrification microorganism carrier weight in wet base 50g is fixed on testing column inside from liquid level 10cm place with the pore mesh bag.
The nutrient solution preparation adds with ammonium sulfate and SODIUMNITRATE, makes that ammonia nitrogen concentration and the nitric nitrogen concentration in the nutrient solution reaches respectively about 2.0mg/L and 1.0mg/L, and replenishes proper amount of carbon source in nutrient solution, simulation eutrophic pollution water quality.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of uv-radiation method;
(3) will take from the bottom mud in lake of original position, be packed into the column bottom by original appearance, be adjusted to proper height, the water and soil interface is flushed with first pore water thief hole from the testing column bottom; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(4) choose the healthy and strong waterweed of certain biomass growing way, the about 10cm of every strain submerged plant length evenly is planted on the bed mud;
(5) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level by pump;
(6) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and settling with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(7) each factor in the lake ecological reparation simulation post is carried out observation analysis, regularly pH, ORP, DO, the NH in the test simulator water body
4 +, NO
3 -, NO
2 -Deng water quality parameter, analyzing water body nutritive salt removal effect and restoration of the ecosystem effect;
1# testing column (only adding settling) step: (2), (3), (5), (7)
2# testing column (settling+waterweed) step: (2), (3), (4), (5), (7)
3# testing column (settling+microorganism) step: (1), (2), (3), (5), (6), (7)
4# testing column (settling+microorganism+waterweed) step: (1), (2), (3), (4), (5), (6), (7)
Pre-incubation time is one month, and microbe carrier is changed and the nutrient solution replacement cycle is a week, and viewing test post implants growing state treats that root system of plant grows up to, after microorganism fully is diffused in water body and the settling, pre-cultivation end.More renew nutrient solution then, in the nutrient solution storage tank from the water inlet every day water sampling, detect wherein TN, NH
4 +-N, NO
2 --N, NO
3 -Water-quality guideline such as-N, experimental result such as Fig. 1, Fig. 2, Fig. 3, shown in Figure 4.Behind the 8d, four kinds of different ecological reparation means (corresponding 1#, 2#, 3#, 4# restoration of the ecosystem testing column) are respectively the clearance of ammonia nitrogen, nitric nitrogen, total nitrogen:
1# testing column (only adding settling): 27.4%, 41.8%, 32.2%.
2# testing column (settling+waterweed): 89.5%, 79.6%, 87.1%.
3# testing column (settling+nitrogen cycle bacterium): 72.3%, 79.1 % 83.3%.
4# testing column (settling+microorganism+waterweed): 93.2%, 81.4%, 89.1%.
By experimental result as can be seen, when adopting submerged plant and the coupling of nitrogen cycle bacterium to repair means, water nutrition salt is removed most effective.Contrast only exists the testing column of lake sediment not good to the removal efficient of water nutrition salt.
Four kinds of nitrogen cycle bacterium of table 1. multiplication culture based formulas
| Chemical ingredients (analytical pure) | Four kinds of bacterium combinations |
| C 6H 12O 6 | 1g |
| (NH4) 2SO 4 | 0.44g |
| KNO 3 | 0.5g |
| NaNO 2 | 0.2g |
| KH 2PO 4 | 0.25g |
| MgSO 4 | 0.02g |
| NaCl | 0.02g |
| FeCl 3 | 0.02g |
| CoCl 2 | 0.002g |
| H 2O | 1L |
Embodiment 2
Indoor lab scale (investigate different lake ecologicals and repair the influence of means) to settling denitrifying bacteria quantity
The device of simulated ecological reparation comprises and adds hot water tank 1, temp probe 2, temperature regulator 3, topping-up pump 4, circulating water intake 5, circulating water outlet 6, light intensity control 7, halogen light source 8, nutrient solution import 9, denitrification microorganism carrier 10, testing column 11, water body thief hole 12, submerged plant (waterweed) 13, settling 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface thief holes 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Denitrification microorganism carrier weight in wet base 50g is fixed on testing column inside from liquid level 10cm place with the pore mesh bag.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of uv-radiation method;
(3) will take from the bottom mud in lake of original position, be packed into the column bottom by original appearance, be adjusted to proper height, the water and soil interface is flushed with first pore water thief hole from the testing column bottom; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(4) choose the healthy and strong waterweed of certain biomass growing way, the about 10cm of every strain submerged plant length is planted on the bed mud uniformly;
(5) with sterilization back nutrient solution in 1, inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level by pump;
(6) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and settling with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(7) restoration of the ecosystem simulation test column device is put under the indoor environmental condition that sets, light source adopts halogen lamp 8, photoperiod 12h/12h; Intensity of illumination 4000Lux(is by light intensity control 7 controls); 20 ± 1 ℃ of temperature (the chuck water bath with thermostatic control is by temperature regulator 3 controls).Testing column is carried out pre-cultivation of three weeks.
(8) after pre-cultivation finishes, get settling once in a week, adopt the MPN method to measure the quantity of denitrifying bacteria in the settling, MPN method denitrifying bacteria substratum adopts the Giltay substratum, sees Table 2.
1# testing column (only adding settling) step: (2), (3), (5), (7), (8)
2# testing column (settling+waterweed) step: (2), (3), (4), (5), (7), (8)
3# testing column (settling+microorganism+waterweed) step: (1), (2), (3), (4), (5), (6), (7), (8)
This off-test of back around the sampling, experimental result is seen Fig. 5, along with the release of nitrogen cycle bacterium in carrying out of cultivating and the carrier, and compares in the bed mud that does not add the nitrogen cycle bacterium, denitrifying bacteria quantity increases obviously in the bed mud after the interpolation.
Embodiment 3
Field trial (investigate temperature factor lake ecological is repaired the influence of denitrogenation speed)
The device of simulated ecological reparation comprises and adds hot water tank 1, temp probe 2, temperature regulator 3, topping-up pump 4, circulating water intake 5, circulating water outlet 6, nutrient solution import 9, denitrification microorganism carrier 10, testing column 11, water body thief hole 12, submerged plant (eel grass) 13, settling 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface thief holes 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Natural lighting is adopted in the Yin Ben experiment, so light intensity control 7, halogen light source 8 is not provided with.
The experimental technique step:
(1) nutrient solution and testing column sterilization:,, inject the nutrient solution storage tank as restoration of the ecosystem simulation test post nutrient solution at Taihu Lake Mei Liangwan in-situ acquisition capacity lake water;
(2) will take from the bottom mud in lake of original position, and be packed into the column bottom from the testing column bottom by original appearance, bed mud thickness is 20cm, is adjusted to proper height, and the water and soil interface is flushed with first pore water thief hole; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(3) choose the healthy and strong eel grass of certain biomass growing way, the about 15cm of every strain submerged plant length is planted on the bed mud uniformly;
(4) with nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level by pump;
(5) restoration of the ecosystem simulation test column device is put under the outdoor environment natural lighting condition that sets, outdoor natural light is about 3900Lux-4100Lux, about 10h/14h of photoperiod according to intensity; Temperature is divided into 15 ℃, 35 ℃ of two gradients (the chuck water bath with thermostatic control is by temperature regulator 3 controls), and the external temperature average is 25 ℃.Testing column is carried out pre-cultivation of three weeks.
(6) each factor in the lake ecological reparation simulation post is carried out observation analysis, the pre-cultivation finished back DO, the NH in the test simulator water body regularly
4 +, NO
3 -, NO
2 -, the TN water quality parameter, analyzing water body denitrification rate and restoration of the ecosystem effect;
1#, 2#, 3#, 4# testing column temperature are set to 10 ℃, 18 ℃, 25 ℃, 35 ℃ respectively.
Water sampling detected DO, NH in the water body in continuous five days
4 +, NO
3 -, NO
2 -, water quality parameter such as TN, utilize the nitrogen null readings to calculate water and soil interface denitrification rate in the testing column, experimental result such as Fig. 6, at identical illumination condition, under the condition of different temperatures, in the time of 25 ℃, system's denitrification rate is the highest as can be seen.
Field trial (investigating the variation tendency of water quality parameter pH, ORP etc. under the different lake ecological repairing conditions)
The device of simulated ecological reparation comprises topping-up pump 4, circulating water intake 5, circulating water outlet 6, nutrient solution import 9, denitrification microorganism carrier 10, testing column 11, water body thief hole 12, submerged plant (black algae) 13, settling 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface thief holes 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Natural lighting is adopted in the Yin Ben experiment, and not to temperature adjusting, so add hot water tank 1, temp probe 2, temperature regulator 3, light intensity control 7, halogen light source 8 is not provided with.Denitrification microorganism carrier weight in wet base 50g is fixed on testing column inside from liquid level 10cm place with the pore mesh bag.
The nutrient solution preparation adds with ammonium sulfate and SODIUMNITRATE, makes that ammonia nitrogen concentration and the nitric nitrogen concentration in the nutrient solution reaches respectively about 2.0mg/L and 1.0mg/L, and replenishes proper amount of carbon source in nutrient solution, simulation eutrophic pollution water quality.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of uv-radiation method;
(3) will take from the bottom mud in lake of original position, and be packed into the column bottom from the testing column bottom by original appearance, thickness is 20cm, is adjusted to proper height, and the water and soil interface is flushed with first pore water thief hole; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(4) choose the healthy and strong black algae of certain biomass growing way in position, the about 10cm of every strain submerged plant length is planted on the bed mud uniformly;
(5) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level by pump;
(6) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and settling with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(7) each factor in the lake ecological reparation simulation post is carried out observation analysis, pH, the ORP in the regular test simulator water body, DO,
-Deng water quality parameter, analyze different ecological and repair the influence of means the water quality parameter;
1# testing column (only adding settling) step: (2), (3), (5), (7)
2# testing column (settling+waterweed) step: (2), (3), (4), (5), (7)
3# testing column (settling+microorganism+waterweed) step: (1), (2), (3), (4), (5), (6), (7)
Pre-incubation time is one month, and microbe carrier is changed and the nutrient solution replacement cycle is a week, and viewing test post implants growing state treats that root system of plant grows up to, after microorganism fully is diffused in water body and the settling, pre-cultivation end.More renew nutrient solution then, in the nutrient solution storage tank, in from the water inlet 10 hours, per hour get water sample one time, detect water quality parameters such as DO, pH wherein.Experimental result such as Fig. 7, shown in Figure 8.As can be seen, when utilizing submerged plant to carry out the lake ecological reparation, because light of submerged plant and effect discharge oxygen, DO concentration will be apparently higher than the water body that does not adopt the submerged plant reparation in the water.When using phytoremediation separately, water body pH raises very fast, and pH drops to about 9 after continuing to add microorganism.
Shop experiment (investigate in the different submerged plant lake ecologicals reparations nutritive salt is removed ability)
The device of simulated ecological reparation comprises, topping-up pump 4, circulating water intake 5, circulating water outlet 6, light intensity control 7, halogen light source 8, nutrient solution import 9, denitrification microorganism carrier 10, testing column 11, water body thief hole 12, submerged plant (eel grass) 13, settling 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface thief holes 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Incubated at room temperature is adopted in the Yin Ben experiment, thus add hot water tank 1, temp probe 2, temperature regulator 3 is not provided with.
The nutrient solution preparation adds with ammonium sulfate and SODIUMNITRATE, makes that ammonia nitrogen concentration and the nitric nitrogen concentration in the nutrient solution reaches respectively about 2.0mg/L and 1.0mg/L, and replenishes proper amount of carbon source in nutrient solution, simulation eutrophic pollution water quality.
The experimental technique step:
(1) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of uv-radiation method;
(2) will take from the bottom mud in lake of original position, and be packed into the column bottom from the testing column bottom by original appearance, bed mud thickness is 20cm, is adjusted to proper height, and the water and soil interface is flushed with first pore water thief hole, and outer the survey wrapped up aluminium-foil paper and made the bed mud side be the shading state;
(3) choose the healthy and strong eel grass of certain biomass growing way, waterweed, little tooth Herba potamogetonis distincti, black algae, the about 10cm of every strain submerged plant length is planted in different submerged plants on some cultivation post bed muds respectively uniformly;
(4) with nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level by pump;
(5) restoration of the ecosystem simulation test column device is put under the indoor conditions that sets light intensity 4000Lux, about 12h/12h of photoperiod; Temperature is a room temperature.Testing column is carried out month pre-a cultivation.
(6) each factor in the lake ecological reparation simulation post is carried out observation analysis, the pre-cultivation finished back DO, the NH in the test simulator water body regularly
4 +, NO
3 -, NO
2 -, the TN water quality parameter, analyzing water body denitrification rate and restoration of the ecosystem effect;
The pre-cultivation more renews nutrient solution after finishing, in the nutrient solution storage tank from the water inlet every day water sampling, detect wherein TN, NH
4 +-N, NO
2 --N, NO
3 -Water-quality guideline such as-N, experimental result are as shown in Figure 9.Behind the 8d, four kinds of submerged plants (corresponding 1#, 2#, 3#, 4# restoration of the ecosystem testing column) are respectively the clearance of total nitrogen:
1# testing column (eel grass): 52.5%
2# testing column (waterweed): 88.7%
3# testing column (little tooth Herba potamogetonis distincti): 66.3 %.
4# testing column (black algae): 82.9%.
Simulation test (investigating plant, the reparation of microorganism coupling river channel ecology to nutritive salt removal efficient) is repaired in the river course
The device of simulated ecological reparation comprises topping-up pump 4, nutrient solution import 9, and denitrification microorganism carrier 10, testing column 11, water body thief hole 12, submerged plant (waterweed) 13, settling 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Because this lab scale uses natural lighting and only need satisfy the condition of different tests post as room temperature, add hot water tank 1, temp probe 2, temperature regulator 3, circulating water intake 5, circulating water outlet 6, light intensity control 7, halogen light source 8 and be not provided with at this embodiment.Testing column 11 cartridge outer surface thief holes 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Denitrification microorganism carrier weight in wet base 50g is fixed on testing column inside from liquid level 10cm place with the pore mesh bag.
The nutrient solution preparation adds with ammonium sulfate and SODIUMNITRATE, makes that ammonia nitrogen concentration and the nitric nitrogen concentration in the nutrient solution reaches respectively about 4mg/L and 2mg/L, simulation heavily contaminated river course eutrophic pollution water quality.In nutrient solution, add simultaneously glucose to replenish proper amount of carbon source.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of uv-radiation method;
(3) will take from the heavily contaminated river bottom mud of original position, be packed into the column bottom by original appearance, and make thickness reach 20cm, adjust proper height, the water and soil interface is flushed with first pore water thief hole from the testing column bottom; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(4) choose the healthy and strong waterweed of certain biomass growing way in the river course, the about 10cm of every strain submerged plant length is planted on the bed mud uniformly;
(5) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level by pump;
(6) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and settling with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(7) each factor in the river channel ecology reparation simulation post is carried out observation analysis, regularly TN, TP, DO, the NH in the test simulator water body
4 +, NO
3 -, water quality parameter such as COD, analyzing water body nutritive salt removal effect and restoration of the ecosystem effect;
Pre-incubation time is one month, and microbe carrier is changed and the nutrient solution replacement cycle is a week, and viewing test post implants growing state treats that root system of plant grows up to, after microorganism fully is diffused in water body and the settling, pre-cultivation end.More renew nutrient solution then, in the nutrient solution storage tank from the water inlet every day water sampling, detect wherein TN, NH
4 +-N, NO
3 -Water-quality guideline such as N, TP, COD.Behind the 10d, plant, microorganism coupling restoration of the ecosystem means are respectively the clearance of the total nitrogen of simulation river course waste water, total phosphorus, ammonia nitrogen, COD: 71.8%, 82.5%, 70.3%, 76.6%.Experimental result as shown in figure 10.
Embodiment 7
Shop experiment (investigating the variation of different light intensity condition cascading water plant oxygen release ability)
The device of simulated ecological reparation comprises, topping-up pump 4, circulating water intake 5, circulating water outlet 6, light intensity control 7, halogen light source 8, nutrient solution import 9, testing column 11, water body thief hole 12, submerged plant (eel grass) 13, settling 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface thief holes 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Incubated at room temperature is adopted in the Yin Ben experiment, and does not add microorganism, thus add hot water tank 1, temp probe 2, temperature regulator 3, denitrification microorganism carrier 10 is not provided with.
The experimental technique step:
(1) nutrient solution and testing column sterilization:,, inject the nutrient solution storage tank as restoration of the ecosystem simulation test post nutrient solution at Taihu Lake in-situ acquisition capacity lake water;
(2) will take from the bottom mud in lake of original position, and be packed into the column bottom from the testing column bottom by original appearance, bed mud thickness is 20cm, is adjusted to proper height, and the water and soil interface is flushed with first pore water thief hole; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(3) choose the healthy and strong waterweed of certain biomass growing way, the about 10cm of every strain submerged plant length is planted in different submerged plants on some cultivation post bed muds respectively uniformly;
(4) with nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level by pump;
(5) restoration of the ecosystem simulation test column device is put under the indoor conditions that sets, the light intensity gradient is 2000Lux, 3000Lux, 4000Lux, 6000Lux, about 12h/12h of photoperiod; Temperature is a room temperature.Testing column is carried out pre-cultivation of a week.
(6) each factor in the lake ecological reparation simulation post is carried out observation analysis, the pre-cultivation finished the back in 5 beginnings in morning, the DO in per two hours test simulator water bodys, 10 off-tests as a child;
Experimental result as shown in figure 11.In the range of light intensity of 2000 ~ 6000Lux, the oxygen release ability of waterweed in restoration of the ecosystem strengthens gradually as can be seen.
Embodiment 8
Field trial (investigate different lake regions and stream entering lake settling and throw in nitrogen cycle bacterium cultivation effect)
The device of simulated ecological reparation comprises topping-up pump 4, circulating water intake 5, and circulating water outlet 6, nutrient solution import 9, denitrification microorganism carrier 10, testing column 11, water body thief hole 12,, settling 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface thief holes 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Natural lighting is adopted in the Yin Ben experiment, and not to temperature adjusting, does not adopt submerged plant, thus add hot water tank 1, temp probe 2, temperature regulator 3, light intensity control 7, halogen light source 8, submerged plant 13 is not provided with.Denitrification microorganism carrier weight in wet base 50g is fixed on testing column inside from liquid level 10cm place with the pore mesh bag.
The nutrient solution preparation adds with ammonium sulfate and SODIUMNITRATE, makes that ammonia nitrogen concentration and the nitric nitrogen concentration in the nutrient solution reaches respectively about 2.0mg/L and 1.0mg/L, and replenishes proper amount of carbon source in nutrient solution, simulation eutrophic pollution water quality.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out being cooled to room temperature about high-temperature sterilization 30min at 121 ℃, testing column adopts the sterilization of uv-radiation method;
(3) will take from the Mei Liang gulf after tribute arm of lake, eastern Taihu Lake, the dredging and the bed mud in river course, remove root system of plant, be packed into the column bottom from the testing column bottom by original appearance, thickness is 20cm, be adjusted to proper height, the water and soil interface is flushed with first pore water thief hole; Outside parcel aluminium-foil paper makes the bed mud side be the shading state;
(5) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level by pump;
(6) fixation support with four kinds of nitrogen cycle bacterium of enrichment wraps up with the pore mesh bag, and the form that fine rule hangs is thrown in to water body liberating nitrogen circulation bacterium in water body and settling.The carrier replacement cycle is a week;
(7) each factor in the lake ecological reparation simulation post is observed, regularly the denitrifying bacteria number change in the test simulator bed mud;
The 1# testing column adds tribute arm of lake settling,
The 2# testing column adds eastern Taihu Lake Sediment
The 3# testing column adds dredging back plum beam bay sediment
The 4# testing column adds river drift
Incubation time is one month, and microbe carrier is changed and the nutrient solution replacement cycle is a week, and per week adopts MPN to measure.Experimental result as shown in figure 12.From experimental result as can be seen, behind the input nitrogen cycle bacterium, denitrifying bacteria quantity propagation is the fastest in the eastern Taihu Lake Sediment, and Mei Liang gulf, dredging back bed mud propagation is the slowest.
Embodiment 9
Shop experiment (investigating in the submerged plant restoration of the ecosystem influence) to pore water nutritive salt
The device of simulated ecological reparation comprises, topping-up pump 4, circulating water intake 5, circulating water outlet 6, light intensity control 7, halogen light source 8, nutrient solution import 9,, testing column 11, submerged plant (black algae) 13, pore water thief hole 14 settlings 15 seal film 17, nutrient solution storage tank 18 and bottom plug 19.Testing column 11 cartridge outer surface thief holes 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Incubated at room temperature is adopted in the Yin Ben experiment, thus add hot water tank 1, temp probe 2, temperature regulator 3, denitrification microorganism carrier 10 is not provided with.
The experimental technique step:
(1),, injects the nutrient solution storage tank as restoration of the ecosystem simulation test post nutrient solution at Taihu Lake in-situ acquisition capacity lake water;
(2) will take from the bottom mud in lake of original position, and be packed into the column bottom from the testing column bottom by original appearance, bed mud thickness is 20cm, is adjusted to proper height, and the water and soil interface is flushed with first pore water thief hole, and outer the survey wrapped up aluminium-foil paper and made the bed mud side be the shading state;
(3) choose the healthy and strong black algae of certain biomass growing way, the about 10cm of every strain submerged plant length is planted in different submerged plants on the bed mud uniformly;
(4) with nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level by pump;
(5) restoration of the ecosystem simulation test column device is put under the indoor conditions that sets light intensity 4000Lux, about 12h/12h of photoperiod; Temperature is a room temperature.Testing column is carried out month pre-a cultivation.
(6) each factor in the lake ecological reparation simulation post is carried out observation analysis, pre-TN, the NH that finishes in the test simulator settling of back that cultivate
4 +, NO
3 -, NO
2 -Deng water quality parameter;
The 1# testing column adds black algae, and the 2# testing column is sample in contrast, and experimental result as shown in figure 13.By experimental result as can be seen, behind the interpolation plant, the pore water TN in the shallow-layer bed mud has certain minimizing, and the following bed mud pore water of 5cm total nitrogen does not have considerable change.
Field trial (investigate plant, microorganism coupling and under the condition of water quality of different lakes, nutritive salt is removed efficient)
The device of simulated ecological reparation comprises topping-up pump 4, light intensity control 7, halogen light source 8, nutrient solution import 9, denitrification microorganism carrier 10, testing column 11, water body thief hole 12, submerged plant (waterweed) 13, settling 15 seals film 17, nutrient solution storage tank 18 and bottom plug 19.Because this lab scale uses natural lighting, adds hot water tank 1, temp probe 2, temperature regulator 3, circulating water intake 5, circulating water outlet 6, is not provided with at this embodiment.Testing column 11 cartridge outer surface thief holes 12 are respectively 25cm, 37cm, 45cm apart from the distance of bottom.Denitrification microorganism carrier weight in wet base 50g is fixed on testing column inside from liquid level 10cm place with the pore mesh bag.
The nutrient solution preparation adds with ammonium sulfate and SODIUMNITRATE, makes that ammonia nitrogen concentration and the nitric nitrogen concentration in the nutrient solution reaches respectively:
About 1# 1mg/L and 0.5mg/L,
About 2# 2mg/L and 1mg/L
About 3# 4mg/L and 2mg/L
About 4# 10mg/L and 5mg/L
In nutrient solution, replenish proper amount of carbon source simultaneously, simulation eutrophic pollution water quality.
The experimental technique step:
(1) carries out the enrichment culture of four kinds of nitrogen cycle bacterium with defined medium;
(2) nutrient solution and testing column sterilization: the 10L nutrient solution that configures is put into high-pressure sterilizing pot, carry out high-temperature sterilization about 30 minutes at 120 ℃, be cooled to room temperature, testing column adopts the sterilization of uv-radiation method;
(3) will take from the heavily contaminated river bottom mud of original position, and be packed into the column bottom from the testing column bottom by original appearance, and make thickness reach 20cm, and adjust proper height, the water and soil interface is flushed with first pore water thief hole, the outer survey wrapped up aluminium-foil paper and made the bed mud side be the shading state;
(4) choose the healthy and strong waterweed of certain biomass growing way in position, the about 10cm of every strain submerged plant length is planted in submerged plant on the bed mud uniformly;
(5) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance, arrive the height that requires until liquid level by pump;
(6) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and settling with the form of pore mesh bag parcel.The carrier replacement cycle is a week;
(7) each factor in the restoration of the ecosystem simulation post is carried out observation analysis, regularly COD, the NH in the test simulator water body
4 +, NO
3 -Deng water quality parameter, analyzing water body nutritive salt removal effect and restoration of the ecosystem effect;
Pre-incubation time is one month, and microbe carrier is changed and the nutrient solution replacement cycle is a week, and viewing test post implants growing state treats that root system of plant grows up to, after microorganism fully is diffused in water body and the settling, pre-cultivation end.More renew nutrient solution then, in the nutrient solution storage tank from the water inlet every day water sampling, detect wherein COD, NH
4 +, NO
3 -Or the like water-quality guideline, experiment finishes behind the 8d.
Behind the 8d, 1#, 2#, 3#, 4# ammonia nitrogen removal frank are respectively: 87.6%, 83.3%, 89.9%, 86.7%.Experimental result as shown in figure 14.
1#, 2#, 3#, 4# nitre nitrogen removal efficiency are respectively: 74%, 81.4%, 88.5%, 77.9%.Experimental result as shown in figure 15.
Claims (8)
1. a multi-functional lake ecological is repaired simulator, it is characterized in that a kind of cylindrical transparent unit, every certain distance thief hole is set, repair the requirement of means gathers the original position bed mud, adds denitrification microorganism, waterplant, a series of environmental influence factors of regulation and control water body: water nutrition salt concn, temperature, light intensity according to different lake ecologicals; The lake or the river bottom mud settling of this simulator bottom filling in-situ acquisition; Add the restoration of the ecosystem of different waterplant simulation lacustrine plant; Add the restoration of the ecosystem of different proportioning waterplant simulation lacustrine plant; Utilize the microbe carrier of pore mesh bag parcel in cylinder, to add the reparation of denitrification microorganism simulation microbial ecological; Add plant and denitrification microorganism simulating plant-microorganism coupling restoration of the ecosystem; Also can be by regulating and control water temperature by adding hot water tank, built-in temperature probe and outreaching the temperature controlling system that temperature regulator forms; By being furnished with the halogen light source regulation system intensity of illumination of light intensity control, simulate different water body in lake envrionment conditionss; At this device different heights the thief hole sampling is set, by measuring water body and pore water nutritive salt, pH, ORP, DO, N
2, bed mud denitrifying bacteria quantity physical and chemical index, research water nutrition salt is removed ability and denitrogenation speed, thus effectively simulation and the different lake ecologicals of comparative study are repaired means.
2. multi-functional lake ecological according to claim 1 is repaired simulator, it is characterized in that halogen lamp intensity of illumination span of control 0～10000Lux, 10～45 ± 1 ℃ of culture-liquid temp.
3. multi-functional lake ecological according to claim 1 is repaired simulator, it is characterized in that being positioned at the outer nutrient solution storage tank of testing column and is connected with The columns top with the nutrient solution import by topping-up pump.
4. multi-functional lake ecological according to claim 1 is repaired the using method of simulator, it is characterized in that being made of following steps:
(1) nutrient solution and testing column sterilization: the nutrient solution that configures is put into high-pressure sterilizing pot, carry out high-temperature sterilization about 30 minutes at 121 ℃, testing column adopts the sterilization of uv-radiation method;
(2) adopt columnar sampler to gather original position bed mud settling, lake or river bottom mud are packed into the column bottom from the testing column bottom by original appearance, the outer parcel aluminium-foil paper of surveying makes the shading of bed mud side;
(3) select submerged plant not of the same race, the about 10cm of every strain submerged plant length is planted in submerged plant on the bed mud uniformly;
(4) with sterilization back nutrient solution in (1), inject in the sterilized simulation test post with suitable flow, as far as possible bed mud is not produced big disturbance by pump;
(5) fixation support of four kinds of nitrogen cycle bacterium of enrichment is thrown in to water body liberating nitrogen circulation bacterium in water body and settling with the form of pore mesh bag parcel;
(6) under restoration of the ecosystem means of setting and envrionment conditions, each factor in lake or the river channel ecology reparation simulation post is carried out observation analysis, regular sampling analysis.
5. simulation according to claim 4 lake different ecological is repaired the method for means, the adjustability that it is characterized in that nutrient solution chemical factor concentration: ammonia nitrogen concentration 0.1～10mg/L, nitre nitrogen concentration 0.1～5mg/L, N/P ratio 1～50, COD5～40mg/L, also can freely select artificial distribution or natural water as nutrient solution.
6. simulation according to claim 4 lake different ecological is repaired the method for means, it is characterized in that the typical submerged plant that step (3) restoration of the ecosystem is selected for use is a waterweed
Elodea nuttallii, black algae
Hydrilla verticilla, eel grass
Vallisneria natans, little tooth Herba potamogetonis distincti
P.maackianus
7. simulation according to claim 4 lake different ecological is repaired the method for means, it is characterized in that the nitrogen cycle bacterium is ammonia oxidizing bacteria, nitrobacteria, nitrite bacteria, denitrifying bacterium.
8. simulation according to claim 4 lake different ecological is repaired the method for means, the application in the restoration of the ecosystem of lake or river drift or submerged plant or nitrogen cycle bacterium in position.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100045051A CN102101725B (en) | 2011-01-11 | 2011-01-11 | Multifunctional lake ecology restoration simulation device and use method as well as application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100045051A CN102101725B (en) | 2011-01-11 | 2011-01-11 | Multifunctional lake ecology restoration simulation device and use method as well as application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102101725A true CN102101725A (en) | 2011-06-22 |
| CN102101725B CN102101725B (en) | 2012-05-02 |
Family
ID=44154816
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100045051A Expired - Fee Related CN102101725B (en) | 2011-01-11 | 2011-01-11 | Multifunctional lake ecology restoration simulation device and use method as well as application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102101725B (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539635A (en) * | 2012-01-05 | 2012-07-04 | 中国农业大学 | Simulating and monitoring device for health status of wetland water ecological system |
| CN102590466A (en) * | 2012-02-16 | 2012-07-18 | 北京师范大学 | Analog device for measuring geochemical process action of river sediment-water micro-interface |
| CN102879441A (en) * | 2012-08-20 | 2013-01-16 | 环境保护部南京环境科学研究所 | Method for determining dissolved oxygen in wetland plant rhizosphere micro-interface in situ |
| CN102937637A (en) * | 2012-10-22 | 2013-02-20 | 中国环境科学研究院 | Water body experiment apparatus and method |
| CN104297444A (en) * | 2014-10-31 | 2015-01-21 | 中国环境科学研究院 | Experimental device for influence of particulate matter variation on water environment |
| CN104374896A (en) * | 2014-11-18 | 2015-02-25 | 天津大学 | Testing system simulating bottom sludge pollution in-situ remediation |
| CN104777271A (en) * | 2015-04-01 | 2015-07-15 | 裴丽欣 | Multifunctional dry and wet deposition trickling filtration testing system |
| CN105523636A (en) * | 2015-12-21 | 2016-04-27 | 浙江省水利河口研究院 | Plant screening and configuration method for improving operation effect of river and lake water body in-situ remediation system |
| CN105585129A (en) * | 2016-01-12 | 2016-05-18 | 南京大学 | Device and method for simulating fate of nitrogen in in-situ river channel ecosystem |
| CN107727833A (en) * | 2017-11-15 | 2018-02-23 | 辽宁大学 | A kind of analogy method and analogue means of multi-functional slope of stope restoration of the ecosystem |
| CN112305164A (en) * | 2020-10-22 | 2021-02-02 | 中国市政工程华北设计研究总院有限公司 | Method for measuring water body restoration capacity of aquatic plant and multifunctional dynamic device |
| CN112479384A (en) * | 2020-11-10 | 2021-03-12 | 中交第三航务工程局有限公司 | Indoor test process for purifying water quality by submerged plants |
| CN113159626A (en) * | 2021-05-13 | 2021-07-23 | 广东河海生态环境技术有限公司 | Submerged plant-complex flora combined immobilized microorganism technology |
| CN114590905A (en) * | 2022-04-27 | 2022-06-07 | 淮北师范大学 | Integrated constructed wetland wastewater treatment system |
| CN114946560A (en) * | 2022-06-13 | 2022-08-30 | 云南大学 | Method for evaluating adaptability of submerged plant to water exchange uniformity and application |
| CN116338131A (en) * | 2023-05-23 | 2023-06-27 | 中交第一航务工程局有限公司 | Test device and method for simulating microbial water quality restoration under different hydrodynamic conditions |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4884892A (en) * | 1987-05-27 | 1989-12-05 | Hydro Data, Inc. | Method and apparatus to generate precisely-defined wall shearing stresses |
| CN1563928A (en) * | 2004-03-16 | 2005-01-12 | 中国科学院南京地理与湖泊研究所 | Method and appts. for indoor simulating refloating state of underwater deposition |
| CN201110849Y (en) * | 2007-10-19 | 2008-09-03 | 中国科学院南京地理与湖泊研究所 | Indoor simulating device used for lake sediment-water interface process research |
| CN201352216Y (en) * | 2008-12-23 | 2009-11-25 | 浙江大学 | Integrated system for sediment sampling and stratification gradient research |
-
2011
- 2011-01-11 CN CN2011100045051A patent/CN102101725B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4884892A (en) * | 1987-05-27 | 1989-12-05 | Hydro Data, Inc. | Method and apparatus to generate precisely-defined wall shearing stresses |
| CN1563928A (en) * | 2004-03-16 | 2005-01-12 | 中国科学院南京地理与湖泊研究所 | Method and appts. for indoor simulating refloating state of underwater deposition |
| CN201110849Y (en) * | 2007-10-19 | 2008-09-03 | 中国科学院南京地理与湖泊研究所 | Indoor simulating device used for lake sediment-water interface process research |
| CN201352216Y (en) * | 2008-12-23 | 2009-11-25 | 浙江大学 | Integrated system for sediment sampling and stratification gradient research |
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539635A (en) * | 2012-01-05 | 2012-07-04 | 中国农业大学 | Simulating and monitoring device for health status of wetland water ecological system |
| CN102539635B (en) * | 2012-01-05 | 2014-09-17 | 中国农业大学 | Simulating and monitoring device for health status of wetland water ecological system |
| CN102590466A (en) * | 2012-02-16 | 2012-07-18 | 北京师范大学 | Analog device for measuring geochemical process action of river sediment-water micro-interface |
| CN102879441A (en) * | 2012-08-20 | 2013-01-16 | 环境保护部南京环境科学研究所 | Method for determining dissolved oxygen in wetland plant rhizosphere micro-interface in situ |
| CN102937637A (en) * | 2012-10-22 | 2013-02-20 | 中国环境科学研究院 | Water body experiment apparatus and method |
| CN102937637B (en) * | 2012-10-22 | 2015-04-15 | 中国环境科学研究院 | Water body experiment apparatus and method |
| CN104297444A (en) * | 2014-10-31 | 2015-01-21 | 中国环境科学研究院 | Experimental device for influence of particulate matter variation on water environment |
| CN104374896A (en) * | 2014-11-18 | 2015-02-25 | 天津大学 | Testing system simulating bottom sludge pollution in-situ remediation |
| CN104777271A (en) * | 2015-04-01 | 2015-07-15 | 裴丽欣 | Multifunctional dry and wet deposition trickling filtration testing system |
| CN105523636A (en) * | 2015-12-21 | 2016-04-27 | 浙江省水利河口研究院 | Plant screening and configuration method for improving operation effect of river and lake water body in-situ remediation system |
| CN105585129A (en) * | 2016-01-12 | 2016-05-18 | 南京大学 | Device and method for simulating fate of nitrogen in in-situ river channel ecosystem |
| CN105585129B (en) * | 2016-01-12 | 2018-12-21 | 南京大学 | A kind of simulation original position river channel ecology system nitrogen returns the device and method to become |
| CN107727833A (en) * | 2017-11-15 | 2018-02-23 | 辽宁大学 | A kind of analogy method and analogue means of multi-functional slope of stope restoration of the ecosystem |
| CN107727833B (en) * | 2017-11-15 | 2023-07-28 | 辽宁大学 | Simulation method and simulation device for ecological restoration of multifunctional stope slope |
| CN112305164A (en) * | 2020-10-22 | 2021-02-02 | 中国市政工程华北设计研究总院有限公司 | Method for measuring water body restoration capacity of aquatic plant and multifunctional dynamic device |
| CN112479384A (en) * | 2020-11-10 | 2021-03-12 | 中交第三航务工程局有限公司 | Indoor test process for purifying water quality by submerged plants |
| CN113159626A (en) * | 2021-05-13 | 2021-07-23 | 广东河海生态环境技术有限公司 | Submerged plant-complex flora combined immobilized microorganism technology |
| CN113159626B (en) * | 2021-05-13 | 2021-11-26 | 广东河海生态环境有限公司 | Submerged plant-complex flora combined immobilized microorganism system |
| CN114590905A (en) * | 2022-04-27 | 2022-06-07 | 淮北师范大学 | Integrated constructed wetland wastewater treatment system |
| CN114946560A (en) * | 2022-06-13 | 2022-08-30 | 云南大学 | Method for evaluating adaptability of submerged plant to water exchange uniformity and application |
| CN116338131A (en) * | 2023-05-23 | 2023-06-27 | 中交第一航务工程局有限公司 | Test device and method for simulating microbial water quality restoration under different hydrodynamic conditions |
| CN116338131B (en) * | 2023-05-23 | 2023-08-22 | 中交第一航务工程局有限公司 | Test device and method for simulating microbial water quality restoration under different hydrodynamic conditions |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102101725B (en) | 2012-05-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102101725B (en) | Multifunctional lake ecology restoration simulation device and use method as well as application | |
| Wang et al. | Effect of a strengthened ecological floating bed on the purification of urban landscape water supplied with reclaimed water | |
| Randrianarison et al. | Microalgae: a potential plant for energy production | |
| CN104326558B (en) | Simulation original position river bottom mud anaerobic ammonium oxidation process device and using method and application | |
| Cao et al. | Removal of nitrogenous compounds from polluted river water by floating constructed wetlands using rice straw and ceramsite as substrates under low temperature conditions | |
| CN101445309B (en) | Ecological fish breeding installation with purified recirculated water | |
| Hua et al. | Effects of plants and temperature on nitrogen removal and microbiology in pilot-scale horizontal subsurface flow constructed wetlands treating domestic wastewater | |
| CN102507913B (en) | Precise quantization method for nitrogen cycle of lake ecosystem | |
| CN103979736B (en) | The constructed wetland device of low-pollution water denitrogenation and treatment process thereof | |
| Viruela et al. | Water resource recovery by means of microalgae cultivation in outdoor photobioreactors using the effluent from an anaerobic membrane bioreactor fed with pre-treated sewage | |
| CN103466879B (en) | In situ purification system for city channel water body | |
| Dong et al. | Diurnal fluctuations in root oxygen release rate and dissolved oxygen budget in wetland mesocosm | |
| Fallowfield et al. | The photosynthetic treatment of pig slurry in temperate climatic conditions: a pilot-plant study | |
| Su et al. | The long-term effects of wall attached microalgal biofilm on algae-based wastewater treatment | |
| CN108911153A (en) | A kind of ecological restoring method of eutrophication water | |
| Takabe et al. | Effects of hydraulic retention time on cultivation of indigenous microalgae as a renewable energy source using secondary effluent | |
| Zhao et al. | Can cold-season macrophytes at the senescence stage improve nitrogen removal in integrated constructed wetland systems treating low carbon/nitrogen effluent? | |
| Zhao et al. | Combined use of cold-season and warm-season macrophytes in floating constructed wetlands to increase nitrogen removal in the early cold season | |
| Feng et al. | A novel plant-girdling study in constructed wetland microcosms: insight into the role of plants in oxygen and greenhouse gas transport | |
| Fu et al. | Transformation of N and S pollutants and characterization of microbial communities in constructed wetlands with Vallisneria natans | |
| Han et al. | Cycling of iodine by microalgae: Iodine uptake and release by a microalgae biofilm in a groundwater holding pond | |
| Yu et al. | Increasing chemical oxygen demand and nitrogen removal efficiencies of surface-flow constructed wetlands in macrophyte-dominant seasons by adding artificial macrophytes | |
| Trebuch et al. | Enhancing phosphorus removal of photogranules by incorporating polyphosphate accumulating organisms | |
| Kim et al. | Nutrient removal from sewage by an artificial food web system composed of phytoplankton and Daphnia magna | |
| CN202279760U (en) | Tree-purification domestic sewage treatment system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120502 Termination date: 20150111 |
|
| EXPY | Termination of patent right or utility model |