CN217337186U

CN217337186U - Cell cryopreservation bag for preparing porcine dendritic cells

Info

Publication number: CN217337186U
Application number: CN202221149070.XU
Authority: CN
Inventors: 朱睿; 张蕾; 卢会鹏
Original assignee: Jiangsu Agri Animal Husbandry Vocational College
Current assignee: Jiangsu Agri Animal Husbandry Vocational College
Priority date: 2022-05-13
Filing date: 2022-05-13
Publication date: 2022-09-02
Anticipated expiration: 2032-05-13

Abstract

The utility model discloses a pig dendritic cell preparation is with cell cryopreserved bag relates to medical instrument technical field, deposit the bag body including freezing, the first place check of the inside one side fixedly connected with of bag body is deposited to freezing, the inside opposite side fixedly connected with second place check of bag body is deposited to freezing, the inside of first place check and second place check all is provided with protection mechanism, the bottom of first place check and second place check all communicates there is the transfer line. The utility model discloses a set up and freeze bag body, first place check, second place check, buffer layer, protective layer and heat preservation, utilize buffer layer, protective layer and heat preservation can effectually play the effect that the inside cell of protection cryopreserved bag body, avoid because the condition that the cell necrosis appears when the cell cryopreserved, thereby cause the damage of cell to lead to the condition that the cell can't be used, be favorable to improving the use of cell, improve the security that the cell deposited the in-process simultaneously.

Description

Cell cryopreservation bag for preparing porcine dendritic cells

Technical Field

The utility model relates to the technical field of medical equipment, especially, relate to a pig dendritic cell preparation is with cell cryopreserved bag.

Background

The cell freezing is one of the main methods for cell preservation, and the freezing technology is utilized to place the cells in liquid nitrogen at the temperature of-196 ℃ for low-temperature preservation, so that the cells can be temporarily separated from the growth state and the cell characteristics can be preserved, and the cells are revived for experiments when needed. Moreover, a certain amount of cells are preserved appropriately, so that the cells can be prevented from being lost due to contamination or other accidents of the cultured cells, and the function of preserving the cells is achieved.

When the existing cell cryopreservation bag is used, the safety of liquid stored in the cryopreservation bag can not be guaranteed, the cell necrosis is easy to occur when the cells are cryopreserved, the damage of the cells is caused, the cell can not be used, the working efficiency of the cell cryopreservation is reduced, the infusion tube is not fixed, the cell omission condition is caused in the cryopreservation process, the cell cryopreservation bag is unstable, and the utilization rate of the cell cryopreservation bag is reduced.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a cell cryopreservation bag for preparing pig dendritic cells.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a cell cryopreservation bag for preparing dendritic cells of pigs comprises a cryopreservation bag body, wherein a first placing lattice is fixedly connected to one side inside the cryopreservation bag body, a second placing lattice is fixedly connected to the other side inside the cryopreservation bag body, and protection mechanisms are arranged inside the first placing lattice and the second placing lattice;

the bottoms of the first placing lattice and the second placing lattice are communicated with a liquid conveying pipe, a placing block is fixedly connected to the bottom of the outer surface of the liquid conveying pipe, a first connecting rod is hinged to one end of the placing block, a second connecting rod is hinged to the other end of the placing block, and a positioning mechanism is connected to the bottom of the first connecting rod;

the bottom of second connecting rod is connected with the second connecting block, one side fixedly connected with stopper of second connecting block, the internal surface fixedly connected with rubber block of stopper.

Preferably, protection mechanism includes the buffer layer of fixed connection in first place check and second place check are inside, the inside of first place check and second place check is located one side fixedly connected with protective layer of buffer layer, the inside of first place check and second place check is located one side fixedly connected with heat preservation of protective layer, the top intercommunication of the lateral wall of first place check and second place check has the leakage fluid dram.

Preferably, the bottom of the first connecting rod is connected with a first connecting block, the side wall of the first connecting block is fixedly connected with a clamping block, the two sides inside the clamping block are fixedly connected with springs, and one end of each spring is fixedly connected with a positioning block.

Preferably, a limiting groove is formed in the clamping block, and the size of the rubber block is matched with the size of the limiting groove formed in the clamping block.

Preferably, the inside of stopper is seted up flutedly, the constant head tank has all been seted up to the inside both sides of recess, the size of fixture block and the inside size looks adaptation of seting up the recess of stopper, the size of locating piece and the inside both sides of recess are seted up the size looks adaptation of constant head tank.

Preferably, the buffer layer is made of a polyurethane material, the protective layer is made of a polypropylene material, and the insulation layer is made of a polylactic acid material.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the buffer layer, the protective layer and the heat preservation layer can effectively protect cells in the cryopreservation bag body, so that the condition that the cells cannot be used due to damage of the cells caused by necrosis of the cells during cryopreservation is avoided, the use of the cells is improved, and meanwhile, the safety in the cell storage process is improved;

2. satisfy through the setting that utilizes fixture block and rubber block and play fixed spacing effect to the transfer line, avoid because the condition that the cell was omitted appears freezing the in-process, there is the instability when leading to the cell to freeze and deposit, be favorable to improving the rate of utilization of cell freezing bag.

Drawings

Fig. 1 is a schematic three-dimensional structure diagram of a cell cryopreservation bag for preparing porcine dendritic cells according to the present invention;

fig. 2 is a schematic view of the internal structure of the cryopreservation bag body of the cell cryopreservation bag for preparing the dendritic cells of the pigs, which is provided by the utility model;

fig. 3 is the utility model provides a pig dendritic cell preparation is with cell cryopreserving bag's connecting block, head rod, second connecting rod, first connecting block and second connecting block connection relation sketch map.

In the figure: 1. freezing and storing the bag body; 2. a first placing grid; 3. a second placing grid; 4. a liquid discharge port; 5. a buffer layer; 6. a protective layer; 7. a heat-insulating layer; 8. a transfusion tube; 9. placing the blocks; 10. a first connecting rod; 11. a first connection block; 12. a clamping block; 13. a spring; 14. positioning a block; 15. a second connecting rod; 16. a second connecting block; 17. a limiting block; 18. a rubber block.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-3, a cell cryopreservation bag for preparing dendritic cells of pigs comprises a cryopreservation bag body 1, wherein a first placing lattice 2 is fixedly connected to one side inside the cryopreservation bag body 1, a second placing lattice 3 is fixedly connected to the other side inside the cryopreservation bag body 1, and protection mechanisms are arranged inside the first placing lattice 2 and the second placing lattice 3;

the bottoms of the first placing lattices 2 and the second placing lattices 3 are both communicated with a transfusion tube 8, the bottom of the outer surface of the transfusion tube 8 is fixedly connected with a placing block 9, one end of the placing block 9 is hinged with a first connecting rod 10, the other end of the placing block 9 is hinged with a second connecting rod 15, and the bottom of the first connecting rod 10 is connected with a positioning mechanism;

the bottom of the second connecting rod 15 is connected with a second connecting block 16, one side of the second connecting block 16 is fixedly connected with a limiting block 17, and the inner surface of the limiting block 17 is fixedly connected with a rubber block 18;

the setting of first place check 2 and second place check 3 satisfies the effect of depositing different cells, utilizes setting up of protection mechanism to have improved the security that cryopreserved bag body 1 deposits the in-process afterwards, utilizes setting up of leakage fluid dram 4 and transfer line 8 to satisfy the entering and the discharge of cell simultaneously, utilizes setting up of placing piece 9 and positioning mechanism to satisfy the effect that plays the joint to transfer line 8 simultaneously, satisfies and deposits the safety and the stability of in-process to cryopreserved bag body 1.

Referring to fig. 1-2, protection mechanism includes the buffer layer 5 of fixed connection inside first place check 2 and second place check 3, the inside of first place check 2 and second place check 3 is located one side fixedly connected with protective layer 6 of buffer layer 5, the inside of first place check 2 and second place check 3 is located one side fixedly connected with heat preservation 7 of protective layer 6, the top intercommunication of the lateral wall of first place check 2 and second place check 3 has leakage fluid dram 4, satisfies the stability of depositing the in-process to freezing bag body 1 through the setting up of buffer layer 5, protective layer 6 and heat preservation 7.

Referring to fig. 1 and 3, the bottom of the first connecting rod 10 is connected with a first connecting block 11, a clamping block 12 is fixedly connected to the side wall of the first connecting block 11, springs 13 are fixedly connected to both sides of the inside of the clamping block 12, a positioning block 14 is fixedly connected to one end of each spring 13, and the springs 13 are extruded to enter the inside of the clamping block 12 after the positioning blocks 14 are pressed, so that the connecting effect between the first connecting block 11 and the second connecting block 16 is satisfied.

Referring to fig. 1 and 3, a limiting groove is formed in the fixture block 12, the size of the rubber block 18 is matched with the size of the limiting groove formed in the fixture block 12, and the limiting groove is formed in the rubber block 18 to extrude the infusion tube 8 and then enter the fixture block 12, so that the clamping effect on the infusion tube 8 is met, and the leakage situation is avoided.

Referring to fig. 1 and 3, the inside of stopper 17 has seted up the recess, the constant head tank has all been seted up to the inside both sides of recess, the size of fixture block 12 and the inside size looks adaptation of seting up the recess of stopper 17, the size of locating piece 14 and the inside both sides of recess set up the size looks adaptation of constant head tank, and the setting of recess satisfies being connected between fixture block 12 and the stopper 17, and being provided with of constant head tank does benefit to the effect that plays the location to locating piece 14 to satisfy the stability of being connected between first connecting block 11 and the second connecting block 16.

Referring to fig. 1-2, the buffer layer 5 is made of a polyurethane material, the polyurethane material has good elasticity, the protective layer 6 is made of a polypropylene material, the polypropylene has high crystallinity and a regular structure, so that the polypropylene has excellent mechanical properties and good chemical stability, the heat insulation layer 7 is made of a polylactic acid material, and the polylactic acid has good thermal stability and certain antibacterial property, flame resistance and ultraviolet resistance, and is beneficial to improving the safety of cells in the process of placing.

The utility model discloses in, during the use, the cell that will freeze and deposit passes through transfer line 8 and carries to the inside of freezing and depositing bag body 1, press extrusion spring 13 after 14 locating pieces to enter into the inside of first connecting block 11 afterwards, press first connecting block 11 and drive the inside that fixture block 12 entered into stopper 17 after 16 second connecting blocks after, loosen afterwards to push back that spring 13 was pressed to locating piece 14 and drive locating piece 14 and pop out from first connecting block 11, thereby enter into the inside of stopper 17, satisfy the connection of first connecting block 11 and second connecting block 16, utilize rubber block 18 to play the effect of keeping off to transfer line 8, avoid freezing and depositing bag body 1 and depositing the condition that the cell was missed in-process appearance.

When freezing bag body 1 cryopreserving in-process, utilize setting up of buffer layer 5, protective layer 6 and heat preservation 7 to improve the security that freezing bag body 1 deposited the in-process, avoid external factors to lead to freezing bag body 1 to deposit the condition that the in-process caused cell necrosis to be favorable to improving the security that the cell freezes and deposits the in-process.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The cell cryopreservation bag for preparing the dendritic cells of the pigs comprises a cryopreservation bag body (1), and is characterized in that a first placing lattice (2) is fixedly connected to one side inside the cryopreservation bag body (1), a second placing lattice (3) is fixedly connected to the other side inside the cryopreservation bag body (1), and protection mechanisms are arranged inside the first placing lattice (2) and the second placing lattice (3);

the bottom parts of the first placing lattice (2) and the second placing lattice (3) are communicated with a liquid conveying pipe (8), the bottom part of the outer surface of the liquid conveying pipe (8) is fixedly connected with a placing block (9), one end of the placing block (9) is hinged with a first connecting rod (10), the other end of the placing block (9) is hinged with a second connecting rod (15), and the bottom part of the first connecting rod (10) is connected with a positioning mechanism;

the bottom of second connecting rod (15) is connected with second connecting block (16), one side fixedly connected with stopper (17) of second connecting block (16), the internal surface fixed connection of stopper (17) has block rubber (18).

2. The cell cryopreservation bag for preparing the dendritic cells of the pigs according to claim 1, wherein the protection mechanism comprises a buffer layer (5) fixedly connected to the inside of the first placing grid (2) and the second placing grid (3), a protection layer (6) is fixedly connected to one side of the buffer layer (5) in the inside of the first placing grid (2) and the second placing grid (3), a heat preservation layer (7) is fixedly connected to one side of the protection layer (6) in the inside of the first placing grid (2) and the second placing grid (3), and a liquid discharge port (4) is communicated with the top of the side wall of the first placing grid (2) and the top of the side wall of the second placing grid (3).

3. The cell cryopreservation bag for preparing the pig dendritic cells, as claimed in claim 1, wherein a first connecting block (11) is connected to the bottom of the first connecting rod (10), a clamping block (12) is fixedly connected to the side wall of the first connecting block (11), a spring (13) is fixedly connected to both sides of the inside of the clamping block (12), and a positioning block (14) is fixedly connected to one end of the spring (13).

4. The cell cryopreservation bag for preparing the dendritic cells of the pigs as claimed in claim 3, wherein a limiting groove is formed in the fixture block (12), and the size of the rubber block (18) is matched with the size of the limiting groove formed in the fixture block (12).

5. The cell cryopreservation bag for preparing the pig dendritic cells, as claimed in claim 3, wherein a groove is formed in the limiting block (17), positioning grooves are formed in both sides of the inner part of the groove, the size of the fixture block (12) is matched with the size of the groove formed in the limiting block (17), and the size of the positioning block (14) is matched with the size of the positioning grooves formed in both sides of the inner part of the groove.

6. The cell cryopreservation bag for preparing porcine dendritic cells according to claim 2, wherein the buffer layer (5) is made of a polyurethane material, the protective layer (6) is made of a polypropylene material, and the heat-insulating layer (7) is made of a polylactic acid material.