CN216637206U

CN216637206U - Memory for remotely storing and transporting a small amount of biological cell samples

Info

Publication number: CN216637206U
Application number: CN202220104423.8U
Authority: CN
Inventors: 向昕怡; 刘敏敏; 徐佳
Original assignee: Nanjing Water Supply And Drainage Engineering Design Institute Co ltd
Current assignee: Nanjing Water Supply And Drainage Engineering Design Institute Co ltd
Priority date: 2022-01-14
Filing date: 2022-01-14
Publication date: 2022-05-31
Anticipated expiration: 2032-01-14

Abstract

The utility model relates to a memory of a small amount of biological cell samples of long-range storage transportation, it relates to biological sample storage field, and it includes outer jar and the inner tank that is used for depositing a plurality of cryovials of placing biological cell samples, the inner tank sets up in the outer jar, can dismantle the connection on the outer jar and be equipped with the enclosing cover, sealing connection is equipped with the inner cup on the inner tank, enclose between outer jar and the inner tank and close the storage space that forms and be used for depositing the refrigerant, the inner cup with be equipped with coupling assembling between the enclosing cover. The biological cell sample crystallization device has the effect of avoiding the crystallization damage of the biological cell sample caused by the sudden cooling of the biological cell sample when the biological cell sample is transported for a long distance as much as possible.

Description

Memory for remotely storing and transporting a small amount of biological cell samples

Technical Field

The present application relates to the field of biological sample storage, and more particularly, to a storage for remotely storing and transporting a small amount of biological cell samples.

Background

Cell cryopreservation is one of the main methods for cell preservation. The use of cryopreservation allows cells to be temporarily removed from growth and their cellular characteristics preserved, thus allowing them to be revived for testing when needed.

The method comprises the steps of placing dry ice in a foam box body, and then placing a freezing pipe filled with the biological cell sample in the dry ice, wherein the freezing pipe and the biological cell sample are cooled suddenly by the method, so that the biological cell sample is crystallized, and the biological cell sample is damaged.

SUMMERY OF THE UTILITY MODEL

In order to improve the problem that the biological cell sample is damaged by crystallization due to the fact that the biological cell sample is cooled suddenly when the biological cell sample is transported for a long distance, the application provides a storage for storing and transporting a small amount of biological cell sample remotely.

The memory for remotely storing and transporting a small amount of biological cell samples adopts the following technical scheme:

the utility model provides a long-range storage memory of transporting a small amount of biological cell samples, includes outer jar and is used for depositing a plurality of inner tanks of placing the cryovial of biological cell samples, the inner tank sets up in the outer jar, can dismantle the connection on the outer jar and be equipped with the enclosing cover, sealing connection is equipped with the inner cup on the inner tank, enclose between outer jar and the inner tank and close the space of depositing that forms and be used for depositing the refrigerant, the inner cup with be equipped with coupling assembling between the enclosing cover.

Through adopting above-mentioned technical scheme, when transporting biological cell sample, at first utilize the inner tank to collect the cryovial of placing biological cell sample, carry out primary cooling with inner tank and cryovial, then pour into the coolant into the parking space in, inner tank and inner cup sealing connection, connect through coupling assembling between inner cup and the enclosing cover, at this moment, put into the outer jar with the inner tank in, enclosing cover and outer jar are connected, carry out the secondary cooling to inner tank and cryovial through the coolant, in the transportation, take place the heat exchange through taking place between the coolant in inner tank and the outer jar, keep the temperature of biological cell sample in the cryovial, avoid as far as possible because rapid cooling makes biological cell sample receive the cold expansion to lead to pushing up the cryovial and the problem that biological cell sample suddenly cools down and lead to biological cell sample crystallization destruction.

In a specific embodiment, coupling assembling includes first magnetic path, second magnetic path, inserted bar, gleitbretter and installation section of thick bamboo, the installation section of thick bamboo with the inner cup orientation the lateral wall fixed connection of enclosing cover, the installation section of thick bamboo orientation seted up on the lateral wall of enclosing cover with the through-hole of the inside intercommunication of installation section of thick bamboo, the inserted bar one end with gleitbretter fixed connection, the gleitbretter is in the inside axis direction motion along the installation section of thick bamboo of installation section of thick bamboo, the other end of inserted bar pass the through-hole and with first magnetic path fixed connection, the second magnetic path with the enclosing cover orientation the lateral wall fixed connection of inner cup, first magnetic path with second magnetic path magnetic adsorption connects.

Through adopting above-mentioned technical scheme, when taking out the inner tank from outer jar, at first demolish the enclosing cover from outer jar, through the pulling enclosing cover, first magnetic path and second magnetic path magnetic adsorption are connected, the second magnetic path drives inserted bar and gleitbretter along the axis direction motion of installation section of thick bamboo, and then takes out inner cover and inner tank from outer jar, the operation of being convenient for, when unfreezing the biological cell sample in the freezing pipe, can unfreeze the inner tube together, avoid biological cell sample at the thawing in-process breakage as far as possible.

In a specific possible embodiment, a metal block is fixedly connected to the side wall of the inner tank facing the bottom of the outer tank.

Through adopting above-mentioned technical scheme, utilize the metal block to accelerate the heat transfer between outer jar interior refrigerant and the inner tank, avoid as far as possible because the temperature rise leads to the biological cell sample in the freezing pipe to become invalid.

In a specific possible implementation scheme, a plurality of supporting seats are arranged on the side wall, facing the bottom of the outer tank, of the inner tank, and a mounting groove is formed in the side wall, facing the supporting seats, of the metal block.

Through adopting above-mentioned technical scheme, when installing the inner tank into the outer jar in, stretch into the mounting groove with the one end that the outer jar tank bottoms was kept away from to the supporting seat in, realized the location to the inner tank, increased the area of contact between the coolant in inner tank lateral wall and the outer jar, improved the cooling effect to the inner tank.

In a specific embodiment, be equipped with cold storage layer in the inner tank, offer the holding tank that is used for placing the cryovial on cold storage layer, be equipped with cold-storage liquid in the cold storage layer.

By adopting the technical scheme, the inner tank which is cooled once and the freezing pipe which is stored with the biological cell sample are continuously cooled and insulated by the cold storage layer, so that the problem of crystallization damage of the biological cell sample during rapid cooling is avoided as much as possible; when the biological cell sample is unfrozen, the freezing pipe is insulated by the cold storage layer filled with cold storage liquid, so that the biological cell sample is prevented from being broken in the unfreezing process due to sudden temperature rise as much as possible.

In a specific practical embodiment, the outer cover is provided with an air outlet, and a pressure stabilizing valve is arranged in the air outlet.

Through adopting above-mentioned technical scheme, utilize the gas vent that is equipped with the surge damping valve to carry out the pressure release to the outer jar, because the refrigerant evaporation leads to the atmospheric pressure increase in the outer jar, avoid the atmospheric pressure in the outer jar as far as possible to cause danger excessively.

In a specific possible embodiment, the outer wall of the outer tank and the side wall of the outer cover far away from the outer tank are both provided with insulating layers.

Through adopting above-mentioned technical scheme, utilize the heat preservation to keep warm to the outer jar, reduce the heat exchange between refrigerant and the air, improved the cold insulation ability to biological cell sample, realized the good storage effect to biological cell sample.

In a specific possible embodiment, the outer vessel is provided with a handle.

Through adopting above-mentioned technical scheme, when transporting biological cell sample, utilize the handle to transport outer jar, avoid as far as possible because the temperature is low excessively to cause the frostbite to operating personnel.

In summary, the present application includes at least one of the following beneficial technical effects:

1. when a biological cell sample is transported, firstly, an inner tank is utilized to collect a freezing pipe with the biological cell sample, the inner tank and the freezing pipe are cooled for the first time, then, a refrigerant is injected into a storage space, the inner tank is connected with the inner cover in a sealing manner, the inner cover is connected with the outer cover through a connecting component, at the moment, the inner tank is placed into an outer tank, the outer cover is connected with the outer tank, the inner tank and the freezing pipe are cooled for the second time through the refrigerant, and in the transportation process, the temperature of the biological cell sample in the freezing pipe is kept through heat exchange between the refrigerants in the inner tank and the outer tank, so that the problems that the freezing pipe is pushed open due to cold expansion of the biological cell sample caused by rapid cooling and the biological cell sample is damaged due to crystallization of the biological cell sample caused by rapid cooling are avoided as much as possible;

2. when taking out the inner tank from outer jar, at first demolish the enclosing cover from outer jar, through the pulling enclosing cover, first magnetic path and second magnetic path magnetic adsorption are connected, and the second magnetic path drives inserted bar and gleitbretter along the axis direction motion of installation section of thick bamboo, and then takes out inner cup and inner tank from outer jar, the operation of being convenient for, when unfreezing the biological cell sample in freezing the pipe, can unfreeze the inner tube together, avoid biological cell sample at the thawing in-process breakage as far as possible.

Drawings

FIG. 1 is a schematic diagram of an overall structure of a storage for remotely storing and transporting a small amount of biological cell samples according to an embodiment of the present application.

Fig. 2 is a cross-sectional view taken along plane a-a of fig. 1.

Fig. 3 is an enlarged view of a portion a in fig. 2.

Description of reference numerals: 1. an outer tank; 2. an inner tank; 3. an outer cover; 4. an inner cover; 5. a freezing pipe; 6. a storage space; 7. a connecting assembly; 71. a first magnetic block; 72. a second magnetic block; 73. inserting a rod; 74. sliding blades; 75. mounting the cylinder; 8. a through hole; 9. a metal block; 10. mounting grooves; 11. a supporting seat; 12. a cold storage layer; 13. a cold storage liquid; 14. accommodating grooves; 15. an exhaust port; 16. a pressure maintaining valve; 17. a handle; 18. and (7) an insulating layer.

Detailed Description

The present application is described in further detail below with reference to figures 1-3.

The embodiment of the application discloses a storage for remotely storing and transporting a small amount of biological cell samples. Referring to fig. 1 and 2, a long-range memory of transporting a small amount of biological cell samples of storage includes outer jar 1 and inner tank 2, outer jar 1 and inner tank 2 are made by the metal, inner tank 2 sets up in outer jar 1, threaded connection is equipped with inner cup 4 on the inner tank 2, threaded connection is equipped with enclosing cover 3 on the outer jar 1, four cryovial 5 that are used for storing the biological cell sample have been placed in the inner tank 2, enclose between outer jar 1 and the inner tank 2 and form the storage space 6 that is used for depositing the refrigerant, in this embodiment, liquid nitrogen or dry ice can be chooseed for use to the refrigerant, be equipped with coupling assembling 7 between enclosing cover 3 and the inner cup 4, when demolising outer cover 3, can take outer jar 1 with inner cup 4 and inner tank 2 together.

When storing a biological cell sample, firstly, the inner tank 2 is used for collecting the freezing pipe 5 with the biological cell sample, the inner cover 4 is screwed on the inner tank 2, then the inner tank 2 and the freezing pipe 5 are cooled for the first time, the temperature is slowly reduced to minus 80 ℃, then liquid nitrogen is injected into the storage space 6, the inner cover 4 and the outer cover 3 are fixedly connected through a connecting component 7, at the moment, the inner tank 2 is arranged in the outer tank 1, the outer cover 3 and the outer tank 1 are fixedly connected through screw threads, through taking place heat exchange between inner tank 2 and the liquid nitrogen in the transportation for inner tank 2 and cryovial 5's temperature further reduces, utilizes the liquid nitrogen in the parking space 6 to carry out the secondary cooling to inner tank 2 and cryovial 5, avoids as far as possible because the temperature sharply reduces and leads to biological cell sample to receive the cold expansion to push away cryovial 5 and biological cell sample suddenly the cooling leads to the problem of crystallization destruction.

Referring to fig. 2 and 3, the connecting assembly 7 includes a first magnetic block 71, a second magnetic block 72, an inserting rod 73, a sliding piece 74 and a mounting cylinder 75, the inserting rod 73, the sliding piece 74 and the mounting cylinder 75 are made of polychlorotrifluoroethylene, so that the inserting rod 73, the sliding piece 74 and the mounting cylinder 75 can normally work in a low temperature environment, the mounting cylinder 75 and the side wall of the inner lid 4 facing the outer lid 3 are fixedly connected, the sliding piece 74 is arranged in the mounting cylinder 75, a through hole 8 is arranged on the side wall of the mounting cylinder 75 close to the outer lid 3, the side wall of the sliding piece 74 far from the inner lid 4 is fixedly connected with the inserting rod 73, one end of the inserting rod 73 far from the sliding piece 74 penetrates through the through hole 8 and is fixedly connected with the first magnetic block 71, the second magnetic block 72 and the side wall of the outer lid 3 facing the inner lid 4 are fixedly connected, and the first magnetic block 71 and the second magnetic block 72 are connected in a magnetic adsorption manner.

When taking out inner tank 2, at first unscrew enclosing cover 3 from outer tank 1, with enclosing cover 3 along outer tank 1's axis direction pulling, enclosing cover 3 drives inserted bar 73 and gleitbretter 74 along outer tank 1's axis direction motion, gleitbretter 74 and installation section of thick bamboo 75 are towards the inner tube wall butt of inner cup 4, and then gleitbretter 74 drives installation section of thick bamboo 75 and inner tank 2 and breaks away from outer tank 1, be convenient for take out biological cell sample from outer tank 1, when the cell sample to in the cryovial 5 unfreezes, can unfreeze inner tank 2 and cryovial 5 together, make the temperature in the cryovial 5 slowly rise, avoid biological cell sample to unfreeze the in-process breakage as far as possible.

Referring to fig. 2, the side wall of the inner tank 2 facing the bottom of the outer tank 1 is fixedly connected with a metal block 9, the metal block 9 has good thermal conductivity, and the metal block 9 is used for accelerating the heat exchange rate between the inner tank 2 and liquid nitrogen, so that a good cooling effect of the inner tank 2 is realized; the side wall of the metal block 9, which is far away from the inner tank 2, is provided with four mounting grooves 10, the inner side wall of the outer tank 1, which faces the inner tank 2, is provided with four supporting seats 11, the supporting seats 11 are in a round table shape, the cross sectional areas of the supporting seats 11 are gradually increased from one end, which is close to the tank opening of the outer tank 1, to one end, which is far away from the tank opening of the outer tank 1, when the inner tank 2 is mounted in the outer tank 1, one end, which is far away from the tank bottom of the outer tank 1, of each mounting seat extends into the mounting groove 10, so that the positioning of the inner tank 2 is realized, and the collision between the inner tank 2 and the outer tank 1 in the transportation process is avoided as much as possible, and further the transportation effect is influenced; utilize the supporting seat 11 of round platform shape to improve the intensity of supporting seat 11, avoid as far as possible that supporting seat 11 becomes fragile under low temperature environment and leads to the fracture, influence inner tank 2's installation stability.

Referring to fig. 2, a cold storage layer 12 is arranged in an inner tank 2, a cold storage liquid 13 is filled in the cold storage layer 12, an accommodating groove 14 for accommodating a freezing pipe 5 is formed in the cold storage layer 12, the cold storage layer 12 and the cold storage liquid 13 are used for heat preservation of a biological cell sample in the freezing pipe 5, when the biological cell sample is cooled for the first time, the biological cell sample is slowly cooled through the cold storage liquid 13, and the biological cell sample is prevented from crystallizing and being broken in the cooling process due to sudden temperature reduction as much as possible; when the biological cell sample is unfrozen, the temperature of the biological cell sample is slowly raised by using the cold storage layer 12 filled with the cold storage liquid 13, and the biological cell sample is prevented from being broken in the unfreezing process due to the sudden temperature rise as much as possible.

Referring to fig. 2, an exhaust port 15 for exhausting is formed in the outer cover 3, a pressure stabilizing valve 16 is arranged in the exhaust port 15, the pressure inside the outer tank 1 is increased due to evaporation of liquid nitrogen in the transportation process, the pressure stabilizing valve 16 is used for relieving the pressure inside the outer tank 1, and the situation that the pressure inside the outer tank 1 is too high and hurts people is avoided as much as possible; the handle 17 is arranged on the outer tank 1, and the handle 17 is used for moving the outer tank 1 to avoid frostbite of operators due to too low temperature as much as possible; all be equipped with heat preservation 18 on the outer wall of outer jar 1 and the outer wall of enclosing cover 3, utilize heat preservation 18 to keep warm to outer jar 1, slow down the heat exchange between outside air and the liquid nitrogen, reduce the quantity of the liquid nitrogen of loss on the road, improved the cold insulation ability to the biological cell sample, realized good storage effect.

The implementation principle of the memory for remotely storing and transporting a small amount of biological cell samples in the embodiment of the application is as follows: when storing the biological cell sample, at first utilize inner tank 2 to collect freezing pipe 5 of placing the biological cell sample, screw up inner cup 4 on inner tank 2, then carry out primary cooling with inner tank 2 and freezing pipe 5, slowly cool down to-80 ℃, at this moment, pour into storage space 6 with liquid nitrogen in, place inner tank 2 in outer tank 1, connect first magnetic path 71 and second magnetic path 72 magnetic adsorption, enclosing cover 3 and outer tank 1 threaded fixation, inserted bar 73 and installation cylinder 75 take place relative rotation, utilize the liquid nitrogen in storage space 6 to carry out the secondary cooling to inner tank 2 and freezing pipe 5, take place heat exchange through between inner tank 2 and the liquid nitrogen in the transportation, make the temperature of inner tank 2 and freezing pipe 5 further reduce, avoid as far as possible because the temperature sharply reduces and lead to the biological cell sample to receive the cold expansion to push up freezing pipe 5 and the problem crystallization that the biological cell sample suddenly makes the destruction of biological cell sample .

After the transportation is accomplished, twist and move enclosing cover 3, when taking off enclosing cover 3 from outer jar 1, inserted bar 73 drives gleitbretter 74 to keeping away from the direction motion of inner cup 4, and then gleitbretter 74 drives installation section of thick bamboo 75 and breaks away from inner tank 2 and outer jar 1, utilizes freezing pipe 5 and inner tank 2 that are equipped with the biological cell sample to unfreeze recovery link together, avoids the result that the sudden change of temperature leads to biological cell sample to destroy as far as possible.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims

1. A storage for remotely storing and transporting a small volume of a biological cell sample, comprising: including outer jar (1) and inner tank (2) that are used for depositing a plurality of cryovials (5) of placing biological cell sample, inner tank (2) set up in outer jar (1), the connection can be dismantled on outer jar (1) and be equipped with enclosing cover (3), sealing connection is equipped with inner cup (4) on inner tank (2), enclose between outer jar (1) and inner tank (2) and close the storage space (6) that form and be used for depositing the refrigerant, inner cup (4) with be equipped with coupling assembling (7) between enclosing cover (3).

2. The storage device of claim 1, wherein the storage device is configured to remotely store and transport a small sample of biological cells, and further comprises: the connecting component (7) comprises a first magnet (71), a second magnet (72), an inserting rod (73), a sliding sheet (74) and an installation barrel (75), the mounting cylinder (75) and the side wall of the inner cover (4) facing the outer cover (3) are fixedly connected, the side wall of the mounting cylinder (75) facing the outer cover (3) is provided with a through hole (8) communicated with the inside of the mounting cylinder (75), one end of the inserted link (73) is fixedly connected with the sliding sheet (74), the sliding sheet (74) moves in the installation cylinder (75) along the axial direction of the installation cylinder (75), the other end of the inserted bar (73) passes through the through hole (8) and is fixedly connected with the first magnetic block (71), the second magnet (72) is fixedly connected with the side wall of the outer cover (3) facing the inner cover (4), the first magnetic block (71) and the second magnetic block (72) are in magnetic adsorption connection.

3. The storage device of claim 1, wherein the storage device is configured to remotely store and transport a small sample of biological cells, and further comprises: the inner tank (2) faces the side wall of the bottom of the outer tank (1) and is fixedly connected with a metal block (9).

4. A storage for remote storage and transportation of small biological cell samples according to claim 3, wherein: inner tank (2) orientation be equipped with a plurality of supporting seats (11) on the lateral wall of outer jar (1) tank bottoms, metal block (9) orientation mounting groove (10) have been seted up on the lateral wall of supporting seat (11).

5. The storage container for remotely storing and transporting small amount of biological cell sample as claimed in claim 1, wherein: be equipped with in inner tank (2) and store up cold layer (12), offer holding tank (14) that are used for placing cryovial (5) on storing up cold layer (12), be equipped with in storing up cold layer (12) and store up cold liquid (13).

6. The storage device of claim 1, wherein the storage device is configured to remotely store and transport a small sample of biological cells, and further comprises: an exhaust port (15) is formed in the outer cover (3), and a pressure stabilizing valve (16) is arranged in the exhaust port (15).

7. The storage device of claim 1, wherein the storage device is configured to remotely store and transport a small sample of biological cells, and further comprises: and the outer wall of the outer tank (1) and the side wall of the outer cover (3) far away from the outer tank (1) are both provided with a heat-insulating layer (18).

8. The storage device of claim 1, wherein the storage device is configured to remotely store and transport a small sample of biological cells, and further comprises: the outer tank (1) is provided with a handle (17).