CN215422477U

CN215422477U - Gradient program cooling device for cell cryopreservation

Info

Publication number: CN215422477U
Application number: CN202121786955.6U
Authority: CN
Inventors: 刘邦正
Original assignee: Shanghai Yiying Biotechnology Co ltd
Current assignee: Shanghai Yiying Biotechnology Co ltd
Priority date: 2021-08-02
Filing date: 2021-08-02
Publication date: 2022-01-07
Anticipated expiration: 2031-08-02

Abstract

The utility model discloses a gradient program cooling device for cell cryopreservation, which comprises a cylindrical storage base, wherein the upper surface of the storage base is provided with a plurality of accommodating holes for accommodating cryopreservation tubes, the accommodating holes comprise first accommodating holes and second accommodating holes, the first accommodating holes are uniformly arranged around the middle part of the storage base at intervals, the second accommodating holes are arranged around the middle part of the storage base and enclose a regular hexagon, and each first accommodating hole is positioned inside the regular hexagon. The accommodating holes on the object placing base are classified and optimally arranged, the plurality of second accommodating holes are made to surround into a regular hexagon, and a plurality of first accommodating holes which are formed into a circular shape are further arranged inside the regular hexagon.

Description

Gradient program cooling device for cell cryopreservation

Technical Field

The utility model relates to a gradient program cooling device for cell cryopreservation.

Background

Cryopreservation refers to a technique for storing biological samples at low temperature (e.g., liquid nitrogen, ultra-low temperature refrigerator). Biological cell samples can be stored in a low-temperature environment for a long time, but if the cells are directly placed in the low-temperature environment, damage such as ice crystals or solutes can be caused to the cells, and the damage is irreversible and can influence the activity of the cells after recovery. The research finds that: the cryoprotectant is added into the frozen sample, and then the frozen sample is directly placed in a low-temperature environment for storage, so that irreversible damage to cells caused by the low-temperature environment can be reduced, but the ideal effect cannot be achieved. Further research shows that the programmed temperature reduction of the cell sample added with the cryoprotectant can obviously reduce the damage of ice crystals or solute and improve the recovery survival rate of cells.

At present, an isopropanol program cooling box or a program cooling instrument is often used for carrying out program cooling on a frozen sample, but isopropanol belongs to a toxic solvent and is extremely easy to volatilize, and long-term use can cause pollution to the environment and harm to physical and psychological health of experimenters. The program cooling instrument is expensive, and has a great risk to the personal safety of experimenters due to the characteristics of volatile liquid nitrogen and extremely low temperature. Moreover, the internal structure design of most program cooling boxes is unreasonable, so that under the condition of the same volume, the space is wasted much, and the accommodated frozen samples are fewer.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the defect that the capacity is smaller due to the unreasonable design of a program cooling box under the condition of the same volume in the prior art, and provides a gradient program cooling device for cell cryopreservation.

The utility model solves the technical problems through the following technical scheme:

the utility model provides a cell freezes deposits and uses gradient procedure heat sink, its is including putting the thing base, it is cylindrical to put the thing base, the upper surface of putting the thing base is equipped with a plurality of holding hole, the holding hole is used for placing the freezing pipe of depositing, the holding hole includes first holding hole and second holding hole, and is a plurality of first holding hole encircles the even interval in middle part of putting the thing base sets up, and is a plurality of second holding hole encircles the middle part of putting the thing base sets up and encloses into regular hexagon, each first holding hole all is located regular hexagon's inside.

In this scheme, to put the holding hole on the thing base and classify and optimize and arrange, make a plurality of second holding holes enclose into regular hexagon, and still be provided with in regular hexagon's inside and enclose into a plurality of first holding holes of circular shape, compare in the mode of arranging of other shapes, but furthest utilizes the cylindrical upper surface space who puts the thing base, can arrange more holding holes, increase the capacity of putting the thing base, improve economic benefits.

Preferably, the accommodating hole further comprises a third accommodating hole, and the third accommodating hole is arranged between each side of the regular hexagon and the edge of the storage base.

In this scheme, the holding hole adopts above-mentioned mode of arranging, but the cylindrical upper surface space who puts the thing base of make full use of to arrange more holding holes, increase the capacity of putting the thing base, improve economic benefits.

Preferably, the gradient procedure cooling device for cell cryopreservation further comprises a box body and a box cover, the storage base is embedded in the box body, the opening of the containing hole faces upwards, and the box cover is arranged on the box body.

In this scheme, the box body will be placed freezing the base of putting of depositing the pipe with the lid and outside isolated, makes the temperature in the box body lower the temperature gradually, also makes freezing the pipe can not directly expose in air conditioning, avoids freezing the cell cooling in depositing the pipe too fast, causes damage such as ice crystal or solute, and then influences the activity after the cell recovery.

Preferably, the upper surface of the box cover is provided with a vent hole communicated with the inner cavity of the box body.

In this scheme, the inner chamber of leading-in box body gradually of outside air conditioning accessible air vent to form gradient cooling effect, place the cooling rate of freezing the pipe in the box body with the control.

Preferably, the center of the placement base is provided with a cold collecting pool, the placement base is not provided with a containing hole at the position of the cold collecting pool, and the projection of the vent hole in the vertical direction is located in the cold collecting pool.

In this scheme, air conditioning forms cascaded procedure cooling effect from the inner chamber of air vent entering box body. Under the action of gravity, a part of cold air descends along the vertical direction to reach the cold accumulating pool and then reaches each freezing pipe through the heat conduction of the cold accumulating pool, so that each freezing pipe placed on the storage base is cooled uniformly; the other part of the cold air forms convection in the inner cavity of the box body and carries out heat exchange and temperature reduction. The cold accumulation pool is an entity of the object placing base, and heat conduction is facilitated.

Preferably, the gradient program cooling device for cell cryopreservation further comprises a vent valve, the vent valve comprises a valve rod and a flange, the flange is connected to one end of the valve rod, the vent valve is provided with a via hole penetrating through the valve rod along the length direction of the valve rod, the flange is embedded in the vent hole, the valve rod extends towards the inner cavity direction of the box body, and the inner cavity of the box body is communicated with the outside through the via hole.

In this scheme, the valve rod of breather valve forms the honeycomb duct, and the honeycomb duct gathers cold pond with outside air conditioning direction more, makes the negative heat in most air conditioning freeze and deposits the pipe through putting thing base heat-conduction diffusion to each, forms balanced cooling effect.

Preferably, the upper surface of the box body is matched with the lower surface seam allowance of the box cover.

In this scheme, adopt above-mentioned structural style, make air conditioning from the air vent of locating the lid upper surface get into the inner chamber of box body as far as possible, reduce outside air conditioning and follow the inside that the faying face between box body and the lid got into the box body.

Preferably, the box body and the box cover are both made of heat insulation materials.

In this scheme, adopt above-mentioned material, prevent that the temperature cooling in the box body is too fast.

Preferably, the base is made of metal.

In this scheme, utilize the good characteristic of metal material heat conduction effect, make and put each freezing and deposit the pipe cooling on the thing base and roughly unanimous.

Preferably, the material of the object placing base is alloy.

On the basis of the common knowledge in the field, the above preferred conditions can be combined randomly to obtain the preferred embodiments of the utility model.

The positive progress effects of the utility model are as follows: the containing holes on the object placing base are classified and optimally arranged, the plurality of second containing holes are made to be surrounded into a regular hexagon, the plurality of first containing holes which are formed into a circular shape are further arranged inside the regular hexagon, and compared with the arrangement mode of other shapes, the cylindrical object placing base can be utilized to the maximum extent, more containing holes can be arranged, the capacity of the object placing base is increased, and the economic benefit is improved.

Drawings

Fig. 1 is a schematic structural view of the placement base installed in the box according to a preferred embodiment of the present invention.

Fig. 2 is a schematic structural diagram of a box body according to a preferred embodiment of the utility model.

Fig. 3 is a schematic structural view of a placement base according to a preferred embodiment of the utility model.

Fig. 4 is a schematic view of a vent valve according to a preferred embodiment of the present invention installed in a first view of a box cover.

Fig. 5 is a schematic view of a vent valve installed in a second view of a box cover according to a preferred embodiment of the present invention.

Fig. 6 is a first perspective view of a vent valve according to a preferred embodiment of the present invention.

Fig. 7 is a second perspective view of the vent valve according to a preferred embodiment of the present invention.

Description of reference numerals:

base for placing articles 1

Cold accumulating tank 11

Accommodation hole 12

First accommodation hole 121

Second accommodation hole 122

Third accommodation hole 123

Box body 2

Box cover 3

Vent hole 31

Breather valve 4

Valve stem 41

Via 411

Flange 42

Detailed Description

The present invention will be more clearly and completely described below by way of examples and with reference to the accompanying drawings, but the present invention is not limited thereto.

As shown in fig. 1 to 7, the present embodiment discloses a gradient program cooling device for cell cryopreservation, which includes a placement base 1. The storage base 1 is cylindrical, a plurality of containing holes 12 are formed in the upper surface of the storage base 1, and the containing holes 12 are used for containing the freezing storage pipes. The containing holes 12 include first containing holes 121 and second containing holes 122, the first containing holes 121 are uniformly arranged around the middle of the containing base 1 at intervals, the second containing holes 122 are arranged around the middle of the containing base 1 and form a regular hexagon, and each first containing hole 121 is located inside the regular hexagon.

In this embodiment, the accommodating holes 12 on the accommodating base 1 are classified and optimally arranged, so that the second accommodating holes 122 are surrounded to form a regular hexagon, and the first accommodating holes 121 are further arranged inside the regular hexagon to form a circle, so that compared with the arrangement mode of other shapes, the upper surface space of the cylindrical accommodating base 1 can be utilized to the maximum extent, more accommodating holes 12 can be arranged, the capacity of the accommodating base 1 is increased, and the economic benefit is improved.

Further, the containing holes 12 further include third containing holes 123, and two third containing holes 123 are arranged between each side of the regular hexagon and the edge of the storage base 1, so that the upper surface space of the cylindrical storage base 1 is fully utilized, more containing holes 12 are arranged, the capacity of the storage base 1 is increased, and economic benefits are improved.

On this basis, cell cryopreserving still includes box body 2 and lid 3 with gradient procedure heat sink, puts thing base 1 and inlays the opening of locating the inside of box body 2 and holding hole 12 up, and box body 2 is located to lid 3 lid. Box body 2 and lid 3 will be placed freezing the thing base 1 of putting that deposits the pipe and completely cut off with the outside, make the temperature in the box body 2 reduce gradually, also make freezing the pipe can not directly expose in air conditioning, avoid freezing the cell cooling in the pipe of depositing too fast, cause damage such as ice crystal or solute, and then influence the activity after the cell recovery.

Preferably, the upper surface of box lid 3 is provided with the air vent 31 with the inner chamber intercommunication of box body 2, and outside air conditioning accessible air vent 31 leads to the inner chamber of box body 2 gradually to form gradient cooling effect, in order to control the cooling rate of placing freezing the pipe in box body 2.

Furthermore, the center of the placement base 1 is provided with a cold collecting pool 11, the placement base 1 is not provided with a containing hole 12 at the position of the cold collecting pool 11, and the projection of the vent hole 31 along the vertical direction is located in the cold collecting pool 11. The cold air enters the inner cavity of the box body 2 from the vent hole 31 to form a stepped program cooling effect. Under the action of gravity, a part of cold air descends along the vertical direction to reach the condensation pool 11 and then reaches each freezing pipe through the heat conduction of the condensation pool 11, so that each freezing pipe placed on the placing base 1 is cooled; the other part of the cold air forms convection in the inner cavity of the box body 2 and carries out heat exchange and temperature reduction. The cold collecting pool 11 is a solid structure of the object placing base 1, and is convenient for heat conduction.

As shown in fig. 4-7, the gradient temperature programming device for cell cryopreservation further comprises a vent valve 4, the vent valve 4 comprises a valve rod 41 and a flange 42, the flange 42 is connected to one end of the valve rod 41, the vent valve 4 is provided with a through hole 411 which penetrates through the valve rod 41 along the length direction of the valve rod 41, and the through hole 411 communicates the inner cavity of the box body 2 with the outside. The flange 42 is fitted into the vent hole 31, and the stem 41 extends toward the inner cavity of the case 2. Here, the valve rod 41 of breather valve 4 forms the honeycomb duct, and the honeycomb duct gathers cold pond 11 through the via hole 411 direction with outside air conditioning more, avoids air conditioning to disperse in advance before gathering cold pond 11 in the flow direction, makes the negative heat in most air conditioning through putting 1 heat-conduction diffusion to each cryopreserving pipe of thing base, forms balanced cooling effect.

The upper surface of the box body 2 is matched with the lower surface seam allowance of the box cover 3, so that most of cold air enters the inner cavity of the box body 2 from the vent hole 31 arranged on the upper surface of the box cover 3, and the external cold air is reduced from entering the inner part of the box body 2 from the joint surface between the box body 2 and the box cover 3. In this embodiment, an inner seam allowance is arranged on the upper surface of the box body 2, an outer seam allowance is arranged on the lower surface of the box cover 3, and the inner seam allowance is matched with the outer seam allowance, so that the sealing effect is improved.

In this embodiment, the material of box body 2 and lid 3 is thermal-insulated material, prevents that the temperature cooling in the box body 2 is too fast.

In order to make the temperature of each freezing tube on the placing base 1 approximately consistent, the placing base 1 is made of metal, and the characteristic of good heat conduction effect of the metal is utilized. In this embodiment, the placing base 1 is made of an alloy. For example, one of nickel-based alloy, aluminum alloy, stainless steel, copper alloy, titanium alloy, and the like. In other alternative embodiments, the material of the placement base may also be carbon fiber with high thermal conductivity.

When the refrigerator is used, the storage base 1 is installed in the box body 2, the freezing storage pipe is placed in the containing hole 12 in the storage base 1, and the box cover 3 is covered on the box body 2 and is integrally placed in a refrigerator at minus 80 ℃ for storage. The majority of external cold air enters the inner cavity of the box body 2 from the through hole 411 on the vent valve 4, and a small amount of cold air enters the inner cavity of the box body 2 from the joint surface of the box body 2 and the box cover 3, so that the cooling speed of all the freezing pipes placed in the box body 2 is consistent, and the stable cooling speed is kept. The gradient program cooling device for cell cryopreservation basically keeps the cooling speed at minus 1 ℃/min, does not cause damage to cells such as ice crystals or solutes and the like, and improves the recovery survival rate of the cells.

While specific embodiments of the utility model have been described above, it will be appreciated by those skilled in the art that this is by way of example only, and that the scope of the utility model is defined by the appended claims. Various changes and modifications to these embodiments may be made by those skilled in the art without departing from the spirit and scope of the utility model, and these changes and modifications are within the scope of the utility model.

Claims

1. The gradient program cooling device for cell cryopreservation comprises a storage base, wherein the storage base is cylindrical, a plurality of containing holes are formed in the upper surface of the storage base and used for containing cryopreservation tubes, and the gradient program cooling device is characterized in that the containing holes comprise first containing holes and second containing holes, the first containing holes are arranged around the middle of the storage base at even intervals, the second containing holes are arranged around the middle of the storage base and surround a regular hexagon, and each first containing hole is located inside the regular hexagon.

2. The gradient programmed cooling device for cell cryopreservation according to claim 1, wherein the containing holes further comprise third containing holes, and the third containing holes are arranged between each side of the regular hexagon and the edge of the storage base.

3. The gradient program cooling device for cell cryopreservation according to claim 1, further comprising a box body and a box cover, wherein the storage base is embedded in the box body, the opening of the storage hole faces upwards, and the box cover is arranged on the box body.

4. The gradient program cooling device for cell cryopreservation as claimed in claim 3, wherein the upper surface of the box cover is provided with a vent hole communicated with the inner cavity of the box body.

5. The gradient program cooling device for cell cryopreservation according to claim 4, wherein a cold accumulation pool is arranged at the center of the placement base, no containing hole is arranged at the position of the cold accumulation pool on the placement base, and the vertical projection of the vent hole is positioned in the cold accumulation pool.

6. The gradient program cooling device for cell cryopreservation according to claim 5, further comprising a vent valve, wherein the vent valve comprises a valve rod and a flange, the flange is connected to one end of the valve rod, the vent valve is provided with a through hole penetrating through the valve rod along the length direction of the valve rod, the flange is embedded in the vent hole, the valve rod extends towards the inner cavity of the box body, and the inner cavity of the box body is communicated with the outside through the through hole.

7. The gradient program cooling device for cell cryopreservation as claimed in claim 4, wherein the upper surface of the box body is matched with the lower surface seam allowance of the box cover.

8. The gradient program cooling device for cell cryopreservation as claimed in claim 3, wherein the box body and the box cover are both made of heat insulation material.

9. The gradient program cooling device for cell cryopreservation according to any one of claims 1 to 8, wherein the placing base is made of metal.

10. The gradient program cooling device for cell cryopreservation of claim 9, wherein the material of the placing base is an alloy.