CN209660269U - Animal liver cell cryopreservation tube - Google Patents

Animal liver cell cryopreservation tube Download PDF

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Publication number
CN209660269U
CN209660269U CN201821612972.6U CN201821612972U CN209660269U CN 209660269 U CN209660269 U CN 209660269U CN 201821612972 U CN201821612972 U CN 201821612972U CN 209660269 U CN209660269 U CN 209660269U
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China
Prior art keywords
ontology
cryopreservation tube
pedestal
wall
liver cell
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CN201821612972.6U
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Chinese (zh)
Inventor
彭珊
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3D BIOOPTIMA Co Ltd
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3D BIOOPTIMA Co Ltd
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Priority to CN201821612972.6U priority Critical patent/CN209660269U/en
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Abstract

The utility model discloses a kind of animal liver cell cryopreservation tube, the cryopreservation tube is by pedestal, ontology and pipe Gai Zucheng, ontology is mutually threadedly coupled with pipe lid, wherein ontology is the cylindrical tube of open top, and ontology is recessed the hollow posts close to top from bottom upwards, the width exhibition of pedestal is greater than ontology in the projection of bottom side, and pedestal is connected to hollow posts, and cap wall is equipped with the sealing ring for externally completely cutting off the chamber body for being connected to pipe lid.Using the cryopreservation tube of the utility model, the anti-rollover ability of pedestal is increased, sample loss and pollution are avoided;Thoroughly prevent to seep in liquid nitrogen in frozen storage process, has eliminated the risk that the heated explosive of cryopreservation tube causes life to injure;The matching identification for improving cryopreservation tube, can effectively prevent that there is a situation where sample cross contamination appearance;Furthermore the heating surface area for also adding cell liquid in cryopreservation tube shortens the time of cell recovery, improves survival rate.

Description

Animal liver cell cryopreservation tube
Technical field
The utility model relates to the relevant vessel fields of cell culture, more particularly to one kind to be used for toy hepatic cell frozen storing Sealing container.
Background technique
Cell culture technology has become the indispensable research method in natural science field originating from 1907 so far One of, this basic technology is also particularly important the freezen protective of cell with defrosting recovery.Cell cryopreservation is that cell saves Cell is placed in cryo-conservation in -196 DEG C of liquid nitrogen using Cryopreservation Technology, cell can be made to be temporarily disengaged from life by one of main method Long status and its cell characteristics is saved, so when needed again recovery cell for testing.
Current existing cell cryopreservation tube often occurs that leakproofness is poor, and liquid nitrogen easily infiltrates into when freezing, and exists by thermal explosion Risk, and pipe drowning has mark zone, and pipe lid and ontology is caused to be unable to Rapid matching, and service efficiency is low, in addition, commercially available jelly Pipe most of the problem of all there is setting shakiness, being easy rollover is deposited, causes cell liquid to be poured down or forth, pollutes.
Summary of the invention
The purpose of this utility model is directed to a kind of animal liver cell cryopreservation tube, overcomes existing common cryopreservation tube with this Many drawbacks.
The utility model realizes that the technical solution of above-mentioned purpose is animal liver cell cryopreservation tube, it is characterised in that: institute Cryopreservation tube is stated by pedestal, ontology and pipe Gai Zucheng, ontology is mutually threadedly coupled with pipe lid, wherein the ontology is the circle of open top Cylinder, and ontology is recessed the hollow posts close to top from bottom upwards, the width exhibition of the pedestal is greater than ontology the bottom of at The projection of side, and pedestal is connected to hollow posts, the cap wall is equipped with for externally completely cutting off the chamber body for being connected to pipe lid Sealing ring.
Further, ontology top side outer wall is equipped with external screw thread, and pipe base side inner wall is equipped with internal screw thread, the pipe Lid is socketed on bodies top and is fixed by being screwed on for internal and external screw thread.
Further, the body outer wall of the cryopreservation tube is respectively equipped with scale and sample classification marker.
Further, the pipe hd top face of the cryopreservation tube is equipped with and matches with sample classification marker set by body outer wall Label.
Further, the outer wall of the ontology is the corrugated surface wall of cylindrical straight wall or cylinder.
Further, the bottom side contact surface of the pedestal is triangle, and projection of the ontology in bottom side is wrapped in triangle entirely Among.
Further, the bottom side contact surface of the pedestal is circle, and projected area of the ontology in bottom side is less than pedestal bottom Flank the area of contacting surface.
Using the cryopreservation tube of the utility model, have substantive distinguishing features and progressive: the cryopreservation tube is changed by understructure It is good, the anti-rollover ability of pedestal is increased, sample loss and pollution are avoided;By adding sealing ring, thoroughly prevent to freeze It is seeped in liquid nitrogen in the process, eliminates the risk that the heated explosive of cryopreservation tube causes life to injure;Increase information flag by surface, The matching identification for improving cryopreservation tube, can effectively prevent that there is a situation where sample cross contamination appearance;Furthermore originally by improvement Body be it is hollow, increase the heating surface area of cell liquid in cryopreservation tube, shorten the time of cell recovery, improve survival rate.
Detailed description of the invention
Fig. 1 is the Structure explosion diagram of the utility model cryopreservation tube.
Fig. 2 is that the axis of the utility model cryopreservation tube main body cuts open structural schematic diagram.
Fig. 3 is another implementation structural schematic diagram of the utility model cryopreservation tube pedestal.
Specific embodiment
Just attached drawing in conjunction with the embodiments below, is described in further detail specific embodiment of the present utility model, so that this Utility model technical solution is more readily understood, grasps, to make relatively sharp define to the protection scope of the utility model.
In view of many defects for freezing vessel for being currently used for cell culture, the utility model designer is through researching and analysing simultaneously Innovation proposes a kind of novel toy hepatic cell frozen storing pipe, with this realize efficiently, safety, no pollution freeze operation.
As shown in Figure 1 to Figure 3, the general introduction scheme of the animal liver cell cryopreservation tube is, by pedestal 3, ontology 1 and pipe lid 2 Composition, ontology 1 and pipe 2 lid are mutually threadedly coupled, and wherein ontology 1 is the straight wall cylindrical tube of open top, and ontology is from bottom It is recessed the hollow posts 11 close to top upwards, and the exhibition of the width of pedestal is greater than ontology in the projection of bottom side, and pedestal is connected to Hollow posts 11,2 inner wall of pipe lid are equipped with the sealing ring 21 for externally completely cutting off the chamber body for being connected to pipe lid.As originally freezing The above-mentioned essential features of pipe, bodies top opening are the injection and extraction for the ease of culture solution, and the design of middle control column It is intended to increase the surface area of ontology, convenient for quickly transmitting heat when thermo-contact, pedestal amplification is intended to ensure that the cryopreservation tube is established and puts The stability set, overturning-preventing.The sealing ring of pipe lid is intended to prevent liquid nitrogen from penetrating into pipe simultaneously.
In addition to above-mentioned necessary structure, each component part of the cryopreservation tube of the utility model also have further refinement or The design scheme of optimization.Specific details are as follows: the 1 top side outer wall of ontology is equipped with external screw thread 13, which is equipped with interior Screw thread 23, pipe lid 2 is socketed on fixation at the top of ontology 1 by being screwed on for internal and external screw thread as a result,.In addition, the sheet of the cryopreservation tube is external Wall is respectively equipped with scale 14 and sample classification marker 12, and the pipe hd top face of cryopreservation tube is equipped with and sample set by body outer wall point Class marks the label 22 to match;Thus, it is possible to facilitate experimenter to correspond operation, sample room cross contamination is prevented.
From shown in Fig. 1 as it can be seen that the bottom side contact surface of the pedestal be circle, and ontology bottom side projected area be less than pedestal The area of bottom side contact surface.Certainly, be not limited to above embodiment, the bottom side contact surface of pedestal 3a be also possible to triangle or Other regular polygons, and projection of the ontology in bottom side is wrapped in entirely among the contact surface of bottom side, here, triangle and the equal energy of regular polygon Effectively achieve the purpose that overturn-preventing.
In addition to above preferred embodiment, in the other embodiments of the cryopreservation tube, the outer wall of ontology is cylindrical ripple Curved wall.It is possible thereby to further increase surface heating surface area, accelerate the recovery of freeze-stored cell.
To sum up, the detailed description for the structure of the utility model and application method as it can be seen that using the utility model jelly Deposit pipe, have substantive distinguishing features and progressive: 1, the improvement of pedestal increases the stability of cryopreservation tube setting, adds by cell liquid It is fashionable, it avoids crossing senior general's cryopreservation tube because of operating force and overturn, cause sample loss and pollution;2, ontology and pipe Gai Douyou information mark Remember area, facilitates identification, and can guarantee that pipe lid with the one-to-one correspondence of tube body, prevents sample room cross contamination;3, sealing ring increases The leakproofness for having added cryopreservation tube prevents cell when liquid nitrogen container saves, and liquid nitrogen penetrates into pipe (because once having liquid nitrogen infiltration, In When cell recovery operates, cryopreservation tube is heated is easy explosion suddenly, causes personal injury);4, ontology vertical wall section is using hollow Design, when being conducive to cell recovery, increases the heating surface area of cell liquid, shortens the time of cell recovery, improves Cell viability.
The utility model is described in detail above by specific embodiment, which is only the utility model Preferred embodiment, not to the restriction of requested protection scope.In the case where not departing from the utility model principle, ability Any modification, equivalent substitution and improvement that domain those of ordinary skill is done under the premise of without creative work it is obtained its Its embodiment, is regarded as in the technical protection scope of the utility model.

Claims (7)

1. animal liver cell cryopreservation tube, it is characterised in that: the cryopreservation tube is by pedestal, ontology and pipe Gai Zucheng, ontology and Guan Gai It is mutually threadedly coupled, wherein the ontology is the cylindrical tube of open top, and ontology is recessed upwards from bottom close to top The width exhibition of the hollow posts in portion, the pedestal is greater than ontology in the projection of bottom side, and pedestal is connected to hollow posts, the cap wall Equipped with the sealing ring for externally completely cutting off the chamber body for being connected to pipe lid.
2. animal liver cell cryopreservation tube according to claim 1, it is characterised in that: ontology top side outer wall is equipped with outer spiral shell Line, pipe base side inner wall are equipped with internal screw thread, and the pipe lid is socketed on bodies top and is fixed by being screwed on for internal and external screw thread.
3. animal liver cell cryopreservation tube according to claim 1, it is characterised in that: the body outer wall of the cryopreservation tube is set respectively There are scale and sample classification marker.
4. animal liver cell cryopreservation tube according to claim 3, it is characterised in that: the pipe hd top face of the cryopreservation tube be equipped with The label that sample classification marker matches set by body outer wall.
5. animal liver cell cryopreservation tube according to claim 1, it is characterised in that: the outer wall of the ontology is the straight of cylinder The corrugated surface wall of wall or cylinder.
6. animal liver cell cryopreservation tube according to claim 1, it is characterised in that: the bottom side contact surface of the pedestal is triangle Shape, and projection of the ontology in bottom side is wrapped among triangle entirely.
7. animal liver cell cryopreservation tube according to claim 1, it is characterised in that: the bottom side contact surface of the pedestal is circle Shape, and projected area of the ontology in bottom side is less than the area of underside contact surface.
CN201821612972.6U 2018-09-30 2018-09-30 Animal liver cell cryopreservation tube Active CN209660269U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201821612972.6U CN209660269U (en) 2018-09-30 2018-09-30 Animal liver cell cryopreservation tube

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201821612972.6U CN209660269U (en) 2018-09-30 2018-09-30 Animal liver cell cryopreservation tube

Publications (1)

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CN209660269U true CN209660269U (en) 2019-11-22

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CN201821612972.6U Active CN209660269U (en) 2018-09-30 2018-09-30 Animal liver cell cryopreservation tube

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111903666A (en) * 2020-09-03 2020-11-10 浙江省畜牧技术推广与种畜禽监测总站(浙江省农业机械试验鉴定推广总站) Hu sheep semen freezing tube and thawing apparatus
WO2023050537A1 (en) * 2021-10-01 2023-04-06 美东汇成生命科技(昆山)有限公司 High-practicability cryovial

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111903666A (en) * 2020-09-03 2020-11-10 浙江省畜牧技术推广与种畜禽监测总站(浙江省农业机械试验鉴定推广总站) Hu sheep semen freezing tube and thawing apparatus
WO2023050537A1 (en) * 2021-10-01 2023-04-06 美东汇成生命科技(昆山)有限公司 High-practicability cryovial

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