CN201056374Y - Low-temperature pipe for freezing and storing biological organization - Google Patents

Low-temperature pipe for freezing and storing biological organization Download PDF

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Publication number
CN201056374Y
CN201056374Y CNU2007200911931U CN200720091193U CN201056374Y CN 201056374 Y CN201056374 Y CN 201056374Y CN U2007200911931 U CNU2007200911931 U CN U2007200911931U CN 200720091193 U CN200720091193 U CN 200720091193U CN 201056374 Y CN201056374 Y CN 201056374Y
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China
Prior art keywords
tube
biological tissue
pipe
low
cover
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Expired - Fee Related
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CNU2007200911931U
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Chinese (zh)
Inventor
鲍玉洲
张艳敏
魏秋彩
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Individual
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Individual
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Priority to CNU2007200911931U priority Critical patent/CN201056374Y/en
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Publication of CN201056374Y publication Critical patent/CN201056374Y/en
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Expired - Fee Related legal-status Critical Current

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Abstract

The utility model discloses a low temperature biological tissue freezing tube, comprising a centrifugal tube body and a tube cover; wherein a tube wall close to a tube opening of the centrifugal tube is opened with a decompression hole. A connecting belt is arranged between the centrifugal tube body and the tube cover. After the tube cover and the tube body are fastened, the bending part of the connecting belt becomes in the ring-shape. A tube cover locking device is arranged between the tube body and the tube cover. The decompression hole opened on the tube wall not only can be taken as an inlet through which liquid nitrogen is automatically injected into the tube when the tube body is infused with the liquid nitrogen but also can discharge the high pressure generated by the rapid gasification of the liquid nitrogen in the tube and have the function of preventing the tube body from being exploded. The biological tissue does not need to be taken out and can directly be crashed by a grinding rod, and nucleic acid is extracted from the biological tissue; therefore, the unnecessary exogenous gene pollution caused by the method that the gene is moved into a special grinder to be grinded when the gene is extracted is avoided. After becoming the ring-shape, the connecting belt can be used to fix a label or a rope. The tube cover locking device can prevent the tube cover from automatically opening because of chilling shrinkage when the tube cover is frozen and preserved.

Description

Low-temperature biological tissue freezing pipe
Technical field
The utility model relates to the preservation pipe of the biological organic organization of a kind of cryopreservation material.
Background technology
In technical field of molecular biology, be not destroyed in order to make biological organization sample nucleic acid in the tissue samples in long preservation and transportation, adopt the special-purpose pipe of preserving of liquid nitrogen that tissue samples is kept in the liquid nitrogen usually.At present, the special-purpose pipe one side price of preserving of liquid nitrogen on the market is higher, on the other hand after from liquid nitrogen, taking out this preservation pipe, tissue samples can't grind tissue in pipe is preserved in this special use, can only earlier tissue samples be taken out, put into mill and grind, extract nucleic acid again, so not only increase control step and running cost, also increased the approach that pollutes tissue samples.In order to reduce retain costs, utilize common plastic centrifuge tube that tissue samples is kept in the liquid nitrogen, then when from liquid nitrogen, taking out the centrifuge tube of splendid attire sample; centrifuge tube is in the moment of ingress of air; blast through regular meeting, cause tissue samples normally to use, even can damage the operator.In addition, no matter being that liquid nitrogen is special-purpose preserves pipe or plastic centrifuge tube normally is loaded in gauze bag or other can, on gauze bag or other can is again to hang label to show the difference with other samples, very inconvenient in the operation.
The utility model content
The purpose of this utility model provides a kind of low-temperature biological tissue freezing pipe esy to use, safe and reliable.
For achieving the above object, the utility model adopts following technical scheme: low-temperature biological tissue freezing pipe, comprise centrifuge tube body and Guan Gai, and having vent near on the tube wall of the centrifuge tube mouth of pipe.
Be provided with connecting band between centrifuge tube body and the pipe lid, after the pipe lid fastened with body, the crooked position of connecting band formed ring-type.
Also being provided with the pipe cover lock between body and pipe lid tightly installs.
The tight device of described pipe cover lock comprises the locking piece of being located on the body, is provided with locking body with its fastening at Guan Gai edge correspondence position.
Described vent is 3-8, rectangular distribution on same circumference.
The utility model is the improvement of carrying out on the basis of existing centrifuge tube, and the centrifuge tube after the improvement can use as the frozen pipe of liquid nitrogen frozen, has enlarged the Applicable scope of centrifuge tube, greatly reduces the cost of frozen pipe.On the centrifuge tube tube wall, offer vent, not only can be when it be immersed liquid nitrogen as the liquid nitrogen import in the filling tube voluntarily, when from liquid nitrogen, taking out body, can also gasify and the high pressure of generation rapidly as releasing tube internal cause liquid nitrogen, thereby play the effect that prevents the body blast; In addition, vent is opened on the tube wall, can also reduce or avoid to pollute to biological tissue in the containment ingression pipe.This preservation pipe can not only be used for the long-term liquid nitrogen of biological tissue's amplifying nucleic acid (comprising DNA and RNA) and preserve, and be stored in biological tissue in this pipe and just can also need not take out and directly pulverize and extract nucleic acid with grinding rod, can avoid when extracting genetic manipulation because of blasting or gene being moved into the unnecessary allogenic gene pollution that grinding brings in the special-purpose mill.After the connecting band between body and the pipe lid forms ring-type, can be used to fixed labels or rope, the pipe cover lock tightly installs and is used for preventing when frozen opening voluntarily because of the pipe that may cause after the shrinkage covers.The utility model is simple in structure, and is easy to operate, economical and practical.
Description of drawings
Fig. 1 is the structural representation after the utility model fastens the pipe lid;
Fig. 2 is the structural representation of the grinding rod that is used with the utility model;
Fig. 3 is the structural representation that the utility model is opened the pipe lid;
Fig. 4 be among Fig. 3 A to partial view.
The specific embodiment
Fig. 1, Fig. 3, low-temperature biological tissue freezing pipe shown in Figure 4, it comprises that centrifuge tube body 1 and pipe covers 2, are evenly having six vents 3 on the close same circumference in tube wall upper edge of the centrifuge tube mouth of pipe; Centrifuge tube body 1 and pipe covers the connecting band 4 that is provided with certain-length between 2, when pipe covers 2 spiral-locks after on the body 1, the crooked position formation ring-type of connecting band 4; Cover also to be provided with between 2 to prevent to manage at body 1 and pipe and cover the 2 pipe cover locks of opening voluntarily and tightly install, the tight device of this pipe cover lock comprises by connecting band 6 and is connected lock ball 5 on the body 1, and the position of cover 2 the corresponding lock ball 5 in edge at pipe is provided with to compress to manage by fastening lock ball 5 covers 2 pressing groove 7.The utility model is made of plastics.
Shown in Figure 2 is and the matching used grinding rod of centrifuge tube, and the interior shape at the bottom of its grinding head and the centrifuge tube pipe adapts, and the top of grinding head is provided with spot-like projections, skids in the time of can preventing to grind, and improves grinding efficiency.

Claims (6)

1. low-temperature biological tissue freezing pipe comprises centrifuge tube body and Guan Gai, it is characterized in that: having vent near on the tube wall of the centrifuge tube mouth of pipe.
2. low-temperature biological tissue freezing pipe according to claim 1 is characterized in that: be provided with connecting band between centrifuge tube body and the pipe lid, after the pipe lid fastened with body, the crooked position of connecting band formed ring-type.
3. low-temperature biological tissue freezing pipe according to claim 1 and 2 is characterized in that: also be provided with the pipe cover lock and tightly install between body and pipe lid.
4. low-temperature biological tissue freezing pipe according to claim 3 is characterized in that: the tight device of described pipe cover lock comprises the locking piece of being located on the body, is provided with locking body with its fastening at Guan Gai edge correspondence position.
5. low-temperature biological tissue freezing pipe according to claim 3 is characterized in that: described vent is 3-8, rectangular distribution on same circumference.
6. low-temperature biological tissue freezing pipe according to claim 4 is characterized in that: described vent is 3-8, rectangular distribution on same circumference.
CNU2007200911931U 2007-07-25 2007-07-25 Low-temperature pipe for freezing and storing biological organization Expired - Fee Related CN201056374Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNU2007200911931U CN201056374Y (en) 2007-07-25 2007-07-25 Low-temperature pipe for freezing and storing biological organization

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNU2007200911931U CN201056374Y (en) 2007-07-25 2007-07-25 Low-temperature pipe for freezing and storing biological organization

Publications (1)

Publication Number Publication Date
CN201056374Y true CN201056374Y (en) 2008-05-07

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Family Applications (1)

Application Number Title Priority Date Filing Date
CNU2007200911931U Expired - Fee Related CN201056374Y (en) 2007-07-25 2007-07-25 Low-temperature pipe for freezing and storing biological organization

Country Status (1)

Country Link
CN (1) CN201056374Y (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103101866A (en) * 2013-01-22 2013-05-15 刘江 Oil drainage pipeline leakage prevention device for oil storage tank
CN103868752A (en) * 2012-12-12 2014-06-18 江南大学 Rapid biological tissue block preparation and cryopreservation apparatus
CN105289773A (en) * 2015-09-25 2016-02-03 中国农业科学院茶叶研究所 Sample pretreatment method for plant metabolome analysis
CN106119097A (en) * 2016-08-26 2016-11-16 云南中烟工业有限责任公司 A kind of centrifugal frozen dual-purpose explosion stack
CN111099166A (en) * 2020-01-06 2020-05-05 南通大学 Sample holding device suitable for remote sending of transmission electron microscope biological tissue sample

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103868752A (en) * 2012-12-12 2014-06-18 江南大学 Rapid biological tissue block preparation and cryopreservation apparatus
CN103868752B (en) * 2012-12-12 2016-10-05 江南大学 The preparation of quick bio tissue block and cryopreservation device
CN103101866A (en) * 2013-01-22 2013-05-15 刘江 Oil drainage pipeline leakage prevention device for oil storage tank
CN105289773A (en) * 2015-09-25 2016-02-03 中国农业科学院茶叶研究所 Sample pretreatment method for plant metabolome analysis
CN106119097A (en) * 2016-08-26 2016-11-16 云南中烟工业有限责任公司 A kind of centrifugal frozen dual-purpose explosion stack
CN111099166A (en) * 2020-01-06 2020-05-05 南通大学 Sample holding device suitable for remote sending of transmission electron microscope biological tissue sample

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C17 Cessation of patent right
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Granted publication date: 20080507