CN206289239U - DNA of plants rapid extraction corollary apparatus - Google Patents
DNA of plants rapid extraction corollary apparatus Download PDFInfo
- Publication number
- CN206289239U CN206289239U CN201621470683.8U CN201621470683U CN206289239U CN 206289239 U CN206289239 U CN 206289239U CN 201621470683 U CN201621470683 U CN 201621470683U CN 206289239 U CN206289239 U CN 206289239U
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- China
- Prior art keywords
- blade
- pipe
- abrasive disk
- extract
- dna
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn - After Issue
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Abstract
The utility model is related to a kind of plant leaf blade DNA extraction elements, belongs to biological experiment equipment technical field.During the utility model is in order to solve to realize that plant leaf blade DNA extractions are tested at present, plant leaf blade DNA extracts cumbersome, the low problem of extraction efficiency.The utility model includes that blade and extract preserve pipe, lapping device, memotron and the first abrasive disk, the bottom that blade and extract preserve pipe is provided with the first abrasive disk, blade and extract preserve and lapping device are provided with the mouth of pipe of pipe, and storage lid preserves the upper end of pipe mounted in blade and extract.The utility model novel in structural design, using it is easy to operate, be easy to lab assistant carry out plant leaf blade DNA extract experiment use, improve extraction efficiency.
Description
Technical field
The utility model is related to a kind of plant leaf blade DNA extraction elements, belongs to biological experiment equipment technical field.
Background technology
With the development of molecular biology, breeding process can be greatly speeded up using molecular mark technology, improved
Breeding efficiency, and it is the premise and key for carrying out molecule marking research that DNA is extracted.
Existing plant leaf blade DNA is extracted and crushed using instruments such as grinding alms bowls, then carries out water-bath, and fully cracking is thin
After birth and nuclear membrane, make DNA be discharged from cell；Then plus phenol or chloroform deproteinized, aldehydes matter and polysaccharide etc., from
The heart；Supernatant is taken, uses isopropanol precipitating DNA, when plant leaf blade DNA is extracted in this operation, ground the stage in sample, main use is ground
Mill alms bowl is crushed, and is mixed during the plant leaf blade after crushing and corresponding buffer solution then are loaded into unified vessel, then
Water-bath is carried out, in realizing that plant leaf blade DNA extractions are tested at present, due to that can be realized conveniently without corresponding experiments supporting device
The integrated corollary apparatus of the series of process such as grinding, water-bath, centrifugation, cause plant leaf blade DNA to extract cumbersome, extraction efficiency
It is low, and can there is the pollution between certain sample in experimentation.
Utility model content
The purpose of this utility model be in order to solve the above problems, and then provide DNA of plants rapid extraction corollary apparatus.
The technical solution of the utility model：
DNA of plants rapid extraction corollary apparatus include that blade and extract preserve pipe, lapping device, memotron and first and grind
It is cylindrical tube that mill, described blade and extract preserve pipe, and the bottom that blade and extract preserve pipe is provided with first
Abrasive disk, blade and extract to be preserved and be provided with lapping device at the mouth of pipe of pipe, and described lapping device includes abrasive disk control
Bar, fixed lid and control-rod fixed mount, described fixed lid is fixedly mounted on blade and extract is preserved at the mouth of pipe of pipe, fixed
Cover end and control-rod fixed mount is installed, described abrasive disk control-rod may be rotatably mounted on control-rod fixed mount, abrasive disk
Control-rod passes through that fixed lid is arranged on blade and extract is preserved in pipe, and the lower end end of abrasive disk control-rod is provided with and the
The second abrasive disk that one abrasive disk is used cooperatively, the upper end end of abrasive disk control-rod is provided with handle；Described memotron
Lid preserves the upper end of pipe mounted in blade and extract.
Preferably：Isolation lid is installed, isolation is covered and is provided with funnel-form Storage Cup, the Storage Cup in described memotron
Infundibulate edge fitted completely with the inner wall section of memotron.
Preferably：The lateral wall of blade and extract preservation pipe and memotron is provided with label and is placed with mouth.
Preferably：It is raised that the first described abrasive disk is provided with some particulate abrasives.
Preferably：The lower surface of the second described abrasive disk is provided with the raised and some through hole of some particulate abrasives.
The utility model has the advantages that：DNA of plants rapid extraction corollary apparatus of the present utility model, by plant
Grinding operation, water bath containers, centrifuge container and extraction vessel involved by leaf DNA extraction process are integrated, and when using, lead to
The grinding that lapping device preserves the cooperation completion plant leaf blade of pipe with blade and extract is crossed, it passes through manipulation operations abrasive disk control
Bar processed causes that be arranged on the upper and lower abrasive disk of plant leaf blade grinds to its blade, and this operation is simpler, convenient；Grinding bonnet
Upper memotron can directly carry out water-bath, be inverted memotron after water-bath so that leaf tissue be stored in funnel-form storage cup
Buffer solution mixing, you can obtain leaf DNA.The utility model novel in structural design, using it is easy to operate, be easy to people from laboratory
Member carries out the use that plant leaf blade DNA extracts experiment, improves extraction efficiency.
Brief description of the drawings
Fig. 1 is DNA of plants rapid extraction corollary apparatus structure assembling figure；
Fig. 2 is that blade and extract preserve tubular construction figure；
Fig. 3 is lapping device structure chart；
Fig. 4 is storage tubular construction installation diagram；
Fig. 5 is memotron textural association figure；
Fig. 6 is the second abrasive disc structure figure；
1- blades and extract preserve pipe, 2- lapping devices, 3- memotrons, the abrasive disks of 4- first, 5- abrasive disk controls in figure
Bar processed, 6- fixes lid, 7- control-rod fixed mounts, the abrasive disks of 8- second, 9- isolation lids, 10- funnel-form Storage Cups, 11- labels patch
Mouth is put, 12- grindings are raised, 13- through holes, 14- handles.
Specific embodiment
Specific embodiment one：Present embodiment is illustrated with reference to Fig. 1, the DNA of plants rapid extraction of present embodiment is supporting
Device includes that blade and extract preserve pipe 1, lapping device 2, the abrasive disk 4 of memotron 3 and first, described blade and extract
It is cylindrical tube to preserve pipe 1, and the bottom that blade and extract preserve pipe 1 is provided with the first abrasive disk 4, and blade and extract are protected
Lapping device 2 is installed, described lapping device 2 includes abrasive disk control-rod 5, fixed lid 6 and control-rod at the mouth of pipe for depositing pipe 1
Fixed mount 7, described fixed lid 6 is fixedly mounted on blade and extract is preserved at the mouth of pipe of pipe 1, and the fixed upper end of lid 6 is provided with
Control-rod fixed mount 7, described abrasive disk control-rod 5 may be rotatably mounted on control-rod fixed mount 7, and abrasive disk control-rod 5 is worn
Cross fixed lid 6 to be arranged in blade and extract preservation pipe 1, the lower end end of abrasive disk control-rod 5 is provided with grinds with first
The second abrasive disk 8 that mill 4 is used cooperatively, the upper end end of abrasive disk control-rod 5 is provided with handle；Described memotron 3
Lid preserves the upper end of pipe 1 mounted in blade and extract；Isolation lid 9 is installed in described memotron 3, is provided with isolation lid 9
Fitted completely with the inner wall section of memotron 3 at funnel-form Storage Cup 10, the infundibulate edge of the Storage Cup 10.It is arranged such, this
The blade and extract of utility model are preserved in pipe 1 and are provided with extract solution (the generally NaOH solution of 0.4M or so concentration), are stored
Isolation lid 9 is installed in pipe 3, funnel-form Storage Cup 10 is installed on isolation lid 9, the internal memory of funnel-form Storage Cup 10 is placed with buffer solution
(the generally Tris-cl solution of 0.1M or so concentration, pH8.0), by blade is put into blade and extract and preserves pipe 1 and manage when using
In body, the bottom of lapping device 2 insertion blade and extract with the first abrasive disk 4 are preserved in pipe 1 and fixed lid 6 is screwed
(ensureing blade between two abrasive disks), manipulates the handle 14 of the upper end termination of abrasive disk control-rod 5, by lapping device 2
Second abrasive disk 8 is pushed down on pushes down blade and left-right rotation is ground, and can up and down twitch control-rod in process of lapping makes blade
It is fully contacted with extract, memotron 3 and end-product memotron is directly sleeved on fixed lid 6 after the completion of grinding is screwed, now
The handle capable of being of abrasive disk control-rod 5 is contained in isolation lid 9, as shown in fig. 1；Then water-bath is carried out, by whole device after water-bath
Take out, remove memotron 3 and be inverted, pull out isolation lid 9 so that buffer solution is poured into memotron 3, and abrasive disk is pushed into leaf
Piece and extract preserve the bottom of pipe 1, leaf tissue is layered with supernatant, and appropriate supernatant is drawn with micropipettor, are directly added into
To in the ready end-product of lower memotron 3 and buffer storage pipe, that is, obtain leaf DNA.
Specific embodiment two：Present embodiment is illustrated with reference to Fig. 1, the DNA of plants rapid extraction of present embodiment is supporting
The lateral wall of blading and extract preservation pipe 1 and memotron 3 is provided with label and is placed with mouth 11.Be arranged such, in blade and
Extract preserves pipe 1 and the lateral wall label of memotron 3 is placed with mouth 11 mark for sticking same tag respectively so that various
When plant leaf blade DNA is extracted, the every kind of plant of separator of being more convenient for.
Specific embodiment three：Present embodiment is illustrated with reference to Fig. 1, the DNA of plants rapid extraction of present embodiment is supporting
The first abrasive disk 4 described in device is provided with some particulate abrasive projections 12.It is arranged such, particulate abrasive raised 12 is caused
Grinding operation it is in hgher efficiency, can more be fully ground plant leaf blade.
Specific embodiment four：Present embodiment is illustrated with reference to Fig. 1, the DNA of plants rapid extraction of present embodiment is supporting
The lower surface of the second abrasive disk 8 described in device is provided with some particulate abrasives raised 12 and some through holes 13.It is arranged such, the
Fully plant leaf blade is ground in the presence of two abrasive disks 8 and the first abrasive disk 4, while being arranged on the second abrasive disk 8
Some through holes 13 on body enable to process of lapping to pass through through hole 13 for extracting the extract solution of plant leaf blade DNA so that grind
Mill operation is smooth, it is to avoid the damage of related abrasive disk control-rod 5 in process of lapping, and abrasive disk control can be up and down twitched in process of lapping
Bar processed 5, makes blade be fully contacted with extract.
Present embodiment is the exemplary illustration to this patent, does not limit its protection domain, people in the art
Member can also be changed to its part, as long as no the Spirit Essence beyond this patent, all in the protection domain of this patent.
Claims (5)
1. DNA of plants rapid extraction corollary apparatus, including blade and extract preserve pipe (1), lapping device (2), memotron (3)
With the first abrasive disk (4), it is characterised in that：It is cylindrical tube, blade and extracting that described blade and extract preserve pipe (1)
The bottom that liquid preserves pipe (1) is provided with the first abrasive disk (4), and blade and extract to be preserved and be provided with grinding at the mouth of pipe of pipe (1)
Device (2), described lapping device (2) includes abrasive disk control-rod (5), fixed lid (6) and control-rod fixed mount (7), described
Fixed lid (6) be fixedly mounted on blade and extract is preserved at the mouth of pipe of pipe (1), fixed lid (6) upper end is provided with control-rod
Fixed mount (7), described abrasive disk control-rod (5) may be rotatably mounted on control-rod fixed mount (7), abrasive disk control-rod (5)
Blade being arranged on through fixed lid (6) and extract being preserved in pipe (1), the lower end end of abrasive disk control-rod (5) is provided with
The second abrasive disk (8) used cooperatively with the first abrasive disk (4), the upper end end of abrasive disk control-rod (5) is provided with handle
(14)；Described memotron (3) lid preserves the upper end of pipe (1) mounted in blade and extract.
2. DNA of plants rapid extraction corollary apparatus according to claim 1, it is characterised in that：In described memotron (3)
Be provided with isolation lid (9), isolation lid (9) on funnel-form Storage Cup (10) being installed, the infundibulate edge of the Storage Cup (10) with
The inner wall section of memotron (3) is fitted completely.
3. DNA of plants rapid extraction corollary apparatus according to claim 1, it is characterised in that：Blade and extract are preserved
The lateral wall of pipe (1) and memotron (3) is provided with label and is placed with mouth (11).
4. DNA of plants rapid extraction corollary apparatus according to claim 1, it is characterised in that：The first described abrasive disk
(4) some particulate abrasives projection (12) are provided with.
5. DNA of plants rapid extraction corollary apparatus according to claim 1, it is characterised in that：The second described abrasive disk
(8) lower surface is provided with some particulate abrasives projection (12) and some through holes (13).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621470683.8U CN206289239U (en) | 2016-12-28 | 2016-12-28 | DNA of plants rapid extraction corollary apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621470683.8U CN206289239U (en) | 2016-12-28 | 2016-12-28 | DNA of plants rapid extraction corollary apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206289239U true CN206289239U (en) | 2017-06-30 |
Family
ID=59098704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621470683.8U Withdrawn - After Issue CN206289239U (en) | 2016-12-28 | 2016-12-28 | DNA of plants rapid extraction corollary apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206289239U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434290A (en) * | 2016-12-28 | 2017-02-22 | 东北农业大学 | Plant DNA rapid extraction matching device |
| CN109576142A (en) * | 2018-12-05 | 2019-04-05 | 河南中医药大学 | A kind of plant material grinding device for DNA extraction operation |
| CN110564720A (en) * | 2019-09-04 | 2019-12-13 | 王中刚 | DNA extraction method |
| CN107523486B (en) * | 2017-09-14 | 2020-07-07 | 成都康辉生物科技有限公司 | Safe preservation equipment with high stability |
-
2016
- 2016-12-28 CN CN201621470683.8U patent/CN206289239U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434290A (en) * | 2016-12-28 | 2017-02-22 | 东北农业大学 | Plant DNA rapid extraction matching device |
| CN107523486B (en) * | 2017-09-14 | 2020-07-07 | 成都康辉生物科技有限公司 | Safe preservation equipment with high stability |
| CN109576142A (en) * | 2018-12-05 | 2019-04-05 | 河南中医药大学 | A kind of plant material grinding device for DNA extraction operation |
| CN110564720A (en) * | 2019-09-04 | 2019-12-13 | 王中刚 | DNA extraction method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20170630 Effective date of abandoning: 20181207 |