CN206804388U - A kind of ultralow temperature tissue grinder device - Google Patents
A kind of ultralow temperature tissue grinder device Download PDFInfo
- Publication number
- CN206804388U CN206804388U CN201720718787.4U CN201720718787U CN206804388U CN 206804388 U CN206804388 U CN 206804388U CN 201720718787 U CN201720718787 U CN 201720718787U CN 206804388 U CN206804388 U CN 206804388U
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- mortar
- temperature
- electric motor
- liquid nitrogen
- motor system
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Abstract
The utility model discloses a kind of ultralow temperature tissue grinder device, including low-temperature (low temperature) vessel, mortar, grinding pestle and electric motor system, the mortar are placed in the low-temperature (low temperature) vessel;The grinding pestle is rotatablely connected with the electric motor system, and the grinding pestle corresponds to mortar setting, and the electric motor system, which rotates, drives grinding pestle to carry out rotation grinding in mortar.The utility model is easy to use, solves the problems, such as well more difficult in the grinding of ultralow temperature the following group tissue samples.Sample bag in the environment of a relative closure, will not be made sample spluttering by mortar, grinding pestle and rubber ring;Grinding pestle contacts more abundant with mortar, and the rotating speed of electric motor system is controlled in suitable rotating speed, and this enables sample to be fully ground, and gained particle diameter is more homogeneous;Liquid nitrogen irrigating region separates with abrasive areas, and liquid nitrogen will not spill and hurt sb.'s feelings in process of lapping.
Description
Technical field
It the utility model is related to biological specimen processing technology field, more particularly to a kind of ultralow temperature tissue grinder device.
Background technology
In the research field such as biology, medicine, agronomy, herding, animal doctor, by specific biological components from biological specimen(Including
Plant tissue, animal tissue, cell, bacterium and yeast etc.)Middle separation and Extraction comes out, and is to apply one of universal routine techniques,
And the basis of follow-up study and experiment.In the extraction process of sample, it is necessary first to fully grind sample, make sample
This tissue or clasmatosis.The extraction of many samples need to be in ultralow temperature(With the temperature close of liquid nitrogen)Carried out just under environment
It can guarantee that its activity(Such as the activity of protein and RNA integrality), and conventional biological specimen is general under ultra-low temperature surroundings
In hard block structure.Therefore, grinding for biological specimen is become as essential first step operation.Tied with other bulks
Structure is compared, and many biological specimens have the characteristics that:1. small volume, the tissue samples particularly gathered from human body, therefore will
Ask grinding operation that there is high-recovery;2. most of biological specimen moistures are high, fragility is poor, is not easy to crush under normal temperature.Meanwhile
The grinding operation of biological specimen is carried out typically in Biochemical Lab, equipment therefor volume is not easy excessive.
At present, most domestic laboratory is when carrying out biological specimen grinding, using traditional Ginding process, Ji Jiangsheng
Thing sample is placed in mortar, is ground in liquid nitrogen with alms bowl pestle, is taken grinding gained sample powder to be transferred to EP pipes, afterwards, is added
Extracts reagent is extracted according to extraction flow.Many defects be present in this traditional method, when, for a small amount of or micro
Sample is not easy to operate;Second, sample is easily dropped to outside mortar during grinding, the loss of sample is caused;Third, gained
The powder particle size arrived is uneven, and grinding is not abundant enough;Fourth, the grinding effect of different personnel is different, the repeatability of experiment
Difference;Fifth, liquid nitrogen is easily spilt outside mortar, operating personnel are damaged.
Utility model content
In view of the shortcomings of the prior art, the utility model purpose be to provide one kind be easy to extract micro-example,
The ultralow temperature tissue grinder device of uniform, the practical safety of grinding.
To achieve these goals, the utility model uses following technical scheme:A kind of ultralow temperature tissue grinder device, bag
Low-temperature (low temperature) vessel, mortar, grinding pestle and electric motor system, the mortar is included to be placed in the low-temperature (low temperature) vessel;It is described grinding pestle with
The electric motor system rotation connection, the grinding pestle correspond to mortar setting, and the electric motor system, which rotates, drives grinding pestle to exist
Rotation grinding is carried out in mortar.
Preferably, one steel tank of setting in the low-temperature (low temperature) vessel, the steel tank are relative with the mortar provided with one
The lower groove answered, the steel tank are provided with the upper groove for being used for placing the electric motor system.
Preferably, the low-temperature (low temperature) vessel is liquid nitrogen tank, and the liquid nitrogen tank side is skewed, and steel is provided with the liquid nitrogen tank
Board slot, and the steel tank is tightly connected with liquid nitrogen tank.
Preferably, along being annular edge in the steel tank, the annular edge is erected at the liquid nitrogen tank top and sealing connects
Connect.
Preferably, rubber ring is equipped with the grinding pestle junction on the electric motor system, buffers electric motor system
To the pressure of steel tank, the heat transfer between electric motor system and steel tank is obstructed, prevents the sample in mortar from spilling.
Preferably, the liquid nitrogen tank is provided with insulation cover.
The utility model beneficial effect is:The utility model is easy to use, by by mortar, grinding pestle, steel tank, electronic
Machine system, liquid nitrogen tank and insulation cover cooperate to connect and set, and solve well in the grinding of ultralow temperature the following group tissue samples more
The problem of difficult.Set mortar and grinding pestle is relatively small, can handle the less sample of tissue sample relative quantity;Mortar,
Pestle and rubber ring are ground by sample bag in the environment of a relative closure, sample spluttering will not be made;Grinding pestle contacts with mortar
It is relatively abundant, and the rotating speed of electric motor system is controlled in suitable rotating speed, and this enables sample to be fully ground, gained
Grain diameter is more homogeneous;The rotating speed and milling time of electric motor system can be accurately controlled, substantially increases the repetition of experiment
Property;Liquid nitrogen irrigating region separates with abrasive areas, and liquid nitrogen will not spill and hurt sb.'s feelings in process of lapping.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
Or the required accompanying drawing used is briefly described in description of the prior art, it should be apparent that, drawings in the following description are only
It is some embodiments of the utility model, for those of ordinary skill in the art, is not paying the premise of creative work
Under, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is overall schematic of the present utility model;
Fig. 2 is a kind of structural representation of embodiment of the utility model;
Fig. 3 is the structure chart of mortar in the utility model;
Fig. 4 is the structure chart of the utility model light plate groove;
Fig. 5 is the structure chart that pestle and electric motor system are ground in the utility model;
Fig. 6 is the structure chart of liquid nitrogen tank in the utility model;
In figure:1 mortar, 2 grinding pestles, 3 steel tanks, 4 electric motor systems, 5 liquid nitrogen tanks, 6 insulation covers, 31 times grooves, groove on 32,
33 annular edges, 41 wires, 42 shift knobs, 43 rubber rings, 61 insulation cover handles.
Embodiment
The utility model is described further in conjunction with the accompanying drawings and embodiments.
A kind of ultralow temperature tissue grinder device, including low-temperature (low temperature) vessel, mortar, grinding pestle and electric motor system, the mortar
It is placed in the low-temperature (low temperature) vessel;The grinding pestle is rotatablely connected with the electric motor system, and the grinding pestle corresponds to mortar, institute
Stating electric motor system rotation drives grinding pestle to carry out rotation grinding in mortar.
Embodiment:
As shown in figures 1 to 6, a kind of ultralow temperature tissue grinder device, including low-temperature (low temperature) vessel, mortar 1, grinding pestle 2 and motor
System 4, in the present embodiment, the low-temperature (low temperature) vessel is liquid nitrogen tank 5, and electric motor system includes motor, decelerator, wire 41, switch
Button 42 and rubber ring 43;The liquid nitrogen tank 5 is provided with a steel tank 3 and insulation cover 6, and the steel tank 3 and liquid nitrogen tank 5 are tight
Close to be fitted and connected, the steel tank 3 includes upper groove 32, lower groove 31 and annular edge 33, and the annular edge 33 is erected at the liquid nitrogen
The upper edge of groove 5, places electric motor system 4 in the upper groove 32, and the lower groove 31 is used to placing mortar 1, in the present embodiment, grinds
Alms bowl 1 is that bottom is hemispherical, and main body is cylinder, interior empty construction and be ceramic material;Wherein, 4 turns of the electric motor system
Move and connect a grinding pestle 2, in the present embodiment, grinding pestle 2 is ceramic material, and the grinding pestle 2 and the mortar 1 in lower groove 31 are right
Should, grinding pestle 2 is stretched into the mortar 1, the sample in mortar 1 is ground under the drive of motor, the motor
Rubber ring 43 is provided between the lower section of system 4 and steel tank 3, pressure of the electric motor system to steel tank can be buffered, also barrier electricity
Heat transfer between motivation system 4 and steel tank 3, the sample being also prevented from mortar 1 are spilt.
In the present embodiment, the side of liquid nitrogen tank 5 is skewed, and is the cylindrical shape of upper big, lower small, and top is larger
Opening be easy to pouring into for liquid nitrogen.The liquid nitrogen tank 5 is made up of heat preserving and insulating material;The insulation cover 6 is by heat preserving and insulating material
It is made, the insulation cover 6 is provided with insulation cover handle 61.
The utility model is easy to use, by by mortar 1, grinding pestle 2, steel tank 3, electric motor system 4, the and of liquid nitrogen tank 5
Insulation cover 6 cooperates to connect and set, and solves the problems, such as well more difficult in the grinding of ultralow temperature the following group tissue samples.It is set
The mortar 1 and grinding pestle 2 put are relatively small, can handle the less sample of tissue sample relative quantity;Mortar 1, grinding pestle 2 and rubber
Sample bag in the environment of a relative closure, will not be made sample spluttering by circle 43;Grinding pestle 2 contacts relatively fully with mortar 1, and
And the rotating speed control of electric motor system 4, in suitable rotating speed, this enables sample to be fully ground, gained particle diameter compared with
To be homogeneous;The rotating speed and milling time of electric motor system 4 can be accurately controlled, substantially increases the repeatability of experiment;Liquid nitrogen
Irrigating region is separated with abrasive areas, and liquid nitrogen is within liquid nitrogen tank 5, steel tank 3 and insulation cover 6, in process of lapping
Liquid nitrogen will not spill and hurt sb.'s feelings.
In experimentation, most preferred embodiment can be completed by following steps:
1. liquid nitrogen tank 5 of the present utility model is placed on a stable table top or on ground, steel tank 3 is placed in
In liquid nitrogen tank 5, mortar 1 is placed in the lower groove 31 of steel tank 3.
2. adding a small amount of liquid nitrogen into liquid nitrogen tank 5, make whole system precooling, after system cooling, continuously add liquid nitrogen extremely
The lower edge of the upper groove 32 of steel tank 3.
3. the tissue sample of freezing is smashed to less than the internal diameter size of mortar 1, added as needed into mortar 1 appropriate
Tissue sample.
4. the electric motor system 4 with grinding pestle 2 is placed in the upper groove 32 of steel tank 3.
5. check whether the liquid nitrogen in liquid nitrogen tank 5 is sufficient, and insulation cover 6 is covered above liquid nitrogen tank 5.
6. connecting the power supply of electric motor system 4, suitable rotating speed and time are adjusted, shift knob 42 is pressed, sample is entered
Row grinding.
7. after end to be ground, the shift knob 42 of electric motor system 4 is pressed, deenergization, removes electric motor system 4,
It is rapid to take out mortar 1, ground sample is poured out, subsequent experimental is carried out to sample.
The standardized element that the utility model uses can commercially, shaped piece according to specification and accompanying drawing
Record can carry out customized, the specific connected mode of each part is using bolt ripe in the prior art, rivet, weldering
The conventional meanses such as connect, machinery, part and equipment are using in the prior art, conventional model, plus circuit connection using existing
Conventional connected mode, will not be described in detail herein in technology.
In this description, the utility model is described with reference to its specific embodiment.But it is clear that still can be with
Various modifications and alterations are made without departing from spirit and scope of the present utility model.Therefore, specification and drawings are considered as
It is illustrative and not restrictive.
Claims (6)
1. a kind of ultralow temperature tissue grinder device, it is characterised in that including low-temperature (low temperature) vessel, mortar(1), grinding pestle(2)With it is electronic
Machine system(4), the mortar(1)It is placed in the low-temperature (low temperature) vessel;The grinding pestle(2)With the electric motor system(4)Turn
Dynamic connection, the grinding pestle(2)Corresponding mortar(1)Set, the electric motor system(4)Rotate and drive grinding pestle(2)In mortar
(1)In carry out rotation grinding.
2. ultralow temperature tissue grinder device according to claim 1, it is characterised in that one is set in the low-temperature (low temperature) vessel
Steel tank(3), the steel tank(3)Provided with one and the mortar(1)Corresponding lower groove(31), the steel tank(3)If
Have for placing the electric motor system(4)Upper groove(32).
3. ultralow temperature tissue grinder device according to claim 1 or 2, it is characterised in that the low-temperature (low temperature) vessel is liquid nitrogen
Groove(5), the liquid nitrogen tank(5)Side is skewed, the liquid nitrogen tank(5)In be provided with steel tank(3), and the steel tank(3)
With liquid nitrogen tank(5)It is tightly connected.
4. ultralow temperature tissue grinder device according to claim 3, it is characterised in that the steel tank(3)Upper edge is ring
Shape side(33), the annular edge(33)It is erected at the liquid nitrogen tank(5)Top and sealed connection.
5. ultralow temperature tissue grinder device according to claim 3, it is characterised in that the electric motor system(4)It is upper with
The grinding pestle(2)Junction is provided with rubber ring, buffers electric motor system(4)To steel tank(3)Pressure, barrier motor system
System(4)With steel tank(3)Between heat transfer, prevent mortar(1)In sample spill.
6. ultralow temperature tissue grinder device according to claim 3, it is characterised in that the liquid nitrogen tank(5)Provided with insulation
Lid(6).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201720718787.4U CN206804388U (en) | 2017-06-20 | 2017-06-20 | A kind of ultralow temperature tissue grinder device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201720718787.4U CN206804388U (en) | 2017-06-20 | 2017-06-20 | A kind of ultralow temperature tissue grinder device |
Publications (1)
Publication Number | Publication Date |
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CN206804388U true CN206804388U (en) | 2017-12-26 |
Family
ID=60749205
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201720718787.4U Expired - Fee Related CN206804388U (en) | 2017-06-20 | 2017-06-20 | A kind of ultralow temperature tissue grinder device |
Country Status (1)
Country | Link |
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CN (1) | CN206804388U (en) |
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2017
- 2017-06-20 CN CN201720718787.4U patent/CN206804388U/en not_active Expired - Fee Related
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Legal Events
Date | Code | Title | Description |
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GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171226 Termination date: 20180620 |
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CF01 | Termination of patent right due to non-payment of annual fee |