CN204848892U - Ultrasonication biological cell examines extraction equipment - Google Patents
Ultrasonication biological cell examines extraction equipment Download PDFInfo
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- CN204848892U CN204848892U CN201520478433.8U CN201520478433U CN204848892U CN 204848892 U CN204848892 U CN 204848892U CN 201520478433 U CN201520478433 U CN 201520478433U CN 204848892 U CN204848892 U CN 204848892U
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- eddy flow
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Abstract
The utility model provides an ultrasonication biological cell examines extraction equipment, including the shell, characterized by: the bottom of shell is provided with driving motor mechanism, the upper portion of shell is provided with manual elevating system, driving motor mechanism connects the whirl cavity, the bottom of whirl cavity through terminal surface roller bearing set up in on the shell, be provided with a set of material collecting vat in the whirl cavity, the liquid pipe is connected to fall through deep groove ball bearing in the upper end of material collecting vat, liquid union coupling sample solution container falls, the sample solution container sets up on lift platform, the last ultrasonic wave cell disruption appearance that still is provided with of lift platform, lift platform connects manual elevating system, ultrasonic transducer is connected to ultrasonic wave cell disruption appearance, ultrasonic transducer passes the liquid pipe that falls stretches into in the whirl cavity. Complete equipment can effectually avoid because the condition that the separation and extraction that leads to of various organelle deposits can't go on together.
Description
Technical field
The utility model relates to Bioexperiment device field, specifically, relates to a kind of ultrasonication biomass cells core extraction equipment.
Background technology
The important process during extraction of biomass cells core in bio-science research, mainly by the method for physics or chemistry by cytoclasis, then by nucleus that chemical means extraction research needs.At present, common method of cell disruption mainly comprises high-pressure homogenization, high speed pearl mill method and supersonic method etc. is several.Ultrasonic disruption is adopted exactly electric energy to be converted to acoustic energy by transverter, this energy becomes small bubbles intensive one by one by liquid medium, these small bubbles burst rapidly, produce the energy as bomblet, thus play the effect of the materials such as smudge cells.At present, existing cell crushing instrument can only carry out simple pulverizing to cell, pulverize the cell solution produced also to need to use other chemistry or physical means to be separated, in the process of transhipment, the various organoids mixed through fragmentation in enchylema can be deposited in together, are unfavorable for nuclear extraction.
Summary of the invention:
The technical problems to be solved in the utility model is to provide a kind of ultrasonication biomass cells core extraction equipment, is convenient to the biomass cells core after extracting fragmentation.
The utility model adopts following technique means to realize goal of the invention:
A kind of ultrasonication biomass cells core extraction equipment, comprise shell, it is characterized in that: the bottom of described shell is provided with drive-motor mechanism, the top of described shell is provided with manual lifting mechanism, described drive-motor mechanism connects eddy flow cavity, the bottom of described eddy flow cavity is arranged on described shell by end face roller bearing, one group of collecting material groove is provided with in described eddy flow cavity, the upper end of described eddy flow cavity connects declining liquid tube by deep groove ball bearing, described declining liquid tube connects sample solution container, described sample solution container is arranged on lifting gantry, described lifting gantry is also provided with ultrasonic cell disruption instrument, described lifting gantry connects described manual lifting mechanism, described ultrasonic cell disruption instrument connects ultrasonic probe, described ultrasonic probe extend in described eddy flow cavity through described declining liquid tube.
As the further restriction to the technical program, described drive-motor mechanism comprises drive-motor, the output shaft of described drive-motor connects driving toothed gear, described driving toothed gear connects follower gear by synchronous cog belt, described follower gear is positioned on central rotating shaft, and described central rotating shaft is connected with described eddy flow cavity.
As the further restriction to the technical program, described manual lifting mechanism comprises the elevating bracket be connected with described lifting gantry, described elevating bracket center is threaded leading screw, described leading screw upper end connects handwheel, and the side of described shell is provided with the open slot coordinating described lifting gantry and elevating bracket to move up and down.
As the further restriction to the technical program, described shell is L-type.
As the further restriction to the technical program, described declining liquid tube is provided with end cap.
As the further restriction to the technical program, described declining liquid tube is provided with flow adjustment knob.
Compared with prior art, advantage of the present utility model and positively effect are: handwheel of the present utility model can control lifting gantry and elevating bracket is together elevated, when reaching the limit of position, the eddy flow cavity filling extraction liquid covers by end cap just, for follow-up centrifugation is ready.Such design ensure that compact equipment, and dropping liquid and ultrasonication simultaneously can be carried out simultaneously, can improve broken effect.The annulus of different diameter is processed with at eddy flow cavity bottom, as the collecting material groove of different sorts organoid, during work, first a certain amount of nucleus extraction solution to be added in eddy flow cavity, after drive-motor rotation starts, be followed by extraction liquid by ultrasonic disruption organoid out and do centrifugal rotation, because the speed of the sedimentation in certain centrifugal field of different organoid is different, the settling velocity of centre of sphere particle depends on the viscosity of its density, radius and suspension medium.Centrifugal certain hour in uniform suspension medium, the various organoid in tissue homogenate and other inclusion will rest on the different position of height because settling velocity is different.Increase centrifugal force and centrifugation time successively, these particles just can be made in batches to be deposited in the different collecting material groove of eddy flow cavity bottom by its size, weight, thus collect in batches.Organoid sedimentation order is successively nucleus, plastosome, lysosome and other microbodys, rrna and macromole.Whole plant dropping liquid, broken and centrifugal can at one time in complete, can effectively avoid because various organoid is deposited on the situation that the separation and Extraction that causes together cannot carry out.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
In figure, 1, shell, 2, eddy flow cavity, 3, end face roller bearing, 4, collecting material groove, 5, deep groove ball bearing, 6, declining liquid tube, 7, sample solution container, 8, lifting gantry, 9, ultrasonic cell disruption instrument, 10, ultrasonic probe, 11, drive-motor, 12, driving toothed gear, 13, synchronous cog belt, 14, follower gear, 15, central rotating shaft, 16, elevating bracket, 17, leading screw, 18, handwheel, 19, open slot, 20, end cap, 21, adjusting knob.
Embodiment:
Below in conjunction with embodiment, further illustrate the utility model.
See Fig. 1, the utility model comprises shell 1, the bottom of described shell 1 is provided with drive-motor mechanism, the top of described shell 1 is provided with manual lifting mechanism, described drive-motor mechanism connects eddy flow cavity 2, the bottom of described eddy flow cavity 2 is arranged on described shell 1 by end face roller bearing 3, one group of collecting material groove 4 is provided with in described eddy flow cavity 2, the upper end of described eddy flow cavity 2 connects declining liquid tube 6 by deep groove ball bearing 5, described declining liquid tube 6 connects sample solution container 7, described sample solution container 7 is arranged on lifting gantry 8, described lifting gantry 8 is also provided with ultrasonic cell disruption instrument 9, described lifting gantry 8 connects described manual lifting mechanism, described ultrasonic cell disruption instrument 9 connects ultrasonic probe 10, described ultrasonic probe 10 extend in described eddy flow cavity 2 through described declining liquid tube 6.
Described drive-motor mechanism comprises drive-motor 11, the output shaft of described drive-motor 11 connects driving toothed gear 12, described driving toothed gear 12 connects follower gear 14 by synchronous cog belt 13, and described follower gear 14 is positioned on central rotating shaft 15, and described central rotating shaft 15 is connected with described eddy flow cavity 2.
Described manual lifting mechanism comprises the elevating bracket 16 be connected with described lifting gantry 8, described elevating bracket 16 center is threaded leading screw 17, described leading screw 17 upper end connects handwheel 18, and the side of described shell 1 is provided with the open slot 19 coordinating described lifting gantry 8 and elevating bracket 16 to move up and down.
Described shell 1 is in L-type.
Described declining liquid tube 6 is provided with end cap 20.
Described declining liquid tube 6 is provided with flow adjustment knob 21.
When carrying out work, operator's rotation hand wheel 18 can control lifting gantry 8 and elevating bracket 16 is together elevated, and when reaching the limit of position, the eddy flow cavity 2 filling extraction liquid covers, for follow-up centrifugation is ready by end cap 20 just.Such design ensure that compact equipment, and dropping liquid and ultrasonication simultaneously can be carried out simultaneously, can improve broken effect.
The annulus of different diameter is processed with bottom eddy flow cavity 2, as the collecting material groove 4 of different sorts organoid, during work, first a certain amount of nucleus extraction solution to be added in eddy flow cavity 2, after drive-motor 11 rotation starts, be followed by extraction liquid by ultrasonic disruption organoid out and do centrifugal rotation, because the speed of the sedimentation in certain centrifugal field of different organoid is different, the settling velocity of centre of sphere particle depends on the viscosity of its density, radius and suspension medium.Centrifugal certain hour in uniform suspension medium, the various organoid in tissue homogenate and other inclusion will rest on the different position of height because settling velocity is different.Increase centrifugal force and centrifugation time successively, these particles just can be made in batches to be deposited in collecting material grooves 4 different bottom eddy flow cavity 2 by its size, weight, thus collect in batches.Organoid sedimentation order is successively nucleus, plastosome, lysosome and other microbodys, rrna and macromole.Whole plant dropping liquid, broken and centrifugal can at one time in complete, can effectively avoid because various organoid is deposited on the situation that the separation and Extraction that causes together cannot carry out.
Claims (6)
1. a ultrasonication biomass cells core extraction equipment, comprise shell, it is characterized in that: the bottom of described shell is provided with drive-motor mechanism, the top of described shell is provided with manual lifting mechanism, described drive-motor mechanism connects eddy flow cavity, the bottom of described eddy flow cavity is arranged on described shell by end face roller bearing, one group of collecting material groove is provided with in described eddy flow cavity, the upper end of described eddy flow cavity connects declining liquid tube by deep groove ball bearing, described declining liquid tube connects sample solution container, described sample solution container is arranged on lifting gantry, described lifting gantry is also provided with ultrasonic cell disruption instrument, described lifting gantry connects described manual lifting mechanism, described ultrasonic cell disruption instrument connects ultrasonic probe, described ultrasonic probe extend in described eddy flow cavity through described declining liquid tube.
2. ultrasonication biomass cells core extraction equipment according to claim 1, it is characterized in that: described drive-motor mechanism comprises drive-motor, the output shaft of described drive-motor connects driving toothed gear, described driving toothed gear connects follower gear by synchronous cog belt, described follower gear is positioned on central rotating shaft, and described central rotating shaft is connected with described eddy flow cavity.
3. ultrasonication biomass cells core extraction equipment according to claim 1, it is characterized in that: described manual lifting mechanism comprises the elevating bracket be connected with described lifting gantry, described elevating bracket center is threaded leading screw, described leading screw upper end connects handwheel, and the side of described shell is provided with the open slot coordinating described lifting gantry and elevating bracket to move up and down.
4. ultrasonication biomass cells core extraction equipment according to claim 1, is characterized in that: described shell is L-type.
5. ultrasonication biomass cells core extraction equipment according to claim 1, is characterized in that: described declining liquid tube is provided with end cap.
6. ultrasonication biomass cells core extraction equipment according to claim 1, is characterized in that: described declining liquid tube is provided with flow adjustment knob.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520478433.8U CN204848892U (en) | 2015-07-06 | 2015-07-06 | Ultrasonication biological cell examines extraction equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520478433.8U CN204848892U (en) | 2015-07-06 | 2015-07-06 | Ultrasonication biological cell examines extraction equipment |
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| Publication Number | Publication Date |
|---|---|
| CN204848892U true CN204848892U (en) | 2015-12-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520478433.8U Expired - Fee Related CN204848892U (en) | 2015-07-06 | 2015-07-06 | Ultrasonication biological cell examines extraction equipment |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108245052A (en) * | 2018-03-27 | 2018-07-06 | 李台 | A kind of ultrasonic extracting machine |
-
2015
- 2015-07-06 CN CN201520478433.8U patent/CN204848892U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108245052A (en) * | 2018-03-27 | 2018-07-06 | 李台 | A kind of ultrasonic extracting machine |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151209 Termination date: 20160706 |