CN206378348U - A kind of organization embedding device for being used to prepare frozen tissue section - Google Patents

A kind of organization embedding device for being used to prepare frozen tissue section Download PDF

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Publication number
CN206378348U
CN206378348U CN201621301116.XU CN201621301116U CN206378348U CN 206378348 U CN206378348 U CN 206378348U CN 201621301116 U CN201621301116 U CN 201621301116U CN 206378348 U CN206378348 U CN 206378348U
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CN
China
Prior art keywords
embedding device
tissue section
frozen tissue
organization embedding
cylinder body
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Expired - Fee Related
Application number
CN201621301116.XU
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Chinese (zh)
Inventor
马薇
李力燕
郭建辉
杨金伟
李兴统
王先斌
王通通
代云飞
张泰�
李永进
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Kunming Medical University
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Kunming Medical University
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Priority to CN201621301116.XU priority Critical patent/CN206378348U/en
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Publication of CN206378348U publication Critical patent/CN206378348U/en
Expired - Fee Related legal-status Critical Current
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Abstract

The utility model discloses a kind of organization embedding device for being used to prepare frozen tissue section, the organization embedding device includes embedded box, the embedded box includes being provided with the round platform (4) supporting with cylinder body at the top of the push rod (2) that bottom margin is provided with the cylinder body (1) of bulge-structure (3) and is arranged in cylinder body (1), the push rod (2).The embedding device that the utility model is provided can embed different size of tissue samples.Tissue samples after embedding, regular shape, surface is smooth, and tissue is placed in the direction that can be cut into slices as required, it also avoid the waste of unnecessary embedding medium, be particularly suitable for use in freezing microtome section.

Description

A kind of organization embedding device for being used to prepare frozen tissue section
Technical field
The utility model belongs to organization embedding technical field, more particularly to a kind of tissue for being used to prepare frozen tissue section Embed device.
Background technology
Embedded box is to provide the process of performance support or chemoproection for embedded material powder or other block structures. The processing quality to microorganism or cell tissue can be strengthened, it is to avoid stripping and slicing is repaired.Investment is usually used in microorganism, animal and In the immobilization material of plant cell.Unlike material and the embedded box of shape embed into material different shapes.
It is that directly tissue is placed in special tissue firm banking (sample carrier) that frozen section is done in common laboratory, See Fig. 1, embedding medium embedding is then added dropwise, then cut into slices after freezing.It is difficult to the bag to form regular shape during use Bury block, it is impossible to control the depth of tissue placement and the direction of histotomy, be unfavorable for embedding microtissue, and easily cause The spilling of embedding medium and waste.
The Chinese patent application of Application No. 201320248268.8 discloses a kind of FFPE box, include box body and Lid, the top of the box body and a side are opening, and slot, the opening and the opening of the side of the slot are provided with base Direction is consistent;The lid is L-type plate, and one lateral sizes match with slot.The embedded box is applied to carry out the paraffin of tissue Embedding, makes paraffin tissue sections after embedding, and the utility model provides a kind of tissue bag for being used to prepare frozen tissue section Device is buried, using carrying out frozen tissue section after embedding medium OCT investing tissues.The embedded box box body is larger, is unfavorable for embedding micro- Cell (being, for example, less than 5mm tissue), the area cut into slices when being cut into slices after being embedded for microtissue is excessive, is difficult to find group Knit, and cause the waste of embedding medium.The embedded box carry out microtissue embedding when uncontrollable histotomy direction with And be difficult to take out after organizing the depth of placement, the embedded box investing tissue.
Utility model content
In view of the shortcomings of the prior art, the utility model provides a kind of organization embedding dress for being used to prepare frozen tissue section Put, the embedding apparatus structure is simple, the microtissue for the progress frozen tissue section that is particularly suitable for use in (is, for example, less than 5mm group Knit) embedding, tissue can be controlled laterally or longitudinally to embed, will can organize need direction to be placed in the bottom of embedded box by section Portion, the depth that tissue can be controlled to place, the waste of embedding medium is not easily caused, can be taken out easily after embedding.
The technical solution of the utility model is as follows:A kind of organization embedding device for being used to prepare frozen tissue section, it is described Organization embedding device includes embedded box, and the embedded box includes cylinder body 1 and setting of the bottom margin provided with bulge-structure 3 Push rod 2 in cylinder body 1, the top of push rod 2 is provided with round platform 4, and the diameter of round platform and the internal diameter of cylinder body are adapted.
Further, the bottom of push rod 2 is provided with base 5, is easy to vertical placement.
Further, the round platform 4 is cylindrical rubber pad, is conducive to promoting above and below push rod.
Further, the top of cylinder body 1 is provided with cover 6, it is to avoid dust etc. is dropped into embedded box.
Further, the cover 6 is connected on bulge-structure 3 by fixing band, it is to avoid cover drop loss etc..
Further, the embedded box is made of resin material, a diameter of 1~5cm of the cylinder body.
The organization embedding device also includes support 7, and the top of support 7 is provided between multiple necks 8, adjacent neck and stayed There is space, be easy to place multiple embedded boxes.
Operation principle of the present utility model is as follows:Embedded box of the present utility model includes setting in cylinder body, cylinder body Have and round platform is provided with the top of push rod, push rod, during investing tissue, push rod moves the lowermost end of cylinder body to, tissue is needed by section Direction is placed on the push rod round platform of embedded box bottom, and embedding medium OCT (optimal cutting temperature are added dropwise Compound) into embedded box, make marks or label on embedded box paper, covers after cover embedded box being put into -80 DEG C of ice Case preserves slice, after refrigeration, promotes push rod to move upwards, releases the tissue after embedding, to enable embedded box stably Stand on the table, push rod bottom is provided with base, base area is more than cylinder floor space.Distinguish sometimes for multiple tissues are carried out Embedded, multiple embedded boxes can be placed on same support, frame upper is provided between row's neck, adjacent neck and left Space, is easy to put multiple embedded boxes, because the bottom margin of cylinder body is provided with bulge-structure, when embedded box is placed in neck, Push rod is stuck in neck, and bulge-structure is located at the top of neck.
Compared with prior art, the utility model has the advantages that:The embedding device that the utility model is provided can Embed different size of tissue samples.Tissue samples after embedding, regular shape, surface is smooth, can cut into slices as required Direction place tissue, it also avoid the waste of unnecessary embedding medium.It can be taken out easily after embedding.Used in the organization embedding device Material can be through (such as subzero 20 DEG C~subzero 80 DEG C) freezing of low temperature and ultralow temperature.
Brief description of the drawings
Fig. 1 is the structural representation of firm banking (sample carrier);
Fig. 2 is the embedding apparatus structure schematic diagram that embodiment 1 is provided;
Fig. 3 is the structural representation of support;
Fig. 4 is the embedding apparatus structure schematic diagram that embodiment 2 is provided;
Marked in figure:1- cylinder bodies, 2- push rods, 3- bulge-structures, 4- round platforms, 5- bases, 6- covers, 7- supports, 8- Neck.
Embodiment
The technical solution of the utility model is described in further details with specific embodiment below in conjunction with the accompanying drawings.Embodiment 1
As shown in Fig. 2 the organization embedding device includes embedded box, the embedded box includes bottom margin and tied provided with raised The cylinder body 1 of structure 3 and the push rod 2 being arranged in cylinder body 1, the top of push rod 2 are provided with supporting with cylinder body Round platform 4.
The top of cylinder body 1 is provided with cover 6, and the bottom of push rod 2 is provided with base 5, and round platform 4 is cylindrical rubber pad, is conducive to Promoted above and below push rod.Cover 6 is connected on bulge-structure 3 by fixing band, it is to avoid cover drops loss.
Embedded box is made of resin material, a diameter of 1~2cm of the cylinder body.
The use process of the embedding device is as follows:Embedded box is stood on the table, and during investing tissue, push rod moves cylinder to The lowermost end of body, needs direction (laterally or longitudinally) to be placed on the round platform of embedded box bottom in tissue, bag is added dropwise by section Agent OCT (optimal cutting temperature compound) is buried into embedded box, makes marks or pastes on embedded box Label paper, covers and embedded box is put into -80 DEG C of refrigerators after cover preserves slices, after refrigeration, promote push rod to move upwards, make bag Tissue after burying is released, and is cut into slices in the embedding fixed seat being placed in Fig. 1.
Embodiment 2
As shown in Figure 3 and Figure 4, the organization embedding device also includes support 7, and the upper level of support 7 is provided with multiple Space is left between neck 8, adjacent neck, is easy to place multiple embedded boxes.The bottom margin of cylinder body is provided with bulge-structure, When embedded box is placed in neck, push rod is stuck in neck, and bulge-structure is located at the top of neck.

Claims (7)

1. a kind of organization embedding device for being used to prepare frozen tissue section, it is characterised in that the organization embedding device includes Embedded box, the embedded box includes bottom margin and is provided with the cylinder body (1) of bulge-structure (3) and is arranged on cylinder body (1) round platform (4) is provided with the top of the push rod (2) in, the push rod (2).
2. the organization embedding device as claimed in claim 1 for being used to prepare frozen tissue section, it is characterised in that the push rod (2) bottom is provided with base (5).
3. the organization embedding device as claimed in claim 1 for being used to prepare frozen tissue section, it is characterised in that the round platform (4) it is cylindrical rubber pad.
4. the organization embedding device as claimed in claim 1 for being used to prepare frozen tissue section, it is characterised in that the cylinder Cover (6) is provided with the top of body (1).
5. the organization embedding device as claimed in claim 4 for being used to prepare frozen tissue section, it is characterised in that the cover (6) it is connected to by fixing band on bulge-structure (3).
6. being used for as described in Claims 1 to 5 is any prepares the organization embedding device of frozen tissue section, it is characterised in that The organization embedding device also includes support (7), and support (7) top is provided between multiple necks (8), adjacent neck and left Space, is easy to place multiple embedded boxes.
7. the organization embedding device as claimed in claim 6 for being used to prepare frozen tissue section, it is characterised in that the embedding Box is made of resin material, a diameter of 1~5cm of the cylinder body.
CN201621301116.XU 2016-11-30 2016-11-30 A kind of organization embedding device for being used to prepare frozen tissue section Expired - Fee Related CN206378348U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201621301116.XU CN206378348U (en) 2016-11-30 2016-11-30 A kind of organization embedding device for being used to prepare frozen tissue section

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201621301116.XU CN206378348U (en) 2016-11-30 2016-11-30 A kind of organization embedding device for being used to prepare frozen tissue section

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107478492A (en) * 2017-10-13 2017-12-15 上海海洋大学 One group is used for the device that frozen section tissue embeds in advance
CN109682667A (en) * 2018-12-17 2019-04-26 无锡启盛实验设备有限公司 A kind of frost embedding machine
CN110398408A (en) * 2019-08-14 2019-11-01 中国科学院深圳先进技术研究院 Imbedded mold and its application method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107478492A (en) * 2017-10-13 2017-12-15 上海海洋大学 One group is used for the device that frozen section tissue embeds in advance
CN109682667A (en) * 2018-12-17 2019-04-26 无锡启盛实验设备有限公司 A kind of frost embedding machine
CN110398408A (en) * 2019-08-14 2019-11-01 中国科学院深圳先进技术研究院 Imbedded mold and its application method

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GR01 Patent grant
GR01 Patent grant
CB03 Change of inventor or designer information
CB03 Change of inventor or designer information

Inventor after: Ma Wei

Inventor after: Li Yongjin

Inventor after: Li Liyan

Inventor after: Guo Jianhui

Inventor after: Yang Jinwei

Inventor after: Li Xingtong

Inventor after: Wang Xianbin

Inventor after: Wang Tongtong

Inventor after: Dai Yunfei

Inventor after: Zhang Tai

Inventor before: Ma Wei

Inventor before: Li Yongjin

Inventor before: Li Liyan

Inventor before: Guo Jianhui

Inventor before: Yang Jinwei

Inventor before: Li Xingtong

Inventor before: Wang Xianbin

Inventor before: Wang Tongtong

Inventor before: Dai Yunfei

Inventor before: Zhang Tai

CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170804

Termination date: 20191130