CN203723325U - Novel trepanning type micro-scale cryopreservation tube - Google Patents
Novel trepanning type micro-scale cryopreservation tube Download PDFInfo
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- CN203723325U CN203723325U CN201420110456.9U CN201420110456U CN203723325U CN 203723325 U CN203723325 U CN 203723325U CN 201420110456 U CN201420110456 U CN 201420110456U CN 203723325 U CN203723325 U CN 203723325U
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- tube
- cryopreservation tube
- cryopreservation
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Abstract
The utility model discloses a novel trepanning type micro-scale cryopreservation tube which comprises a tube body and a tube cover. The tube is characterized in that an embedded type tube opening is formed in the top of the tube body; the tube cover is mounted on the embedded type tube opening; a tube bottom embedding opening is formed in the bottom of the tube body; one or more planar tube bottoms are arranged inside the tube body. After the trepanning type micro-scale cryopreservation tube designed by the utility model is fed into liquid nitrogen, liquid nitrogen flows into the cryopreservation tube from small holes and is in direct contact with a tissue sample, then the tissue can be frozen quickly, the activity of enzymes of the tissue sample is effectively inhibited, and thus the sample is effectively preserved; moreover the size of the cryopreservation tube is reduced, and each cryopreservation tube can only preserve a small amount of samples for meeting the requirements of once or twice of experiment, so that the samples are prevented from being repeated taken, frozen and molten. To overcome the problem that only a small amount of the samples can be preserved because of the small capacity of the cryopreservation tube, the cryopreservation tube is designed into an embedded type, a plurality of cryopreservation tubes can be used after being embedded together, and thus one same sample can be conveniently preserved.
Description
Technical field
The utility model relates to a kind of novel perforating trace cryopreservation tube, belongs to biological specimen save set field.
Background technology
In the genome times afterwards comprehensively, the functional study of genomic expression regulation and protein becomes the main body of molecular biology research.And the research of transcriptional level and translation skill all be unable to do without and extract RNA or protein from test sample.Because the activity of RNA enzyme and protease is stronger, Sample preservation is improper is easy to cause the degraded of RNA and protein in sample, once degrade, the especially degraded of RNA, will bring difficulty or cause its failure to follow-up test.How protecting sample to avoid the degraded of RNA and protein is the key factor that concerns follow-up test success or failure, and the rare samples such as a lot of pathology and large animal are because sampling difficulty greatly more needs careful preservation.Therefore, from what sample sample, take and preserve the activity that all will strictly control RNA enzyme and protease.
Liquid nitrogen can reach the extremely low temperature of-196 ℃, can effectively suppress the activity of RNA enzyme and protease, thereby can preserve test sample by longer-term high-quality.When sampling; for protective tissue sample and facilitate follow-up test to take; after collection, need will to organize sample to put into cryopreservation tube rapidly, then cryopreservation tube is dropped in liquid nitrogen, if organize sample can directly contact liquid nitrogen quick-frozen immediately, to suppressing the activity of various enzymes, have vital effect.Now, the cryopreservation tube generally using is of a size of high 4.5cm(from cryopreservation tube caping end to managing the end), pipe shaft diameter 1cm, pipe covers diameter 1.3cm, and cryopreservation tube tube wall is closed design, organizes sample cannot directly immerse liquid nitrogen in this process.Because when sampling is for collecting sample as much as possible, generally all cryopreservation tube is filled after organizing sample and preserved; If each cryopreservation tube deposits a small amount of sample in, cause again cryopreservation tube to use and too much cause the waste of liquid nitrogen container space.And in follow-up test, each test often only needs a small amount of tissue samples, during due to sampling, cryopreservation tube is filled sample, by liquid nitrogen flash freezer posterior synechia.Therefore, after all cryopreservation tube external force will being smashed, again the sample of organizing of adhesion is smashed at every turn, after taking, remaining tissue block is proceeded to the follow-up continuation of insurance of new cryopreservation tube and deposit.In this process, because operating time is at normal temperatures long, be easy to cause organizing RNA or the protein degradation in sample, sample is caused to irremediable loss, and in fragmentation process, easily cause that tissue block is cracked to splash, cause to organize sample to pollute or be difficult to and reclaim.Therefore, invent a kind of cryopreservation tube of the rapid infiltration of liquid nitrogen and low capacity that can make is necessary very much.
Utility model content
In order to solve the problems of the technologies described above, the utility model provides a kind of novel perforating trace cryopreservation tube.
The purpose of this utility model is carried out specific implementation by the following technical programs:
A novel perforating trace cryopreservation tube, comprises body and Guan Gai, and the top of described body has the embedded mouth of pipe, and described pipe lid is arranged on the embedded mouth of pipe, and the bottom of body has the embedding mouthful of the pipe end, at the bottom of the inside of body is provided with at least one flat tube.
Further, on the sidewall circumference of described body, have the perforate of many groups, organize between perforate more and mutually separate, to maintain a certain distance.
Described body and pipe lid adopt low-temperature resistant plastic.
Described body is transparent configuration.
Described body is cylindric.
Rounded at the bottom of described flat tube.
The perforating low capacity cryopreservation tube of the utility model design, after cryopreservation tube is put in liquid nitrogen, liquid nitrogen is flowed in cryopreservation tube and is directly contacted with group sample by aperture, and snap frozen tissue effectively suppresses to organize the activity of various enzymes in sample, thereby effectively preserves sample; And by cryopreservation tube size reduction, each cryopreservation tube is only put into a small amount of sample, meet once or twice experiment demand, avoid sample repeatedly to take freeze thawing.In order to overcome the little less problem of sample that deposits in causing of cryopreservation tube capacity, cryopreservation tube is designed to nested type, the use that can be superimposed together of several cryopreservation tubes, facilitates the preservation of same sample.
Accompanying drawing explanation
According to drawings and embodiments the utility model is described in further detail below.
Fig. 1 is the structure chart of a kind of novel perforating trace cryopreservation tube described in the utility model embodiment.
Fig. 2 is two perforating trace cryopreservation tube overlaying structure figure.
1, Guan Gai, is of a size of high 1cm, external diameter 1.1cm, internal diameter 1cm; 2, the embedded mouth of pipe, diameter is 0.99cm; 3, body, internal diameter is 1cm, thickness is 0.5mm, is highly 1cm; 4, at the bottom of flat tube; 5, the pipe end, embeds mouth, and height is 1cm; 6, perforate, diameter is 1mm.
Embodiment
As shown in Figure 1-2, a kind of novel perforating trace cryopreservation tube described in the utility model embodiment, comprise that body 3 and pipe cover 1, the top of described body 3 has the embedded mouth of pipe 2, described pipe lid 1 is arranged on the embedded mouth of pipe 2, the bottom of body 3 has a pipe end and embeds mouthfuls 5, and the inside of body 3 is provided with at the bottom of at least one flat tube 4.
Further, on the sidewall circumference of described body 3, have the perforate 6 of many groups, organize between perforate 6 more and mutually separate, to maintain a certain distance.
Described body 3 and pipe cover 1 and adopt low-temperature resistant plastic.
Described body 3 is transparent configuration.
Described body 3 is cylindric.
At the bottom of described flat tube, 4 is rounded.
When sampling, will organize sample to be cut into small pieces, put into rapidly cryopreservation tube, the embedded mouth of pipe 2 is embedded to pipe and cover in 1, sealing cryopreservation tube; For the ease of same tissue, preserve, can be by the same tissue of a plurality of cryopreservation tube collections, the embedded mouth of pipe 2 of each cryopreservation tube is embedded into successively and embeds mouthfuls 5 at the bottom of the pipe of another cryopreservation tube, can form the cryopreservation tube of stack.Each cryopreservation tube is all equipped with lid, if use and cover upper tube cap separately.After sampling, immediately cryopreservation tube is dropped in liquid nitrogen container, liquid nitrogen is deep in cryopreservation tube and is directly contacted with organizing sample the object that reaches quick-frozen by pipe shaft perforate 6.During each test; as required one or more cryopreservation tubes are departed from from the large cryopreservation tube embedding; both can avoid the object of traditional broken cryopreservation tube chorista sample; can protect again the temporary transient no sample of organizing to avoid degraded and physical damage, can greatly accelerate the time of separating sample and slow down histioid degraded.
The above; it is only preferred embodiment of the present utility model; not in order to limit the utility model, any trickle modification that every foundation technical spirit of the present utility model is done above embodiment, be equal to and replace and improve, within all should being included in the protection domain of technical solutions of the utility model.
Claims (6)
1. a novel perforating trace cryopreservation tube, comprises body and Guan Gai, it is characterized in that, the top of described body has the embedded mouth of pipe, described pipe lid is arranged on the embedded mouth of pipe, and the bottom of body has the embedding mouthful of the pipe end, at the bottom of the inside of body is provided with at least one flat tube.
2. a kind of novel perforating trace cryopreservation tube as claimed in claim 1, is characterized in that, has the perforate of many groups on the sidewall circumference of described body, organizes between perforate more and mutually separates, to maintain a certain distance.
3. a kind of novel perforating trace cryopreservation tube as claimed in claim 1, is characterized in that, described body and pipe lid adopt low-temperature resistant plastic.
4. a kind of novel perforating trace cryopreservation tube as claimed in claim 1, is characterized in that, described body is transparent configuration.
5. a kind of novel perforating trace cryopreservation tube as claimed in claim 1, is characterized in that, described body is cylindric.
6. a kind of novel perforating trace cryopreservation tube as claimed in claim 5, is characterized in that, rounded at the bottom of described flat tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420110456.9U CN203723325U (en) | 2014-03-12 | 2014-03-12 | Novel trepanning type micro-scale cryopreservation tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420110456.9U CN203723325U (en) | 2014-03-12 | 2014-03-12 | Novel trepanning type micro-scale cryopreservation tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203723325U true CN203723325U (en) | 2014-07-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420110456.9U Expired - Fee Related CN203723325U (en) | 2014-03-12 | 2014-03-12 | Novel trepanning type micro-scale cryopreservation tube |
Country Status (1)
| Country | Link |
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| CN (1) | CN203723325U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105890965A (en) * | 2014-10-09 | 2016-08-24 | 江南大学 | Fenestrated freezing tube for storing biological tissue |
| WO2023288181A1 (en) * | 2021-07-13 | 2023-01-19 | Sana Biotechnology, Inc. | Cryogenic storage racks and associated systems, devices, and methods |
-
2014
- 2014-03-12 CN CN201420110456.9U patent/CN203723325U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105890965A (en) * | 2014-10-09 | 2016-08-24 | 江南大学 | Fenestrated freezing tube for storing biological tissue |
| WO2023288181A1 (en) * | 2021-07-13 | 2023-01-19 | Sana Biotechnology, Inc. | Cryogenic storage racks and associated systems, devices, and methods |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140723 Termination date: 20150312 |
|
| EXPY | Termination of patent right or utility model |