CN101451928A - Multifunction organization embedding and refrigerating plant - Google Patents
Multifunction organization embedding and refrigerating plant Download PDFInfo
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- CN101451928A CN101451928A CNA2007101956313A CN200710195631A CN101451928A CN 101451928 A CN101451928 A CN 101451928A CN A2007101956313 A CNA2007101956313 A CN A2007101956313A CN 200710195631 A CN200710195631 A CN 200710195631A CN 101451928 A CN101451928 A CN 101451928A
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- liquid nitrogen
- tissue
- thermos
- adjustable suspension
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Abstract
This invention is a tissue embed-freeze apparatus for frozen tissue section used for life science experiment and medical pathological tissue examination, comprised of a multifunctional liquid nitrogen storage thermostatic bottle and an adjustable suspending water tank. The multifunctional liquid nitrogen storage thermostatic bottle is internally designed with a conical baffle for the adjustable suspending water tank to enter, used for freezing the embedded tissue and reducing volatilization of liquid nitrogen therein. The adjustable suspending water tank is disposed with two freely operable pull rods on two sides thereof, in order to adjust immerging depth and position of the water tank into the liquid nitrogen in the thermostatic bottle within 0 to 30cm according to actual need. With utilization, this invention can rapidly and conveniently carry out high quality freezing process can be carried out on embedded tissue and embryo as a special tissue embed-freeze apparatus, especially suitable for life science, pathology and anatomy, etc., which simultaneously bring new improvement for existing tissue embed-freeze module.
Description
Technical field
The present invention is a kind of utensil that is used for the check of Life Science Experiment and medical Histopathology, specifically in carrying out the freezing microtome section experimentation, and a kind of device that is used for investing tissue and embryo liquid chilled nitrogen.
Background technology
The making of freezing microtome section is the important content of immunohistochemistry technology and in situ hybridization tissue chemical technology, and accurately locate by endoantigen (perhaps functional gene) to organizing for the specific antibody (the perhaps nucleic acid probe of special marking) by utilizing mark, qualitative, quantitative and definite different development stage tissue specific expression detects.Organization embedding and refrigerating plant is to carry out organizing the indispensable conventional apparatus of freezing microtome section in vital tissues and the pathological research, also is an important step of producing in tissue and the embryo's slicing processes.Because the organization embedding and refrigerating plant of neither one specialization has increased the freezing difficulty that places the embedded box sample greatly, it is very important for the quality of freezing microtome section therefore to design a kind of specialized organization embedding and refrigerating plant.
Traditional organization embedding refrigerating process: carry out freezing microtome section tissue and embryo's size as required, preparation is the embedded box of size suitably, sample is put in add OCT embedding compound in the tinfoil paper carton, places the container of 30% isopropyl alcohol, container is suspended in the liquid nitrogen, thereby carries out freezing processing.But owing to there is not the organization embedding and refrigerating plant of specialization at present, need a portable container that fills isopropyl alcohol, immerse in the isopropyl alcohol with tweezers gripping embedded box on the other hand, increased the process of manual operation, fill the liquid nitrogen bottle of liquid nitrogen because opening is bigger, the liquid nitrogen volatilization too much need be added liquid nitrogen in the operating process again, causes the liquid nitrogen waste on the other hand again.Traditional thus organization embedding process is loaded down with trivial details, wastes time and energy, and influences work efficiency, has very much improved necessity.
Summary of the invention
The objective of the invention is to remedy defective and the deficiency that prior art exists, and a kind of multifunction organization embedding and refrigerating plant that is used for the specialization of Life Science Experiment and the check of medical Histopathology that provides.
For achieving the above object, the present invention takes following technical scheme:
(1) indoor design of liquid nitrogen thermos the conical plate washer that can allow adjustable suspension waters case to enter just, it is freezing both can to have carried out investing tissue, can reduce the volatilization of bottle interior liquid nitrogen again.
(2) height of waters case passes through to regulate in the liquid nitrogen thermos.
(3) design of the top of liquid nitrogen thermos has " U " type handle, can be convenient for carrying on the one hand, is fit to outstanding the connecing of hook on case two lateral bracing struts of adjustable suspension waters on the other hand.
Advantage of the present invention and benefit: the conical plate washer of liquid nitrogen thermos inside, both can guarantee the freezing of investing tissue, can reduce the volatilization of bottle interior liquid nitrogen again, can effectively prevent and treat the defective of getting repeatedly because of the liquid nitrogen deficiency; The pull bar that is positioned at case both sides, adjustable suspension waters is regulated, and can regulate the height of waters case in liquid nitrogen easily and carry out the freezing of embedding sample; The top design of liquid nitrogen thermos has " U " type handle, is fit to outstanding the connecing of hook on case two lateral bracing struts of adjustable suspension waters, has deducted the trouble that in the past needs staff water lift territory case to cause.The present invention has reduced the manual operation process, has saved research funding, has improved work efficiency effectively.
The present invention will be further described below in conjunction with accompanying drawing and preferred forms, so that the public has whole to summary of the invention and understand fully, and is not qualification to protection domain of the present invention.Aforementioned part fully discloses the protection domain that the present invention can implement, and therefore allly any well known in the artly is equal to replacement according to what the disclosure of invention was carried out, all belongs to infringement of the present invention.
Description of drawings
Below in conjunction with drawings and Examples the present invention is further specified:
Fig. 1 is an one-piece construction synoptic diagram of the present invention.
Fig. 2 stores the thermos synoptic diagram for the multi-functional liquid nitrogen of the present invention.
Fig. 3 is the adjustable suspension of a present invention waters case synoptic diagram.
Fig. 4 is case both sides, the adjustable suspension of a present invention waters telescopic lever synoptic diagram.
Embodiment
See also shown in the accompanying drawing 1～4, in use, the embedded box of tissue and embryo's sample being put into suitable size adds OCT embedding compound, places the adjustable suspension waters case of 30% isopropyl alcohol.According to the experiment needs, utilize pull bar that both sides can free push-and-pull to regulate the waters case and immerse the degree of depth and position in the thermos liquid nitrogen, carry out the freezing of tissue sample.
Embodiment
The present invention has liquid nitrogen and stores thermos (1) and have the handle (2) that can be convenient for carrying, and inside has one and can allow conical plate washer (3) that adjustable suspension waters case (4) enters to reduce the volatilization of liquid nitrogen just.Adjustable suspension waters case (4) utilizes the hook (6) on two lateral bracing struts (5) top to be suspended from liquid nitrogen and stores in the thermos (1).And can regulate height and the position that is suspended from thermos waters case by the pull bar (5) that adjusting is arranged in the retractable of adjustable suspension waters case (4) both sides, can conveniently carry out the freezing of embedding sample, be convenient to operation, reduced complex operation in the past, not easy-operating shortcoming.
Liquid nitrogen stores thermos and is designed to: high 30cm, the cylindrical structural of end diameter 20cm, the design of 15cm place, top has the handle that can be convenient for carrying, has conical plate washer that the diameter that can allow adjustable suspension waters case to enter just is 10cm at distance insulation bottleneck 10cm place, both can satisfy the freezing of investing tissue, can reduce the volatilization of liquid nitrogen again, save research funding.Adjustable suspension waters case is designed to: high 15cm, the cylindrical structural of end diameter 10cm utilizes the hook on two lateral bracing strut tops to be suspended from liquid nitrogen storage thermos handle.And can be by regulating pull bar at the retractable of case both sides, adjustable suspension waters between 0～30cm, regulate the height and the position that are suspended from waters case in the thermos, can conveniently carry out the freezing of embedding sample, be convenient to operation, reduced complex operation in the past, not easy-operating shortcoming.
Claims (5)
1, the present invention is the organization embedding and refrigerating plant of a kind of Life Science Experiment that is used for and the check of medical Histopathology, is primarily characterized in that, is made up of multi-functional liquid nitrogen storage thermos, conical baffle, adjustable suspension waters case.
2, store thermos according to the described multi-functional liquid nitrogen of claim 1, be characterised in that: high 30cm, the cylindrical structural of end diameter 20cm, the design of top 15cm place has the handle that can be convenient for carrying.
3, according to claim 1,2 described multi-functional liquid nitrogen store thermos, it is characterized in that, thermos inner distance insulation bottleneck 10cm has designed one in the place and can allow adjustable suspension waters case to enter just, diameter is the conical plate washer of 10cm, it is freezing both can to have carried out investing tissue, again can be to reduce the volatilization of liquid nitrogen in the bottle.
4, according to claim 1,3 described adjustable suspension waters casees is characterized in that, high 15cm, the cylindrical structural of end diameter 10cm, the design of the both sides of waters case have two can free push-and-pull pull bar.
5, according to claim 1,3,4 described adjustable suspension waters casees is characterized in that, can regulate height and the position that is suspended from waters case in the thermos at adjustable suspension waters case two lateral bracing struts between 0～30cm by regulating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101956313A CN101451928A (en) | 2007-12-05 | 2007-12-05 | Multifunction organization embedding and refrigerating plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101956313A CN101451928A (en) | 2007-12-05 | 2007-12-05 | Multifunction organization embedding and refrigerating plant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101451928A true CN101451928A (en) | 2009-06-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007101956313A Pending CN101451928A (en) | 2007-12-05 | 2007-12-05 | Multifunction organization embedding and refrigerating plant |
Country Status (1)
| Country | Link |
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| CN (1) | CN101451928A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103543065A (en) * | 2013-10-14 | 2014-01-29 | 北京工业大学 | Ice nano-grade indentation sample bench and related experiment method |
| CN104655448A (en) * | 2015-02-08 | 2015-05-27 | 贵州大学 | Method for collecting hypophysis and hypothalamo of goat |
| WO2021026799A1 (en) * | 2019-08-14 | 2021-02-18 | 中国科学院深圳先进技术研究院 | Embedding mold and method of using same |
| CN112557159A (en) * | 2020-11-26 | 2021-03-26 | 华中科技大学同济医学院附属协和医院 | Specimen embedding device and frozen slicing method |
| CN113551457A (en) * | 2021-06-30 | 2021-10-26 | 艾佧科技(北京)有限公司 | Method for realizing homogeneity of photopolymerized resin embedded tissues by controlling polymerization temperature |
-
2007
- 2007-12-05 CN CNA2007101956313A patent/CN101451928A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103543065A (en) * | 2013-10-14 | 2014-01-29 | 北京工业大学 | Ice nano-grade indentation sample bench and related experiment method |
| CN103543065B (en) * | 2013-10-14 | 2016-01-20 | 北京工业大学 | A kind of ice body nano impress sample bench and related experimental methods |
| CN104655448A (en) * | 2015-02-08 | 2015-05-27 | 贵州大学 | Method for collecting hypophysis and hypothalamo of goat |
| WO2021026799A1 (en) * | 2019-08-14 | 2021-02-18 | 中国科学院深圳先进技术研究院 | Embedding mold and method of using same |
| CN112557159A (en) * | 2020-11-26 | 2021-03-26 | 华中科技大学同济医学院附属协和医院 | Specimen embedding device and frozen slicing method |
| CN112557159B (en) * | 2020-11-26 | 2023-11-07 | 华中科技大学同济医学院附属协和医院 | Specimen embedding device and frozen slicing method |
| CN113551457A (en) * | 2021-06-30 | 2021-10-26 | 艾佧科技(北京)有限公司 | Method for realizing homogeneity of photopolymerized resin embedded tissues by controlling polymerization temperature |
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Open date: 20090610 |