KR100406430B1 - Density concentrating device for examining the aquatic microalgae - Google Patents

Abstract

The present invention not only prevents the loss of microalgae in the sample but also reduces the time required while simplifying a series of operations such as the sample settling process and the removal of unnecessary supernatant, which is a pretreatment process for investigating the microalgae inhabiting the hydrosphere. The present invention relates to a density concentration device for irradiating microalgae that can be shortened.

The present invention is a device for concentrating the density so as to quantitatively examine the biomass of the microalgae contained in the sample, the sample is filled with a predetermined amount, the container 10 engraved on the outer surface, and the container 10 A cover 9 mounted on an open upper portion of the upper surface of the upper surface of the upper surface of the fixing hole 8, and the cover 9 is detachably mounted to the fixing hole 8 drilled on the upper surface thereof. A filter member 20 mounted at one end of the container 6 and disposed at a lower end of the container 10 and having a fine mesh 25 to prevent loss of the microalgae to be inspected, and within the container 10. A power unit 30 having a suction pump connected to the other end of the filtration tube 6 and the treated water transfer tube 32 to generate suction power so as to drain the filtered treated water to the collection container 31. It provides a density concentration apparatus for irradiating microalgae.

Description

DENSITY CONCENTRATING DEVICE FOR EXAMINING THE AQUATIC MICROALGAE}

The present invention not only prevents the loss of microalgae in the sample but also reduces the time required while simplifying a series of operations such as the sample settling process and the removal of unnecessary supernatant, which is a pretreatment process for investigating the microalgae inhabiting the hydrosphere. The present invention relates to a density concentration device for irradiating microalgae that can be shortened.

In general, the method of quantifying the sample taken to count the population of microalgae in the hydrosphere system is the amount of microalgae in a certain sample (1 L of seawater or fresh water). At this time, the sample is placed in a slide chamber (a slide chamber) that can contain a total of 1ml of the sample is used to count the number of microalgae microscopically.

However, in general, since the amount of microalgae contained in 1 L of the sample is very small, it is used as a pretreatment method to quantify the type by artificially increasing the density of the microalgae in the sample by removing the supernatant, which is a sample which is considered to be no microalgae.

In this pretreatment process, first, a sample containing aquatic microalgae is taken and placed in a container having a volume of 1 L in a cylindrical shape, and then a biofixed liquid such as formalin, a reagent for long-term storage of living organisms, is placed in the container. -3 days stationary (without moving the sample) to allow the microalgae to sink to the bottom of the vessel.

Subsequently, the operator performs a supernatant removal process that carefully removes 90% of the supernatant of the sample that has been settled in a container that is thought to be free of algae using a separate thin rubber tube. After counting the population of various microalgae in 10% of the sample, the value converted into the cell count per liter is used as the biomass value for each type.

However, during this series of pretreatments, the supernatant removal process that removes only 90% of the stationary samples from the stationary process requires skilled techniques, and the supernatant, which is a sample corresponding to the microalgae-free portion, is removed from the rubber tube. The sinking microalgae were likely to be sucked up together, so precise biomass values could not be obtained.

In addition, since only 10% of the samples left in the container can be left in the container and 90% of the supernatant cannot be removed accurately, if the amount of the supernatant is slightly higher than 90% by the rubber tube during the supernatant removal process, exactly 10% is desired. In order to match the residual amount of, there was a need to additionally add distilled water into the container by the amount of the excessively removed sample.

Accordingly, the present invention has been made to solve the above problems, the object of the present invention is to settle the microalgae in the sample and the supernatant, which is a pretreatment process for quantitative biomass investigation of aquatic microalgae in the hydrosphere ecosystem It is to provide a density concentration apparatus for microalgae inspection that can perform a series of operations such as the removal process simply and quickly, prevent the loss of microalgae in the sample, and reduce the work time.

1 is a block diagram showing a density concentration apparatus for irradiating microalgae according to the present invention,

Figure 2 is a perspective view showing a cover employed in the microalgae density density concentration apparatus according to the present invention,

Figure 3 is a perspective view showing a filter unit employed in the microalgae density density concentration apparatus according to the present invention,

Figure 4 is a state diagram showing the use of the density concentration device for irradiation of microalgae according to the present invention.

Explanation of symbols on the main parts of the drawings

6 ... Filtration tube 7 ... Fixture

8 ...... Fastener 9 ...... Cover

10 ..... Container 20 ..... Filter element

21 ..... Upper filter 22 ...... Male thread

23 ..... Lower filter 24 ...... Female thread

30 ..... Power unit 31 ...... Collecting vessel

The present invention as a technical means for achieving the above object,

In the device for concentrating the density so that the biomass of the microalgae contained in the sample can be inspected regularly,

The sample is filled in a predetermined amount, the container is engraved on the outer surface, and the fastener is mounted on the open upper portion of the container, and the fastener in which the filter tube is inserted in a movable hole in the upper surface is detachably mounted. A filter member disposed at a lower end of the container, attached to one end of the filter tube, and having a fine mesh to prevent loss of microalgae to be inspected, and draining the treated water filtered in the container to a collection container. And a power unit having a suction pump connected in communication with the other end of the filter tube and the treatment water moving tube to generate a suction force, wherein the fixture is press-fitted into the fixing hole to securely fix the filter tube. Microalgae characterized by consisting of a truncated conical rubber member having a larger upper outer diameter than the inner diameter of the fixing hole, the lower outer diameter is smaller By providing a density concentrating device for irradiation.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

1 is a block diagram showing a density concentration apparatus for irradiating microalgae according to the present invention, Figure 2 is a perspective view showing a cover employed in the density concentration apparatus for microalgae irradiation according to the present invention, Figure 3 is a present invention As a perspective view showing the filter unit employed in the microalgae density density device according to the present invention, the apparatus 1 of the present invention is a pretreatment for counting the population of microalgae (plankton) inhabiting the marine and freshwater ecosystem In order to increase the density of the microalgae necessary in the process, by removing the microalgae not included in the microalgae by the stationary process, such an apparatus 1 includes a container 10, a cover 9, a filter member 20 and a power unit ( 30).

That is, the container 10 is made of a hollow cylindrical shape of which the upper part is open and the lower part is closed to fill a predetermined amount of the sample containing the microalgae to be examined, and the outer surface of the container to accurately know the capacity of the sample to be filled. The scale is engraved.

In addition, the cover 9 mounted on the open top of the container 10 has a circular fixed hole 8 formed in the upper surface thereof, and the filtration tube 6 is inserted into the insertion hole 7a so as to be movable by an external force. The fastener 7 which inserted () is detachably attached.

Here, the fastener 7 has a size larger than the inner diameter of the fixing hole 8 and the lower outer diameter is smaller than the inner diameter of the fixing hole 8 so as to be firmly fixed in the fixing hole (8) It is better to consist of a truncated conical rubber member.

In addition, the filter member 20 mounted on one end of the filtration tube 6 positioned to be disposed near the lower end of the container 10 includes an upper filter 21 having a male screw portion 22 formed on an outer peripheral surface of the lower end. And a lower filter 23 formed on an inner circumferential surface of an upper end of the female screw part 24 fastened to the male screw part 22, and a microalgae to be inspected is disposed on the lower surface of the lower filter 23. Equipped with a fine mesh 25 having a net shape smaller than the size of the microalgae so that the supernatant in the discharge to be drained through the filtration tube 6 to the collection vessel 31 to be described later.

Here, when the size of the microalgae to be examined increases or decreases, the pretreatment process may be continued by replacing the lower filter 23 having the micromeshes 25 of various sizes so as to prevent loss of the microalgae. .

Meanwhile, as shown in FIG. 4, the power unit 30 mediates the filtration tube 6 and the water treatment moving tube 32 so that only the supernatant liquid left in the container 10 can be drained to the collecting container 31 side. A suction pump (not shown) configured to be connected in communication with each other to provide suction power is configured, and the power unit 30 and the collection container 31 are connected to the connection pipe 33.

The operation of the present invention as described above will be described.

First, the sample containing the microalgae to be inspected in the vertical container 10 is filled with a predetermined amount while looking at the scale formed on the outer surface of the container 10, and then the upper part of the open container 10 is used as the cover 9. Closed,

Then, when the operator forcibly presses the fixture 7 into which the filter tube 6 is inserted into the insertion hole 7a to be inserted into the fixing hole 8 of the cover 9, the fixture 7 made of a rubber member Is fixed while being fixed within the fixing hole (8) and at the same time to shrink the insertion hole (7a) to firmly secure the filtration tube (6). As a result, the filter member 20 attached to the lower end of the filtration tube 6 is located near the lower end in the container 10.

Here, when the position of the filter member 20 is changed in the vertical direction to freely adjust the desired final quantity in the container 10, the filtration tube 6 assembled to the fixture 7 is inserted into the hole ( 7a) because it is movable up and down, the filter tube 6 is pushed upwards or lowered downwards in the state in which the fixture 7 inserted into the fixing hole 8 is separated outward. By adjusting the length of the filtration tube 6 between the filter member 20 and the position of the filter member 20 in the container 10.

Then, when the suction pump (not shown) of the power unit 30 connected through the rear end of the filtration tube 6 and the treated water transfer tube 32 is operated, the tip of the filtration tube 6 in the vessel 10 is operated. The suction force is generated to force the sample to be forcibly sent to the collection vessel 31 through the filtration tube 6, the treated water transfer tube 32, and the connecting tube 33.

Here, because the microalgae contained in the sample is filtered by the micro mesh 25 provided to the lower filter 23 of the filter member 20, the microalgae having a larger particle size than the micro mesh 25 Is not drained to the collection vessel side through the filtration tube 6, it remains intact under the container 10, and is filtered by the fine mesh 25 to drain only the sample without microalgae to the collection vessel 31. To be processed.

Therefore, due to the microalgal filtration treatment as described above, only the final water containing the microalgae filtered by the micromesh 25 is exactly as desired in the lower portion of the container 10 corresponding to the lower portion of the filter member 20. Remaining. Accordingly, the worker can take out the cover 9 and put the final water in another container to accurately and precisely examine the number of microalgae per unit volume.

On the other hand, as shown in Fig. 4, by mounting each of the filter tubes having a filter member in the plurality of vessels 10, and connecting the respective filter tubes to the treated water transfer pipe of the power unit, fine in the plurality of vessels at the same time It is possible to improve the throughput per unit time simply by performing a pretreatment process to filter the sample without algae.

With the present invention having the above-described configuration, since the scale is displayed on the outer surface of the container, only the sample containing the microalgae can be accurately retained while draining the sample containing the microalgae correctly into the collection container. In addition, since the micro mesh that is not able to escape the microalgae is mounted on the filtration member, it is possible to prevent the loss of the microalgae in the sample filtration process, thereby improving the inspection accuracy as compared to the conventional one, and the shangdong of the filtration tube having the filter member is possible. Therefore, the final quantity can be freely adjusted as desired, thereby obtaining the effect of simplifying the density concentration of the microalgae.

In addition, after the biofixing solution is added into the sample, the sample is allowed to stand for 2 to 3 days, and thus, the stationary process of sinking the microalgae on the bottom surface is unnecessary, and thus, the work productivity can be remarkably improved as compared with the conventional method.

Claims (3)

In the device for concentrating the density so that the biomass of the microalgae contained in the sample can be quantitatively inspected, The sample 10 is filled with a predetermined amount, and the filter tube 6 is mounted on the container 10 engraved on the outer surface, and the fixing hole 8 mounted on the open upper part of the container 10 and perforated on the upper surface. Cover 9 which is movably inserted fastener 7 is detachably mounted, and is attached to one end of the filtration tube 6 and disposed at the lower end of the container 10, and loss of microalgae to be inspected. Filter member 20 having a fine mesh 25 to prevent the, and the other end of the filter tube 6 and the treated water transfer pipe to drain the treated water filtered in the container 10 to the collection container 31 ( And a power unit 30 having a suction pump connected in communication with each other via a medium 32 to generate a suction force. The fastener 7 is press-fitted into the fixing hole 8 to firmly hold the filtration tube 6. A truncated cone having a larger upper outer diameter and lower outer diameter than the inner diameter of the fixing hole 8 so as to be fixed. Density concentrating device for microalgae irradiation, characterized in that consisting of a rubber type member. delete The method of claim 1, The filter member 20 is formed on the upper inner peripheral surface of the upper filter 21 having the male screw portion 22 formed on the outer peripheral surface of the lower end, and the female screw portion 24 fastened to the male screw portion 22, and the fine mesh ( Density concentrator for irradiating microalgae, characterized in that consisting of a lower filter (23) having a 25).