JP2008286708A - Device and method for sampling aquatic organism - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a device and method for sampling an aquatic organism, capable of collecting and enriching the micro aquatic organism contained in water under a living condition, without damaging an individual living organism. <P>SOLUTION: The device is installed with filter cloth 3 having an upper end opening 3a and a lower end opening 3b, and for partitioning an inside of a water storage container 1 into two outer and inner spaces 1A, 1B, and a collection container 4 for collecting the aquatic organism into the lower end opening 3b of the filter cloth 3, in the water storage container 1 capable of regulating storage water W1 in its inside to brought into a fixed water amount, the storage water W1 is stored preliminarily in the inside of the water storage container 1, in a degree of exposing the upper end opening 3a of the filter cloth 3, water W2 containing the aquatic organism of a sampling object is supplied from the upper end opening 3a into an inside of the filter cloth 3, and the water W2 containing the aquatic organism remaining inside the filter cloth 3 is collected thereafter by draining the storage water W1 existing outside the filter cloth 3 in the inside of the water storage container 1. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an aquatic organism sampling apparatus and an aquatic organism sampling method, and more particularly to an aquatic organism sampling apparatus and an aquatic organism sampling method capable of collecting and concentrating aquatic organisms contained in water alive. .

  Conventionally, when collecting aquatic organisms such as plankton that live in the water, attach a filter cloth to the tip of the rope and pull it underwater to collect aquatic organisms adhering to the filter cloth, or a bucket or other container at the tip of the rope The aquatic organisms are concentrated by collecting a predetermined amount of water by filtering and filtering the water.

Patent Document 1 discloses that water pumped up by a pump is poured into a column in which a carrier is inserted, and microorganisms in the water are concentrated and collected on the carrier.
JP 2003-144134 A

  When collecting minute aquatic organisms that live in water, there is a demand to collect and concentrate the target aquatic organisms alive. For example, in the case of collecting and concentrating aquatic organisms such as plankton of a desired size fraction from the water for the purpose of test research in a research institution such as a university or a fisheries experimental station. It is also required for exploration of foreign organisms in cultivated fisheries and culture.

  However, in any of the above conventional methods, the collected aquatic organisms may be strongly pressed against the inner surface of the filter cloth or the carrier during filtration for concentration, and the cells may be damaged and killed. Therefore, there is a problem that it is extremely difficult to collect and concentrate the organisms in the water in a highly active state. In addition, when cells are damaged and damaged, there is a possibility that the cell density of an organism cannot be evaluated correctly.

  Accordingly, an object of the present invention is to provide an aquatic organism sampling apparatus and an aquatic organism sampling method that can be collected and concentrated while alive without damaging a small aquatic organism individual contained in water. .

  The above problems are solved by the following inventions.

(Claim 1)
A water storage container that is stored so that the stored water can be adjusted to a constant water volume;
A filter cloth having an upper end opening and a lower end opening, immersed in the stored water in the water storage container so that the upper end opening is exposed, and dividing the water storage container into two spaces, an outer side and an inner side;
A drain outlet for draining stored water outside the filter cloth in the water storage container;
A collecting container that is provided at the lower end opening of the filter cloth and collects aquatic organisms by storing water containing aquatic organisms inside the filter cloth when the stored water in the water storage container is drained; Aquatic organism sampling device characterized by

(Claim 2)
A filter cloth having an upper end opening and a lower end opening in a water storage container that can be adjusted so that the amount of water stored in the inside is constant, and partitioning the inside of the water storage container into an outer space and an inner space, and the filter cloth A collecting container for collecting aquatic organisms at the lower end opening of the water storage tank, and storing the stored water in the water storage container in advance to such an extent that the upper end opening of the filter cloth is exposed, and the inner side of the filter cloth After supplying water containing aquatic organisms to be sampled from the upper end opening, water containing aquatic organisms remaining inside the filter cloth is drained by draining the stored water outside the filter cloth in the water storage container. Is collected in the collection container.

  ADVANTAGE OF THE INVENTION According to this invention, the aquatic organism sampling apparatus and the aquatic organism sampling method which can be extract | collected alive and concentrated, without damaging the living individual of the micro aquatic organism contained in water can be provided.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

  FIG. 1 is a schematic view showing an example of the aquatic organism sampling apparatus according to the present invention.

  In the figure, reference numeral 1 denotes a water storage container, which is formed in a cylindrical shape, for example, in which stored water W1 is stored. The water level of the stored water W1 is determined by the position of the upper end opening 2a of the overflow pipe 2.

  From the viewpoint of maintaining the habitat environment of aquatic organisms in the water storage container 1, it is preferable to use the sampling target water as the stored water W1. The stored water W1 is supplied into the water storage container 1 from a supply port (not shown) provided in the water storage container 1.

  A filter cloth 3 for collecting aquatic organisms to be collected is immersed in the water storage container 1. The filter cloth 3 has an upper end opening 3a and a lower end opening 3b. Preferably, the filter cloth 3 is formed in a substantially inverted conical shape that is tapered toward the lower end opening 3b from the upper end opening 3a. It is provided in the stored water W1 so as to be exposed above the water level of the water W1. Thereby, the inside of the water storage container 1 is partitioned into a space 1 </ b> A outside the filter cloth 3 and a space 1 </ b> B inside the filter cloth 3. The upper end opening 2 a of the overflow pipe 2 is located below the upper end opening 3 a of the filter cloth 3 and above the lower end opening 3 b of the filter cloth 3.

  The mesh of the filter cloth 3 is determined according to the size of the aquatic organism to be collected. For example, when aquatic organisms having a size exceeding 50 μm are collected, the aquatic organism is formed to have a thickness of 50 μm.

  A collection container 4 is connected to the lower end opening 3 b of the filter cloth 3. The collection container 4 is a container for collecting aquatic organisms by storing water (concentrated water) containing aquatic organisms finally remaining inside the filter cloth 3 in the water storage container 1, and using a synthetic resin or the like, For example, it is formed in a funnel shape that is sufficiently smaller than the water storage container 1. A water collection pipe 5 that is opened and closed by an on-off valve 6 is connected to the bottom of the collection container 4. By opening the on-off valve 6, water containing aquatic organisms stored and collected in the collection container 4 (concentration) Water) can be collected outside the water storage container 1.

  A drain port 7 is formed at the bottom of the water storage container 1, and the internal stored water W 1 can be drained by opening the on-off valve 8.

  Next, an aquatic organism sampling method using such a sampling device will be described with reference to FIGS.

  First, as shown in FIG. 2, the sampling target water W2 including the aquatic organism to be collected is filtered into the space 1B inside the filter cloth 3 in the water storage container 1 in which the stored water W1 is stored. From the upper end opening 3a.

  When the sampling target water W2 is supplied to the inside of the filter cloth 3, the amount of water stored in the water storage container 1 increases, but the increased amount overflows from the overflow pipe 2 and is discharged to the outside of the water storage container 1. The inside of 1 is always kept constant. For this reason, when the sampling target water W2 is supplied to the inside of the filter cloth 3, the surplus water moves from the inside to the outside of the filter cloth 3, and the sampling target that cannot pass through the filter cloth 3 in the filter cloth 3 The amount of aquatic organisms of the size increases.

  At this time, since the stored water W1 is initially stored in the water storage container 1, the aquatic organisms contained in the sampling target water W2 supplied to the inside of the filter cloth 3 are suddenly filtered and applied to the filter cloth 3. The aquatic organisms are not killed because they are not pressed and are prevented from being damaged by the filter cloth 3 to the aquatic organisms.

  After supplying the sampling target water W2 to the inside of the filter cloth 3, when the on-off valve 8 is opened and the stored water W1 in the water storage container 1 is drained from the drain port 7, the stored water in the space 1A outside the filter cloth 3 is stored. The sampling target water W2 in the space 1B inside the filter cloth 3 as well as W1 moves from the inside of the filter cloth 3 to the outside and is drained. Accordingly, aquatic organisms that cannot pass through the filter cloth 3 remain in the filter cloth 3.

  When drainage of the stored water W1 in the water storage container 1 is completed, or when the water level of the stored water W1 is drained to a level lower than that of the collection container 4, as shown in FIG. Only the concentrated water W3 in which the organism is concentrated is stored in the collection container 4. Since the concentrated water W3 concentrates aquatic organisms without substantially applying a pressure filtered by the filter cloth 3, the aquatic organisms are concentrated alive without being damaged. The concentrated water W3 in the collection container 4 is taken out of the water storage container 1 from the water collection pipe 5 as necessary.

  As described above, according to the aquatic organism sampling apparatus and the sampling method according to the present invention, it is possible to collect and concentrate alive without damaging the aquatic organism individual contained in the sampling target water. . 4 to 7 show the state of aquatic organisms in concentrated water obtained by the aquatic organism sampling apparatus and sampling method according to the present invention. The concentrated water is concentrated in a normal state where the aquatic organisms are alive. You can see that

Schematic showing an example of an aquatic organism sampling apparatus according to the present invention The figure explaining the sampling method of the aquatic organism which concerns on this invention The figure explaining the sampling method of the aquatic organism which concerns on this invention The photograph which shows the mode of the aquatic organism in the concentrated water obtained by the sampling apparatus and sampling method of the aquatic organism concerning this invention The photograph which shows the mode of the aquatic organism in the concentrated water obtained by the sampling apparatus and sampling method of the aquatic organism concerning this invention The photograph which shows the mode of the aquatic organism in the concentrated water obtained by the sampling apparatus and sampling method of the aquatic organism concerning this invention The photograph which shows the mode of the aquatic organism in the concentrated water obtained by the sampling apparatus and sampling method of the aquatic organism concerning this invention

Explanation of symbols

1: Water storage container 1A: Space outside filter cloth 1B: Space inside filter cloth 2: Overflow pipe 2a: Upper end opening 3: Filter cloth 3a: Upper end opening edge 3b: Lower end opening edge 4: Collection container 5: Water sampling pipe 6: Open / close valve 7: Drain port 8: Open / close valve

Claims (2)

A water storage container that is stored so that the stored water can be adjusted to a constant water volume;
A filter cloth having an upper end opening and a lower end opening, immersed in the stored water in the water storage container so that the upper end opening is exposed, and dividing the water storage container into two spaces, an outer side and an inner side;
A drain outlet for draining stored water outside the filter cloth in the water storage container;
A collecting container that is provided at the lower end opening of the filter cloth and collects aquatic organisms by storing water containing aquatic organisms inside the filter cloth when the stored water in the water storage container is drained; Aquatic organism sampling device characterized by   A filter cloth having an upper end opening and a lower end opening in a water storage container that can be adjusted so that the amount of water stored in the inside is constant, and partitioning the inside of the water storage container into an outer space and an inner space, and the filter cloth A collecting container for collecting aquatic organisms at the lower end opening of the water storage tank, and storing the stored water in the water storage container in advance to such an extent that the upper end opening of the filter cloth is exposed, and the inner side of the filter cloth After supplying water containing aquatic organisms to be sampled from the upper end opening, water containing aquatic organisms remaining inside the filter cloth is drained by draining the stored water outside the filter cloth in the water storage container. Is collected in the collection container.