CN217738796U - Liquid-based thin-layer cell film-making dyeing device - Google Patents

Abstract

The utility model relates to the technical field of medical equipment, in particular to a liquid-based thin-layer cell slide-making and dyeing device, which comprises a specimen pretreatment device, a cell transfer device and a specimen dyeing device which are arranged in sequence along a dyeing slide-making process; the cell transfer device comprises a suction head with openings at two axial ends and a positive and negative pressure generating device communicated with the suction head through an opening at the upper end of the suction head, and a filter membrane is arranged at an opening at the lower end of the suction head. The utility model provides a liquid-based thin-layer cell film-making dyeing apparatus is convenient for operate and diagnosis cell enrichment is efficient and the cell integrity is high.

Description

Liquid-based thin-layer cell film-making dyeing device

Technical Field

The utility model belongs to the technical field of medical equipment, in particular to liquid-based thin-layer cell film-making dyeing apparatus.

Background

The extraction of human exfoliated cells and observation of the morphological and structural changes thereof under a microscope are important means for diagnosing human diseases, and the process of uniformly spreading a human exfoliated cell monolayer on a glass slide and then dyeing is one of the key processes. Film-making and dyeing machines mostly use the membrane tube filtration (TCT) and the natural sedimentation (LCT).

LCT is organically combined with density gradient reagent and centrifugation to separate the sample liquid and enrich (concentrate) more than 92% of effective diagnostic cells. According to the principle of pathological cell nucleus serous ratio increase and specific gravity increase, the cells are distinguished according to specific gravity. The gravity natural sedimentation method is used for flaking, the diagnosis cells fall on the glass slide from top to bottom, the probability of catching pathological cells is increased, and meanwhile, the natural shape of the cells is guaranteed. TCT uses filters to differentiate cells by their physical size (volume) and is a random access of cells. In the negative pressure suction and tabletting process of TCT, external force acts on cells, so that cell membranes are easily damaged, and cytological diagnosis such as fluorescent staining cannot be performed.

LCT has advantages over TCT in sampling procedures and preservation solution use. The data show that discarding the brush lost 37% of the diagnostic cells, indicating that LCT collected more total diagnostic cells than TCT. The main component of the preservation solution used by LCT is low-concentration ethanol, the main component of the preservation solution used by TCT is high-concentration methanol, and the cover must be opened in the tabletting process, so that pollution is easily caused, and the waste liquid is difficult to treat.

In addition, the number and integrity of the LCT capturing pathological cells are better than that of TCT. However, LCT also has significant drawbacks based on the low level of automation of current instruments: the components and the quantity of the components settled on the glass slide cannot be controlled, the depth of field under a sample lens is large, cells are overlapped, and meanwhile, interference components such as mucus, tissue blocks, debris, impurities and the like still exist, so that the cells are difficult to observe and diagnose under a microscope, the reading difficulty is increased, the working efficiency is influenced, and missed diagnosis and misdiagnosis are easy to occur; the whole flaking process adopts two times of specific gravity liquid gradient centrifugation, and then flaking and dyeing are carried out, so that the whole technical process is tedious and tedious. In addition, the defects of high experience requirement on operators, high labor intensity, complex consumable components, high equipment manufacturing cost and the like exist. In addition, the specimen needs to be transferred among a specimen bottle, a centrifuge tube and a staining tank for many times, and particularly, the specimen for the hydrothorax and ascites is treated, so that a great biological risk exists.

Therefore, there is a need for a new sample enrichment device that can eliminate the tedious shaking, plating and centrifugation of LCT and can enrich more complete diagnostic cells than TCT.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects of the prior art, the utility model aims to solve the technical problems that: provides a liquid-based thin-layer cell slice-making and dyeing device which is convenient to operate, high in diagnostic cell enrichment efficiency and high in cell integrity.

In order to solve the technical problem, the utility model discloses a technical scheme be: a liquid-based thin-layer cell slice-making dyeing device comprises a specimen pretreatment device, a cell transfer device and a specimen dyeing device which are sequentially arranged along a dyeing slice-making procedure; the cell transfer device comprises a suction head with openings at two axial ends and a positive and negative pressure generating device communicated with the suction head through an opening at the upper end of the suction head, and a filter membrane is arranged at an opening at the lower end of the suction head.

Wherein the specimen pretreatment device comprises a specimen filter;

the specimen filter includes a body cup and a filter bowl nested within the body cup;

the side wall of the filter cup and the side wall of the main body cup are enclosed to form a sampling cavity, and a sampling port communicated with the sampling cavity is formed in the main body cup;

the bottom of the filter bowl is provided with an opening and an inclined-plane-shaped filter screen, and the inner cavity of the filter bowl is communicated with the sampling cavity through the bottom opening of the filter bowl.

Wherein the mesh number of the filter screen is 40-120 meshes.

The specimen pretreatment device further comprises a first placing frame;

the main body cup is provided with at least one positioning groove formed by inwards sinking;

the first placing frame is provided with a groove for accommodating the specimen filter and a protrusion which is arranged in the groove and matched with the positioning groove.

Wherein, cell transfer device include the triaxial arm, and with the host computer that the triaxial arm links to each other, mount pad, pivot and driving motor have in the host computer, driving motor passes through the pivot with the mount pad transmission is connected, have in the mount pad and be used for connecting the suction nozzle of suction head.

Wherein the positive and negative pressure generating device forms a positive pressure of 5-24 kPa or a negative pressure of-24 to-20 kPa in the suction head.

The cell transfer device further comprises a recovery box, an unloading sucker frame, a cleaning groove and a second placing frame for loading suckers.

The unloading headstock is provided with a material-unloading port located right above the recovery box.

Wherein, the specimen staining device is an LCT staining device.

The specimen dyeing device comprises a YZ-axis mechanical arm, a dyeing host connected with the YZ-axis mechanical arm, a dyeing tank and a third placing rack;

the dyeing host comprises a dyeing needle;

the dyeing tank is arranged on the third placing frame and is positioned below the dyeing needle.

The beneficial effects of the utility model reside in that: the utility model provides a liquid-based thin layer cell film-making dyeing apparatus accessible specimen pretreatment device carries out the preliminary treatment to the sample, filter the impurity in the sample promptly, cell transfer device takes place the device through its positive negative pressure simultaneously in order to form malleation or negative pressure in the suction head, thereby the realization adsorbs the diagnostic cell of preliminary treatment on the filter membrane from specimen pretreatment device, and in the immigration sample dyeing apparatus, whole operation process is succinct, degree of automation is high, can effectively improve the efficiency of film-making dyeing. Meanwhile, the diagnostic cells are collected and released in a filter membrane mode, the quality and the quantity of the diagnostic cells can be effectively improved, the cells are uniformly distributed on a glass slide under a microscope, the cell overlapping and the slide cavity are few, the background interference is small, and the diagnostic efficiency and the diagnostic accuracy are improved.

Drawings

FIG. 1 is a schematic diagram of a liquid-based thin-layer cell slide-making and staining apparatus according to an embodiment of the present invention;

fig. 2 is an exploded view of a specimen pretreatment device according to an embodiment of the present invention;

FIG. 3 is a schematic view of a specimen pretreatment device (without a cup cover) at a viewing angle according to an embodiment of the present invention;

FIG. 4 shows a side cross-sectional view of a bowl in an embodiment of the present invention;

fig. 5 is a schematic structural view of a first rack according to an embodiment of the present invention;

FIG. 6 is a schematic view of a cell transfer device according to an embodiment of the present invention from a viewing angle;

FIG. 7 is a cross-sectional view of a cell transfer device according to an embodiment of the present invention;

FIG. 8 is an enlarged view of portion A of FIG. 1;

fig. 9 is a schematic structural view of a specimen staining apparatus according to an embodiment of the present invention;

FIG. 10 is a photograph showing the specimen staining under the 40 Xmicroscope of the conventional LCT method in the experimental example of the present invention;

FIG. 11 is a photograph showing the staining of a specimen by the conventional LCT method according to the present invention in an experimental example;

FIG. 12 is a photograph showing the staining of a specimen under New Berth (TCT) 40 Xscope in the experimental example of the present invention;

FIG. 13 is a photograph showing the staining of a specimen by New Berth (TCT) in the experimental example of the present invention;

fig. 14 is a photograph showing the specimen staining under the 40x lens of the liquid-based thin layer cell slide-making staining apparatus provided by the present application in the experimental example;

fig. 15 is a photograph showing a specimen staining of the liquid-based thin layer cell slide-making staining apparatus according to the present invention in an experimental example.

Description of the reference symbols: 1. a cell transfer device; 11. a three-axis mechanical arm; 12. a host; 121. a drive motor; 122. a rotating shaft; 123. a suction nozzle; 13. removing the suction head frame; 131. a material removing port; 14. a recovery box; 15. a cleaning tank; 16. a second rack;

2. a specimen pretreatment device; 21. a main body cup; 211. positioning a groove; 22. a filter bowl; 221. filtering with a screen; 23. A cup cover; 24. a sampling port; 25. a sampling cavity; 26. a first placing rack; 261. a groove; 262. a protrusion;

3. a specimen staining device; 31. a YZ-axis mechanical arm; 32. dyeing needle; 33. a third rack; 34. a dyeing tank;

4. a suction head; 41. and (5) filtering the membrane.

Detailed Description

In order to explain the technical contents, the objects and the effects of the present invention in detail, the following description is made with reference to the accompanying drawings in combination with the embodiments.

A liquid-based thin-layer cell slice-making dyeing device comprises a specimen pretreatment device, a cell transfer device and a specimen dyeing device which are sequentially arranged along a dyeing slice-making procedure; the cell transfer device comprises a suction head with openings at two axial ends and a positive and negative pressure generating device communicated with the suction head through an opening at the upper end of the suction head, and a filter membrane is arranged at an opening at the lower end of the suction head.

As shown in fig. 1, the specimen pretreatment apparatus 2 is mainly used for filtering a specimen to decompose or filter out interfering components such as large cell aggregates, blood, and mucus mixed in a specimen solution. However, during the filtration, the ratio of cell nuclei of the pathological changes increases, and the specific gravity thereof increases, so that the pathological diagnostic cells are easily deposited on the bottom of the cup for accommodating the filtered diagnostic cells in the specimen pretreatment apparatus 2. Therefore, before the diagnostic cells are collected by the cell transfer device 1, positive pressure (air inflation) is required to be formed in the suction head 4 by the positive and negative pressure generating device thereof to promote the diagnostic cells to be uniformly dispersed in the dispersion medium (preservation solution), negative pressure (air suction) is formed in the suction head 4 by the positive and negative pressure generating device at this time to adsorb the diagnostic cells uniformly dispersed in the dispersion medium on the filter 41 on the end surface of the suction head 4, the filter 41 on the end surface of the suction head 4 is lifted off the liquid surface of the dispersion medium and then transferred to the specimen staining device 3, positive pressure is formed inside the suction head 4 by the positive and negative pressure generating device at this time to blow the diagnostic cells adhered on the filter 41 thereof into a cup for loading with staining solution, and then cell staining is performed, thereby realizing the pretreatment-transfer-staining step for the diagnostic cells.

In one embodiment, referring to fig. 2-4, the specimen pre-processing device 2 includes a specimen filter; the specimen filter includes a body cup 21 and a filter cup 22 nested within the body cup 21; the side wall of the filter cup 22 and the side wall of the main body cup 21 enclose to form a sampling cavity 25, and a sampling port 24 communicated with the sampling cavity 25 is arranged on the main body cup 21; the bottom of the filter bowl 22 is provided with an opening, the opening is provided with a filter screen 221 in an inclined plane shape, and the inner cavity of the filter bowl 22 is communicated with the sampling cavity 25 through the bottom opening of the filter bowl 22.

Wherein the filter bowl 22 is primarily adapted to receive a cell collection brush. By placing the cell collection brush in the filter cup 22 and immersing the collection head of the cell collection brush in a preservation solution poured into the main body cup 21 in advance, diagnostic cells, large cell masses, blood, mucus and the like attached to the collection head are caused to be dissolved and dispersed in the preservation solution by the combined action of the preservation solution and mechanical oscillation, and a part of interfering components are decomposed. And since the specific gravity of the diagnostic cells is large, the diagnostic cells may pass through the mesh 221 provided at the bottom of the bowl 22 into the body cup 21 during sedimentation thereof, while most of the interfering components are trapped by the mesh 221. Preferably, the mesh number of the filter screen 221 is 40 to 120 meshes. Of course, in order to avoid the preservation liquid from splashing out of the filter cup 22 or the sampling port 24 during the mechanical oscillation process, it is also allowable to arrange the cup cover 23 at the opening of the main body cup 21, and for the convenience of installing the cup cover 23, the cup cover 23 and the main body cup 21 are preferably connected by screw threads.

After the diagnostic cells are filtered, the cup cover 23 is opened, and the end surface of the suction head 4 extends into the sampling cavity 25 through the sampling port 24 to collect the diagnostic cells.

During the automated operation, the position of the sampling port 24 determines whether the pipette tip 4 can stably collect diagnostic cells. Thus, in one embodiment, referring to fig. 5, the specimen pre-processing apparatus 2 further includes a first rack 26; the main body cup 21 is provided with at least one positioning groove 211 formed by inwards sinking; the first holder 26 has a recess 261 for accommodating the specimen filter, and a projection 262 disposed in the recess 261 and matching with the positioning groove 211. Namely, the projection 262 arranged in the positioning groove 211 is matched with the positioning groove 211 arranged on the side wall of the main body cup 21, so as to fix the position of the sampling port 24. Preferably, the specimen pre-treatment device 2 employs a sample collection vial as provided in patent CN113310759a, and is hereby incorporated by reference in its entirety.

Referring to fig. 6 and 7, the cell transfer device 1 includes a three-axis robot 11, and a host 12 connected to the three-axis robot 11, the host 12 has a mounting seat therein, a rotating shaft 122, and a driving motor 121, the driving motor 121 is in transmission connection with the mounting seat through the rotating shaft 122, and the mounting seat has a suction nozzle 123 therein for connecting the suction head 4.

Preferably, the positive and negative pressure generating device forms a positive pressure of 5 to 24kPa or a negative pressure of-24 to-20 kPa in the suction head 4. The triaxial mechanical arm 11 is used for freely driving the main machine 12 to move in X, Y, Z triaxial. In particular, the upper part of the suction head 4 is open, so that the suction head 4 can be attached by inserting the suction nozzle 123 into its opening. The suction nozzle 123 is connected to the positive/negative pressure generating device through a pipeline, and a liquid storage tank is also connected to the pipeline. That is, when the three-axis robot arm 11 drives the suction head 4 to extend into the sampling cavity 25 through the main machine 12, the positive and negative pressure generating device is used for pumping air to form negative pressure in the suction head 4, so as to adsorb diagnostic cells dispersed in the preservation solution onto the filter membrane 41 on the other end surface of the suction head 4, and simultaneously suck part of the preservation solution into the liquid storage tank. When the filter membrane 41 on the end surface of the suction head 4 is separated from the liquid level of the preservation solution, or is separated from the sampling port 24, or in the process that the three-axis mechanical arm 11 moves on the X axis, the driving motor 121 drives the suction head 4 to rotate by a certain angle in the vertical direction through the rotating shaft 122, or to swing within a certain range, so that the preservation solution absorbed in the filter membrane 41 or the redundant preservation solution adhered to the inner wall of the suction head 4 is further sucked into the liquid storage tank, and the pollution of the preservation solution to the dyeing solution in the subsequent dyeing process is avoided. The material of the tip 4 is preferably PET, and the pore diameter of the filter 41 is preferably 4 to 10 μm. Preferably, the cell transfer device 1 is the cell transfer device 1 provided in patent CN113324824a, and is hereby incorporated by reference in its entirety. At the same time, the density of the filter membrane 41 (the pore size of the filter pores) should be lower than the density of the filter screen 221, so that during the enrichment of the diagnostic cells in the filter membrane 41, non-diseased cells can pass through the filter membrane 41 to retain as many diagnostic cells as possible on the surface of the filter membrane 41.

Further, referring to fig. 1 and 8, the cell transfer apparatus 1 further includes a recovery box 14, a discharge tip rack 13 and a wash bowl 15, and a second rack 16 for loading the tips 4, wherein the discharge tip rack 13 has a discharge port 131 directly above the recovery box 14.

In particular, unused tips 4 are typically loaded into the second rack 16. After the specimen is transferred, the three-axis robot arm 11 moves the tip 4 to the tip discharging rack 13, and the tip 4 is taken off the suction nozzle 123 through the discharging port 131 by the action of the three-axis robot arm 11 slightly lifted upward, the tip 4 drops into the recovery box 14, and then the three-axis robot arm 11 immerses the suction nozzle 123 into the cleaning tank 15 loaded with the purified water in advance, and the purified water is sucked into the liquid storage tank through the suction nozzle 123 by the positive and negative pressure generating device, and the suction nozzle 123 and the internal pipeline can be cleaned by the purified water in the process. Finally, the suction nozzle 123 is transferred to the second rack 16 again and the suction head 4 is reconnected to wait for the processing procedure of moving down the specimen.

The specimen staining apparatus 3 is any commercially available LCT staining apparatus.

Preferably, referring to fig. 9, the specimen staining apparatus 3 includes a YZ-axis robot arm 31, a staining host connected to the YZ-axis robot arm 31, a staining bath 34, and a third rack 33; the dyeing host comprises a dyeing needle 32; the dyeing tank 34 is disposed on the third rack 33, and the dyeing tank 34 is located below the dyeing needle 32. Namely, the dyeing needle 32 is driven to move by the YZ shaft mechanical arm 31 and is matched with liquid path control to realize the drop dyeing of the specimen (diagnosis cell).

The utility model provides a liquid-based thin-layer cell film-making dyeing apparatus not only can be used for the exfoliated cell film-making of cervix uteri and vagina, also can use in other fields, the preparation of sputum, urine, ascites in chest, cerebrospinal fluid, bronchus lavage liquid and various fine needle puncture samples. The staining method and the staining device do not need to carry out oscillation, liquid adding and centrifugation on the sample, do not need to manually transfer the sample among the sample bottle, the centrifuge tube and the staining tank 34 for many times, and greatly reduce the biological risk. And at the same time, the automation degree is easy to realize.

Example 1

Referring to fig. 1 to 9, a liquid-based thin-layer cell slide-making staining apparatus includes a specimen pretreatment apparatus 2, a cell transfer apparatus 1, and a specimen staining apparatus 3, which are sequentially disposed along a slide-making staining process; the cell transfer device 3 comprises a suction head 4 with openings at two axial ends and a positive and negative pressure generating device communicated with the suction head through an opening at the upper end of the suction head 4, and a filter membrane 41 is arranged at an opening at the lower end of the suction head 4;

the specimen pretreatment device 2 includes a specimen filter;

the specimen filter includes a body cup 21 and a filter cup 22 nested within the body cup 21;

the side wall of the filter cup 22 and the side wall of the main body cup 21 enclose to form a sampling cavity 25, and the main body cup 21 is provided with a sampling port 24 communicated with the sampling cavity 25;

the bottom of the filter bowl 22 is provided with an opening and is provided with a filter screen 221 in an inclined plane shape, and the inner cavity of the filter bowl 22 is communicated with the sampling cavity 25 through the bottom opening of the filter bowl 22;

the mesh number of the filter screen 221 is 40-120 meshes;

the specimen pretreatment device 2 further includes a first rack 26;

the main body cup 21 is provided with at least one positioning groove 211 formed by inwards sinking;

the first placing frame 26 is provided with a groove 261 for accommodating the specimen filter, and a projection 262 which is arranged in the groove 261 and matched with the positioning groove 211;

the cell transfer device 1 comprises a three-axis mechanical arm 11 and a host 12 connected with the three-axis mechanical arm 11, wherein the host 12 is internally provided with a mounting seat, a rotating shaft 122 and a driving motor 121, the driving motor 121 is in transmission connection with the mounting seat through the rotating shaft 122, and the mounting seat is internally provided with a suction nozzle 123 for connecting the suction head 4;

the positive and negative pressure generating device forms a positive pressure of 5 to 24kPa or a negative pressure of-24 to-20 kPa in the sucker 4;

the cell transfer apparatus 1 further comprises a recovery box 14, a unloading pipette head rack 13 and a wash bowl 15, and a second rack 16 for loading pipette tips 4;

the unloading head frame 13 is provided with a material-removing port 131 which is positioned right above the recovery box 14;

the specimen dyeing device 3 comprises a YZ-axis mechanical arm 31, a dyeing host connected with the YZ-axis mechanical arm 31, a dyeing tank 34 and a third placing rack 33;

the dyeing host comprises a dyeing needle 32;

the dyeing tank 34 is disposed on the third rack 33, and the dyeing tank 34 is located below the dyeing needle 32.

Specifically, the sampling chamber 25 is filled with a preservative solution, and the cleaning tank 15 is filled with purified water. The cell collection brush is now placed in the bowl 22 so that at least the collection head of the brush is immersed in the preservation solution, then the lid 23 is closed and mechanically oscillated during which the diagnostic cells detach from the collection head and pass through the screen 221 into the sampling cavity 25, while most of the interfering components are dissolved or trapped on the screen 221. After the oscillation is completed, the main body cup 21 is placed in the groove 261 of the first placing frame 26 so that the protrusion 262 extends into the positioning groove 211, and at this time, the cup cover 23 is unscrewed to expose the sampling port 24. The triaxial mechanical arm 11 drives the suction head 4 to enable the end face filter membrane 41 to extend into the preservation solution through the sampling port 24, air is blown into the preservation solution through the positive and negative pressure generating device to promote diagnostic cells deposited at the bottom of the main body cup 21 to be resuspended and uniformly dispersed in the preservation solution, then the positive and negative pressure generating device sucks air to form negative pressure, so that part of the preservation solution is sucked into the liquid storage tank through a pipeline, and the diagnostic cells are adsorbed on the filter membrane 41. After the diagnostic cell enrichment is completed, the three-axis mechanical arm 11 draws the suction head 4 out of the sampling port 24 and turns on the driving motor 121 to drive the suction head 4 to swing within a certain angle range through the rotating shaft 122, so that the preservation solution absorbed on the inner wall of the suction head 4 above the filter membrane 41 is cleaned and sucked into the liquid storage tank. Then the filter membrane 41 is extended into the staining bath 34 by the triaxial mechanical arm 11, and the diagnostic cells adsorbed on the filter membrane 41 are released in the staining solution by means of air blowing, and finally the diagnostic cells are drop stained by the specimen staining apparatus 3.

Meanwhile, when the transfer of diagnostic cells is completed, the three-axis robot arm 11 removes the tip 4 through the unloading port 131 provided on the unloading tip holder 13 and drops the tip 4 into the recovery box 14, and then immerses the suction nozzle 123 exposed from the tip 4 in purified water in the cleaning tank 15 to clean the suction nozzle 123 and the internal piping by water suction. Finally the three-axis robot 11 moves the suction nozzle 123 onto the second rack 16 to reload the suction head 4 and await the next process.

Examples of the experiments

The same specimens were separately processed with a conventional TCL slide staining machine (TIB 1800), new berms (TCT,

5000 The staining photographs are shown in fig. 10 to 15, wherein fig. 10 (40 x) and 11 are staining photographs of conventional TLC, fig. 12 (40 x) and 13 are staining photographs of new berms (TCT), and fig. 14 (40 x) and 15 are staining photographs of the liquid-based thin-layer cell slide staining apparatus provided in the present application. As can be seen from the figure, the specimen and the diagnosis cell prepared by the liquid-based thin-layer cell slice staining device provided by the applicationThe structure is complete, and the distribution under the sight glass is even.

The above mentioned is only the embodiment of the present invention, and not the limitation of the patent scope of the present invention, all the equivalent transformations made by the contents of the specification and the drawings, or the direct or indirect application in the related technical field, are included in the patent protection scope of the present invention.

Claims (10)

1. A liquid-based thin-layer cell slice-making and dyeing device is characterized by comprising a specimen pretreatment device, a cell transfer device and a specimen dyeing device which are sequentially arranged along a slice-making and dyeing process; the cell transfer device comprises a suction head with openings at two axial ends and a positive and negative pressure generating device communicated with the suction head through an opening at the upper end of the suction head, and a filter membrane is arranged at an opening at the lower end of the suction head.

2. The apparatus for staining a liquid-based thin-layer cell slide according to claim 1, wherein the specimen pre-processing apparatus includes a specimen filter;

the specimen filter includes a body cup and a filter bowl nested within the body cup;

the side wall of the filter cup and the side wall of the main body cup are enclosed to form a sampling cavity, and a sampling port communicated with the sampling cavity is formed in the main body cup;

the bottom of the filter bowl is provided with an opening and an inclined-plane-shaped filter screen, and the inner cavity of the filter bowl is communicated with the sampling cavity through the bottom opening of the filter bowl.

3. The apparatus for liquid-based thin-layer cell preparation and dyeing according to claim 2, wherein the mesh number of the filter screen is 40-120 meshes.

4. The apparatus for liquid-based thin-layer cell slide-making and staining according to claim 2, wherein the specimen pre-treatment apparatus further comprises a first rack;

the main body cup is provided with at least one positioning groove formed by inwards sinking;

the first placing frame is provided with a groove for accommodating the specimen filter and a protrusion which is arranged in the groove and matched with the positioning groove.

5. The device for liquid-based thin-layer cell slice-making and dyeing according to claim 1, wherein the cell transferring device comprises a three-axis mechanical arm, and a host connected with the three-axis mechanical arm, the host has therein a mounting seat, a rotating shaft and a driving motor, the driving motor is in transmission connection with the mounting seat through the rotating shaft, and the mounting seat has therein a suction nozzle for connecting the suction head.

6. The apparatus for slice-making and staining of liquid-based thin-layer cells as claimed in claim 5, wherein the positive and negative pressure generating means forms a positive pressure of 5 to 24kPa or a negative pressure of-24 to-20 kPa in the suction head.

7. The apparatus of claim 5, further comprising a recycling box, a unloading rack and a wash tank, and a second rack for loading the pipette tips.

8. The apparatus of claim 7, wherein the unloading head frame is provided with a discharging port directly above the recovery box.

9. The apparatus for staining specimen by making liquid-based thin-layer cell slide according to claim 1, wherein the apparatus for staining specimen is LCT staining apparatus.

10. The liquid-based thin-layer cell slice-making and staining device of claim 1, wherein the specimen staining device comprises a YZ-axis mechanical arm, a staining host connected with the YZ-axis mechanical arm, a staining tank, and a third rack;

the dyeing host comprises a dyeing needle;

the dyeing tank is arranged on the third placing frame and is positioned below the dyeing needle.

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