CN218372286U

CN218372286U - Multifunctional periphyton collector

Info

Publication number: CN218372286U
Application number: CN202222767216.3U
Authority: CN
Inventors: 刘炜炜; 谭烨辉; 温少薇; 李开枝
Original assignee: South China Sea Institute of Oceanology of CAS
Current assignee: South China Sea Institute of Oceanology of CAS
Priority date: 2022-10-20
Filing date: 2022-10-20
Publication date: 2023-01-24
Anticipated expiration: 2032-10-20

Abstract

The utility model relates to a multifunctional periphyton collector, wherein a container is a cylinder structure with an upper opening and a lower opening, and the inner cavity wall of the container is provided with a plurality of pairs of slots for fixing glass slides; the top cover is a flat top cover with holes, and the suction filtration adapter is funnel-shaped. The utility model discloses the function is more comprehensive, can stop at the water in a certain time, and the slide glass can obtain multiple peri clump of growth protist kind fully, can survey its variety and community structure developments qualitatively and quantitatively. The collector is exquisite and small, and is convenient to carry. After the original sample is obtained by the collector and brought back to a laboratory, the glass slide can be taken out from the container when the shape is selected for identification, and the shape observation and identification under a microscope are directly carried out on the collected protozoon; when selecting the DNA suction filtration, but adaptation suction filtration device behind the container joint suction filtration adapter, directly carry out the membrane to the sample after the upset from top to bottom and handle and acquire environment DNA, reduce sample processing process's loss, practice thrift a large amount of collection instrument preparation times simultaneously.

Description

Multifunctional periphyton collector

Technical Field

The utility model relates to a multi-functional periclump is born protist collector, but especially direct adaptation suction filtration device acquires environment DNA sample, for aquatic biology taxonomy and the used tool of ecology research field.

Background

The protozoon is an original unicellular organism (the length of the protozoon is about 10 micrometers to 1 millimeter), can be widely distributed in a water body habitat, and has important research value in the aspects of environmental ecology monitoring, breeding disease control and the like; the collection of protists is an important and fundamental link in the research of such organisms. At present, a scouring matrix method is adopted for collecting periphyton in natural water, so that the condition of sample loss exists, and a special environmental DNA in-situ collecting tool is not provided; this makes errors in studies related to investigation of the diversity, community composition, etc. of protists.

SUMMERY OF THE UTILITY MODEL

In order to solve the above problems, the utility model aims to solve the technical problem that the utility model is not enough for the prior art, and provides a multi-functional periphyton collector.

The utility model provides an above-mentioned technical problem's technical scheme as follows:

a multifunctional periphyton collector comprises a container, a top cover, a bottom cover and a suction filtration adapter; the container is vertically arranged and is of a cylinder structure with openings at the upper end and the lower end;

the inner cavity wall of the container is provided with a plurality of slots which are distributed in a central symmetry way, and the slots can be used for inserting the glass slide into the slots to be used as an adhesion matrix of periplex protists.

The bottom cover is matched with the lower end opening of the container and is used during sample collection. The top cover is a flat top cover with a hole, is matched with the opening at the upper end of the container and is used when a sample is collected.

The suction filtration adapter is funnel-shaped, and adaptation suction filtration device uses when carrying out sample DNA filtration, and suction filtration device is preferably suction filtration funnel, suction filtration adapter macrostoma end matches with container upper end opening, and osculum end matches with suction filtration funnel.

On the basis of the technical scheme, the utility model discloses can also do as follows the improvement:

and fixing rings for mounting ropes are arranged on two sides of the container. The bottom cover is provided with a counterweight ring.

The collector is made of PVC.

The beneficial effects of the utility model are that:

the utility model can stay in the water body within a certain time, and can fully obtain the diversity of the protozoon and the dynamic information of community structure; the built-in components can configure the slide to acquire periphyton species. After the sample is collected, the sample is taken back to the laboratory, and the sample can be processed by selecting two modes of morphological identification and DNA suction filtration. When the form is selected for identification, the glass slide can be taken out from the container, and the collected protist is directly subjected to form observation and identification under a microscope. When DNA is selected for suction filtration, the suction filtration converter is used, the container can be adapted to a suction filtration device after being jointed with the suction filtration converter, and the sample is directly subjected to membrane treatment after being turned over up and down to obtain environmental DNA; by combining the form and the molecule, the diversity and community structure dynamics of the biological sample are qualitatively and quantitatively investigated. The collector is exquisite and small, and the cylindrical shell can reduce the acting force of water flow on the collector; conveniently carry, can take the laboratory back to the former state after the collector proposes the surface of water, the sample can directly be preserved in the collector in transit, reduces sample processing's loss, practices thrift a large amount of collection tool preparation times.

Drawings

FIG. 1 is a schematic view of the longitudinal cross-section structure of the utility model in the field sample collection mode;

fig. 2 is a top view of the present invention with the top cover removed.

Fig. 3 is a top view of the top cover of the present invention.

FIG. 4 is the utility model discloses carry out DNA sample suction filtration collection mode in the laboratory longitudinal section structure sketch map down.

FIG. 5 is a perspective view of the present invention in a field sample collection mode;

FIG. 6 is a perspective view of the DNA sample collection system in the laboratory.

In the drawings, the components represented by the respective reference numerals are listed below:

1. the device comprises a container, 2, a slot, 3, a glass slide, 4, a fixing ring, 5, a bottom cover, 6, a top cover, 7, a suction filtration adapter, 8, a suction filtration funnel, 9, a small-mouth end, 10, a large-mouth end, 11, a balance weight ring, 12 and a hole.

Detailed Description

The principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.

As shown in fig. 1 to 6, the utility model relates to a native biological collection ware of multi-functional week clump, the collector includes container 1, bottom 5, top cap 6 and suction filtration adapter 7, the vertical setting of container 1 just is upper and lower end open-ended cylinder structure, and the open end outer wall is equipped with the screw thread, top cap 6, bottom 5 and suction filtration adapter 7 inner wall are equipped with the screw thread.

As shown in fig. 1 and 5, a field sample collection mode is shown. As shown in fig. 3, the surface of the top cover 6 is a net structure with pore size <2 mm to allow the protist to enter the container and prevent the large biological or debris particulate matter from entering the container. The inner wall of the top cover 6 is matched and tightly covered with the upper opening end of the container 1 through threads; the bottom cover 5 is smooth in surface and free of gaps, and the inner wall of the bottom cover is matched and tightly covered with the lower opening end of the container 1 through threads.

The inner cavity wall of the container 1 is provided with slots 2 which are evenly distributed at intervals from top to bottom, before sample collection, a plurality of glass slides 3 are inserted into the slots 2, as shown in figure 2, the plurality of glass slides 3 are arranged in the container 1 in a radial suspension manner in a overlooking angle, and an attachment matrix is formed.

During sampling, the rope is tied on the fixing ring 4, the counterweight is fixed on the counterweight ring 11 of the bottom cover 5, and the container 1 is put into water. The slide 3 can be enriched in the primordial biota of a nutritional and periphyton life (usually capable of forming mini-colony structures) over a period of time.

When a sample is collected in a laboratory, the top cover 6 is taken down, and if morphological observation is carried out, the glass slide 3 can be directly placed in a culture dish for observation and culture of insect bodies after being taken down;

as shown in fig. 4 and fig. 6, if the environmental DNA is obtained, the large-mouth end 10 of the suction filter adapter 7 can be screwed with the upper-mouth end of the container 1 by screw threads, and the small-mouth end 9 of the suction filter adapter 7 is connected with the suction filter funnel 8 by screw threads and put into a filter membrane with a corresponding aperture; turning the container 1 up and down, and taking down the bottom cover 5, so as to carry out membrane filtration on the collected water sample and obtain the environmental DNA.

The utility model discloses can stop in the water in the certain time, dwell time generally is greater than a week, and longer dwell time can obtain primary biodiversity and community structure dynamic information fully.

When the field sample is collected and used, the collector is usually fixed below the water surface of the edge of the ship body at the bank/wharf by a rope for 0.5-1 m, so that the collector can stay below the water surface no matter the tide rises or falls, and the fresh water river or lake habitat is directly fixed at the bank.

The collector is made of PVC; PVC material is not fragile, long service life, easily clean.

In one embodiment of the utility model, the inner diameter of the bottom surface of the container 1 is 7.8cm, and the height is 15cm; the size of the glass slide 3 is 2.5cm multiplied by 7.5cm; the height of the suction filter adapter 7 is 4cm, and the diameter of the small-opening end 9 is 5.5cm.

In the description of the present invention, it should be understood that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.

In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate medium, and may be used for communicating the inside of two elements or for interacting with each other, unless otherwise specifically defined, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to the specific circumstances.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The multifunctional periphyton collector is characterized by comprising a container (1), wherein the container (1) is of a cylindrical structure, the axis line of the container is vertical, and the container is provided with an upper opening and a lower opening; the upper opening is provided with a top cover (6), and the lower opening is provided with a bottom cover (5);

the inner cavity wall of the container (1) is provided with a plurality of pairs of slots (2), two ends of the glass slide (3) are respectively inserted into the slots (2), and the glass slide (3) is used as an adhesion substrate of periphyton;

the top cover (6) is provided with a pore (12) for use in sample collection;

the device also comprises a suction filtration adapter (7) and a suction filtration device, and is used for filtering the DNA of the sample; the suction filtration adapter (7) is funnel-shaped, a large-opening end (10) of the suction filtration adapter (7) is connected with an upper opening of the container (1), and a small-opening end (9) is connected with the suction filtration device.

2. The multifunctional periphyton collector as claimed in claim 1, wherein each pair of slots (2) is symmetrically distributed in the container (1) around the axis of the container (1).

3. The multifunctional periphyton collector according to claim 1, wherein said suction filtration device is a suction filtration funnel (8).

4. The multifunctional periphyton collector according to claim 1, wherein the outer wall of the container (1) is symmetrically provided with a pair of fixing rings (4).

5. The multifunctional periphyton collector as claimed in claim 1, wherein the bottom cover (5) is provided with a weight ring (11).